Membrane separation system for active biological enzyme

By introducing a heating and cooling component into the membrane separator and using a cooling pipe and an electric heater to adjust the temperature, the problem of enzyme inactivation caused by temperature instability in the existing technology is solved, and stable separation of biological enzymes is achieved.

CN223422665UActive Publication Date: 2025-10-10SUZHOU ESTENTE BIOTECHNOLOGY CO LTD

Patent Information

Application Number
CN202422734192.0
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-11-11
Publication Date
2025-10-10
Estimated Expiration
2034-11-11

AI Technical Summary

Technical Problem

Existing membrane separators cannot maintain temperature stability during the separation and purification of biological enzymes, resulting in enzyme inactivation or degradation.

Method used

A system including a membrane separator and a heating and cooling component was designed. The cooling chamber and the heating chamber were formed by the first outer tank and the second outer tank. The temperature was adjusted by a cold injection pipe and an electric heater. The temperature was precisely controlled by combining a temperature sensor and a flow meter.

Benefits of technology

The precise regulation of the membrane separator temperature is achieved, which prevents the biological enzyme from being inactivated or degraded during the separation process and improves the enzyme stability and separation efficiency.

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Abstract

The utility model provides a membrane separation system for an active biological enzyme, and belongs to the technical field of membrane separators. The membrane separation system for the active biological enzyme comprises a membrane separator and a heating and cooling assembly. The heating and cooling assembly comprises a first outer tank, the first outer tank is arranged on the outer side of the membrane separator, the inner wall of the first outer tank and the outer wall of the membrane separator form a cooling cavity, the bottom of the first outer tank is communicated with a cold injection pipe, a second outer tank is arranged outside the first outer tank, and the second outer tank is communicated with the cooling cavity. A heating cavity is formed by the inner wall of the second outer tank and the outer wall of the first outer tank, and an electric heater is installed in the heating cavity. Through the cooling cavity and the heating cavity formed by the first outer tank and the second outer tank, the temperature of the membrane separator can be reduced, so that the temperature can be adjusted according to the temperature requirement during actual biological enzyme membrane separation, and the biological enzyme is prevented from being inactivated or degraded during membrane separation.
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Description

Technical Field

[0001] The present application relates to the field of membrane separators, and in particular to a membrane separation system for active biological enzymes. Background Art

[0002] Enzymes are organic compounds with catalytic properties produced by living cells. Most are proteins, with a small fraction being RNA. The study of their basic properties is called "enzymology." The application of enzymes is called "enzyme engineering." The industrialization of enzymes has led to the development of the enzyme preparation industry, which has permeated various industrial sectors. Enzymes have gained widespread application due to their unique biological functions and high catalytic efficiency. Currently, enzymes are finding increasing application in industries such as medicine, textiles, petroleum, food, and papermaking.

[0003] In the separation and purification of biological enzymes, temperature and pH are two very important factors because they can affect the activity and stability of the enzymes. For example, Chinese application number 202120506704.1 discloses a β-xylosidase separation device that is easy to clean. However, the device cannot heat or cool the biological enzyme, making it impossible to maintain temperature stability during the separation and purification process, thereby causing enzyme inactivation or degradation. Utility Model Content

[0004] In order to make up for the above shortcomings, the present application provides a membrane separation system for active biological enzymes, which aims to improve the problem that general membrane separators cannot heat up or cool down biological enzymes, making it impossible to maintain temperature stability during the separation and purification process, thereby causing enzyme inactivation or degradation.

[0005] An embodiment of the present application provides a membrane separation system for active biological enzymes, including a membrane separator and a heating and cooling component.

[0006] The heating and cooling component includes a first outer tank, which is arranged on the outside of the membrane separator. The inner wall of the first outer tank and the outer wall of the membrane separator form a cooling chamber. The bottom of the first outer tank is connected to a cold injection pipe. A second outer tank is arranged outside the first outer tank. The inner wall of the second outer tank and the outer wall of the first outer tank form a heating chamber. An electric heater is installed inside the heating chamber.

[0007] In a specific embodiment, the outer side wall of the first outer tank is provided with a plurality of grooves at equal intervals, and the wall of each groove does not contact the outer wall of the membrane separator.

[0008] In a specific embodiment, a plurality of heat conducting plates are provided on the side wall of each groove in an annular array, and each of the heat conducting plates is in contact with the outer wall of the membrane separator.

[0009] In a specific embodiment, the electric heater extends into the interior of each of the grooves.

[0010] In a specific embodiment, a temperature sensor is installed on the top of the membrane separator, and a detection end of the temperature sensor is located inside the membrane separator.

[0011] In a specific embodiment, a flow meter is installed on the cold injection pipe.

[0012] In a specific embodiment, the membrane separator is equipped with a membrane separation device.

[0013] In a specific embodiment, a bracket is installed inside the second outer tank, and the electric heater is installed on the bracket and extends into the interior of each of the grooves.

[0014] Beneficial effect: The cooling chamber and the heating chamber formed by the first outer tank and the second outer tank can not only cool down the membrane separator but also heat it up, so that it can be adjusted according to the actual temperature requirements during biological enzyme membrane separation, preventing the biological enzyme from being inactivated or degraded during membrane separation. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the technical solutions of the implementation methods of the present application, the following is a brief introduction to the drawings required for use in the implementation methods. It should be understood that the following drawings only show certain embodiments of the present application and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying any creative work.

[0016] Figure 1 This is a schematic diagram of the structure of a membrane separation system for active biological enzymes provided in an embodiment of the present application;

[0017] Figure 2 A schematic diagram of the heating and cooling assembly structure provided in an embodiment of the present application;

[0018] Figure 3 Provided for the implementation of this application Figure 2 A in the middle is an enlarged structural diagram;

[0019] Figure 4 A schematic diagram of the structure of the second outer tank provided in an embodiment of the present application;

[0020] Figure 5 This is a schematic diagram of the first outer tank structure provided in an embodiment of the present application.

[0021] In the figure: 10 - membrane separator; 20 - heating and cooling assembly; 210 - first outer tank; 220 - groove; 230 - heat conduction plate; 240 - second outer tank; 250 - electric heater; 260 - cooling pipe; 270 - temperature sensor. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the present application will be described below in conjunction with the drawings in the embodiments of the present application.

[0023] See also Figure 1-Figure 5 The present application provides a membrane separation system for active biological enzymes, including a membrane separator 10 and a heating and cooling component 20.

[0024] See also Figure 1 、 Figure 2 and Figure 5 The membrane separator 10 utilizes natural or artificially synthesized polymer membranes to separate, grade, purify and enrich two-component or multi-component solutes and solvents with the driving force of external energy or chemical potential difference, thereby achieving membrane separation of active biological enzymes.

[0025] See also Figure 1 、 Figure 2 、 Figure 3 、 Figure 4 and Figure 5 The heating and cooling assembly 20 includes a first outer tank 210, which is disposed outside the membrane separator 10. The inner wall of the first outer tank 210 and the outer wall of the membrane separator 10 form a cooling chamber. The bottom of the first outer tank 210 is connected to a cold injection pipe 260. Specifically, liquid nitrogen or other cold substances that can reduce the temperature can be injected through the cold injection pipe 260. A second outer tank 240 is disposed outside the first outer tank 210. The inner wall of the second outer tank 240 and the outer wall of the first outer tank 210 form a heating chamber. An electric heater 250 is installed inside the heating chamber. Specifically, the electric heater 250 can be an electric heating wire or an electric heating tube. When the active biological enzyme needs to be cooled during membrane separation in the membrane separator 10, a cold substance is injected through the cold injection pipe 260 to reduce the temperature inside the cooling chamber. When the temperature inside the cooling chamber decreases, the temperature inside the membrane separator 10 also decreases. When the temperature needs to be increased, the injection of the cold substance is stopped, and then the electric heater 250 is started to increase the temperature inside the heating chamber, thereby increasing the temperature inside the membrane separator 10. The cooling chamber and the heating chamber formed by the first outer tank 210 and the second outer tank 240 can not only cool the membrane separator 10 but also increase the temperature, so that the temperature can be adjusted according to the actual temperature requirements during the biological enzyme membrane separation, preventing the biological enzyme from being inactivated or degraded during the membrane separation.

[0026] In this embodiment, the outer wall of the first outer tank 210 is provided with a plurality of grooves 220 at equal intervals, and the walls of each groove 220 do not contact the outer wall of the membrane separator 10. The provision of each groove 220 brings the heating chamber closer to the membrane separator 10, thereby improving the heating effect. Furthermore, the absence of contact between the walls of each groove 220 and the outer wall of the membrane separator 10 ensures flow in the cooling chamber.

[0027] In this embodiment, a plurality of heat conducting plates 230 are provided in a circular array on the sidewall of each groove 220, and each heat conducting plate 230 is in contact with the outer wall of the membrane separator 10. The heat conducting plates 230 can quickly transfer heat from the heating chamber to the membrane separator 10, further improving the heating effect.

[0028] In a specific configuration, the electric heater 250 extends to the inside of each groove 220 . A bracket is installed inside the second outer tank 240 , and the electric heater 250 is installed on the bracket and extends to the inside of each groove 220 .

[0029] A temperature sensor 270 is installed on the top of the membrane separator 10, and the detection end of the temperature sensor 270 is located inside the membrane separator 10. The temperature sensor 270 can monitor the temperature inside the membrane separator 10 in real time, so that the required temperature can be known at any time.

[0030] A flow meter is installed on the cold injection pipe 260. Among them, the injected cold material can be calculated.

[0031] The membrane separator 10 is equipped with a membrane separation device.

[0032] The working principle of the membrane separation system for active biological enzymes:

[0033] When the active biological enzyme needs to be cooled during membrane separation in the membrane separator 10, a cold substance is injected through the cold injection pipe 260 to reduce the temperature inside the cooling chamber. When the temperature inside the cooling chamber decreases, the temperature inside the membrane separator 10 also decreases. When the temperature needs to be increased, the injection of the cold substance is stopped, and then the electric heater 250 is started to increase the temperature inside the heating chamber, thereby increasing the temperature inside the membrane separator 10. The cooling chamber and the heating chamber formed by the first outer tank 210 and the second outer tank 240 can not only cool the membrane separator 10 but also increase the temperature, so that the temperature can be adjusted according to the actual temperature requirements during the biological enzyme membrane separation, preventing the biological enzyme from being inactivated or degraded during the membrane separation.

[0034] It should be noted that the specific models and specifications of the electric heater 250, temperature sensor 270 and flow meter need to be selected and determined based on the actual specifications of the device. The specific selection calculation method adopts the existing technology in this field, so it will not be described in detail.

[0035] The power supply and principles of the electric heater 250 , the temperature sensor 270 and the flow meter are clear to those skilled in the art and will not be described in detail here.

[0036] The foregoing is merely an embodiment of the present application and is not intended to limit the scope of protection of the present application. Various modifications and variations are possible for those skilled in the art. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present application shall be included within the scope of protection of the present application. It should be noted that similar reference numerals and letters represent similar items in the following figures. Therefore, once an item is defined in one figure, it does not need to be further defined or explained in subsequent figures.

Claims

1. A membrane separation system for active biological enzymes, characterized in that: include Membrane separator (10), A heating and cooling component (20) comprises a first outer tank (210), wherein the first outer tank (210) is arranged outside the membrane separator (10), the inner wall of the first outer tank (210) and the outer wall of the membrane separator (10) form a cooling chamber, the bottom of the first outer tank (210) is connected to a cold injection pipe (260), a second outer tank (240) is arranged outside the first outer tank (210), the inner wall of the second outer tank (240) and the outer wall of the first outer tank (210) form a heating chamber, and an electric heater (250) is installed inside the heating chamber.

2. A membrane separation system for active biological enzymes according to claim 1, characterized in that: The outer side wall of the first outer tank (210) is provided with a plurality of grooves (220) at equal intervals, and the wall of each groove (220) does not contact the outer wall of the membrane separator (10).

3. A membrane separation system for active biological enzymes according to claim 2, characterized in that: A plurality of heat conducting plates (230) are provided on the side wall of each groove (220) in an annular array, and each heat conducting plate (230) is in contact with the outer wall of the membrane separator (10).

4. A membrane separation system for active biological enzymes according to claim 2, characterized in that: The electric heater (250) extends into the interior of each of the grooves (220).

5. A membrane separation system for active biological enzymes according to claim 1, characterized in that: A temperature sensor (270) is installed on the top of the membrane separator (10), and a detection end of the temperature sensor (270) is located inside the membrane separator (10).

6. A membrane separation system for active biological enzymes according to claim 1, characterized in that: A flow meter is installed on the cold injection pipe (260).

7. A membrane separation system for active biological enzymes according to claim 1, characterized in that: The membrane separator (10) is equipped with a membrane separation device.

8. A membrane separation system for active biological enzymes according to claim 2, characterized in that: A bracket is installed inside the second outer tank (240), and the electric heater (250) is installed on the bracket and extends into the interior of each groove (220).

Citation Information

Patent Citations

  • Beta-xylosidase separation device convenient to clean

    CN214865892U

Cited By

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