Simple cell plunger pump

Abstract

The utility model relates to the field of cell culture, in particular to a simple cell plunger pump which comprises a barrel, a filter element and a push plug. The cylinder is in a hollow column shape with two open ends and is provided with a first end and a second end which are opposite. The filter element comprises a reinforcing part, a connecting part and a filter screen. A plurality of through holes are formed in the reinforcing part, the connecting part is connected to the edge of the reinforcing part to form a box-shaped structure, and the connecting part is matched with the cylinder body and is detachably connected to the first end of the cylinder body in a sealing manner. The filter screen is attached and connected to the side surface, close to the cylinder, of the reinforcing part, covers all the through holes of the reinforcing part, intercepts cells and permeates a solution. The push plug comprises a plug body and a push rod which are mutually connected. And the plug body is accommodated in the barrel body and is matched with the inner walls of the barrel body and the filter element. The push rod is connected to the end, away from the filter screen, of the plug and used for driving the plug to move in the direction parallel to the barrel. Separation of cells and a solution is achieved through the filter screen, the filter element is detached to collect the cells, and cell loss can be reduced.

Description

Technical Field

[0001] The utility model relates to the field of cell culture, in particular to a simple cell plunger pump. Background Art

[0002] In biological research, cell culture and separation are often required. After cells are added to a culture medium and fully incubated, a culture medium containing a large number of cells is obtained. To separate the culture medium from the cells, centrifugation is usually performed to allow the cells to settle at the bottom of the centrifuge tube. The supernatant is then discarded, completing the separation of the culture medium from the cells.

[0003] However, after centrifugation, the supernatant still contains a large number of cells, and discarding the supernatant will result in cell waste. When cell samples are precious, cell yield will directly affect the subsequent experimental process. Utility Model Content

[0004] The purpose of the utility model is to provide a cell collection device with less cell loss.

[0005] In order to achieve the above-mentioned purpose, the present invention provides the following technical solutions:

[0006] A simple cell plunger pump, comprising:

[0007] The cylinder is constructed in the shape of a hollow column with two ends open, and has a first end and a second end opposite to the first end, wherein the first end of the cylinder is formed with;

[0008] A filter element comprising a reinforcement portion, a connecting portion, and a filter screen. The reinforcement portion is formed with a plurality of through holes. The connecting portion is fitted with the barrel and connected to the edge of the reinforcement portion to form a box shape. An end of the connecting portion away from the reinforcement portion is detachably connected to the first end of the barrel, and the connecting portion and the barrel are sealed. The filter screen is attached to the side of the reinforcement portion close to the barrel and covers all the through holes of the reinforcement portion, and is used to intercept cells and allow the permeation of solution.

[0009] The push plug includes a plug body and a push rod connected to each other. The plug body is accommodated in the cylinder, and the outer wall of the plug body is tightly fitted with the inner wall of the cylinder and / or the inner wall of the connecting part of the filter element. The structure of the end of the plug body close to the filter element is matched with the reinforcement part of the filter element. The push rod is connected to the end of the plug body away from the filter screen, and is used to drive the plug body to move in a direction parallel to the cylinder.

[0010] Optionally, an inner wall of the second end of the cylinder is formed with a limiting portion protruding inward, and a liquid inlet is arranged on the side wall of the cylinder to communicate the inside and outside of the cylinder, and the sampling position is the position where the plug body abuts against the limiting portion of the cylinder, and the liquid inlet is arranged between the filter element and the sampling position.

[0011] Optionally, the cylinder is further provided with an air outlet and a rubber plug matched with the air outlet, and the cylinder is marked with a water filling line for indicating the maximum water filling amount, the liquid inlet is arranged between the water filling line and the sampling position, the air outlet is arranged between the liquid inlet and the water filling line, and the rubber plug is used to seal the air outlet.

[0012] Optionally, the simple cell plunger pump further comprises an auxiliary mechanism, the auxiliary mechanism comprises a driving motor, a connecting assembly and a support, the support is used to support the cylinder and the filter element connected with each other, the connecting assembly is used to connect the driving motor and the support, the connecting assembly is parallel to the cylinder, and the driving motor is used to drive the push rod to move in the direction parallel to the cylinder.

[0013] Optionally, an end of the push rod away from the plug body is connected with a push-pull portion, the push-pull portion is plate-shaped and perpendicular to the cylinder, and a middle portion of a side bottom surface of the push-pull portion is connected to the push rod, and an abutting portion extending outward is connected to an outer wall of the filter element.

[0014] Optionally, the driving motor has a free end controllably displaced, the free end of the driving motor is connected with a constraint member, the constraint member comprises push plates and pull plates parallel to the push-pull portion, the push plates and the pull plates are oppositely arranged and connected with each other, two sets of the push plates and the pull plates arranged in alignment are separated from each other and arranged on two sides of the push rod respectively, and two ends of the push-pull portion are respectively inserted between the two sets of the push plates and the pull plates and displaced with the movement of the constraint member.

[0015] Optionally, the support comprises support plates and blocking plates, the support plates and the blocking plates are connected with each other and arranged on upper and lower sides of the abutting portion of the filter element respectively, and used to constrain the position of the filter element, and two sets of the support plates and the blocking plates arranged in alignment are separated from each other and arranged on two sides of the filter element respectively.

[0016] Optionally, the auxiliary mechanism further comprises a vertical rod and a support clamp, the vertical rod is vertically arranged, the support clamp comprises a rod clamp and a cylinder clamp, the rod clamp is fixedly connected to the vertical rod, and the cylinder clamp is connected to the cylinder clamp and used to clamp the cylinder.

[0017] Optionally, the connecting assembly is controllably telescopic.

[0018] Optionally, the connecting assembly includes a mother rod and a sub-rod slidably connected to the mother rod, the mother rod is formed with a plurality of first connecting holes arranged in a direction parallel to the mother rod, the sub-rod is formed with second connecting holes that match the first connecting holes, and the second connecting holes are detachably connected to any of the first connecting holes by bolts.

[0019] The beneficial effects of the present invention are that the filter screen intercepts cells, helping to reduce cell loss and improve cell yield. Because the barrel, filter element, and push plug all have relatively simple structures, they can be constructed as disposable consumables, helping to reduce the risk of biological contamination. The detachable connection of the filter element to the barrel facilitates the selection of filter elements with different pore sizes based on cell type and facilitates the collection of filtered cells, thus offering high practicality.

[0020] The above description is only an overview of the technical solution of the present invention. In order to more clearly understand the technical means of the present invention and to implement it according to the contents of the specification, the following is a detailed description of the preferred embodiments of the present invention in conjunction with the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a schematic structural diagram of the simple cell plunger pump shown in Example 1 of the present utility model;

[0022] Figure 2 for Figure 1 A magnified schematic diagram of point A in the middle;

[0023] Figure 3 for Figure 1 Enlarged schematic diagram of point B in the middle.

[0024] Legend: 1-cylinder, 11-first end, 12-second end, 13-rubber ring, 14-limiting part, 15-water injection line, 16-liquid inlet, 17-air outlet, 18-rubber plug, 2-filter element, 21-reinforcement part, 211-through hole, 22-connecting part, 23-filter screen, 24-contact part, 3-push plug, 31-plug body, 32-push rod, 33-push-pull part, 41-drive motor, 4 11-free end, 412-constraint, 413-push plate, 414-vertical plate, 415-pull plate, 416-blocking part, 417-fixed end, 42-connecting assembly, 421-mother rod, 422-sliding track, 423-first connecting hole, 424-sub-rod, 425-bolt, 43-support member, 431-structural plate, 432-support plate, 433-blocking plate, 441-support clamp. DETAILED DESCRIPTION

[0025] The following is a clear and complete description of the technical solution of the present invention in conjunction with the accompanying drawings. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0026] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating positions or relationships, are based on the positions or relationships shown in the accompanying drawings and are intended solely to facilitate the description of this utility model and simplify the description. They do not indicate or imply that the devices or components referred to must have a specific orientation, be constructed, or operate in a specific orientation. Therefore, they should not be construed as limitations on this utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0027] In the description of this utility model, it should be noted that, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" should be understood in a broad sense. For example, they can refer to fixed connections, detachable connections, or integral connections; mechanical connections, electrical connections; direct connections, indirect connections through an intermediate medium, and internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on the specific circumstances.

[0028] In addition, the technical features involved in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0029] See Figure 1The simple cell plunger pump shown in the preferred embodiment of the present application comprises a barrel 1, a filter core 2 and a push plug 3. The barrel 1 is configured as a hollow cylindrical shape with open ends, having a first end 11 and a second end 12 opposite to the first end 11. The filter core 2 comprises a reinforcing part 21, a connecting part 22 and a filter screen 23. The reinforcing part 21 is formed with a plurality of through holes 211, the connecting part 22 is connected to the edge of the reinforcing part 21 to form a box-shaped structure, and the connecting part 22 is fitted into the barrel 1 and detachably connected to the first end 11 of the barrel 1 in a sealed manner. The filter screen 23 is attached to the side of the reinforcing part 21 close to the barrel 1, covering all the through holes 211 of the reinforcing part 21, intercepting cells and allowing the solution to pass through. The push plug 3 comprises a plug body 31 and a push rod 32 connected to each other. The plug body 31 is accommodated in the barrel 1, and the outer wall thereof is tightly attached to the inner wall of the barrel 1 and / or the connecting part 22. The end of the plug body 31 close to the filter core 2 is configured to fit into the reinforcing part 21 of the filter core 2. The push rod 32 is connected to the end of the plug body 31 away from the filter screen 23, for driving the plug body 31 to move in a direction parallel to the barrel 1.

[0030] The cells are intercepted by the filter screen 23, which helps to reduce the loss of cells and improve the yield of cells. Since the structures of the barrel 1, the filter core 2 and the push plug 3 are relatively simple, they can be configured as disposable consumables, which helps to reduce the risk of biological contamination. The detachable connection of the filter core 2 to the barrel 1 facilitates the selection of filter cores 2 with different pore sizes according to the types of cells, and facilitates the collection of the filtered cells, which has high practicability.

[0031] The cells in the utility model comprise bacterial bodies, and specific reference can be made to the following examples.

[0032] Example 1

[0033] Please refer to Figure 1 , Figure 2 and Figure 3 , the simple cell plunger pump shown in the preferred embodiment of the present application comprises a barrel 1, a filter core 2 and a push plug 3.

[0034] The barrel 1 is configured as a hollow cylindrical shape with open ends, having a first end 11 and a second end 12 opposite to the first end 11. The outer wall of the first end 11 of the barrel 1 is raised in a stepped shape along the direction parallel to the barrel 1, and the inner wall of the raised part is formed with threads. The first end 11 of the barrel 1 is fixedly connected with a rubber ring 13, and the rubber ring 13 is sleeved on the inner side of the raised part. The inner wall of the second end 12 of the barrel 1 is raised inward to form a circular annular limiting part 14. The middle part of the barrel 1 is marked with a water injection line 15 for indicating the maximum liquid injection amount. The side wall of the barrel 1 is provided with a liquid inlet 16 and a gas outlet 17 communicating between the inside and outside of the barrel 1. The liquid inlet 16 is arranged between the water injection line 15 and the limiting part 14, the gas outlet 17 is arranged between the liquid inlet 16 and the water injection line 15, and is arranged on the side opposite to the liquid inlet 16. The gas outlet 17 is controllably sealed by a rubber plug 18 fitted into the gas outlet 17.

[0035] The filter element 2 includes a reinforcement portion 21, a connecting portion 22 and a filter screen 23. The connecting portion 22 is constructed to form a cylindrical shape with the inner and outer walls having the same diameter as the cylinder 1. The inner wall of one end thereof is raised in a direction parallel to the connecting portion 22 and forms a thread, so that the structure of the connecting portion 22 is matched with the first end 11 of the cylinder 1. The connecting portion 22 is detachably connected to the first end 11 of the cylinder 1 by screwing, and a sealed connection between the filter element 2 and the cylinder 1 is achieved by a rubber ring 13. The end of the connecting portion 22 away from the cylinder 1 is closed by a circular plate-shaped reinforcement portion 21, and a plurality of evenly distributed through holes 211 are formed on the reinforcement portion 21. The filter screen 23 is fitted and connected to the side of the reinforcement portion 21 close to the cylinder 1, and maintains its structure under the support of the reinforcement portion 21 to prevent damage. The filter screen 23 covers all the through holes 211 of the reinforcement portion 21, intercepts cells and permeates the solution, and ensures that all liquid passing through the filter element 2 passes through the filter screen 23. The side wall of the filter element 2 protrudes outward and extends to form a resistance portion 24 . The resistance portion 24 in this embodiment is configured as a long strip structure perpendicular to the cylinder 1 , and two resistance portions 24 are formed on both sides of the filter element 2 .

[0036] The push plug 3 includes a plug body 31, a push rod 32 and a push-pull portion 33. The cylindrical plug body 31 is accommodated in the cylinder 1, and the structure of the end of the plug body 31 close to the filter element 2 is matched with the reinforcement portion 21 of the filter element 2, and the diameter is matched with the inner wall of the cylinder 1. The thickness of the plug body 31 along the direction parallel to the cylinder 1 is less than the distance between the liquid inlet 16 and the limit portion 14, and the spaces on both sides of the plug body 31 in the cylinder 1 are not connected to each other. The cylindrical push rod 32 is connected to the end of the plug body 31 away from the filter screen 23, and is used to drive the plug body 31 to move in a direction parallel to the cylinder 1. The push rod 32 is connected to the end away from the plug body 31 with a push-pull portion 33, which is constructed as a circular plate-like structure, coaxial with the plug body 31 and the push rod 32, and the diameter of the push-pull portion 33 is larger than the diameter of the push rod 32.

[0037] When the culture fluid is separated manually, a filter element 2 with a pore size of the filter screen 23 that matches the cell diameter is selected and connected to the cylinder 1. The position where the plug 31 contacts the limit portion 14 of the cylinder 1 is the injection position. The plug 31 is pulled by the push rod 32 or the push-pull portion 33 so that the plug 31 is in the injection position. The rubber plug 18 is removed to open the air outlet 17, and the culture fluid is added to the cylinder 1 from the liquid inlet 16 so that the liquid level of the culture fluid is below the water injection line 15. Push the push rod 32 or the push-pull portion 33 to bring the plug 31 close to the filter screen 23. After the plug 31 passes the liquid inlet 16, the rubber plug 18 is tightened to close the air outlet 17, and the plug 31 is continued to be pushed. When the plug 31 contacts the filter screen 23, the filtration is completed. When it is necessary to add buffer to wash the cells, repeat the above steps to place the plug 31 in the injection position and add buffer to filter again. When it is difficult to continue pushing the plug 31 during the filtering process, the cylinder 1 is inverted so that the filter element 2 is located above the cylinder 1 so that there is no more liquid in the filter element 2. The filter element 2 is removed and stored, and then a new filter element 2 is installed.

[0038] After the cells are enriched in the filter element 2, the solid matter in the filter element 2 is sucked out, and the end of the plunger close to the filter element 2 is flushed with buffer to collect the washing liquid. Buffer is added to the filter element 2 and blown to collect until the filter element 2 can no longer retain the buffer. The filter element 2 is then floated on the liquid surface of the buffer. Buffer is added to the filter element 2 and stirred to collect several times. The obtained solid matter is resuspended in a combined solution of multiple washing liquids to obtain a cell resuspension.

[0039] The simple cell plunger pump in this embodiment further includes an auxiliary mechanism, which includes an iron frame, a drive motor 41 , a connecting assembly 42 and a support member 43 .

[0040] The iron stand comprises a vertical pole and a support clamp 441. The vertical pole is vertically arranged. The support clamp 441 comprises a rod clamp and a collet clamp. The rod clamp is fastened to the vertical pole and the collet clamp is connected to the collet clamp for clamping the cylinder 1 and keeping the cylinder 1 vertical. Since the structure of the iron stand is prior art, it will not be described here in detail.

[0041] The drive motor 41 is fixedly connected to the vertical rod, and its free end 411 is controllably movable in the vertical direction. The free end 411 of the drive motor 41 is connected to a constraint 412, which includes a horizontally arranged push plate 413 and a pull plate 415, as well as a vertically arranged vertical plate 414. The edges of the push plate 413 and the vertical plate 414 are connected to each other to form a right angle, and the vertical plate 414 is located below the push plate 413. The bottom edges of the vertical plate 414 extend horizontally to form a pull plate 415 opposite to the push plate 413. The distance between the two pull plates 415 is greater than the diameter of the push rod 32 and less than the diameter of the push-pull portion 33. The end of the pull plate 415 away from the vertical plate 414 is vertically bent upward to form a blocking portion 416, and a vertically downward blocking portion 416 is formed at a position on the push plate 413 opposite to the pull plate 415. The height of the gap between the push plate 413 and the blocking portion 416 of the pull plate 415 is greater than the thickness of the push-pull portion 33. The push-pull portion 33 is inserted through the gap to reach above the pull plate 415 and supported by the pull plate 415. When the free end 411 of the drive motor 41 rises, the pull plate 415 drives the push plug 3 to rise. When the free end 411 of the drive motor 41 descends, the push plate 413 drives the push plug 3 to descend.

[0042] The connecting assembly 42 includes a mother rod 421 and a sub-rod 424. The two long mother rods 421 are arranged vertically, connected to the fixed end 417 of the drive motor 41 and are respectively located on both sides of the constraint 412. The edges on both sides of the mother rod 421 are bent inward to form a sliding track 422 parallel to the mother rod 421. A plurality of first connecting holes 423 arranged in the vertical direction and connected to each other are formed in the middle of the mother rod 421. The sub-rod 424 is slidably connected to the mother rod 421, and a second connecting hole that matches the first connecting hole 423 is formed on the sub-rod 424. The bolt 425 is detachably connected to the second connecting hole and any one of the first connecting holes 423, so that the connecting assembly 42 can be extended and retracted in a controllable manner.

[0043] The support member 43 is connected to the end of the sub-rod 424 away from the drive motor 41, and includes a structural plate 431, a support plate 432 and a baffle 433. The structural plate 431 is vertically arranged and has a rectangular structure, and is connected to the two sub-rods 424 at the same time. The two support plates 432 are horizontally connected to the bottom of the structural plate 431, and are bent vertically upward at one end away from the structural plate 431. The two support plates 432 are respectively arranged at both ends of the structural plate 431 in the horizontal direction, and the distance between the two support plates 432 is greater than the diameter of the connecting portion 22 of the filter element 2, and is less than the distance between the two interference portions 24 at one end away from the connecting portion 22. The two baffles 433 are respectively arranged directly above the two support plates 432, and are bent vertically downward at one end away from the structural plate 431. The interference portion 24 of the filter element 2 is inserted between the support plate 432 and the baffle 433, thereby limiting the filter element 2.

[0044] When an auxiliary mechanism is used to assist in cell separation, the barrel 1, filter element 2, and push plug 3 are assembled and then installed on the auxiliary mechanism. After the plug 31 is positioned at the injection position, the liquid inlet 16 is connected to the liquid tank via a hose. The culture medium in the liquid tank is injected into the barrel 1 under the action of positive pressure. When the liquid level in the barrel 1 approaches the water injection line 15, the air outlet 17 is closed by the rubber plug 18, and the liquid injection is continued until the culture medium is completely injected. At this point, the culture medium is concentrated, and the push plug 3 is pushed by the drive motor 41 to complete the separation of the cells and the culture medium.

[0045] In this embodiment, the barrel 1, the filter element 2 and the push plug 3 are all disposable consumables and are discarded after use to prevent biological contamination.

[0046] Example 2:

[0047] The only difference between this embodiment and the first embodiment is that the interference portion 24 and the support member 43 are not provided in this embodiment, and the cylinder 1 is fixed by the support clip 441 .

[0048] Example 3:

[0049] The only difference between this embodiment and the second embodiment is that the restraining member 412 in this embodiment is a horizontally arranged spring clip.

[0050] The technical features of the above-mentioned embodiments can be combined arbitrarily. In order to make the description concise, not all possible combinations of the technical features in the above-mentioned embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0051] The above-described embodiments merely represent several implementations of the present invention. While the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the utility model patent. It should be noted that a person skilled in the art would be able to make various modifications and improvements without departing from the concept of the present invention, and these modifications and improvements fall within the scope of protection of the present invention. Therefore, the scope of protection of the present utility model patent shall be determined by the appended claims.

Claims

1. A simple cell plunger pump, characterized in that: include: The cylinder (1) is constructed in the shape of a hollow column with both ends open, and has a first end (11) and a second end (12) opposite to the first end (11); A filter element (2) comprises a reinforcement portion (21), a connection portion (22) and a filter screen (23), wherein the reinforcement portion (21) is formed with a plurality of through holes (211), the connection portion (22) is fitted with the barrel (1) and connected to the edge of the reinforcement portion (21) to form a box shape, an end of the connection portion (22) away from the reinforcement portion (21) is detachably connected to the first end (11) of the barrel (1), and the connection portion (22) and the barrel (1) are sealed, the filter screen (23) is fitted and connected to the side of the reinforcement portion (21) close to the barrel (1), and covers all the through holes (211) of the reinforcement portion (21), and is used to intercept cells and pass through a solution; The push plug (3) comprises a plug body (31) and a push rod (32) connected to each other, wherein the plug body (31) is accommodated in the cylinder (1), and the outer wall of the plug body (31) is tightly fitted with the inner wall of the cylinder (1) and / or the inner wall of the connecting portion (22) of the filter element (2), the structure of one end of the plug body (31) close to the filter element (2) is matched with the reinforcing portion (21) of the filter element (2), and the push rod (32) is connected to the end of the plug body (31) away from the filter screen (23) and is used to drive the plug body (31) to move in a direction parallel to the cylinder (1).

2. The simple cell plunger pump according to claim 1, wherein A limiting portion (14) protruding inward is formed on the inner wall of the second end (12) of the cylinder (1), and a liquid inlet (16) communicating with the inside and outside of the cylinder (1) is provided on the side wall of the cylinder (1). The position when the plug (31) contacts the limiting portion (14) of the cylinder (1) is the injection position, and the liquid inlet (16) is provided between the filter element (2) and the injection position.

3. The simple cell plunger pump according to claim 2, characterized in that The cylinder (1) is also provided with an air outlet (17) and a rubber plug (18) matched with the air outlet (17); the cylinder (1) is marked with a water injection line (15) for indicating the maximum injection amount; the liquid inlet (16) is provided between the water injection line (15) and the injection position; the air outlet (17) is provided between the liquid inlet (16) and the water injection line (15); and the rubber plug (18) is used to seal the air outlet (17).

4. The simple cell plunger pump according to claim 1, wherein The invention also includes an auxiliary mechanism, which includes a driving motor (41), a connecting assembly (42) and a supporting member (43), wherein the supporting member (43) is used to support the cylinder (1) and the filter element (2) connected to each other, the connecting assembly (42) is used to connect the driving motor (41) and the supporting member (43), and the connecting assembly (42) is parallel to the cylinder (1), and the driving motor (41) is used to push the push rod (32) to move in a direction parallel to the cylinder (1).

5. The simple cell plunger pump according to claim 4, characterized in that The push rod (32) is connected to a push-pull portion (33) at one end away from the plug body (31). The push-pull portion (33) is constructed in a plate shape perpendicular to the cylinder body (1), and the middle part of the bottom surface on one side is connected to the push rod (32). The outer wall of the filter element (2) is connected to a contact portion (24) extending toward the outside.

6. The simple cell plunger pump according to claim 5, characterized in that The driving motor (41) has a free end (411) that can be displaced in a controllable manner. The free end (411) of the driving motor (41) is connected to a restraining member (412). The restraining member (412) includes a push plate (413) and a pull plate (415) that are parallel to the push-pull portion (33). The push plate (413) and the pull plate (415) are arranged relative to each other and connected to each other. Two groups of flush-arranged push plates (413) and pull plates (415) are separated from each other and are respectively arranged on both sides of the push rod (32). The two ends of the push-pull portion (33) are respectively inserted between the two groups of push plates (413) and the pull plates (415) and are displaced as the restraining member (412) moves.

7. The simple cell plunger pump according to claim 5, characterized in that The support member (43) comprises a support plate (432) and a baffle plate (433), the support plate (432) and the baffle plate (433) being connected to each other and respectively arranged on the upper and lower sides of the abutting portion (24) of the filter element (2) for constraining the position of the filter element (2), and two groups of the support plates (432) and the baffle plates (433) being arranged flush with each other are separated from each other and respectively arranged on both sides of the filter element (2).

8. The simple cell plunger pump according to claim 4, characterized in that The auxiliary mechanism also includes a vertical rod and a support clamp (441), wherein the vertical rod is vertically arranged, and the support clamp (441) includes a rod clamp and a tube clamp, wherein the rod clamp is firmly connected to the vertical rod, and the tube clamp is connected to the tube clamp for clamping the cylinder (1).

9. The simple cell plunger pump according to claim 4, characterized in that The connecting assembly (42) is controllably extendable and retractable.

10. The simple cell plunger pump according to claim 9, characterized in that The connecting assembly (42) includes a mother rod (421) and a sub-rod (424) slidably connected to the mother rod (421), wherein the mother rod (421) is formed with a plurality of first connecting holes (423) arranged in a direction parallel to the mother rod (421), and the sub-rod (424) is formed with a second connecting hole matched with the first connecting hole (423), and the second connecting hole is detachably connected to any of the first connecting holes (423) by a bolt (425).

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