Full-automatic multifunctional pilot platform system and chromatographic system thereof

Abstract

The utility model provides a full-automatic multifunctional pilot platform system and a chromatographic system thereof. The platform system comprises a first input main pipeline with a first input pump and a second input main pipeline with a second input pump, the first input main pipeline is connected with a plurality of input pipelines, and on-off of the first input main pipeline is controlled through corresponding valves; the second input main pipeline is also respectively connected with the plurality of input pipelines, and the on-off of the second input main pipeline is controlled through corresponding valves; the outlet ends of the input pipelines are connected with liquid inlet ports of the corresponding movable installation modules respectively, and the movable installation modules are further provided with a plurality of backflow ports and a plurality of liquid outlet ports. The liquid outlet port is respectively connected with a plurality of collecting pipelines through output pipelines; according to the chromatographic system, an RTF column is mounted in a movable mounting module; during use, two tangential flow chromatographic columns are adopted and operate alternately: one is in a loading mode, and the other is in an elution mode; the multi-functional biological treatment device has the advantages that modularization is achieved, multiple functions are achieved according to different process requirements, and the downstream production process of biological medicine is met.

Description

Technical Field

[0001] The utility model relates to a biomedicine downstream platform, in particular to a full-automatic multifunctional pilot platform system and a chromatography system thereof. Background Art

[0002] Downstream biopharmaceutical processes include filtration, which is primarily used to remove impurities such as cell debris, bacteria, and viruses. Filtration includes both NFF and TFF; and purification, which is primarily used to separate, purify, and refine the target product, involving the use of chromatography columns.

[0003] In the prior art, for example, Patent No. 202120067489.X, the utility model name is a hydraulically driven dynamic chromatography device. When the separation liquid needs to be added, the liquid is injected from the liquid inlet and flows out from the liquid outlet for collection; or, for example, Patent No. 201820028656.8, the utility model name is a moving beam type fully automatic chromatography column. The structure adopted at the bottom is a sieve plate, a diverter plate and a base. A lower nozzle is provided at the center of the sieve plate. When the separation liquid needs to be added, the liquid enters from the chromatography port of the upper nozzle and flows out from the chromatography port of the lower nozzle for collection, or enters from the chromatography port of the lower nozzle and flows out from the chromatography port of the upper nozzle for collection. Its disadvantages are: 1. It cannot achieve flushing or elution of the upper part of the column head; 2. It does not support backwashing of the filter plate (sieve plate), which can easily lead to blockage; 3. It cannot achieve high linear flow rate and overload loading at the same time, and the dynamic loading capacity is low. The applicant has proposed a tangential flow chromatography column, patent number: 202220697921.8, entitled "A Radial and Tangential Flow Bottom for a Chromatography Column and a Combined Chromatography System." This tangential flow structure enables simultaneous tangential flow filtration and chromatographic loading, resulting in high linear flow rates and overload loading, achieving extremely high dynamic binding capacity.

[0004] Therefore, downstream biopharmaceutical technologies require different platforms for different processes to achieve the corresponding processes, thus requiring different system combinations. The disadvantage is that there are many different types of platforms, which have limitations. One platform can only meet the needs of the corresponding process and cannot be shared. Therefore, the utility model can modularize the platform to achieve different processes and reduce costs. Utility Model Content

[0005] In order to overcome the shortcomings of the above-mentioned existing technologies, the utility model provides a fully automatic multifunctional pilot platform system and its chromatography system, which can be modularized and multifunctional according to different process requirements, meet the production process of downstream biopharmaceuticals, and achieve the effect of reducing costs and increasing efficiency.

[0006] In order to achieve the above purpose, the technical solution adopted by the utility model is:

[0007] In response to the above technical problems, the first aspect of the disclosed content of the utility model proposes a fully automatic multifunctional pilot platform system, which includes a first input main pipeline with a first input pump and a second input main pipeline with a second input pump; the first input main pipeline is connected to multiple input pipelines and is controlled on and off by corresponding valves; the second input main pipeline is also respectively connected to the above-mentioned multiple input pipelines and is controlled on and off by corresponding valves; the outlet ends of the multiple input pipelines are respectively connected to the liquid inlet ports of their respective corresponding movable installation modules, and the movable installation modules are also provided with multiple reflux ports and multiple liquid outlet ports; the liquid outlet ports are respectively connected to multiple collection pipelines through output pipelines.

[0008] In some embodiments, the movable installation module can be used to install a chromatography column.

[0009] In some embodiments, there are two input pipes; two corresponding liquid inlet ports; two reflux ports; two liquid outlet ports; and two output pipes.

[0010] In some embodiments, the first input main pipeline and the second input main pipeline are connected to the two input pipelines through a first valve array group; the first valve array group includes four valves connected end to end to form a rectangular valve array; one group of opposite sides of the rectangular valve array are respectively connected to the corresponding first input main pipeline and the second input main pipeline; the other group of opposite sides of the rectangular valve array are respectively connected to the corresponding two input pipelines.

[0011] In some embodiments, there are two collecting pipes.

[0012] In some embodiments, the two output pipes are connected to the two collection pipes through a second valve array group; the second valve array group includes four valves connected end to end to form a rectangular valve array; one group of opposite sides of the rectangular valve array are respectively connected to the corresponding output pipes; the other group of opposite sides of the rectangular valve array are respectively connected to the corresponding two collection pipes.

[0013] In some embodiments, the second input main pipeline is connected to two collecting pipelines respectively through two valves.

[0014] In some embodiments, one side of the output end of the second input pump of the second input main pipeline is connected to the first input main pipeline through a valve.

[0015] The utility model discloses a second aspect proposes a kind of full-automatic multifunctional chromatography system, the chromatography system includes multiple RTF chromatographic columns, the bottom of each described RTF chromatographic column's import end is connected with corresponding liquid inlet port, the bottom of each described RTF chromatographic column's reflux end is connected with corresponding reflux port;Each described RTF chromatographic column top export end connects liquid outlet port;Each described liquid inlet port is connected with the outlet end of corresponding input pipeline, and the import end of each input pipeline is respectively connected with the first input main pipeline with first input pump, the second input main pipeline with second input pump, and it is connected by corresponding valve control on-off;Each described liquid outlet port is respectively connected with the import end of corresponding output pipeline, and the outlet end of each output pipeline is connected with multiple collection pipelines.

[0016] In some embodiments, the first input main pipeline is provided with air trapping.

[0017] In some embodiments, the first input main pipeline is provided with a mixer.

[0018] In some embodiments, the RTF chromatographic column is provided with two.

[0019] In some embodiments, the first input main pipeline and the second input main pipeline are connected with two input pipelines through a first valve array group;The first valve array group includes four valves connected head to tail, forming a rectangular valve array;One pair of opposite sides of the rectangular valve array is respectively connected with corresponding first input main pipeline and second input main pipeline;The other pair of opposite sides of the rectangular valve array is respectively connected with corresponding two input pipelines.

[0020] In some embodiments, the input pipeline and the output pipeline in each described RTF chromatographic column are communicated through a valve.

[0021] In some embodiments, the collection pipeline is provided with two.

[0022] In some embodiments, the two output pipelines are connected with two collection pipelines through a second valve array group;The second valve array group includes four valves connected head to tail, forming a rectangular valve array;One pair of opposite sides of the rectangular valve array is respectively connected with corresponding output pipeline;The other pair of opposite sides of the rectangular valve array is respectively connected with corresponding two collection pipelines.

[0023] In some embodiments, the second input main pipeline is respectively connected with two collection pipelines through two valves respectively.

[0024] In some embodiments, the import end of the second input main pipeline is connected with the first input main pipeline through a valve.

[0025] In some embodiments, the two reflux ports are respectively connected with corresponding reflux pipelines, and the reflux pipelines are respectively connected with filtering modules.

[0026] In some embodiments, the two return pipes are connected to the filter module through a third valve array group, the third valve array group comprising four valves connected end to end to form a rectangular valve array, one pair of opposite sides of the rectangular valve array being connected to the corresponding return pipes, and the other pair of opposite sides of the rectangular valve array being connected to the filter module and the waste pipe.

[0027] In some embodiments, the filter module comprises a filter input main pipe, a third input pump being arranged on the filter input main pipe, one end of the filter input main pipe being connected to the third valve array group, the other end of the filter input main pipe being connected to a fourth valve array group, the fourth valve array group comprising four valves connected end to end to form a rectangular valve array, the filter input main pipe being connected to one side of one pair of opposite sides of the rectangular valve array, and the other side of the one pair of opposite sides of the rectangular valve array being connected to the waste pipe, the other pair of opposite sides of the rectangular valve array being connected to one end of the corresponding filter, the other end of the two filters being connected to a fifth valve array group, the fifth valve array group comprising four valves connected end to end to form a rectangular valve array, the other end of the two filters being connected to one pair of opposite sides of the rectangular valve array, one side of the other pair of opposite sides of the rectangular valve array being connected to the liquid input pipe, and the other side of the other pair of opposite sides of the rectangular valve array being connected to the first input main pipe.

[0028] In some embodiments, one side of one pair of opposite sides of the fifth valve array group is connected to the liquid input pipe, and the other side of the one pair of opposite sides of the rectangular valve array is connected to the first input main pipe and the waste pipe.

[0029] The beneficial effects of the present utility model are as follows:

[0030] 1. The movable installation module can expand the platform, realize modularization, realize multifunctional according to different process requirements, and meet the production process of the downstream of biological medicine.

[0031] 2. Two tangential flow chromatography columns are used in the present utility model, which are alternately operated: one is in the loading mode, and the other is in the elution mode. By doing so, the feed can be continuously loaded into the RTF column, and there is almost no residual in the flow path and the circulation loop, and the circulation loop does not have to be stopped and restarted multiple times.

[0032] 3. The return end of the tangential flow adopts a circulation loop, which drives the circulation while loading the tangential flow chromatography column. BRIEF DESCRIPTION OF DRAWINGS

[0033] Figure 1 The figure is a structural schematic diagram of the present utility model.

[0034] Figure 2 The figure is a structural diagram of the full-automatic multifunctional chromatography system of the present utility model. DETAILED DESCRIPTION

[0035] The utility model will be described further in connection with the drawings

[0036] The technical content of the utility model is described below through specific embodiments, and other advantages and effects of the utility model can be easily understood by those skilled in the art from the disclosed content. The utility model can also be implemented or applied through other different specific embodiments. Those skilled in the art can make various modifications and changes without departing from the spirit of the utility model.

[0037] Before describing the specific embodiments of the present disclosure in detail, first, some terms used in the present disclosure are explained.

[0038] Unless otherwise defined herein, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. Reference to a technical term used herein is intended to refer to the technical term as commonly understood in the art, including variations or substitutions of the technical term that would be apparent to one of ordinary skill in the art. Although the following terms are believed to be well understood by one of ordinary skill in the art, the following definitions are set forth to better explain the present utility model. When a trade name appears herein, it is intended to refer to its corresponding product. All patents, published patent applications, and publications recited herein are incorporated by reference herein.

[0039] Unless otherwise indicated herein, a plurality of items, such as "a," "an," "the," etc., can refer to one or more than one. The term "one or more" or "at least one" can mean 1, 2, 3, 4, 5, 6, 7, 8, 9, or more.

[0040] The terms "connected," "coupled," or "coupling," or similar terms as used herein, are not limited to direct connections, but also include indirect connections.

[0041] A "sample" as described herein is a biomolecule, including a protein, a nucleic acid, a lipid, a carbohydrate, a small nucleotide, an amino acid, and derivatives thereof.

[0042] The term "on-line monitoring" or "real-time monitoring" as used herein refers to detecting certain parameters or properties of a buffer, a reaction fluid, a fluid flowing out from a flow reactor, such as pH value, pressure, flow rate, conductivity, etc., in real time during the use of a chromatography system. Unlike off-line detection or analysis, on-line monitoring or real-time monitoring can provide real-time feedback of the detection results.

[0043] The A2 Buffer stock tank, the A3 Buffer stock tank, the A4 Buffer stock tank, the A5 Buffer stock tank, the P1 stock tank, the P1-W stock tank, the P2 stock tank, the P2-W stock tank, the hot WFI stock tank, and the cold WFI stock tank as referred to herein refer to tanks for storing different solutions, and are not specific limited tanks, and any container capable of storing can be used.

[0044] The positional relationship words "up", "down", "left", "right", "front", "back" and the like as referred to herein are determined according to the layout direction of the drawings, and are only used to represent relative positional relationship. When the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0045] The RTF chromatography column as referred to herein is a chromatography column independently developed by the company and has a patent, with the patent number 202220697921.8. The RTF chromatography column has a radial and tangential flow structure, can be loaded through tangential flow at the bottom of the column, and at the same time, the upper part of the column head can be washed and eluted. The RTF chromatography column realizes high linear flow rate and overload loading through the mode of circulating loading and washing with balancing liquid, thereby realizing extremely high dynamic capacity.

[0046] The application scenario of the patent is laboratory scale and pilot scale, and the maximum flow range of a single pump is 1000 ml / min.

[0047] As Figure 1As shown, the multifunctional full-automatic pilot platform system comprises a first input main pipeline 1, the inlet end of the first input main pipeline 1 is connected with the outlets of a plurality of liquid storage tanks including A2 Buffer liquid storage tank, sample liquid storage tank; the outlets of the two liquid storage tanks are respectively provided with a first valve 106 and a second valve 104; the first input main pipeline 1 is respectively provided with a first input pump 2, a first pressure sensor 3, a first flow meter 4, a third valve 5, a mixer 6 and an air trap 7 from left to right; the inlet end of the air trap 7 is connected with the first input main pipeline 1 through a fourth valve 8, the outlet end of the air trap 7 is connected with the first input main pipeline 1 through a fifth valve 9, and the sixth valve 10 is arranged on the pipeline of the inlet end and the outlet end of the air trap 7. A second input main pipeline 11, the inlet end of the second input main pipeline 11 is connected with the outlets of a plurality of liquid storage tanks including A3 Buffer liquid storage tank, A4 Buffer liquid storage tank, A5 Buffer liquid storage tank; the outlets of the three liquid storage tanks are respectively provided with a seventh valve 102, an eighth valve 101 and a ninth valve 103; the outlet of the A3 Buffer liquid storage tank is connected with the inlet end of the first input main pipeline 1 through a tenth valve 105. The second input main pipeline 11 is respectively provided with a second input pump 12, a second pressure sensor 13, a second flow meter 14, an eleventh valve 15 and a twelfth valve 16 from left to right; the first input main pipeline 1 and the second input main pipeline 11 are connected through a thirteenth valve 17, one end of the thirteenth valve 17 is connected between the third valve 5 and the mixer 6, and the other end is connected between the second flow meter 14 and the eleventh valve 15.

[0048] A first valve array group 17, the first valve array group 17 is a rectangular valve array formed by four valves connected in series, as shown in the figure, the fourteenth valve 18, the fifteenth valve 19, the sixteenth valve 20 and the seventeenth valve 21 in the clockwise direction. The outlet end of the first input main pipeline 1 is connected between the fourteenth valve 18 and the seventeenth valve 21, and the outlet end of the twelfth valve 16 of the second input main pipeline 11 is connected between the fifteenth valve 19 and the sixteenth valve 20. The fourteenth valve 18 and the fifteenth valve 19 are connected with one end of a first input pipeline 22, the other end of the first input pipeline 22 is connected with a first liquid inlet port 24 of a movable mounting module 23; the first input pipeline 22 is provided with an eighteenth valve 25; the sixteenth valve 20 and the seventeenth valve 21 are connected with one end of a second input pipeline 26, the other end of the second input pipeline 26 is connected with a second liquid inlet port 27 of the movable mounting module 23, and the second input pipeline 26 is provided with a nineteenth valve 29.

[0049] The activity installation module 23 further comprises a first backflow port 30, a second backflow port 31, a first liquid outlet port 32 and a second liquid outlet port 33. The first liquid outlet port 32 is connected to one end of a first output pipeline 34, and the second liquid outlet port 33 is connected to one end of a second output pipeline 35. The other ends of the first output pipeline 34 and the second output pipeline 35 are respectively connected to a second valve array group 36. The second valve array group 36 is a rectangular valve array formed by four valves connected in series, as shown in the figure, the twentieth valve 37, the twenty-first valve 38, the twenty-second valve 39 and the twenty-third valve 40 in a clockwise direction. The other end of the first output pipeline 34 is connected between the twenty-first valve 38 and the twenty-second valve 39, and the other end of the second output pipeline 35 is connected between the twentieth valve 37 and the twenty-third valve 40. The first collection pipeline 41 is connected to the inlet end between the twentieth valve 37 and the twenty-first valve 38, and the second collection pipeline 42 is connected to the inlet end between the twenty-second valve 39 and the twenty-third valve 40. The first collection pipeline 41 is connected to the P1 storage tank and the P1-W storage tank through the twenty-fourth valve 43 and the twenty-fifth valve 44, respectively. The second collection pipeline 42 is connected to the P2 storage tank and the P2-W storage tank through the twenty-sixth valve 45 and the twenty-seventh valve 46, respectively. The twelfth valve 16 of the second input main pipeline 11 is connected to the first collection pipeline 41 and the second collection pipeline 42 through the first intermediate pipeline 47 and the second intermediate pipeline 48, respectively. The two ends of the first intermediate pipeline 48 are respectively provided with the twenty-eighth valve 49 and the twenty-ninth valve 50, and the two ends of the second intermediate pipeline 48 are respectively provided with the thirtieth valve 51 and the thirty-first valve 52.

[0050] The first output pipeline 34 is provided with a thirty-second valve 53, and the second output pipeline 35 is provided with a thirty-third valve 54.

[0051] The first backflow port 30 of the activity installation module 23 is connected to the inlet end of the filter module 56 through the thirty-fourth valve 55, and the second backflow port 31 of the activity installation module 23 is connected to the inlet end of the filter module 56 through the thirty-fifth valve 57. The outlet end of the filter module 56 is connected to the third valve 5 of the first input main pipeline 1 and the mixer 6 through the thirty-sixth valve 58.

[0052] Referring to Figure 2 The full-automatic multifunctional chromatography system shown in the figure is provided with two RTF chromatography columns on the above-mentioned leading platform system.

[0053] The first input main pipeline 1 is connected with the outlets of a plurality of liquid storage tanks, including A2 Buffer liquid storage tank and sample liquid storage tank; the outlets of two liquid storage tanks are respectively provided with first valve 106 and second valve 104; the first input main pipeline 1 is respectively provided with first input pump 2, first pressure sensor 3, first flow meter 4, third valve 5, mixer 6 and air trap 7 from left to right; the inlet end of the air trap 7 is connected with the first input main pipeline 1 through fourth valve 8, the outlet end of the air trap 7 is connected with the first input main pipeline 1 through fifth valve 9, and the sixth valve 10 is arranged on the pipeline of the inlet end and the outlet end of the air trap 7. The second input main pipeline 11 is connected with the outlets of a plurality of liquid storage tanks, including A3 Buffer liquid storage tank, A4 Buffer liquid storage tank and A5 Buffer liquid storage tank; the outlets of three liquid storage tanks are respectively provided with seventh valve 102, eighth valve 101 and ninth valve 103; the outlet of the A3 Buffer liquid storage tank is connected with the inlet end of the first input main pipeline 1 through tenth valve 105. The second input main pipeline 11 is respectively provided with second input pump 12, second pressure sensor 13, second flow meter 14, eleventh valve 15 and twelfth valve 16 from left to right; the first input main pipeline 1 and the second input main pipeline 11 are connected through thirteenth valve 17, one end of the thirteenth valve 17 is connected between the third valve 5 and the mixer 6, and the other end is connected between the second flow meter 14 and the eleventh valve 15.

[0054] The first valve array group 17 is a rectangular valve array formed by four valves connected in series, including fourteenth valve 18, fifteenth valve 19, sixteenth valve 20 and seventeenth valve 21 in the clockwise direction; the outlet end of the first input main pipeline 1 is connected between the fourteenth valve 18 and the seventeenth valve 21, and the outlet end of the twelfth valve 16 of the second input main pipeline 11 is connected between the fifteenth valve 19 and the sixteenth valve 20; one end of the first input pipeline 22 is connected between the fourteenth valve 18 and the fifteenth valve 19, and the other end of the first input pipeline 22 is connected with the bottom inlet of the first RTF chromatographic column 65 through the first liquid inlet port 24 of the movable mounting module 23; the eighteenth valve 25 is arranged on the first input pipeline 22; one end of the second input pipeline 26 is connected between the sixteenth valve 20 and the seventeenth valve 21, and the other end of the second input pipeline 26 is connected with the inlet end of the second RTF chromatographic column 66 through the second liquid inlet port 27 of the movable mounting module 23; the nineteenth valve 29 is arranged on the second input pipeline 26.

[0055] The active installation module 23 further comprises a first backflow port 30, a second backflow port 31, a first liquid outlet port 32 and a second liquid outlet port 33. The first RTF chromatographic column 65 is connected to one end of the first output pipeline 34 through the first liquid outlet port 32, and the second RTF chromatographic column 66 is connected to one end of the second output pipeline 35 through the second liquid outlet port 33. The other ends of the first output pipeline 34 and the second output pipeline 35 are respectively connected to the second valve array group 36. The second valve array group 36 is a rectangular valve array formed by four valves connected in series, as shown in the figure, the twentieth valve 37, the twenty-first valve 38, the twenty-second valve 39 and the twenty-third valve 40 in the clockwise direction. The other end of the first output pipeline 34 is connected between the twenty-first valve 38 and the twenty-second valve 39, and the other end of the second output pipeline 35 is connected between the twentieth valve 37 and the twenty-third valve 40. The first collection pipeline 41 is connected to the inlet end between the twentieth valve 37 and the twenty-first valve 38; the second collection pipeline 42 is connected to the inlet end between the twenty-second valve 39 and the twenty-third valve 40. The first collection pipeline 41 is respectively connected to the P1 liquid storage tank and the P1-W liquid storage tank through the twenty-fourth valve 43 and the twenty-fifth valve 44. The second collection pipeline 42 is respectively connected to the P2 liquid storage tank and the P2-W liquid storage tank through the twenty-sixth valve 45 and the twenty-seventh valve 46. The outlet of the twelfth valve 16 of the second input main pipeline 11 is respectively connected to the first collection pipeline 41 and the second collection pipeline 42 through the first intermediate pipeline 47 and the second intermediate pipeline 48. The two ends of the first intermediate pipeline 48 are respectively provided with the twenty-eighth valve 49 and the twenty-ninth valve 50, and the two ends of the second intermediate pipeline 48 are respectively provided with the thirtieth valve 51 and the thirty-first valve 52.

[0056] The first output pipeline 34 is provided with the thirty-second valve 53 and the thirty-seventh valve 59. The second output pipeline 35 is provided with the thirty-third valve 54 and the thirty-eighth valve 60. The first input pipeline 22 and the first output pipeline 34 are provided with the first connecting pipeline 61. One end of the first connecting pipeline 61 is connected between the eighteenth valve 25 and the first valve array group 17, and the other end of the first connecting pipeline 61 is connected between the thirty-second valve 53 and the thirty-seventh valve 59. The second input pipeline 26 and the second output pipeline 35 are provided with the second connecting pipeline 62. One end of the second connecting pipeline 62 is connected between the nineteenth valve 29 and the first valve array group 17, and the other end of the second connecting pipeline 62 is connected between the thirty-third valve 54 and the thirty-eighth valve 60. The first connecting pipeline 61 and the second connecting pipeline 62 are respectively provided with the thirty-ninth valve 63 and the fortieth valve 64.

[0057] The bottom reflux port of the first RTF chromatographic column 65 is connected to the third valve array group 67 through the first reflux port 30, and the bottom reflux port of the second RTF chromatographic column 66 is connected to the third valve array group 68 through the second reflux port 31. The third valve array group 67 is a rectangular valve array formed by four valves connected in a row, as shown in the figure, in a clockwise direction, the forty-first valve 69, the forty-second valve 70, the forty-third valve 71, and the forty-fourth valve 72. The bottom reflux port of the first RTF chromatographic column 65 is connected between the forty-first valve 69 and the forty-fourth valve 72, and the bottom reflux port of the second RTF chromatographic column 66 is connected between the forty-second valve 70 and the forty-third valve 71. The forty-third valve 71 and the forty-fourth valve 72 are connected to one end of the filter input main pipe 73, and the forty-first valve 69 and the forty-second valve 70 are connected to the waste pipe. The other end of the filter input main pipe 73 is connected to the fourth valve array group 74, and the filter input main pipe 73 is sequentially provided with a third input pump 75, a third flow meter 76, a third pressure sensor 77, and a forty-fifth valve 78 from right to left. The fourth valve array group 74 is a rectangular valve array formed by four valves connected in a row, as shown in the figure, in a clockwise direction, the forty-sixth valve 79, the forty-seventh valve 80, the forty-eighth valve 81, and the forty-ninth valve 82. The other end of the filter input main pipe 73 is connected between the forty-seventh valve 80 and the forty-eighth valve 81, and the forty-sixth valve 79 and the forty-ninth valve 82 are connected to the waste pipe. The bottom end of the first filter 83 is connected between the forty-sixth valve 79 and the forty-seventh valve 80, and the end of the second filter 84 is connected between the forty-eighth valve 81 and the forty-ninth valve 82. The top ends of the first filter 83 and the second filter 84 are respectively connected to the fifth valve array group 85, which is a rectangular valve array formed by four valves connected in a row, as shown in the figure, in a clockwise direction, the fiftieth valve 86, the fifty-first valve 87, the fifty-second valve 88, and the fifty-third valve 89. The top end of the first filter 83 is connected between the fiftieth valve 86 and the fifty-first valve 87, and the top end of the second filter 84 is connected between the fifty-second valve 88 and the fifty-third valve 89. The outlet of the liquid input pipe 90 is connected between the fiftieth valve 86 and the fifty-third valve 89, and the inlet of the input pipe 90 is respectively connected to the hot WFI storage tank and the cold WFI storage tank. The fifty-first valve 87 and the fifty-second valve 88 are respectively connected to the waste pipe and the first input main pipe 1 through the pipe, and the connection point is between the third valve 5 and the mixer 6. The pipe is respectively provided with a fifty-fourth valve 91 and a fifty-fifth valve 92.

[0058] The first collection pipe 41 is respectively provided with a UV sensor 93, a first conductivity sensor 94, and a first pH sensor 95. The second collection pipe 42 is respectively provided with a second conductivity sensor 96 and a second pH sensor 97.

Claims

1. Fully automatic multifunctional pilot platform system, characterized by: The platform system includes a first input main pipeline with a first input pump and a second input main pipeline with a second input pump; the first input main pipeline is connected to multiple input pipelines and is controlled to be on and off by corresponding valves; the second input main pipeline is also respectively connected to the above-mentioned multiple input pipelines and is controlled to be on and off by corresponding valves; the outlet ends of the multiple input pipelines are respectively connected to the liquid inlet ports of their corresponding movable installation modules, and the movable installation modules are also provided with multiple reflux ports and multiple liquid outlet ports; the liquid outlet ports are respectively connected to multiple collection pipelines through output pipelines.

2. The fully automatic multifunctional pilot platform system according to claim 1, characterized in that: The movable installation module can be used to install a chromatography column.

3. The fully automatic multifunctional pilot platform system according to claim 1, characterized in that: There are two input pipes; two corresponding liquid inlet ports; two reflux ports; two liquid outlet ports; and two output pipes.

4. The fully automatic multifunctional pilot platform system according to claim 3, characterized in that: The first input main pipeline and the second input main pipeline are connected to the two input pipelines through a first valve array group; the first valve array group includes four valves connected end to end to form a rectangular valve array; one group of opposite sides of the rectangular valve array are respectively connected to the corresponding first input main pipeline and the second input main pipeline; the other group of opposite sides of the rectangular valve array are respectively connected to the corresponding two input pipelines.

5. The fully automatic multifunctional pilot platform system according to claim 1 or 4, characterized in that: There are two collecting pipes.

6. The fully automatic multifunctional pilot platform system according to claim 5, characterized in that: The two output pipes are connected to the two collection pipes through a second valve array group; the second valve array group includes four valves connected end to end to form a rectangular valve array; one group of opposite sides of the rectangular valve array is respectively connected to the corresponding output pipes; the other group of opposite sides of the rectangular valve array is respectively connected to the corresponding two collection pipes.

7. The fully automatic multifunctional pilot platform system according to claim 5, characterized in that: The outlet end of the second input main pipeline is connected to two collecting pipelines through two valves respectively.

8. The fully automatic multifunctional pilot platform system according to claim 5, characterized in that: One side of the output end of the second input pump of the second input main pipeline is connected to the first input main pipeline through a valve.

9. A fully automatic multifunctional chromatography system based on any one of claims 1 to 8, characterized in that: The chromatography system includes multiple RTF chromatography columns, the inlet end at the bottom of each RTF chromatography column is connected to a corresponding liquid inlet port, and the reflux end at the bottom of each RTF chromatography column is connected to a corresponding reflux port; the outlet end at the top of each RTF chromatography column is connected to a liquid outlet port; each of the liquid inlet ports is connected to the outlet end of a corresponding input pipeline, and the inlet end of each input pipeline is respectively connected to a first input main pipeline with a first input pump and a second input main pipeline with a second input pump, and the on-off is controlled by a corresponding valve; each of the liquid outlet ports is respectively connected to the inlet end of a corresponding output pipeline, and the outlet end of each output pipeline is connected to multiple collection pipelines.

10. The fully automatic multifunctional chromatography system according to claim 9, characterized in that: An air trap is provided on the first input main pipeline.

11. The fully automatic multifunctional chromatography system according to claim 9, characterized in that: The first input main pipeline is provided with a mixer.

12. The fully automatic multifunctional chromatography system according to claim 9, characterized in that: There are two RTF chromatography columns.

13. The fully automatic multifunctional chromatography system according to claim 12, characterized in that: The first input main pipeline and the second input main pipeline are connected to the two input pipelines through a first valve array group; the first valve array group includes four valves connected end to end to form a rectangular valve array; one group of opposite sides of the rectangular valve array are respectively connected to the corresponding first input main pipeline and the second input main pipeline; the other group of opposite sides of the rectangular valve array are respectively connected to the corresponding two input pipelines.

14. The fully automatic multifunctional chromatography system according to claim 12, characterized in that: The input pipeline and the output pipeline in each RTF chromatography column are connected via a valve.

15. The fully automatic multifunctional chromatography system according to claim 9, characterized in that: There are two collecting pipes.

16. The fully automatic multifunctional chromatography system according to claim 15, characterized in that: The two output pipes are connected to the two collection pipes through a second valve array group; the second valve array group includes four valves connected end to end to form a rectangular valve array; one group of opposite sides of the rectangular valve array is respectively connected to the corresponding output pipes; the other group of opposite sides of the rectangular valve array is respectively connected to the corresponding two collection pipes.

17. The fully automatic multifunctional chromatography system according to claim 16, characterized in that: The second input main pipeline is connected to the two collecting pipelines respectively through two valves.

18. The fully automatic multifunctional chromatography system according to claim 9, characterized in that: The inlet end of the second input main pipeline is connected to the first input main pipeline through a valve.

19. The fully automatic multifunctional chromatography system according to claim 9, characterized in that: The two reflux ports of the RTF chromatography column are respectively connected to corresponding reflux pipes, and the reflux pipes are respectively connected to the filtration modules.

20. The fully automatic multifunctional chromatography system according to claim 19, characterized in that: The two return pipes are connected to the filter module through a third valve array group. The third valve array group includes four valves connected end to end to form a rectangular valve array; one group of opposite sides of the rectangular valve array is respectively connected to the corresponding return pipes; one side of the other group of opposite sides of the rectangular valve array is connected to the filter module, and the other side is connected to the waste pipe.

21. The fully automatic multifunctional chromatography system according to claim 20, characterized in that: The filtration module includes a filtration input main pipeline, on which a third input pump is provided, one end of the filtration input main pipeline is connected to the third valve array group, and the other end of the filtration input main pipeline is connected to the fourth valve array group, the fourth valve array includes four valves connected end to end to form a rectangular valve array, the filtration input main pipeline is connected to one side of a group of opposite sides of the rectangular valve array, wherein the other side is connected to the waste discharge pipeline; the other side of the rectangular valve array is respectively connected to one end of the corresponding filter; the other ends of the two filters are respectively connected to the fifth valve array group; the fifth valve array group includes four valves connected end to end to form a rectangular valve array; the other ends of the two filters are respectively connected to a group of opposite sides of the rectangular valve array; one side of the other group of opposite sides of the rectangular valve array is connected to the liquid input pipeline, and the other side of the other group of opposite sides of the rectangular valve array is connected to the first input main pipeline.

22. The fully automatic multifunctional chromatography system according to claim 21, characterized in that: One side of one group of opposite sides of the fifth valve array group is connected to the liquid input pipeline, and the other side of one group of opposite sides of the fifth valve array group is connected to the first input main pipeline and the waste discharge pipeline.

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