Device for testing listening function of zebra fish

Abstract

The utility model provides a zebra fish listening function testing device which comprises a box body, a light source assembly, an observation assembly and an image acquisition assembly, the light source assembly, the observation assembly and the image acquisition assembly are arranged in the box body from bottom to top, and the observation assembly comprises a culture solution container and a zebra fish observation container; the culture solution container is arranged above the light source assembly, and the bottom of the culture solution container is of a light-permeable structure; the observation container is arranged in the culture solution container and is opposite to the bottom of the culture solution container at an interval; the permeable structure is used for limiting passing of zebra fish and allowing the culture solution in the culture solution container to pass; a sound production assembly is also arranged in the culture solution container; the image acquisition assembly is used for acquiring motion information of the zebra fish in the observation container. The observation assembly of the double-layer structure can replace experiment objects or increase or decrease water levels at any time, and the flexibility of the experiment device is improved. The test device can realize research on the influence of sound stimulation on the circadian rhythm; and the image acquisition assembly automatically acquires zebra fish image information, so that the accuracy of the testing device is improved.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the field of behavior response experiment device, especially relates to a zebra fish hearing function test device. BACKGROUND

[0002] Zebra fish has become one of the core model organisms in modern biomedical research due to its rapid development, transparent embryo, easy-to-edit genes, high similarity to human genes, and low cost and high throughput advantages. Zebra fish has an inner ear-lateral line dual system hearing mechanism, relies on low-frequency sensitive (100-400 Hz) hair cells and renewable otolith structure, and is an ideal model for studying hearing impairment repair and genetic deafness. When conducting experimental research, zebra fish can be placed in a hearing function test device, and after providing sound stimulation, the hearing function of zebra fish can be tested by observing the movement of zebra fish.

[0003] Patent CN204500712U discloses a hearing function test device, which comprises a loudspeaker water tank and an audiometry water tank, a sound transmission hole slot is formed on the baffle adjacent to the loudspeaker water tank and the audiometry water tank, an underwater loudspeaker is arranged in the loudspeaker water tank, a zebra fish placement support and a zebra fish fixing device for fixing zebra fish and test electrodes are installed in the audiometry water tank, the surfaces of the loudspeaker water tank and the audiometry water tank are grounded to conduct static electricity on the surface of the water tank, thereby improving the experimental conditions of underwater sound stimulation and underwater recording, and the loudspeaker water tank can generate stable high-intensity noise for the study of noise-induced hearing loss in zebra fish. However, the hearing function test device only considers anti-interference sound stimulation, and cannot collect the changes in the behavior state of zebra fish under different sound wave tests, nor can it study the influence of sound stimulation on the circadian rhythm of zebra fish. Therefore, the test device has deficiencies in accuracy and flexibility, and cannot meet the high standard requirements of modern scientific research. UTILITY MODEL CONTENT

[0004] The utility model aims to solve the problem of the hearing function test device in the prior art in terms of accuracy and flexibility.

[0005] To solve the above technical problems, the embodiment of the utility model discloses a zebra fish hearing function's testing arrangement, include: box, the box is formed with the closed containing space around, and the material quality of box adopts the material quality of not passing through the light, light source subassembly, light source subassembly sets up in the containing space of box, observation subassembly, observation subassembly sets up in the containing space of box, and including culture solution container and zebra fish observation container, in the height direction of box, culture solution container sets up in the upper of light source subassembly, and culture solution container sets up as the structure of the bottom light -transmitting, observation container sets up in culture solution container, and with the bottom relative interval of culture solution container Set up, observation container includes observation board, fixed plate and water -permeable structure, the material quality of observation board and fixed plate all are the material quality of light -transmitting, the observation board is formed with a plurality of parallelly arranged observation grooves, every observation groove extends along the height direction of observation container, and every observation groove's upper end and lower end all open, water -permeable structure is used for limiting zebra fish and allowing culture solution in culture solution container to pass through, water -permeable structure includes microporous filter membrane, microporous filter membrane sets up in every observation groove's lower end, observation board, microporous filter membrane and fixed plate are sequentially stacked and fixedly connected along the height direction of observation container from top to bottom, and fixed plate is provided with a plurality of through -holes corresponding to the opening of the lower end of a plurality of observation grooves to make culture solution in culture solution container can flow into the corresponding observation groove from each through -hole through microporous filter membrane, sound -emitting subassembly, sound -emitting subassembly sets up in culture solution container, image acquisition subassembly, image acquisition subassembly sets up in the top of box, and with the upper portion of observation container is opposite, for gathering the movement information of zebra fish in observation container.

[0006] Adopt the above technical scheme, set up the observation subassembly of the double -deck structure of culture solution container of lower layer, observation container of upper layer, make observation container can replace experimental object or increase and decrease the water level at any time, improved the flexibility of experimental device. The testing device also includes a light source assembly that can provide light conditions and a sound-emitting assembly that can provide sound conditions. During testing, the sound-emitting assembly emits sound in the water container, which transmits mechanical vibration through the elastic collision of water molecules in the water, achieving sound stimulation of the zebra fish in the observation container. By adjusting the light source assembly to simulate day and night, the influence of sound stimulation on the circadian rhythm can be studied. The image acquisition assembly automatically collects zebra fish image information during the test process and can transmit it to a computer for analysis, improving the accuracy of the testing device.

[0007] Moreover, the observation container has a simple and stable overall structure and good light transmission. The multiple observation grooves facilitate the observation of zebra fish in different observation grooves, and the behavior trajectories of zebra fish in different observation grooves can be compared.

[0008] According to another specific embodiment of the utility model, the pore size of the microporous filter membrane is 50 μm -200 μm.

[0009] The above technical solution can ensure sufficient exchange of culture solution inside and outside each observation tank and effectively maintain the relative independence of each observation tank.

[0010] According to another specific embodiment of the present application, the zebrafish hearing function testing device disclosed by the embodiment of the present application is characterized in that the inner walls of the opposite two side walls of the culture solution container are respectively provided with clamping pieces, the observation plate is provided with clamped pieces at positions corresponding to the clamping pieces, and the clamping pieces and the corresponding clamped pieces are clamped and fixed, so that the observation container and the culture solution container are detachably fixedly connected.

[0011] The above technical solution can ensure the stability of the observation assembly structure by clamping, and facilitate water replacement or replacement of experimental objects after the observation container is taken out.

[0012] According to another specific embodiment of the present application, the zebrafish hearing function testing device disclosed by the embodiment of the present application is characterized in that the observation assembly further comprises a support, the support is arranged in the culture solution container, and the upper end of the support is fixedly connected with the observation container, and the lower end of the support is supported on the bottom of the culture solution container.

[0013] The above technical solution can ensure the stability of the observation assembly structure by clamping, and facilitate water replacement or replacement of experimental objects after the observation container is taken out.

[0014] According to another specific embodiment of the present application, the zebrafish hearing function testing device disclosed by the embodiment of the present application is characterized in that the sound emitting assembly comprises a plurality of loudspeakers, and the plurality of loudspeakers are distributed at one end of the fixed plate away from the observation plate and are fixedly connected with the fixed plate.

[0015] The above technical solution can ensure the stability of the observation assembly structure by clamping, and facilitate water replacement or replacement of experimental objects after the observation container is taken out.

[0016] According to another specific embodiment of the present application, the zebrafish hearing function testing device disclosed by the embodiment of the present application is characterized in that the peripheral wall of the culture solution container is composed of the peripheral wall of the middle part of the box in the height direction, and the middle part of the box is detachably connected with the upper part and the lower part of the box, the upper part of the box comprises the top of the box, and the lower part of the box comprises the bottom of the box.

[0017] The above technical solution makes the experimental device compact in structure and facilitates dismounting of the observation assembly and the light source assembly.

[0018] According to another specific embodiment of the present application, the zebrafish hearing function testing device disclosed by the embodiment of the present application is characterized in that the light source assembly comprises a white light source group and a red light source group connected in parallel, the white light source group comprises a plurality of white light LEDs connected in series, the red light source group comprises a plurality of red light LEDs connected in series, and the bottom of the culture solution container is a light homogenizing plate.

[0019] The above technical solution can realize independent control of two groups of different light sources to meet the light illumination requirements under different experimental conditions.

[0020] According to another specific embodiment of the utility model, the utility model discloses the test device of zebra fish hearing function, and the image acquisition component includes near-infrared camera;The top of the box is provided with the mounting hole, and the near-infrared camera is fixedly arranged in the mounting hole, and the optical center of the near-infrared camera and the connecting line of the geometric center of the observation container extend along the height direction of the box.

[0021] The above technical solution can improve the clarity and contrast of underwater zebra fish body imaging. BRIEF DESCRIPTION OF DRAWINGS

[0022] Figure 1 It is the structure schematic diagram of the test device of zebra fish hearing function of the utility model (wherein in order to facilitate the internal structure of the box, one side is removed);

[0023] Figure 2 It is the structure schematic diagram of the observation component of the test device of zebra fish hearing function of the utility model (wherein in order to facilitate the internal structure of the culture solution container, one side is removed);

[0024] Figure 3 It is the split structure schematic diagram of the observation component of Figure 2 ;

[0025] Figure 4 It is the structure schematic diagram of the observation container of the observation component of Figure 2 ;

[0026] Figure 5 It is the structure schematic diagram of another embodiment of the observation component of the test device of zebra fish hearing function of the utility model (wherein in order to facilitate the internal structure of the culture solution container, one side is removed);

[0027] Figure 6 It is the photo of multiple observation grooves (bowl shape) of the observation container of the test device of zebra fish hearing function of the utility model;

[0028] Figure 7 It is the motion trajectory example drawing of zebra fish in an observation groove obtained in the experimental process of the test device of zebra fish hearing function of the utility model.

[0029] BRIEF DESCRIPTION OF DRAWINGS

[0030] 1, box, 11, containing space, 2, light source assembly, 3, observation assembly, 31, culture solution container, 311, bottom, 312, peripheral wall, 313, clamping piece, 32, observation container, 321, water permeable structure, 322, observation plate, 3221, observation groove, 323, fixing plate, 3231, through hole, 324, clamped piece, 33, support, 4, sound production assembly, 41, loudspeaker, 5, image acquisition assembly. DETAILED DESCRIPTION

[0031] In order to more clearly understand the test device for zebrafish hearing function and the setting mode thereof provided by the present application, the test device for zebrafish hearing function will be described in detail below in combination with the drawings.

[0032] The test device for zebrafish hearing function provided by the present application, as shown in Figures 1-5 , comprises a box 1, a light source assembly 2, an observation assembly 3, a sound production assembly 4 and an image acquisition assembly 5, and each assembly will be described in detail below.

[0033] As shown in Figure 1 , the box 1 surrounds a closed containing space 11, and the material of the box 1 is light-tight material to ensure that the box 1 is not affected by external light during the experiment. Specifically, the box 1 provides a relatively closed space for the test device and provides support; the shape of the box 1 can be set as needed, for example, it can be a cube (as shown in Figure 1 ), a cylinder or the like; the material of the box 1 can be wood, metal, plastic or the like, as long as it can provide stable support and is light-tight. For easy operation, one side of the box 1 can be provided in the form of a door, and the box 1 can also be provided in a detachable structure, such as detachable from the top, or divided into two or more parts connected detachably along the height direction of the box 1.

[0034] As shown in Figure 1 , the light source assembly 2 is arranged in the containing space 11 of the box 1, and specifically, the light source assembly 2 is arranged at the bottom position of the containing space 11; and emits light upward, and the light source assembly 2 is used to provide light conditions during the experiment of the test device, and the switch of the light source assembly 2 is adjusted to simulate day and night. The light source assembly 2 should include a lamp, which can be an LED lamp, an incandescent lamp, a tungsten lamp or the like.

[0035] In one embodiment, the light source assembly 2 includes a white light source group and a red light source group (not shown) connected in parallel. The white light source group includes multiple white LEDs connected in series, and the red light source group includes multiple red LEDs connected in series. The light-emitting surfaces of both the white and red light source groups face upward. Specifically, a dual-module design, with the white and red light source groups connected in parallel, is employed. Each of the white and red light source groups is equipped with independent switching power cords, enabling independent control of the two groups. The light source can also be configured to support timed switching to meet the illumination time requirements under different experimental conditions.

[0036] Observation component 3 is a component for culturing zebrafish, such as Figure 1 As shown, the observation component 3 is arranged in the accommodating space 11 of the housing 1, and includes a culture liquid container 31 for accommodating a culture liquid (specifically, water) and an observation container 32 for accommodating zebrafish. In the height direction of the housing 1, the culture liquid container 31 is arranged above the light source component 2, and the culture liquid container 31 is configured as a light-transmitting structure at the bottom 311, so that the light emitted by the light source component 2 can pass through the culture liquid container 31 and enter the observation container 32. The bottom 311 of the culture liquid container 31 can specifically be a glass plate or a transparent plastic plate. Furthermore, the other parts of the culture liquid container 31 can be made of the same material as the bottom 311, or can be made of different materials, and the bottom 311 and other parts are connected to form a closed and watertight container. In one specific embodiment, the bottom 311 of the culture liquid container 31 is a light-dispersing plate, so that the light entering the observation component 3 is evenly distributed. In one specific embodiment, as Figure 1 As shown, the peripheral wall 312 of the culture liquid container 31 is composed of the peripheral wall of the middle part of the box body 1 along the height direction, and the middle part of the box body 1 is detachably connected to the upper and lower parts of the box body 1 respectively, the upper part of the box body 1 includes the top of the box body 1, and the lower part of the box body 1 includes the bottom of the box body 1; that is, the lower end of the peripheral wall 312 of the culture liquid container 31 is detachably connected to the part of the box body 1 where the light source assembly 2 is set, and the upper end of the peripheral wall 312 of the culture liquid container 31 is detachably connected to the upper part of the box body 1; the detachable connection method can be snap connection, screw connection, etc.

[0037] like Figures 1-3 and Figure 5 As shown, observation container 32 is disposed within culture medium container 31 and is spaced relative to the bottom of culture medium container 31. That is, observation container 32 is disposed relatively above culture medium container 31, with a distance between the bottom of observation container 32 and bottom 311 of culture medium container 31. Observation container 32 includes a permeable structure 321, which is used to restrict the passage of zebrafish while allowing the culture medium in culture medium container 31 to pass through. This allows the culture medium in culture medium container 31 to freely enter observation container 32, confining the zebrafish within observation container 32 for collecting experimental data.

[0038] Specifically, the observation container 32 is used to accommodate zebrafish, so it should have a space in which the zebrafish can swim, and specifically can be set as a whole space; in order to facilitate separate observation, in one specific embodiment, as shown in FIG. Figures 1-5 As shown, the observation container 32 is provided with a plurality of observation slots 3221 (zebrafish swimming space) arranged in parallel, each observation slot 3221 extending along the height direction of the observation container 32 and having an upper end open. Specifically, the shape of the internal space of the observation slot 3221 can be bowl-shaped ( Figure 6 The observation tank 3221 may be formed by a plurality of corresponding structures such as a bowl body, a cylinder body, and a box body. The observation tank 3221 may not have an opening at the lower end. The water-permeable structure 321 may be a hole or a gap smaller than the size of the zebrafish opened on the outer wall of the observation container 32. The observation tank 3221 may also be provided with an opening at the lower end, that is, the lower end of the observation tank 3221 in the height direction is connected to the outside world. The water-permeable structure 321 may be a net or a filter membrane fixed at the lower end opening of the observation tank 3221 by a fixing belt or the like. The observation tank 3221 may also be a tank directly opened on an integral structure. In one specific embodiment, such as Figures 1-5 As shown, the observation container 32 includes an observation plate 322, which is made of a light-transmitting material, such as a transparent frosted plate; a plurality of observation slots 3221 are formed on the observation plate 322, that is, a plurality of observation slots 3221 are opened on the observation plate 322; further, each observation slot 3221 is open at the upper and lower ends along the height direction of the culture medium container 31, that is, the upper and lower ends of the observation slot 3221 are connected to the outside world; the permeable structure 321 can be specifically a net, filter membrane, etc. with a hollow size smaller than that of zebrafish, which is arranged at the opening position at the lower end of the observation container 32. In one specific embodiment, the permeable structure 321 is a microporous filter membrane, which is arranged at the lower end of each observation tank 3221; further, the pore size of the microporous filter membrane is 50μm-200μm, preferably 100μm-150μm, which ensures that the culture medium (such as water) inside and outside each observation tank 3221 is fully exchanged, and can effectively maintain the relative independence of each observation tank 3221, that is, one or more zebrafish are tested in each observation tank 3221, and the behavioral trajectories of the zebrafish in different observation tanks 3221 can be compared.

[0039] In one embodiment, Figures 1-5As shown, the observation container 32 includes an observation plate 322 and a fixing plate 323. The material of the fixing plate 323 is a light-transmitting material, such as a transparent frosted plate. Furthermore, the lower end of each observation slot 3221 is flush with the bottom surface of the observation plate 322. The water-permeable structure 321 (microporous filter membrane) is cut to a size that matches the size of the fixing plate 323. The observation plate 322, the water-permeable structure 321 (microporous filter membrane) and the fixing plate 323 are stacked and fixedly connected from top to bottom along the height direction of the observation container 32, that is, the water-permeable structure 321 (microporous filter membrane) is sandwiched between the observation plate 322 and the fixing plate 323, and the outer edge of the water-permeable structure 321 (microporous filter membrane) is aligned with the outer edge of the fixing plate 323. And, as shown in FIG. Figure 4 As shown, the fixed plate 323 is provided with a plurality of through-holes 3231 corresponding one-to-one with the openings at the lower ends of the plurality of observation slots 3221. This allows the culture fluid in the culture fluid container 31 to flow from each through-hole through the permeable structure 321 (microporous filter membrane) into the corresponding observation slot 3221. Furthermore, the observation plate 322, the permeable structure 321 (microporous filter membrane), and the fixed plate 323 can be fixedly connected by screwing, snapping, bonding, or pinning. Preferably, a screw connection is used, i.e., multiple screws are used to secure the fixed plate 323 to the observation plate 322.

[0040] Regarding the arrangement of the observation container 32 in the culture medium container 31. In one embodiment, as Figures 2-4 As shown, the inner wall surfaces of the two opposite side walls of the culture liquid container 31 are respectively provided with clamping parts 313, and the positions of the observation plate 322 corresponding to the clamping parts 313 are provided with clamped parts 324. Each clamping part 313 is clamped and fixed with the corresponding clamped part 324, so that the observation container 32 and the culture liquid container 31 are detachably fixedly connected; further, after the fixed connection, the outer periphery of the observation plate 322 is precisely connected with the inner edge of the lower culture liquid container 31. Specifically, as Figures 3-4 As shown, the engaging member 313 can be a block, and the engaged member 324 can be a connector with a matching slot structure. Of course, the engaging member 313 and the engaged member 324 can also be other existing matching engaging structures. The number of matching structures of the engaging member 313 and the engaged member 324 can be one set per wall, or multiple sets per wall (two sets per wall are shown in the figure). The specific number depends on the size and weight of the culture fluid container 31, as well as the characteristics of the engaging structure, as long as the observation container 32 is stably engaged with the culture fluid container 31.

[0041] In another specific embodiment, Figure 5As shown, the observation assembly 3 further comprises a support 33 arranged in the culture solution container 31, and the upper end of the support 33 is fixedly connected with the observation container 32, and the lower end of the support 33 is supported on the bottom 311 of the culture solution container 31. The support 33 can be specifically a supporting leg arranged at four corners of the observation plate 322, and the bottom end of the supporting leg is supported on the bottom of the observation container 32.

[0042] The above arrangement not only ensures the stability of the structure of the observation assembly 3, but also realizes the effective separation of the plurality of observation grooves 3221; and the observation container 32 can be replaced with experimental objects or the water level can be increased or decreased at any time. In use, after water is injected into the culture solution container 31, the observation container 32 is fixed to the inner wall of the culture solution container 31 through the clamping member 313 and the clamped member 324, or the observation container 32 is fixed in the culture solution container 31 through the support 33; after the test is completed, the observation container 32 can be taken out as a whole from the culture solution container 31, and further can be taken out from the box body 1, thereby facilitating the discharge of the culture solution in the culture solution container 31.

[0043] The sound generating assembly 4 is arranged in the culture solution container 31; the sound generating assembly 4 can be specifically a loudspeaker, a microphone, a buzzer, etc.; and can be arranged at the bottom, the side of the culture solution container 31, or the outside of the observation container 32, etc. When the test device performs the experiment, the sound generating assembly 4 is used to provide sound conditions, and the sound generating assembly 4 emits sound in the culture solution container 31, and the mechanical vibration is transmitted through the elastic collision of water molecules in the water, so as to realize the sound stimulation of the zebrafish in the observation container 32.

[0044] In one specific embodiment, as shown in Figure 4 The sound generating assembly 4 comprises a plurality of loudspeakers 41, the plurality of loudspeakers 41 are distributed at one end of the fixed plate 323 away from the observation plate 322 and are fixedly connected with the fixed plate 323, and the loudspeaker 41 has a loudspeaker face downward; specifically, the fixedly connected manner of the loudspeaker 41 can be adhesion, or screwing on the fixed plate 323 through other fixing auxiliary devices, etc.; preferably, each loudspeaker 41 is integrally sealed and adhered to the fixed plate 323 by using silica gel. Further, the input interface of the loudspeaker 41 is connected with a computer or other test control device through a hard line (such as a USB line), or the loudspeaker 41 can be connected with the test control device through a wireless mode such as Bluetooth.

[0045] As shown in Figure 1 The image acquisition assembly 5 is arranged at the top of the box body 1 and opposite to the upper part of the observation container 32, and is used to acquire the motion information of the zebrafish in the observation container 32. The image acquisition assembly 5 can specifically comprise a camera, a camera, or other devices that can acquire images, and the image acquisition assembly 5 is further connected with the test control device, and the test process automatically acquires the image information of the zebrafish through the image acquisition assembly 5 and can transmit the image information to the test control device for analysis, thereby improving the accuracy of the test device.

[0046] In one specific embodiment, the image acquisition component 5 comprises a near-infrared camera; the top of the box 1 is provided with a mounting hole, and the near-infrared camera is fixedly arranged in the mounting hole, and the line connecting the optical center of the near-infrared camera and the geometric center of the observation container 32 extends along the height direction of the box 1, so that the near-infrared camera is installed on the box 1 in a vertical mounting manner.

[0047] It should be particularly pointed out that the near-infrared camera specially adds an independent infrared imaging channel on the basis of standard RGB three-channel imaging, and significantly improves the definition and contrast of the zebrafish body imaging underwater through a multispectral fusion technology.

[0048] The test device for zebrafish hearing function provided by the utility model, when testing, water or other culture solution is injected into the culture solution container 31 under the observation component 3, so that the sound emitting component 4 contacts or is above the water surface, and the water therein enters the observation container 32 through the water permeable structure 321; the zebrafish larvae are placed in the observation container 32; the sound emitting component 4 emits sound in the water container, forms sound stimulation to the zebrafish in the observation container 32, and adds light illumination conditions by adjusting the light source component 2; the test process automatically collects zebrafish image information by the image acquisition component 5 and can be transmitted to a computer for analysis.

[0049] After the collected image information is transmitted to an experimental control device (such as a computer), the computer vision processing flow based on OpenCV can be used for analysis, and the test results are obtained according to the movement trajectory of the zebrafish in each observation groove. Figure 7 An example diagram of the movement trajectory of the zebrafish in one observation groove is shown. The computer vision processing flow can select an edge detection algorithm based on a Canny operator, including the following steps:

[0050] S1: image preprocessing step: a background modeling algorithm based on K-Nearest Neighbors (K-Nearest Neighbors) is used for background subtraction, and then image binarization processing is performed; a Gaussian filter algorithm and an expansion and corrosion algorithm are used to smooth the input image to reduce image noise interference.

[0051] S2: gradient calculation step: the gradient amplitude and gradient direction of various pixel points in the image are calculated by a Canny operator to establish an image gradient field.

[0052] S3: edge thinning step: a non-maximum suppression algorithm is used to process the gradient amplitude matrix, and the local maximum value point in the gradient direction is retained to realize edge thinning.

[0053] S4: Edge determination step: set high and low double threshold to distinguish edge pixels, wherein the pixel points higher than the high threshold are determined as strong edges, and the pixel points between the high and low thresholds are determined as weak edges, and complete edges are formed through edge connection processing.

[0054] S5: Select the largest connected domain, and output the center coordinate value.

[0055] It should be noted that in addition to the specific embodiments described above, other advantages and effects of the present application can be easily understood by those skilled in the art from the content disclosed in the present application. Although the description of the present application is introduced in combination with the preferred embodiments, this does not mean that the features of the present application are limited to the implementation. On the contrary, the purpose of introducing the present application in combination with the implementation is to cover other options or modifications that can be extended based on the claims of the present application. In order to provide a deep understanding of the present application, many specific details are included in the above description, and the present application can also be implemented without using these details. In addition, in order to avoid confusion or obscure the focus of the present application, some specific details will be omitted in the description. It should be noted that the embodiments in the present application and the features in the embodiments can be combined with each other without conflict.

[0056] It should be noted that in the present application, similar reference numbers and letters in the following drawings refer to similar items, so once an item is defined in one drawing, it does not need to be further defined and explained in subsequent drawings.

[0057] In the description of the present embodiment, it should be noted that the terms "upper", "lower", "inner", "bottom" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship commonly used when the product of the present application is used, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the present application.

[0058] In the description of the present embodiment, it should be noted that unless otherwise explicitly specified and limited, the terms "set", "connected", "connected" should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium, or it can be the communication inside two elements. For those skilled in the art, the specific meaning of the above terms in the present embodiment can be understood according to the specific circumstances.

[0059] Although the utility model has been illustrated and described by referring to certain preferred embodiments of the utility model, it should be understood by those skilled in the art that the above content is the further detailed description of the utility model combined with the specific embodiments, and the specific implementation of the utility model cannot be limited to these descriptions. Those skilled in the art can make various changes in form and details, including making several simple inferences or replacements, without departing from the spirit and scope of the utility model.

Claims

1. A device for testing the hearing and movement function of zebrafish, characterized in that: include: A box body, wherein the box body forms a closed accommodation space and is made of an opaque material; a light source assembly, the light source assembly being disposed in the accommodation space of the box; An observation assembly is disposed in the accommodating space of the housing and includes a culture fluid container and a zebrafish observation container; in the height direction of the housing, the culture fluid container is disposed above the light source assembly, and the culture fluid container is configured to have a light-permeable bottom; the observation container is disposed in the culture fluid container and is spaced relative to the bottom of the culture fluid container; The observation container includes an observation plate, a fixing plate, and a water-permeable structure; the observation plate and the fixing plate are both made of a light-transmitting material; a plurality of observation slots arranged in parallel are formed on the observation plate, each of the observation slots extending along the height direction of the observation container, and each of the observation slots is open at both the upper and lower ends; the water-permeable structure is used to restrict the passage of zebrafish and allow the culture medium in the culture medium container to pass through, and the water-permeable structure includes a microporous filter membrane, which is arranged at the lower end of each of the observation slots; The observation plate, the microporous filter membrane, and the fixed plate are stacked in sequence from top to bottom along the height direction of the observation container and are fixedly connected; and the fixed plate is provided with a plurality of through holes corresponding one-to-one to the openings at the lower ends of the plurality of observation slots, so that the culture fluid in the culture fluid container can flow from each through hole through the microporous filter membrane into the corresponding observation slot; a sound-generating component, the sound-generating component being disposed in the culture fluid container; An image acquisition component is arranged on the top of the box and opposite to the upper part of the observation container, and is used to collect movement information of the zebrafish in the observation container.

2. The zebrafish hearing and motor function testing device according to claim 1, wherein: The pore size of the microporous filter membrane is 50 μm-200 μm.

3. The zebrafish hearing and motor function testing device according to claim 1, wherein: The inner wall surfaces of the two opposite side walls of the culture fluid container are respectively provided with clamping parts, and the positions corresponding to the clamping parts in the observation plate are provided with clamped parts. Each clamping part is clamped and fixed with the corresponding clamped part, so that the observation container and the culture fluid container are detachably fixedly connected.

4. The zebrafish hearing and motor function testing device according to claim 1, wherein: The observation assembly further includes a bracket, which is disposed in the culture fluid container. The upper end of the bracket is fixedly connected to the observation container, and the lower end of the bracket is supported on the bottom of the culture fluid container.

5. The zebrafish hearing and motor function testing device according to claim 1, wherein: The sound-generating component includes a plurality of speakers, which are distributed at one end of the fixing plate away from the observation plate and fixedly connected to the fixing plate.

6. The zebrafish hearing and motor function testing device according to claim 1, wherein: The peripheral wall of the culture liquid container is composed of the peripheral wall of the middle part of the box body along the height direction, and the middle part of the box body is detachably connected to the upper and lower parts of the box body respectively, the upper part of the box body includes the top of the box body, and the lower part of the box body includes the bottom of the box body.

7. The device for testing the zebrafish hearing and motor function according to any one of claims 1 to 6, wherein: The light source assembly includes a white light source group and a red light source group connected in parallel; the white light source group includes a plurality of white light LEDs connected in series, and the red light source group includes a plurality of red light LEDs connected in series; And the bottom of the culture liquid container is a light-diffusing plate.

8. The device for testing the zebrafish hearing and motor function according to any one of claims 1 to 6, wherein: The image acquisition component includes a near-infrared camera; a mounting hole is provided on the top of the box, the near-infrared camera is fixedly arranged in the mounting hole, and a line connecting the optical center of the near-infrared camera and the geometric center of the observation container extends along the height direction of the box.

Images