Resuspension cavity tube based on air pressure change and bio-ink resuspension nozzle device

Abstract

The utility model relates to the technical field of biology, and discloses a resuspension cavity tube and a bio-ink resuspension nozzle device based on air pressure change, the resuspension cavity tube comprises a resuspension end and a fixed end, the resuspension end comprises a central column and a peripheral wall arranged on the outer side of the central column, and the fixed end is arranged on the central column. And a uniform mixing cavity for uniformly mixing liquid is arranged between the central column and the peripheral wall. The bio-ink re-suspension nozzle device comprises a re-suspension cavity tube, a needle tube assembly, a printing assembly and a temperature control device, the re-suspension cavity tube is arranged in the needle tube assembly so that the bio-ink can be evenly mixed, the needle tube assembly is connected with the temperature control device so that the temperature of bio-ink can be controlled, and the printing assembly is communicated with the interior of a cavity of the suspension tube. The device is used for controlling outflow and blocking of liquid. The biological ink mixing device has the technical effect that the cell concentration is more uniform in the biological ink mixing process.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the field of biotechnology, specifically relates to a resuspension cavity pipe based on air pressure change and biological ink resuspension nozzle device. BACKGROUND

[0002] Biological 3D printing produces artificial analogues, tissues and organs through layer-by-layer stacking of biological ink. Biological ink, as an important element in 3D biological printing, is usually composed of biological materials and active cells. During the biological printing process, due to the effect of gravity, when the gravity experienced by the cells is greater than the buoyancy experienced by the cells, the cells will settle and accumulate at the bottom of the biological ink container. This will cause printing structure precision, printing process blockage and subsequent culture, and the cell content of the biological ink before and after printing is greatly different, affecting the survival rate of single cells and the functional expression of tissues.

[0003] In order to solve the problem of cell settlement, researchers have developed various strategies to optimize the formulation of biological ink. For example, increasing the viscosity of biological ink to reduce the settlement speed of cells, or using specific biological ink additives such as particle gel to improve cell distribution and survival rate. The above solutions solve the problem of cell settlement, but also limit the types of biological ink used. How to ensure the uniformity of biological ink cells before application has become a problem to be solved. SUMMARY

[0004] In order to solve the deficiencies of the prior art, the utility model provides a resuspension cavity pipe based on air pressure change and biological ink resuspension nozzle device, which realizes the re-mixing of biological ink and improves the cell uniformity of biological ink.

[0005] The technical effects achieved by the utility model are realized through the following aspects:

[0006] In a first aspect, the utility model provides a resuspension cavity pipe, comprising:

[0007] A resuspension end, the resuspension end comprises a central column and an outer peripheral wall arranged outside the central column, a mixing cavity for mixing liquid is arranged between the central column and the outer peripheral wall, and the mixing cavity is in communication with the outside at the end of the resuspension end; and

[0008] A fixed end for connecting with an external structure.

[0009] In some implementations, the mixing cavity is an annular structure, and the end of the central column protrudes outward from the opening of the annular structure.

[0010] In some implementations, the surface of the end of the central column protruding outward is an arc surface.

[0011] In some implementations, the spacing between the central column and the peripheral wall is equal.

[0012] In the present implementation, by providing the central column, the liquid entering the re-suspension cavity tube can be effectively guided to fall, and vortexes are avoided from being formed in advance during falling, so that the biological ink is mixed more evenly.

[0013] In a second aspect, the utility model provides a biological ink re-suspension nozzle device, including:

[0014] The re-suspension cavity tube according to any one of the above implementations;

[0015] The needle tube assembly includes a shell and a suspension tube, the suspension tube is arranged in the shell, the re-suspension cavity tube is arranged in the cavity of the suspension tube, and a gap for accommodating liquid is left between the re-suspension cavity tube and the suspension tube; one end of the needle tube assembly is provided with a vent, and the vent is communicated to the cavity of the suspension tube;

[0016] The printing assembly is arranged at the other end of the needle tube assembly opposite to the vent, the printing assembly can be communicated to the cavity of the suspension tube, and is used for controlling the outflow and blockage of the liquid.

[0017] In some implementations, the needle tube assembly further includes a cover, which is sealingly connected to the opening of one end of the suspension tube, the vent is arranged on the side of the cover away from the suspension tube, a plurality of air holes are arranged on the cover, and the vent is communicated to the inside of the suspension tube through the air holes.

[0018] In some implementations, the cover extends from the opening of the suspension tube to the cavity, the cover extends from the opening of the suspension tube to the cavity to form an extension part, a plurality of air holes are arranged on the side surface of the extension part, and a connecting piece is arranged on the end part of the extension part and connected to the fixed end.

[0019] In some implementations, a fixing block is arranged on the cover, and the fixing block fixes the cover on the shell.

[0020] In some implementations, the printing assembly includes a pinch valve, a nozzle, and a fixing support, the pinch valve and the nozzle are arranged on the fixing support, the suspension tube and the nozzle are communicated through a transfusion tube, the pinch valve is connected to the transfusion tube, and is used for controlling the outflow and blockage of the liquid in the transfusion tube.

[0021] In some implementations, the biological ink re-suspension nozzle device further includes a temperature control device, which is connected to the shell and is used for temperature control of the liquid.

[0022] The temperature control device is arranged outside the shell, and comprises a refrigeration part and a heat sink, one side of the refrigeration part is connected with the shell, the other side is connected with the heat sink, and the refrigeration part is a semiconductor refrigeration sheet.

[0023] In summary, the utility model has at least the following advantages:

[0024] The heavy suspension cavity pipe and the biological ink heavy suspension nozzle device based on the air pressure change have the advantages that the heavy suspension cavity pipe is arranged in the suspension pipe, liquid in the suspension pipe is repeatedly oscillated by using the air pressure difference conversion, the diffusion speed of cells in the liquid during the oscillation process is improved by the design of the mixing cavity of the cavity pipe, the mixing effect of the cells in the biological ink in the biological ink heavy suspension nozzle device is improved, and the uniformity of the cells in the biological ink is improved. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 It is a whole structure schematic view of the heavy suspension cavity pipe of the utility model embodiment 1.

[0026] Figure 2 It is a whole structure A-A section view schematic view of the heavy suspension cavity pipe of the utility model embodiment 1.

[0027] Figure 3 It is a simulation effect schematic view of the heavy suspension cavity pipe of the utility model embodiment 1.

[0028] Figure 4 It is a whole structure schematic view of the biological ink heavy suspension nozzle device of the utility model embodiment 2.

[0029] Figure 5 It is a whole structure B-B section view schematic view of the biological ink heavy suspension nozzle device of the utility model embodiment 2.

[0030] Figure 6 It is an explosion schematic view of the needle pipe assembly of the utility model embodiment 2.

[0031] Figure 7 It is a section schematic view when the external air pressure is greater than the air pressure in the mixing cavity of the utility model embodiment 2.

[0032] Figure 8 It is a section schematic view when the external air pressure is less than the air pressure in the mixing cavity of the utility model embodiment 2.

[0033] Figure 9 It is a cover structure schematic view of the utility model embodiment 2.

[0034] Figure 10The fixed block of the embodiment 2 of the utility model is connected with the cover and the shell connection structure schematic view.

[0035] Figure 11 The shell of the embodiment 2 of the utility model is connected with the printing assembly connection structure schematic view.

[0036] Figure 12 The temperature control device explosion structure schematic view of the embodiment 3 of the utility model.

[0037] Figure 13 The biological ink resuspension nozzle device resuspension work flow chart of the embodiment 3 of the utility model.

[0038] Markings in the figure:

[0039] 1, printing assembly;11, nozzle;12, fixed support;13, pinch valve;131, infusion tube;

[0040] 2, needle tube assembly;21, suspension tube;22, shell;221, window;23, cover;231, air hole;232, connecting piece;24, fixed block;25, air vent;

[0041] 3, resuspension cavity tube;31, fixed end;32, resuspension end;321, mixing cavity;322, center column;323, peripheral wall;

[0042] 4, temperature control device;41, refrigeration part;42, radiator. DETAILED DESCRIPTION

[0043] In order to make the purpose, technical scheme and advantage of the utility model embodiment clearer, the following will combine the drawings in the utility model embodiment, and the technical scheme in the utility model embodiment is clearly and completely described. The described embodiment is a part of the embodiment of the utility model, rather than all the embodiments.

[0044] Therefore, the following detailed description of the embodiment of the utility model provided in the drawings is not intended to limit the scope of the claimed utility model, but only represents selected embodiments of the utility model. Based on the embodiments in the utility model, all other embodiments obtained by those of ordinary skill in the art without creative labor are within the scope of protection of the utility model.

[0045] Embodiment 1:

[0046] Please refer to the attached Figures 1-3The utility model discloses a kind of heavy suspension cavity tubes 3 based on air pressure variation, including heavy suspension end 32, heavy suspension end 32 includes center column 322 and the outer peripheral wall 323 of being arranged at the outside of center column 322, center column 322 with the outer peripheral wall 323 between being equipped with for liquid mixing mixing cavity 321, mixing cavity 321 at the end of heavy suspension end 32 with external communication;And fixed end 31, for with external structure connection.

[0047] Specifically, heavy suspension cavity tube 3 is in columnar structure, and two ends are respectively set as heavy suspension end 32 and fixed end 31, fixed end 31 can be selected but not limited to screw groove, fixed end 31 is adaptively connected with other components, and the shape of fixed end 31 is not limited here, heavy suspension end 32 is provided with center column 322, and outer peripheral wall 323 is arranged outside center column 322, and the part formed between outer peripheral wall 323 and center column 322 is mixing cavity 321, and mixing cavity 321 can be used for containing liquid, and only one opening is communicated outward, and the opening direction is set to the side away from fixed end 31.

[0048] When using, heavy suspension end 32 of heavy suspension cavity tube 3 is towards and at least partially immersed in liquid to be mixed, so that the opening of heavy suspension end 32 is completely located below the liquid level of liquid to be mixed, when liquid is flowed into the opening of mixing cavity 321 under the influence of external air pressure, the liquid level in mixing cavity 321 rises, until the internal air pressure of mixing cavity 321 reaches the same time as external air pressure, the liquid level stops rising, when the external air pressure is changed from positive pressure to negative pressure or zero pressure, the liquid in mixing cavity 321 is affected by internal air pressure and gravity and falls back to the opening direction, the falling back liquid impacts external liquid, which is beneficial to the diffusion of cells in liquid, so as to achieve liquid mixing effect.

[0049] Further, please continue to refer to Figure 1 And Figure 2 In the embodiment, mixing cavity 321 is annular structure, and the end of center column 322 protrudes outward from the annular opening.

[0050] Specifically, mixing cavity 321 is annular structure, and the end of center column 322 extends out of the opening of mixing cavity 321, and the end protrudes outward from the annular opening, and the protruding part can guide the falling back of liquid, so as to form vortex at the end of heavy suspension end 32.

[0051] In addition, in order to improve the mixing efficiency of liquid in the process of oscillation in heavy suspension cavity tube 3, the surface of the end of center column 322 protruding outward is arc surface.

[0052] Specifically, the end of center column 322 extends outward and protrudes, and the surface of the end protruding outward is changed into arc surface. Figure 3It can be seen that the liquid falling direction is offset along the camber direction, and the falling liquid converges towards the camber center, thereby forming a vortex to make the liquid mixing more uniform and the mixing efficiency higher.

[0053] In order to improve the falling speed of the liquid during the liquid oscillation in the heavy suspension cavity tube 3, the distance between the center column 322 and the outer peripheral wall 323 is equal.

[0054] Specifically, the inner wall of the mixing cavity 321 is composed of the center column 322 and the outer peripheral wall 323, and the inner wall is arranged in parallel to the direction of the central axis of the heavy suspension cavity tube 3. From the top of the mixing cavity 321 to the bottom of the mixing cavity 321, the distance between the center column 322 and the outer peripheral wall 323 is equal everywhere. When mixing, since the volume of the liquid contained in the mixing cavity 321 is uniform everywhere, the liquid is prevented from colliding with each other in the mixing cavity 321 in advance when falling, and the speed of the liquid reaching the opening at the end of the center column 322 when falling is affected.

[0055] In the embodiment, the heavy suspension cavity tube 3 has a columnar structure, and two ends are respectively provided as a heavy suspension end 32 and a fixed end 31. The center column 322 and the outer peripheral wall 323 outside the center column 322 are arranged at the heavy suspension end 32, and the part between the center column 322 and the outer peripheral wall 323 forms the mixing cavity 321. The liquid surface in the mixing cavity 321 can be raised and fallen repeatedly by using the pressure difference, and the liquids collide with each other, thereby realizing the mixing effect of the biological ink. Compared with the traditional method of optimizing the biological ink formula or the biological ink mixed by the traditional mechanical stirring method, the uniformity of the cells is higher, and the mixing efficiency is also higher.

[0056] The inner wall of the mixing cavity 321 is composed of the center column 322 and the outer peripheral wall 323, and the design of the inner wall parallel to the central axis of the heavy suspension cavity tube 3 is adopted. The liquid in the mixing cavity 321 can be guided by the inner wall when falling, and the liquid is prevented from colliding with each other in the mixing cavity 321 in advance when falling, and the speed of the liquid reaching the opening at the end of the center column 322 when falling is affected. The annular structure realizes the uniform diffusion of the liquid in all directions to achieve a better mixing effect. In addition, the end of the center column 322 is provided with a convex camber, so that the liquid converges towards the center and forms a vortex at the end, thereby improving the overall mixing efficiency.

[0057] It can be understood that the heavy suspension cavity tube based on the change of the gas pressure of the utility model can achieve the goals of uniform fusion of the liquid, acceleration of the reaction process, improvement of the characteristics or behavior of the liquid, and the like when the biological ink is transmitted, so as to meet the special application requirements of the biological ink in the fields of pharmacy, chemical research, material preparation, biotechnology, and chemical production, and the application scope is not limited to biological 3D printing.

[0058] Embodiment 2:

[0059] The embodiment is a bio-ink resuspension nozzle device using the above-mentioned resuspension cavity tube based on air pressure change.

[0060] Please refer to Figures 4-5 The bio-ink resuspension nozzle device includes a resuspension cavity tube 3, a needle tube assembly 2, the needle tube assembly 2 includes a shell 22 and a suspension tube 21, the suspension tube 21 is arranged in the shell 22, the resuspension cavity tube 3 is arranged in the cavity of the suspension tube 21, and a gap for accommodating liquid is left between the resuspension cavity tube 3 and the suspension tube 21; one end of the needle tube assembly 2 is provided with a vent 25, the vent 25 is communicated to the cavity of the suspension tube 21; a printing assembly 1 is arranged at the other end of the needle tube assembly 2 opposite the vent 25, and the printing assembly 1 can be communicated to the cavity of the suspension tube 21 for controlling the outflow and blockage of liquid.

[0061] Specifically, the bio-ink resuspension nozzle device includes a resuspension cavity tube 3, a needle tube assembly 2, a printing assembly 1 and a temperature control device 4, the needle tube assembly 2 includes a shell 22 and a suspension tube 21, the suspension tube 21 is arranged in the shell 22, the needle tube assembly 2 is provided with a vent 25 at the top end for communicating with an external air pressure control mechanism, the resuspension cavity tube 3 is arranged in the suspension tube 21, and a gap for liquid flow is left between the resuspension cavity tube 3 and the suspension tube 21, the printing assembly 1 is connected to the bottom of the needle tube assembly 2, and the printing assembly 1 can be communicated to the cavity of the suspension tube 21 to control the outflow and blockage of liquid in the suspension tube 21.

[0062] Preferably, the shell 22 has a polytetrafluoroethylene coating layer to avoid heat or cold loss to the greatest extent, and the suspension tube 21 is made of transparent material such as polyurethane, organic glass, glass and transparent PE, and a window 221 is arranged on the side of the shell 22 to facilitate observation of the mixing or sedimentation of the sample and the remaining amount of liquid in the suspension tube 21.

[0063] In some embodiments, as shown in Figure 6 The needle tube assembly 2 further includes a cover 23 sealingly connected to the opening of one end of the suspension tube 21, the vent 25 is arranged on the side of the cover 23 away from the suspension tube 21, a plurality of air holes 231 are arranged on the cover 23, and the vent 25 is communicated to the inside of the suspension tube 21 through the air holes 231.

[0064] Specifically, the cover 23 is arranged at the top of the needle tube assembly 2, the vent 25 is arranged at the top of the cover 23, the bottom of the cover 23 is sealingly connected to the opening of the top of the suspension tube 21, the air holes 231 arranged on the bottom of the cover 23 are located on the inside of the opening of the top of the suspension tube 21, and the vent 25 is communicated to the inside of the suspension tube 21 through the air holes 231.

[0065] In use, as shown in Figures 7-8As shown, when the biological ink needs to be mixed when the suspension in the biological ink resuspension nozzle device is settled, the liquid level in the suspension pipe 21 at least partially reaches the opening position of the mixing cavity 321 of the resuspension cavity pipe 3, the external air pressure control mechanism provides gas into the inside of the suspension pipe 21, the gas is inhaled from the air inlet 25 and exhaled from the air hole 231, the liquid level in the suspension pipe 21 is changed due to the influence of air pressure, the air pressure in the suspension pipe 21 is greater than the air pressure in the mixing cavity 321, the liquid level in the suspension pipe 21 drops and enters the mixing cavity 321 of the resuspension cavity pipe 3 from the bottom, the liquid level in the mixing cavity 321 of the resuspension cavity pipe 3 rises, and stops when the air pressure in the mixing cavity 321 is consistent with the air pressure in the resuspension cavity pipe 3, the external air pressure control mechanism exhausts, the air pressure in the suspension pipe 21 changes from positive pressure to negative pressure or zero pressure, the gas in the suspension pipe 21 is discharged through the air hole 231, the internal air pressure decreases, the air pressure in the mixing cavity 321 is greater than the air pressure in the suspension pipe 21, so that the liquid in the mixing cavity 321 falls back, and the liquid level in the suspension pipe 21 rises. The air pressure in the biological ink resuspension nozzle device is changed repeatedly and intermittently, the biological ink rises and falls multiple times, and the water hammer effect is used to make the liquid at the bottom of the suspension pipe 21 impact continuously and make the cells rapidly diffuse in the liquid, thereby achieving the purpose of pneumatically mixing the biological ink.

[0066] In some embodiments, as shown in Figure 9 The cover 23 extends from the opening of the suspension pipe 21 into the cavity, a plurality of air holes 231 are arranged on the side of the extension, and a connecting piece 232 is arranged at the end of the extension. The connecting piece 232 is connected with the fixed end 31.

[0067] Specifically, the cover 23 is sealingly connected with the opening at the top of the suspension pipe 21, the part of the cover 23 inside the opening extends into the cavity as an extension, a plurality of air holes 231 are arranged on the side of the extension, and a connecting piece 232 is arranged at the end of the extension. The connecting piece 232 is detachably connected with the fixed end 31 of the resuspension cavity pipe 3, or the cover 23 is integrally formed with the resuspension cavity pipe 3.

[0068] Specifically, the cover 23 is sealingly connected with the opening at the top of the suspension pipe 21, the part of the cover 23 inside the opening extends into the cavity as an extension, a plurality of air holes 231 are arranged on the side of the extension, and a connecting piece 232 is arranged at the end of the extension. The connecting piece 232 is detachably connected with the fixed end 31 of the resuspension cavity pipe 3, or the cover 23 is integrally formed with the resuspension cavity pipe 3.

[0069] In some embodiments, as shown in Figure 10As shown, the cover 23 can be provided with a fixing block 24, which fixes the cover 23 on the shell 22.

[0070] Specifically, the fixing block 24 is arranged on the cover 23, and the fixing block 24 connects the cover 23 and the shell 22, avoids opening a hole on the suspension pipe 21, and connects the suspension pipe 21 to the shell 22 through the fixing block 24. The fixing block 24 is reinforced, the sealing effect of the cover 23 and the opening at the top of the suspension pipe 21 is increased, the opening sealing part can withstand greater air pressure, and the sealing stability in the suspension pipe 21 can be realized.

[0071] In some embodiments, as Figure 11 As shown, the printing assembly 1 includes a pinch valve 13, a nozzle 11, and a fixing support 12, the pinch valve 13 and the nozzle 11 are arranged on the fixing support 12, a liquid delivery pipe 131 is arranged on the pinch valve 13, and the liquid delivery pipe 131 is communicated to the suspension pipe 21 and the nozzle 11 at two ends, respectively, and the pinch valve 13 controls the outflow and blockage of the liquid in the liquid delivery pipe 131.

[0072] Specifically, the printing assembly 1 includes a pinch valve 13, a nozzle 11, and a fixing support 12, the fixing support 12 is connected to the needle pipe assembly 2, the pinch valve 13 and the nozzle 11 are both installed on the fixing support 12, a liquid delivery pipe 131 is arranged on the pinch valve 13, the liquid delivery pipe 131 is provided with a joint at two ends, the liquid delivery pipe 131 is communicated to the nozzle 11 and the suspension pipe 21 in the needle pipe assembly 2 at two ends, respectively, and the pinch valve 13 can control the liquid flow and blockage in the liquid delivery pipe 131 by electricity or gas. When the suspension liquid is deposited, the pinch valve 13 blocks the liquid flow, avoids the liquid from entering the nozzle 11 due to the air pressure difference when the bio-ink needs to be resuspended, and opens the channel in the liquid delivery pipe 131 when printing is needed. The external air pressure control mechanism controls the air pressure in the suspension pipe 21, realizes the air pressure difference to push the hydrogel containing cells, the inner wall of the nozzle 11 is a microfluidic channel, and precise deposition printing can be realized.

[0073] The utility model discloses a biological ink resuspension nozzle device, the vent 25 that communicates with external air pressure control mechanism is arranged at the top of needle tube subassembly 2, the bottom of needle tube subassembly 2 communicates with printing assembly 1, printing assembly 1 controls the liquid circulation and block, the top of suspension tube 21 is sealedly connected with the cover 23, the bottom of suspension tube 21 communicates with printing assembly 1, and the resuspension cavity pipe 3 is arranged in the extension of cover 23 and is in suspension tube 21. When carrying out the pneumatic mixing of biological ink, the external air pressure control mechanism provides gas into the inside of suspension tube 21, under the driving of the air pressure difference, the liquid level in mixing cavity 321 and suspension tube 21 is changed under the influence of air pressure, and the biological ink at the bottom of suspension tube 21 enters mixing cavity 321, so that the liquid level difference is formed. The external air pressure control mechanism communicates the gas extraction in suspension tube 21, and the air pressure in suspension tube 21 is converted from positive pressure to negative pressure or zero pressure, and the internal air pressure loses balance. The change makes the liquid in mixing cavity 321 convert the pressure and gravity into the kinetic energy of downward impact, utilizes the water hammer effect, the liquid at different positions is exchanged, vortex is generated at the bottom of suspension tube 21, liquid can be brought from the central area at the bottom to the edge area and then brought back to the central area from the edge area, the mixing effect of liquid is further enhanced, the mixing purpose of liquid is achieved, and the problem that biological ink is suspended and settled in the printing process is solved. The structure of the pneumatic mixing biological ink is simple, the mixing effect is obvious through the mutual impact of internal liquid, additional stirring structure or mechanical structure is not needed, and there is no risk of external pollution. The temperature control device 4 can realize the temperature change of the shell 22, thereby affecting the internal environment, achieving the temperature control of the liquid in the suspension tube 21, improving the cell survival rate, and avoiding the pollution risk when the temperature control device 4 works and contacts the shell 22 without contacting the biological ink.

[0074] Embodiment 3:

[0075] This embodiment is further optimized on the basis of the above-mentioned embodiment, please refer to Figure 12 . For the characteristic change of biological ink caused by temperature influence in the printing process, such as MateriGel will form irreversible solidification when higher than 4 degrees Celsius, and GelMA will appear high viscosity degeneration when lower than 37 degrees Celsius, the high and low temperature constant temperature control of biological ink can be realized through specific temperature control device.

[0076] In some embodiments, the biological ink resuspension nozzle device further comprises a temperature control device 4 connected with the shell 22 for temperature control of the liquid, the temperature control device 4 is arranged outside the shell 22, and the temperature control device 4 comprises a refrigeration part 41 and a radiator 42, one side of the refrigeration part 41 is connected with the shell 22, and the other side is connected with the radiator 42.

[0077] Specifically, the temperature control device 4 is mounted on the outside of the housing 22 and electrically connected to the temperature control device 4. Heat can be directly transferred between the housing 22 to achieve changes in the ambient temperature inside the housing 22. The suspension tube 21 located within the housing 22 is affected, thereby achieving the effect of controlling the temperature of the bio-ink in the suspension tube 21. The radiator 42 is provided with a fin structure, which is used to increase the heat dissipation area. The contact area between the radiator 42 and the cooling element 41 is made of a metal material with excellent thermal conductivity. The heat exchange method of the radiator 42 is liquid heat exchange or gas heat exchange. The contact surface between the cooling element 41 and the housing 22 is filled with thermal conductive silicone grease. The contact surface between the cooling element 41 and the radiator 42 is also filled with thermal conductive silicone grease to increase the thermal conductivity efficiency. The semiconductor refrigeration chip can achieve cold end and hot end exchange by changing the direction of the current, providing cooling or heating for the housing 22, so that the bio-ink in the suspension tube 21 is maintained at a low or high temperature.

[0078] For the appropriate temperature required for cell culture, the biological ink can be accurately maintained at 37°C through the temperature control device 4. The specific selection and temperature control strategy of the temperature control device 4 need to be determined comprehensively based on multiple factors such as the type of biological ink, printing process requirements, and cell characteristics, and must be experimentally tested and optimized to ensure the accuracy and stability of temperature control.

[0079] In this embodiment, if Figure 13 As shown, the bio-ink resuspension nozzle device can be applied in the field of bio-ink 3D printing. When the bio-ink resuspension nozzle device is in operation, the external air pressure control mechanism inputs a positive pressure air source into the suspension tube 21 to drive the bio-ink resuspension nozzle device to print normally. During the printing process, the temperature control device 4 continues to operate to maintain a temperature suitable for the preservation of the bio-ink. At this time, the pinch valve 13 is closed, allowing the bio-ink to be subjected to air pressure and flooded into the mixing chamber 321. The positive pressure in the bio-ink is then switched to a negative pressure or zero pressure state. The bio-ink is subjected to a water hammer effect and violently impacts the bottom of the suspension tube 21, achieving bio-ink mixing. The bio-ink in the suspension tube 21 is observed to see if the cell particles in the bio-ink are resuspended. If the desired effect is not achieved, the process returns to the step of closing the pinch valve 13, re-pressurizing the bio-ink, and repeating the mixing step. If the cell particles in the bio-ink have been resuspended, the pinch valve 13 is opened and the printing process is continued.

[0080] The bio-ink resuspension nozzle device of the present invention improves the range of biomaterial selection and cell survival rate by adopting a temperature control device 4. At the same time, the radiator 42 can achieve temperature control more efficiently. The semiconductor refrigeration plate ensures the cooling effect while changing the current direction to achieve the heating effect.

[0081] In the utility model, unless another definite provision and limitation, the terms "mount", "link", "connect", "fix" and so on should do the broad sense understanding, for example, can be fixed connection, also can be detachable connection, or be integrated;Can be mechanical connection, also can be electrical connection;Can be direct connection, also can indirectly connect through the intermediate medium, can be two element internal communication or two element mutual action relation.For the ordinary skilled person in the art, the specific meaning of the above terms in the utility model can be understood according to the specific circumstances.

[0082] In the description of the utility model, it needs to be explained that the orientation or position relation indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "internal", "external" and so on is based on the orientation or position relation shown in the drawing, or the orientation or position relation of the utility model product when it is usually placed, which is only for the convenience of describing the utility model and simplifying the description, and does not indicate or imply that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, so it cannot be understood as a limitation on the utility model.In addition, the terms "first", "second", "third" and so on are only used for differentiation in description, and cannot be understood as indicating or implying relative importance.

[0083] In addition, the terms "horizontal", "vertical", "overhang" and so on do not mean that the component must be absolutely horizontal or overhanging, but can be slightly inclined."Horizontal" only means that its direction is more horizontal relative to "vertical", and does not mean that the structure must be completely horizontal, but can be slightly inclined.

[0084] In the utility model, unless another definite provision and limitation, the first feature above or below the second feature can include that the first and second features are in direct contact, or that the first and second features are not in direct contact but are in contact through another feature between them.Moreover, the first feature above, above and above the second feature includes that the first feature is directly above and obliquely above the second feature, or only indicates that the horizontal height of the first feature is higher than that of the second feature.The first feature below, below and below the second feature includes that the first feature is directly below and obliquely below the second feature, or only indicates that the horizontal height of the first feature is less than that of the second feature.

[0085] Although the description of the utility model is combined with the above specific embodiments, it is obvious that many substitutions, modifications and changes can be made by the person skilled in the art according to the above content.Therefore, all such alternatives, improvements and changes are included in the spirit and scope of the appended claims.

Claims

1. A resuspension cavity tube based on air pressure change, characterized in that: include: a resuspension end, the resuspension end comprising a central column and an outer peripheral wall disposed outside the central column, a mixing chamber for liquid mixing being disposed between the central column and the outer peripheral wall, the mixing chamber being in communication with the outside at an end of the resuspension end; and Fixed end, used for connection to external structure.

2. The resuspension cavity tube based on air pressure change according to claim 1, characterized in that: The mixing chamber is an annular structure, and the end of the central column protrudes outward from the annular opening.

3. The resuspension cavity tube based on air pressure change according to claim 2, characterized in that: The outwardly protruding surface of the end portion of the central column is a curved surface.

4. A resuspension cavity tube based on air pressure change according to any one of claims 1 to 3, characterized in that: The central column and the outer peripheral wall have equal spacings.

5. A bio-ink resuspension nozzle device, characterized in that: include: The resuspension cavity tube according to any one of claims 1 to 4; A needle tube assembly comprising a housing and a suspension tube, wherein the suspension tube is disposed within the housing, the resuspension cavity tube is disposed within the cavity of the suspension tube, and a gap for accommodating liquid is left between the resuspension cavity tube and the suspension tube; a vent is provided at one end of the needle tube assembly, the vent being connected to the cavity of the suspension tube; and A printing assembly is arranged at the other end of the needle assembly opposite to the vent, and the printing assembly can be connected to the cavity of the suspension tube to control the outflow and blocking of the liquid.

6. The bio-ink resuspension nozzle device according to claim 5, characterized in that: The needle assembly further comprises: The sealing cover is sealed and connected to an opening at one end of the suspension tube. The vent is arranged on a side of the sealing cover away from the suspension tube. The sealing cover is provided with a plurality of air holes, and the vent is communicated with the interior of the suspension tube through the air holes.

7. The bio-ink resuspension nozzle device according to claim 6, characterized in that: The cover extends an extension portion from the opening of the suspension tube into the cavity, a plurality of air holes are provided on the side of the extension portion, and a connecting piece is provided at the end of the extension portion, and the connecting piece is connected to the fixed end.

8. The bio-ink resuspension nozzle device according to claim 6, characterized in that: A fixing block is provided on the cover, and the fixing block fixes the cover on the shell.

9. The bio-ink resuspension nozzle device according to claim 5, characterized in that: The printing assembly includes a pinch valve, a nozzle, and a fixed bracket. The pinch valve and the nozzle are arranged on the fixed bracket. The suspension tube and the nozzle are connected through an infusion tube. The pinch valve is connected to the infusion tube to control the outflow and blocking of the liquid in the infusion tube.

10. The bio-ink resuspension nozzle device according to claim 5, characterized in that: The device further comprises a temperature control device, which is connected to the housing and is used to control the temperature of the liquid; The temperature control device is arranged outside the shell, and includes a refrigeration element and a radiator. One side of the refrigeration element is connected to the shell, and the other side is connected to the radiator. The refrigeration element is a semiconductor refrigeration plate.

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