Cassette library preparation system

Abstract

The utility model discloses a card box type library preparation system, which belongs to the technical field of gene sequencing instruments and is characterized in that a multi-gun module is arranged in a narrow shell, and a reaction module and a control module are respectively arranged on two sides of the multi-gun module. The gun arranging module comprises a gantry bracket and a pipette, the gantry bracket is provided with a first driving mechanism for driving the pipette to vertically move, and the lower end of the gantry bracket is provided with a first notch for the reaction module to pass through; the control module is installed on the fixed frame, and the frame is provided with a second notch for the reaction module to penetrate through. And a second driving mechanism for driving the reaction module to horizontally and linearly move is also arranged in the narrow shell. A clamping box with an anti-pollution structure is arranged on the reaction module, a reagent tube is arranged in the clamping box, and the reagent tube can be moved to the position under the pipette under the action of a second driving mechanism. In addition, a modular temperature control assembly for the reagent tube is arranged in the reaction module. The system is compact in structure, space is saved, temperature zone crosstalk is avoided through modular temperature control, and the problem of open type pipetting pollution is solved through the anti-pollution clamping box.

Description

Technical Field

[0001] The utility model belongs to the technical field of gene sequencing instruments, and relates to an automated cartridge-type library preparation system with integrated closed cartridges, independent control of multiple temperature zones, and pollution prevention. Background Art

[0002] Gene sequencing is of great significance in fields such as life science research and clinical diagnosis. As a key prerequisite for gene sequencing, library construction technology directly impacts the quality and reliability of sequencing results. However, current gene sequencing library construction technology faces many challenges that need to be addressed.

[0003] In terms of contamination control, existing technologies use an open pipetting design, which greatly increases the risk of contamination. The aerosol contamination rate exceeds 1%, and the sample cross-residue rate is as high as 3.7%, seriously affecting the purity of the sample and the accuracy of sequencing. In terms of equipment performance, traditional library construction equipment is complex, inefficient, and bulky. It requires a wide-body layout, takes up a lot of space, and is difficult to maintain, increasing the cost of use and the difficulty of operation. The temperature control link is also not optimistic. The temperature zone crosstalk phenomenon causes the temperature difference of the PCR reaction to reach ±1.8°C, which greatly affects the stability of the reaction and interferes with the accuracy of the sequencing results. Utility Model Content

[0004] The purpose of the utility model is to provide a cartridge-type library preparation system, which improves integration and reduces floor space through the rational layout of internal components, and at the same time improves the reaction module to solve problems such as temperature zone crosstalk.

[0005] The purpose of this utility model is achieved through the following technical solutions:

[0006] A cartridge-type library preparation system includes a narrow housing, a gun-loading module disposed within the narrow housing, a reaction module and a control module disposed on either side of the gun-loading module, the gun-loading module comprising a gantry support and a plurality of pipettes, each pipette corresponding to a group of reaction process samples; a first drive mechanism for driving the pipettes to move vertically is disposed on the gantry support, and a first notch is formed at the lower end of the gantry support for the reaction module to pass through;

[0007] The control module is arranged on a fixed frame, and the fixed frame has a second gap for the reaction module to pass through;

[0008] A second driving mechanism for driving the reaction module to move horizontally and linearly is provided in the narrow housing; a cartridge with an anti-pollution structure is provided on the reaction module, and a reagent tube is placed in the cartridge. Driven by the second driving mechanism, the reaction module moves horizontally so that the reagent tube in the cartridge is directly below the pipette gun;

[0009] The reaction module is internally provided with a modular temperature control assembly for the reagent tube.

[0010] The overall size of the narrow housing is 530*220*450 mm (deep*width*height), and the space utilization is increased by 40%.

[0011] As a further improvement of an embodiment of the utility model, a plurality of sample grooves are arranged in the card box and are physically isolated from each other, and the reagent tubes are independently placed in the sample grooves; a cover plate is arranged above the sample grooves, and the cover plate is provided with an opening corresponding to each reagent tube, so that the reagent tube can be operated by the pipette through the opening.

[0012] As a further improvement of an embodiment of the utility model, one end of the cover plate is rotatably arranged on the box body of the card box through a pivot, at least two first positioning blocks are arranged on the box body and are rotatably arranged on the box body through the pivot; when the cover plate is closed on the sample groove, rotating the first positioning block can make one end of the first positioning block above the other end of the cover plate, so as to fix the position of the cover plate.

[0013] The above structure can press the reagent tube to make it in excellent contact with the lower sleeve (heating / cooling).

[0014] As a further improvement of an embodiment of the utility model, a clamping plate positioning groove is arranged between the two adjacent sample grooves, and a first clamping groove corresponding to the clamping plate positioning groove is arranged on the cover plate; further comprising a clamping plate, the clamping plate passes through the first clamping groove and is inserted into the clamping plate positioning groove, so as to fix the position of the clamping plate.

[0015] As a further improvement of an embodiment of the utility model, the inner wall of the sample groove is sprayed with a hydrophobic coating, the hydrophobic coating is a parylene coating, and the contact angle is > 120°. The above hydrophobic coating contains a microstructure.

[0016] As a further improvement of an embodiment of the utility model, the reagent tube is a standard eight-way tube. An ultraviolet lamp is arranged on the gantry support, the ultraviolet lamp faces the pipette, and ultraviolet disinfection is performed on the inside of the instrument (not limited to the pipette).

[0017] As a further improvement of an embodiment of the utility model, a reagent box is arranged on the reaction module, the reagent box has a gun head replacement area and a cleaning area, and is used for replacing and cleaning the gun head of the pipette.

[0018] As a further improvement of an embodiment of the utility model, a fluorescence scanning device for the reagent tube is arranged in the reaction module.

[0019] As a further improvement of the embodiment of the utility model, wherein, the modular temperature control assembly includes red copper heating plate and temperature zone heat insulation plate, the red copper heating plate is provided with two groups of sleeve pipes controlled by different temperature control systems, the sleeve pipe is for reagent tube insertion, the temperature zone heat insulation plate is provided with heat insulation hole for sleeve pipe insertion.

[0020] As a further improvement of the embodiment of the utility model, wherein, the liquid path module connected with the pipette in the row gun module is an independent closed liquid path system, which is isolated from the electrical circuit space.

[0021] As a further improvement of the embodiment of the utility model, wherein, the first driving mechanism includes a bipolar transmission structure, including a first screw nut transmission mechanism and a second screw nut transmission mechanism, the first screw nut transmission mechanism is arranged on the gantry support and the nut thereof is connected with the first bearing plate, the second screw nut transmission mechanism is arranged on the first bearing plate and the nut thereof is connected with the second bearing plate, the second bearing plate is fixedly provided with a support rod, and the support rod is fixedly connected with the pipette.

[0022] As a further improvement of the embodiment of the utility model, wherein, the reaction module located outside the cartridge is provided with a gun head replacement area and a cleaning area for replacing and cleaning the gun head of the pipette.

[0023] The above technical scheme has the following beneficial effects: compact structure layout, reducing the occupied space, adopting modular temperature control design, avoiding temperature zone crosstalk phenomenon by relying on temperature zone heat insulation plate; adopting the anti-pollution structure cartridge solves the pollution problem caused by open pipetting design; the liquid path module is isolated from the electrical circuit space, eliminating the risk of short circuit; the row gun module and the reaction module are combined and applied, realizing the trinity of piercing, liquid injection and cleaning. BRIEF DESCRIPTION OF DRAWINGS

[0024] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the following will briefly introduce the drawings needed to be used in the embodiment or prior art description. Obviously, the drawings in the following description are only exemplary, and those skilled in the art can obtain other implementation drawings according to the provided drawings without creating creative labor.

[0025] The structure, proportion, size, etc. shown in the specification are only used to cooperate with the content disclosed in the specification, so that those skilled in the art can understand and read, and are not used to limit the implementation conditions of the utility model, so they do not have technical significance. Any modification of structure, change of proportion relationship or adjustment of size, without affecting the effect and purpose of the utility model, should still fall within the scope of the technical content disclosed by the utility model.

[0026] Figure 1 The explosion structure schematic view is provided for the utility model.

[0027] Figure 2 The combined structure schematic view is provided for the utility model.

[0028] Figure 3 The card box and reagent tube combined three-dimensional schematic view is provided for the utility model.

[0029] Figure 4 The card box and reagent tube combined structure schematic view is provided for the utility model.

[0030] Figure 5 The modular temperature control assembly schematic view is provided for the utility model.

[0031] Figure 6 The modular temperature control assembly side view combined schematic view is provided for the utility model.

[0032] Figure 7 The modular temperature control assembly overhead schematic view is provided for the utility model.

[0033] Figure 8 The structure schematic view of a group of reaction flow samples is provided for the utility model.

[0034] Figure 9 The three-dimensional schematic view of the reagent box is provided for the utility model.

[0035] Figure 10 The structure schematic view of the reagent box is provided for the utility model.

[0036] In the drawing:

[0037] 1, narrow shell;

[0038] 11, control panel;

[0039] 12, discharge window;

[0040] 2, row gun module;

[0041] 21, gantry support;

[0042] 22, pipette;

[0043] 23, first gap;

[0044] 3, reaction module;

[0045] 31, card box;

[0046] 311, sample groove; 312, cover plate; 313, first positioning block; 314, card plate positioning groove; 315, first card slot;

[0047] 32, replace the gun head area;

[0048] 33, cleaning area;

[0049] 34, red copper heating plate;

[0050] 35, temperature zone heat insulation plate;

[0051] 4, control module;

[0052] 5, fixed frame;

[0053] 51, second notch;

[0054] 6, second drive mechanism;

[0055] 7, reagent tube;

[0056] 8, reagent kit. DETAILED DESCRIPTION

[0057] It should be noted that the embodiments in the present application and the features in the embodiments can be combined with each other without conflict. The present application will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0058] It should be noted that, unless otherwise specified, all technical and scientific terms used in the present application have the same meaning as generally understood by those skilled in the art to which the present application belongs.

[0059] In the present application, unless otherwise stated, the orientation words such as "up, down, top, bottom" are generally directed to the direction shown in the drawings, or are directed to the vertical, perpendicular or gravity direction of the components themselves; similarly, for the convenience of understanding and description, "inner, outer" refers to the inner and outer of the contour of each component itself, but the above orientation words are not used to limit the present application.

[0060] Referring to Figures 1-7 As shown in the figure, a cassette library preparation system includes a narrow shell 1 as an external support and protection structure of the entire system, which provides a stable installation environment for the internal modules. The overall size of the narrow shell 1 in the present embodiment is 530x220x450 mm (deepxwidexhigh), which effectively reduces the space occupied by the entire system by adopting a compact design, and the space utilization rate is improved by 40%, which is convenient for arrangement and operation in limited experimental site.

[0061] The narrow shell 1 is provided with a row gun module 2, and the row gun module 2 is provided with a reaction module 3 and a control module 4 on both sides. The row gun module 2 includes a gantry support 21 and a plurality of pipettes 22, and the gantry support 21 is provided with a first driving mechanism for driving the pipettes 22 to move vertically, so as to realize the precise movement of the pipettes in the vertical direction and meet the requirements of different experimental operations on the pipette height. In the embodiment, the pipettes 22 are four, and each pipette 22 corresponds to a group of reaction process samples. That is, the card box type library preparation system can simultaneously test four groups of samples.

[0062] The gantry support 21 is designed with a first gap 23 at the lower end. The first gap 23 provides the necessary space for the flexible movement of the reaction module 3, so that the reaction module 3 can smoothly complete the horizontal linear motion in the system.

[0063] The control module 4 is arranged on the fixed frame 5, and the fixed frame 5 is provided with a second gap 51 for the reaction module 3 to pass through, so as to provide the necessary space for the flexible movement of the reaction module 3. In the embodiment, the electrical module is also arranged on the fixed frame 5, so that it is in the same area as the control module 4, which is convenient for modular management. The control module 4 serves as the "brain" of the whole system, and is responsible for coordinating and controlling the collaborative work between the modules to ensure that the system operates stably according to the preset program. Of course, the card box type library preparation system also has a power module for supplying power to the card box type library preparation system, so that it can operate normally.

[0064] The narrow shell 1 is provided with a second driving mechanism 6 for driving the reaction module 3 to move horizontally. The reaction module 3 is provided with a card box 31 with an anti-pollution structure, and the card box 31 is placed with a reagent tube 7. Under the driving of the second driving mechanism 6, the reaction module 3 moves horizontally, so that the reagent tube 7 in the card box 31 is directly below the pipette 22, thereby realizing efficient pipetting operation. At the same time, the modular temperature control assembly arranged in the reaction module 3 for the reagent tube 7 can independently control the temperature of different reagent tubes 7, effectively avoiding the crosstalk phenomenon between different temperature zones, and ensuring the accuracy and reliability of the experimental results.

[0065] In the card box type library preparation system, the first driving mechanism adopts a bipolar transmission structure to realize the precise movement of the pipettes 22 in the vertical direction. Specifically, the first driving mechanism includes a first screw nut transmission mechanism 24 and a second screw nut transmission mechanism 25. The first screw nut transmission mechanism 24 is installed on the gantry support 21, and the nut is connected with the first bearing plate 26. When the mechanism operates, the nut drives the first bearing plate 26 to move vertically.

[0066] The second screw-nut transmission mechanism 25 is mounted on a first carrier plate 26, with its nut connected to a second carrier plate 27. Movement of the first carrier plate 26 drives the second screw-nut transmission mechanism 25, which in turn enables further vertical movement of the second carrier plate 27. A support rod 28 is secured to the second carrier plate 27, which is in turn fixedly connected to the pipette 22, transmitting the vertical motion to the pipette 22.

[0067] The first screw-nut transmission mechanism 24 and the second screw-nut transmission mechanism 25 have similar structures. Both are screw modules driven by servo motors. Guide components are provided on both sides. The guide columns and guide sleeves cooperate to ensure orderly operation in the vertical direction. This is a conventional driving technology.

[0068] Similarly, the second drive mechanism 6 adopts a third screw-nut transmission mechanism, which is also a structure of a screw module driven by a servo motor and guide components on both sides, which can realize the horizontal linear motion of the reaction module 3 and ensure the coordinated and stable operation of various modules in the system.

[0069] In this embodiment, the combined application of the discharge gun module 2 and the reaction module 3 can realize the three-in-one function of puncture-injection-cleaning, greatly improving the efficiency and automation of experimental operations, reducing the errors and contamination risks that may be caused by manual intervention, and providing a more accurate, efficient and reliable solution for library construction experiments.

[0070] In this embodiment, the narrow housing 1 features a control panel 11 and a reagent dispensing window 12. Dispensing window 12 is located directly above the reaction module 3 and hinged at one end to the housing, allowing it to be flipped open. For easy observation, a visual panel is provided above dispensing window 12, allowing the operator to monitor reagent placement. The control panel 11 is connected to the control module 4, allowing on-site personnel to debug and control the cartridge-based library preparation system.

[0071] Combine Figure 3 、 Figure 4 As shown, the cartridge 31, a key component in the reaction module that holds the reagent tubes 7, is meticulously designed with several physically isolated sample wells 311. These wells 311 are regularly arranged and completely isolated from one another, effectively preventing cross-contamination between samples and providing independent, secure storage space for the reagent tubes 7, which are each placed independently in each sample well 311.

[0072] The sample groove 311 is provided with a cover plate 312 above it, and the cover plate 312 is provided with an opening corresponding to each reagent tube 7. This design allows the liquid handling gun 22 to smoothly pass through the opening to perform puncture, liquid injection, and cleaning operations on the reagent tube 7 without affecting other reagent tubes 7. One end of the cover plate 312 is rotatably installed on the box body of the cartridge 31 through a pivot, facilitating opening and closing. At least two first positioning blocks 313 are also provided on the box body and rotatably installed on the box body through a pivot. When the cover plate 312 is closed on the sample groove 311, the first positioning block 313 only needs to be rotated so that one end is above the other end of the cover plate 312 to stably fix the position of the cover plate 312.

[0073] The cover plate 312 can press the reagent tube 7 in the sample groove 311, so that the reagent tube 7 is in good contact with the sleeve (heating / cooling) below.

[0074] A clamping plate positioning groove 314 is provided between two adjacent sample grooves 311, and a first clamping groove 315 is provided on the cover plate 312. In addition, a clamping plate is provided, which is inserted into the clamping plate positioning groove 314 through the first clamping groove 315, thereby fixing the position of the clamping plate. The above clamping plate divides the reagent tubes 7 into different test units (reaction process samples), facilitating observation and operation by on-site personnel.

[0075] In this embodiment, the inner wall of the sample groove 311 is sprayed with a parylene coating (in addition to the parylene coating, other hydrophobic coatings can also be used, which can include microstructures). The parylene coating makes the contact angle of the inner wall of the sample groove 311 > 120°, has good hydrophobicity, can effectively reduce sample residue, and is convenient for cleaning and disinfection. The reagent tube 7 is a standard eight-way tube, which is convenient for adapting to various experimental equipment. At the same time, the gantry support 21 is also provided with an ultraviolet lamp, which can periodically perform ultraviolet disinfection on the inside of the instrument, effectively killing the last sample and preventing pollution between samples; the ultraviolet lamp uses 365nm UV-C, and other wavelengths of ultraviolet lamps can also be used, such as 222nm far ultraviolet.

[0076] Reference Figures 5-7 As shown, the modular temperature control assembly includes a red copper heating plate 34 and a temperature zone heat insulation plate 35. The red copper heating plate 34 has good heat conduction performance, and two groups of sleeve pipes controlled by different temperature control systems provided on the red copper heating plate 34 can provide accurate and different temperature environments for the reagent tubes 7 inserted therein according to experimental requirements. The temperature zone heat insulation plate 35 can effectively block heat transfer, and the heat insulation holes provided on the temperature zone heat insulation plate 35 are tightly matched with the sleeve pipes, further ensuring that each temperature zone is independent of each other, avoiding temperature zone crosstalk, and ensuring the accuracy of temperature control, so that the PCR temperature control precision can reach ±0.3℃.

[0077] One temperature zone A (-20-15℃) is used for PCR, and the other temperature zone B (0-90℃) is used for reagent storage, and a closed-loop calibration algorithm is adopted: 4 points of temperature are collected per second, and the dynamic compensation of adjacent temperature zone thermal interference (optimization formula) is adopted:

[0078] ΔT_comp= k·e^{-d / s}, (k=0.8, s=5 mm)

[0079] Where d is the temperature zone spacing, and s is the attenuation coefficient.

[0080] As Figure 8 shown is a set of reaction process samples, which consists of reagent strip one and reagent strip two, wherein the reagent strip one adopts a standard eight connected tube, and the numbers from left to right are 1A-1H, and the environment used is a -20℃ reagent loading area; the reagent strip two adopts a standard eight connected tube, and the numbers from left to right are 2A-2H, and the environment used is a 4℃ reagent loading area. The specific installation of reagent functions and use process are as follows:

[0081]

[0082] The reaction module 3 on which the cartridge 31 is located is provided with a replacement gun head area 32 and a cleaning area 33. The replacement gun head area 32 is equipped with a gun head storage device, and the operator can orderly place a plurality of specifications of spare gun heads, so as to facilitate the pipette 22 to quickly replace the appropriate gun head according to the experimental demand, and ensure the accuracy of the pipetting operation. The cleaning area 33 is provided with a cleaning liquid storage container and a cleaning nozzle, and when the pipette 22 completes a pipetting operation, it can be moved to the cleaning area 33, and the cleaning nozzle will spray cleaning liquid to clean the gun head comprehensively, remove the residual reagent, avoid cross contamination, and ensure the accuracy of the subsequent experiment.

[0083] In this embodiment, the replacement gun head area 32 and the cleaning area 33 are integrated into the reagent cartridge 8. As shown in Figure 9 , Figure 10 , the reagent cartridge 8 has eight independent test tubes, and the depths of the test tubes are different and can be customized according to actual conditions. The eight test tubes are sequentially labeled as 3A-3H. In this embodiment, the purposes of each test tube are as follows:

[0084]

[0085] Each reagent cartridge 8 corresponds to a set of reaction process samples, and the two cooperate with each other to realize gene sequencing detection.

[0086] In addition, the fluorescence scanning device for the reagent tube 7 is arranged in the reaction module 3. The device adopts advanced optical scanning technology, can quickly and accurately scan the fluorescence of the reagent tube 7 placed in the sample groove 311, and can obtain the fluorescence signal data of the sample in real time, so as to provide a key basis for experimental analysis, and further improve the functionality of the entire cartridge library preparation system and the reliability of the experimental results.

[0087] The liquid path module connected with the liquid gun 22 in the liquid gun module 2 is designed as a closed liquid path system. This design isolates the liquid path system from the external environment, prevents external impurities from polluting the liquid, and ensures the purity of the experiment. At the same time, the liquid path system is spatially isolated from the electrical circuit, avoiding the influence of electromagnetic interference generated by the electrical circuit on the liquid path system, and preventing the possible leakage of the liquid path system from damaging the electrical circuit, thereby improving the stability and reliability of the operation of the entire liquid gun module 2.

[0088] In use, the reagents required for detection are sequentially placed in the cartridge 31, and the cartridge 31 is placed and fixed on the reaction module as a whole. Close the feeding window 12 and start the device. At this time, the liquid gun 22 first replaces the gun head in the gun head area 32, then returns to the initial position, and waits for the reaction module 3 to come. The reaction module 3 is sequentially moved to the lower side of the liquid gun 22 under the action of the second driving mechanism 6. The liquid gun 22 sequentially moves downward according to the set program, so that the gun head sharp on it pierces the sealing film of the cartridge and sucks the original liquid, and then transfers and injects the original liquid into the reagent tube where the reaction reagent is located. Then go to the cleaning area 33 to clean the gun head, realize the trinity of piercing-liquid injection-cleaning; and wait for the next reagent operation.

[0089] Compared with the existing cartridge library preparation system, the cartridge library preparation system provided by the utility model has obvious advantages in aerosol pollution rate, PCR temperature stability and cross residual rate, and the comparison of test results is as follows:

[0090]

[0091] The utility model adopts compact layout in overall structure design, reasonably plans the installation position of each module, effectively reduces the space occupied by the system, can be arranged flexibly in limited experimental site, and improves the space utilization.

[0092] The modular temperature control design is a highlight. By independently controlling each temperature zone, the temperature is accurately adjusted, mutual interference between different temperature zones is avoided, crosstalk phenomenon of temperature zones is eliminated, and the experiment is ensured to be carried out in a stable temperature environment, and the accuracy of the experimental results is improved.

[0093] The cartridge 31 of the anti-pollution structure provides a closed and independent storage space for the reagent tube, effectively solves the pollution problem that may be caused by the open pipetting design, and guarantees the purity of the sample. The liquid path module is spatially isolated from the electrical circuit, cuts off the possibility of contact between the two from the physical layer, eliminates the risk of short circuit, and enhances the safety and stability of the system. The combination of the gun module 2 and the reaction module 3 realizes the integrated operation of piercing, liquid injection and cleaning, and improves the experimental efficiency.

[0094] Obviously, the above-described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor should belong to the protection scope of the present application.

[0095] It should be noted that the terms used herein are only intended to describe specific embodiments, and are not intended to limit the exemplary embodiments according to the present application. As used herein, the singular form is intended to include the plural form unless the context clearly indicates otherwise, and it should also be understood that when the terms "comprise" and / or "include" are used in the specification, there is a feature, step, work, device, component and / or combination thereof.

[0096] It should be noted that the terms "first", "second" and the like in the specification and claims of the present application and the above-described drawings are used to distinguish similar objects, and do not necessarily indicate a specific order or sequence. It should be understood that the data used in this way can be interchanged under appropriate circumstances, so that the embodiments of the present application described herein can be implemented in an order other than those illustrated or described herein.

[0097] The above is only a preferred embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A cassette library preparation system, characterized in that: The invention comprises a narrow housing, wherein a gun discharge module is disposed in the narrow housing, a reaction module and a control module are disposed on both sides of the gun discharge module, the gun discharge module comprises a gantry support and a plurality of pipettes, each pipette corresponding to a group of reaction process samples, the gantry support is provided with a first driving mechanism for driving the pipette to move vertically, and the lower end of the gantry support has a first notch for the reaction module to pass through; The control module is arranged on a fixed frame, and the fixed frame has a second gap for the reaction module to pass through; A second driving mechanism for driving the reaction module to move horizontally and linearly is provided in the narrow housing; a cartridge is provided on the reaction module, and a reagent tube is placed in the cartridge. Driven by the second driving mechanism, the reaction module moves horizontally so that the reagent tube in the cartridge is directly below the pipette gun; The reaction module is provided with a modular temperature control component for the reagent tube.

2. The cassette library preparation system according to claim 1, characterized in that: The reaction module is provided with a reagent kit, which has a tip replacement area and a cleaning area for replacing and cleaning the tip of a pipette.

3. The cassette library preparation system according to claim 1, characterized in that: The card box is provided with several sample slots that are physically isolated from each other, and the reagent tubes are placed independently in the sample slots; a cover plate is provided above the sample slot, and the cover plate is provided with openings corresponding to each reagent tube so that a pipette can operate the reagent tube through the opening.

4. The cassette library preparation system according to claim 3, characterized in that: One end of the cover is rotatably arranged on the box body of the card box through a pivot, and at least two first positioning blocks are provided on the box body, and the first positioning blocks are rotatably arranged on the box body through a pivot; when the cover is closed on the sample slot, the first positioning block can be rotated so that one end of the first positioning block is above the other end of the cover, thereby fixing the position of the cover.

5. The cassette library preparation system according to claim 3, characterized in that: A card plate positioning groove is provided between two adjacent sample grooves, and a first card groove corresponding to the card plate positioning groove is provided on the cover plate; a card plate is also included, and the card plate passes through the first card groove and is inserted into the card plate positioning groove to fix the position of the card plate.

6. The cassette library preparation system according to claim 3, characterized in that: The inner wall of the sample tank is sprayed with a hydrophobic coating, and the hydrophobic coating is a Pyroline coating with a contact angle greater than 120°.

7. The cassette library preparation system according to claim 1, characterized in that: The reaction module is equipped with a fluorescence scanning device for the reagent tubes.

8. The cassette library preparation system according to claim 1, wherein: The modular temperature control assembly includes a copper heating plate and a temperature zone insulation plate. The copper heating plate is provided with two groups of sleeves controlled by different temperature control systems. The sleeves are used to insert reagent tubes. The temperature zone insulation plate is provided with insulation holes for inserting the sleeves.

9. The cassette library preparation system according to claim 1, characterized in that: The liquid circuit module connected to the liquid transfer gun in the discharge gun module is an independent closed liquid circuit system, which is isolated from the electrical circuit space.

10. The cassette library preparation system according to claim 1, wherein: The first driving mechanism includes a bipolar transmission structure, including a first screw-nut transmission mechanism and a second screw-nut transmission mechanism. The first screw-nut transmission mechanism is arranged on the gantry bracket and its nut is connected to the first supporting plate. The second screw-nut transmission mechanism is arranged on the first supporting plate and its nut is connected to the second supporting plate. A support rod is fixedly provided on the second supporting plate, and the support rod is fixedly connected to the pipette gun.

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