Positive pressure head applied to 96-channel pipetting workstation

Abstract

The utility model belongs to the technical field of biological experiments, particularly relates to a positive pressure head applied to a 96-channel pipetting workstation, and aims to solve the problems that an existing positive pressure module is large in size and cannot be suitable for a small pipetting workstation, the following scheme is provided: the positive pressure head comprises a workstation main body, and the workstation main body comprises a pump head main body; the bottom of the pump head main body is connected with a plurality of pump head suction nozzles, and the pump head main body is matched with an external manipulator and is used for completing pipetting operation; the work station main body further comprises a suction head placing frame, a liquid storage box and a container placing frame, and the positive pressure head has the advantages that the volume is greatly reduced through the exquisite design, and the positive pressure head can be matched with a small pipetting work station; the positive pressure head pushes a reagent to be quickly filtered by using compressed gas, so that each liquid cavity is uniformly stressed, and the filtering efficiency is improved; by accurately regulating and controlling the gas flow and pressure and combining the micropore design, the gas is effectively prevented from leaking too fast, and the contradiction among the filtering speed, the gas source pressure and the flow is balanced.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of biological experiment technology especially, it relates to the positive pressure head applied to 96 channel pipette workstation. BACKGROUND

[0002] In the laboratory of the field such as biotechnology, drug research and development, environmental monitoring, 96 channel pipette workstation has become an important tool for automatic pipetting operation. Such workstation can efficiently complete a large number of sample reagent transfer, mixing and distribution and other tasks through accurate mechanical arm and pump head system. However, in the reagent transfer process, the existing pipette workstation still has the following problems:

[0003] At present, the existing pipette workstation mostly integrates filtering function, which often uses filter plate to filter reagent, in order to cooperate with the filter plate, the existing pipette workstation mostly uses positive pressure module to assist filtering, but the positive pressure module is usually large in size, which is mostly placed in large pipette workstation, cooperates with each other and works together, cannot be applied to small pipette workstation, and the applicability is poor.

[0004] In view of the above problems, the utility model file proposes the positive pressure head applied to 96 channel pipette workstation. UTILITY MODEL CONTENT

[0005] The utility model aims at solving the shortcomings that the positive pressure module is large in size and cannot be applied to small pipette workstation in the prior art, and proposes the positive pressure head applied to 96 channel pipette workstation.

[0006] In order to achieve the above object, the utility model adopts the following technical scheme:

[0007] The positive pressure head applied to 96 channel pipette workstation includes:

[0008] The workstation main body includes pump head main body, the bottom of the pump head main body is connected with a plurality of pump head suction nozzles, the pump head main body cooperates with external mechanical hand and is used for completing pipetting operation;

[0009] The workstation main body further includes suction head placing rack, liquid storage tank and container placing rack, a plurality of liquid taking suction heads are placed in the inside of the suction head placing rack, a plurality of the liquid taking suction heads are used to take reagent, the liquid storage tank is used to store reagent to be transferred, a plurality of containers are placed in the inside of the container placing rack and are used to independently store reagent after transfer;

[0010] Further include filter plate, the filter plate is arranged above the container placing rack, and the filter plate is used to filter reagent after completing transfer;

[0011] The positive pressure head is used to assist the filter plate to complete the sufficient filtration of the internal reagent.

[0012] In a possible design, the filter plate comprises a plurality of liquid cavities opened inside the filter plate, one end of each of the plurality of liquid cavities penetrates through the top of the filter plate, the bottom end of each of the plurality of liquid cavities is fixedly connected with a liquid outlet pipe, each of the plurality of liquid outlet pipes is matched with a plurality of containers in the container placing rack, the inside of each of the plurality of liquid cavities is sealingly provided with a filter membrane, and the plurality of filter membranes are used to complete the filtration of the reagent.

[0013] In a possible design, the top of the positive pressure head is provided with a plurality of clamping holes matched with a plurality of pump head nozzles at the bottom of the pump head body, so as to ensure that the pump head body can complete the taking of the positive pressure head.

[0014] In a possible design, the inside of the positive pressure head is provided with a plurality of air cavities in communication, one side of the positive pressure head is provided with an air inlet in communication with the plurality of air cavities, the air inlet is connected with an external air supply device, so as to complete the delivery of the gas, the bottom of each of the plurality of air cavities is provided with a micropore matched with a liquid cavity, and the plurality of micropores are used to complete the discharge of the gas.

[0015] In a possible design, the bottom of the positive pressure head is provided with a rubber pad, the inside of the rubber pad is provided with a plurality of through holes matched with the micropores, and the rubber pad is used to ensure that the air tightness is good when the filter plate and the positive pressure head are connected.

[0016] In a possible design, the workstation body further comprises a carrier plate, the top of the carrier plate is provided with four object placing grooves, and the four object placing grooves are used to sequentially place the pump head placing rack, the liquid storage tank, the container placing rack and the positive pressure head.

[0017] In the application, when used, the user can set the filter plate above the container placing rack on which a plurality of containers are placed, and simultaneously connect an external air supply device with the gas inlet of the positive pressure head by a pipeline; then start the device, and the workstation can drive the pump head body to move above the suction head placing rack, then the pump head body is lowered, a plurality of liquid taking suction heads can be taken up by the plurality of pump head nozzles, then the pump head body moves above the liquid storage tank, and the reagent in the liquid storage tank can be drawn into the plurality of liquid taking suction heads by cooperation of the pump head body and the plurality of liquid taking suction heads; then the workstation can drive the pump head body to move the plurality of liquid taking suction heads with the reagent to above the filter plate, then the plurality of liquid taking suction heads can be inserted into the corresponding liquid cavities and complete the discharge of the reagent, and the reagent is stopped in the plurality of liquid cavities by the blockage of the filter membrane; after completing the liquid discharge, the plurality of liquid taking suction heads can be placed back into the suction head placing rack by the pump head body, then the pump head body can move above the positive pressure head, the positive pressure head can be taken up by cooperation of the plurality of pump head nozzles and the clamping holes, and the positive pressure head can move synchronously with the pump head body to above the filter plate; then the pump head body can drive the positive pressure head to be lowered and stacked above the filter plate, at this time, the rubber pad at the bottom of the positive pressure head can ensure good sealing effect between the positive pressure head and the filter plate; then the external air supply device can introduce compressed gas into the positive pressure head through the gas inlet, the compressed gas can enter the plurality of gas cavities through the gas inlet, and act on the liquid cavities through the micropores of the positive pressure head, at this time, the reagent in the liquid cavities can pass through the filter membrane under the action of pressure to realize filtration.

[0018] Beneficial effects: in the utility model, the positive pressure head applied to the 96-channel liquid transfer workstation is designed carefully, which significantly reduces the volume of the positive pressure module; the positive pressure head can be easily adapted to small liquid transfer workstations, breaking the limitation that the traditional positive pressure module cannot be applied in small workstations due to its large volume;

[0019] In the utility model, the positive pressure head applied to the 96-channel liquid transfer workstation provides compressed gas to the liquid cavities in the filter plate, and the gas pressure is used to push the reagent to quickly pass through the filter membrane, which significantly improves the filtration efficiency; at the same time, the design of the positive pressure head ensures that each liquid cavity can be subjected to uniform pressure, which guarantees the stability of the filtration effect;

[0020] In the utility model, the positive pressure head applied to the 96-channel liquid transfer workstation accurately controls the flow and pressure of the gas, which ensures that the reagent in each liquid cavity can be fully filtered; in addition, by reasonably setting the pore size of the micropore, it can be ensured that while providing enough pressure to push the reagent to filter, the loss of system pressure caused by too fast leakage of the gas is avoided; even if a certain liquid cavity is filtered first, due to the limiting effect of the micropore, the amount of gas leakage is very small, which will not significantly affect the gas cavity pressure, thereby ensuring the continued filtration of the reagent in other liquid cavities, effectively reducing the reagent residue, balancing the contradiction between the filtration speed, gas source pressure and gas source flow, and enhancing the stability of the entire system;

[0021] The positive pressure head can be adapted to a small pipetting workstation through the exquisite design of greatly reducing the volume; the positive pressure head utilizes compressed gas to push reagents for rapid filtration, ensures that each liquid cavity is uniformly stressed, and improves the filtration efficiency; through precise control of gas flow and pressure, combined with the design of micropores, rapid gas leakage is effectively prevented, the system pressure is maintained stable, sufficient filtration is ensured and reagent residue is reduced, the contradiction between filtration speed, gas source pressure and flow is balanced, and the stability of the system as a whole is enhanced. BRIEF DESCRIPTION OF DRAWINGS

[0022] Figure 1 The utility model provides a positive pressure head's workstation overall structure schematic drawing of application on 96 channel pipetting workstation;

[0023] Figure 2 The utility model provides a positive pressure head's workstation carrier plate structure schematic drawing of application on 96 channel pipetting workstation;

[0024] Figure 3 The utility model provides a positive pressure head's liquid taking suction head and its rack structure schematic drawing of application on 96 channel pipetting workstation;

[0025] Figure 4 The utility model provides a positive pressure head's workstation pump head structure schematic drawing of application on 96 channel pipetting workstation;

[0026] Figure 5 The utility model provides a positive pressure head's filter plate internal structure schematic drawing of application on 96 channel pipetting workstation;

[0027] Figure 6 The utility model provides a positive pressure head's positive pressure head top structure schematic drawing of application on 96 channel pipetting workstation;

[0028] Figure 7 The utility model provides a positive pressure head's positive pressure head cut structure schematic drawing of application on 96 channel pipetting workstation;

[0029] Figure 8 The utility model provides a positive pressure head's positive pressure head bottom structure schematic drawing of application on 96 channel pipetting workstation.

[0030] In the drawing: 1, carrier plate; 2, suction head rack; 3, liquid storage tank; 4, container rack; 5, filter plate; 6, positive pressure head; 7, pump head main body; 8, storage groove; 9, liquid taking suction head; 10, pump head suction nozzle; 11, liquid cavity; 12, liquid outlet pipe; 13, filter membrane; 14, clamping hole; 15, gas inlet; 16, gas cavity; 17, micropore; 18, rubber pad. DETAILED DESCRIPTION

[0031] The technical solutions in the embodiments of the utility model will be apparently and completely described in connection with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments.

[0032] Embodiment 1: Refer to Figures 1-8 A positive pressure head, comprising:

[0033] The workstation main body comprises a pump head main body 7, the bottom of the pump head main body 7 is fixed with a plurality of pump head nozzles 10 through a connecting piece, the pump head main body 7 cooperates with an external mechanical hand to complete a pipetting operation. The external mechanical hand takes the liquid taking nozzle 9 by using the pump head main body 7, and then takes the reagent through the liquid taking nozzle 9, and then transfers the reagent to the liquid cavity 11 of the filter plate 5.

[0034] The workstation main body further comprises a nozzle placing rack 2, a liquid storage tank 3 and a container placing rack 4, a plurality of liquid taking nozzles 9 are placed in the nozzle placing rack 2 in an orderly manner, and the plurality of liquid taking nozzles 9 are used to take the reagent. The liquid storage tank 3 is used to store the reagent to be transferred, and a plurality of containers are placed in the container placing rack 4, which is used to store the transferred reagent independently, so as to facilitate subsequent detection or analysis.

[0035] Further, the filter plate 5 is arranged above the container placing rack 4, and the filter plate 5 is used to filter the reagent after the transfer is completed. The arrangement of the filter plate 5 can realize the filtering treatment of the reagent, remove the impurities in the reagent, and improve the purity of the reagent.

[0036] Further, the positive pressure head 6 is used to assist the filter plate 5 to complete the sufficient filtration of the reagent in the filter plate 5. The arrangement of the positive pressure head 6 can provide additional pressure, so that the reagent can pass through the filter membrane 13 better, and the filtration efficiency is improved.

[0037] Further, in the embodiment, the filter plate 5 comprises a plurality of liquid cavities 11 arranged in the filter plate 5, one end of the plurality of liquid cavities 11 penetrates through the top of the filter plate 5, and is used to receive the reagent transferred from the liquid taking nozzle 9. The bottom end of the plurality of liquid cavities 11 is fixedly connected with a liquid outlet pipe 12, and the plurality of liquid outlet pipes 12 are matched with the plurality of containers in the container placing rack 4, and are used to discharge the filtered reagent into the corresponding containers. The inside of the plurality of liquid cavities 11 is sealingly provided with a filter membrane 13, and the material of the filter membrane 13 can be selected according to actual needs, such as polytetrafluoroethylene, nylon, etc. The plurality of filter membranes 13 are used to complete the filtration of the reagent and remove the impurities in the reagent.

[0038] Further, in the embodiment, the top of the positive pressure head 6 is provided with a plurality of clamping holes 14, and the plurality of clamping holes 14 are matched with the plurality of pump head nozzles 10 at the bottom of the pump head body 7. When it is needed to remove the positive pressure head 6 from the filter plate 5, the external mechanical hand can clamp the positive pressure head 6 from the filter plate 5 through the cooperation of the pump head body 7, the pump head nozzle 10 and the clamping hole 14, so as to ensure that the pump head body 7 can complete the taking of the positive pressure head 6, and realize the quick replacement or cleaning of the positive pressure head 6.

[0039] Further, in the embodiment, the inside of the positive pressure head 6 is provided with a plurality of air cavities 16, the plurality of air cavities 16 are communicated, and form an integrated air pressure cavity. One side of the positive pressure head 6 is provided with an air inlet 15, and the air inlet 15 is communicated with the plurality of air cavities 16. The air inlet 15 is connected with an external air supply device, and is used for completing the delivery of gas. The bottom of each of the plurality of air cavities 16 is provided with a micropore 17, each of the plurality of micropores 17 is matched with a liquid cavity 11, and the plurality of micropores 17 are used for completing the discharge of gas, so as to assist the filtration of reagents.

[0040] The application can be used in the field of biological experiment technology, and can also be used in other fields applicable to the application.

[0041] Embodiment 2: Reference Figure 1 、 2 , 8, on the basis of embodiment 1: the positive pressure head applied to the 96-channel pipetting workstation is applied to the field of biological experiment technology;

[0042] Further, in the embodiment, the bottom of the positive pressure head 6 is provided with a rubber pad 18, and the rubber pad 18 can be made of silica gel, rubber or other elastic materials. The inside of the rubber pad 18 is provided with a plurality of through holes matched with the micropores 17, which are used to ensure that the gas can be discharged from the air cavity 16 to the liquid cavity 11 through the micropore 17 and the through hole. The setting of the rubber pad 18 can ensure the good air tightness when the filter plate 5 and the positive pressure head 6 are connected, and avoid gas leakage.

[0043] Further, in the embodiment, the workstation body further includes a carrier plate 1, and the top of the carrier plate 1 is provided with four storage grooves 8, which are respectively used for sequentially placing the suction head placing rack 2, the liquid storage tank 3, the container placing rack 4 and the positive pressure head 6. Such a setting can make the entire workstation body more tidy and orderly, and also facilitate the transfer and filtration operation of the reagents by the operator.

[0044] Similarly, the embodiment can be designed into a positive pressure head with 24, 48 or other channel numbers for use in a small pipetting workstation. Similarly, it can also be designed into a positive pressure head with 12 plate positions, 24 plate positions or other different plate position numbers.

[0045] The working principle and use process of the technical solution are as follows: in use, the user can set the filter plate 5 above the container placing rack 4 where a plurality of containers are placed, and simultaneously connect an external air supply device with the gas inlet 15 of the positive pressure head 6 by a pipeline; then start the device, the workstation can drive the pump head body 7 to move above the suction head placing rack 2, then the pump head body 7 is lowered, a plurality of liquid taking suction heads 9 can be taken up by the plurality of pump head nozzles 10, then the pump head body 7 moves above the liquid storage tank 3, and the reagent in the liquid storage tank 3 can be drawn into the plurality of liquid taking suction heads 9 by cooperation of the pump head body 7 and the plurality of liquid taking suction heads 9; then the workstation can drive the pump head body 7 to move the plurality of liquid taking suction heads 9 that have taken the reagent above the filter plate 5, then the plurality of liquid taking suction heads 9 can be inserted into the corresponding liquid cavities 11 and complete the discharge of the reagent, and the reagent is blocked by the filter membrane 13 and stays in the plurality of liquid cavities 11; after completing the liquid discharge, the pump head body 7 can place the plurality of liquid taking suction heads 9 back into the suction head placing rack 2, then the pump head body 7 can move above the positive pressure head 6, the positive pressure head 6 can be taken up by cooperation of the plurality of pump head nozzles 10 and the clamping holes 14, and the positive pressure head 6 can move synchronously with the pump head body 7 above the filter plate 5; then the pump head body 7 can drive the positive pressure head 6 to descend and stack above the filter plate 5, at this time, the rubber pad 18 at the bottom of the positive pressure head 6 can ensure good sealing effect between the positive pressure head 6 and the filter plate 5; then the external air supply device can introduce compressed gas into the positive pressure head 6 through the gas inlet 15, the compressed gas can enter the plurality of gas cavities 16 through the gas inlet 15, and act on the liquid cavities 11 through the micropores 17 of the positive pressure head 6, at this time, the reagent in the liquid cavities 11 can pass through the filter membrane 13 under the action of pressure to realize filtration.

[0046] However, the filtration of 96 reagents is not synchronized, and there must be first and later; because the filter membrane 13 has a small blocking effect on gas, the liquid cavity 11 that completes filtration first loses the blocking of the reagent to the gas, and the gas will penetrate the filter membrane 13 to leak, at this time, the flow limiting effect of the micropore 17 is realized, only a small amount of gas will leak, which will not affect the pressure of the gas cavity 16, and further will not affect the pressure in the other liquid cavities 11 that have not completed filtration, and the filtration of all reagents in the liquid cavities 11 can be completed.

[0047] Only a gas flow slightly larger than the volume of the reagent is needed in each liquid cavity 11 to complete the filtration, so the flow limiting effect of the micropore 17 will not affect the normal filtration; if there is no micropore 17 design, a large amount of gas will leak through the liquid cavity 11 that completes filtration first, causing the pressure in the gas cavity 16 to be lost, and the filtration of the reagent in the remaining liquid cavities 11 cannot be completed, and the reagent remains in the liquid cavities 11, and the filtration fails.

[0048] The positive pressure head 6 body can balance the contradiction between the filtration speed, the gas source pressure and the gas source flow by reasonably designing the pore size of the micropore 17.

[0049] The above merely describes a preferred embodiment of the present application, and the protection scope of the present application is not limited thereto, and any skilled person in the art, according to the technical scheme and the inventive concept of the present application, makes equivalent replacement or change within the technical range disclosed by the present application, which should be covered within the protection scope of the present application.

Claims

1. A positive pressure head for use on a 96 channel pipetting station, characterised in that, Include: The workstation body includes a pump head body (7), the bottom of the pump head body (7) is connected with a plurality of pump head nozzles (10), the pump head body (7) is matched with an external mechanical hand, which is used for completing a pipetting operation; The workstation body also includes a tip holder (2), a liquid storage tank (3), and a container holder (4). The tip holder (2) has a plurality of liquid taking tips (9) placed inside, which are used for taking reagents. The liquid storage tank (3) is used for storing the reagents to be transferred. The container holder (4) has a plurality of containers placed inside, which are used for independently storing the transferred reagents. It also includes a filter plate (5) arranged above the container holder (4), which is used for filtering the reagents after the transfer is completed. It also includes a positive pressure head (6) for assisting the filter plate (5) to complete the filtration of the reagents inside.

2. Positive pressure head for use on a 96-channel pipetting station according to claim 1, characterized in that The filter plate (5) includes a plurality of liquid chambers (11) opened inside, one end of each of the plurality of liquid chambers (11) penetrates through the top of the filter plate (5), the bottom end of each of the plurality of liquid chambers (11) is fixedly connected with a liquid outlet pipe (12), the plurality of liquid outlet pipes (12) are matched with the plurality of containers in the container holder (4), a filter membrane (13) is sealingly arranged inside each of the plurality of liquid chambers (11), and the plurality of filter membranes (13) are used to complete the filtration of the reagents.

3. Positive pressure head for use on a 96-channel pipetting station according to claim 2, characterized in that The top of the positive pressure head (6) is provided with a plurality of clamping holes (14), and the plurality of clamping holes (14) are matched with the plurality of pump head nozzles (10) at the bottom of the pump head body (7), which can ensure that the pump head body (7) can complete the taking of the positive pressure head (6).

4. Positive pressure head for use on a 96-channel pipetting station according to claim 3, characterized in that The positive pressure head (6) has a plurality of air chambers (16) opened inside, the plurality of air chambers (16) are connected in communication, one side of the positive pressure head (6) is provided with an air inlet (15), the air inlet (15) is in communication with the plurality of air chambers (16), the air inlet (15) is connected with an external gas supply device, which is used for completing the delivery of gas, the bottom of each of the plurality of air chambers (16) is provided with a micropore (17), the plurality of micropores (17) are matched with the plurality of liquid chambers (11), and the plurality of micropores (17) are used to complete the discharge of gas.

5. Positive head for use on a 96-channel pipetting station according to claim 4, characterized in that The bottom of the positive pressure head (6) is provided with a rubber pad (18), a plurality of through holes matched with the micropores (17) are opened in the rubber pad (18), and the rubber pad (18) is used to ensure good air tightness when the filter plate (5) and the positive pressure head (6) are connected.

6. Positive pressure head for use on a 96-channel pipetting station according to claim 1, characterized in that The workstation body also includes a carrier plate (1), and the top of the carrier plate (1) is provided with four storage grooves (8) for sequentially placing the tip holder (2), the liquid storage tank (3), the container holder (4), and the positive pressure head (6).

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