Uniform mixing structure for preparing NK (Natural Killer) cells

Abstract

The utility model discloses a uniform mixing structure for preparing NK (Natural Killer) cells, and aims to provide the uniform mixing structure for preparing the NK cells, which has the advantages of improving the operation convenience, protecting a container and improving the uniform mixing preparation efficiency. The device comprises a base, the base is connected with vibration equipment, a supporting rod and a placing assembly, one end of the supporting rod is connected with the vibration equipment, the other end of the supporting rod is rotationally connected with a rotating disc, the rotating disc is connected with a plurality of supporting rods, the supporting rods are connected with clamping seats, and the clamping seats are provided with adjusting rings and a plurality of clamping blocks and inserting holes. The clamping blocks are rotationally connected with the inserting holes through the adjusting rings, one end of the containing assembly is rotationally connected with the base, and a plurality of containing grooves are formed in the other end of the containing assembly. The utility model has the beneficial effects that the uniform mixing structure is convenient for batch operation; the NK cell preparation efficiency is improved; the stability and protection of container operation are improved; and the structural connection stability is improved, and smooth clamping operation is guaranteed.

Description

TECHNICAL FIELD

[0001] The utility model relates to NK cell preparation technical field, especially point to a kind of mixing structure for NK cell preparation. BACKGROUND

[0002] Natural killer cell, namely NK cell, is important immune cell of organism, not only related to antitumor, antiviral infection and immunoregulation, and in certain circumstances, participate in hypersensitivity and the occurrence of autoimmune disease, single nuclear cell is separated from peripheral blood, after in-vitro activation, expansion is back to patient's body, enhances patient's immune function or directly kills tumor cell and virus infection cell, can effectively inhibit tumor cell metastasis and diffusion, so the demand preparation of NK cell is very crucial in medical field, including cell mixing in preparation, mixing of NK cell is a very important link in cell experiment, and the mixing method of existing cell is generally mixed by mixing device.

[0003] Chinese patent authorized announcement number: CN 221244878 U, authorized announcement date is July 2, 2024, the utility model relates to a kind of immune cell mixing device, relate to experimental tool technical field, including: base, vertical rod is set to the top of base, vertical rod outer end both sides are provided with two plates, plate top is provided with placing hole, clamping component is set to the inside of placing hole, for clamping and fixing test tube and test bottle, fixed component is set to the bottom of base, for the fixation of base.The technical scheme has the following disadvantages: 1. when fixing container, one-way extrusion is fixed by abutment plate, so that the stress of container is uneven, and the container is easily damaged, which leads to mixing operation failure;2. screw rod is small as operation target, difficult to operate conveniently, reduce mixing efficiency;3. when batch operation preparation, the number of preparation is limited, and the container is time-consuming and laborious, which further affects the preparation efficiency.

[0004] In summary: mixing device has the disadvantages of inconvenient operation, easy to damage container and reduce the preparation efficiency of mixing. UTILITY MODEL CONTENT

[0005] The utility model is to overcome the inconvenience of operation of mixing device in the prior art, easy to damage container and reduce the preparation efficiency of mixing, provide a kind of mixing structure for NK cell preparation, which improves the operation convenience, protects container and improves the preparation efficiency of mixing.

[0006] In order to achieve the above purpose, the utility model adopts the following technical scheme:

[0007] The utility model provides a kind of mixing structure for NK cell preparation, including base, which is connected with vibration equipment, support rod and placing assembly, one end of support rod is connected with vibration equipment, the other end of support rod is rotatably connected with rotating disc, rotating disc is connected with several support bars, support bar is connected with clamping seat, clamping seat is equipped with adjusting ring and several clamping blocks, clamping seat is equipped with insertion hole, clamping block is rotatably connected with insertion hole by adjusting ring, one end of placing assembly is rotatably connected with base, the other end of placing assembly is equipped with several placing grooves.

[0008] Vibration equipment is installed on base, support rod is connected with vibration equipment to vibrate support rod, rotating disc is connected on support rod, rotating disc is circular plate structure, several support bars are evenly connected on the circumference of rotating disc to facilitate batch operation of NK cell mixing preparation;Clamping seat is connected on the other end of support bar, clamping seat clamps container by clamping block evenly arranged on insertion hole, each clamping block evenly arranged simultaneously forces container by adjusting ring, so that the stability and stress of container are uniform, thereby improving stability and preventing container from breaking;Placing assembly is connected on base to quickly load container on clamping seat and place container to further improve NK cell preparation efficiency.

[0009] As preferred, the hole wall of the insertion hole is provided with mounting holes, the mounting holes are arranged evenly around the insertion hole, the clamping block includes a connecting rod, one end of the connecting rod is provided with a rotating hole, the mounting hole is connected with a rotating rod, and the rotating rod is inserted into the rotating hole. The mounting hole is evenly opened on the clamping seat and communicates with the insertion hole, so that the connecting rod in the clamping block is connected by the rotating rod as a fulcrum to rotate in the mounting hole, so as to stably clamp and unlock the container inserted into the insertion hole. It achieves the effect of ensuring the stability and smoothness of the structure connection and clamping locking and unlocking.

[0010] As preferred, the clamping block includes a top block, one side of the other end of the connecting rod is connected with the top block, the top block is provided with a guide slope one, the clamping seat is provided with a sliding hole, a top rod is inserted into the sliding hole, one end of the top rod is slidably connected with the guide slope one, the other end of the top rod is connected with the adjusting ring, and the adjusting ring is movably connected with the clamping seat. The top block in the clamping block is connected to the tail end of the rotating rod, and the surface of the top block is provided with a guide slope one, so as to drive the top rod to move by the movement of the adjusting ring, and then the top rod pushes the top block and the connecting rod inward along the guide slope one, realizes clamping action, and the adjusting ring realizes synchronous clamping operation by simultaneously driving each clamping block. It achieves the effect of improving the stability of structure connection and ensuring the smoothness of clamping operation.

[0011] As preferred, the clamping seat is provided with a connecting groove, one end of the adjusting ring connected with the connecting groove is threadedly connected, the other end of the adjusting ring is connected with a snap ring, the top rod is provided with a limiting groove, and the snap ring is clamped with the limiting groove. The clamping seat is provided with a connecting groove, the connecting groove is an annular recess, the adjusting ring is displaced up and down in the clamping seat through the threaded connection with the connecting groove, and then the connected snap ring drives the top rod to move up and down to complete the pushing and relaxation of the top block. The effect of convenient adjustment and improved operation efficiency is achieved.

[0012] As preferred, the other side of the other end of the connecting rod is connected with a buffer block, and the buffer block is provided with a guide inclined surface two. The buffer block is connected to the side surface of the opposite end of each connecting rod, and the buffer block is made of soft rubber material, so that the contact-locked container can be clamped by strong friction force while protecting the container. The effects of further improving the stability of the container fixing and protecting the container are achieved.

[0013] As preferred, the placing assembly comprises a bottom disc and a rotating seat, one end of the bottom disc is connected with the base, the other end of the bottom disc is connected with a rotating column, one end of the rotating seat is inserted with the rotating column, and a placing groove is arranged at the other end of the rotating seat and matched with the position of the insertion hole. The bottom disc in the placing assembly is connected with the base to support the rotating seat, so that the rotating seat can rotate the placing groove to below the insertion hole through the connection of the rotating column, each container can be quickly placed through the placing groove, and the container can be quickly unlocked and placed into the placing groove by rotating the placing groove and the insertion hole to be opposite. The effects of further improving the mixing preparation efficiency of NK cells are achieved.

[0014] The beneficial effects of the utility model are: the mixing structure is convenient for batch operation; the NK cell preparation efficiency is improved; the stability and protection of the container operation are improved; the structural connection stability is improved, and the clamping operation is smooth. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 is the perspective view of the utility model;

[0016] Figure 2 is the structural schematic view of the rotating disc, the clamping seat and the clamping block;

[0017] Figure 3 is the connecting sectional view of the rotating disc, the clamping seat and the clamping block;

[0018] Figure 4 is the sectional view of the placing assembly.

[0019] In the figure: 1. base, 2. vibration device, 3. support rod, 4. placing assembly, 5. rotating disc, 6. support rod, 7. clamping seat, 8. adjusting ring, 9. clamping block, 10. insertion hole, 11. placing groove, 12. mounting hole, 13. connecting rod, 14. rotating hole, 15. rotating rod, 16. top block, 17. guiding inclined surface I, 18. sliding hole, 19. top rod, 20. connecting groove, 21. clamping ring, 22. limiting groove, 23. buffer block, 24. base plate, 25. rotating seat, 26. rotating column, 27. anti-skid block, 28. buffer pad. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments of the present application. The description of the at least one exemplary embodiment is actually only illustrative, but not as any limitation on the present application and its application or use. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without any creative work are within the scope of protection of the present application.

[0021] It should be noted that the terms used herein are only for describing specific embodiments, and are not intended to limit the exemplary embodiments according to the present application. As used herein, the singular form is intended to include the plural form, unless the context clearly indicates otherwise, and it should also be understood that when the terms "comprise" and / or "include" are used in the specification, there is a feature, step, operation, device, component and / or combination thereof.

[0022] Unless otherwise specified, the relative arrangement of components, numerical expressions, and values ​​described in these embodiments do not limit the scope of this application. For ease of description, spatially relative terms such as "upper," "lower," "left," and "right" are used in the embodiments to illustrate the relationship of one element or feature shown in the figures to another. It should be understood that, in addition to the orientations shown in the figures, spatial terms are intended to include different orientations of the device during use or operation. For example, if the device in the figure is inverted, an element described as being "below" another element or feature would be positioned "above" the other element or feature. Therefore, the exemplary term "below" can include both upper and lower orientations. The device can be positioned in other ways (rotated 90 degrees or in other orientations), and the spatially relative descriptions used herein should be interpreted accordingly. It should also be understood that, for ease of description, the dimensions of the various parts shown in the drawings are not drawn to scale. Technologies, processes, and equipment known to those skilled in the relevant art may not be discussed in detail, but, where appropriate, should be considered part of the specification. In all examples shown and discussed herein, any specific values ​​should be interpreted as merely exemplary and not limiting. Therefore, other examples of the exemplary embodiments may have different values.It should be noted that like reference numerals and letters represent similar items in the following figures, and therefore, once an item is defined in one figure, it need not be further discussed in subsequent figures.

[0023] In addition, it should be noted that the use of terms such as "first" and "second" to limit components is only for the convenience of distinguishing the corresponding components. Unless otherwise stated, the above terms have no special meaning and therefore cannot be understood as limiting the scope of protection of this application. Example 1:

[0024] like Figure 1 As shown, a mixing structure for preparing NK cells includes a base 1, the base 1 is connected to a vibration device 2, a support rod 3 and a placement component 4, one end of the support rod 3 is connected to the vibration device 2, and the other end of the support rod 3 is rotatably connected to a rotating disk 5, the rotating disk 5 is connected to a plurality of support rods 6, and the support rods 6 are connected to a clamping seat 7, the clamping seat 7 is provided with an adjustment ring 8 and a plurality of clamping blocks 9, the clamping seat 7 is provided with a socket 10, the clamping block 9 is rotatably connected to the socket 10 through the adjustment ring 8, one end of the placement component 4 is rotatably connected to the base 1, and the other end of the placement component is provided with a plurality of placement slots 11.

[0025] like Figure 2 As shown, the hole wall of the socket 10 is provided with a mounting hole 12, and there are several mounting holes 12 and they are evenly arranged around the socket 10. The clamping block 9 includes a connecting rod 13, and one end of the connecting rod 13 is provided with a rotating hole 14. The mounting hole 12 is connected to a rotating rod 15, and the rotating rod 15 is plugged into the rotating hole 14.

[0026] As Figure 2 , 3 shown, the clamping block 9 includes a top block 16, one side of the other end of the connecting rod 13 is connected with the top block 16, the top block 16 is provided with a guide slope 17, the clamping seat 7 is provided with a sliding hole 18, the sliding hole 18 is inserted with a top rod 19, one end of the top rod 19 is slidably connected with the guide slope 17, the other end of the top rod 19 is connected with the adjusting ring 8, the adjusting ring 8 is movably connected with the clamping seat 7.

[0027] As Figure 2 , 3 shown, the clamping seat 7 is provided with a connecting groove 20, one end connected with the adjusting ring 8 is threadedly connected with the connecting groove 20, the other end of the adjusting ring 8 is connected with a snap ring 21, the top rod 19 is provided with a limiting groove 22, the snap ring 21 is clamped with the limiting groove 22. The other side of the other end of the connecting rod 13 is connected with a buffer block 23, the buffer block 23 is provided with a guide slope 2.

[0028] As Figure 1 , 4 shown, the placing assembly 4 includes a bottom disc 24 and a rotating seat 25, one end of the bottom disc 24 is connected with the base 1, the other end of the bottom disc 24 is connected with a rotating column 26, one end of the rotating seat 25 is inserted with the rotating column 26, the placing groove 11 is placed at the other end of the rotating seat 25, the placing groove 11 is matched with the position of the insertion hole 10.

[0029] As Figures 1-4 shown: the adjusting ring 8 is connected with an anti-skid block 27. By increasing the friction, preventing the slip when rotating the adjusting ring 8.

[0030] The placing groove 11 is connected with a buffer pad 28, the buffer pad 28 is made of soft material to protect the equipment.

[0031] In use: the container containing the NK cells to be mixed and prepared is loaded in the placing groove 11 of the rotating seat 25, the container is taken out one by one and inserted into the insertion hole 10 for fixation, during the fixation of the container, the adjusting ring 8 is threadedly lowered in the connecting groove 20 by rotating the adjusting ring 8, thereby driving the snap ring 21 connected with the adjusting ring 8, the snap ring 21 drives the top rod 19 to descend, the top block 16 on each clamping block 9 is subjected to the downward pushing force of the top rod 19, the top block 16 pushes the connecting rod 13 to drive the buffer block 23 to move to the center of the insertion hole 10, thereby gradually clamping and fixing the container to prevent the movement of the container. Start the vibration device to shake the container to achieve mixing, and complete the preparation of the NK cells. After the mixing is completed, the clamping block 9 is loosened by reversing the rotation of the adjusting ring 8, and the container can be taken out one by one and placed back into the placing groove 11 below.

[0032] The above examples are only used to illustrate the technical solutions of the present application, and are not limiting; although the present application has been described in detail with reference to the foregoing examples, those skilled in the art should understand that the technical solutions recorded in the foregoing examples can still be modified, or some technical features therein can be replaced by equivalents; and these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the present application.

Claims

1. A mixing structure for the preparation of NK cells, characterized in that, The utility model provides a kind of vibration device, including base (1), the base (1) is connected with vibration device (2), support rod (3) and placing assembly (4), one end of support rod (3) is connected with vibration device (2), the other end of support rod (3) is rotatably connected with rotating disc (5), rotating disc (5) is connected with several support bars (6), support bar (6) is connected with clamping seat (7), clamping seat (7) is equipped with adjusting ring (8) and several clamping blocks (9), clamping seat (7) is equipped with insertion hole (10), clamping block (9) is rotatably connected with insertion hole (10) by adjusting ring (8), one end of placing assembly (4) is rotatably connected with base (1), and the other end of placing assembly is equipped with several placing grooves (11).

2. The mixing structure for preparing NK cells according to claim 1, wherein The hole wall of the insertion hole (10) is provided with a mounting hole (12), the mounting hole (12) is provided with a plurality of and evenly arranged around the insertion hole (10), the clamping block (9) includes a connecting rod (13), one end of the connecting rod (13) is provided with a rotating hole (14), the mounting hole (12) is connected with a rotating rod (15), and the rotating rod (15) is inserted into the rotating hole (14).

3. The mixing structure for preparing NK cells according to claim 2, wherein The clamping block (9) includes a top block (16), one side of the other end of the connecting rod (13) is connected with the top block (16), the top block (16) is provided with a guide inclined surface (17), the clamping seat (7) is provided with a sliding hole (18), the sliding hole (18) is inserted with a top rod (19), one end of the top rod (19) is slidably connected with the guide inclined surface (17), the other end of the top rod (19) is connected with the adjusting ring (8), and the adjusting ring (8) is movably connected with the clamping seat (7).

4. The mixing structure for preparing NK cells according to claim 3, wherein The clamping seat (7) is provided with a connecting groove (20), one end of the adjusting ring (8) connected with the connecting groove (20) is threadedly connected, the other end of the adjusting ring (8) is connected with a snap ring (21), the top rod (19) is provided with a limiting groove (22), and the snap ring (21) is clamped with the limiting groove (22).

5. The mixing structure for preparing NK cells according to claim 4, wherein The other side of the other end of the connecting rod (13) is connected with a buffer block (23), and the buffer block (23) is provided with a guide inclined surface (17).

6. The mixing structure for preparing NK cells according to claim 2, wherein The placing assembly (4) includes a bottom disc (24) and a rotating seat (25), one end of the bottom disc (24) is connected with the base (1), the other end of the bottom disc (24) is connected with a rotating column (26), one end of the rotating seat (25) is inserted into the rotating column (26), the placing groove (11) is arranged at the other end of the rotating seat (25), and the placing groove (11) is matched with the position of the insertion hole (10).

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