Multi-layer microbial strain culture device

Abstract

The utility model relates to the technical field of bioengineering, and discloses a multi-layer microbial strain culture device which comprises a combustion changing path, the left side of the outer wall of the combustion changing path is communicated with a concentrator, the left end of the concentrator is communicated with a first pipeline, the left end of the first pipeline is communicated with an electromagnetic valve, the left side of the electromagnetic valve is communicated with a check valve, and the left side of the check valve is communicated with a second pipeline. The left side of the check valve is communicated with a pressure reducing valve, the left side of the pressure reducing valve on the lower side is communicated with an air window, the left side of the pressure reducing valve on the upper side is communicated with an atomizer, the left side of the atomizer is communicated with a high-pressure water tank, and the left side of the pressure reducing valve on the middle side is communicated with an oxygen tank. According to the utility model, through a multi-layer structural design and a vertical stacking mode, the space overlapping of a plurality of culture units is realized, the culture capacity of a unit area is obviously improved, and meanwhile, the built-in temperature, humidity and CO2 concentration sensors and adjusting devices ensure the stability of a culture environment and meet the growth requirements of different microorganisms.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of bioengineering technology especially multilayer formula microorganism strain culture device. BACKGROUND

[0002] In the biological technology research and industrial production process, the culture of microorganism is a basic and key step. The traditional microorganism culture equipment is single layer structure, and the space utilization rate is low, and it is difficult to meet the requirements of high efficiency, energy saving and environmental protection in large-scale production, therefore, developing a kind of multilayer formula microorganism strain culture device which can improve the space utilization rate, save energy and be convenient to operate has important practical significance.

[0003] When the research personnel explore the physiological characteristics, metabolic mechanism and genetic law basic research of microorganism, need to cultivate various different types of microorganism strains, multilayer formula culture device can cultivate a plurality of microorganisms simultaneously, it is convenient to compare and study the growth difference of different strains under the same culture condition, provide rich data support for theoretical research, study the growth curve of different bacteria, the utilization of different nutrients.

[0004] In the laboratory or production site, space is limited and precious, lack of top design means that in the same land area or space volume, the culture container or culture area that can be placed reduces, cannot cultivate a large number of microorganism strains simultaneously, this is a serious limitation for the experiment, production or research project that needs to cultivate strains on a large scale, in the microorganism fermentation industry, unable to carry out large-scale strain culture can affect the subsequent fermentation production efficiency and yield. UTILITY MODEL CONTENTS

[0005] In order to make up for the above shortcomings, the utility model provides multilayer formula microorganism strain culture device, aims at improving the problem that a large number of microorganism strains cannot be cultivated simultaneously in prior art, which is a serious limitation for production or research project, and large-scale strain culture can affect the subsequent fermentation production efficiency and yield.

[0006] In order to realize the above purpose, the utility model adopts the following technical scheme: multilayer formula microorganism strain culture device, including the exchange combustion path, the outer wall left side of the exchange combustion path is connected with the concentrator, the left end of the concentrator is connected with the pipeline no.

[0007] As a further description of the above technical solutions:

[0008] The outer wall of the long plate is connected with a virtual pipeline at the bottom, and the right side of the long plate and the virtual pipeline is fixedly connected with a temperature and humidity instrument.

[0009] As a further description of the above technical solutions:

[0010] The outer wall of the long plate is connected with a virtual pipeline at the bottom, and the right side of the long plate and the virtual pipeline is fixedly connected with a temperature and humidity instrument.

[0011] As a further description of the above technical solutions:

[0012] The left side of the long plate is connected with a hole, and the bottom of the long plate is fixedly connected with an inclined plate.

[0013] As a further description of the above technical solutions:

[0014] The bottom of the inclined plate is fixedly connected with a drainage plate, and the top of the inclined plate is provided with a magnetic stirrer.

[0015] As a further description of the above technical solutions:

[0016] The right side of the long plate is provided with a microprocessor, and the front side of the microprocessor is fixedly connected with a controller.

[0017] As a further description of the above technical solutions:

[0018] The outer wall of the long plate is connected with a virtual pipeline at the bottom, and the right side of the long plate and the virtual pipeline is fixedly connected with a temperature and humidity instrument.

[0019] The utility model has the following beneficial effects:

[0020] 1、The utility model discloses a multi-layer microbial strain culture device, which comprises a vertical stack of multiple culture units, each culture unit comprising a culture chamber, a temperature and humidity sensor, a CO2 concentration sensor, a heating device, a cooling device, a CO2 injection device, a temperature and humidity adjusting device, a CO2 concentration adjusting device, a data acquisition and processing device, and a remote monitoring and control device.

[0021] 2、The utility model discloses a multi-layer microbial strain culture device, which comprises a vertical stack of multiple culture units, each culture unit comprising a culture chamber, a temperature and humidity sensor, a CO2 concentration sensor, a heating device, a cooling device, a CO2 injection device, a temperature and humidity adjusting device, a CO2 concentration adjusting device, a data acquisition and processing device, and a remote monitoring and control device. DRAWINGS

[0022] Figure 1 The utility model discloses a multi-layer microbial strain culture device, which comprises a vertical stack of multiple culture units, each culture unit comprising a culture chamber, a temperature and humidity sensor, a CO2 concentration sensor, a heating device, a cooling device, a CO2 injection device, a temperature and humidity adjusting device, a CO2 concentration adjusting device, a data acquisition and processing device, and a remote monitoring and control device.

[0023] Figure 2This is a microprocessor structure diagram of the multi-layer microbial strain cultivation device proposed by the present invention;

[0024] Figure 3 This is a diagram showing the long plate structure of the multi-layer microbial strain cultivation device proposed in the present invention;

[0025] Figure 4 This is a disassembled diagram of the long board of the multi-layer microbial strain cultivation device proposed in the present invention;

[0026] Figure 5 This is a schematic diagram of the inclined plate structure of the multi-layer microbial strain cultivation device proposed in the present invention.

[0027] Legend:

[0028] 1. Pipeline 1; 2. Hub; 3. Compressor; 4. Fuel exchange circuit; 5. Temperature and humidity meter; 6. Virtual pipeline; 7. Pipeline 2; 8. Collector; 9. Solenoid valve; 10. Check valve; 11. Pressure reducing valve; 12. Air window; 13. Air reduction floor; 14. Oxygen tank; 15. Carbon dioxide; 16. High-pressure water tank; 17. Atomizer; 18. Microprocessor; 19. Controller; 20. Long board; 21. Hole; 22. Magnetic stirrer; 23. Drain board; 24. Inclined board. DETAILED DESCRIPTION

[0029] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0030] Please see the attached Figure 1 - Attachment Figure 3 , the utility model provides an embodiment: a multi-layer microbial strain cultivation device, including a fuel exchange path 4, the left side of the outer wall of the fuel exchange path 4 is connected with a hub 2, the left end of the hub 2 is connected with a pipe 1, the left end of the pipe 1 is connected with a solenoid valve 9, the left side of the solenoid valve 9 is connected with a check valve 10, the left side of the check valve 10 is connected with a pressure reducing valve 11, the left side of the lower pressure reducing valve 11 is connected with an air window 12, the left side of the upper pressure reducing valve 11 is connected with an atomizer 17, the left side of the atomizer 17 is connected with a high-pressure water tank 16, the left side of the middle pressure reducing valve 11 is connected with an oxygen tank 14, the left side of the middle upper pressure reducing valve 11 is connected with carbon dioxide 15, the left side of the middle lower pressure reducing valve 11 is connected with a gas reduction ground 13, the right side of the fuel exchange path 4 is connected with a long plate 20, the left side of the long plate 20 is connected with a hole 21, and the bottom of the long plate 20 is fixedly connected with an inclined plate 24;

[0031] Specifically, through the left end of the pipeline 1 is cleverly connected with electromagnetic valve 9. Electromagnetic valve 9 as the key components, its left side is closely connected with check valve 10, to ensure the one-way flow of fluid, prevent the possible reverse flow phenomenon, check valve 10 left side is connected with pressure reducing valve 11, the role of pressure reducing valve 11 is to reduce the pressure of the fluid, to meet the operation requirements of subsequent equipment. The left side of pressure reducing valve 11, the fluid is distributed to different paths, respectively with air window 12, atomizer 17, carbon dioxide 15 and gas reduction ground 13, each device bears a specific function, air window 12 for the system to provide the necessary air power, atomizer 17 is responsible for the conversion of liquid into small particles, high pressure water tank 16 stores high pressure water for future use, oxygen tank 14 for providing the necessary oxygen, carbon dioxide 15 for adjusting chemical reaction, and gas reduction ground 13 to ensure smooth discharge of gas.

[0032] Please refer to the attached Figure 4 - attached Figure 5 , the outer wall of the lower end of the exchange fuel circuit 4 is connected with pipeline two 7, the bottom end of pipeline two 7 is connected with receiver 8, the outer wall of the bottom of long plate 20 is connected with virtual pipeline 6, the right side of long plate 20 and virtual pipeline 6 is fixedly connected with temperature and humidity instrument 5, the bottom of inclined plate 24 is fixedly connected with drainage plate 23, the top of inclined plate 24 is provided with magnetic stirrer 22, the right side of long plate 20 is provided with microprocessor 18, the front side of microprocessor 18 is fixedly connected with controller 19, the top of the outer wall of exchange fuel circuit 4 is connected with compressor 3;

[0033] Specifically, through the shape and size of these virtual pipeline 6 and long plate 20 matching, to ensure the stability and sealing of the overall structure, on the right side of long plate 20 and virtual pipeline 6, fixedly connected with a precise temperature and humidity instrument 5, the temperature and humidity instrument 5 can monitor and record the temperature and humidity change in the environment in real time, the bottom of inclined plate 24 is fixedly connected with a high efficiency drainage plate 23, the drainage plate 23 can quickly drain the excess moisture on the inclined plate 24, to ensure the dryness and cleanliness of inclined plate 24, on the top of inclined plate 24, a performance outstanding magnetic stirrer 22 is arranged, the magnetic stirrer 22 can uniformly mix the various substances on the inclined plate 24, and on the right side of long plate 20, a powerful microprocessor 18 is arranged, in order to ensure the normal work of microprocessor 18, a group of controllers 19 is fixedly connected to the front side of microprocessor 18, the controllers 19 can provide stable power supply for microprocessor 18.

[0034] Working principle: when the high oxygen environment E. coli culture and anaerobic gas production C. perfringens culture is needed, cooperate with E. coli culture medium, LBG culture medium, yeast powder 5g / L, sodium chloride 10g / L, glucose 5g / L, peptone 10g / L, liquid volume 25ml / 500ml, inoculation amount 1%, temperature 37℃, set all pressure reducing valve pressure to 0.2MPa, close the corresponding electromagnetic valve 9 for temperature and humidity instrument automatic control, start the corresponding electromagnetic valve 9 for 10 seconds, close for 50 seconds, cycle start and close, at the same time, start the corresponding electromagnetic valve 9 when the temperature is low, start the corresponding electromagnetic valve 9 when the temperature is high, control the temperature rise or fall, start the corresponding electromagnetic valve 9 for 10 seconds, close for 40 seconds, cycle start and close, at the same time, start the corresponding electromagnetic valve 9 when the temperature is low, start the corresponding electromagnetic valve 9 when the temperature is high, control the temperature rise or fall, cultivate for 24h, obtain OD600 bacteria amount 12.5, the same shaker obtains bacteria OD 10.3, gas production C. perfringens culture, culture medium: LBG culture medium, yeast powder 5g / L, sodium chloride 10g / L, glucose 5g / L, peptone 10g / L, liquid volume 25ml / 500ml, inoculation amount 1%, temperature 43℃, then the electromagnetic valve 9 is started for 20 seconds, at the same time, the electromagnetic valve 9 is started for 2 seconds and then closed, when to 22 seconds, the electromagnetic valve 9 is opened, the nitrogen fills the room, the cycle is started, at the same time, the electromagnetic valve 9 is started when the temperature is low, the electromagnetic valve 9 is started when the temperature is high, control the temperature rise or fall, cultivate for 24h, obtain OD 15.6, ordinary anaerobic culture device OD600 13.8.

[0035] Finally, it should be noted that: the above only for the preferred embodiments of the present application, and not for limiting the present application, although the present application has been described in detail with reference to the foregoing embodiments, for the person skilled in the art, it still can be modified to the technical scheme recorded in the foregoing embodiments, or equivalent replacement to part of the technical features, any modification, equivalent replacement, improvement, etc. within the spirit and principles of the present application, should be included in the protection scope of the present application.

Claims

1. A multi-layered microbial strain culture device comprising a burner replacement path (4), characterized in that: The outer wall of the fuel exchange circuit (4) is connected with a concentrator (2) on the left side, the left end of the concentrator (2) is connected with a pipeline I (1), the left end of the pipeline I (1) is connected with an electromagnetic valve (9), the left side of the electromagnetic valve (9) is connected with a check valve (10), the left side of the check valve (10) is connected with a pressure reducing valve (11), the left side of the pressure reducing valve (11) is connected with an air window (12) on the lower side, the left side of the pressure reducing valve (11) is connected with an atomizer (17) on the upper side, the left side of the atomizer (17) is connected with a high-pressure water tank (16), the left side of the pressure reducing valve (11) is connected with an oxygen tank (14) in the middle, the left side of the pressure reducing valve (11) is connected with carbon dioxide (15) in the middle, the left side of the pressure reducing valve (11) is connected with a gas reduction ground (13) in the middle, the right side of the fuel exchange circuit (4) is connected with a long plate (20).

2. The multi-layered microbial strain culture device according to claim 1, wherein: The outer wall of the fuel exchange circuit (4) is connected with a pipeline II (7) at the lower end, and the bottom end of the pipeline II (7) is connected with a receiver (8).

3. The multi-layered microbial seed culture device according to claim 1, wherein: The outer wall of the long plate (20) is connected with a virtual pipeline (6) at the bottom, and the right side of the long plate (20) and the virtual pipeline (6) is fixedly connected with a temperature and humidity instrument (5).

4. The multi-layered microbial seed culture device according to claim 1, wherein: The left side of the long plate (20) is connected with a hole (21), and the bottom of the long plate (20) is fixedly connected with an inclined plate (24).

5. The multi-tiered microbial seed culture device of claim 4, wherein: The bottom of the inclined plate (24) is fixedly connected with a drainage plate (23), and the top of the inclined plate (24) is provided with a magnetic stirrer (22).

6. The multi-tiered microbial seed culture device of claim 1, wherein: The right side of the long plate (20) is provided with a microprocessor (18), and the front side of the microprocessor (18) is fixedly connected with a controller (19).

7. The multi-tiered microbial seed culture device of claim 1, wherein: The outer wall of the fuel exchange circuit (4) is connected with a compressor (3) at the top.

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