Drosophila melanogaster non-diethyl ether anesthesia device

Abstract

The utility model relates to the technical field of fruit fly non-diethyl ether anesthesia, in particular to a fruit fly non-diethyl ether anesthesia device which comprises a box body, and a cover plate piece is installed at the upper end of the box body in a sealed mode. A heat preservation layer is arranged in the box body, a cavity is formed in the heat preservation layer, a filling sealing mechanism is arranged on the cavity and the box body in a penetrating mode, an inner waterproof layer is fixed to the inner side of the heat preservation layer, and a transparent storage box is placed in the inner waterproof layer. The self-made instrument equipment is novel and unique; the device can be recycled and widely popularized, and is superior to existing drosophila anesthesia equipment in all aspects; the sealing effect can be effectively guaranteed, the temperature of the environment where the transparent storage box is located can be effectively controlled to be constant, the temperature of the environment where the transparent storage box is located can be effectively reduced, and low-temperature drosophila anesthesia is achieved.

Description

Technical Field

[0001] This utility model relates to the field of non-ether anesthesia technology for fruit flies, and in particular to a non-ether anesthesia device for fruit flies. Background Technology

[0002] Drosophila is a commonly used model organism in biology teaching and research, playing an important role in fields such as genetics, development, aging, tumors, and neurodegenerative diseases. Drosophila-related experiments constitute a significant portion of university genetics lab teaching, serving crucial purposes for students to strengthen theoretical learning, practice experimental procedures, conduct scientific research, and innovate experimentally. Anesthetizing Drosophila is a critical step in experiments involving sex differentiation, morphological observation, hybridization, mutagenesis, and transfer. For a long time, domestic universities have used ether as an anesthesia agent for Drosophila due to its low cost and convenience. However, with the development of science and technology and the improvement of public awareness, the safety hazards of ether as an experimental reagent are increasingly attracting attention.

[0003] Ether, a Class II controlled precursor chemical in China, is volatile, flammable, and explosive. It also has a certain toxic effect on experimental operators, inhibiting the behavior of fruit flies for about 400 hours after they awaken, thus affecting experimental results. Therefore, exploring new safe and environmentally friendly methods of anestheticizing fruit flies has become an urgent need for biological experimental teaching.

[0004] Currently, the market price of fruit fly anesthesia equipment is high, and the costs of purchasing and subsequent maintenance are also high. Therefore, it is urgent to develop a self-made, cost-effective alternative to ether anesthesia equipment, which is of great value and significance to the teaching and research of genetics in biology and medical-related majors. Utility Model Content

[0005] The purpose of this invention is to address the shortcomings of existing technologies by proposing a non-ether anesthesia device for fruit flies.

[0006] In order to achieve the above purpose, the present invention adopts the following technical solutions:

[0007] A non-ether anesthesia device for fruit flies includes a housing, the upper end of which is sealed with a cover plate.

[0008] The box is equipped with an insulation layer, and a cavity is opened in the insulation layer. A filling and sealing mechanism is connected through the cavity and the box. An internal waterproof layer is fixed inside the insulation layer, and a transparent storage box is placed inside the internal waterproof layer.

[0009] An inner label is affixed to the transparent storage box, and an outer label is affixed to the outside of the box.

[0010] Compared with existing technologies, the self-made instrument and equipment in this application is novel and unique; it can be reused multiple times and widely promoted, and is superior to existing fruit fly anesthesia equipment in all aspects; it can effectively ensure the sealing effect and effectively control the constant temperature of the environment in which the transparent storage box is located; it adopts the principle of low temperature anesthesia, is non-toxic, harmless and pollution-free, does not require any experimental materials (such as ether), can be reused, and has low cost, making it suitable for group experiments by undergraduate students.

[0011] Preferably, the cover plate and the box body are hinged together, and rubber sealing strips are fixed at opposite ends of the cover plate and the box body. A hinge lock is installed at the non-hinged ends of the cover plate and the box body.

[0012] Furthermore, it facilitates the rotation of the cover plate relative to the housing, while the mutual contact between the cover plate and the housing effectively compresses the rubber sealing strip to ensure a sealing effect.

[0013] Preferably, the filling and sealing mechanism includes a connecting pipe that passes through one side of the lower end of the box body. One end of the connecting pipe passes through the box body and the insulation layer and extends into the cavity. A sealing plug is installed at the end of the connecting pipe located on the outside of the box body.

[0014] Furthermore, coolant can be injected into the cavity through the connecting pipe to reduce the temperature inside the chamber, and the internal waterproof layer ensures temperature stability, which helps to maintain a low temperature.

[0015] Preferably, the material of the housing and cover plate is either stainless steel or acrylic.

[0016] Furthermore, it helps extend the service life of components.

[0017] Preferably, the sealing plug is made of silicone material.

[0018] Furthermore, it can fully guarantee the sealing effect.

[0019] Preferably, the cavity is filled with a low-temperature non-condensable cryogenic liquid.

[0020] Furthermore, it can effectively reduce the temperature inside the chamber.

[0021] Preferably, a carrying handle is installed on the cover plate, and the exterior color of the box body, cover plate, carrying handle, and transparent storage box is the same.

[0022] Furthermore, in addition to color consistency, it can also quickly match by setting outer and inner labels to facilitate storage and placement.

[0023] The beneficial effects of the utility model are:

[0024] 1. The low-temperature non-condensable cryogenic liquid stored in the cavity can effectively reduce the temperature of the chamber and the internal waterproof layer, so as to effectively realize the non-ether anesthesia operation of fruit flies. Compared with the high volatility, toxicity and harmfulness of ether to humans, this application can better protect the test personnel.

[0025] 2. By using low-temperature anesthesia, the use of diethyl ether anesthesia, a Class II controlled substance in China, can be avoided, thus ensuring laboratory safety;

[0026] 3. The structure of this application is durable, can be reused multiple times and widely promoted. At present, there are fruit fly anesthesia devices available for sale, but they are expensive and cannot be purchased and used by undergraduate group experiments. This application has low production cost and is convenient for undergraduate group experiments. Attached Figure Description

[0027] Figure 1 This is a structural diagram of the present invention;

[0028] Figure 2 This is a structural diagram of the internal structure of the housing in this utility model;

[0029] Figure 3 This is a cross-sectional view of the box body in this utility model;

[0030] In the diagram: 1. Box body, 2. Rubber sealing strip, 3. Cover plate, 4. Handle, 5. Hinge lock, 6. Outer label, 7. Insulation layer, 8. Inner waterproof layer, 9. Transparent storage box, 10. Inner label, 11. Sealing plug, 12. Connecting pipe, 13. Cavity. Detailed Implementation

[0031] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0032] Reference Figure 1-3 A non-ether anesthesia device for fruit flies includes a box 1, with a cover plate 3 sealed at the upper end of the box 1. The materials of the box 1 and the cover plate 3 are either stainless steel or acrylic, which can extend the service life of the device. Moreover, in actual production and preparation, the appearance color can be selected as light and lively sky blue or warm and quiet pink, which is convenient for experimental personnel to use.

[0033] Reference Figure 1-3The chamber 1 has an insulation layer 7 inside, and a cavity 13 is opened in the insulation layer 7. The cavity 13 is filled with a low-temperature non-condensable refrigerant. The low-temperature non-condensable refrigerant can effectively reduce the temperature inside the chamber 1. At the same time, the low-temperature non-condensable refrigerant can be easily replaced as needed, and different types of low-temperature non-condensable refrigerants can be set to deal with different situations, thereby improving the practicality of the product. A filling and sealing mechanism is connected through the cavity 13 and the chamber 1. An internal waterproof layer 8 is fixed inside the insulation layer 7. A transparent storage box 9 is placed inside the internal waterproof layer 8. A handle 4 is installed on the cover plate 3. The exterior colors of the chamber 1, cover plate 3, handle 4 and transparent storage box 9 are consistent to ensure uniformity and facilitate use by laboratory personnel.

[0034] Reference Figure 1-2 An inner label 10 is affixed to the transparent storage box 9, and an outer label 6 is affixed to the outside of the box body 1. The inner label 10 and the outer label 6 are consistent, which makes it convenient for staff to use and place items accordingly.

[0035] Reference Figure 1-3 The cover plate 3 and the box body 1 are hinged together. Rubber sealing strips 2 are fixed at opposite ends of the cover plate 3 and the box body 1. Hinges 5 are installed at the non-hinged ends of the cover plate 3 and the box body 1. This can effectively ensure that the cover plate 3 and the box body 1 are connected and fixed. At the same time, the rubber sealing strips 2 can effectively seal the box body, prevent heat exchange with the outside, and ensure a constant temperature inside the box body 1.

[0036] Reference Figure 1-3 The filling and sealing mechanism includes a connecting pipe 12 that runs through one side of the lower end of the box 1. One end of the connecting pipe 12 passes through the box 1 and the insulation layer 7 and extends into the cavity 13. A sealing plug 11 is installed at the outer end of the connecting pipe 12. The sealing plug 11 is made of silicone material and can be effectively inserted and removed. The sealing plug 11 can effectively seal the connecting pipe 12 when inserted into it. Low-temperature non-condensable cryogenic liquid can be injected into the cavity 13 through the connecting pipe 12, which can reduce the temperature inside the box 1 and help to achieve low-temperature anesthesia of fruit flies. For many years, undergraduate students have used ether to anesthetize fruit flies by dripping it onto a cotton ball, placing the cotton ball in a sealed bottle, and then putting the fruit fly into the bottle. After a period of time, the fruit fly is anesthetized. This application also achieves low-temperature anesthesia by placing the fruit fly in a transparent storage box 9 and then waiting for a period of time. The operation process is similar and the anesthesia efficiency is also similar. At the same time, it can better protect the experimental personnel.

[0037] In this invention, a low-temperature non-condensable refrigerant can be injected into the cavity 13 through the connecting pipe 12, and the sealing operation can be effectively carried out through the sealing plug 11. This can effectively reduce the temperature inside the box 1, so as to perform low-temperature anesthesia on the fruit flies in the transparent storage box 9. Furthermore, the box 1, the rubber sealing strip 2, and the cover plate 3 can effectively seal the box and prevent heat exchange with the outside.

[0038] The above is only a preferred specific implementation method of the present invention, but the protection scope of the present invention is not limited to this. Any technician familiar with the technical field within the technical scope disclosed by the present invention can make equivalent replacements or changes based on the technical solution and utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A non-ether anesthesia device for fruit flies, comprising a housing (1), characterized in that: The upper end of the box (1) is sealed with a cover plate (3); The box (1) is provided with an insulation layer (7), and a cavity (13) is provided in the insulation layer (7). A filling and sealing mechanism is provided through the cavity (13) and the box (1). An internal waterproof layer (8) is fixed inside the insulation layer (7), and a transparent storage box (9) is placed inside the internal waterproof layer (8). An inner label (10) is affixed to the transparent storage box (9), and an outer label (6) is affixed to the outside of the box body (1).

2. The non-ether anesthesia device for fruit flies according to claim 1, characterized in that: The cover plate (3) and the box body (1) are hinged together. A rubber sealing strip (2) is fixed at one end of the cover plate (3) and the box body (1). A hinge lock (5) is installed at the unhinged end of the cover plate (3) and the box body (1).

3. The non-ether anesthesia device for fruit flies according to claim 1, characterized in that: The filling and sealing mechanism includes a connecting pipe (12) that runs through one side of the lower end of the box (1). One end of the connecting pipe (12) passes through the box (1) and the insulation layer (7) and extends into the cavity (13). A sealing plug (11) is installed at the end of the connecting pipe (12) located outside the box (1).

4. The non-ether anesthesia device for fruit flies according to claim 1, characterized in that: The materials for the box body (1) and the cover plate (3) are either stainless steel or acrylic.

5. A non-ether anesthesia device for fruit flies according to claim 3, characterized in that: The sealing plug (11) is made of silicone material.

6. The fruit fly non-ether anesthesia device according to claim 1, characterized in that: The cavity (13) is filled with a low-temperature non-condensable cryogenic liquid.

7. The non-ether anesthesia device for fruit flies according to claim 1, characterized in that: A carrying handle (4) is installed on the cover plate (3), and the exterior colors of the box body (1), cover plate (3), carrying handle (4) and transparent storage box (9) are the same.

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