Strain test tube culture medium split charging device

Abstract

The utility model discloses a strain test tube culture medium split charging device, which belongs to the technical field of mushroom mother strain cultivation and comprises a workbench, a rotating disc is rotatably connected onto the workbench, a plurality of sliders are uniformly arranged on the rotating disc along the circumference, and the sliders are slidably arranged on the rotating disc along the vertical direction. When the device is used, the test tube which is rotated to the right side on the rotating disc and is filled with a culture medium solution is firstly taken down by the manipulator and placed on the frame body, so that the test tube is obliquely stood, the culture medium solution in the test tube is solidified, and a culture medium mother strain inclined surface is formed in the test tube; then the manipulator clamps an empty test tube and inserts the test tube into the slide block on the right side; the rotating disc intermittently rotates once; at the moment, the driving device drives the sliding block located on the left side and the test tube to move upwards, the opening edge of the test tube is in butt joint with the subpackaging mechanism, then the culture medium solution in the subpackaging mechanism is poured into the test tube, subpackaging of one test tube is completed, and the purpose of automatically subpackaging the culture medium solution into the test tube is achieved.

Description

Technical Field

[0001] This utility model belongs to the field of mushroom mother culture technology, and in particular relates to a mushroom culture medium dispensing device. Background Technology

[0002] The process of mushroom breeding mainly includes three steps: mother culture, primary culture, and spawn culture. Mother culture is the first step in mushroom breeding, and it usually involves obtaining the spawn using tissue isolation or spore isolation methods. The mother culture medium is typically prepared using potato-agar medium, with a formula of 200g potato, 18-20g agar, and 20g glucose. The preparation process includes boiling the potatoes, filtering, adding agar and glucose, then dispensing the medium solution into test tubes and sterilizing them under high pressure. After inoculation, the test tubes are incubated at a constant temperature of around 25℃, and the mycelium will colonize the test tubes in about half a month. Well-cultured mother cultures can be stored for six months to one year.

[0003] After the culture medium solution is dispensed into test tubes, plugged with cotton plugs, and sterilized under high pressure, the test tubes are removed and tilted to stand. The culture medium solution inside the test tubes should not touch the cotton plugs. After a few hours, the culture medium solution inside the test tubes solidifies, forming a slant. At this point, the mother culture medium is ready. Currently, the culture medium solution is usually dispensed into test tubes manually, which is time-consuming and labor-intensive.

[0004] A search revealed patent number CN115478001B, which provides a device for dispensing microbial culture medium. The device includes a support and a rotating frame; multiple tube clamps, each holding a test tube containing solid culture medium; a vertical frame mounted on the support, with vertically oriented grooves; a lead screw connected to a bearing on the frame and connected to a first power device; a threaded sleeve threadedly connected to the lead screw, with its outer wall slidably connected to the grooves; and an annular hot air pipe connected to the threaded sleeve, connected to a hot air supply device, with multiple air jets on its inner wall, the inner wall of which can fit over the test tubes on the tube clamps. This device for dispensing microbial culture medium prevents contamination of the test tubes during dispensing, thereby improving the purity and activity of the colonies in the solid culture medium within the test tubes.

[0005] The above technical solution provides a method for inoculating the culture medium after it has been prepared, which facilitates high-temperature sterilization. However, the culture medium solution is usually dispensed into test tubes manually, which is time-consuming and labor-intensive. Utility Model Content

[0006] The purpose of this invention is to provide a microbial culture medium dispensing device for test tubes, which has the advantage of automatically dispensing culture medium solutions into test tubes, and solves the problem that the existing culture medium solution dispensing into test tubes is generally done manually, which is time-consuming and labor-intensive.

[0007] The present invention adopts the following technical solution: a bacterial culture medium dispensing device, including a workbench, a rotating disk rotatably connected to the workbench, a plurality of sliders evenly arranged along the circumference of the rotating disk, the sliders sliding on the rotating disk in the up and down direction, a test tube inserted into the insertion hole of each slider, a dispensing mechanism arranged on the left side of the workbench, and a driving device arranged on the left side of the upper surface of the workbench, the driving device being used to drive the sliders and test tubes rotated to the left side to move upward.

[0008] Furthermore, the dispensing mechanism includes a fixed pressure cabinet and a dispensing head. A pressure chamber is opened inside the dispensing head. The pressure cabinet is connected to the pressure chamber through a pressure pipe. A filling valve assembly is provided on the lower end face of the dispensing head. The input end of the filling valve assembly is connected to the pressure chamber. The output end of the filling valve assembly is located directly above the test tube on the left side. An opening device is also provided on the output end of the filling valve assembly.

[0009] Furthermore, the filling valve assembly includes a valve body, which is installed on the inner bottom wall of the pressure chamber of the dispensing head. The upper end face of the valve body is located inside the pressure chamber, and the lower end face of the valve body protrudes from the lower end face of the dispensing head. A valve cavity is formed on the upper end face of the valve body, and a valve hole is formed on the inner bottom wall of the valve cavity. A valve plate is slidably arranged inside the valve cavity. Several outwardly inclined guide grooves are formed on the upper end face of the valve plate. An elastic element is provided on the upper end face of the valve plate. The elastic element drives the valve plate to move downward continuously, so that the valve plate seals the valve hole of the valve cavity. A valve core is fixedly arranged on the lower end face of the valve plate. The actuating end of the opening device is connected to the lower end face of the valve plate.

[0010] Furthermore, the opening device includes a push rod fixedly disposed on the lower end face of the valve core, a plurality of connecting rods fixedly disposed on the lower part of the outer surface of the push rod, and an annular block fixedly disposed on the outside of the push rod, which is fixedly disposed on the outer end of each connecting rod.

[0011] Furthermore, a baffle is fixedly installed on the upper part of the inner wall of the valve cavity, and several channels are provided between the baffle and the valve body. The elastic element includes a check spring installed between the baffle and the inner bottom wall of the valve cavity, and the check spring drives the valve plate to move downward continuously.

[0012] Furthermore, limit plates are provided on the upper part of both sides of the slider, a return spring is fixedly provided on the lower end of the slider, a base plate is fixedly provided on the bottom end of the return spring, and two side plates are fixedly provided on the upper end of the base plate, with the top of each side plate fixedly provided to the lower end of the rotating disk.

[0013] Furthermore, the driving device includes a hydraulic telescopic rod fixedly mounted on the left side of the upper surface of the workbench. A pushing component is fixedly mounted on the output end of the hydraulic telescopic rod. The pushing component includes a push plate fixedly mounted on the output end of the hydraulic telescopic rod, and the push plate extends to the bottom of the test tube on the left side.

[0014] Furthermore, a vertical rod is fixedly provided on the upper end face of the push plate, a top rod is sleeved on the outer surface of the vertical rod, and a telescopic spring is fixedly provided between the bottom end of the top rod and the upper end face of the push plate, with the telescopic spring sleeved on the vertical rod.

[0015] Furthermore, a motor is fixedly installed on the lower end face of the workbench, and the output shaft of the motor is fixedly connected to the rotation shaft of the rotary disk.

[0016] Furthermore, a valve stem is coaxially fixedly mounted on the upper end face of the valve plate, and the valve stem is slidably mounted inside the baffle in the vertical direction, with a check spring sleeved on the valve stem.

[0017] Compared with the prior art, the beneficial effects of the present invention are:

[0018] I. This utility model, by setting up a dispensing mechanism, a rotating disk, and a driving device, allows the following operation: When the rotating disk rotates to the right side, the test tube already filled with culture medium solution is first removed by a robotic arm and placed on a frame, causing the test tube to tilt and stand still, allowing the culture medium solution inside to solidify and form a slanted surface of the culture medium mother stock. Then, the robotic arm picks up an empty test tube and inserts it into the slider on the right side. The rotating disk rotates intermittently once. At this time, the driving device drives the slider on the left side and the test tube to move upwards, so that the edge of the test tube aligns with the dispensing mechanism. Then, the culture medium solution in the dispensing mechanism is poured into the test tube, completing the dispensing of one test tube and achieving the purpose of automatically dispensing culture medium solution into test tubes.

[0019] II. This utility model, through the valve body, valve plate, push rod, annular block, and check spring, when in use, the driving device drives the slider on the left and the test tube to move upward, so that the edge of the test tube aligns with the annular block, and at the same time, it presses the annular block, causing the annular block to move upward, which in turn drives the push rod to move upward, so that the push rod pushes the valve plate to overcome the pressure of the check spring and move upward, so that the culture medium solution in the valve cavity flows into the test tube through the guide groove and valve hole of the valve plate, thus completing the dispensing of the test tube. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the overall three-dimensional structure of this utility model;

[0021] Figure 2 This is a front view structural diagram of the present utility model;

[0022] Figure 3 This is a three-dimensional structural diagram of the rotating disk in this utility model;

[0023] Figure 4 This is a schematic diagram of the internal three-dimensional structure of the rotating disk in this utility model;

[0024] Figure 5 This is a schematic diagram of the internal structure of the rotating disk in this utility model;

[0025] Figure 6 This is a three-dimensional structural diagram of the pressure cabinet in this utility model;

[0026] Figure 7 This is a schematic diagram of the internal three-dimensional structure of the dispensing head in this utility model;

[0027] Figure 8 This is a three-dimensional structural diagram of the pressure tube in this utility model;

[0028] Figure 9 This is a three-dimensional structural diagram of the baffle in this utility model;

[0029] Figure 10 This is a three-dimensional structural diagram of the connecting rod in this utility model;

[0030] Figure 11 This is a schematic diagram of the internal three-dimensional structure of the valve body in this utility model;

[0031] Figure 12 This is a three-dimensional structural diagram of the valve plate in this utility model;

[0032] Figure 13 This is a schematic diagram of the internal structure of the valve body in this utility model.

[0033] In the diagram, 1. Workbench; 2. Rotary disk; 3. Slider; 4. Test tube; 6. Pressure cabinet; 7. Dispensing head; 8. Pressure chamber; 9. Pressure pipe; 10. Filling valve assembly; 11. Valve body; 12. Valve cavity; 13. Valve hole; 14. Valve plate; 15. Guide groove; 16. Valve core; 17. Push rod; 18. Connecting rod; 19. Annular block; 20. Annular groove; 21. Annular notch; 22. Baffle; 23. Channel; 24. Check spring; 25. Valve stem; 26. Limiting plate; 27. Return spring; 28. Base plate; 29. ​​Side plate; 30. Hydraulic telescopic rod; 31. Push plate; 32. Vertical rod; 33. Top rod; 34. Telescopic spring; 35. Motor; 36. Rotating shaft. Detailed Implementation

[0034] Please see Figure 1-13 The present invention will now be described in detail with reference to the accompanying drawings and embodiments:

[0035] The microbial culture medium dispensing device of this utility model includes a workbench 1, a rotating disk 2 rotatably connected to the workbench 1, and a plurality of sliders 3 evenly arranged along the circumference of the rotating disk 2. The sliders 3 are slidably arranged on the rotating disk 2 in the vertical direction, and a test tube 4 is inserted into the insertion hole of each slider 3. A dispensing mechanism is arranged on the left side of the workbench 1, and a driving device is arranged on the left side of the upper end face of the workbench 1. The driving device is used to drive the sliders 3 and test tubes 4 rotated to the left to move upward, so that the rim of the test tube 4 aligns with the dispensing mechanism. Then, the culture medium solution in the dispensing mechanism is poured into the test tube 4, completing the dispensing of one test tube 4. In use, the right side of the workbench 1 is used for loading and unloading. The rotating disk 2 rotates intermittently, with the number of rotations in one revolution corresponding to the number of sliders 3. The test tube 4 on the right side of the rotating disk 2, which has been filled with culture medium solution, is first removed by the robotic arm and placed on a frame, so that the test tube 4 is tilted and left to stand. The culture medium solution in the test tube 4 solidifies, forming a slant of culture medium mother stock inside the test tube 4. Then the robotic arm picks up an empty test tube 4 and inserts it into the slider 3 on the right side. The rotating disk 2 rotates intermittently once. At this time, the driving device drives the slider 3 and test tube 4 on the left side to move upward, so that the rim of the test tube 4 aligns with the dispensing mechanism. Then the culture medium solution in the dispensing mechanism is poured into the test tube 4, completing the dispensing of one test tube 4.

[0036] In this embodiment, the dispensing mechanism includes a fixed pressure cabinet 6 and a dispensing head 7. A pressure chamber 8 is provided inside the dispensing head 7. The pressure cabinet 6 is connected to the pressure chamber 8 via a pressure pipe 9. A filling valve assembly 10 is provided on the lower end face of the dispensing head 7. The input end of the filling valve assembly 10 is connected to the pressure chamber 8, and the output end of the filling valve assembly 10 is located directly above the test tube 4 on the left side. An opening device is also provided at the output end of the filling valve assembly 10. During normal use, the culture medium solution in the pressure cabinet 6 flows into the pressure chamber 8 of the dispensing head 7 through the pressure pipe 9. When the rotating disk 2 rotates intermittently, the driving device drives the slider 3 and test tube 4 on the left side to move upwards, causing the edge of the test tube 4 to align with the output end of the filling valve assembly 10. Simultaneously, pressure is applied to the opening device, causing the filling valve assembly 10 to open, and the pressure of the dispensing head 7... The culture medium solution in the pressure chamber 8 flows in from the input end of the filling valve assembly 10 and flows out from the output end of the filling valve assembly 10, flowing into the test tube 4 to complete the dispensing of the test tube 4; the pressure cabinet 6 is existing technology, and the function of the pressure cabinet 6 is to provide culture medium solution at a set pressure; the pressure cabinet 6 usually includes supporting equipment, including a pressure pump, which is controlled by a pressure switch to start and stop. The pressure switch senses the pressure inside the pressure cabinet 6; when the pressure is lower than the set value, the pressure pump starts to deliver culture medium solution into the pressure cabinet 6, causing the pressure of the culture medium solution inside the pressure cabinet 6 to rise. When the culture medium solution rises to the set value, the pressure switch controls the pressure pump to stop. Usually, the upper part of the pressure cabinet 6 is filled with compressed air. If there is no compressed air, since the culture medium solution is incompressible, the pressure pump will start and stop too frequently.

[0037] In this embodiment, the filling valve assembly 10 includes a valve body 11, which is installed on the inner bottom wall of the pressure chamber 8 of the dispensing head 7. The upper end face of the valve body 11 is located inside the pressure chamber 8, and the lower end face of the valve body 11 protrudes from the lower end face of the dispensing head 7. A valve cavity 12 is formed on the upper end face of the valve body 11, and a valve hole 13 is formed on the inner bottom wall of the valve cavity 12. A valve plate 14 is slidably arranged inside the valve cavity 12. A plurality of outwardly inclined guide grooves 15 are formed on the upper end face of the valve plate 14 along the circumferential direction. An elastic element is provided on the upper end face of the valve plate 14. The elastic element drives... The moving valve plate 14 always moves downward, so that the valve plate 14 seals the valve hole 13 of the valve cavity 12; the actuating end of the opening device is connected to the lower end face of the valve plate 14; in use, the driving device drives the slider 3 on the left and the test tube 4 to move upward, so that the edge of the test tube 4 aligns with the opening device, and at the same time, it presses the opening device. The opening device moves upward and pushes the valve plate 14 to overcome the pressure of the elastic element and move upward, so that the culture medium solution in the valve cavity 12 flows into the test tube 4 through the guide groove 15 and the valve hole 13 of the valve plate 14, thus completing the dispensing of the test tube 4.

[0038] In this embodiment, a valve core 16 is fixedly provided on the lower end face of the valve plate 14. When the elastic element drives the valve plate 14 to move downward, so that the valve plate 14 seals the valve hole 13 of the valve cavity 12, the valve core 16 enters the valve hole 13 of the valve cavity 12, further achieving the sealing of the valve hole 13 and increasing the sealing performance of the valve hole 13; the execution end of the opening device is connected to the lower end face of the valve core 16.

[0039] In this embodiment, the opening device includes a push rod 17 fixedly disposed on the lower end face of the valve core 16. Several connecting rods 18 are fixedly disposed on the lower part of the outer surface of the push rod 17. An annular block 19 is disposed on the outside of the push rod 17, and each connecting rod 18 is fixedly disposed on the outer end of the annular block 19. The driving device drives the slider 3 on the left and the test tube 4 to move upward, so that the edge of the test tube 4 aligns with the annular block 19. At the same time, the annular block 19 is pressed, so that the annular block 19 moves upward and drives the push rod 17 to move upward through the connecting rod 18. The push rod 17 pushes the valve plate 14 to move upward against the pressure of the elastic element, so that the culture medium solution in the valve cavity 12 flows into the test tube 4 through the guide groove 15 and valve hole 13 of the valve plate 14, thus completing the dispensing of the test tube 4.

[0040] In this embodiment, the lower end face of the dispensing head 7 is provided with an annular groove 20 around the pressure chamber 8, and the annular block 19 is slidably disposed in the annular groove 20. When the test tube 4 moves upward and pushes the annular block 19 to move upward, the annular block 19 moves upward in the annular groove 20, which increases the stability of the annular block 19 moving up and down.

[0041] In this embodiment, an annular notch 21 is provided at the lower part of the outer surface of the annular block 19. When the driving device drives the slider 3 on the left and the test tube 4 to move upward, so that the edge of the test tube 4 aligns with the annular block 19, the edge of the test tube 4 enters the annular notch 21, thereby achieving the alignment of the edge of the test tube 4 with the annular notch 21 and the annular block 19.

[0042] In this embodiment, a baffle 22 is fixedly provided on the upper part of the inner wall of the valve cavity 12. A plurality of channels 23 are provided between the baffle 22 and the valve body 11. The elastic element includes a check spring 24 provided between the baffle 22 and the inner bottom wall of the valve cavity 12. The check spring 24 drives the valve plate 14 to move downwards at all times to seal the valve hole 13 of the valve cavity 12.

[0043] In this embodiment, a valve stem 25 is coaxially fixed on the upper end face of the valve plate 14. The valve stem 25 is slidably disposed in the baffle 22 in the vertical direction. A check spring 24 is sleeved on the valve stem 25. When the valve plate 14 moves up and down in the valve cavity 12 of the valve body 11, the valve plate 14 drives the valve stem 25 to move up and down in the baffle, which increases the stability of the up and down movement of the valve plate 14.

[0044] In this embodiment, limit plates 26 are protruding from the upper part of both sides of the slider 3. A return spring 27 is fixedly installed on the lower end face of the slider 3. A base plate 28 is fixedly installed at the bottom end of the return spring 27. Two side plates 29 are fixedly installed on the upper end face of the base plate 28. The top ends of the side plates 29 are fixedly installed with the lower end face of the rotating disk 2. When the return spring 27 is in a stretched state, the return spring 27 pulls the slider 3 to move downward, so that the limit plates 26 of the slider 3 contact the rotating disk 2. The limit plates 26 prevent the slider 3 from sliding further downward.

[0045] In this embodiment, the driving device includes a hydraulic telescopic rod 30 fixedly mounted on the left side of the upper surface of the workbench 1. A pushing assembly is fixedly mounted on the output end of the hydraulic telescopic rod 30. The pushing assembly includes a push plate 31 fixedly mounted on the output end of the hydraulic telescopic rod 30, and the push plate 31 extends to the lower part of the test tube 4 on the left side. When the hydraulic telescopic rod 30 extends upward, the push plate 31 pushes the test tube 4 upward, and the slider 3 follows the test tube 4 upward, so that the edge of the test tube 4 aligns with the annular block 19, and at the same time, it presses the annular block 19, causing the annular block 19 to move upward. The connecting rod 18 drives the push rod 17 to move upward, causing the push rod 17 to push the valve plate 14 to move upward against the pressure of the elastic element. This allows the culture medium solution in the valve cavity 12 to flow into the test tube 4 through the guide groove 15 and valve hole 13 of the valve plate 14, completing the dispensing of the test tube 4. After dispensing, the hydraulic telescopic rod 30 retracts downward, driving the push plate 31 to move downward. At this time, the return spring 27 pulls the slider 3 downward, which in turn causes the test tube 4 to move downward until the slider 3 is stopped by the limit plate 26. Then the rotating disk 2 rotates intermittently.

[0046] In the above scheme, since the test tube 4 is inserted into the insertion hole of the slider 3, pushing the test tube 4 upward will cause the slider 3 to move upward, resulting in a phenomenon where the slider 3 and the test tube 4 are not synchronized. To solve this problem, in this embodiment, a vertical rod 32 is fixedly provided on the upper end face of the push plate 31, and a top rod 33 is sleeved on the outer surface of the vertical rod 32. A telescopic spring 34 is fixedly provided between the bottom end of the top rod 33 and the upper end face of the push plate 31, and the telescopic spring 34 is sleeved on the vertical rod 32. When the output end of the hydraulic telescopic rod 30 extends upward and drives the push plate 31 to move upward, the top end of the top rod 33 is pre-positioned. Contacting the lower end of the slider 3, as the output end of the hydraulic telescopic rod 30 extends further, the push rod 33 slides downward on the vertical rod 32 to compress the telescopic spring 34. When the upper end of the push plate 31 contacts the bottom end of the test tube 4, the push plate 31 pushes the test tube 4 upward. The test tube 4 drives the slider 3 upward through the friction with the insertion hole of the slider 3. At the same time, the push rod 33 also pushes the slider 3 upward. The sum of the upward pushing force of the push rod 33 and the friction between the test tube 4 and the insertion hole of the slider 3 can overcome the tension of the return spring 27, so that the slider 3 and the test tube 4 move upward synchronously.

[0047] In this embodiment, a motor 35 is fixedly installed on the lower end face of the workbench 1. The output shaft of the motor 35 is fixedly installed with the rotation shaft 36 of the rotating disk 2. The rotation of the motor 35 drives the rotating disk 2 to rotate through the rotation shaft 36, thereby realizing the intermittent rotation of the rotating disk 2.

[0048] The working principle of this utility model is as follows: During use, the right side of the workbench 1 is the loading and unloading station. The rotating disk 2 rotates intermittently, with the number of rotations in one revolution corresponding to the number of sliders 3. Test tubes 4 on the right side of the rotating disk 2, already filled with culture medium solution, are first removed by the robotic arm and placed on a frame, causing the test tubes 4 to tilt and remain stationary. The culture medium solution inside the test tubes 4 solidifies, forming a sloping surface within the test tubes 4. Then, the robotic arm picks up an empty test tube 4 and inserts it into the slider 3 on the right side. The rotating disk 2 rotates intermittently once. At this time, the hydraulic telescopic rod 30 extends upward, pushing the test tube 4 upward through the push plate 31, and the slider 3 follows. The tube 4 moves upward, causing the rim of the test tube 4 to align with the annular block 19, while simultaneously pressing the annular block 19. This causes the annular block 19 to move upward, which in turn drives the push rod 17 upward via the connecting rod 18. The push rod 17 then pushes the valve plate 14 upward against the pressure of the elastic element, allowing the culture medium solution in the valve chamber 12 to flow into the test tube 4 through the guide groove 15 and valve hole 13 of the valve plate 14, thus completing the dispensing of the test tube 4. After dispensing, the hydraulic telescopic rod 30 retracts downward, causing the push plate 31 to move downward. At this time, the return spring 27 pulls the slider 3 downward, which in turn causes the test tube 4 to move downward. Then, the rotating disk 2 rotates intermittently.

Claims

1. A device for dispensing microbial culture medium in test tubes, comprising a workbench (1), characterized in that: A rotating disk (2) is rotatably connected to the workbench (1). Several sliders (3) are evenly arranged along the circumference of the rotating disk (2). The sliders (3) are slidably arranged on the rotating disk (2) in the up and down direction. A test tube (4) is inserted into the insertion hole of each slider (3). A dispensing mechanism is provided on the left side of the workbench (1). A driving device is provided on the left side of the upper end face of the workbench (1). The driving device is used to drive the sliders (3) and test tubes (4) that have rotated to the left side to move upward.

2. The microbial culture medium dispensing device according to claim 1, characterized in that: The dispensing mechanism includes a fixed pressure cabinet (6) and a dispensing head (7). A pressure chamber (8) is provided inside the dispensing head (7). The pressure cabinet (6) is connected to the pressure chamber (8) through a pressure pipe (9). A filling valve assembly (10) is provided on the lower end face of the dispensing head (7). The input end of the filling valve assembly (10) is connected to the pressure chamber (8). The output end of the filling valve assembly (10) is located directly above the test tube (4) on the left side. An opening device is also provided on the output end of the filling valve assembly (10).

3. The culture medium dispensing device for bacterial cultures in test tubes according to claim 2, characterized in that: The filling valve assembly (10) includes a valve body (11), which is installed on the inner bottom wall of the pressure chamber (8) of the dispensing head (7). The upper end face of the valve body (11) is located inside the pressure chamber (8), and the lower end face of the valve body (11) protrudes from the lower end face of the dispensing head (7). A valve cavity (12) is opened on the upper end face of the valve body (11), and a valve hole (13) is opened on the inner bottom wall of the valve cavity (12). A valve is slidably disposed inside the valve cavity (12). The valve plate (14) has several outwardly inclined guide grooves (15) on its upper end surface along the circumferential direction. An elastic element is provided on the upper end surface of the valve plate (14). The elastic element drives the valve plate (14) to move downward continuously, so that the valve plate (14) seals the valve hole (13) of the valve cavity (12). A valve core (16) is fixedly provided on the lower end surface of the valve plate (14). The actuating end of the opening device is connected to the lower end surface of the valve plate (14).

4. The culture medium dispensing device for bacterial cultures in test tubes according to claim 3, characterized in that: The opening device includes a push rod (17) fixedly disposed on the lower end face of the valve core (16), a plurality of connecting rods (18) fixedly disposed on the lower part of the outer surface of the push rod (17), and an annular block (19) fixedly disposed on the outside of the push rod (17) and fixedly disposed on the outer end of each connecting rod (18).

5. The culture medium dispensing device for bacterial cultures in test tubes according to claim 3, characterized in that: A baffle (22) is fixedly provided on the upper part of the inner wall of the valve cavity (12). Several channels (23) are provided between the baffle (22) and the valve body (11). The elastic element includes a check spring (24) provided between the baffle (22) and the inner bottom wall of the valve cavity (12). The check spring (24) drives the valve plate (14) to move downwards at all times.

6. The culture medium dispensing device for bacterial cultures in test tubes according to claim 1, characterized in that: Limiting plates (26) are provided on the upper part of both sides of the slider (3). A reset spring (27) is fixedly provided on the lower end face of the slider (3). A base plate (28) is fixedly provided on the bottom end of the reset spring (27). Two side plates (29) are fixedly provided on the upper end face of the base plate (28). The top of the side plates (29) is fixedly provided with the lower end face of the rotating disk (2).

7. The culture medium dispensing device for bacterial cultures in test tubes according to claim 6, characterized in that: The driving device includes a hydraulic telescopic rod (30) fixedly installed on the left side of the upper surface of the workbench (1). A pushing component is fixedly installed at the output end of the hydraulic telescopic rod (30). The pushing component includes a push plate (31) fixedly installed at the output end of the hydraulic telescopic rod (30). The push plate (31) extends to the bottom of the test tube (4) on the left side of the rotating disk (2).

8. The culture medium dispensing device for bacterial cultures in test tubes according to claim 7, characterized in that: A vertical rod (32) is fixedly provided on the upper end face of the push plate (31), and a top rod (33) is sleeved on the outer surface of the vertical rod (32). A telescopic spring (34) is fixedly provided between the bottom end of the top rod (33) and the upper end face of the push plate (31), and the telescopic spring (34) is sleeved on the vertical rod (32).

9. The culture medium dispensing device for bacterial cultures in test tubes according to claim 1, characterized in that: A motor (35) is fixedly installed on the lower end face of the workbench (1), and the output shaft of the motor (35) is fixedly installed with the rotation shaft (36) of the rotary disk (2).

10. The culture medium dispensing device for bacterial cultures in test tubes according to claim 5, characterized in that: The valve plate (14) is coaxially fixed with a valve stem (25) on its upper end face. The valve stem (25) is slidably disposed in the baffle (22) in the up and down direction. The check spring (24) is sleeved on the valve stem (25).

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