Fractional separation structure of continuous chromatography

Abstract

The utility model relates to the technical field of continuous chromatographic separation, and discloses a continuous chromatographic fractional separation structure which comprises a bottom plate and a separation structure, the separation structure is composed of a mobile phase tank body, a fluid conveying pipe, a conveying pump, a three-way pipe, a sample conveying pipe, a chromatographic column, a detector and a waste liquid pool, a switching mechanism for mounting and switching the chromatographic column is arranged at the top of the bottom plate, and a mounting mechanism for connecting the chromatographic column with a three-way pipeline and a detector is also arranged at the top of the bottom plate. According to the utility model, the chromatographic columns, the switching mechanism and the mounting mechanism are matched for use, so that the effect of grading and separating objects is achieved, meanwhile, a user can conveniently and quickly switch the chromatographic columns, different substances are conveniently separated, meanwhile, the switched chromatographic columns can be stably mounted, and the working efficiency is improved. And therefore, the practicability and the use convenience are further improved.

Description

Technical Field

[0001] This utility model relates to the field of continuous chromatography separation technology, specifically to a stepwise separation structure for continuous chromatography. Background Technology

[0002] Continuous chromatography is a highly efficient separation technique. The design of its fractional separation structure is crucial for the effective separation of different substances. The fractional separation principle of continuous chromatography is based on the difference in the partition coefficients of different substances between the stationary phase and the mobile phase. During the elution process using the mobile phase, different substances will exhibit different retention times, thereby achieving separation.

[0003] Most existing continuous chromatography fractionation structures use the same type of chromatographic column. This means that when different substances need to be separated, users need to disassemble the original chromatographic column and then reinstall a suitable column in the separation structure before separating the substances. This disassembly and reassembly of the column wastes a lot of time and affects the separation efficiency. Utility Model Content

[0004] The purpose of this invention is to provide a fractional separation structure for continuous chromatography, which solves the problem that most existing continuous chromatography fractional separation structures use the same type of chromatographic column. This means that when different substances need to be separated, the user needs to disassemble the original chromatographic column and then reinstall a suitable chromatographic column in the separation structure before separating the substances. This disassembly and reinstallation of the chromatographic column wastes a lot of time and affects the separation efficiency.

[0005] This utility model provides the following technical solution: a fractional separation structure for continuous chromatography, comprising a base plate and a separation structure. The separation structure consists of a mobile phase tank, a fluid delivery pipe, a delivery pump, a three-way connector, a sample delivery pipe, a chromatographic column, a detector, and a waste liquid tank. The mobile phase tank is connected to the delivery pump via the fluid delivery pipe. The end of the delivery pump away from the fluid delivery pipe is connected to the three-way connector. The two ends of the three-way connector away from the delivery pump are respectively connected to the sample delivery pipe and the chromatographic column. The chromatographic column is connected to a pipe on the detector. A pipe connected to the waste liquid tank is also provided on the side of the detector away from the chromatographic column. The chromatographic column is located on the top of the base plate. A switching mechanism for installing and switching the chromatographic column is provided on the top of the base plate. An installation mechanism for connecting the chromatographic column to the three-way connector and the detector is also provided on the top of the base plate.

[0006] As a preferred embodiment of the above technical solution, the switching mechanism includes a bearing seat mounted on the top of the base plate and an internal thread fixedly connected to the inside of the chromatographic column, as well as an annular plate fixedly connected to the inside of the chromatographic column. A support plate is fixedly connected to the inside of the bearing seat, and a rotating shaft is rotatably connected to the inside of the support plate. A turntable is fixedly connected to the top of the rotating shaft. The turntable has four threaded holes distributed at equal angles. The chromatographic column is threaded to the inside of the threaded holes. A bellows is threaded to the inside of the internal thread. A sealing ring is fixedly connected to the bottom of the bellows. A sealing groove is provided on the top of the annular plate.

[0007] As a preferred embodiment of the above technical solution, the bearing housing is mounted on the top of the base plate by several bolts. The support plate has a through hole, and a rotating bearing is installed inside the through hole. The rotating shaft is fixedly connected to the inside of the rotating bearing, and the rotating shaft is rotatably connected to the support plate through the rotating bearing. There are four chromatographic columns and four threaded holes. All four threaded holes are located on the turntable, and external threads are provided on the outer side of each of the four chromatographic columns. The four chromatographic columns are respectively connected to the inner side of the four threaded holes through the four external threads. The four chromatographic columns have the same specifications and dimensions.

[0008] As a preferred embodiment of the above technical solution, there are two internal threads, two bellows, two sealing rings, and two ring plates. The two internal threads are respectively located on the inner sides of both ends of the chromatographic column, and the two bellows are respectively threaded to the inner sides of the two internal threads. The two sealing rings are respectively fixedly connected to the bottom of the two bellows, and the two ring plates are respectively fixedly connected to the inner sides of both ends of the chromatographic column. Each ring plate has a sealing groove, and the two sealing rings are respectively inserted into the inner sides of the two sealing grooves. The size of the two sealing rings is adapted to the size of the two sealing grooves, and the diameter of each bellows is smaller than the diameter of the threaded hole.

[0009] With the above technical solution, users can quickly switch chromatographic columns using the switching mechanism.

[0010] As a preferred embodiment of the above technical solution, the installation mechanism includes a protrusion fixedly connected to the bellows and a shell plate fixedly connected to the top of the base plate. A sliding groove is inserted into the outside of the protrusion, and a bidirectional screw is threadedly connected to the outside of the sliding groove. A motor is fixedly connected to the top of the bidirectional screw.

[0011] As a preferred embodiment of the above technical solution, one end of the bidirectional screw is rotatably connected to the inner side of the shell plate via a rotating bearing, and the other end of the bidirectional screw passes through the shell plate and is fixedly connected to the motor. The motor is fixedly connected to the top of the shell plate. There are two slide grooves, both of which are threadedly connected to the bidirectional screw and slidably connected to the inner side of the shell plate. The number of protrusions is the same as that of the bellows, and each protrusion is fixedly connected to the outer side of each bellows. The size of each protrusion is adapted to the slide groove.

[0012] With the above technical solution, users can connect the chromatographic column and the three-way tube to the pipeline on the detector through the installation mechanism.

[0013] Compared with the prior art, the beneficial effects of this utility model are:

[0014] This invention achieves the effect of fractional separation of substances by using a combination of a mobile phase tank, fluid delivery pipe, delivery pump, three-way pipe, sample delivery pipe, chromatographic column, detector, waste liquid tank, base plate, switching mechanism, and installation mechanism. It also allows users to quickly switch chromatographic columns to facilitate the separation of different substances and provides stable installation of the switched chromatographic column, thereby further improving practicality and ease of use. Attached Figure Description

[0015] Figure 1 A first-view schematic diagram of a fractional separation structure in continuous chromatography;

[0016] Figure 2 This is a second-view schematic diagram of a fractional separation structure in continuous chromatography;

[0017] Figure 3 A side cross-sectional view of a fractional separation structure in continuous chromatography;

[0018] Figure 4 for Figure 2 Enlarged schematic diagram of the structure at point A in the middle;

[0019] Figure 5 for Figure 3 Enlarged schematic diagram of the structure at point B;

[0020] Figure 6 This is a schematic diagram of the overall process of the separation structure.

[0021] In the diagram: 1. Mobile phase tank; 2. Fluid delivery pipe; 3. Delivery pump; 4. Tee; 5. Sample delivery pipe; 6. Chromatographic column; 7. Detector; 8. Waste tank; 9. Base plate; 10. Switching mechanism; 11. Installation mechanism; 101. Bearing seat; 102. Support plate; 103. Rotating shaft; 104. Turntable; 105. Threaded hole; 106. Internal thread; 107. Ring plate; 108. Bellows; 109. Sealing ring; 1010. Sealing groove; 1101. Protrusion; 1102. Shell plate; 1103. Slide groove; 1104. Double-acting screw; 1105. Motor. Detailed Implementation

[0022] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention.

[0023] Example 1

[0024] like Figure 6 As shown, this utility model provides a technical solution: a fractional separation structure for continuous chromatography, including a base plate 9 and a separation structure. The separation structure consists of a mobile phase tank 1, a fluid delivery pipe 2, a delivery pump 3, a three-way pipe 4, a sample delivery pipe 5, a chromatographic column 6, a detector 7, and a waste liquid tank 8. The mobile phase tank 1 is connected to the delivery pump 3 via the fluid delivery pipe 2. The end of the delivery pump 3 away from the fluid delivery pipe 2 is connected to the three-way pipe 4. The two ends of the three-way pipe 4 away from the delivery pump 3 are respectively connected to the sample delivery pipe 5 and the chromatographic column 6. The chromatographic column 6 is connected to a pipe on the detector 7. A pipe connected to the waste liquid tank 8 is also provided on the side of the detector 7 away from the chromatographic column 6. The chromatographic column 6 is located on the top of the base plate 9, and the top of the base plate 9 is provided with a device for colorimetric analysis. The column 6 is installed and switched by a switching mechanism 10. The top of the base plate 9 is also equipped with an installation mechanism 11 for connecting the column 6 to the three-way tube 4 and the detector 7. This allows the user to use the delivery pump 3 to deliver the liquid in the mobile phase tank 1 to the three-way tube 4 through the fluid delivery tube 2. Then, the substance to be separated is delivered to the three-way tube 4 through the sample delivery tube 5. At this time, the fluid will carry the sample along with it. Then, the fluid will carry the sample into the column 6. The sample is then separated by the cooperation of the column 6 and the mobile phase. Each component enters the detector 7 under the influence of the mobile phase. The detector 7 will then detect the separated sample. After that, the mobile phase will carry the sample into the waste liquid pool 8 again, thus completing the fractionation and separation of substances.

[0025] like Figure 1 , Figure 2 , Figure 3 and Figure 5As shown, the switching mechanism 10 includes a bearing seat 101 mounted on the top of the base plate 9 and an internal thread 106 fixedly connected to the inside of the chromatographic column 6, as well as an annular plate 107 fixedly connected to the inside of the chromatographic column 6. A support plate 102 is fixedly connected to the inside of the bearing seat 101, and a rotating shaft 103 is rotatably connected to the inside of the support plate 102. A turntable 104 is fixedly connected to the top of the rotating shaft 103. The turntable 104 has four threaded holes 105 evenly distributed at angles. The chromatographic column 6 is threaded into the threaded holes 105. Inside the 05, the inner thread of the internal thread 106 is connected to a bellows 108. A sealing ring 109 is fixedly connected to the bottom of the bellows 108. A sealing groove 1010 is opened on the top of the ring plate 107. The bearing seat 101 is installed on the top of the base plate 9 by several bolts. A through hole is opened on the support plate 102. A rotating bearing is installed inside the through hole. The rotating shaft 103 is fixedly connected to the inside of the rotating bearing, and the rotating shaft 103 is rotatably connected to the support plate 102 through the rotating bearing. The chromatographic column 6 and the screw... Each column 6 has four pits 105, and all four threaded holes 105 are located on the turntable 104. External threads are provided on the outer sides of each of the four chromatographic columns 6. The four columns 6 are connected to the inner sides of the four threaded holes 105 via these external threads. The four columns 6 have identical dimensions. Each column has two internal threads 106, two bellows 108, two sealing rings 109, and two ring plates 107. Two internal threads 106 are located on the inner sides of both ends of each column 6, and two bellows 108 are threaded to... Two sealing rings 109 are fixedly connected to the bottom of two bellows 108, and two ring plates 107 are fixedly connected to the inner sides of both ends of the chromatographic column 6. Both ring plates 107 are provided with sealing grooves 1010. The two sealing rings 109 are inserted into the inner sides of the two sealing grooves 1010, and the size of the two sealing rings 109 is adapted to the two sealing grooves 1010. The diameter of each bellows 108 is smaller than the diameter of the threaded hole 105.

[0026] With the above technical solution, during use, the user can rotate the support plate 102 and move it to a position convenient for installing the chromatographic column 6. Then, multiple chromatographic columns 6 are installed into the multiple threaded holes 105 on the turntable 104. After that, the support plate 102 is rotated back to its original position, and then the turntable 104 is rotated until the protrusions 1101 on the outside of the two corrugated tubes 108 on one of the chromatographic columns 6 slide into the two sliding grooves 1103. Then, the two corrugated tubes 108 on the chromatographic column 6 and the three-way tube 4 are connected to the pipeline on the detector 7 through the installation mechanism 11. When it is necessary to switch the chromatographic column 6, the user can release the fixing of the chromatographic column 6 through the installation mechanism 11, and then rotate the turntable 104 again. Then, the protrusions 1101 on the outside of the chromatographic column 6 that meet the user's requirements are slid into the two sliding grooves 1103. Then, the chromatographic column 6 and the three-way tube 4 are connected to the pipeline on the detector 7 through the installation mechanism 11 again, thereby realizing the rapid switching of the chromatographic column 6, which facilitates the user to separate and detect different substances.

[0027] Example 2

[0028] like Figures 1-4As shown, this utility model provides a technical solution: a fractional separation structure for continuous chromatography, comprising a separation structure and a base plate 9 consisting of a mobile phase tank 1, a fluid delivery pipe 2, a delivery pump 3, a three-way pipe 4, a sample delivery pipe 5, a chromatographic column 6, a detector 7, and a waste liquid tank 8. The mobile phase tank 1 is connected to the delivery pump 3 via the fluid delivery pipe 2. The end of the delivery pump 3 away from the fluid delivery pipe 2 is connected to the three-way pipe 4. The two ends of the three-way pipe 4 away from the delivery pump 3 are respectively connected to the sample delivery pipe 5 and the chromatographic column 6. The chromatographic column 6 is connected to a pipe on the detector 7. A pipe connected to the waste liquid tank 8 is also provided on the side of the detector 7 away from the chromatographic column 6. The chromatographic column 6 is located on the top of the base plate 9. A switching mechanism 10 for installing and switching the chromatographic column 6 is provided on the top of the base plate 9. An installation mechanism 11 for connecting the chromatographic column 6 to the three-way pipe 4 and the detector 7 is also provided on the top of the base plate 9. The installation mechanism 11 includes a fixed connection to the corrugated... The tube 108 has a protrusion 1101 and a shell plate 1102 fixedly connected to the top of the base plate 9. A groove 1103 is inserted into the outside of the protrusion 1101, and a double-ended screw 1104 is threaded onto the outside of the groove 1103. A motor 1105 is fixedly connected to the top of the double-ended screw 1104. One end of the double-ended screw 1104 is rotatably connected to the inside of the shell plate 1102 through a rotating bearing, and the other end of the double-ended screw 1104 passes through the shell plate 1102 and is connected to the motor 1105. 5. Fixed connection: The motor 1105 is fixedly connected to the top of the shell plate 1102. There are two slide grooves 1103. Both slide grooves 1103 are threadedly connected to the bidirectional screw 1104, and both slide grooves 1103 are slidably connected to the inner side of the shell plate 1102. The number of protrusions 1101 is the same as that of the bellows 108. Each protrusion 1101 is fixedly connected to the outer side of each bellows 108, and the size of each protrusion 1101 is adapted to the slide groove 1103.

[0029] With the above technical solution, during use, the user can move the position of the chromatographic column 6 that meets the requirements through the switching mechanism 10. Then, the protrusion 1101 on the outside of the chromatographic column 6 that meets the user's requirements is slid into the two slide grooves 1103. Then, the motor 1105 is started. The motor 1105 drives the bidirectional screw 1104 to rotate. The rotation of the bidirectional screw 1104 drives the two slide grooves 1103 to move inside the shell plate 1102. The movement of the slide grooves 1103 drives the protrusion 1101 to move. The movement of the protrusion 1101 drives the bellows 108 to stretch. Then, as the slide grooves 1103 move, the two bellows 108 at both ends of the chromatographic column 6 will be inserted into the three-way pipe 4 and the pipe connected to the detector 7, respectively, thereby completing the installation of the chromatographic column 6. This not only makes it convenient for users to use, but also improves practicality.

[0030] The above embodiments are only used to illustrate the technical solution of this utility model, and are not intended to limit it.

Claims

1. A fractional separation structure for continuous chromatography, comprising a base plate (9) and a separation structure, the separation structure consisting of a mobile phase tank (1), a fluid delivery pipe (2), a delivery pump (3), a three-way connector (4), a sample delivery pipe (5), a chromatographic column (6), a detector (7), and a waste liquid tank (8), wherein the mobile phase tank (1) is connected to the delivery pump (3) via the fluid delivery pipe (2), one end of the delivery pump (3) away from the fluid delivery pipe (2) is connected to the three-way connector (4), the two ends of the three-way connector (4) away from the delivery pump (3) are respectively connected to the sample delivery pipe (5) and the chromatographic column (6), the chromatographic column (6) is connected to a pipe on the detector (7), and a pipe connected to the waste liquid tank (8) is also provided on the side of the detector (7) away from the chromatographic column (6), characterized in that: The chromatographic column (6) is set on the top of the base plate (9). The top of the base plate (9) is provided with a switching mechanism (10) for installing and switching the chromatographic column (6). The top of the base plate (9) is also provided with an installation mechanism (11) for connecting the chromatographic column (6) with the three-way tube (4) and the detector (7).

2. The fractional separation structure for continuous chromatography according to claim 1, characterized in that: The switching mechanism (10) includes a bearing seat (101) mounted on the top of the base plate (9) and an internal thread (106) fixedly connected to the inside of the chromatographic column (6), and an annular plate (107) fixedly connected to the inside of the chromatographic column (6). A support plate (102) is fixedly connected to the inside of the bearing seat (101), and a rotating shaft (103) is rotatably connected to the inside of the support plate (102). A turntable (104) is fixedly connected to the top of the rotating shaft (103). Four threaded holes (105) are provided on the turntable (104) at equal angles. The chromatographic column (6) is threaded to the inside of the threaded holes (105). A bellows (108) is threaded to the inside of the internal thread (106), and a sealing ring (109) is fixedly connected to the bottom of the bellows (108). A sealing groove (1010) is provided on the top of the annular plate (107).

3. The fractional separation structure for continuous chromatography according to claim 2, characterized in that: The bearing housing (101) is mounted on the top of the base plate (9) by several bolts. The support plate (102) has a through hole. A rotating bearing is installed inside the through hole. The rotating shaft (103) is fixedly connected to the inside of the rotating bearing. The rotating shaft (103) is rotatably connected to the support plate (102) through the rotating bearing. There are four chromatographic columns (6) and four threaded holes (105). The four threaded holes (105) are all opened on the turntable (104). The outer side of the four chromatographic columns (6) is provided with external threads. The four chromatographic columns (6) are respectively connected to the inner side of the four threaded holes (105) through the four external threads. The four chromatographic columns (6) have the same specifications and dimensions.

4. The fractional separation structure for continuous chromatography according to claim 2, characterized in that: There are two of each of the internal threads (106), bellows (108), sealing rings (109), and ring plates (107). The two internal threads (106) are respectively located on the inner sides of both ends of the chromatographic column (6), and the two bellows (108) are respectively threaded to the inner sides of the two internal threads (106). The two sealing rings (109) are respectively fixedly connected to the bottom of the two bellows (108). The two ring plates (107) are respectively fixedly connected to the inner sides of both ends of the chromatographic column (6), and both ring plates (107) are provided with sealing grooves (1010). The two sealing rings (109) are respectively inserted into the inner sides of the two sealing grooves (1010), and the size of the two sealing rings (109) is adapted to the two sealing grooves (1010). The diameter of each bellows (108) is smaller than the diameter of the threaded hole (105).

5. The fractional separation structure for continuous chromatography according to claim 2, characterized in that: The mounting mechanism (11) includes a protrusion (1101) fixedly connected to the bellows (108) and a shell plate (1102) fixedly connected to the top of the base plate (9). A sliding groove (1103) is inserted into the outside of the protrusion (1101), and a double-ended screw (1104) is threaded onto the outside of the sliding groove (1103). A motor (1105) is fixedly connected to the top of the double-ended screw (1104).

6. The fractional separation structure for continuous chromatography according to claim 5, characterized in that: One end of the bidirectional screw (1104) is rotatably connected to the inner side of the shell plate (1102) via a rotary bearing, and the other end of the bidirectional screw (1104) passes through the shell plate (1102) and is fixedly connected to the motor (1105). The motor (1105) is fixedly connected to the top of the shell plate (1102). There are two slide grooves (1103), both of which are threadedly connected to the bidirectional screw (1104) and slidably connected to the inner side of the shell plate (1102). The number of protrusions (1101) is the same as that of the bellows (108). Each protrusion (1101) is fixedly connected to the outer side of each bellows (108), and the size of each protrusion (1101) is adapted to the slide groove (1103).

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