Stem cell exosome extraction device

Abstract

The utility model relates to the technical field of biochemical experiment tools, in particular to a stem cell exosome extraction device which comprises a machine shell, a plurality of test tubes are arranged at the top of the machine shell, a driven part is arranged in the machine shell, the driven part is partially connected with the test tubes so as to drive the test tubes to rotate according to the axes of the test tubes, and a driving part is further arranged on the machine shell. The driving part is connected with the driven part so as to drive the driven part to rotate, and the conveying mechanism is used for dispersing stem cell liquid into the test tubes. According to the utility model, the centrifugal rotating structures of the driven part and the driving part are arranged, so that the test tube for accommodating stem cells centrifugally rotates according to the axis of the test tube, the test tube part is only shielded by the limiting structure, and most of the test tube is exposed, so that the test tube can be conveniently grasped and separated from the device; rotation of the test tubes and separation of the test tubes are not limited through the conveying mechanism, and stem cell liquid can be conveyed into the test tubes one by one when the conveying mechanism is connected with the test tubes.

Description

Technical Field

[0001] This utility model relates to the field of biochemical experimental equipment technology, and in particular to a stem cell exosome extraction device. Background Technology

[0002] Stem cell exosome extraction refers to the process of separating and extracting exosomes from stem cell culture medium or stem cell culture supernatant. Stem cell exosomes are mostly extracted by centrifugation.

[0003] Patent document CN117298984A discloses a mixing device for extracting stem cell exosomes, including a turntable shell and a rear cover. A turntable is rotatably mounted on top of the turntable shell and driven by a first stepper motor fixedly installed inside the turntable shell. The turntable engages with a test tube tray, which has a set of test tube placement chambers evenly distributed on it. A lifting seat is slidably mounted inside the rear cover, and a horizontally extending cantilever is provided on the lifting seat. A buffer solution dispensing head, an exosome dispensing head, and a contact liquid level sensor are fixedly mounted at the end of the cantilever. The ends of the buffer solution dispensing head, the exosome dispensing head, and the contact liquid level sensor probe are located on the same horizontal plane. The lifting seat is driven to move up and down by a second stepper motor. This invention can achieve automatic and precise gradient addition of buffer solution during stem cell exosome extraction.

[0004] When using the above-mentioned technology, the following technical problems were found in the existing technology: Test tubes need to be placed in a tray before centrifugation. However, after the separation device finishes working, the test tubes generally need to be removed from the tray. The upper part of the tray is partially exposed and adheres closely to the tray, making it difficult to handle and remove the centrifuge tubes after centrifugation. Furthermore, before centrifugation extraction, each test tube needs to be manually injected with an equal and appropriate amount of stem cell fluid. Therefore, a stem cell exosome extraction device is designed to provide an alternative technical solution to the above-mentioned technical problems. Utility Model Content

[0005] Therefore, it is necessary to provide a stem cell exosome extraction device to address the above-mentioned technical problems, in order to solve the technical problems of the inconvenience of detaching the test tube after centrifugation and the need to manually inject stem cell liquid into the test tube.

[0006] To solve the above-mentioned technical problems, the present invention adopts the following technical solution:

[0007] A stem cell exosome extraction device includes a casing and several test tubes containing stem cells, and further includes:

[0008] A driven part is provided on the housing, and a plurality of test tubes are connected to a portion of the driven part through a limiting structure. The plurality of test tubes rotate as the portion of the driven part rotates.

[0009] An active part, which is disposed on the housing and connected to the driven part, is used to drive the driven part to rotate;

[0010] The conveying mechanism is also located on the housing and includes several diverter tubes. The diverter tubes are connected to the portion of the conveying mechanism that extends out of the driven part on the housing. They are rotatably, telescopically, and detachably connected to the test tubes so that the diverter tubes do not rotate with the test tubes and can slide away from the test tubes.

[0011] In a preferred embodiment of the stem cell exosome extraction device provided by this utility model, a partition is integrally connected to the inner center of the housing, the driven part is located in the space above the partition inside the housing, and the active part is located inside the housing between the partition and the outer bottom of the housing.

[0012] In a preferred embodiment of the stem cell exosome extraction device provided by this utility model, the driven part includes a first gear, a second gear and a plurality of third gears. The first gear and the second gear are coaxially connected in the vertical direction and are rotatably connected to the housing and the partition, respectively. The plurality of third gears mesh circumferentially at equal intervals on the tooth edge of the outer circumference of the first gear. The bottom of the plurality of third gears is rotatably connected to the partition. The limiting structure is detachably connected between the housing and the test tube.

[0013] The limiting structure includes at least two convex strips circumferentially fixed to the outside of the test tube, and a limiting tube that can accommodate at least two of the convex strips and the test tube, wherein the limiting tube passes through the housing and is coaxially connected to the third gear.

[0014] In a preferred embodiment of the stem cell exosome extraction device provided by this utility model, the active part includes a reduction motor and a fourth gear. The reduction motor is inverted and installed at the bottom of the housing. The fourth gear is rotatably connected to the housing and the partition and is coaxially connected to the reduction motor. The fourth gear also meshes with the second gear.

[0015] As a preferred embodiment of the stem cell exosome extraction device provided by this utility model, the delivery mechanism further includes a container, a pump body, a delivery pipe, and a connector. The container and the pump body are both fixed to the bottom of the housing. The inlet of the pump body is connected to a pipe that extends into the interior of the container. The outlet of the pump body is connected to the delivery pipe. One end of the delivery pipe passes through the housing, the partition, the first gear, and the second gear, and protrudes to the top of the housing. The connector communicates with the end of the delivery pipe that protrudes to the top of the housing. The connector is also connected to several branch pipes.

[0016] As a preferred embodiment of the stem cell exosome extraction device provided by this utility model, each of the plurality of diversion tubes includes a first tube body, a second tube body and a plug. One end of the first tube body is rotatably and slidably connected to one end of the second tube body through a sealing element, and the other end of the first tube body is rotatably connected to the plug. The plug is snapped or threaded to the test tube, and the other end of the second tube body is connected to the connector.

[0017] The sealing element includes a rotary seal and a linear moving seal. The rotary seal is fixed on the first pipe body, and the linear moving seal is fixed outside the rotary seal.

[0018] As a preferred embodiment of the stem cell exosome extraction device provided by this utility model, it further includes multiple sets of support frames, which are respectively fixed on the machine housing in the area near the test tube, and a portion of the support frame is rotatably abutting against the outer surface of the test tube.

[0019] It is clear without a doubt that the technical solution described above in this application can solve the technical problem that this application aims to address.

[0020] At the same time, through the above technical solutions, this utility model has at least the following beneficial effects:

[0021] The present invention provides a stem cell exosome extraction device, which, through the centrifugal rotation structure of the driven part and the active part, allows the test tube containing stem cells to rotate centrifugally around its own axis, so that only part of the test tube is blocked by the limiting structure, while most of the test tube is exposed, thus making it easier to grasp the test tube and remove it from the device.

[0022] The present invention provides a stem cell exosome extraction device, which, through a delivery mechanism, can unrestrict the rotation or detachment of test tubes, and can deliver stem cell fluid one by one into the test tubes while in a connected state. Attached Figure Description

[0023] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0024] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0025] Figure 2 This is a schematic diagram of the structure of this utility model after the casing has been removed;

[0026] Figure 3This is a structural diagram showing how the driven part and the driving part of this utility model are connected when inverted;

[0027] Figure 4 This is a schematic diagram of the conveying mechanism of this utility model;

[0028] Figure 5 This is a schematic diagram showing how the test tube of this utility model is mounted on the driven part and connected to the conveying mechanism.

[0029] Figure 6 This is a partially cross-sectional view and further enlarged structural schematic diagram of the diversion tube of this utility model;

[0030] Figure 7 This is a schematic diagram of the limiting structure on the test tube and the third gear, as well as the structure of the support frame of this utility model.

[0031] In the diagram: 1. Housing; 2. Test tube; 3. Limiting structure; 31. Raised bar; 32. Limiting tube; 4. Conveying mechanism; 41. Diverter pipe; 411. First tube body; 412. Second tube body; 413. Plug; 42. Container; 43. Pump body; 44. Conveying pipe; 45. Connector; 5. First gear; 6. Second gear; 7. Third gear; 8. Gear motor; 9. Fourth gear; 10. Seal; 11. Support frame. Detailed Implementation

[0032] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present utility model and are not intended to limit the present utility model.

[0033] To enable those skilled in the art to better understand the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings.

[0034] It should be noted that, unless otherwise specified, the embodiments and features and technical solutions in the present invention can be combined with each other.

[0035] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0036] like Figure 1 and Figure 2As shown, this stem cell exosome extraction device includes a housing 1, with several test tubes 2 on the top of the housing 1. The test tubes 2 are used to hold a certain amount of stem cell liquid. The housing 1 is provided with a driven part, which is connected to several test tubes 2 to restrict the test tubes 2 to be located on the housing 1 and at the same time drive the test tubes 2 to rotate according to their own axis. This allows the test tubes 2 to rotate relative to the groove of the turntable and rotate due to the rotation of the turntable, allowing most of the test tubes 2 to leak out. This makes it easier to remove the test tubes 2 by force when centrifuging to extract and separate them.

[0037] An active part is also provided on the housing 1. The active part is connected to the driven part to drive the driven part to rotate. Furthermore, a partition is integrally connected to the inner middle part of the housing 1. The driven part is located in the space above the partition inside the housing 1. The active part is located in the space between the partition and the outer bottom of the housing 1.

[0038] It also includes a delivery mechanism 4. The suction part of the delivery mechanism 4 is located at the bottom of the housing 1, and the output part extends through the driven part and the center of the housing 1 to the top of the housing 1 and is connected to several test tubes 2 on the top of the housing 1. This allows a large amount of stem cell fluid to be dispersed in several test tubes 2, so that stem cells do not need to be manually added to several test tubes 2. Furthermore, the part of the delivery mechanism 4 connected to the test tubes 2 does not restrict the rotation of the test tubes 2 or the detachment of the test tubes 2, and can keep the test tubes 2 in a closed state when connected.

[0039] like Figure 3 The image shown is a view with the bottom facing upwards after being rotated 90 degrees. Figure 3 This perspective allows for a better demonstration of how the driving and driven parts are connected; the driven part includes a first gear 5, a second gear 6, and several third gears 7.

[0040] The first gear 5 and the second gear 6 are coaxially connected in the vertical direction and are rotatably connected to the housing 1 and the partition, respectively. It can be understood that when the second gear 6 rotates, the first gear 5 will also rotate. Several third gears 7 are circumferentially meshed on the toothed edges of the first gear 5. The bottoms of the several third gears 7 are rotatably connected to the partition. It can be understood that the rotation of the first gear 5 drives the several third gears 7 to rotate at their respective rotatable connection positions. Since the test tube 2 is connected to the third gear 7, the test tube 2 rotates along with the third gear 7.

[0041] The active part includes a geared motor 8 and a fourth gear 9. The geared motor 8 is inverted and installed at the bottom of the housing 1. The fourth gear 9 is rotatably connected to the housing 1 and the partition and coaxially connected to the geared motor 8. The fourth gear 9 also meshes with the second gear 6. The driving relationship is that the geared motor 8 drives the fourth gear 9 to rotate, the fourth gear 9 drives the second gear 6 to rotate, and finally the first gear 5 causes the test tube 2 located on the third gear 7 to rotate, so that centrifugation occurs in the test tube 2. As centrifugation continues, the exosomes are separated from the stem cells and located in the upper part of the test tube 2.

[0042] like Figure 4 As shown, the conveying mechanism 4 includes several diversion pipes 41 (only one diversion pipe 41 is shown in this figure for better illustration, but it can be determined from other figures that there are multiple diversion pipes 41), a container 42, a pump body 43, a conveying pipe 44, and a connector 45;

[0043] Both the container 42 and the pump body 43 are fixed to the bottom of the housing 1. The inlet of the pump body 43 is connected to a pipe, which extends into the interior of the container 42. The pump body 43 generates suction force when it works, and a large amount of stem cell fluid is drawn from the container 42 through the pipe. The container 42 is equipped with a connecting pipe, which is used to replenish the stem cell fluid in the container 42.

[0044] The output port of the pump body 43 is connected to the delivery pipe 44. One end of the delivery pipe 44 passes through the housing 1, the partition, the first gear 5 and the second gear 6 and protrudes to the top of the housing 1. The connector 45 is connected to the end of the delivery pipe 44 that protrudes to the top of the housing 1. The connector 45 is also connected to several diversion pipes 41. The pump body 43 delivers the aspirated stem cell fluid through the delivery pipe 44 to the connector 45. The connector 45 has multiple interfaces. The delivered stem cell fluid is divided into multiple streams through the multiple interfaces on the connector 45. It is then dispersed into several test tubes 2 through several diversion pipes 41. This avoids the need to manually add stem cell fluid to the test tubes 2 and quickly completes the addition of stem cells.

[0045] like Figure 5 As shown in the figure, the test tube 2 is mounted on the driven part and connected to the conveying mechanism 4. As shown in the figure, the test tube 2 is connected to the third gear 7 through the limiting structure 3, and the test tube 2 can also be detached from the third gear 7 through the limiting structure 3, that is, the test tube 2 can be removed from the third gear 7 so that the exosomes in the test tube 2 can be extracted after centrifugation.

[0046] The diverter tube 41 is rotatable, retractable, and detachable from the test tube 2, so that the test tube 2 can rotate with the third gear 7 without being connected to the diverter tube 41. At the same time, part of the diverter tube 41 can be retractable and detached from the test tube 2, so that the test tube 2 can be detached from the third gear 7 without being connected to the diverter tube 41.

[0047] Furthermore, such as Figure 6 As shown, several shunt tubes 41 (only one shunt tube 41 is shown in this figure, but the other shunt tubes 41 are similar) each include a first tube body 411, a second tube body 412, and a plug 413. One end of the first tube body 411 is rotatably and slidably connected to one end of the second tube body 412 through a sealing element 10, and the other end of the first tube body 411 is rotatably connected to the plug 413. The plug 413 is snap-fitted or threadedly connected to the test tube 2. The other end of the second tube body 412 is connected to the connector 45. Specifically, the first tube body 411 is vertical. One section of the first tube 411 overlaps with a section of the second tube 412 perpendicularly. One section of the first tube 411 extends into one section of the second tube 412. In this way, the first tube 411 can move up and down within one section of the second tube 412 through the sealing member 10, for example, moving upward to drive the cap 413 to disengage from the test tube 2. Then, the sealing member 10 drives the first tube 411 to rotate on the second tube 412 so that the cap 413 is not on the vertical line of the test tube 2, thereby allowing the test tube 2 to be vertically disengaged from the third gear 7.

[0048] Furthermore, the seal 10 is a component that can seal between two objects and allow the two objects to rotate relative to each other and slide and extend. Specifically, the seal 10 includes a rotary seal and a linear motion seal. More specifically, both the rotary seal and the linear motion seal belong to dynamic seals. The rotary seal is widely used to seal rods, shafts, pins, etc. that have rotational or oscillating motion to ensure the normal operation of the component and the sealing effect. The linear motion seal is used in various industrial equipment, such as hydraulic cylinders and pneumatic cylinders, which can effectively prevent liquid and gas leakage while maintaining the piston shaft to move. In this embodiment, the rotary seal and the linear motion seal are used in combination. That is, the rotary seal is fixed on the first tube 411 and the linear motion seal is fixed outside the rotary seal, so that the first tube 411 can rotate, extend, and slide on the second tube 412. The figure is only for illustration and the above description is the standard.

[0049] Furthermore, such as Figure 7 As shown, the limiting structure 3 includes at least two protrusions 31 (including but not limited to these, four are shown in the figure) that are circumferentially fixed to the outside of the test tube 2, and a limiting tube 32 that can accommodate at least two protrusions 31 and the test tube 2. The limiting tube 32 is coaxially connected to the third gear 7. By aligning the protrusions 31 on the test tube 2 with the slot of the limiting tube 32 and inserting them, a portion of the test tube 2 and its protrusions 31 can be placed inside the limiting tube 32, thereby connecting the test tube 2 with the third gear 7, so that the test tube 2 rotates with the rotation of the third gear 7.

[0050] Multiple sets of support frames 11 are fixed on the area near the test tube 2 on the casing 1. Part of the support frame 11 rolls against the outer surface of the test tube 2. Specifically, a rotating shaft is fixed on one side of the top of the support frame 11. The rotating shaft contacts the outer surface of the test tube 2 and provides further positioning of the test tube 2 based on the limiting structure 3, but does not restrict the rotation of the test tube 2, so that the test tube 2 can stably follow the rotation of the third gear 7. In addition, since the rotating shaft and the test tube 2 only have a line contact area, the rotating shaft will not generate excessive frictional resistance when the test tube 2 is removed. A little force is enough to make the test tube 2 detach from the contact with the rotating shaft.

[0051] The preferred embodiments of this utility model disclosed above are merely illustrative of the present utility model. These preferred embodiments do not exhaustively describe all details, nor do they limit the present utility model to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of the present utility model, thereby enabling those skilled in the art to better understand and utilize it. The present utility model is limited only by the claims and their full scope and equivalents.

Claims

1. A stem cell exosome extraction device, comprising a casing (1) and a plurality of test tubes (2) containing stem cells, characterized in that, Also includes: The driven part is provided on the housing (1), and a plurality of test tubes (2) are connected to a portion of the driven part through a limiting structure (3). The plurality of test tubes (2) rotate as the portion of the driven part rotates. An active part is provided on the housing (1) and connected to the driven part, and is used to drive the driven part to rotate; The conveying mechanism (4) is also provided on the housing (1) and includes a plurality of diversion tubes (41). The plurality of diversion tubes (41) are connected to the portion of the conveying mechanism (4) that extends out of the driven part on the housing (1). They are rotatably, extendably, and detachably connected to the plurality of test tubes (2) so that the diversion tubes (41) do not rotate with the rotation of the test tubes (2) and can slide away from the test tubes (2).

2. The stem cell exosome extraction device according to claim 1, characterized in that, A partition is integrally connected to the inner center of the housing (1). The driven part is located in the space above the partition inside the housing (1). The driving part is located in the space between the partition and the outer bottom of the housing (1).

3. The stem cell exosome extraction device according to claim 2, characterized in that, The driven part includes a first gear (5), a second gear (6) and several third gears (7). The first gear (5) and the second gear (6) are coaxially connected in the vertical direction and are rotatably connected to the housing (1) and the partition, respectively. Several third gears (7) mesh circumferentially at equal intervals on the tooth edge of the outer circumference of the first gear (5). The bottom of several third gears (7) is rotatably connected to the partition. The limiting structure (3) is detachably connected between the housing (1) and the test tube (2). The limiting structure (3) includes at least two protrusions (31) circumferentially fixed to the outside of the test tube (2), and a limiting tube (32) that can accommodate at least two of the protrusions (31) and the test tube (2). The limiting tube (32) passes through the housing (1) and is coaxially connected to the third gear (7).

4. The stem cell exosome extraction device according to claim 3, characterized in that, The active part includes a geared motor (8) and a fourth gear (9). The geared motor (8) is installed upside down at the bottom of the housing (1). The fourth gear (9) is rotatably connected to the housing (1) and the partition and is coaxially connected to the geared motor (8). The fourth gear (9) also meshes with the second gear (6).

5. The stem cell exosome extraction device according to claim 3, characterized in that, The conveying mechanism (4) also includes a container (42), a pump body (43), a conveying pipe (44), and a connector (45). The container (42) and the pump body (43) are both fixed to the bottom of the housing (1). The inlet of the pump body (43) is connected to a pipe, which extends into the interior of the container (42). The outlet of the pump body (43) is connected to the conveying pipe (44). One end of the conveying pipe (44) passes through the housing (1), the partition, the first gear (5), and the second gear (6) and protrudes to the top of the housing (1). The connector (45) is connected to the end of the conveying pipe (44) that protrudes to the top of the housing (1). The connector (45) is also connected to several branch pipes (41).

6. The stem cell exosome extraction device according to claim 5, characterized in that, Each of the aforementioned diversion tubes (41) includes a first tube body (411), a second tube body (412), and a plug (413). One end of the first tube body (411) is rotatably and slidably connected to one end of the second tube body (412) through a sealing element (10), and the other end of the first tube body (411) is rotatably connected to the plug (413). The plug (413) is snapped or threaded to the test tube (2), and the other end of the second tube body (412) is connected to the connector (45). The sealing element (10) includes a rotary seal and a linear moving seal. The rotary seal is fixed on the first tube body (411), and the linear moving seal is fixed outside the rotary seal.

7. The stem cell exosome extraction device according to claim 1, characterized in that, It also includes multiple sets of support frames (11), which are respectively fixed on the area of ​​the housing (1) near the test tube (2), and a portion of the support frame (11) is in rolling contact with the outer surface of the test tube (2).

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