Isothermal nucleic acid molecule fluorescence detector

Abstract

The utility model provides an isothermal nucleic acid molecule fluorescence detector and belongs to the technical field of nucleic acid detection. The detector comprises a detector body, a mounting groove is formed in the detector body, a pipe frame used for placing a sample single pipe or a sample connecting pipe is arranged in the mounting groove, an air inlet pipe and an air outlet pipe are arranged in the detector body, and a plurality of air inlets communicated with the air inlet pipe and a plurality of air outlets communicated with the air outlet pipe are formed in the inner wall of the mounting groove. A fan and a heating element are arranged in the air inlet pipe, and an oscillation assembly used for driving the pipe frame to move circumferentially in parallel is arranged in the mounting groove. When the fluorescence detector works, the fan and the heating element are started, hot air is blown into the mounting groove, the overall temperature in the mounting groove is increased, then the oscillation assembly drives the pipe frame to perform low-speed circumferential translation in the mounting groove, and all the sample connecting pipes on the pipe frame are subjected to small-amplitude circumferential shaking, so that reagents in the sample connecting pipes are continuously oscillated and mixed; the whole reagent is rapidly and uniformly heated, and the effect of improving the detection efficiency is achieved.

Description

Technical Field

[0001] This utility model relates to the field of nucleic acid detection technology, and in particular to an isothermal nucleic acid molecular fluorescence detector. Background Technology

[0002] An isothermal fluorescence detector is a high-precision instrument that integrates isothermal amplification and fluorescence signal detection. It operates based on fluorescence phenomena and can be used for nucleic acid detection. Isothermal amplification requires in vitro amplification of specific nucleic acid fragments at a constant temperature to obtain a large quantity of the target nucleic acid fragment.

[0003] Currently common isothermal fluorescence detectors work by designing a constant-temperature chamber, in which the sample to be tested is placed and gradually heated to the same temperature as the ambient temperature. However, during the detection process, the temperature rise inside and outside the isothermal amplification test tube of the pathogen sample nucleic acid is uneven, prolonging the detection time and causing inaccurate detection. Utility Model Content

[0004] In view of the above problems, this utility model provides an isothermal nucleic acid molecular fluorescence detector.

[0005] To achieve the above-mentioned objectives, the technical solution adopted by this utility model is as follows:

[0006] An isothermal nucleic acid molecular fluorescence detector is provided, including a detector body with a mounting groove. The mounting groove contains a tube rack for placing single sample tubes or sample tubes. The detector body contains an air inlet pipe and an air outlet pipe. The inner wall of the mounting groove has several air inlets communicating with the air inlet pipe and several air outlets communicating with the air outlet pipe. The air inlet pipe contains a fan and a heating element. The mounting groove contains an oscillation component for driving the tube rack to move in a parallel circumferential direction.

[0007] Furthermore, both the air inlet pipe and the air outlet pipe are arranged on the outer wall of the mounting groove along the circumference of the mounting groove, and multiple air inlets and outlets are evenly spaced along the circumference of the mounting groove, with the air inlet located below the air outlet.

[0008] Furthermore, air filters are installed in both the air inlet and outlet ducts.

[0009] Furthermore, the oscillation assembly includes a drive motor and at least two drive disks. The drive disks are rotatably mounted on the bottom wall of the mounting slot. A connecting seat is provided at the bottom of the tube rack. A rotating shaft is eccentrically mounted on the top wall of the drive disk. The top end of the rotating shaft is rotatably connected to the bottom wall of the drive disk. The drive motor is used to drive multiple drive disks to rotate synchronously in the same direction.

[0010] Furthermore, a drive gear is connected to the output end of the drive motor, and a gear ring is coaxially arranged on the outer wall of the drive disc. A toothed belt is meshed around the drive gear and the gear ring.

[0011] Furthermore, the tube rack includes multiple rows of positioning plates arranged in parallel, each of which has a number of insertion holes that are compatible with the sample connecting tubes.

[0012] Furthermore, the detector body is rotatably provided with a cover for opening and closing the mounting slot, the detector body is provided with an electromagnet for electromagnetically locking the cover, and a sealing ring is provided on the side of the cover that contacts the detector body.

[0013] The beneficial effects of this invention are as follows: After the sample tubes are installed in the tube rack on the main body of the detector, the cover is flipped to close the installation slot. The fan and heating element are started to blow hot air into the installation slot, increasing the overall temperature inside the installation slot. Then, the oscillation component drives the tube rack to slowly move in a circular motion in the installation slot, causing small circular oscillations to all the sample tubes on the tube rack. This causes the reagents in the sample tubes to continuously oscillate and mix, resulting in a uniform and rapid temperature rise inside and outside the reagents. This avoids the influence of edge effects on the detection and improves the detection efficiency. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the overall structure of the isothermal nucleic acid molecular fluorescence detector according to an embodiment of this application.

[0015] Figure 2 This is a schematic diagram of the internal structure of the isothermal nucleic acid molecular fluorescence detector according to an embodiment of this application.

[0016] Figure 3 This is a schematic diagram of the structure of the oscillation component according to an embodiment of this application.

[0017] The components include: 1. Instrument body; 11. Mounting slot; 2. Pipe rack; 21. Connecting seat; 22. Positioning plate; 23. Insertion hole; 3. Air inlet pipe; 31. Air inlet; 32. Fan; 33. Heating element; 4. Air outlet pipe; 41. Air outlet; 5. Vibration assembly; 51. Drive motor; 52. Drive disc; 53. Rotating shaft; 54. Drive gear; 55. Gear ring; 56. Gear belt; 6. Air filter; 7. Cover; 71. Electromagnet; 72. Sealing ring. Detailed Implementation

[0018] To better understand the above technical solutions, the following will provide a detailed explanation of the technical solutions in conjunction with the accompanying drawings and specific implementation methods.

[0019] This application discloses an isothermal nucleic acid molecular fluorescence detector, referring to... Figure 1 , Figure 2 and Figure 3The system includes a detector body 1, which has a mounting groove 11 containing a tube rack 2 for holding single sample tubes or sample tubes (hereinafter referred to as sample tubes). Several air inlets 31 and several air outlets 41 are located on the inner wall of the mounting groove 11. The detector body 1 contains an air inlet pipe 3 and an air outlet pipe 4. One end of the air inlet pipe 3 connects to the air inlet 31, and the other end faces the outside of the detector body 1. One end of the air outlet pipe 4 connects to the air outlet 41, and the other end faces the outside of the detector body 1. A fan 32 and a heating element 33 are located inside the air inlet pipe 3. When the detector body 1 is operating, the sample tubes are mounted on the tube rack 2. The fan 32 is activated, blowing air into the mounting groove 11, and the heating element 33 heats the air, thereby raising the temperature inside the mounting groove 11. This overall heating of the environment inside the mounting groove 11 ensures uniform heating of the sample tubes within it.

[0020] Specifically, the heating element 33 is a heating wire, and both the air inlet pipe 3 and the air outlet pipe 4 have annular portions, which are installed circumferentially on the outer wall of the mounting groove 11. Multiple air inlets 31 and 41 are evenly spaced along the circumference of the mounting groove 11, with the air inlet 31 located below the air outlet 41. During the heating and blowing process into the mounting groove 11, the air flows upwards within the mounting groove 11, and the circumferentially arranged air inlets 31 ensure that the sample connecting tubes on the tube rack 2 are fully heated. The upward airflow also prevents impurities from entering through the opening at the top of the sample connecting tubes and contaminating the sample.

[0021] Furthermore, air filters 6 are installed in both the air inlet pipe 3 and the air outlet pipe 4. The air filters 6 can filter the air that comes into contact with the mounting slot 11, so as to avoid the contamination of the mounting slot 11 by miscellaneous bacteria.

[0022] In this embodiment, the oscillation assembly 5 includes a drive motor 51 and at least two drive disks 52. The drive disks 52 are rotatably mounted on the bottom wall of the mounting groove 11. A connecting seat 21 is provided at the bottom of the tube rack 2. A rotating shaft 53 is eccentrically mounted on the top wall of the drive disks 52. The top end of the rotating shaft 53 is rotatably connected to the bottom wall of the drive disks 52. The drive motor 51 is used to drive the multiple drive disks 52 to rotate synchronously in the same direction. When the drive motor 51 starts, the multiple drive disks 52 rotate synchronously in the same direction, and through the rotating shaft 53 on the drive disks 52, drive the connecting seat 21 to move horizontally in a circular parallel motion, thereby shaking the sample tubes placed on the tube rack 2. This causes the sample inside and outside the sample tubes to continuously oscillate and mix, and ensures that during the overall heating of the mounting groove 11 and the sample tubes, the reagents inside and outside the sample tubes are heated uniformly, avoiding the influence of edge effects on detection and improving isothermal amplification efficiency. Specifically, the output end of the drive motor 51 is connected to the drive gear 54, and a gear ring 55 is coaxially arranged on the outer wall of the drive disk 52. A toothed belt 56 is meshed around the drive gear 54 and the gear ring 55.

[0023] The detector body 1 is equipped with a control system, which is electrically connected to actuators such as the fan 32, heating element 33, and drive motor 51, and controls the operation of each actuator. A temperature sensor is installed in the mounting slot 11 to detect the temperature of the mounting slot 11 in real time. The control system controls the fan 32 and heating wire to operate, raising the temperature in the mounting slot 11. When the temperature in the mounting slot 11 reaches the set temperature, the control system controls the fan 32 and heating wire to operate intermittently to maintain a constant temperature in the mounting slot 11 until the nucleic acid molecule fluorescence detection operation is completed.

[0024] To maintain the stability of the sample tubes during oscillation on the tube rack 2, in this embodiment, the tube rack 2 includes multiple rows of parallel and spaced positioning plates 22. Each positioning plate 22 has several insertion holes 23 adapted to the sample tubes. The positioning plates 22 are distributed vertically at intervals. Connecting rods are vertically fixed at the four corners of the connecting seat 21, and the connecting rods are fixedly connected to the four corners of the positioning plates 22. When inserting the sample tubes onto the tube rack 2, the sample tubes simultaneously pass through the insertion holes 23 on each positioning plate 22. The constraint effect of the positioning plates 22 on the sample tubes ensures that the sample tubes remain vertically inserted during oscillation, which is beneficial for accurate alignment of the sample tubes during subsequent fluorescence detection.

[0025] In this embodiment, a cover 7 is rotatably mounted on the top of the detector body 1. The cover 7 is used to open and close the mounting groove 11. An electromagnet 71 is provided on the detector body 1. The electromagnet 71 is used to magnetically attract and fix the movable end of the cover 7. A sealing ring 72 is provided on the side of the cover 7 that contacts the detector body 1. When the cover 7 is closed on the mounting groove 11 and magnetically attracted and fixed by the electromagnet 71, the gap between the cover 7 and the mounting groove 11 is completely sealed by the sealing ring 72, further eliminating external interference during the sample tube detection process, and allowing the environment inside the mounting groove 11 to heat up rapidly when heated.

[0026] Those skilled in the art will understand that although preferred embodiments of the present invention have been described, those skilled in the art, once they understand the basic inventive concept, can make other changes and modifications to these embodiments. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments as well as all changes and modifications falling within the scope of the present invention. Clearly, those skilled in the art can make various alterations and modifications to the present invention without departing from its spirit and scope. Thus, if these modifications and modifications of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention also intends to include these modifications and modifications.

Claims

1. An isothermal nucleic acid molecular fluorescence detector, characterized in that: The instrument includes a detector body (1), on which a mounting groove (11) is provided. A tube rack (2) for placing a single sample tube or a sample tube is provided in the mounting groove (11). An air inlet pipe (3) and an air outlet pipe (4) are provided in the detector body (1). Several air inlets (31) communicating with the air inlet pipe (3) and several air outlets (41) communicating with the air outlet pipe (4) are provided on the inner wall of the mounting groove (11). A fan (32) and a heating element (33) are provided in the air inlet pipe (3). An oscillation component (5) for driving the tube rack (2) to move in parallel circumference is provided in the mounting groove (11).

2. The isothermal nucleic acid molecular fluorescence detector according to claim 1, characterized in that, The air inlet pipe (3) and the air outlet pipe (4) are both arranged on the outer wall of the mounting groove (11) along the circumference of the mounting groove (11). The air inlet (31) and the air outlet (41) are both evenly spaced along the circumference of the mounting groove (11). The air inlet (31) is located below the air outlet (41).

3. The isothermal nucleic acid molecular fluorescence detector according to claim 2, characterized in that, Air filters (6) are installed in both the air inlet pipe (3) and the air outlet pipe (4).

4. The isothermal nucleic acid molecular fluorescence detector according to claim 1, characterized in that, The oscillation assembly (5) includes a drive motor (51) and at least two drive disks (52). The drive disks (52) are rotatably mounted on the bottom wall of the mounting groove (11). A connecting seat (21) is provided at the bottom of the tube frame (2). A rotating shaft (53) is eccentrically mounted on the top wall of the drive disks (52). The top end of the rotating shaft (53) is rotatably connected to the bottom wall of the drive disks (52). The drive motor (51) is used to drive multiple drive disks (52) to rotate synchronously in the same direction.

5. The isothermal nucleic acid molecular fluorescence detector according to claim 4, characterized in that, The output end of the drive motor (51) is connected to a drive gear (54), and a gear ring (55) is coaxially arranged on the outer wall of the drive disk (52). A toothed belt (56) is meshed around the drive gear (54) and the gear ring (55).

6. The isothermal nucleic acid molecular fluorescence detector according to claim 4, characterized in that, The tube rack (2) includes multiple rows of positioning plates (22) arranged in parallel, and each of the multiple rows of positioning plates (22) has a number of insertion holes (23) that are adapted to the sample connecting tube.

7. The isothermal nucleic acid molecular fluorescence detector according to claim 1, characterized in that, The detector body (1) is rotatably provided with a cover (7) for opening and closing the mounting groove (11), and the detector body (1) is provided with an electromagnet (71) for electromagnetically locking the cover (7). A sealing ring (72) is provided on the side of the cover (7) that contacts the detector body (1).

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