Cell flow suspension culture device capable of simulating blood flow

Abstract

The utility model discloses a cell flow suspension culture device capable of simulating blood flow, which comprises a culture dish filled with a culture medium, and the lower part of the culture dish is connected with a peristaltic pump through a hard conduit; one end of the hard conduit is connected with the bottom of the culture dish, and the other end of the hard conduit is connected with the soft conduit in the peristaltic pump; and a driving motor for controlling the culture medium in the culture dish, the hard guide pipe and the soft guide pipe to circularly flow in a reciprocating manner is mounted on the peristaltic pump. According to the cell flow suspension culture device for simulating blood flow, the principle that cardiac pacing is used as power and the flow velocity of blood vessels is uniformly controlled is combined, the power of the peristaltic pump is used for simulating a cardiac pacing power source, and uniform flow velocity is provided for a culture medium in the culture dish, so that liquid splashing is avoided, and the cell flow suspension culture device is convenient to use. And the method is closer to cell flow suspension culture of blood flow, and can be used for biological characteristic research of target cell fluid dynamics.

Description

Technical Field

[0001] This invention relates to the technical field of cell culture, and in particular to a cell flow suspension culture device that simulates blood flow, mainly used to study the growth status and biological characteristics of target cells in a non-adherent flow environment. Background Technology

[0002] Cell adherent culture and simple vibrating suspension culture are relatively easy to perform, but there is no ideal device that can simulate blood flow, control the flow frequency and speed, and keep cells in suspension without adhering to the wall.

[0003] Currently, cell suspension culture typically involves loading cells into a cell culture dish containing culture medium, then fixing the dish on a horizontal shaker. The shaker is then rotated at a constant speed, causing the cells to move within the culture medium and preventing sedimentation. However, this method has two drawbacks:

[0004] 1. Since petri dishes are generally open containers, vigorous shaking can cause the culture medium to splash, affecting the experimental results;

[0005] 2. Uneven shaking of the horizontal shaker leads to uneven flow rate of the culture medium in the petri dish. The flow rate is fast in some places and slow in others, which fails to achieve the effect of uniform flow rate and unified environment, and will also affect the experimental results, which are significantly different from the blood flow environment. Utility Model Content

[0006] In order to overcome the shortcomings of the prior art, the technical problem to be solved by this utility model is to provide a cell flow suspension culture device that simulates blood flow. It has a simple structure and can solve the problems of liquid splashing and unstable culture medium flow rate during suspension culture.

[0007] To achieve the aforementioned objective, this utility model provides a cell flow suspension culture device that simulates blood flow, comprising a culture dish containing culture medium, wherein the lower part of the culture dish is connected to a peristaltic pump via a rigid conduit; one end of the rigid conduit is connected to the bottom of the culture dish, and the other end of the rigid conduit is connected to a flexible conduit inside the peristaltic pump; the peristaltic pump is equipped with a drive motor for controlling the reciprocating flow of the culture medium in the culture dish, the rigid conduit, and the flexible conduit.

[0008] Furthermore, the peristaltic pump is equipped with rollers that are driven by the drive motor to rotate in both the forward and reverse directions. When the rollers rotate, they squeeze the soft tubing, so that the culture medium in the culture dish, hard tubing, and soft tubing reaches a speed similar to that of blood flow.

[0009] Preferably, the rigid conduit is provided with a filter membrane to prevent cells in the culture medium from flowing into the flexible conduit.

[0010] Furthermore, the rigid conduit includes a first rigid conduit and a second rigid conduit, one end of which is connected to the bottom sides of the culture dish, and the other end of which is connected to the two ends of the soft conduit, respectively.

[0011] Preferably, the first rigid conduit is provided with a first filter membrane, and the second rigid conduit is provided with a second filter membrane.

[0012] Preferably, the flexible conduit is U-shaped, with both ends of its U-shaped opening extending out of the peristaltic pump housing and connected to the first rigid conduit and the second rigid conduit.

[0013] Compared with the prior art, the cell flow suspension culture device simulating blood flow of this invention has at least the following beneficial effects and advantages:

[0014] 1. The cell flow suspension culture device that simulates blood flow of this utility model combines the principle of uniform control of blood flow velocity by using cardiac pacing as the power source. It uses the power of a peristaltic pump to simulate the power source of cardiac pacing and provides a uniform flow velocity to the culture medium in the culture dish. This achieves the effect of not causing liquid splashing and stabilizing the flow velocity and frequency of the culture medium. It is a cell flow suspension culture that is closer to blood flow and can be used for the study of biological characteristics of target cell fluid dynamics.

[0015] 2. This device uses a peristaltic pump as its power source, providing the culture medium with flow rates in both forward and reverse directions. The peristaltic pump is connected to the culture dish by a conduit, and a filter membrane is connected between the conduit and the peristaltic pump. The filter membrane prevents cells from flowing into the pump, reducing damage to the cells. This device establishes a microenvironment similar to the function of the heart and blood vessels. By using the power of the pump, the culture medium in the conduit and culture dish is driven to flow at a rate similar to that of blood in blood vessels. This solves the problems of liquid splashing and uneven flow rate of the culture medium, and can simultaneously simulate the microenvironment of human blood flow rate, achieving true cell suspension culture. This invention is a cell flow suspension culture device that simulates the flow rate of the heart's blood supply microenvironment, and can be used to study the growth status and biological characteristics of target cells in a non-adherent flow environment. Attached Figure Description

[0016] To more clearly illustrate the technical solution of this utility model, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0017] Figure 1 This is a schematic diagram of the structure of the cell flow suspension culture device for simulating blood flow according to this utility model;

[0018] Figure 2 This is a cross-sectional view of the cell flow suspension culture device for simulating blood flow according to this invention.

[0019] In the diagram: 10-culture medium, 20-culture dish, 30-rigid tubing, 31-first rigid tubing, 32-second rigid tubing, 40-filter membrane, 41-first filter membrane, 42-second filter membrane, 50-soft tubing, 60-peristaltic pump, 70-roller. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0021] See below. Figures 1 to 2 This invention provides a detailed description of the cell flow suspension culture device for simulating blood flow.

[0022] The cell flow suspension culture device for simulating blood flow provided by this utility model mainly includes a culture dish 20, a rigid conduit 30, a filter membrane 40, and a peristaltic pump 60. The culture dish 20 contains culture medium 10. The bottom of the culture dish 20 is connected to one end of the rigid conduit 30, and the other end of the rigid conduit 30 is connected to the soft conduit 50 inside the peristaltic pump 60.

[0023] The peristaltic pump 60 is equipped with a drive motor (not shown in the figure) for controlling the reciprocating flow of culture medium 10 in the culture dish 20, rigid tubing 30, and flexible tubing 50. The peristaltic pump 60 contains rollers 70 that are driven by the drive motor to rotate in both forward and reverse directions. When the rollers 70 rotate, they compress the flexible tubing 50, causing the culture medium 10 in the culture dish 20, rigid tubing 30, and flexible tubing 50 to reach a speed similar to that of blood flow.

[0024] In addition, the rigid tubing 30 is provided with a filter membrane 40 to prevent cells in the culture medium 10 from flowing into the flexible tubing 50. Specifically, the rigid tubing 30 includes a first rigid tubing 31 and a second rigid tubing 32. One end of the first rigid tubing 31 and the second rigid tubing 32 are respectively connected to the bottom sides of the culture dish 20, and the other ends of the first rigid tubing 31 and the second rigid tubing 32 are respectively connected to the two ends of the flexible tubing 50. The first rigid tubing 31 is provided with a first filter membrane 41, and the second rigid tubing 32 is provided with a second filter membrane 42.

[0025] In this invention, the flexible conduit 50 is U-shaped, with both ends of its U-shaped opening extending out of the housing of the peristaltic pump 60 and connected to the first rigid conduit 31 and the second rigid conduit 32. The roller 70 contacts the conduit at the bottom of the flexible conduit 50. When the roller 70 rotates, it squeezes the flexible conduit 50, causing the culture medium 10 in the circulation loop composed of the culture dish 20, the first rigid conduit 31, the first filter membrane 41, the flexible conduit 50, the second filter membrane 42, and the second rigid conduit 32 to circulate repeatedly.

[0026] Below, refer to Figure 1 and Figure 2 Based on the above description of structural features, the working principle of the cell flow suspension culture device simulating blood flow of this utility model is described as follows:

[0027] When the device starts operating, the drive motor first rotates in the forward direction, which in turn drives the roller 70 inside the peristaltic pump 60 to rotate in the forward direction, squeezing the flexible tubing 50. This causes the culture medium 10 in the culture dish 20, rigid tubing 30, and flexible tubing 50 to reach a speed close to that of blood flow. At this time, when the cells in the culture medium 10 reach the filter membrane on the right (first filter membrane 41), the first filter membrane 41 traps the cells, preventing them from flowing into the flexible tubing 50 inside the peristaltic pump 60. Conversely, the drive motor rotates in the reverse direction, causing the culture medium 10 to flow in the opposite direction at a uniform speed, carrying... Cells trapped by the right-hand filter membrane (first filter membrane 41) move in the opposite direction to the left-hand filter membrane (second filter membrane 42). Upon reaching their destination, they are trapped by the second filter membrane 42, preventing them from entering the soft catheter 50 of the peristaltic pump 60 and being damaged. This constitutes one cycle, and the cells flow back and forth in both directions repeatedly. This method effectively avoids the problem of culture medium splashing and ensures that the culture medium 10 receives a uniform and effective flow rate close to that of blood throughout the entire device, allowing the cells to truly achieve the conditions for suspension culture.

[0028] The above description is only a specific embodiment of this utility model, but the protection scope of this utility model is not limited thereto. Any transformations or substitutions that can be understood by those skilled in the art within the technical scope disclosed in this utility model should be included within the scope of this utility model.

Claims

1. A cell flow suspension culture device simulating blood flow, comprising a culture dish (20) containing culture medium (10), characterized in that, The lower part of the culture dish (20) is connected to the peristaltic pump (60) via a rigid conduit (30); One end of the rigid conduit (30) is connected to the bottom of the culture dish (20), and the other end of the rigid conduit (30) is connected to the soft conduit (50) inside the peristaltic pump (60). The peristaltic pump (60) is equipped with a drive motor for controlling the reciprocating flow of the culture medium (10) in the culture dish (20), hard tubing (30) and soft tubing (50).

2. The cell flow suspension culture device simulating blood flow according to claim 1, characterized in that, The peristaltic pump (60) is equipped with rollers (70) driven by the drive motor to rotate in both the forward and reverse directions. When the rollers (70) rotate, they squeeze the soft tubing (50), so that the culture medium (10) in the culture dish (20), hard tubing (30) and soft tubing (50) reaches a speed similar to that of blood flow.

3. The cell flow suspension culture device simulating blood flow according to claim 1, characterized in that, The rigid catheter (30) is provided with a filter membrane (40) to prevent cells in the culture medium (10) from flowing into the soft catheter (50).

4. The cell flow suspension culture device simulating blood flow according to claim 3, characterized in that, The rigid conduit (30) includes a first rigid conduit (31) and a second rigid conduit (32). One end of the first rigid conduit (31) and the second rigid conduit (32) are respectively connected to the bottom sides of the culture dish (20), and the other end of the first rigid conduit (31) and the second rigid conduit (32) are respectively connected to the two ends of the soft conduit (50).

5. The cell flow suspension culture device simulating blood flow according to claim 4, characterized in that, The first rigid conduit (31) is provided with a first filter membrane (41), and the second rigid conduit (32) is provided with a second filter membrane (42).

6. The cell flow suspension culture device simulating blood flow according to claim 4, characterized in that, The flexible conduit (50) is U-shaped, with both ends of its U-shaped opening extending out of the housing of the peristaltic pump (60) and connected to the first rigid conduit (31) and the second rigid conduit (32).

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