Microfluidic reaction mechanism

Abstract

The utility model discloses a microfluidic reaction mechanism. The microfluidic reaction mechanism comprises a main body frame, the microfluidic reaction chip comprises a lower chip body, an upper chip body is fixed to the upper side of the lower chip body, a liquid inlet hole is formed in the upper chip body, a liquid inlet flow channel and a plurality of amplification pools are arranged at the upward end of the lower chip body, and a plurality of amplification pools are also arranged at the downward end of the upper chip body. The upper chip body and the lower chip body are rotatably connected with a liquid distribution valve body, the liquid distribution valve body is provided with a plurality of liquid outlet flow channels corresponding to the amplification pools one by one, one end of any liquid outlet flow channel can be communicated with the other end of the liquid inlet flow channel, the other end of any liquid outlet flow channel is communicated with the corresponding amplification pool, the upper chip body is provided with a negative pressure hole, and the negative pressure hole is communicated with the amplification pools. The negative pressure hole can be communicated with any amplification pool; the liquid separation assembly comprises a liquid separation regulation and control shaft rotationally connected to the main body frame, and the liquid separation regulation and control shaft can be connected with the liquid separation valve body; according to the utility model, the detection of different viruses can be realized through one micro-fluidic chip, and the operation is convenient.

Description

Technical Field

[0001] This utility model relates to the field of microfluidics technology, and in particular to a microfluidic reaction mechanism. Background Technology

[0002] Microfluidic reaction chips play a crucial role in fields such as biochemical analysis, medical diagnostics, drug development, and environmental monitoring. However, current technologies require a specific number of microfluidic reaction chips to detect different viruses, resulting in high costs and cumbersome procedures. Utility Model Content

[0003] The purpose of this section is to outline some aspects of embodiments of the present invention and to briefly describe some preferred embodiments. Simplifications or omissions may be made in this section, as well as in the abstract and title of this application, to avoid obscuring the purpose of these documents; however, such simplifications or omissions should not be construed as limiting the scope of the present invention.

[0004] In view of the problems mentioned above and / or existing microfluidic detection methods, this utility model is proposed.

[0005] Therefore, the purpose of this invention is to provide a microfluidic reaction mechanism that can detect different viruses through a single microfluidic chip and is easy to operate.

[0006] To solve the above-mentioned technical problems, this utility model provides the following technical solution: a microfluidic reaction mechanism, comprising,

[0007] Main frame;

[0008] A microfluidic reaction chip includes a lower chip body, an upper chip body fixed to the upper side of the lower chip body, an inlet hole on the upper chip body, an inlet channel and several amplification cells at the upward-facing end of the lower chip body, and several amplification cells at the downward-facing end of the upper chip body. A dispensing valve is rotatably connected to the upper and lower chip bodies, and the dispensing valve has several outlet channels corresponding to the amplification cells. One end of any outlet channel can be connected to the other end of the inlet channel, and the other end of any outlet channel can be connected to the corresponding amplification cell. A negative pressure hole is opened on the upper chip body, and the negative pressure hole can be connected to any amplification cell.

[0009] The liquid separation assembly includes a liquid separation control shaft rotatably connected to the main frame, and the liquid separation control shaft can be connected to the liquid separation valve body.

[0010] In a preferred embodiment of the microfluidic reaction mechanism of this utility model, the liquid separation component further includes a lifting rod capable of reciprocating linear motion in the height direction. The lifting rod is connected to the liquid separation control shaft, and the lifting rod can drive the liquid separation control shaft to rise and fall. The liquid separation control shaft is rotatably connected to the lifting rod.

[0011] In a preferred embodiment of the microfluidic reaction mechanism of this utility model, the liquid distribution control shaft has a connecting countersunk hole at its upward-facing end, a limiting shaft is fixed at the lower end of the lifting rod, the limiting shaft can be inserted into the liquid distribution control shaft through the connecting countersunk hole, a rotating sleeve is fitted on the lifting rod, the rotating sleeve abuts against the upper side of the limiting shaft, the rotating sleeve and the liquid distribution control shaft are fixedly connected, and the rotating sleeve is rotatably connected to the lifting rod.

[0012] In a preferred embodiment of the microfluidic reaction mechanism of this utility model, the liquid separation component further includes a drive shaft rotatably connected to the main frame, and the drive shaft and the liquid separation control shaft are connected by a transmission.

[0013] In a preferred embodiment of the microfluidic reaction mechanism of this utility model, a linear actuator is fixedly connected to the upper side of the main frame, and the lifting rod is connected to the linear actuator.

[0014] In a preferred embodiment of the microfluidic reaction mechanism of this utility model, a drive motor is fixedly connected to the upper side of the main frame, and the drive shaft is connected to the drive motor.

[0015] In a preferred embodiment of the microfluidic reaction mechanism of this utility model, a drive wheel is connected to the drive shaft, a driven wheel is connected to the liquid distribution control shaft, the liquid distribution control shaft is slidably connected to the driven wheel, and the drive wheel is connected to the driven wheel via a transmission belt.

[0016] In a preferred embodiment of the microfluidic reaction mechanism of this utility model, a plurality of support parts are fixed on the main frame, a lower support plate is fixedly connected to the lower side of the support parts, an upper support plate is fitted on the support part above the lower support plate, and the upper support plate and the lower support plate are fixedly connected.

[0017] As a preferred embodiment of the microfluidic reaction mechanism in this utility model, the lower support plate is provided with a plurality of lower threaded holes, and the upper support plate is provided with a plurality of upper threaded holes corresponding one-to-one with the lower threaded holes.

[0018] As a preferred embodiment of the microfluidic reaction mechanism in this utility model, wherein: a drain channel is opened on the side of the upper chip body facing downward at the end of the amplification cell away from the dispensing valve body, and a connecting channel is opened at the end of the drain channel away from the amplification cell, which is connected to each drain channel, and the negative pressure hole is connected to the connecting channel.

[0019] Compared with the prior art, this utility model has the following technical effects: the liquid dispensing control shaft, which can be raised, lowered and rotated, achieves a controllable connection with the liquid dispensing valve body. When the liquid dispensing control shaft is connected to the liquid dispensing valve body, it drives the liquid dispensing valve body to rotate, so as to realize the liquid inlet of different amplification cells to complete the amplification reaction. When the liquid dispensing control shaft is detached from the liquid dispensing valve body, it is convenient to remove the microfluidic reaction chip after the amplification reaction and detection are completed, making the operation convenient. Attached Figure Description

[0020] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort. Among them:

[0021] Figure 1 The three-dimensional structure of this utility model Figure 1 .

[0022] Figure 2 The three-dimensional structure of this utility model Figure 2 .

[0023] Figure 3 for Figure 2 A magnified view of a portion of point A in the middle.

[0024] Figure 4 for Figure 2 A magnified view of a section at point B.

[0025] Figure 5 This is a partial internal structural diagram showing the connection between the liquid separation control shaft and the lifting rod in this utility model.

[0026] Figure 6 This is a three-dimensional structural diagram of the microfluidic reaction chip in this utility model.

[0027] Figure 7 The microfluidic reaction chip in this invention is a three-dimensional structure in a transparent state. Figure 1 .

[0028] Figure 8 The microfluidic reaction chip in this invention is a three-dimensional structure in a transparent state. Figure 2 .

[0029] Figure 9 A three-dimensional structural diagram of the lower chip body in this utility model.

[0030] Figure 10 A three-dimensional structural diagram of the chip body in this utility model.

[0031] In the diagram, 100 is the main component, 101 is the connecting bracket, 102 is the connecting seat, 103 is the main frame, 103a is the support part, 104 is the baffle, 105 is the insert plate, 200 is the liquid dispensing assembly, 201 is the drive motor, 202 is the linear actuator, 203 is the lower support plate, 203a is the lower threaded hole, 204 is the upper support plate, 204a is the upper threaded hole, 205 is the liquid dispensing control shaft, 206 is the transmission belt, 207 is the lifting rod, 208 is the rotating sleeve, 209 is the limiting shaft, 209a is the threaded rod, 300 is the microfluidic reaction chip, 301 is the liquid dispensing valve body, 301a is the connecting countersunk hole, 301b is the liquid outlet channel, 302 is the upper chip body, 302a is the liquid inlet, 302b is the negative pressure hole, 302c is the connecting channel, 302d is the drain channel, 303 is the lower chip body, 303a is the liquid inlet channel, 400 is the negative pressure pump, and X-ray amplification cell. Detailed Implementation

[0032] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the specific embodiments of this utility model will be described in detail below with reference to the accompanying drawings.

[0033] Many specific details are set forth in the following description in order to provide a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art can make similar extensions without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0034] Secondly, the term "an embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it a single or selective embodiment that excludes other embodiments.

[0035] Example 1

[0036] Reference Figure 1 , Figure 2 and Figures 6-10 This embodiment provides a microfluidic reaction mechanism that can detect different viruses.

[0037] A microfluidic reaction mechanism includes a main body assembly 100, which includes a connecting seat 102. A main body frame 103 is fixedly connected to the upper side of the connecting seat 102. A connecting bracket 101 is fixedly connected to one side of the connecting seat 102 in the left-right direction. A negative pressure pump 400 is fixedly connected to the connecting bracket 101. A placement trough is opened at the upward end of the connecting seat 102. An insertion port is opened at the left-right end of the placement trough. A microfluidic reaction chip 300 is inserted into the main body frame 103 through the insertion port. The microfluidic reaction chip 300 passes through the insertion port and is inserted into the placement trough. A insertion hole is opened on the main body frame 103 on the side of the insertion port opposite to the placement trough. An insertion plate 105 is inserted into the connecting seat 102 from top to bottom through the insertion hole to prevent the microfluidic reaction chip 300 from moving left and right. A baffle 104 is fixed on the upper side of the connecting seat 102 away from the baffle 104 to prevent the microfluidic reaction chip 300 from moving up and down.

[0038] The microfluidic reaction chip 300 includes a lower chip body 303, an upper chip body 302 fixed to the upper side of the lower chip body 303, an inlet port 302a on the upper chip body 302, an inlet channel 302a on the upper chip body 303, an inlet channel 303a and several amplification cells X on the upper chip body 303's upward-facing end, and several amplification cells X on the lower end of the upper chip body 302. A dispensing valve body 301 is rotatably connected to the upper chip body 302 and the lower chip body 303. The dispensing valve body 301 has several outlet channels 301b corresponding one-to-one with the amplification cells X. One end of any outlet channel 301b can connect with the inlet channel. The other end of 303a is connected, and the other end of any liquid outlet channel 301b is connected to the corresponding amplification cell X. A negative pressure hole 302b is opened on the upper chip body 302. A negative pressure tube is connected to the negative pressure pump 400. The end of the negative pressure tube away from the negative pressure pump 400 is connected to the negative pressure hole 302b. A drain channel 302d is opened on the downward side of the upper chip body 302 at the end of the amplification cell X away from the dispensing valve body 301. A connecting channel 302c is opened at the end of the drain channel 302d away from the amplification cell X, which is connected to each drain channel 302d. The negative pressure hole 302b is connected to the connecting channel 302c.

[0039] The detection solution to be amplified enters the inlet channel 303a through the inlet port 302a. Different amplification cells X store different fluorescent probes. When the inlet channel 303a is connected to a corresponding amplification cell X, the negative pressure pump 400 works to draw the liquid in the inlet channel 303a into the amplification cell X. The detection solution reacts with the fluorescent probe in the amplification cell X to perform amplification. After amplification is completed, subsequent amplification and detection are performed.

[0040] The liquid separation assembly 200 includes a liquid separation control shaft 205 rotatably connected to the main frame 103, and the liquid separation control shaft 205 can be connected to the liquid separation valve body 301.

[0041] In the initial state, the two ends of the outlet channel 301b of the dispensing valve body 301 are isolated from the inlet channel 303a and any amplification cell X, respectively. When it is necessary to connect the inlet channel 303a and the corresponding amplification cell X, the specific process is as follows: connect the dispensing control shaft 205 to the dispensing valve body 301, rotate the dispensing control shaft 205, and the dispensing control shaft 205 drives the dispensing valve body 301 to rotate. When the two ends of the outlet channel 301b are connected to the inlet channel 303a and the corresponding amplification cell X, stop rotating the dispensing control shaft 205, and the negative pressure pump 400... The detection solution enters the corresponding amplification cell X through the inlet channel 303a and the outlet channel 301b. When an appropriate amount of detection solution enters the amplification cell X, the negative pressure pump 400 stops operating. The dispensing control shaft 205 continues to rotate, driving the dispensing valve body 301 to rotate. When the inlet channel 303a is connected to the next amplification cell X, the rotation of the dispensing control shaft 205 stops, the negative pressure pump 400 operates, and an appropriate amount of liquid in the inlet channel 303a is drawn into the next amplification cell X. The negative pressure pump 400 then stops operating. The above steps are repeated until all amplifications are completed.

[0042] The liquid dispensing control shaft 205, which can rotate, is controllably connected to the liquid dispensing valve body 301. When the liquid dispensing control shaft 205 is connected to the liquid dispensing valve body 301, it drives the liquid dispensing valve body 301 to rotate, so as to realize the liquid injection of different amplification cells X to complete the amplification reaction.

[0043] Example 2

[0044] Reference Figures 1-5 This embodiment provides a microfluidic reaction mechanism. The difference between this embodiment and Embodiment 1 is that it can further realize liquid separation within the microfluidic reaction chip 300.

[0045] Specifically, the liquid dispensing assembly 200 also includes a lifting rod 207 capable of reciprocating linear motion in the height direction. The lifting rod 207 is connected to the liquid dispensing control shaft 205, and the lifting rod 207 can drive the liquid dispensing control shaft 205 to rise and fall. The liquid dispensing control shaft 205 is rotatably connected to the lifting rod 207. A connecting countersunk hole 301a is opened at the upward end of the liquid dispensing control shaft 205. A limiting shaft 209 is fixedly connected to the lower end of the lifting rod 207. A threaded rod 209a is fixed on the upper side of the limiting shaft 209. A threaded countersunk hole is opened on the downward side of the lifting rod 207, and the limiting shaft 209 can be inserted into the liquid dispensing control shaft 205 through the connecting countersunk hole 301a. A rotating sleeve 208 is fitted on the lifting rod 207. The rotating sleeve 208 abuts against the upper side of the limiting shaft 209. The rotating sleeve 208 is fixedly connected to the liquid dispensing control shaft 205, and the rotating sleeve 208 is rotatably connected to the lifting rod 207.

[0046] The rotating sleeve 208 has several external and internal connecting holes, which are coaxial. When the dispensing control shaft 205 is connected to the lifting rod 207, the rotating sleeve 208 is fitted onto the lifting rod 207. The threaded rod 209a on the upper side of the limiting shaft 209 is screwed into the threaded countersunk hole on the lower side of the lifting rod 207, thus achieving a fixed connection between the limiting shaft 209 and the lifting rod 207. The dispensing control shaft 205 is then fitted onto the rotating sleeve 208 from bottom to top along the limiting shaft 209. 8. When the liquid distribution control shaft 205 abuts against the lower side of the limit shaft 209, the liquid distribution control shaft 205 cannot continue to move upward. Rotate the rotating sleeve 208 to make the outer connecting hole and the corresponding inner connecting hole coaxial. Use the fixing screw to screw into the outer connecting hole and the corresponding inner connecting hole to fix the liquid distribution control shaft 205 and the rotating sleeve 208 together. The outer circumference of the limit shaft 209 is circular and will not affect the rotation of the liquid distribution control shaft 205, thereby realizing that the liquid distribution control shaft 205 rises and falls with the rise and fall of the lifting rod 207.

[0047] Specifically, the liquid separation assembly 200 also includes a drive shaft rotatably connected to the main frame 103. The drive shaft and the liquid separation control shaft 205 are connected in a transmission connection. A linear actuator 202 is fixedly connected to the upper side of the main frame 103. A lifting rod 207 is connected to the linear actuator 202. A drive motor 201 is fixedly connected to the upper side of the main frame 103. The drive shaft is connected to the drive motor 201. A drive wheel is connected to the drive shaft. A driven wheel is connected to the liquid separation control shaft 205. The liquid separation control shaft 205 is slidably connected to the driven wheel. The drive wheel is connected to the driven wheel via a transmission belt 206.

[0048] When the liquid distribution control shaft 205 needs to be raised or lowered, the linear actuator 202 is activated, causing the lifting rod 207 to rise or fall. The lifting rod 207 drives the liquid distribution control shaft 205 to rise or fall, and the liquid distribution control shaft 205 slides up and down along the transmission wheel. When the liquid distribution control shaft 205 reaches the required height, the linear actuator 202 stops operating. In this application, the linear actuator 202 is preferably an electric push rod. When the liquid distribution control shaft 205 needs to rotate, the drive motor 201 is activated, the drive shaft rotates, and the drive shaft drives the driven wheel to rotate via the drive wheel and the transmission belt 206. The driven wheel drives the liquid distribution control shaft 205 to rotate. When the liquid distribution control shaft 205 rotates to the required angle position, the drive motor 201 stops operating.

[0049] Specifically, a number of support parts 103a are fixed on the main frame 103. A lower support plate 203 is fixedly connected to the lower side of the support parts 103a. An upper support plate 204 is fitted onto the support parts 103a above the lower support plate 203. The upper support plate 204 and the lower support plate 203 are fixedly connected. A number of lower threaded holes 203a are arranged on the lower support plate 203. A number of upper threaded holes 204a corresponding to the lower threaded holes 203a are arranged on the upper support plate 204. By screwing a fixing bolt into the lower threaded holes 203a and the upper threaded holes 204a, the end of the fixing bolt abuts against the lower side of the lower support plate 203. A fixing nut is screwed onto the fixing bolt on the upper side of the upper support plate 204. The fixing nut abuts against the upper side of the upper support plate 204, thus realizing the fixed connection between the lower support plate 203 and the upper support plate 204.

[0050] The upper support plate 204 and the lower support plate 203 provide axial support for the driving wheel and the driven wheel, thereby improving the reliability of the structure in which the driving wheel is connected to the driving shaft and the driven wheel is connected to the liquid distribution control shaft 205.

[0051] The liquid distribution control shaft 205, which can be raised and lowered, connects or disconnects from the liquid distribution valve body 301. The lower part of the liquid distribution control shaft 205 is not perfectly circular. When the liquid distribution control shaft 205 is inserted into the non-circular rotating countersunk hole at the upper end of the liquid distribution valve body 301, the connection between the liquid distribution control shaft 205 and the liquid distribution valve body 301 is achieved. When the liquid distribution control shaft 205 rotates, it drives the liquid distribution valve body 301 to rotate, thereby achieving controllable liquid distribution, which facilitates subsequent... For the continued detection of different viruses, when the dispensing control shaft 205 is lifted upward and detached from the dispensing valve body 301, after the amplification reaction and detection are completed, the microfluidic reaction chip 300 can be pushed through the dispensing valve body 301 to move the microfluidic reaction chip 300 away from the connector 102 to the recycling station, where a new microfluidic reaction chip 300 is placed. After the microfluidic reaction chip 300 is completely placed in the placement tank, the insert plate 105 is inserted to complete the replacement of the microfluidic reaction chip 300. The operation is convenient.

[0052] It should be noted that the above embodiments are only used to illustrate the technical solution of this utility model and are not intended to limit it. Although this utility model has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solution of this utility model without departing from the spirit and scope of the technical solution of this utility model, and all such modifications or substitutions should be covered within the scope of the claims of this utility model.

Claims

1. A microfluidic reaction mechanism, characterized in that: It includes, Main frame (103); A microfluidic reaction chip (300) includes a lower chip body (303), an upper chip body (302) fixed to the upper side of the lower chip body (303), an inlet hole (302a) on the upper chip body (302), an inlet channel (303a) and several amplification cells (X) at the upward-facing end of the lower chip body (303), and several amplification cells (X) at the downward-facing end of the upper chip body (302). The upper chip body (302) and the lower chip body... A liquid dispensing valve body (301) is rotatably connected to the body (303). The liquid dispensing valve body (301) has a plurality of liquid outlet channels (301b) corresponding one-to-one with the amplification cell (X). One end of any liquid outlet channel (301b) can be connected to the other end of the liquid inlet channel (303a), and the other end of any liquid outlet channel (301b) can be connected to the corresponding amplification cell (X). A negative pressure hole (302b) is opened on the upper chip body (302), and the negative pressure hole (302b) can be connected to any amplification cell (X). The liquid separation assembly (200) includes a liquid separation control shaft (205) rotatably connected to the main frame (103), the liquid separation control shaft (205) being able to connect to the liquid separation valve body (301).

2. The microfluidic reaction mechanism as described in claim 1, characterized in that: The liquid separation assembly (200) also includes a lifting rod (207) that can reciprocate linearly in the height direction. The lifting rod (207) is connected to the liquid separation control shaft (205). The lifting rod (207) can drive the liquid separation control shaft (205) to rise and fall. The liquid separation control shaft (205) is rotatably connected to the lifting rod (207).

3. The microfluidic reaction mechanism as described in claim 2, characterized in that: The liquid distribution control shaft (205) has a connecting countersunk hole (301a) at its upward-facing end. The lower end of the lifting rod (207) is fixed with a limiting shaft (209). The limiting shaft (209) can be inserted into the liquid distribution control shaft (205) through the connecting countersunk hole (301a). A rotating sleeve (208) is fitted on the lifting rod (207). The rotating sleeve (208) abuts against the upper side of the limiting shaft (209). The rotating sleeve (208) and the liquid distribution control shaft (205) are fixedly connected. The rotating sleeve (208) is rotatably connected to the lifting rod (207).

4. The microfluidic reaction mechanism as described in claim 2, characterized in that: The liquid separation assembly (200) also includes a drive shaft rotatably connected to the main frame (103), and the drive shaft is drively connected to the liquid separation control shaft (205).

5. The microfluidic reaction mechanism as described in claim 4, characterized in that: A linear actuator (202) is fixedly connected to the upper side of the main frame (103), and the lifting rod (207) is connected to the linear actuator (202).

6. The microfluidic reaction mechanism as described in claim 4, characterized in that: A drive motor (201) is fixedly connected to the upper side of the main frame (103), and the drive shaft is connected to the drive motor (201).

7. The microfluidic reaction mechanism as described in claim 4, characterized in that: The drive shaft is connected to a drive wheel, and the liquid distribution control shaft (205) is connected to a driven wheel. The liquid distribution control shaft (205) is slidably connected to the driven wheel, and the drive wheel is connected to the driven wheel via a transmission belt (206).

8. The microfluidic reaction mechanism as described in claim 7, characterized in that: The main frame (103) is fixed with several support parts (103a). A lower support plate (203) is fixedly connected to the lower side of the support part (103a). An upper support plate (204) is fitted on the support part (103a) above the lower support plate (203). The upper support plate (204) and the lower support plate (203) are fixedly connected.

9. The microfluidic reaction mechanism as described in claim 8, characterized in that: The lower support plate (203) has a plurality of lower threaded holes (203a) arranged on it, and the upper support plate (204) has a plurality of upper threaded holes (204a) that correspond one-to-one with the lower threaded holes (203a) arranged on it.

10. The microfluidic reaction mechanism according to any one of claims 1 to 9, characterized in that: The amplification cell (X) has a drainage channel (302d) on the downward-facing side of the upper chip body (302) at the end away from the dispensing valve body (301). The end of the drainage channel (302d) away from the amplification cell (X) has a connecting channel (302c) that communicates with each drainage channel (302d). The negative pressure hole (302b) is connected to the connecting channel (302c).

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