Electrophoresis apparatus for observing electrophoresis process
By using a modularly designed electrophoresis apparatus, combined with magnetic connections and a guiding mechanism, the problems of existing electrophoresis apparatus being difficult to observe in real time and complex to maintain have been solved, realizing real-time observation of the electrophoresis process and improving the flexibility and ease of maintenance of the equipment.
Patent Information
- Application Number
- CN202422559696.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-23
- Publication Date
- 2025-11-04
- Estimated Expiration
- 2034-10-23
AI Technical Summary
Existing electrophoresis apparatuses are difficult to monitor in real time during the electrophoresis process, and the integrated design of the gel etching device and the electrophoresis apparatus results in inflexible use, high cost, and complex maintenance.
The electrophoresis apparatus features a modular design, comprising an electrophoresis module, an illumination module, and a power module. It is easy to assemble and disassemble using magnetic connectors and banana plugs, and the guide mechanism simplifies the insertion and removal process.
It enables real-time observation of the electrophoresis process, simplifies equipment maintenance and upgrades, and improves equipment flexibility and service life.
Smart Images
Figure CN223513180U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of detection in biomedicine for electrophoretic analysis, and in particular to an electrophoresis apparatus for observing the electrophoretic process. Background Technology
[0002] Electrophoresis is widely used in various fields such as biochemistry, analytical chemistry, clinical chemistry, and immunology. Besides its application in the separation and analysis of small molecules, it is primarily used for the study of proteins, nucleic acids, enzymes, and even viruses and cells. Most existing electrophoresis apparatuses can complete the basic electrophoresis process, but real-time observation and recording during electrophoresis are relatively inconvenient. Typically, after electrophoresis in the electrophoresis tank, the gel needs to be moved to an illumination device for observation, a cumbersome and time-consuming operation. While some existing electrophoresis apparatuses also have illumination devices, these are usually integrated into the apparatus, making the illumination device either impossible to disassemble or complex to assemble. When different illumination sources are required for observation, the entire electrophoresis apparatus often needs to be replaced, resulting in high equipment costs and a lack of flexibility in its use. Utility Model Content
[0003] To address the aforementioned technical problems, this utility model provides an electrophoresis apparatus for observing the electrophoresis process, which facilitates real-time observation during electrophoresis. The modular design makes each part easy to use and maintain, and also more flexible. At the same time, the convenient disassembly and assembly connection between the modules makes the use more efficient.
[0004] The technical solution of this utility model is as follows: it includes an electrophoresis module for performing electrophoresis, a gel etching module disposed below the electrophoresis module, and a power supply module for providing power to the electrophoresis module and the gel etching module; the gel etching module includes a gel etching box, and a gel etching light source is provided in the mounting cavity of the gel etching box, and the gel etching light source is electrically connected to the power supply module through a magnetic connector.
[0005] A further technical solution is as follows: the electrophoresis module includes an electrophoresis tank, and two sets of parallel electrode grooves are opened on the bottom of the two inner sides of the electrophoresis tank. Electrode rods for electrophoresis are respectively housed in the electrode grooves on both sides, and the electrode rods on both sides are respectively connected to the electrode output terminals of the power module.
[0006] A further technical solution is that the end face of the power module opposite to the electrophoresis tank is provided with a banana socket, and the electrode rods on both sides are respectively connected to the corresponding banana sockets through banana plugs.
[0007] A further technical solution is that a guide mechanism is provided between the power module and the electrophoresis tank, and the electrophoresis tank completes the insertion and removal of the banana plug and the banana socket under the guidance of the guide mechanism.
[0008] A further technical solution is that the guiding mechanism includes a guide plate and a guide groove for relative movement of the guide plate, and the guide groove is disposed on the power module.
[0009] A further technical solution is as follows: the photopolymerization light source is a blue light source, and a blue filter plate and a blue light lamp plate are arranged sequentially from top to bottom inside the mounting cavity of the photopolymerization box. The blue light source is set on the blue light lamp plate, and the blue filter plate is located below the electrophoresis module.
[0010] A further technical solution is that the curing light source is an ultraviolet light source, and the mounting cavity of the curing box is equipped with an ultraviolet lamp plate for mounting the ultraviolet light source.
[0011] A further technical solution is as follows: the power module has a circuit board inside, and the two sets of electrode rods are respectively connected to the positive and negative interfaces of the circuit board through banana plugs; the photoresist light source is connected to the corresponding interface on the circuit board through a magnetic connector.
[0012] A further technical solution is that the electrode grooves on both sides are recessed along the bottom of both sides of the electrophoresis tank to form a cavity below the electrophoresis tank, and the photopolymerization module is located in the cavity.
[0013] The beneficial technical effects of this utility model are as follows: by adding a gel illumination module to the electrophoresis apparatus, the separation of the colloids can be observed in real time during the electrophoresis process. Moreover, the connection methods between the gel illumination module and the electrophoresis module and the power supply module are magnetic connectors and banana plugs, respectively, which makes disassembly and replacement simple and convenient. At the same time, the electrophoresis module, gel illumination module and power supply module adopt a modular design, which facilitates independent maintenance and upgrading of each part, effectively improving the service life and flexibility of the equipment. Attached Figure Description
[0014] Figure 1 This is a schematic diagram of the overall structure of this utility model;
[0015] Figure 2 This is a schematic diagram of the end face structure of the electrophoresis module and the photopolymerization module of this utility model relative to the power supply module;
[0016] Figure 3 This is a schematic diagram of the internal structure of the electrophoresis tank of this utility model;
[0017] Figure 4 This is an exploded view of the power module of this utility model;
[0018] Figure 5 This is an exploded view of the adhesive module using a blue light source in this utility model;
[0019] The components include: 1. Electrophoresis module; 11. Electrophoresis tank; 12. Tank cover; 13. Electrode tank; 14. Gel tray; 2. Irradiation module; 21. Irradiation box; 22. Blue filter plate; 23. Blue light board; 3. Power module; 31. Control panel; 32. Circuit board; 4. Banana plug; 5. Guide mechanism; 51. Guide plate; 52. Guide groove; 6. Magnetic connector. Detailed Implementation
[0020] In order to better understand the technical means of this utility model and to implement it in accordance with the contents of the specification, the specific embodiments of this utility model will be further described in detail below with reference to the accompanying drawings and examples. The following examples are used to illustrate this utility model, but are not intended to limit the scope of this utility model.
[0021] like Figure 1 As shown, an electrophoresis apparatus for observing the electrophoresis process includes an electrophoresis module 1, an illumination module 2 disposed below the electrophoresis module 1, and a power supply module 3 that provides power to the electrophoresis module 1 and the illumination module 2. The electrophoresis module 1 serves as the supporting body for the electrophoresis process, and the illumination module 2 is used to irradiate and observe the colloids during the electrophoresis process to observe the separation process in real time.
[0022] like Figure 3 The electrophoresis module 1 includes an electrophoresis tank 11 for electrophoresis, which is made of transparent material to facilitate observation during the experiment. The electrophoresis tank 11 is provided with a tank cover 12. The bottom of the two inner sides of the electrophoresis tank 11 is provided with recessed electrode grooves 13. The two electrode grooves 13 are parallel to each other. Electrode rods for electrophoresis are respectively accommodated in the two electrode grooves 13. The two recessed electrode grooves 13 form a cavity below the electrophoresis tank 11. The photopolymerization module 2 is located in the cavity.
[0023] The electrode rods in the electrode slots 13 on both sides are respectively connected to the electrode output terminals of the power module 3. Specifically, the end face of the power module 3 opposite to the electrophoresis tank 11 is provided with a banana socket, and the electrode rods are respectively plugged into the corresponding banana sockets through banana plugs 4.
[0024] The electrode groove 13 is provided with a gel tray 14 for easy repositioning.
[0025] Furthermore, such as Figure 2A guide mechanism 5 is provided between the power module 3 and the electrophoresis tank 11. The electrophoresis tank 11 moves relative to the power module 3 through the guide mechanism 5, and completes the insertion and removal of the banana plug 4 into the banana socket. Specifically, the guide mechanism 5 includes a guide plate 51 and a guide groove 52 for relative movement of the guide plate 51. The guide plate 51 is disposed on the tank cover 12, and the guide groove 52 is disposed on the power module 3. A magnet is provided on the guide plate 51, and the magnet plays a positioning role after the banana plug 4 is inserted into the banana socket.
[0026] The curing module 2 includes a curing box 21, and a curing light source is installed inside the mounting cavity of the curing box 21. A through hole is provided on the end face of the curing box 21 opposite to the power module 3. The curing light source passes through the through hole via a magnetic connector 6 and is electrically connected to the power module 3. The connection method between the curing module 2 and the power module 3 using the magnetic connector 6 not only ensures the stability of power transmission but also makes the disassembly and replacement of the curing module 2 simpler and more convenient.
[0027] The power module 3 is also equipped with a control panel 31, such as Figure 4 The power module 3 contains a circuit board 32, on which a power adapter and a circuit switch are mounted. The circuit switch is electrically connected to the switch button on the control panel 31, and the power adapter is electrically connected to the power socket on one side of the power module 3. Two sets of electrode rods are respectively connected to the positive and negative terminals of the circuit board 32 via banana plugs 4; the photoresist light source is connected to the corresponding interface on the circuit board 32 via a magnetic connector 6.
[0028] In use, remove the gel tray 14, place the sample into the inner wall of the gel tray 14, then put the gel tray 14 back into the electrophoresis tank 11 in the homogeneous electrolyte, and cover the tank with the tank cover 12; after connecting the electrophoresis module 1 and the gel imaging module 2 to the power module 3 respectively, turn on the switch button on the control panel 31. Under the action of the electric field, the colloidal particles move directionally towards the cathode or anode in the electrolyte. The electrophoretic bands can be observed in real time during the electrophoresis process through the gel imaging module 2, without the need to remove and transfer the gel to the gel imaging instrument for imaging and photography after the electrophoresis reaction is completed; if only electrophoresis is performed, simply connect the electrophoresis module 1 to the power module 3 and turn on the switch button.
[0029] In one embodiment, when a blue light source is required for electrophoresis observation, such as... Figure 5 The mounting cavity of the electrophoresis tank 21 is provided with a blue filter plate 22 and a blue light lamp plate 23 from top to bottom. The blue light source is set on the blue light lamp plate 23. The blue filter plate 22 is a blue acrylic plate and is located below the electrophoresis tank 11.
[0030] In another real-time example, when an ultraviolet light source is required for electrophoresis observation, the mounting cavity of the gel illumination box 21 is equipped with an ultraviolet lamp plate for mounting the ultraviolet light source. The electrophoresis observation requirements of different samples can be met simply by replacing the corresponding gel illumination module 2.
[0031] This structure combines the gel etching module 2 and the electrophoresis module 1 into a compact and uniform design, reducing the size and avoiding the tedious steps of moving the gel multiple times. At the same time, the modular design of the electrophoresis module 1, gel etching module 2, and power supply module 3 facilitates independent maintenance and updates of each part, effectively improving the service life and flexibility of the equipment.
[0032] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of the present utility model, and these improvements and modifications should also be considered within the protection scope of the present utility model.
Claims
1. An electrophoresis apparatus for observing the electrophoresis process, characterized in that: It includes an electrophoresis module (1) for performing electrophoresis, a gel etching module (2) disposed below the electrophoresis module (1), and a power supply module (3) for providing power to the electrophoresis module (1) and the gel etching module (2); the gel etching module (2) includes a gel etching box (21), and a gel etching light source is provided in the mounting cavity of the gel etching box (21). The gel etching light source is electrically connected to the power supply module (3) through a magnetic connector (6).
2. The electrophoresis apparatus for observing the electrophoresis process according to claim 1, characterized in that: The electrophoresis module (1) includes an electrophoresis tank (11). Two sets of parallel electrode grooves (13) are provided on the bottom of the two inner sides of the electrophoresis tank (11). Electrode rods for electrophoresis are respectively housed in the electrode grooves (13) on both sides. The electrode rods on both sides are respectively connected to the electrode output terminals of the power supply module (3).
3. An electrophoresis apparatus for observing the electrophoresis process according to claim 2, characterized in that: The power module (3) is provided with a banana socket on the end face opposite to the electrophoresis tank (11), and the electrode rods on both sides are respectively connected to the corresponding banana sockets through banana plugs (4).
4. An electrophoresis apparatus for observing the electrophoresis process according to claim 3, characterized in that: A guide mechanism (5) is provided between the power module (3) and the electrophoresis tank (11). Under the guidance of the guide mechanism (5), the electrophoresis tank (11) completes the insertion and removal of the banana plug (4) into the banana socket.
5. An electrophoresis apparatus for observing the electrophoresis process according to claim 4, characterized in that: The guiding mechanism (5) includes a guide plate (51) and a guide groove (52) for relative movement of the guide plate (51), and the guide groove (52) is disposed on the power module (3).
6. An electrophoresis apparatus for observing the electrophoresis process according to claim 1, characterized in that: The photocoating light source is a blue light source. The mounting cavity of the photocoating box (21) is provided with a blue filter plate (22) and a blue light lamp plate (23) from top to bottom. The blue light source is set on the blue light lamp plate (23), and the blue filter plate (22) is located below the electrophoresis module (1).
7. An electrophoresis apparatus for observing the electrophoresis process according to claim 1, characterized in that: The light source for the curing process is an ultraviolet light source, and the mounting cavity of the curing box (21) is equipped with an ultraviolet lamp plate for mounting the ultraviolet light source.
8. An electrophoresis apparatus for observing the electrophoresis process according to claim 2, characterized in that: The power module (3) has a circuit board (32) inside. Two sets of electrode rods are connected to the positive and negative interfaces of the circuit board (32) through banana plugs (4); the photoresist light source is connected to the corresponding interface on the circuit board (32) through a magnetic connector (6).
9. An electrophoresis apparatus for observing the electrophoresis process according to claim 2, characterized in that: The electrode grooves (13) on both sides are recessed along the bottom of the electrophoresis tank (11) to form a cavity below the electrophoresis tank (11), and the photopolymerization module (2) is located in the cavity.