Automatic filtration and high-pressure separation and purification equipment and automatic separation and purification preparation system

Abstract

The utility model provides automatic filtering and high-pressure separation and purification equipment and an automatic separation and purification preparation system. The automatic filtration and high-pressure separation and purification equipment comprises a workbench; the first filtering module, the second filtering module, the high-pressure sample inlet and outlet preparation module, the pump valve analysis module and the carrying mechanism are arranged on the workbench; the mobile phase cache module, the first filtering module and the second filtering module are arranged independent of the workbench and are used for filtering the sample liquid according to the first filtering requirement and the second filtering requirement respectively; the high-pressure preparation sample inlet and outlet module is used for introducing the filtered sample liquid, carrying out fraction collection, and enabling the sample liquid and products after fraction collection to automatically enter and exit through a carrying mechanism; the carrying mechanism is used for transferring materials among the first filtering module, the second filtering module and the high-pressure sample inlet and outlet preparation module; the mobile phase cache module is used for caching and placing a mobile phase; and the pump valve analysis module is connected with the high-pressure sample inlet and outlet preparation module and the mobile phase buffer module and is used for carrying out liquid pumping and analysis treatment. According to the invention, full-process automation of filtration and high-pressure preparation is realized.

Description

TECHNICAL FIELD

[0001] The present disclosure relates to the technical field of automation equipment, in particular to the technical field of medical and chemical automation equipment, and more particularly to an automatic filtration and high-pressure separation and purification equipment and an automatic separation and purification preparation system. BACKGROUND

[0002] Experiments in biological, medical, chemical and other industries often involve a large number of filtration, component separation, pipetting, and sub-packaging steps. At present, each step is mainly operated manually, and a large number of operators are required to participate in the process. This not only has low processing efficiency, but also has large errors. Since the operators need to directly contact harmful chemicals or biological samples, there is also a potential health risk. CONTENT OF THE UTILITY MODEL

[0003] In view of the above problems, the present disclosure provides an automatic filtration and high-pressure separation and purification equipment and an automatic separation and purification preparation system, which can realize fully automatic filtration, separation, pipetting, and sub-packaging steps, greatly improving the sample processing speed, reducing human errors, and improving the accuracy and safety of experiments.

[0004] In a first aspect, the present disclosure provides an automatic filtration and high-pressure separation and purification equipment, comprising: a workbench; a first filtration module, a second filtration module, a high-pressure preparation sample import and export module, a pump valve analysis module, and a carrying mechanism arranged on the workbench; and a mobile phase buffer module arranged independently of the workbench, the first filtration module filters sample liquid according to first filtration requirements; the second filtration module filters sample liquid according to second filtration requirements; the high-pressure preparation sample import and export module imports sample liquid filtered by the first filtration module or the second filtration module, collects fractions, and enables the sample liquid and the product after fraction collection to be automatically imported and exported through the carrying mechanism; the carrying mechanism is used to transfer materials in the first filtration module, the second filtration module, and the high-pressure preparation sample import and export module; the mobile phase buffer module is used to buffer and place mobile phase; and the pump valve analysis module is connected with the high-pressure preparation sample import and export module and the mobile phase buffer module respectively to perform pump liquid and analysis processing.

[0005] In some embodiments, the automatic filtration and high-pressure separation and purification equipment further comprises an interaction unit for material interaction with the outside through the carrying mechanism; and a pipetting module for transferring the sample liquid after fraction collection to a predetermined container according to a predetermined pipetting capacity.

[0006] In some embodiments, the pump valve analysis module imports sample liquid from the high-pressure preparation sample import and export module, imports mobile phase corresponding to the sample liquid from the mobile phase buffer module, performs component separation and analysis on the mixed liquid of the sample liquid and the mobile phase, and then exports different components to the high-pressure preparation sample import and export module.

[0007] In some embodiments, the automated filtration and high-pressure separation and purification device further comprises a storage rack for placing materials required when each module is processed, the interaction unit, the storage rack, the first filtration module, the second filtration module, the high-pressure preparation sample in-out module, and the pipetting module are arranged around the carrying mechanism and within the operating range of the carrying mechanism.

[0008] In some embodiments, the mobile phase buffer module comprises a plurality of mobile phase buffer boxes in which large-capacity barrels containing mobile phase are placed.

[0009] In some embodiments, the workbench comprises a front side, a rear side, a left side and a right side, the interaction unit is arranged on the front side, the storage rack is arranged on the left side, the first filtration module, the second filtration module, and the pipetting module are arranged side by side on the rear side, the high-pressure preparation sample in-out module is arranged between the front side and the rear side and closer to the front side, and the pump valve analysis module is arranged adjacent to the high-pressure preparation sample in-out module and away from the carrying mechanism.

[0010] In some embodiments, the first filtration module is a positive pressure filtration module for filtering a small amount of sample liquid, and the second filtration module is a filter screen filtration module for filtering a large amount of sample liquid.

[0011] In some embodiments, the positive pressure filtration module comprises a clamping mechanism for clamping a filter container containing a sample to be filtered, an air inlet connector located above the clamping mechanism and connected to an air source for communicating the air source with the sample to be filtered during filtration, a first lifting mechanism connected to the air inlet connector for driving the air inlet connector to lift, a second lifting mechanism on which the clamping mechanism and the first lifting mechanism are fixed, the second lifting mechanism being used to drive the clamping mechanism, the air inlet connector, and the first lifting mechanism to lift together, a plug removal mechanism for removing a plug below the filter container, and a receiving bottle placement mechanism for placing a receiving bottle receiving filtered sample liquid.

[0012] In some embodiments, the positive pressure filtration module further comprises a base on which the plug removal mechanism and the receiving bottle placement mechanism are arranged, and a support vertically arranged on the base, the first support plate and the second support plate are arranged on the support, the first support plate and the second support plate are arranged perpendicularly, the second support plate is fixed on the first support plate and can move along the first support plate, and the second lifting mechanism is fixedly connected to the second support plate.

[0013] In some embodiments, the filter screen filtering module comprises: a vertical support; a receiving bottle positioning rack arranged at the bottom of the support, used for placing a receiving bottle for receiving filtered sample liquid; a filter assembly support rack arranged above the receiving bottle positioning rack and fixed on the support, used for placing a filter assembly with a filter screen; a lifting mechanism arranged on the support; and a sealing pressure plate connected with the lifting mechanism, the lifting mechanism drives the sealing pressure plate to lift and lower, and the sealing pressure plate is provided with an air inlet connector connected with an air source, used for communicating the air source with the filter assembly during filtering.

[0014] In some embodiments, the lifting mechanism comprises a lifting assembly, a linear guide rail and a motor, the linear guide rail is fixed at an upper position of the support, the lifting assembly is connected with the sealing pressure plate and is driven by the motor to move up and down along the linear guide rail.

[0015] In some embodiments, the high-pressure preparation sample inlet and outlet module comprises: a fraction collection unit comprising a sample inlet needle and a sample outlet needle, used for fraction collection of imported sample liquid; a fraction collection pulling unit comprising a sample inlet position corresponding to the sample inlet needle, a fraction collection position corresponding to the sample outlet needle, and an assembly capable of pulling out or pushing in the sample inlet position and the fraction collection position from the fraction collection unit; and a fraction collection lifting unit used for lifting the fraction collection position.

[0016] In some embodiments, the sample inlet needle of the fraction collection unit is arranged to be liftable, and the sample inlet position of the fraction collection pulling unit is arranged to be fixed; the sample outlet needle of the fraction collection unit is arranged to be fixed, and the fraction collection position of the fraction collection pulling unit is arranged to be liftable.

[0017] In some embodiments, the fraction collection pulling unit comprises: a sample inlet position used for placing a sample to be processed; a fraction collection position used for collecting fractions; a first sliding rail connected with the sample inlet position, the sample inlet position being capable of moving along the first sliding rail; a bottom plate; a plurality of second sliding rails fixed on the bottom plate, the second sliding rails being arranged corresponding to the fraction collection position, the fraction collection position being capable of moving along the second sliding rails; and an end of the fraction collection position being provided with a pulling plate, by applying force to the pulling plate, the fraction collection position is capable of moving along the second sliding rails.

[0018] In some embodiments, a limiting block is arranged on the second sliding rail, and a limiting groove is arranged on the fraction collection position, when the fraction collection position is pulled out, the fraction collection position is fixed on the second sliding rail by the limiting block and the limiting groove.

[0019] In some embodiments, the fraction collection lifting unit is arranged below the bottom plate, and includes a driving motor and a lifting rod connected to the bottom plate, the driving motor drives the lifting rod to lift, the fraction collection position and the sample injection position are respectively provided with a protruding piece and a clamping hole, when the fraction collection position is lifted, the protruding piece is separated from the clamping hole, and the fraction collection position and the sample injection position are separated, when the fraction collection position is lowered, the protruding piece is inserted into the clamping hole, and the fraction collection position and the sample injection position are connected.

[0020] In some embodiments, the high-pressure preparation sample injection and outjection module includes a group of sample injection positions and three groups of fraction collection positions.

[0021] In some embodiments, the pipetting module includes a support including a support vertical plate and a support horizontal plate connected to each other, a pipetting assembly arranged on the support vertical plate, the pipetting assembly being used to detachably connect with a suction head and drive the suction head to suck or spit liquid, and a head removal assembly arranged on the support horizontal plate, the head removal assembly being used to push the suction head to separate from the pipetting assembly.

[0022] In some embodiments, the interaction unit includes a bottom plate arranged on the workbench, support plates connected to both ends of the bottom plate and arranged vertically, and a placement plate connected between the support plates and used to store material containers and / or trays, the placement plate being provided with a plurality of first storage positions, each of the first storage positions including a positioning piece used to limit a material container and / or a tray and a sensor used to detect whether the storage position stores a material container and / or a tray.

[0023] In some embodiments, the carrying mechanism includes a carrying arm and a carrying tool, the carrying arm being a mechanical arm or a moving mechanism having at least one degree of freedom in a direction, one end of the carrying arm being fixed to the workbench, and the other end of the carrying arm being provided with the carrying tool, the carrying tool being detachably connected to the carrying arm.

[0024] In some embodiments, the carrying arm is any one of a four-axis mechanical arm, a six-axis mechanical arm, and an XYZ three-axis linear moving mechanism, and the carrying tool is a container gripper or a tray gripper.

[0025] In some embodiments, the workbench is further provided with a tool storage for storing a plurality of carrying tools, and the carrying mechanism takes and places the carrying tools from the tool storage.

[0026] In some embodiments, the storage rack includes at least one placement plate and two support pieces arranged vertically, the placement plate is arranged between the two support pieces, and the placement plate is provided with second storage positions for placing containers and / or trays.

[0027] In some embodiments, a cover is further arranged on the workbench, and an internal containing space is formed between the cover and the workbench. An interaction window is arranged on the cover at one side of the interaction unit, and is used for material interaction with the external space. Maintenance windows are arranged on the cover at the remaining sides of the cover, and are used for maintenance of the modules in the internal containing space.

[0028] In some embodiments, the interaction window is a sliding window, and the maintenance window is a casement window.

[0029] In a second aspect, the embodiments of the present disclosure provide an automatic separation and purification preparation system, which comprises a mobile device and the automatic filtration and high-pressure separation and purification device of the first aspect. The mobile device is used for conveying materials to the automatic filtration and high-pressure separation and purification device and taking away samples processed by the automatic filtration and high-pressure separation and purification device.

[0030] The present disclosure can select a suitable filtration mode according to the amount of the sample to be filtered by providing the automatic filtration and high-pressure separation and purification device and the automatic separation and purification preparation system. The whole processing process is fully automated, which can reduce the contact between samples and reduce the risk of cross contamination. In addition, the operator does not need to directly contact harmful chemicals or biological samples, which reduces the potential health risk. Moreover, the automatic system can accurately control the amount of reagent used, which reduces waste, especially when expensive reagents are used. BRIEF DESCRIPTION OF DRAWINGS

[0031] Figure 1 is a top view of the automatic filtration and high-pressure separation and purification device of an embodiment of the present disclosure without a cover;

[0032] Figure 2 is a perspective view of the automatic filtration and high-pressure separation and purification device of an embodiment of the present disclosure without a cover;

[0033] Figure 3 is a perspective view of the positive pressure filtration module of an embodiment of the present disclosure;

[0034] Figure 4 is an enlarged view of a part of the positive pressure filtration module of an embodiment of the present disclosure;

[0035] Figure 5 is a perspective view of the filter screen filtration module of an embodiment of the present disclosure;

[0036] Figure 6 is a rear view of the filter screen filtration module of an embodiment of the present disclosure;

[0037] Figure 7 is a perspective view of the high-pressure preparation sample inlet and outlet module of an embodiment of the present disclosure;

[0038] Figure 8is a perspective view of a puller assembly and a lift assembly of an embodiment of the present disclosure;

[0039] Figure 9 is a side view of a puller assembly and a lift assembly of an embodiment of the present disclosure;

[0040] Figure 10 is a perspective view of a pipetting module of an embodiment of the present disclosure;

[0041] Figure 11 is a perspective view of one of the mobile phase buffer tanks in a mobile phase buffer module of an embodiment of the present disclosure;

[0042] Figure 12 is a perspective view of an automated filtration and high pressure separation and purification apparatus with a cover in an open state from a front view of an embodiment of the present disclosure;

[0043] Figure 13 is a perspective view of an automated filtration and high pressure separation and purification apparatus with a cover in an open state from a back view of an embodiment of the present disclosure. DETAILED DESCRIPTION

[0044] In order to make the skilled in the art better understand the technical solutions of the present disclosure, the technical solutions of the present disclosure are described in detail below with reference to the drawings.

[0045] In the following, example embodiments will be described more fully with reference to the accompanying drawings, in which example embodiments can be embodied in different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided as a full and enabling disclosure of the present disclosure, and to fully convey the scope of the present disclosure to the skilled in the art.

[0046] In the case of no conflict, each embodiment of the present disclosure and each feature in the embodiments can be combined with each other.

[0047] As used herein, the term "and / or" includes any and all combinations of one or more of the associated listed items.

[0048] The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the present disclosure. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It will be further understood that the terms "comprises" and / or "comprising," when used in this specification, specify the presence of stated features, integers, steps, operations, elements, and / or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and / or groups thereof.

[0049] The embodiments described herein can be described with reference to plan views and / or cross-sectional views by virtue of the idealized illustrative representations of the disclosed subject matter. Accordingly, the example illustrations can be modified according to manufacturing techniques and / or tolerances. Therefore, embodiments are not limited to the illustrated examples and include modifications based on manufacturing processes. Thus, the shapes of the regions illustrated in the figures are schematic and the shapes of the regions illustrated in the figures do not necessarily illustrate the exact shape of the regions in a device, but are meant to be illustrative and not limiting.

[0050] Unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant art and the present disclosure and will not be interpreted in an overly literal or overly formal sense unless expressly so defined herein.

[0051] Some embodiments of the present disclosure are described in detail below with reference to the accompanying drawings. Reference Figure 1 and Figure 2 , Figure 1 is a top view of an automated filtration and high pressure separation and purification apparatus according to an embodiment of the present disclosure, Figure 2 is a perspective structural schematic view of an automated filtration and high pressure separation and purification apparatus according to an embodiment of the present disclosure. The automated filtration and high pressure separation and purification apparatus 100 includes a workbench 11 on which a plurality of processing modules are disposed, which will be described in detail below. The workbench 11 includes a first side, i.e., a front side 111, a second side, i.e., a rear side 112, a third side, i.e., a left side 113, and a fourth side, i.e., a right side 114. Among them, the front side 111 refers to the side close to the user in the natural use state. The rear side 112 is opposite to the front side 111, which refers to the side away from the user in the use state. The left side 113 refers to the left side of the user in the use state. The right side 114 is opposite to the left side 111, which refers to the right side of the user in the use state.

[0052] The interaction unit 1 is disposed near the first side, i.e., the front side 111 of the workbench 11 and close to the third side, i.e., the left side 113, for interacting with external materials. For example, the material containers and / or trays delivered by the mobile device, such as the external AGV trolley, and the sample containers and / or trays processed in the system are placed in the interaction unit 1 and delivered to other processing systems by the mobile device.

[0053] In some embodiments, the sample is a biological sample, a medical sample, or a chemical sample. When the sample is a medical sample, it can be a western medicine sample, a traditional Chinese medicine sample, or other medicine samples with medicinal effects, etc. For example, the traditional Chinese medicine sample includes traditional Chinese medicinal materials, traditional Chinese medicine decoction pieces, Chinese patent medicines, semi-finished products of traditional Chinese medicines, medicinal plants, etc.

[0054] The interaction unit 1 comprises a bottom plate 105 arranged on the workbench 11, a support plate 104 connected to the opposite ends of the bottom plate 105 and arranged vertically, and a placing plate 103 connected to the support plate 104, the placing plate 103 being used to store material containers and / or trays. The placing plate 103 is provided with at least two first storage positions for storing the material containers and / or trays, each first storage position comprising a set of positioning members 101 and a sensor 102. The positioning members 101 can be provided in two, which can be protrusions or grooves, etc. The bottom of the material container and / or tray is correspondingly provided with a mechanism capable of being clamped with the protrusions or grooves, so as to limit the material container and / or tray by the positioning members 101. The positioning members 101 and the sensor 102 are connected to the placing plate 103, the positioning members 101 being used to limit the material container and / or tray in the storage position, and the sensor 102 being used to sense whether the storage position stores the material container and / or tray. Optionally, a plurality of layers of placing plates 103 can be arranged at intervals, and at least two first storage positions are arranged on each layer of placing plates 103. In this way, the interaction unit 1 has a plurality of first storage positions, and a plurality of storage positions can take and place the material containers and / or trays at the same time, so as to improve the efficiency.

[0055] The carrying mechanism 20 is arranged at a position closer to the second side edge, i.e., the rear side edge 112, of the workbench 11, and close to the third side edge, i.e., the left side edge 113. The carrying mechanism 20 is used to transfer the material between the processing modules, and therefore, the carrying mechanism 20 needs to be arranged at a position having sufficient space for movement, and convenient for interaction with the processing modules to be transferred. The position of the carrying mechanism 20 is not specifically limited, as long as the above conditions are met.

[0056] Optionally, the carrying mechanism 20 comprises a carrying arm 3 and a carrying tool 4 mounted at one end of the carrying arm 3. The carrying arm 3 can be a mechanical arm or a moving mechanism with at least one degree of freedom, specifically, the carrying arm 3 can be a multi-degree-of-freedom mechanical arm, such as a four-axis mechanical arm, a six-axis mechanical arm, etc., or an XYZ three-axis linear moving mechanism. One end of the carrying arm 3 is fixed on the workbench 11, and the other end is provided with the carrying tool 4, which is detachably connected with the carrying arm 3. The carrying tool 4 can be an electric gripper, and different carrying tools 4, such as container grippers, tray grippers, etc., are switched according to the tray or container to be transferred. A tool warehouse is arranged near the position of the carrying mechanism 20, and a plurality of carrying tools 4 are stored in the tool warehouse. Optionally, in an embodiment, a quick-change male head is arranged on the carrying arm 3, and a quick-change female head is arranged on each carrying tool 4, and the quick-change male head and the quick-change female head are matched to realize the detachable connection between the carrying arm 3 and the carrying tool 4. The quick-change male head and the quick-change female head can be quickly connected in a manner of pneumatic connection, magnetic attraction, etc., which is not limited herein. The carrying arm 3 drives the carrying tool 4 to move and clamp the corresponding container or tray and transfer it to the target position.

[0057] Optionally, at least one storage rack 2 can also be arranged on the workbench 11. Figure 1 and 2 In the embodiment shown, two storage racks 2 are arranged on the workbench 11. One of the storage racks 2 is arranged at a position close to the third side edge, i.e., the left side edge 113, between the left side edge 113 and the carrying mechanism 20, and the other storage rack 2 is arranged between the carrying mechanism 20 and the high-pressure preparation sample in-out module 5. When the material containers and / or trays transported by the mobile device are too many to be accommodated by the interactive unit 1 at one time, the material containers and / or trays can be temporarily stored on the storage rack 2. For example, in the preparation stage of the experiment, the mobile device transfers various materials required for the experiment, such as a tray containing empty test tubes, a filter head tray, a Tip head tray, an empty liquid phase vial tray, etc., to the interactive unit 1, and the carrying mechanism 20 transfers the various trays on the interactive unit 1 to the storage rack 2. During the experiment, the samples processed by the filter module and the high-pressure preparation sample in-out module can also be temporarily stored on the storage rack 2, and then transferred to the next processing module by the carrying mechanism 20. After all the processing of the samples is completed, the containers and / or trays on the storage rack 2 are transferred to the interactive unit 1 and then transported to other processing systems by the mobile device. The storage rack 2 is used to place trays, containers, etc., and the containers include but are not limited to test tubes, solvent bottles, etc. For reference Figure 2The storage rack 2 comprises at least one layer of storage plates 202 and two opposite vertical supports 201, and the storage plates 202 are arranged between the two supports 201. The storage plates 202 are provided with second storage positions for placing containers and / or trays. The second storage positions are provided with fixing members 203 for fixing the containers and / or trays to the storage plates 202. The storage plates 202 can be arranged in multiple layers to store different amounts and / or different types of materials according to different requirements. For example, one layer is used to place containers and one layer is used to place trays.

[0058] Optionally, the automated filtration and high-pressure separation and purification device 100 further comprises a detection assembly which can be arranged on the conveying mechanism 20 and used to detect whether there are material containers and / or trays on the interactive unit 1 or the storage rack 2 and detect the materials contained in the material containers and / or trays. The detection assembly can comprise various detection members and identification members, which are not limited herein. The detection assembly can detect and identify the material containers and / or trays and the materials by using any feasible detection principle and identification principle, so as to facilitate the management of the transfer of materials between modules and the processing of materials in the modules.

[0059] Optionally, the automated filtration and high-pressure separation and purification device 100 further comprises a control system which is electrically connected with the detection assembly and used to receive and manage the information detected by the detection assembly. The control system is a control background of the automated filtration and high-pressure separation and purification device 100 and can manage the material operation of the automated filtration and high-pressure separation and purification device 100. The control system can be composed of hardware such as a computer and appropriate software, and the embodiments of the present application do not make specific limitations thereon. The electrical connection between the control system and the detection assembly can be wired or wireless connection, which is not limited herein. The information detected by the detection assembly is input to the control system, the control system can process the information and obtain the state and position of the material operation, and according to the processing requirements, the working process of the automated filtration and high-pressure separation and purification device 100 is scheduled, which is not limited in the embodiments of the present application.

[0060] Reference Figure 1 and Figure 2, the first filter module 7 and the second filter module 8 are arranged side by side near the second side, i.e., the rear side 112 of the workbench 11, and close to the conveying mechanism 20. The first filter module 7 and the second filter module 8 are used for filtering the sample liquid, and the filtered sample liquid is used for the high-pressure preparation sample in-out module 5 or is delivered to other processes by the mobile device. In the embodiment, the first filter module 7 and the second filter module 8 correspond to different filtering requirements. The first filter module 7 is a positive pressure filter module, which is mainly used for filtering a small amount of sample liquid, such as the sample liquid contained in the filter column. The second filter module 8 is a filter screen module, which is mainly used for filtering a large amount of sample liquid, such as the sample liquid contained in the concentrate bottle. It can be understood that the embodiment does not limit the arrangement of two filter modules, nor the type of filter module. One or more filter modules can be arranged according to the filtering requirements, and the filter module can be a filter screen module or other types of filter modules.

[0061] Figure 3 is a perspective view of the positive pressure filter module 7 of an embodiment of the present disclosure, Figure 4 is a partial enlarged view of the vicinity of the filter clamping jaw of the positive pressure filter module 7 of an embodiment of the present disclosure. Referring to Figure 3 and Figure 4 The positive pressure filter module 7 includes a base 701, and the base 701 is provided with a moving mechanism 711, which can move the base plate 705 in a first direction, which can be a direction parallel to the base 701. The base plate 705 is provided with a tray for placing a liquid receiving bottle 713, a tray for placing a filter head, a pulling mechanism 712, and the like. The base 701 is vertically provided with a support 702, and the support 702 is provided with a first support plate 703 and a second support plate 704. The first support plate 703 and the second support plate 704 are perpendicular to each other, and the second support plate 704 is fixed on the first support plate 703 and can move on the first support plate 703 in a second direction, which is perpendicular to the first direction.

[0062] The second lifting mechanism 707 is fixedly connected to the second support plate 704 and can move up and down on the second support plate 704 through a driving mechanism. The first lifting mechanism 706 is further fixedly connected to the second lifting mechanism 707, and the first lifting mechanism 706 is fixedly connected with the air inlet joint 709 and is used to drive the air inlet joint 709 to lift. The air inlet joint 709 is connected with an air source not shown in the figure, and the air source supplies gas to the air inlet joint 709. The filter clamping jaw 708 is also fixedly connected to the second lifting mechanism 707 and is used to clamp a container containing liquid to be filtered, such as a filter column 710. The second lifting mechanism 707 drives the filter clamping jaw 708, the air inlet joint 709, and the first lifting mechanism 706 to lift together, and the filter clamping jaw 708, the air inlet joint 709, and the first lifting mechanism 706 can also move on the first support plate 703 in the second direction.

[0063] The filter column 710 contains the sample liquid to be filtered, and a plug is arranged below the filter column 710 to prevent the liquid from leaking out before being filtered. The filter column 710 can also be provided with a filter head before being provided with the plug. A pulling mechanism 712 for pulling out the plug is arranged on the bottom plate 705, and the pulling mechanism 712 is provided with a pulling plate having a clamping groove. A recovery mechanism is arranged below the pulling mechanism 712. The filter clamping jaw 708 can move the filter column 710 to the position of the pulling mechanism 712, and then the filter clamping jaw 708 lowers the filter column 710 to clamp the plug below the filter column 710 in the clamping groove. Then the filter clamping jaw 708 moves the filter column 710 upward, and the plug is pulled out and falls into the recovery mechanism below.

[0064] When filtering, the filter clamping jaw 708 clamps the filter column 710, the first lifting mechanism 706 drives the air inlet connector 709 to lower, and the air inlet connector 709 is connected to the filter column 710. The second support plate 704 moves the filter clamping jaw 708 and the filter column 710 to above the pulling mechanism 712, and the second lifting mechanism 707 drives the filter clamping jaw 708 to lower while clamping the filter column 710. The plug of the filter column 710 is clamped in the clamping groove of the pulling mechanism 712. Then the second lifting mechanism 707 drives the filter clamping jaw 708 and the filter column 710 to rise, and the plug of the filter column 710 is pulled out.

[0065] When the filter column 710 is provided with a filter head, the filter head does not need to be pierced. When the filter column 710 is not provided with a filter head, the second support plate 704 moves the filter clamping jaw 708 and the filter column 710 to the position of the filter head, and the filter head is pierced. If the sample volume is 0-2 ml, a Tip head is needed to be pierced at the liquid outlet of the filter head to puncture and filter the sample into a liquid phase vial. If the sample volume is 2-30 ml, a Tip head does not need to be pierced, and the sample is directly filtered into a test tube. Then the second support plate 704 moves the filter clamping jaw 708 and the filter column 710 to above the liquid receiving bottle 713, and the second lifting mechanism 707 drives the filter clamping jaw 708 to lower while clamping the filter column 710. The filter column 710 is connected to the liquid receiving bottle 713, and then the gas source is turned on. The air inlet connector 709 connects the gas source to the filter column 710 to introduce gas pressure into the filter column 710, and the sample liquid in the filter column 710 is filtered by the gas pressure. After the filtration is completed, the recovery mechanism is moved to directly below the filter clamping jaw 708 by the moving mechanism 711. The first lifting mechanism 24 drives the air inlet connector 709 to separate from the filter column 710, the filter clamping jaw 708 releases the filter column 710, and the filter column 710 and the filter head fall into the recovery mechanism.

[0066] Figure 5 is a perspective view of a filter screen filtering module according to an embodiment of the present disclosure, Figure 6is a back view of the filter screen filtration module according to an embodiment of the present disclosure. Reference is made to Figure 5 and Figure 6 The filter screen filtration module 8 comprises a vertical support 801, a bottom plate 802 arranged at the bottom of the support 801, a filter assembly support 803 arranged at a lower central position of the support 801, a receiving bottle positioning rack 804 arranged at a position close to the bottom of the support 801, a lifting mechanism 805 arranged at an upper position of the support 801, the lifting mechanism 805 comprising a linear guide rail 806, a lifting assembly 807 and a lifting motor 808 arranged at the back of the support 801, the lifting motor 808 driving the lifting assembly 807 to move up and down along the linear guide rail 806. The lifting assembly 807 is connected with a sealing pressure plate 809, and drives the sealing pressure plate 809 to move up and down, and a spring 813 is arranged in the lifting assembly 807 for buffering pressure when moving up and down. An air inlet connector 816 is arranged on the sealing pressure plate 809, the air inlet connector 816 is connected with an air pipe 810, the air pipe 810 is connected with an air source not shown in the figure, and a pressure regulating valve 811 and a throttle valve 812 are further arranged on the air pipe 810 for adjusting the pressure of the gas in the air pipe 810, and the air pipe 810 is fixed on the support 801 by fixing the pressure regulating valve 811 and the throttle valve 812 on the support 801.

[0067] When filtering, the handling arm 3 drives the handling tool 4 to place the receiving bottle 814 on the receiving bottle positioning rack 804, places the filter assembly 815 such as a funnel with a filter screen on the filter assembly support 803, the filter assembly 815 is connected with the receiving bottle 814, pours the sample liquid to be filtered into the funnel, the lifting assembly 807 drives the sealing pressure plate 809 to descend, the sealing pressure plate 809 is sealingly connected with the funnel, the air source is turned on, the sealing pressure plate 809 is connected with the air source and the funnel through the air inlet connector 816, and air pressure is supplied to the funnel, and the sample liquid in the funnel is filtered through air pressure.

[0068] Optionally, a buffer rubber block 817 and a liquid leakage prevention tray 818 can also be arranged below the receiving bottle positioning rack 804, the buffer rubber block 817 is used for fixing and protecting the receiving bottle 814, and the liquid leakage prevention tray 818 is used for containing the spilled liquid when pouring the sample liquid, so as to avoid liquid pollution of the workbench surface.

[0069] In some embodiments, the filtration modules 7 and 8 are arranged side by side near the second side edge, i.e. the rear side edge 112 of the workbench 11, and they can also be arranged near the third side edge, i.e. the left side edge 112 of the workbench 11. As long as they are arranged around the handling mechanism 20, it is convenient for the handling mechanism to transfer containers and / or trays from the interactive unit or the storage rack to the next process, and their positions can be arbitrarily set.

[0070] The high-pressure preparation sample in-out module 5 is arranged at the approximate center of the workbench 11 and close to the first side edge, i.e., the front side edge 111, and is adjacent to the carrying mechanism 20 to facilitate the carrying mechanism 20 to transfer samples. In some embodiments, two high-pressure preparation sample in-out modules 5 are arranged from the first side edge 111 to the second side edge 112, and the two high-pressure preparation sample in-out modules 5 are staggered in the direction along the first side edge 111, and a storage rack 2 is arranged between the high-pressure preparation sample in-out module 5 closer to the fourth side edge 114 and the carrying mechanism 20. Since the samples filtered in the filtration modules 7 and 8 need to be transferred to the high-pressure preparation sample in-out module 5 during sample processing, and the samples processed in the high-pressure preparation sample in-out module 5 need to be transferred to the pipetting module 9 to be introduced later, the storage rack 2 is arranged between the high-pressure preparation sample in-out module 5 and the carrying mechanism 20, which is more convenient for storing sample containers or trays when transferring samples between these modules. Of course, the positions of the high-pressure preparation sample in-out module 5 and the storage rack 2 can be adjusted according to actual processing needs.

[0071] Figure 7 is a perspective view of a high-pressure preparation sample in-out module according to an embodiment of the present disclosure, Figure 8 is a perspective view of a fraction collection pulling unit and a fraction collection lifting unit in a high-pressure preparation sample in-out module according to an embodiment of the present disclosure, Figure 9 is a side view of a fraction collection pulling unit and a fraction collection lifting unit in a high-pressure preparation sample in-out module according to an embodiment of the present disclosure.

[0072] The high-pressure preparation sample in-out module 5 includes a fraction collection unit 501, a fraction collection pulling unit 502, and a fraction collection lifting unit 503. The fraction collection unit 501 includes a base 514, a side wall 515 vertically arranged upwardly extending from the base 514, and a top cover 516 connected with the side wall 515, and the base 514, the side wall 515, and the top cover 516 enclose a concave space as a fraction collection operation space. The fraction collection unit 501 is provided with an unillustrated sample inlet needle and a sample outlet needle, the sample inlet needle is used to guide the sample liquid to be subjected to fraction collection, and the sample outlet needle is used to guide the sample liquid to be subjected to fraction collection to a corresponding fraction collection container after the fraction collection is completed. Correspondingly, a sample inlet position 511 is arranged in the fraction collection pulling unit 502 corresponding to the sample inlet needle of the fraction collection unit 501, which is used to place a test tube or a solvent bottle containing sample liquid to be processed, and a fraction collection position 512 is arranged in the fraction collection pulling unit 502 corresponding to the sample outlet needle of the fraction collection unit 501, which is used to place a test tube or a solvent bottle for collecting fractions. The sample inlet needle of the fraction collection unit 501 is liftable, so the sample inlet position 511 of the fraction collection pulling unit 502 can be fixed, and the sample outlet needle of the fraction collection unit 501 is fixed, so the fraction collection position 512 of the fraction collection pulling unit 502 needs to be liftable to be able to be connected with the sample outlet needle of the fraction collection unit 501.

[0073] As Figure 7 shown, the fraction collection position 512 of the fraction collection pulling unit 502 is located in the space surrounded by the base 514, the side wall 515 and the top cover 516 of the fraction collection unit 501, and a test tube and a solvent bottle are placed in the fraction collection position 512. After the fraction collection process is completed, the fraction collection position 512 can be pulled out by the carrying mechanism 20, and the test tube and the solvent bottle can be taken away. Then, the fraction collection position 512 can be pushed back. The fraction collection lifting unit 503 is located outside the space surrounded by the base 514, the side wall 515 and the top cover 516 of the fraction collection unit 501.

[0074] Referring to Figure 8 and Figure 9 , the fraction collection pulling unit 502 includes the sample injection position 511 and the fraction collection position 512. The first sliding rail 504 connected to the sample injection position 511 ensures that the sample injection position 511 can be smoothly pulled out. As Figure 8 shown, a support plate is additionally provided beside the sample injection position 511, and the first sliding rail 504 is arranged on the support plate. The second sliding rail 505 corresponding to the fraction collection position 512 is fixed on the first bottom plate 507. By arranging the hook claw on the carrying tool 4, the fraction collection position 512 can be hooked and pulled out or pushed back along the second sliding rail 505. Limiting blocks 517 are respectively arranged on the first bottom plate 507 at positions corresponding to the ends of the second sliding rail 505, and limiting grooves 518 are respectively arranged on the fraction collection position 512. When the fraction collection position 512 is pulled out along the second sliding rail 505, the limiting blocks 517 and the limiting grooves 518 can be engaged to temporarily fix the fraction collection position 512 on the second sliding rail 505, so as to facilitate the carrying mechanism 20 to place or take away the container. After the operation is completed, the fraction collection position 512 can be moved along the second sliding rail 505 by applying force to the pulling plate 506 to disengage the limiting blocks 517 and the limiting grooves 518.

[0075] A distillate collection lifting unit 503 is arranged below the first bottom plate 507 on which the second sliding rail 505 is fixed. A plurality of support rods 508 support a second bottom plate 510, and a driving motor 513 is arranged below the second bottom plate 510. A lifting rod 509 is connected to the first bottom plate 507, and the driving motor 513 can drive the lifting rod 509 to move up and down, thereby driving the first bottom plate 507 to move up and down. The distillate collection site 512 and the sample loading site 511 are provided with a protruding piece and a clamping hole, respectively. When the distillate collection site 512 is lifted, the protruding piece is separated from the clamping hole, and the distillate collection site 512 and the sample loading site 511 are separated. When the distillate collection site 512 is lowered, the protruding piece is inserted into the clamping hole, and the distillate collection site 512 and the sample loading site 511 are connected. When the distillate collection site 512 and the sample loading site 511 are connected, they can be pulled together. Here, the protruding piece can be a pin, and the clamping hole can be a pin hole, or other components that can cooperate with each other can be arranged, as long as they can be clamped and separated from each other, which is not limited here. When pulling, the clamping jaws assembled by the conveying mechanism 20 hook the pulling plate 506, so that the distillate collection site 512 and the sample loading site 511 move together.

[0076] In some embodiments, the high-pressure preparation sample loading and unloading module 5 can be compatible with multiple different container trays and can place different containers for collecting distillates condensed at different time periods or at different temperatures for subsequent analysis or use. In some embodiments, a set of sample loading sites and three sets of distillate collection sites are arranged in the high-pressure preparation sample loading and unloading module 5. The sample loading sites are used to place container trays containing samples for sample loading, and the distillate collection sites are used to place empty bottle trays for distillate collection.

[0077] In the past high-pressure preparation equipment, the sample and the product after distillate collection need to be manually taken and placed, and for different types of sample containers, such as test tubes or well plates of different specifications, the corresponding tray needs to be selected and operated and set on the software. The high-pressure preparation sample loading and unloading module of the embodiments of the present disclosure can meet the automatic operation of different types of samples, can be grabbed and pulled by the conveying mechanism, and can realize the automatic loading and unloading of the sample and the product after distillate collection. At the same time, the module itself can be lifted and lowered and can be connected to the control system of the equipment, and can automatically adapt and switch different types of containers, thereby improving the efficiency and avoiding the risks caused by manual error operation and setting.

[0078] Reference Figure 1 and 2 A pump valve analysis module 6 is arranged between the high-pressure preparation sample loading and unloading module 5 and the fourth side edge 114 of the workbench 11. In some embodiments, two pump valve analysis modules 6 are arranged corresponding to the two high-pressure preparation sample loading and unloading modules 5. The pump valve analysis module 6 is connected to the high-pressure preparation sample loading and unloading module 5 through a pipeline not shown. The pump valve analysis module 6 performs related processing of pumping and analysis.

[0079] A flow phase buffer module 10 is arranged near the fourth side edge 114 of the workbench 11, as shown in Figure 1 、 Figure 2 and Figure 13 The flow phase buffer module 10 can be a separate part from the workbench 11, and has a height lower than that of the workbench 11 so as not to affect the operation on the workbench 11. The bottom of the flow phase buffer module 10 is provided with a plurality of rollers 1003, which can be unidirectional or universal rollers and can roll on the ground to facilitate the movement of the flow phase buffer module 10. The flow phase buffer module 10 is used to buffer the flow phase required in the experiment process.

[0080] The pump valve analysis module 6 and the flow phase buffer module 10 are also connected by a pipeline not shown. The pump valve analysis module 6 introduces the sample in the high-pressure preparation sample introduction and removal module 5 into the sample through the pipeline, that is, a group of samples in the high-pressure preparation sample introduction and removal module 5, and then introduces the predetermined flow phase in the flow phase buffer module 10 according to the introduced sample, and the sample and the predetermined flow phase are mixed. The pump valve analysis module 6 separates the components of the mixed liquid and analyzes each component, and then introduces different components to the high-pressure preparation sample introduction and removal module 5 through the pipeline for fraction collection, that is, a plurality of groups of fraction collection in the high-pressure preparation sample introduction and removal module 5.

[0081] Reference Figure 1 and Figure 2 A pipetting module 9 is also arranged near the second side edge, that is, the rear side edge 112 of the workbench 11, which is used for pipetting and transferring. When a batch of high-pressure fraction collection in the high-pressure preparation sample introduction and removal module 5 is completed, the carrying mechanism 20 cooperates with the carrying tool 4 to pull out the fraction collection position of the high-pressure preparation sample introduction and removal module 5, and transfers the test tube tray collecting the fraction to the pipetting module 9. The pipetting module 9 sucks the sample from the fraction test tube and transfers the sample to the liquid phase vial for use in the next process.

[0082] Figure 10 is a perspective view of the pipetting module 9 of an embodiment of the present disclosure. The pipetting module 9 includes a bracket 901, a pipetting assembly 904 and a tip ejecting assembly 905. The bracket 901 serves as a structural support base, and the pipetting assembly 904 and the tip ejecting assembly 905 are both mounted on the bracket 901. The pipetting assembly 904 is used to connect with the suction tip 906 and control the suction tip 906 to perform liquid suction and liquid discharge operations, and the tip ejecting assembly 905 is used to separate the suction tip 906 from the pipetting assembly 904 to realize tip ejecting operation.

[0083] The support 901 comprises a support vertical plate 902 and a support horizontal plate 903 connected with each other. The support vertical plate 902 can be provided as a flat plate extending in the vertical direction, and the support horizontal plate 903 can be provided as a flat plate extending in the horizontal direction. The pipetting module 9 can further comprise a housing connected with the support vertical plate 902 and the support horizontal plate 903. The housing can jointly with the support vertical plate 902 and the support horizontal plate 903 to enclose and shield at least part of the pipetting assembly 904, the tip retracting assembly 905, etc., so as to reduce the exposed part as much as possible, on the one hand, to make the pipetting module 9 have a complete and unified appearance, and on the other hand, to play a role in dustproof and waterproof, etc.

[0084] The pipetting assembly 904 can be provided integrally on the support vertical plate 902, or can be provided mostly on the support vertical plate 902, and the rest is provided on the support horizontal plate 903. The pipetting assembly 904 is used to detachably connect with the tip 906, and is further used to drive the tip 906 to suck or spit liquid. The handling mechanism 20 can drive the support 901 and the pipetting assembly 904 and the tip retracting assembly 905 thereon to move integrally, wherein the pipetting assembly 904 can be connected with the tip 906 after moving. The specific structure of the pipetting assembly 904 can not be limited, which can provide positive pressure or negative pressure. By applying negative pressure to the tip 906, the tip 906 can suck the sample liquid to realize the liquid sucking operation, and by applying positive pressure to the tip 906, the sample liquid in the tip 906 can be pushed out to realize the liquid spitting operation.

[0085] The tip retracting assembly 905 is provided on the support horizontal plate 903, and is used to push the tip 906 to separate from the pipetting assembly 904. The specific structure of the tip retracting assembly 905 is not limited, which can move to make the tip 906 separate from the pipetting assembly 905 to realize the tip retracting operation.

[0086] The support 901 is further provided with a quick change connector 907, which can be provided on the support horizontal plate 903, for example, and is used to detachably connect with the handling mechanism 20. The specific structure of the quick change connector 907 is not limited, and the detachable connection mode includes but is not limited to pneumatic connection, magnetic connection, etc. Through the quick change connector 907, the quick connection and disconnection with the quick change connector on the handling mechanism 20 can be realized, and the automation integration degree is high.

[0087] When pipetting is performed, the carrying mechanism 20 drives the pipetting module 9 as a whole to move to the placement position of the pipette tip 906, so that the pipetting assembly 904 is connected with the pipette tip 906 at the placement position of the pipette tip 906, for example, the carrying mechanism 20 drives the pipetting assembly 904 to downwardly pierce the pipette tip 906 with a certain force, so that the pipetting assembly 904 is connected with the pipette tip 906 in interference fit, and then the pipetting module 9 and the pipette tip 906 are moved to the initial position, the pipetting assembly 904 drives the pipette tip 906 to suck liquid, and then the carrying mechanism 20 drives the pipetting module 9 and the pipette tip 906 to move from the initial position to the target position, and the pipetting assembly 904 drives the pipette tip 906 to spit liquid at the target position. Thus, one pipette tip 906 can complete the steps of installation and liquid sucking and spitting, and can perform multiple liquid sucking and spitting operations until the required volume of pipetting of one sample liquid is completed. Then, if pipetting of another sample liquid is required, the pipette tip 906 needs to be replaced, at which time the carrying mechanism 20 drives the pipetting module 9 and the pipette tip 906 to move to the recycling position, and the tip ejecting assembly 905 moves to push the pipette tip 906 connected with the pipetting assembly 904 out, so as to realize the tip ejecting operation. Then, the aforementioned installation of the pipetting assembly 904 and the pipette tip 906 and the liquid sucking and spitting operation are repeated.

[0088] Reference Figure 1 And Figure 2 The interaction unit 1, the storage rack 2, the high-pressure preparation sample in-out module 5, the positive pressure filtration module 7, the filter screen filtration module 8, and the pipetting module 9 are arranged around the carrying mechanism 20. Since these modules need to interact with the carrying mechanism 20, they need to be arranged within the operating range of the carrying mechanism 20. Modules that do not need to interact with the carrying mechanism 20, such as the pump valve analysis module, can be arranged away from the carrying mechanism 20. Through such a position arrangement, the efficiency of automatic operation can be ensured, and reasonable layout can be achieved to save space.

[0089] The mobile phase buffer module 10 is used to buffer large-capacity mobile phase liquid, which can include a plurality of mobile phase buffer tanks, Figure 11 is a perspective view of a mobile phase buffer tank. As Figure 11As shown, the box shell of the mobile phase buffer tank is provided with a turnover door 1001, and a large-capacity barrel containing mobile phase is placed inside. When the liquid in the barrel is insufficient, the turnover door 1001 is manually opened to take out the barrel to add liquid. The barrel inside communicates with the pump valve module 6 through the through hole 1002 on the box shell through a pipeline. In the prior art, the structure of the mobile phase buffer module is small in capacity, and manual liquid addition is required frequently. The bottom of the mobile phase buffer tank is provided with a plurality of rollers 1003, which can be one-way or universal rollers and can roll on the ground to facilitate the movement of the mobile phase buffer module 10. The mobile phases stored in different buffer tanks can be different. The mobile phase buffer module 10 can include any number of buffer tanks, so it can store a large amount of mobile phase liquid to ensure that the required mobile phase liquid can be continuously supplied without interruption during long-time experiments such as 24 hours, 48 hours, etc., without affecting the experimental process due to interruption for liquid supplement. The number of buffer tanks in the mobile phase buffer module 10 is not limited, as long as it can meet the experimental requirements.

[0090] Figure 12 is a front view of the automatic filtering and high-pressure separation and purification equipment with a cover body according to an embodiment of the present disclosure, Figure 13 is a back view of the automatic filtering and high-pressure separation and purification equipment with a cover body according to an embodiment of the present disclosure. Here, the front side refers to the side close to the user in the natural use state, that is, the front side of the workbench 11, and the side where the interaction unit 1 is arranged. The back side refers to the side opposite to the front side.

[0091] In some embodiments, the automatic filtering and high-pressure separation and purification equipment 100 can include a cover body 200 arranged on the workbench 11, and an internal accommodation space is formed between the cover body 200 and the workbench 11, and various processing modules introduced above are located in the accommodation space. Optionally, the cover body 200 includes a frame 600 arranged on the workbench 11, and the workbench 11 and the frame 600 can be a detachable connection structure or an integral structure, which is not limited here. In some embodiments, only the workbench 11 can be arranged without the frame 600.

[0092] The internal accommodation space is defined by the frame structure of the workbench 11 and the frame 600, that is, the upper surface of the workbench 11 and the enclosure of the frame 600 form the internal accommodation space. Optionally, the inside of the workbench 11 also has an accommodation space. In the case of multiple sub-frames of the cover body 200, each sub-frame can have its own sub-space, and among the multiple sub-spaces, some sub-spaces can be interconnected, and some sub-spaces can be relatively independent and not connected with other sub-spaces, which is not limited here.

[0093] The frames 600 are connected by plate-shaped structures to form the housing 200. The material of the housing 200 can be steel, aluminum alloy, etc., which is not limited herein. The overall shape of the housing 200 can be a cuboid or any other feasible shape, which is not limited herein. The housing 200 can include a plurality of support vertical beams, support horizontal beams, and the like to form a frame structure.

[0094] The interactive windows 300 and 301 are provided on the front side of the housing 200 and communicate the internal accommodation space and the external space. Figure 12 The illustrated interactive window 300 is in an open state, and the interactive window 301 is in a closed state. In the open state of the interactive window 300, the interactive unit 1 can interact with the outside. The mobile device moves to the interactive window 300, places the material container and / or tray delivered to the interactive unit 1, or takes away the sample container and / or tray placed on the interactive unit 1 after processing. Since the interactive window 300 is mainly used for interaction between the internal accommodation space and the external space, in order to facilitate interaction, the interactive window 300 is provided in the form of a vertically movable sliding window, which can be temporarily fixed when moved upward to the open state, keeping the window open and not hindering the interaction between the internal accommodation space and the external space.

[0095] In Figure 12 The maintenance window 400 is provided on the right side of the housing 200, i.e., the right side of the workbench 11, which is mainly used for the worker to maintain the modules in the internal accommodation space. In order to facilitate the worker to operate, the maintenance window 400 is provided in the form of a casement window, so that more internal accommodation space can be exposed when the maintenance window 400 is opened, facilitating the worker to maintain. Figure 13 On the left side and the back side of the housing of the automated filtration and high-pressure separation and purification device 100, casement window type maintenance windows 401, 402, and 403 are also provided, which can be opened by the worker to maintain the modules in the internal accommodation space.

[0096] A duct assembly 500 is further provided on the top of the housing 200 to control the working temperature of the internal accommodation space and replace the air.

[0097] Optionally, in one embodiment, the present application further provides an automated separation and purification preparation system, which includes a mobile device and at least one automated filtration and high-pressure separation and purification device 100. The mobile device is used to deliver materials to the automated filtration and high-pressure separation and purification device 100 and take away samples processed by the automated filtration and high-pressure separation and purification device 100.

[0098] For example, in the preparation stage of the experiment, the mobile device transfers various materials required for the experiment, such as a tray containing empty test tubes, a filter head tray, a Tip head tray, an empty liquid phase vial tray, etc., to the interaction unit 1 of the automated filtration and high-pressure separation and purification device 100; the sample liquid filtered by the first filtration module 7 or the second filtration module 8 can be transferred to the interaction unit 1 by the carrying mechanism 20, and then transported to other processes by the mobile device; the sample liquid subjected to fraction collection by the high-pressure preparation sample in-out module 5 and then subjected to pipetting by the pipetting module 9 can be transferred to the interaction unit 1 by the carrying mechanism 20, and then transported to other processes by the mobile device. The mobile device can be any mobile device capable of achieving the above functions in the prior art, such as but not limited to a mobile AGV trolley or a mobile robot, etc.

[0099] Example embodiments have been disclosed herein and, although the use of specific terms is expressly used herein, they are intended in the sense only of generically describing and only to the extent that is illustrative of the present disclosure. In some instances, it will be apparent to those skilled in the art that features, characteristics, and / or elements described in connection with a particular embodiment can be used in conjunction with other embodiments unless otherwise explicitly stated. As such, those skilled in the art will appreciate that various changes can be made in form and detail without departing from the scope of the disclosure as set forth in the appended claims.

Claims

1. An automated filtration and high pressure separation purification apparatus, characterized by, Comprise: a workbench; a first filter module, a second filter module, a high-pressure preparation sample in-out module, a pump valve analysis module and a carrying mechanism arranged on the workbench; and a mobile phase buffer module arranged independently of the workbench, the first filter module filters sample liquid according to first filter requirements; the second filter module filters sample liquid according to second filter requirements; the high-pressure preparation sample in-out module imports sample liquid filtered by the first filter module or the second filter module, performs fraction collection, and enables the sample liquid and the product after fraction collection to be automatically imported and exported through the carrying mechanism; the carrying mechanism is used for transferring materials in the first filter module, the second filter module and the high-pressure preparation sample in-out module; the mobile phase buffer module is used for buffering mobile phase; and the pump valve analysis module is connected with the high-pressure preparation sample in-out module and the mobile phase buffer module respectively to perform pump liquid and analysis processing.

2. The automated filtration and high pressure separation and purification apparatus of claim 1, wherein, The automatic filtration and high-pressure separation and purification equipment further comprises: an interactive unit for material interaction with the outside through the carrying mechanism; and a pipetting module for transferring the sample liquid after fraction collection to a predetermined container according to a predetermined pipetting capacity.

3. The automatic filtration and high-pressure separation and purification equipment according to claim 1, wherein the pump valve analysis module imports the sample liquid from the high-pressure preparation sample in-out module, imports the mobile phase corresponding to the sample liquid from the mobile phase buffer module, performs component separation and analysis on the mixed liquid of the sample liquid and the mobile phase, and then exports different components to the high-pressure preparation sample in-out module.

4. The automated filtration and high pressure separation and purification apparatus of claim 2, wherein, The automatic filtration and high-pressure separation and purification equipment further comprises a storage rack for placing materials required during processing in each module, the interactive unit, the storage rack, the first filter module, the second filter module, the high-pressure preparation sample in-out module and the pipetting module are arranged around the carrying mechanism and within the operating range of the carrying mechanism.

5. The automatic filtration and high-pressure separation and purification equipment according to claim 1, wherein the mobile phase buffer module comprises a plurality of mobile phase buffer boxes in which large-capacity barrels containing mobile phase are placed.

6. The automatic filtration and high-pressure separation and purification equipment according to claim 4, wherein the workbench comprises a front side, a rear side, a left side and a right side, the interactive unit is arranged on the front side, the storage rack is arranged on the left side, the first filter module, the second filter module and the pipetting module are arranged side by side on the rear side, the high-pressure preparation sample in-out module is arranged between the front side and the rear side closer to the front side, the pump valve analysis module is arranged adjacent to the high-pressure preparation sample in-out module and away from the carrying mechanism.

7. The automatic filtration and high-pressure separation and purification equipment according to claim 1, wherein the first filter module is a positive pressure filter module for filtering a small amount of sample liquid, The second filter module is a filter screen filter module, which is used for filtering a large amount of sample liquid.

8. The automatic filtration and high-pressure separation and purification device according to claim 7, wherein the positive pressure filter module comprises: a clamping mechanism for clamping a filter container containing a sample to be filtered; an air inlet connector located above the clamping mechanism and connected to an air source, for communicating the air source with the sample to be filtered during filtration; a first lifting mechanism connected to the air inlet connector, for driving the air inlet connector to lift; a second lifting mechanism, the clamping mechanism and the first lifting mechanism being fixed on the second lifting mechanism, the second lifting mechanism being used to drive the clamping mechanism, the air inlet connector and the first lifting mechanism to lift together; a pulling mechanism for pulling a plug below the filter container; and a receiving bottle placing mechanism for placing a receiving bottle for receiving filtered sample liquid.

9. The automatic filtration and high-pressure separation and purification device according to claim 8, wherein the positive pressure filter module further comprises: a base, the pulling mechanism and the receiving bottle placing mechanism being arranged on the base; and a support vertically arranged on the base, a first support plate and a second support plate being arranged on the support, the first support plate and the second support plate being arranged perpendicularly, the second support plate being fixed on the first support plate and being movable along the first support plate, the second lifting mechanism being fixedly connected to the second support plate.

10. The automatic filtration and high-pressure separation and purification device according to claim 7, wherein the filter screen filter module comprises: a vertically arranged support; a receiving bottle positioning rack arranged at the bottom of the support, for placing a receiving bottle for receiving filtered sample liquid; a filter assembly support rack arranged above the receiving bottle positioning rack and fixed on the support, for placing a filter assembly with a filter screen; a lifting mechanism arranged on the support; and a sealing pressure plate connected to the lifting mechanism, the lifting mechanism driving the sealing pressure plate to lift, an air inlet connector being arranged on the sealing pressure plate, the air inlet connector being connected to an air source, for communicating the air source with the filter assembly during filtration.

11. The automatic filtration and high-pressure separation and purification device according to claim 10, wherein the lifting mechanism comprises a lifting assembly, a linear guide rail and a motor, the linear guide rail being fixed at an upper position of the support, the lifting assembly being connected to the sealing pressure plate and being driven by the motor, the lifting assembly moving up and down along the linear guide rail.

12. The automatic filtration and high-pressure separation and purification device according to claim 1, wherein the high-pressure preparation sample inlet and outlet module comprises: a fraction collection unit comprising a sample inlet needle and a sample outlet needle, for collecting fractions of an introduced sample liquid; a fraction collection pulling unit comprising a sample inlet position corresponding to the sample inlet needle, a fraction collection position corresponding to the sample outlet needle, and an assembly capable of pulling or pushing the sample inlet position and the fraction collection position out of or into the fraction collection unit; and a fraction collection unit comprising a sample inlet needle and a sample outlet needle, for collecting fractions of an introduced sample liquid. ​ ​ ​ ​ A fraction collection lifting unit is configured to lift the fraction collection position.

13. The automatic filtration and high-pressure separation and purification device according to claim 12, wherein, the sample injection needle of the fraction collection unit is configured to be liftable, and the sample injection position of the fraction collection pulling unit is configured to be fixed; the sample outlet needle of the fraction collection unit is configured to be fixed, and the fraction collection position of the fraction collection pulling unit is configured to be liftable.

14. The automatic filtration and high-pressure separation and purification device according to claim 12, wherein, the fraction collection pulling unit comprises: a sample injection position configured to place a sample to be processed; a fraction collection position configured to collect fractions; a first sliding rail connected to the sample injection position, the sample injection position being movable along the first sliding rail; a bottom plate; a plurality of second sliding rails fixed to the bottom plate, the second sliding rails being provided corresponding to the fraction collection position, the fraction collection position being movable along the second sliding rails; and an end of the fraction collection position is provided with a pulling plate, and the fraction collection position is movable along the second sliding rails by applying force to the pulling plate.

15. The automatic filtration and high-pressure separation and purification device according to claim 14, wherein, a limit block is provided on the second sliding rail, and a limit groove is provided on the fraction collection position, and when the fraction collection position is pulled out, the fraction collection position is fixed to the second sliding rail by the limit block and the limit groove.

16. The automatic filtration and high-pressure separation and purification device according to claim 14, wherein, the fraction collection lifting unit is provided below the bottom plate and comprises a driving motor and a lifting rod, the lifting rod being connected to the bottom plate, and the driving motor driving the lifting rod to lift, the fraction collection position and the sample injection position are respectively provided with a protruding piece and a clamping hole, when the fraction collection position is lifted, the protruding piece is separated from the clamping hole, and the fraction collection position and the sample injection position are separated, when the fraction collection position is lowered, the protruding piece is inserted into the clamping hole, and the fraction collection position and the sample injection position are connected.

17. The automatic filtration and high-pressure separation and purification device according to claim 12, wherein, the high-pressure preparation sample injection and outlet module comprises a group of sample injection positions and three groups of fraction collection positions.

18. The automatic filtration and high-pressure separation and purification device according to claim 2, wherein, the pipetting module comprises: a support comprising a support vertical plate and a support horizontal plate connected to each other; a pipetting assembly provided on the support vertical plate, the pipetting assembly being configured to detachably connect with a suction head, and the pipetting assembly being further configured to drive the suction head to suck or spit liquid; and a head removal assembly provided on the support horizontal plate, the head removal assembly being configured to push the suction head to separate from the pipetting assembly.

19. The automatic filtration and high-pressure separation and purification device according to claim 2, wherein, the interaction unit comprises: a bottom plate provided on a workbench; a support plate connected to both ends of the bottom plate and vertically arranged, A placing plate is connected between the support plates and used for storing material containers and / or trays. The placing plate is provided with a plurality of first storage spaces, each of which comprises a positioning member and a sensor, the positioning member being used for limiting the position of a material container and / or a tray, and the sensor being used for detecting whether a material container and / or a tray is stored in the storage space.

20. The automatic filtration and high-pressure separation and purification device according to claim 1, characterized in that The carrying mechanism comprises a carrying arm and a carrying tool, the carrying arm being a mechanical arm or a moving mechanism having at least one degree of freedom, one end of the carrying arm being fixed to the workbench, and the other end of the carrying arm being provided with the carrying tool, the carrying tool being detachably connected to the carrying arm.

21. The automatic filtration and high-pressure separation and purification device according to claim 1, characterized in that The workbench is further provided with a tool storage for storing a plurality of carrying tools, and the carrying mechanism takes and places the carrying tools from the tool storage.

22. The automatic filtration and high-pressure separation and purification device according to claim 4, characterized in that The storage rack comprises at least one placing plate and two upright support members, the placing plate being arranged between the two support members, and the placing plate is provided with a plurality of second storage spaces for storing containers and / or trays.

23. The automatic filtration and high-pressure separation and purification device according to claim 2, characterized in that The workbench is further provided with a cover, and an internal accommodation space is formed between the cover and the workbench, On one side of the workbench provided with the interaction unit, an interaction window is arranged on the cover and used for material interaction with the external space, and on the remaining sides of the cover, maintenance windows are arranged on the cover and used for maintenance of the modules in the internal accommodation space.

24. An automated isolation and purification manufacturing system, comprising: The mobile device is used for conveying materials to the automatic filtration and high-pressure separation and purification device and taking away samples processed by the automatic filtration and high-pressure separation and purification device.

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