Purification equipment for biosurfactant rhamnolipid

Abstract

The utility model relates to the technical field of rhamnolipid purification, in particular to a biosurfactant rhamnolipid purification device which comprises an extraction tank, a fixed pipe and a movable pipe are arranged in the extraction tank, a closed transparent cup is fixedly arranged on the outer side of the extraction tank, a discharge port controlled by a valve is formed in the lower portion of the transparent cup, and a discharge port is formed in the lower portion of the transparent cup. The top face of the transparent cup is communicated with one end of an air outlet pipe, the other end of the air outlet pipe is communicated with an air pump, the fixed pipe is sealed through a valve device, and the movable pipe is driven by a lifting mechanism to move up and down. According to the device, during primary extraction, the fixed pipe and the movable block are used for synchronous suction, so that supernate in the extraction tank can be quickly sucked out, during secondary extraction, the movable pipe is used for movement and suction, so that liquid below the supernate can be effectively prevented from being sucked in, and the accuracy of the sucked supernate is guaranteed.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the purification technical field of rhamnolipid, and particularly relates to a purification device for biological surfactant rhamnolipid. BACKGROUND

[0002] Rhamnolipid is a biological surfactant of biological metabolic properties produced by pseudomonas or burkholderia. It is also a biological surfactant with the longest research time and the most mature application technology. It naturally exists in soil, water and plants. It belongs to a kind of glycolipid anionic surfactant.

[0003] The purification of rhamnolipid mainly includes drying, extraction, ceramic membrane filtration, alkali precipitation, nanofiltration membrane filtration and vacuum concentration. In the extraction, the dried powder is put into an extraction tank, and an organic solvent is added, and then the supernatant in the extraction tank is extracted.

[0004] In order to ensure complete extraction, an organic solvent is added again for extraction after the supernatant is extracted. At this moment, the extraction amount is relatively small, and when the supernatant is sucked out by using a large suction tube, the lower liquid is easily sucked into the upper layer at the same time, which is not conducive to the extraction of the supernatant. UTILITY MODEL CONTENT

[0005] The utility model provides a purification device for biological surfactant rhamnolipid to solve the defects in the prior art.

[0006] The utility model is implemented by the following technical solutions:

[0007] A purification device for biological surfactant rhamnolipid, comprising an extraction tank, a fixed pipe and a movable pipe are arranged in the extraction tank, the caliber of the fixed pipe is larger than that of the movable pipe, a closed transparent cup is fixedly arranged on the outer side of the extraction tank, a discharge port controlled by a valve is arranged at the lower part of the transparent cup, the fixed pipe is connected to the upper part of the transparent cup, one end of the movable pipe is connected to a spring pipe, the other end of the spring pipe is connected to the upper part of the transparent cup, one end of a gas outlet pipe is connected to the top surface of the transparent cup, the other end of the gas outlet pipe is connected to an air pump, the fixed pipe is closed by a valve device, and the movable pipe is driven to move up and down by a lifting mechanism.

[0008] When the application is used, when the first extraction, the air pump is started, the air pump is pumped, so that the negative pressure is formed in the transparent cup, the supernatant is sucked into the transparent cup through the moving pipe and the fixed pipe. When the second or third extraction, because the amount of supernatant is less, it will cause the position of the supernatant to rise, so first use the lifting mechanism to move the moving pipe upward, so that it is in the best suction position, then use the valve device to close the fixed pipe, and then start the air pump, so that the supernatant is only sucked into the transparent cup through the moving pipe. Because the diameter of the moving pipe is smaller than the diameter of the fixed pipe, the amount of the moving pipe is small, so that the liquid below the supernatant can be avoided to be sucked into the transparent cup. When the first extraction, the fixed pipe and the moving pipe can be sucked synchronously to realize fast suction. The transparent cup can observe whether the suction is the supernatant.

[0009] As preferred, the lifting mechanism comprises a slide way fixedly arranged along the inner side wall of the extraction tank, a sliding block is arranged in sliding fit on the slide way, a moving rod is fixedly arranged on the sliding block, a screw hole is vertically arranged on the sliding block, a screw rod is threadedly fitted in the screw hole, a round rod is coaxially fixedly connected to the upper end of the screw rod, a through hole is arranged on the upper part of the extraction tank, the round rod passes through the through hole and is rotatably connected with the through hole through a bearing, a driven gear is sleeved on the upper part of the round rod, a driving motor is fixedly arranged on the extraction tank, and a driving gear engaged with the driven gear is sleeved on the rotating shaft of the driving motor. The driving motor is started, the rotation of the rotating shaft of the driving motor drives the rotation of the driving gear, the rotation of the driving gear drives the rotation of the driven gear, the rotation of the driven gear drives the rotation of the round rod, and the rotation of the round rod drives the rotation of the screw rod along its axis. Because the screw rod is threadedly fitted with the screw hole on the sliding block, the rotation of the screw rod drives the sliding of the sliding block up and down, and in turn drives the moving of the moving pipe up and down. At the same time, the moving distance of the moving pipe up and down can be accurately controlled by using the screw rod transmission sliding table principle.

[0010] As preferred, the upper end of the fixed pipe is vertically communicated with a cross pipe, the cross pipe passes out of the extraction tank and is communicated with the transparent cup, the valve device comprises an electromagnetic valve arranged on the cross pipe, a pressure sensing unit is arranged on the bottom surface of the cross pipe, the pressure sensing unit is located directly below the moving pipe, the pressure sensing unit is signal connected with the controller, and the electromagnetic valve is controlled by the controller. The moving pipe moves upward and presses the pressure sensing unit, the pressure sensing unit senses the pressure signal and transmits the signal to the controller, and the controller controls the electromagnetic valve to be closed.

[0011] As preferred, the top surface of the moving rod is coaxially fixedly connected with an outer pipe, an inner rod is arranged in the outer pipe in cooperation, the inner rod slides along the outer pipe, one end of a spring is fixedly arranged in the outer pipe, the other end of the spring is fixedly connected with the inner end of the inner rod, and the outer end of the inner rod is fixedly connected with a pressing plate located below the pressure sensing unit.

[0012] As preferred, the horizontal pipe is detachably connected and communicated with the transparent cup through a flange.

[0013] The application has the advantages that: during the first extraction, the fixed pipe and the moving block are used for synchronous suction, so that the supernatant in the extraction tank can be quickly sucked out, and during the second extraction, the moving pipe is used for moving and suction, so that the liquid below the supernatant can be effectively avoided from being sucked, and the precision of sucking the supernatant is ensured. BRIEF DESCRIPTION OF DRAWINGS

[0014] In order to more clearly illustrate the technical scheme in the embodiments of the present application or the prior art, the drawings needed to be used in the embodiments or the prior art description will be briefly introduced as follows. Obviously, the drawings in the following description are some embodiments of the present application, and for those skilled in the art, other drawings can also be obtained without creative labor on the basis of these drawings.

[0015] Figure 1 is a structural schematic view of the present application;

[0016] Figure 2 is Figure 1 a I partial enlarged view of the present application.

[0017] shown in the drawings:

[0018] 1, extraction tank, 2, fixed pipe, 3, moving pipe, 4, transparent cup, 5, air pump, 6, sliding block, 7, screw rod, 8, driving motor, 9, driving gear, 10, driven gear, 11, round rod, 12, horizontal pipe, 13, electromagnetic valve, 14, pressure sensing unit, 15, outer pipe, 16, inner rod, 17, spring, 18, pressing plate. DETAILED DESCRIPTION

[0019] In order to make the purpose, technical scheme and advantages of the embodiments of the utility model clearer, the technical scheme in the embodiments of the utility model will be clearly and completely described in the following with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by the ordinary skilled in the art without creative labor belong to the protection scope of the utility model.

[0020] A kind of biological surfactant rhamnolipid purification equipment, as shown in Figure 1 And Figure 2 It includes extraction tank 1, fixed tube 2 and moving tube 3 are arranged in the extraction tank 1, the caliber of fixed tube 2 is greater than the caliber of moving tube 3, the outer side of extraction tank 1 is fixedly provided with closed transparent cup 4, the lower part of transparent cup 4 is provided with discharge port controlled by valve, fixed tube 2 is communicated on the upper part of transparent cup 4, one end of moving tube 3 is communicated with spring 17 tube, the other end of spring 17 tube is communicated on the upper part of transparent cup 4, the top surface of transparent cup 4 is communicated with one end of gas outlet pipe, the other end of gas outlet pipe is communicated with air pump 5, fixed tube 2 is closed by valve device, moving tube 3 is driven to move up and down by lifting mechanism.

[0021] The lifting mechanism includes slide way fixedly arranged along the inner side wall of extraction tank 1, sliding block 6 is arranged in sliding fit on the slide way, moving rod is fixedly arranged on sliding block 6, vertical screw hole is formed in sliding block 6, screw rod 7 is screw fitted in the screw hole, the upper end of screw rod 7 is coaxially fixedly connected with round rod 11, through hole is formed in the upper part of extraction tank 1, round rod 11 passes through the through hole and is rotationally connected with the through hole through bearing, driven gear 10 is sleeved on the upper part of round rod 11, driving motor 8 is fixedly arranged on extraction tank 1, the rotating shaft of driving motor 8 is sleeved with driving gear 9 engaged with driven gear 10.

[0022] The upper end of fixed tube 2 is vertically communicated with cross pipe 12, cross pipe 12 penetrates out of extraction tank 1 and is communicated with transparent cup 4, valve device includes electromagnetic valve 13 arranged on cross pipe 12, pressure sensing unit 14 is arranged on the bottom surface of cross pipe 12, pressure sensing unit 14 is located directly below moving tube 3, pressure sensing unit 14 is signal connected with controller, electromagnetic valve 13 is controlled by controller.

[0023] When the application is used, when the first extraction is performed, the air pump 5 is started, the air pump 5 sucks air, so that the negative pressure is formed in the transparent cup 4, the supernatant is sucked into the transparent cup 4 through the moving pipe 3 and the fixed pipe 2, and at this moment, the lower parts of the moving pipe 3 and the fixed pipe 2 are located on the same horizontal plane. When the second or third extraction is performed, because the amount of the supernatant is reduced, the position of the supernatant is raised, so the moving pipe 3 is first moved upward by the lifting mechanism to be in the best suction position. The lifting principle of the lifting mechanism is as follows: the driving motor 8 is started, the rotation of the rotating shaft of the driving motor 8 drives the rotation of the driving gear 9, the rotation of the driving gear 9 drives the rotation of the driven gear 10, the rotation of the driven gear 10 drives the rotation of the round rod 11, and the rotation of the round rod 11 drives the rotation of the screw rod 7 along the axial line. Because the screw rod 7 is threadedly connected with the screw holes in the sliding block 6, the rotation of the screw rod 7 drives the sliding block 6 to slide up and down, and further drives the moving pipe 3 to move up and down, and the distance of the moving pipe 3 moving up and down can be accurately controlled by the screw rod 7 transmission sliding table principle.

[0024] The moving pipe 3 is moved upward and presses the pressure sensing unit 14, the pressure sensing unit 14 transmits the signal to the controller after sensing the pressure signal, and the controller controls the electromagnetic valve 13 to be closed. Then the air pump 5 is started again, so that the supernatant is only sucked into the transparent cup 4 through the moving pipe 3. Because the caliber of the moving pipe 3 is smaller than that of the fixed pipe 2, the amount of the supernatant sucked into the transparent cup 4 through the moving pipe 3 is small, so that the liquid below the supernatant can be avoided to be sucked into the transparent cup 4. When the first extraction is performed, the supernatant can be sucked into the transparent cup 4 through the fixed pipe 2 and the moving pipe 3 synchronously, so that the supernatant can be quickly sucked. The transparent cup 4 can be used to observe whether the supernatant is sucked.

[0025] The top surface of the moving rod is coaxially and fixedly connected with the outer pipe 15, the inner rod 16 is arranged in the outer pipe 15 in a matched mode, the inner rod 16 slides along the outer pipe 15, one end of the spring 17 is fixedly arranged in the outer pipe 15, the other end of the spring 17 is fixedly connected with the inner end of the inner rod 16, and the outer end of the inner rod 16 is fixedly connected with the pressing plate 18 located below the pressure sensing unit 14. When the pressing plate 18 contacts with the pressure sensing unit 14, the inner rod 16 will press the spring 17 when the moving rod continues to rise, so that the moving pipe 3 can be lifted, and further ensure that the moving pipe 3 can move a certain distance before the pressure sensing unit 14 is pressed, so that the water suction is more accurate, and the inner rod 16 and the outer pipe 15 are matched, so that the pressing plate 18 can be opposite to the pressure sensing unit 14 when the pressing plate 18 moves.

[0026] The horizontal pipe 12 is detachably connected and communicated with the transparent cup 4 through the flange. The detachable flange can facilitate the cleaning of the transparent cup 4.

[0027] The use of the present application can quickly suck the supernatant in the extraction tank 1 by using the fixed pipe 2 and the moving block to synchronize suction at the first extraction, and can effectively avoid sucking the liquid below the supernatant by using the moving pipe 3 to move and suck at the second extraction, thereby ensuring the precision of sucking the supernatant.

[0028] Finally, it should be noted that: the above examples are only used to illustrate the technical solutions of the present application, but not to limit them; although the present application has been described in detail with reference to the foregoing examples, those skilled in the art should understand that: it can still modify the technical solutions recorded in the foregoing examples, or make equivalent replacement for part of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A purification apparatus for a biosurfactant, rhamnolipid, characterized by: The utility model provides an extraction tank, fixed pipe and moving pipe are arranged in the extraction tank, the caliber of fixed pipe is larger than the caliber of moving pipe, the outside of extraction tank is fixedly provided with closed transparent cup, the lower part of transparent cup is provided with discharge port controlled by valve, fixed pipe is communicated on the upper part of transparent cup, one end of spring pipe is communicated with moving pipe, the other end of spring pipe is communicated on the upper part of transparent cup, the top surface of transparent cup is communicated with one end of air outlet pipe, the other end of air outlet pipe is communicated with air pump, fixed pipe is closed through valve device, moving pipe is driven to move up and down through lifting mechanism.

2. The purification apparatus of biosurfactant rhamnolipid according to claim 1, characterized in that: The lifting mechanism includes a slide channel fixed along the inner side wall of the extraction tank, a sliding block is provided on the slide channel, a moving rod is fixed on the sliding block, a screw hole is vertically provided in the sliding block, a screw rod is threadedly connected in the screw hole, a circular rod is coaxially fixed on the upper end of the screw rod, a through hole is provided in the upper part of the extraction tank, the circular rod passes through the through hole and is rotatably connected to the through hole through a bearing, a driven gear is sleeved on the upper part of the circular rod, a drive motor is fixed on the extraction tank, and a driving gear engaged with the driven gear is sleeved on the rotating shaft of the drive motor.

3. The purifying apparatus of biosurfactant rhamnolipid according to claim 1, characterized in that: A horizontal pipe is vertically communicated with the upper end of the fixed pipe, the horizontal pipe passes through the extraction tank and is communicated with the transparent cup, the valve device includes an electromagnetic valve arranged on the horizontal pipe, a pressure sensing unit is arranged on the bottom surface of the horizontal pipe, the pressure sensing unit is located directly below the moving pipe, the pressure sensing unit is signal connected to a controller, and the electromagnetic valve is controlled by the controller.

4. The purification apparatus of biosurfactant rhamnolipid according to claim 3, characterized in that: The top surface of the moving rod is coaxially fixedly connected with an outer tube, an inner rod is provided in the outer tube, the inner rod slides in the outer tube, one end of a spring is fixed in the outer tube, the other end of the spring is fixedly connected to the inner end of the inner rod, and a pressing plate located below the pressure sensing unit is fixedly connected to the outer end of the inner rod.

5. The bio-surfactant rhamnolipid purification apparatus of claim 1, wherein: The horizontal pipe and the transparent cup are detachably connected and communicated through a flange.

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