Filtering device for protein purification and separation

Abstract

The utility model discloses a filtering device for protein purification and separation, which comprises a filtering component, a filtering component, a filtering component and a filtering component, and the filtering component comprises a fixed frame, a movable frame and a filter; the auxiliary clamping assembly comprises a connecting hole, a round rod, a group of limiting holes, a fixing block and a limiting rod; the connecting holes are formed in the two sides of the fixed frame and the movable frame, the round rod penetrates through the connecting holes, the limiting holes are formed in the surface of one end of the round rod, the fixed blocks are fixed above and below the middle of the outer side of the fixed frame, and the limiting rod is located between the inner sides of the fixed blocks. The auxiliary clamping assembly is arranged, the filter is placed between the fixed frame and the movable frame, the movable frame is pressed to drive the round rod to be inserted into the connecting hole in the fixed frame, then the limiting rod is inserted into the limiting hole after the round rod is inserted out, and the limiting rod is connected with the upper fixing block and the lower fixing block in a clamped mode. And therefore, the pressed movable frame and the fixed frame are limited, and the situation that the threaded rod is loosened is avoided.

Description

TECHNICAL FIELD

[0001] The utility model relates to protein purification technical field, especially a kind of filtering device for protein purification separation. BACKGROUND

[0002] Protein purification is a kind of similarity and difference between different proteins, specific protein is separated from mixture by biochemical technology, simultaneously, fish IgM monoclonal antibody also needs to be purified in processing process, and filtering device is needed for protein purification.

[0003] The existing filter is connected with the mounting frame through the fixed frame and the movable frame, so that the filter is stably placed. The fixed frame and the movable frame are located outside the filter. The outside threaded rod is rotated to press the fixed frame and the movable frame, so that the fixed frame and the movable frame clamp the filter. However, the rotating part is easy to loosen after long-term use. Therefore, an auxiliary clamping filtering device is needed to fix the fixed frame and the movable frame to the filter. SUMMARY

[0004] The utility model aims at providing a kind of filtering device for protein purification separation, to solve the problem that the existing filter is connected with the mounting frame through the fixed frame and the movable frame in the above background art, so that the filter is stably placed. The fixed frame and the movable frame are located outside the filter. The outside threaded rod is rotated to press the fixed frame and the movable frame, so that the fixed frame and the movable frame clamp the filter. However, the rotating part is easy to loosen after long-term use.

[0005] To solve the above technical problems, the utility model is realized by the following technical solutions:

[0006] The utility model is a kind of filtering device for protein purification separation, comprising:

[0007] Filtering assembly, the filtering assembly includes fixed frame, movable frame and filter;The movable frame is movably connected to one side of the fixed frame, and the filter is located inside the fixed frame and the movable frame;

[0008] Auxiliary clamping assembly, the auxiliary clamping assembly includes connecting hole, round rod, a group of limit hole, fixed block, limit rod;The connecting hole is all set up in the both sides of fixed frame and movable frame, and the round rod is penetrated between the connecting hole, the limit hole is all set up in the surface of one end of round rod, the fixed block is fixed in the middle upper and lower of fixed frame outside, the limit rod is located between the inside of fixed block, and it is penetrated limit hole.

[0009] Further, one end of the round rod is fixedly connected with a group of connecting rings, and the connecting rings are located on both sides of the movable frame.

[0010] Further, the upper fixing block is provided with a plug-in hole, and the lower fixing block is provided with a plug-in slot.

[0011] Further, the top end of the limiting rod is fixedly connected with a magnetic block, the magnetic block is clamped with the plug-in hole, and the limiting rod bottom end is plugged into the plug-in slot.

[0012] Further, the stable assembly is further included;

[0013] The stable assembly includes a magnetic plate, a connecting plate and a group of vertical rods; the magnetic plate is fixed to the bottom of one end of the fixed frame and the movable frame, the connecting plate is fixed to the upper and lower sides of the filter, and the vertical rods are all fixed to the top end of the connecting plate.

[0014] Further, the top and bottom ends of the magnetic plate are provided with a group of butt joints, and the top end of the vertical rod is plugged into the butt joint.

[0015] Further, the filter assembly further includes a mounting frame and a connecting rod; the connecting rod penetrates and is slidingly connected to the vertical surface of the mounting frame.

[0016] Compared with the prior art, the utility model has the advantages that:

[0017] One, the utility model discloses an auxiliary clamping assembly, which is arranged between the fixed frame and the movable frame, and the movable frame is pressed to make the movable frame drive the round rod to be inserted into the connecting hole in the fixed frame, then the limiting rod is inserted into the limiting hole after the round rod is pulled out, and the limiting rod is clamped with the upper and lower fixing blocks, so that the limiting rod and the fixing blocks limit the round rod after plugging, thereby limiting the pressed movable frame and the fixed frame, and the fixed frame and the movable frame clamp the filter, thereby avoiding the loosening of the threaded rod.

[0018] Two, based on the beneficial effect one, when the fixed frame and the movable frame clamp the filter, the connecting plate on the surface of the filter is attracted to the magnetic plate at the bottom of the fixed frame and the movable frame, and the vertical rod on the connecting plate is inserted into the magnetic plate, so that the magnetic plate and the connecting plate further limit the filter after clamping, thereby stabilizing the connection between the fixed frame, the movable frame and the filter. ACCURATE DRAWINGS

[0019] In order to more clearly illustrate the technical scheme of the embodiments of the utility model, the following will briefly introduce the drawings needed to be used in the embodiment description, and obviously, the drawings in the following description are only some embodiments of the utility model, and for those skilled in the art, other drawings can also be obtained according to these drawings without creating labor.

[0020] Figure 1 It is the appearance structure schematic diagram of the utility model;

[0021] Figure 2 It is auxiliary clamping assembly structure schematic view of the utility model;

[0022] Figure 3 It is auxiliary clamping assembly exploded structure schematic view of the utility model;

[0023] Figure 4 It is stable assembly structure schematic view of the utility model.

[0024] In the drawing, the component list represented by each sign is as follows:

[0025] 11, mounting frame;12, connecting rod;13, fixed frame;14, movable frame;15, filter;21, connecting hole;22, round rod;23, connecting ring;24, limiting hole;25, fixed block;26, limiting rod;27, plug-in hole;28, plug-in slot;29, magnetic block;31, magnetic plate;32, connecting plate;33, vertical rod;34, butt joint hole. Specific implementation

[0026] In order to make the above purpose, features and advantages of the utility model more obvious and easy to understand, the specific implementation of the utility model will be described in detail below with the drawings.

[0027] In the following description, a lot of specific details are set forth in order to fully understand the utility model, but the utility model can also be implemented in other ways different from the description herein, and those skilled in the art can make similar generalization without violating the connotation of the utility model, therefore the utility model is not limited by the specific implementation disclosed below.

[0028] In order to make the purpose, technical scheme and advantages of the utility model more clear, the implementation of the utility model will be further described in detail below with the drawings.

[0029] Please refer to Figures 1-3 The embodiment is a kind of filter device for protein purification separation, including:

[0030] Filter assembly, filter assembly includes fixed frame 13, movable frame 14 and filter 15;Movable frame 14 is movably connected to one side of fixed frame 13, and filter 15 is located inside fixed frame 13 and movable frame 14;

[0031] Filter assembly further includes mounting frame 11, connecting rod 12;Connecting rod 12 is connected to the vertical surface of mounting frame 11 through and slidingly;

[0032] Mounting frame 11 is used for the connection of connecting rod 12, connecting rod 12 is used for the connection of fixed frame 13, and fixed frame 13 and movable frame 14 are used for clamping filter 15 together, and filter 15 is purified and filtered by internal gel column to protein.

[0033] The auxiliary clamping assembly comprises connecting holes 21, a circular rod 22, a set of limiting holes 24, fixed blocks 25 and limiting rods 26; the connecting holes 21 are arranged on both sides of the fixed frame 13 and the movable frame 14, the circular rod 22 penetrates between the connecting holes 21, the limiting holes 24 are arranged on the surface of one end of the circular rod 22, the fixed blocks 25 are fixed to the upper and lower sides of the middle of the outer side of the fixed frame 13, the limiting rods 26 are located between the inner sides of the fixed blocks 25 and penetrate the limiting holes 24;

[0034] The connecting holes 21 are used for the insertion of the circular rod 22, the circular rod 22 is used for the arrangement of the limiting holes 24, the fixed blocks 25 are used for the limiting of the limiting rods 26 after the insertion, and the limiting rods 26 are used for the insertion into the limiting holes 24.

[0035] One end of the circular rod 22 is fixedly connected with a set of connecting rings 23, and the connecting rings 23 are located on both sides of the movable frame 14;

[0036] The upper fixed block 25 is provided with an insertion hole 27, and the lower fixed block 25 is provided with an insertion slot 28;

[0037] The top end of the limiting rod 26 is fixedly connected with a magnetic block 29, the magnetic block 29 is clamped with the inside of the insertion hole 27, and the two are adsorbed, and the bottom end of the limiting rod 26 is inserted into the insertion slot 28;

[0038] The connecting rings 23 are used for limiting the circular rod 22 during rotation, the fixed blocks 25 are used for the arrangement of the insertion hole 27 and the insertion slot 28, the insertion hole 27 is used for the insertion and adsorption of the magnetic block 29, the insertion slot 28 is used for the insertion of the bottom end of the limiting rod 26, when the limiting rod 26 is inserted, the limiting rod 26 is inserted into the insertion hole 27, and the limiting rod 26 is continuously pushed, so that the limiting rod 26 is gradually inserted into the limiting hole 24 and the insertion slot 28, when the bottom end of the limiting rod 26 is inserted into the insertion slot 28, the limiting rod 26 limits the inserted circular rod 22.

[0039] Working principle: when the assembly is not used, the fixed frame 13 and the movable frame 14 do not clamp the filter 15, when the assembly is used, the filter 15 is held by hand, the filter 15 is placed between the fixed frame 13 and the movable frame 14, the movable frame 14 is pressed by hand, so that the movable frame 14 drives the circular rod 22 to be inserted into the connecting hole 21 on the fixed frame 13, then the limiting rod 26 is held by hand, the limiting rod 26 is inserted into the insertion hole 27 from top to bottom, and the limiting rod 26 is continuously pushed, so that the limiting rod 26 is gradually inserted into the limiting hole 24 and the insertion slot 28 after the circular rod 22 is inserted, when the bottom end of the limiting rod 26 is inserted into the insertion slot 28, the limiting rod 26 limits the inserted circular rod 22, so as to limit the pressed movable frame 14 and the fixed frame 13, so that the fixed frame 13 and the movable frame 14 clamp the filter 15, thereby avoiding the loosening of the threaded rod;

[0040] The above steps can limit the fixed frame 13 and the movable frame 14 through the round rod 22 and the limiting rod 26.

[0041] Please refer to Figures 1-4 The embodiment shown is based on the above-mentioned embodiment 1, and further comprises:

[0042] The stable assembly comprises a magnetic plate 31, a connecting plate 32 and a group of vertical rods 33.

[0043] The stable assembly comprises a magnetic plate 31, a connecting plate 32 and a group of vertical rods 33.

[0044] The magnetic plate 31 is used for opening the butt joint hole 34, the connecting plate 32 is used for being adsorbed with the magnetic plate 31, and the vertical rod 33 is used for reinforcing the connection between the connecting plate 32 and the magnetic plate 31.

[0045] The magnetic plate 31 is used for opening the butt joint hole 34, the connecting plate 32 is used for being adsorbed with the magnetic plate 31, and the vertical rod 33 is used for reinforcing the connection between the connecting plate 32 and the magnetic plate 31.

[0046] The magnetic plate 31 is used for opening the butt joint hole 34, the connecting plate 32 is used for being adsorbed with the magnetic plate 31, and the vertical rod 33 is used for reinforcing the connection between the connecting plate 32 and the magnetic plate 31.

[0047] The magnetic plate 31 is used for opening the butt joint hole 34, the connecting plate 32 is used for being adsorbed with the magnetic plate 31, and the vertical rod 33 is used for reinforcing the connection between the connecting plate 32 and the magnetic plate 31.

[0048] The magnetic plate 31 is used for opening the butt joint hole 34, the connecting plate 32 is used for being adsorbed with the magnetic plate 31, and the vertical rod 33 is used for reinforcing the connection between the connecting plate 32 and the magnetic plate 31.

[0049] The magnetic plate 31 is used for opening the butt joint hole 34, the connecting plate 32 is used for being adsorbed with the magnetic plate 31, and the vertical rod 33 is used for reinforcing the connection between the connecting plate 32 and the magnetic plate 31.

[0050] Finally, it should be noted that: the above only for the preferred embodiments of the present application, and is not intended to limit the present application, although the foregoing embodiments of the present application have been described in detail, for the skilled in the art, it still can be modified, or for the equivalent replacement of part of the technical features of the technical solutions recorded in the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application, shall be included within the scope of the present application.

Claims

1. A filter device for protein purification and isolation, characterized in that Include: Filter assembly, the filter assembly includes a fixed frame (13), movable frame (14) and filter (15); The movable frame (14) is movably connected to one side of the fixed frame (13), and the filter (15) is located inside the fixed frame (13) and the movable frame (14); Auxiliary clamping assembly, the auxiliary clamping assembly includes connecting hole (21), round bar (22), a set of limiting hole (24), fixed block (25), limiting rod (26); The connecting hole (21) is opened on both sides of the fixed frame (13) and the movable frame (14), the round bar (22) is penetrated between the connecting hole (21), the limiting hole (24) is opened on the surface of one end of the round bar (22), the fixed block (25) is fixed on the outside of the fixed frame (13) between the upper and lower sides, the limiting rod (26) is located between the inside of the fixed block (25), and penetrates the limiting hole (24).

2. The filter device for protein purification and isolation according to claim 1, characterized in that, One end of the round bar (22) is fixedly connected with a group of connecting rings (23), and the connecting rings (23) are located on both sides of the movable frame (14).

3. The filter device for protein purification and separation according to claim 1, characterized in that, The upper fixed block (25) is provided with a plug hole (27), and the lower fixed block (25) is provided with a plug slot (28).

4. The filter device for protein purification and separation according to claim 1, characterized in that, The top end of the limiting rod (26) is fixedly connected with a magnetic block (29), the magnetic block (29) is connected with the inside of the plug hole (27), and the two are adsorbed, and the bottom end of the limiting rod (26) is inserted into the plug slot (28).

5. The filter device for protein purification and isolation according to claim 1, characterized in that, It also includes a stable assembly; The stable assembly includes a magnetic plate (31), a connecting plate (32) and a group of vertical rods (33); The magnetic plate (31) is fixed to the bottom of one end of the fixed frame (13) and the movable frame (14), the connecting plate (32) is fixed to the upper and lower sides of the filter (15), and the vertical rod (33) is fixed to the top end of the connecting plate (32).

6. A filter device for protein purification and isolation according to claim 5, characterized in that The top and bottom ends of the magnetic plate (31) are provided with a group of butt joints (34), and the top end of the vertical rod (33) is inserted into the inside of the butt joint (34).

7. The filter device for protein purification and isolation according to claim 1, characterized in that, The filter assembly further includes a mounting frame (11) and a connecting rod (12); The connecting rod (12) penetrates and is slidably connected to the vertical surface of the mounting frame (11).

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