Card box for nucleic acid detection

Abstract

The utility model discloses a card box for nucleic acid detection, and relates to the field of nucleic acid detection equipment, the card box comprises a card box upper split body, the card box upper split body is provided with a sample adding port, and a sample adding cover is arranged at the sample adding port; the card box lower split body is rotationally connected with the card box upper split body, and a plurality of detection grooves are formed in the card box lower split body; the sealing film is arranged at the notch of the detection groove in a sealing manner; and the pipettor is used for transferring the solution in the detection tank into other detection tanks. The lower split body of the card box is driven by an external mechanism to rotate, a sample solution in the detection groove is transferred into other detection grooves through the pipettor so as to complete nucleic acid extraction and purification and / or PCR amplification detection, the whole process is carried out in the closed card box, and aerosol pollution is effectively prevented; according to the card box, the card box upper split body and the card box lower split body form the whole card box integrated structure, the structure is simple, use is convenient, the machining cost is low, and more importantly, compared with a card box of a non-integrated structure, the leakproofness is higher.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of nucleic acid detection equipment, in particular to a nucleic acid detection cartridge. BACKGROUND

[0002] Nucleic acid detection is widely used in various fields of biomedicine, especially in the detection of infectious disease pathogens. The result of nucleic acid detection is an important criterion for diagnosis. The traditional nucleic acid detection process is complicated, including sample lysis, washing, nucleic acid elution, PCR amplification, and result detection. It needs to be operated in different instruments and different independent laboratories, which not only puts forward higher requirements for experimenters and experimental environment, but also increases the risk of cross contamination in the process of multiple transfers, thereby affecting the accuracy of the detection result.

[0003] Nucleic acid extraction and polymerase chain reaction (PCR) are common analysis methods in molecular biology. Most nucleic acid extraction and PCR are performed in separate instruments. At the same time, consumables are also separate, which is not conducive to system integration. The separate process of nucleic acid extraction and purification and PCR amplification and detection can easily lead to cross contamination, false positives of detection results, and aerosol diffusion, which pollutes the environment. SUMMARY

[0004] In order to facilitate operation and prevent contamination, the present application provides a nucleic acid detection cartridge.

[0005] The nucleic acid detection cartridge provided by the present application adopts the following technical solutions:

[0006] A nucleic acid detection cartridge, comprising:

[0007] An upper cartridge, provided with a sample addition port, and a sample addition cover is arranged at the sample addition port;

[0008] A lower cartridge, rotatably connected with the upper cartridge, and a plurality of detection grooves are integrally formed in the lower cartridge;

[0009] A sealing film, sealingly arranged at the groove opening of the detection groove;

[0010] A pipette, arranged in the upper cartridge, for transferring the solution in the detection groove to other detection grooves.

[0011] By adopting the technical scheme, a plurality of detection grooves are arranged in the lower part of the cartridge, and reagent solution and / or cleaning solution are preset in the corresponding detection grooves before the experiment; during the experiment, the sample is added into the detection groove through the sample adding opening, and the sample adding cover is closed; during the experiment, the lower part of the cartridge is driven to rotate by an external mechanism, and the sample solution in the detection groove is transferred to other detection grooves by the pipette, so as to complete the nucleic acid extraction and purification and / or PCR amplification detection, and the whole process is carried out in the cartridge, which effectively prevents pollution; and in the application, the upper part and the lower part of the cartridge constitute an integrated structure of the cartridge, which is simple in structure, convenient to use, low in processing cost, and has higher sealing performance compared with a non-integrated structure cartridge.

[0012] Optionally, the pipette comprises:

[0013] A pipette is arranged in the upper part of the cartridge along the central axis of the cartridge, and an opening is arranged at the end of the pipette away from the lower part of the cartridge.

[0014] A needle is arranged for puncturing the sealing film and inserting into the detection groove, the needle is connected to the end of the pipette close to the lower part of the cartridge, and the needle is in communication with the pipette.

[0015] A reset member is arranged in the upper part of the cartridge for driving the pipette to move away from the lower part of the cartridge, so that the needle is separated from the detection groove.

[0016] By adopting the technical scheme,

[0017] Optionally, a sealing tube is fixedly arranged in the upper part of the cartridge along the axial direction of the cartridge, the pipette is arranged in the sealing tube, and a boss is arranged on the pipette for abutting against the inner wall of the sealing tube.

[0018] An end plug is arranged at the bottom of the sealing tube, a through hole is arranged in the end plug for the needle to pass through, and the needle is in abutment with the inner wall of the through hole.

[0019] Optionally, a first filter element is arranged in the pipette.

[0020] Optionally, a second filter element is arranged in the sample adding opening.

[0021] Optionally, the upper part of the cartridge is provided with at least two positioning planes, and the positioning planes extend along the height direction of the upper part of the cartridge.

[0022] Optionally, a positioning groove is arranged on the positioning plane, and the positioning groove is arranged along the direction perpendicular to the axis of the cartridge.

[0023] Optionally, the detection groove comprises one or more of a washing groove, a lysis groove, an elution groove and a PCR groove.

[0024] In summary, the present application comprises at least one of the following beneficial technical effects:

[0025] A plurality of detection grooves are arranged in the lower part of the cartridge, and reagent solutions and / or cleaning solutions are preset in the corresponding detection grooves before the experiment. During the experiment, the lower part of the cartridge is driven to rotate by an external mechanism, and the sample solution in the detection groove is transferred to other detection grooves by a pipette to complete nucleic acid extraction and / or PCR amplification detection. The entire process is carried out in a closed cartridge, effectively preventing contamination. In the present application, the upper part of the cartridge and the lower part of the cartridge form an integrated structure of the entire cartridge, which is simple in structure, easy to use, low in processing cost, and more importantly, has stronger sealing than a non-integrated structure cartridge. BRIEF DESCRIPTION OF DRAWINGS

[0026] Figure 1 is a schematic diagram of the overall structure of the embodiment of the present application;

[0027] Figure 2 is a schematic diagram of the structure of the lower part of the cartridge of the embodiment of the present application;

[0028] Figure 3 is a schematic diagram of the structure of the lower part of the cartridge of the embodiment of the present application from another perspective;

[0029] Figure 4 is a top view of the cartridge of the embodiment of the present application;

[0030] Figure 5 is an A-A sectional view of the embodiment of the present application; Figure 4

[0031] Figure 6 is a schematic diagram of the structure of the pipette mainly embodied in the embodiment of the present application.

[0032] BRIEF DESCRIPTION OF DRAWINGS: 1, upper part of the cartridge; 11, positioning plane; 111, positioning groove; 12, sealing tube; 121, end plug; 122, limiting ring; 2, lower part of the cartridge; 21, sealing film; 3, pipette; 31, pipette tube; 311, boss; 312, first filter element; 32, needle; 33, reset member; 4, sample addition cover; 41, cover body; 411, accommodating groove; 412, second filter element; 42, cover plate; 421, air pressure balance hole; 5, detection groove; 51, washing groove; 52, lysis groove; 53, elution groove; 54, PCR groove; 55, combination groove; 56, small hole position groove. DETAILED DESCRIPTION

[0033] ​With reference to the accompanying drawings, the technical solutions in the embodiments of the present application will be clearly and completely described below. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments of the present application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of the present application.

[0034] It should be noted that all the direction indications (such as up, down, left, right, front, back, and the like) in the embodiments of the present application are only used to explain the relative position relationship, movement condition and the like between components in a certain posture (as shown in the drawings), and if the certain posture changes, the direction indications also change accordingly.

[0035] In the present application, unless specifically defined and limited otherwise, the terms "connection", "fixed", and the like should be understood in a broad sense, for example, "fixed" can be fixed connection, or detachable connection, or integral; can be mechanical connection, or electrical connection; can be direct connection, or indirect connection through an intermediate medium; can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise specifically limited. For those of ordinary skill in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0036] In addition, if the present application has descriptions involving "first", "second", and the like, the "first", "second", and the like are only for the purpose of description, and cannot be understood as indicating or implying the relative importance of the indicated technical features or implicitly indicating the number of the indicated technical features. Therefore, the features limited by "first" and "second" can explicitly or implicitly include at least one of the features. In addition, the meaning of "and / or" appearing throughout the text includes three parallel solutions. For example, "A and / or B" includes A solution, or B solution, or A and B solutions. In addition, the technical solutions of each embodiment can be combined with each other, but it must be based on the realization of those of ordinary skill in the art, and when the combination of technical solutions appears contradictory or unachievable, it should be considered that the combination of technical solutions does not exist, and is not within the scope of protection claimed by the present application.

[0037] The embodiment of the present application discloses a nucleic acid detection cartridge.

[0038] Reference Figure 1The nucleic acid detection cartridge comprises an upper cartridge body 1, a lower cartridge body 2 and a pipette 3. The lower cartridge body 2 is rotatably connected to the upper cartridge body 1. The cross section of the lower cartridge body 2 and the upper cartridge body 1 is circular to facilitate rotation. The lower cartridge body 2 and the upper cartridge body 1 are integrally formed into a shell structure. The upper cartridge body 1 and the lower cartridge body 2 are open at one end and closed at the other end to form a relatively sealed shell.

[0039] With reference to Figure 1 The upper cartridge body 1 is provided with at least two positioning planes 11 extending along the height direction of the upper cartridge body 1. During testing, the cartridge is installed in the installation cavity of the testing instrument. The installation cavity of the instrument has a positioning plate matched with the positioning plane 11. The positioning plane 11 abuts against the positioning plate to limit the position of the upper cartridge body 1 and keep the position of the upper cartridge body 1 stable during rotation of the lower cartridge body 2.

[0040] To improve the sealing performance, a sealing ring is arranged at the connection between the upper cartridge body 1 and the lower cartridge body 2. The sealing ring is an O-shaped sealing ring.

[0041] Further, with reference to Figure 1 A positioning groove 111 is arranged on the positioning plane 11 and extends along the direction perpendicular to the axis of the cartridge. The positioning groove 111 can be a V-shaped groove, a T-shaped groove or other shaped grooves. In this embodiment, the positioning groove 111 is a V-shaped groove. A positioning part is arranged on the instrument to be inserted into the positioning groove 111 to limit the vertical degree of freedom of the cartridge.

[0042] Optionally, with reference to Figure 2 and Figure 3, the detection cartridge lower part 2 is integrally formed with a plurality of detection grooves 5, the detection grooves 5 include one or more of a washing groove 51, a lysis groove 52, an elution groove 53 and a PCR groove 54. In this embodiment, the detection grooves 5 include the washing groove 51, the binding groove 55, the lysis groove 52, the elution groove 53 and the PCR groove 54, specifically, one elution groove 53, one lysis groove 52, one binding groove 55, three washing grooves 51 and a plurality of PCR grooves 54, the elution groove 53 is provided with nucleic acid eluent, the lysis groove 52 is provided with lysis solution, the binding groove 55 is reserved with incubation buffer, the washing groove 51 is provided with washing solution, and the PCR groove 54 is the position of different target PCR primer probes. The PCR groove 54 is a circular groove, and protrudes from the bottom of the detection cartridge lower part 2 to form a plurality of downward extending cylindrical structures; the washing groove 51 and the binding groove 55 are fan-shaped grooves, and protrude from the bottom of the detection cartridge lower part 2. The detection grooves 5 all protrude downward from the bottom of the detection cartridge lower part 2 to form protruding structures corresponding to the detection grooves 5, which are adapted to the driving mechanism of the detection equipment, so that the driving mechanism of the detection equipment can drive the lower part to rotate. Further, in this embodiment, the detection grooves 5 also include four small hole position grooves 56, and the four small hole position grooves 56 are respectively provided with protease K, magnetic beads, paraffin oil and PCR enzyme freeze-dried small balls.

[0043] In order to prevent pollution, a sealing film 21 (see Figure 5 ) is sealingly arranged at the groove opening of the detection groove 5. In this embodiment, the sealing film 21 is made of aluminum plastic film.

[0044] Referring to Figure 4 and Figure 5 , the detection cartridge upper part 1 is provided with a sample adding port, and the sample adding port is provided with a sample adding cover 4. The detection cartridge lower part 2 is integrally formed with a plurality of detection grooves 5; when adding a sample, the sample adding cover 4 is opened, a sample solution is sucked by a sample adding device and is inserted into the detection cartridge upper part 1 through the sample adding port, the sample solution is added into the detection grooves 5, and then the sample adding cover 4 is closed. The sample adding cover 4 includes a cover body 41 and a cover plate 42, the cover body 41 and the cover plate 42 are integrally formed, when the sample adding cover 4 is closed, the cover body 41 is inserted into the sample adding port, the cover plate 42 abuts against the top wall of the detection cartridge upper part 1, and the size of the cover plate 42 is greater than that of the sample adding port.

[0045] Referring to Figure 4 and Figure 5 , the cover body 41 is provided with a containing groove 411, and the cover plate 42 is provided with an air pressure balance hole 421, the air pressure balance hole 421 communicates with the containing groove 411, the air pressure balance hole 421 is arranged to ensure that the inside of the detection cartridge can communicate with the outside air, so that the test can be carried out normally. In order to prevent pollution, a second filter element 412 is arranged at the sample adding port, specifically, the second filter element 412 is embedded in the containing groove 411, which effectively prevents aerosol from diffusing from the air pressure balance hole 421.

[0046] Referring toFigure 4 and Figure 5 The pipette 3 is arranged in the upper part 1 of the cartridge for transferring the solution in the detection groove 5 to other detection grooves 5. The pipette 3 comprises a pipette tube 31, a needle 32 and a reset member 33.

[0047] Optionally, a sealing tube 12 is arranged in the upper part 1 of the cartridge along the axial direction of the cartridge. The top of the sealing tube 12 is open and penetrates the top wall of the upper part 1 of the cartridge. The top wall of the upper part 1 of the cartridge refers to the end face of the upper part 1 of the cartridge away from the lower part 2 of the cartridge, i.e. the uppermost wall of the upper part 1 of the cartridge during the test process. The pipette tube 31 is slidingly arranged in the upper part 1 of the cartridge along the central axis of the cartridge. The central axis of the cartridge refers to the vertical axis of the cartridge during use. The end of the pipette tube 31 away from the lower part 2 of the cartridge is open.

[0048] Referring to Figure 5 and Figure 6 The pipette tube 31 is slidingly arranged in the sealing tube 12. The pipette tube 31 is provided with a boss 311 for abutting against the inner wall of the sealing tube 12. The boss 311 is circular. In order to increase the sealing effect, the side wall of the boss 311 is adapted to the inner wall of the sealing tube 12 and abuts against the inner wall of the sealing tube 12.

[0049] Referring to Figure 5 and Figure 6 The bottom of the sealing tube 12 is provided with an end plug 121. The end plug 121 can be fixed to the sealing tube 12 by welding, bonding or threaded connection. A through hole is formed in the end plug 121 for the needle 32 to pass through. The needle 32 is connected to the end of the pipette tube 31 close to the lower part 2 of the cartridge. The needle 32 communicates with the pipette tube 31. The needle 32 is used to pierce the sealing film 21 and insert into the detection groove 5. The needle 32 abuts against the inner wall of the through hole.

[0050] Optionally, referring to Figure 5 and Figure 6 The reset member 33 is arranged in the upper part 1 of the cartridge for driving the pipette tube 31 to move away from the lower part 2 of the cartridge, so that the needle 32 is separated from the detection groove 5. In this embodiment, the reset member 33 is a reset spring. The reset spring is located in the sealing tube 12 and is sleeved on the pipette tube 31. One end of the reset spring abuts against the boss 311, and the other end abuts against the end plug 121. After the needle 32 is inserted into the detection groove 5 and the solution is sucked, the elastic force of the reset spring can drive the pipette tube 31 and the needle 32 to automatically reset upwards, so that the needle 32 is separated from the detection groove 5.

[0051] Referring to Figure 6The pipe opening of the sealing pipe 12 is provided with a limiting ring 122 for abutting against the boss 311, the limiting ring 122 is integrally formed with the upper part 1 of the cartridge, under the action of the reset spring, the pipette 31 has a tendency to move upward, when the boss 311 abuts against the limiting ring 122, at this time, the position of the pipette 31 is limited, preventing the pipette 31 from being separated from the sealing pipe 12.

[0052] With reference to Figure 6 The pipette 31 is provided with a first filter core 312, the first filter core 312 is attached to the inner wall of the pipette 31, and the first filter core 312 is located at the upper end of the pipette 31, so as to increase the effective pipetting volume of the pipette, and the effective pipetting volume is the volume of the cavity between the first filter core 312 and the bottom of the pipette 31. The first filter core 312 can allow gas to pass through and can block aerosol from passing through, effectively preventing the aerosol inside the cartridge from diffusing to the outside of the cartridge, and preventing the aerosol from being contaminated.

[0053] The implementation principle of the embodiment of the present application is that a plurality of detection grooves 5 are arranged in the lower part 2 of the cartridge, and reagent solutions and / or cleaning solutions are pre-set in the corresponding detection grooves 5 before the experiment; during the experiment, the sample is added to the detection groove 5 through the sample adding port, and the sample adding cover 4 is closed; during the experiment, the lower part 2 of the cartridge is driven to rotate by an external mechanism, and the sample solution in the detection groove 5 is transferred to other detection grooves 5 by the pipette 3, so as to complete the nucleic acid extraction and purification and / or PCR amplification detection. Specifically, the pipetting mechanism on the experimental instrument is in communication with the top of the pipette 31, the pipette 31 is driven to move downward, so that the needle 32 is inserted into the detection groove, air is sucked to form negative pressure in the pipette 31, and then the solution in the detection groove 5 is sucked through the needle 32, and then the pipetting mechanism moves upward, and under the action of the reset member, the pipette moves upward to reset, and then the lower part 2 of the cartridge is driven to rotate, when the needle 32 is opposite another corresponding detection groove 5, the solution is released, the whole process is carried out in the cartridge, which effectively prevents contamination; and in the present application, the upper part 1 of the cartridge and the lower part 2 of the cartridge constitute an integrated structure of the cartridge, which is simple in structure, convenient to use, and low in processing cost.

[0054] The above are preferred embodiments of the present application, which do not limit the protection scope of the present application, therefore: any equivalent changes made on the basis of the structure, shape, principle of the present application should be covered within the protection scope of the present application.

Claims

1. A cartridge for nucleic acid detection, characterized by, The cartridge comprises: a cartridge upper part (1) provided with a sample adding opening, wherein a sample adding cover (4) is arranged at the sample adding opening; a cartridge lower part (2) rotationally connected with the cartridge upper part (1), wherein a plurality of detection grooves (5) are integrally arranged in the cartridge lower part (2); a sealing film (21) sealingly arranged at the groove opening of the detection groove (5); a pipette (3) arranged in the cartridge upper part (1) and used for transferring a solution in the detection groove (5) to another detection groove (5).

2. The cartridge for nucleic acid detection according to claim 1, wherein The pipette (3) comprises: a pipette tube (31) slidingly arranged in the cartridge upper part (1) along the central axis of the cartridge, and an opening is arranged at one end of the pipette tube (31) away from the cartridge lower part (2); a needle (32) used for puncturing the sealing film (21) and inserted into the detection groove (5), wherein the needle (32) is connected to one end of the pipette tube (31) close to the cartridge lower part (2), and the needle (32) is in communication with the pipette tube (31); a reset member (33) arranged in the cartridge upper part (1) and used for driving the pipette tube (31) to move away from the cartridge lower part (2) so that the needle (32) is separated from the detection groove (5).

3. The cartridge for nucleic acid detection according to claim 2, wherein A sealing tube (12) is fixedly arranged in the cartridge upper part (1) along the axial direction of the cartridge, the pipette tube (31) is slidingly arranged in the sealing tube (12), and a boss (311) is arranged on the pipette tube (31) and used for abutting against the inner wall of the sealing tube (12). An end plug (121) is arranged at the bottom of the sealing tube (12), a through hole is arranged in the end plug (121) and used for allowing the needle (32) to pass through, and the needle (32) abuts against the inner wall of the through hole.

4. The cartridge for nucleic acid detection according to claim 2, wherein A first filter element (312) is arranged in the pipette tube (31).

5. The cartridge for nucleic acid detection according to claim 1, wherein A second filter element (412) is arranged at the sample adding opening.

6. The cartridge for nucleic acid detection according to claim 1, wherein The cartridge upper part (1) is provided with at least two positioning planes (11) extending along the height direction of the cartridge upper part (1).

7. The cartridge for nucleic acid detection according to claim 6, wherein A positioning groove (111) is arranged on the positioning plane (11) and arranged along the direction perpendicular to the axis of the cartridge.

8. The cartridge for nucleic acid detection according to claim 1, wherein The detection groove (5) comprises one or more of a washing groove (51), a lysis groove (52), an elution groove (53), and a PCR groove (54).

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