Stock solution RNA (Ribonucleic Acid) sample transfer heat preservation box

Abstract

The utility model discloses a stock solution RNA sample transfer heat preservation box which comprises a cylindrical box body, the upper side of the box body is an opening side, a supporting frame is arranged in the box body, the supporting frame is detachably installed and is divided into a plurality of layers of supporting structures from bottom to top, each layer of supporting structure comprises a supporting plate, and the centers of the supporting plates are fixed to the same column body. The upper side supporting face of the supporting plate is divided into a plurality of fan-shaped supporting faces through partition plates, an interlayer is arranged on the side wall of the box body, and the interlayer corresponds to the inner side wall of the box body and is in a grid shape. The box cover is used for sealing the opening side of the upper side of the box body. An annular interlayer is arranged in the kit body, cooling is carried out through ice cubes, the temperature in the inner cavity is about 0 DEG C, the supporting frame can be taken out of the kit body, reagent bottles are marked and then placed in the containing grooves in the supporting plate, then the reagent bottles are placed in the kit body, the kit body is used for keeping the low-temperature environment in the kit body in the transportation process, and the influence of temperature changes on a stock solution RNA sample is reduced.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the technical field of biological preparation equipment, and particularly relates to a stock solution RNA sample transfer incubator. BACKGROUND

[0002] The mRNA vaccine introduces mRNA encoding specific antigens into the human body, so that the human cells can transcribe these mRNA and produce corresponding antigen proteins, thereby stimulating the immune system to produce an immune response. The mRNA vaccine preparation process is roughly as follows: usually, Escherichia coli is used as a carrier, and plasmid DNA (pDNA) is obtained through fermentation, alkaline lysis and other processes. After the plasmid DNA is cut by enzymes and transcribed in vitro, mRNA is obtained, and then the product is finally prepared through lipid nanoparticle (LNP) technology. Each operation step is closely related, and all needs to involve a purification process. The upstream stage and the downstream stage are often not in the same laboratory, and there is also a time span. In order to ensure the activity of the sample, the sample to be treated needs to be preserved in an ultra-low temperature refrigerator. In the laboratory, high-value biological products such as cells, microorganisms and biological samples need to be stored in an environment of-80 degrees to ensure their integrity and stability. The transportation time is as short as possible, and the stock solution RNA sample needs to use a heat preservation box with refrigeration function.

[0003] The utility model mainly prepares a kind of stock solution RNA sample's transport heat preservation box, can be built-in cold source, ensure in short time transportation process, as far as possible guarantee sample keeps activity. CONTENT OF THE UTILITY MODEL

[0004] The utility model aims at providing stock solution RNA sample transfer incubator, can be built-in cold source, ensure in short time transportation process, as far as possible guarantee sample keeps activity.

[0005] To solve the above technical problems, the utility model is realized by the following technical scheme:

[0006] The stock solution RNA sample transfer incubator includes the box body of cylinder, and the upper side of the box body is open side, and a support frame is built-in for supporting the stock solution RNA sample reagent bottle;

[0007] The support frame can be detachably installed, and is divided into multiple layers of support structures from bottom to top, and a single layer of support structure includes a support plate, the support plate is circular, and the center is fixed to the same column, the upper side of the support plate has a placing groove for accommodating the reagent bottle, and the upper side of the support plate is divided into multiple fan-shaped support surfaces by a partition plate;

[0008] The side wall of the box body has a sandwich layer, and the sandwich layer corresponds to the grid-shaped inner side wall of the box body;

[0009] It also includes a box cover for closing the upper side opening side of the box body.

[0010] Further, the bottom of the box cavity is provided with a tray, the tray is rotatably arranged at the bottom of the box, the tray is used for placing a support frame, the tray is provided with a positioning column in the center, and the support frame is provided with a positioning hole corresponding to the lowermost support plate.

[0011] Further, the column body is a tubular structure, hollow inside, and provided with air holes in the side wall, the box cover is provided with a communication pipe at the opening side of the upper end of the column body, and the communication pipe is sleeved with the opening side of the upper end of the column body.

[0012] Further, a fan is arranged in the communication pipe, and the communication pipe is sealed by a sealing cover corresponding to the pipe opening side of the box cover.

[0013] Further, the box cover is provided with an annular groove corresponding to the edge position, and a sealing ring is arranged in the annular groove and corresponds to the opening side of the upper side of the interlayer for sealing the upper side of the interlayer.

[0014] Further, the bottom of the box body is provided with a base, the inside of the base is a water storage tank, and the bottom of the box body is provided with a water permeation hole corresponding to the bottom of the interlayer.

[0015] The utility model has the following beneficial effects: the utility model is a box structure, provided with an annular interlayer, and the temperature in the cavity is about 0 DEG C through ice block cooling, the support frame can be taken out from the box, the reagent bottle is marked and placed in the placing groove on the support plate, and then is placed in the box, used for keeping the low temperature environment in the box during transportation, and reducing the influence of temperature change on the original liquid RNA sample. BRIEF DESCRIPTION OF DRAWINGS

[0016] In order to more clearly illustrate the technical scheme of the embodiment of the utility model, the following will briefly introduce the drawings needed to be used for the embodiment description.

[0017] Figure 1 The utility model support frame takes out structure schematic diagram.

[0018] Figure 2 The utility model structure schematic diagram under closed state.

[0019] Figure 3 The utility model box cover installation and disassembly structure schematic diagram.

[0020] Figure 4 The utility model box body bottom and support frame bottom cooperation structure schematic diagram.

[0021] Figure 5 The utility model base dismounting structure schematic diagram.

[0022] The components represented by each number in the attached diagram are listed below: Box 1, Support frame 2, Support plate 21, Column 4, Placement slot 22, Partition 23, Interlayer 11, Box lid 3, Tray 5, Positioning post 51, Positioning hole 24, Air hole 41, Connecting pipe 31, Fan 32, Sealing cover 33, Annular groove 34, Sealing ring 6, Base 7, Drain hole 12. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0024] like Figures 1-2 As shown: The raw RNA sample transport and insulation box includes a cylindrical box body 1 with an open side on the top and a built-in support frame 2 for supporting the raw RNA sample reagent bottle.

[0025] The support frame 2 is detachable and can be installed in multiple layers from bottom to top. Each layer of the support structure includes a support plate 21, which is circular and fixed to the same column 4 in the center. The upper side of the support plate 21 has a placement slot 22 for accommodating reagent bottles. The upper support surface of the support plate 21 is divided into multiple fan-shaped support surfaces by partitions 23. Each partition is divided into four equal fan-shaped areas. The entire support frame is detachable and can be removed from the opening side of the box for easy access to reagent bottles.

[0026] The side wall of the box 1 has a layer 11, which is a grid-like structure corresponding to the inner side wall of the box 1. The layer is used to place a cold source, specifically crushed ice. After the layer is filled with ice, it wraps around the inner cavity of the box in a ring shape.

[0027] It also includes a lid 3 for sealing the opening on the upper side of the box body 1. This lid is used to seal the opening, isolate the external environment, and prevent heat exchange.

[0028] This invention features a box structure with an internal annular interlayer. Ice is used to cool the interior, keeping the temperature around 0°C. The support frame can be removed from the box, and the reagent bottles are marked and placed in the slots on the support plate before being put back into the box. This design maintains a low-temperature environment inside the box during transportation, reducing the impact of temperature changes on the original RNA sample.

[0029] like Figure 3As shown: the bottom of the cavity of the box body 1 is provided with a tray 5, the tray 5 is rotatably arranged at the bottom of the box body 1, the tray 5 is used for placing the support frame 2, the tray 5 is provided with a positioning column 51 in the center, and the support frame 2 is provided with a positioning hole 24 corresponding to the lowermost support plate 21. The positioning hole is located in the center of the support plate, which is used for accurately placing the whole support frame, and the tray can rotate around the axis of the positioning column, so that the positions of the reagent bottles can be adjusted, and the taking and placing operation is facilitated.

[0030] As shown in the figure: Figure 4 The column 4 is a tubular structure, hollow inside, and the side wall is provided with an air hole 41. The box cover 3 is provided with a connecting pipe 31 corresponding to the upper end opening side of the column 4, and the connecting pipe 31 and the upper end opening side of the column 4 are mutually sleeved. The connecting pipe is used for connecting the outside of the box body through the box cover, facilitating air extraction through the connecting pipe and the air hole of the column side wall, and being used for cooling the inside of the box body in the initial stage. During the air extraction process, the initial hot air in the box body is discharged, so that the inside of the box body is rapidly cooled. The connecting pipe 31 is provided with a fan 32, and the connecting pipe 31 is provided with a sealing cover 33 corresponding to the outer pipe opening side of the box cover 3.

[0031] The box cover 3 is provided with an annular groove 34 corresponding to the edge position, and the annular groove 34 is provided with a sealing ring 6. The sealing ring 6 corresponds to the upper opening side of the interlayer 11, and is used for sealing the upper side of the interlayer 11. It is used for improving the sealing property between the box cover and the box body.

[0032] As shown in the figure: Figure 5 The bottom of the box body 1 is provided with a base 7, and the inside of the base 7 is a water storage tank. The bottom of the box body 1 is provided with a water permeable hole 12 corresponding to the bottom of the interlayer 11. The water storage tank is used for receiving the water after the ice block is melted, so as to prevent the water from accumulating in the box body.

[0033] The embodiments are selected and described in the specification, in order to better explain the principles and practical application of the utility model, so that the persons skilled in the art can well understand and utilize the utility model.

Claims

1. A liquid RNA sample transport incubator, characterized in that: It comprises a cylindrical box body (1) with an open top, and a support frame (2) inside for supporting the liquid RNA sample reagent bottle; The support frame (2) can be detachably installed and is divided into multiple layers of support structure from bottom to top. Each layer of support structure comprises a support plate (21) which is circular and fixed to the same column (4) in the center. The upper side of the support plate (21) has a placing groove (22) for accommodating the reagent bottle. The upper support surface of the support plate (21) is divided into multiple fan-shaped support surfaces by a partition (23); The side wall of the box body (1) has a sandwich layer (11) which is grid-shaped corresponding to the inner side wall of the box body (1); It also comprises a box cover (3) for closing the open top of the box body (1).

2. The transport case for raw RNA samples according to claim 1, characterized in that: A tray (5) is installed at the bottom of the inner cavity of the box body (1). The tray (5) is rotatably arranged at the bottom of the box body (1) and is used for placing the support frame (2). The tray (5) has a positioning column (51) in the center. The support frame (2) has a positioning hole (24) corresponding to the lowermost support plate (21).

3. The transport case for shipping raw RNA samples of claim 1, wherein: The column (4) is a tubular structure with an inner cavity and a gas hole (41) in the side wall. The box cover (3) has a communication tube (31) installed corresponding to the upper end opening side of the column (4). The communication tube (31) and the upper end opening side of the column (4) are mutually sleeved.

4. The transport case for shipping raw RNA samples of claim 3, wherein: A fan (32) is installed in the communication tube (31). The communication tube (31) has a sealing cover (33) corresponding to the outer port side of the box cover (3) for sealing.

5. The transport case for shipping raw RNA samples of claim 1, wherein: The box cover (3) has a ring groove (34) corresponding to the edge position. A sealing ring (6) is installed in the ring groove (34) corresponding to the upper opening side of the sandwich layer (11) for sealing the upper side of the sandwich layer (11).

6. The transport case for shipping raw RNA samples of claim 1, wherein: The box body (1) has a base (7) installed at the bottom. The inner part of the base (7) is a water storage tank. The box body (1) has a water permeation hole (12) corresponding to the bottom of the sandwich layer (11).

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