Component for reaction tube body and reaction tube

Abstract

The utility model relates to a component for a reaction tube body. The second aspect of the utility model relates to a reaction tube, in particular to a reaction tube suitable for two-step reaction, such as one-tube two-step method CRISPR (clustered regularly interspaced short palindromic repeats) nucleic acid detection. The component for the reaction tube body comprises a body, the body is preferably in a hollow cone shape and comprises an upper end opening and a lower end opening hole, the size of the body is suitable for being clamped in a reaction tube, and the hole diameter of the lower end opening hole is 1-3 mm. The reaction tube comprises a tube body and a tube cover, and further comprises a part for the tube body of the reaction tube, which can be arranged in the tube body. The utility model solves the technical problem that the cover needs to be opened in two-step reaction, for example, the technical problem that the cover needs to be opened in a CRISPR detection two-step method.

Description

TECHNICAL FIELD

[0001] The first aspect of the utility model relates to a kind of components for reaction tube body;The second aspect of the utility model relates to a kind of reaction tube, especially a kind of reaction tube suitable for two-step reaction such as one pipe two-step method CRISPR nucleic acid detection. BACKGROUND

[0002] Reaction tube is the most widely used consumable in molecular diagnostic technology application, generally 0.2mL or 0.5mL with cap conical tube, made of polypropylene or similar material, usually referred to as PCR tube.PCR tube is the container for nucleic acid amplification and detection reaction such as qPCR, LAMP, RPA, CRISPR etc..During the experiment, pipette is generally used to accurately add various reaction materials, including buffer, magnesium ion, primer, probe, nucleotide, enzyme, DNA or RNA template extracted in sample etc., then cover and place in nucleic acid amplification detector of matching tube type to carry out temperature control reaction, while detecting such as fluorescence signal for judging whether the nucleic acid to be detected in sample exists.Currently this kind of reaction tube exists in the form of single tube or eight-tube product, and is mostly used in scientific research laboratory and professional clinical laboratory.

[0003] CRISPR detection, including CRISPR nucleic acid detection and CRISPR non-nucleic acid detection.CRISPR nucleic acid detection is the abbreviation of CRISPR / Cas system nucleic acid detection.CRISPR nucleic acid detection is a nucleic acid detection based on the system developed by CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and Cas protein (abbreviation of CRISPR associated protein).CRISPR nucleic acid detection includes nucleic acid detection using the cis cleavage activity of Cas protein and nucleic acid detection using the trans cleavage activity of Cas protein.CRISPR nucleic acid detection using the trans cleavage activity of Cas protein requires components including Cas protein, guide RNA and single-stranded nucleic acid and / or nucleic acid analogue reporter molecule (also referred to as nucleic acid probe).The core of this nucleic acid detection is still base pairing principle, i.e.the guide sequence of guide RNA is paired with target nucleic acid, and Cas protein acts to bind with the direct repeat sequence (DR) of guide RNA, and after the guide sequence of guide RNA is paired with target nucleic acid, it can be activated to cut activity.Cleavage activity is used by people to report signal to obtain qualitative or quantitative information of target nucleic acid detection.Therefore, Cas protein can also be regarded as part of signal reporting.

[0004] The "one-step" refers to that the nucleic acid amplification and the CRISPR nucleic acid detection are implemented in one step. The concept similar to the "one-step" is "one-pot" (also referred to as "one-pot method"). The "one-pot" refers to that the nucleic acid amplification and the CRISPR nucleic acid detection are implemented in one container, which can be a two-step method or a one-step method.

[0005] Currently, when performing the two-step reaction such as the two-step CRISPR nucleic acid detection, the reagent required for the second step reaction needs to be added after the first step reaction is completed. Practical new type content

[0006] The first aspect of the practical new type aims to provide a component for a reaction tube body, so as to solve the technical problem that the two-step experiment (such as the two-step CRISPR nucleic acid detection) needs to add the reagent required for the second step reaction after the first step reaction is completed.

[0007] The practical new type solves the above technical problem and achieves the technical effect of the practical new type by the following technical scheme.

[0008] A component for a reaction tube body, the component for the reaction tube body comprises a body, the body comprises an upper end opening and a lower end opening, the size of the body is suitable for being clamped in the body of a reaction tube, and the aperture of the lower end opening is 1-3 mm.

[0009] Preferably, the body is in a hollow conical shape.

[0010] Preferably, on the basis of any of the above technical schemes, the outer diameter of the upper end of the body is 4-6 mm, and the outer diameter of the upper end of the body matches the inner diameter of the upper part of the body of the reaction tube.

[0011] Preferably, on the basis of any of the above technical schemes, the capacity of the component for the reaction tube body is 0.5-20 μL.

[0012] Preferably, on the basis of any of the above technical schemes, the component for the reaction tube body is a reaction tube body component made of polypropylene.

[0013] Preferably, on the basis of any of the above technical schemes, the component for the reaction tube body is a white or transparent reaction tube body component.

[0014] Further to any of the above technical schemes, the component for the reaction tube body further comprises a through-hole straight pipe, the upper end of the through-hole straight pipe is in an integral structure with the lower end of the body, and the through hole of the through-hole straight pipe is in communication with the lower end opening of the body. Preferably, the length of the through-hole straight pipe is 1-5 mm, and the inner diameter of the through-hole straight pipe is the same as the aperture of the lower end opening of the body.

[0015] Further based on any of the above technical solutions, the body is provided with a through hole or is hollowed out except the bottom.

[0016] The second aspect of the utility model is to provide a reaction tube to solve the technical problem that two-step experiments (such as two-step CRISPR nucleic acid detection) need to be opened to add reagents required for the second step after completing the first step.

[0017] The utility model solves the above technical problem by the following technical scheme, and achieves the technical effect of the utility model.

[0018] A reaction tube comprises a tube body and a cover body, the cover body and the tube body are matched, and the reaction tube further comprises a reaction tube tube body component which can be arranged in the tube body, and the reaction tube tube body component is as described in any of the technical solutions of the first aspect.

[0019] The reaction tube tube body component and the reaction tube of the utility model are especially suitable for use in one-tube two-step method, such as use in one-tube two-step CRISPR nucleic acid detection, and also such as use in Argonaute protein (Ago enzyme) detection and nested PCR. The beneficial effects of the utility model are explained by taking one-tube two-step CRISPR nucleic acid detection as an example as follows: when performing the first step nucleic acid amplification reaction, the sample to be detected and the nucleic acid amplification reagent (a nucleic acid release reagent can also be added; the reagents can also be added in the form of a freeze-dried body or preloaded, and a reconstitution agent is added when used) are added in the tube body of the reaction tube, the CRISPR nucleic acid detection reagent (the CRISPR nucleic acid detection reagent can also be added in the form of a freeze-dried body or preloaded, and a reconstitution agent is added when used) is added in the reaction tube tube body component, and the cover body is covered, after the first step nucleic acid amplification reaction is completed, without opening the cover again, the CRISPR detection reagent in the reaction tube tube body component enters the bottom of the tube body of the reaction tube and mixes with the product of the nucleic acid amplification reaction through centrifugation and / or hand shaking, and the second step CRISPR nucleic acid detection can be continued, thereby preventing pollution (such as aerosol pollution) caused by opening the cover again and problems such as false positive caused thereby. BRIEF DESCRIPTION OF DRAWINGS

[0020] Figure 1 is a front view structural schematic diagram of the reaction tube tube body component of the embodiment of the utility model.

[0021] Figure 2 is a top view structural schematic diagram of the reaction tube tube body component of the embodiment of the utility model.

[0022] Figure 3 is a structural schematic diagram of the reaction tube of the embodiment of the utility model.

[0023] Figure 4is a top view structural schematic diagram of another structure of the reaction tube body component of the embodiment of the present application. DETAILED DESCRIPTION

[0024] TERMS

[0025] Unless otherwise defined, technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art.

[0026] The term "CRISPR" refers to Clustered Regularly Interspaced Short Palindromic Repeats, which is from the immune system of microorganisms.

[0027] The term "CRISPR-Cas": a unique genomic element derived from bacteria and archaea, as an adaptive immune defense system to resist invading phages or foreign nucleic acids. The system is composed of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated proteins (abbreviated as Cas proteins, Cas).

[0028] The term "Cas protein" refers to CRISPR-associated protein, which is a related protein in the CRISPR system. The "Cas protein" described herein refers to CRISPR-associated protein (some literature translates it as CRISPR-Cas effector protein, CRISPR / Cas effector protein, CRISPR-Cas effector, CRISPR / Cas effector). The Cas proteins currently detected include type I Cas protein (Cas3), type II Cas protein (Cas9), type III Cas protein (Cas10), type V Cas protein (Cas12) or type VI Cas protein (Cas13). Especially, type V Cas protein (Cas12), type VI Cas protein (Cas13) and part of Cas3, Cas10 are found to have trans-cleavage activity, which can realize signal amplification for detection, so the trans-cleavage activity is often used for detection. Taking type V Cas protein as an example, once it binds to the cis-cleavage substrate under the guidance of the guide RNA to form a ternary complex of Cas protein-guide RNA-cis-cleavage substrate, it can induce its trans-cleavage activity, i.e. random cleavage of single-stranded DNA (including base-modified single-stranded DNA), and there are also reports that it will randomly cleave single-stranded nucleic acid analogs. Of course, the cis-cleavage activity of Cas protein or other properties can also be used for detection.

[0029] The Cas protein described in the present embodiment is preferably a protein having trans-cleavage activity. In particular, the Cas protein is active, especially trans-cleavage active, at a temperature higher than the temperature of the system in which the isothermal amplification reaction is performed.

[0030] The term "Cas12a" (formerly "Cpf1") is a crRNA-dependent endonuclease, which is a V-A type enzyme in the classification of CRISPR systems.

[0031] The term "Cas12b" (formerly "C2c1") is a sgRNA-dependent endonuclease, which is a V-B type enzyme in the classification of CRISPR systems.

[0032] The term "PAM" refers to a protospacer-adjacent motif, which is a short DNA sequence directly adjacent to the DNA sequence targeted by the CRISPR effector protein, and is necessary for Cas12a or Cas12b to cleave double-stranded DNA. For example, the PAM of Cas12a is TTTV, and the PAM of AacCas12b is a TTN sequence.

[0033] The term "target DNA or RNA molecule" refers to a DNA or RNA or a specific portion thereof to be tested when the nucleic acid molecule to be tested is a nucleic acid molecule; when the non-nucleic acid molecule to be tested is a nucleic acid sequence designed in advance.

[0034] The term "CRISPR nucleic acid detection method" refers to a nucleic acid detection method using a Cas protein, including a nucleic acid detection method using the Cas protein for cis-cleavage activity, trans-cleavage activity, or other functions.

[0035] The term "one-step CRISPR nucleic acid detection method (using Cas protein transcleavage activity)" (or simply CRISPR one-step nucleic acid detection, CRISPR one-step, one-step detection, one-step) is a rapid and convenient detection technology developed on the basis of the CRISPR nucleic acid detection system, which can simultaneously realize the amplification and detection of target nucleic acid in one reaction tube. This technology combines CRISPR-Cas system and isothermal amplification (or constant temperature amplification) technology, and does not need to open the cover operation after amplification of nucleic acid product, which can specifically detect the target nucleic acid in a short time. The CRISPR one-step detection technology is a rapid, accurate, high sensitivity and high specificity detection technology, which is not only simple to operate, but also can improve the detection specificity of the current isothermal amplification technology. Compared with the traditional PCR technology, the CRISPR one-step detection does not need complex temperature control and multi-step operation, and has higher real-time and portability. The Chinese invention patent with application publication number CN 110551800 A and application publication date 2019.12.10 first discloses one-step method (see

[0238] ,

[0239] and other paragraphs of the patent application).

[0036] The term "system" should be understood broadly, which can be a composition, a product combination, a reagent, a kit, an instrument containing the aforementioned composition, product combination, reagent, kit, a mixture (system) formed when the composition, product combination, reagent, kit is used for detection, and an instrument containing the aforementioned mixture, etc.

[0037] The term "temperature" refers to the temperature of the system (mixture formed when used for detection).

[0038] The term "guide RNA" is a mature crRNA fused (or not fused) with tracrRNA as a guide RNA, or a mature crRNA fused (or not fused) with scoutRNA as a guide RNA, or a crRNA alone as a guide RNA.

[0039] Generally, a guide RNA (gRNA) can comprise, consist essentially of, or consist of direct repeat sequences (also referred to as DR sequences) and a guide sequence. The gRNA can include crRNA and tracrRNA, crRNA and scoutRNA, or only crRNA in different Type V CRISPR systems depending on the Cas protein it relies on. The crRNA and tracrRNA can be artificially engineered to form a single guide RNA (sgRNA). In some cases, the guide sequence is a polynucleotide sequence that is of sufficient complementarity to hybridize to and direct specific binding of a CRISPR / Cas protein-guide RNA complex to a cis-cleavage substrate nucleic acid, typically having a sequence length of 15-28 nt in Type V CRISPR systems. The direct repeat sequences can fold to form a specific structure (e.g., stem-loop structure) for recognition by the Cas protein to form a complex. The guide sequence does not need to be 100% complementary to the cis-cleavage substrate nucleic acid. The guide sequence is not complementary to the nucleic acid in the trans-cleavage reporter molecule.

[0040] In some embodiments, the degree of complementarity (match) between the guide sequence and its corresponding cis-cleavage substrate nucleic acid, when optimally aligned, is at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, or at least 99%. Determining optimal alignment is within the capabilities of a person of ordinary skill in the art. For example, there are publicly available and commercially available alignment algorithms and programs, such as, but not limited to, ClustalW, Smith-Waterman in matlab, Bowtie, Geneious, Biopython, and SeqMan.

[0041] The terms “polynucleotide,” “nucleotide sequence,” “nucleic acid sequence,” “nucleic acid molecule,” and “nucleic acid” can be used interchangeably and encompass DNA, RNA, or hybrids thereof, which can be double-stranded or single-stranded, unless indicated otherwise.

[0042] The terms "homology" or "identity" are used in reference to the matching of sequences between two polypeptides or between two nucleic acids. When a position in each of two sequences being compared is occupied by the same base or amino acid monomer subunit (e.g., a position in each of two DNA molecules occupied by adenine, or a position in each of two polypeptides occupied by lysine), then the molecules are identical at that position. Generally, comparisons are made using complete sequences aligned for maximum identity. Such alignments can be determined by computerized running of algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group) using, for example, the default parameters of the programs. BLAST algorithms available from the National Center for Biotechnology Information (NCBI www.ncbi.nlm.nih.gov / ) can also be used, using the default parameters.

[0043] The term "nucleic acid analogues" is a class of derivatives of RNA and DNA, which is mainly composed of phosphate, pentose and base, while the nucleic acid analogue replaces at least one of them with some other substance. The main nucleic acid analogues are peptide nucleic acid (PNA), morpholino (MNA), bridged nucleic acid (BNA), locked nucleic acid (LNA), glycol nucleic acid (GNA) and threose nucleic acid (TNA). Some of these nucleic acid analogues can even carry out biological processes such as replication, translation, etc. in vitro (Brudno, Yevgeny; Birnbaum, Michael E; Kleiner, Ralph E; Liu, David R. "An in vitro translation, selection and amplification system for peptide nucleic acids". Nature Chemical Biology. 6 (2): 148-155. doi:10.1038 / nchembio.280. PMC 2808706. PMID 20081830).

[0044] The term "test sample" refers to a sample obtained from a biological sample that has been subjected to extraction of nucleic acids, which sample can also have been subjected to amplification, transcription, reverse transcription of nucleic acids. The biological sample is any solid or fluid sample obtained, excreted or secreted from any organism, including but not limited to unicellular organisms, such as bacteria, yeast, protozoa, and amoebae, and multicellular organisms (e.g., plants or animals, including samples from a healthy or apparently healthy human subject or a human patient affected by a condition or disease to be diagnosed or investigated, e.g., an infection by a pathogenic microorganism, such as a pathogenic bacterium or virus). For example, the biological sample can be a biological fluid obtained from, e.g., blood, plasma, serum, urine, feces, sputum, mucus, lymph, synovial fluid, bile, ascites, pleural effusion, seroma, saliva, cerebrospinal fluid, aqueous or vitreous fluid, or any bodily secretion, exudate, exudate (e.g., fluid obtained from an abscess or any other site of infection or inflammation), or fluid obtained from a joint (e.g., a normal joint or a joint affected by a disease, such as rheumatoid arthritis, osteoarthritis, gout, or septic arthritis), or a swab of a skin or mucosal surface. The sample can also be a sample obtained from any organ or tissue (including a biopsy or autopsy specimen, such as a tumor biopsy) or can comprise cells (primary cells or cultured cells) or a medium conditioned by any cell, tissue, or organ. Exemplary samples include, but are not limited to, cells, cell lysates, blood smears, cell centrifugation preparations, cytological smears, body fluids (e.g., blood, plasma, serum, saliva, sputum, urine, bronchoalveolar lavage, semen, etc.), tissue biopsies (e.g., tumor biopsies), fine needle aspirates, and / or tissue sections (e.g., cryostat tissue sections and / or paraffin-embedded tissue sections).

[0045] In other embodiments, the biological sample can be a plant cell, callus, tissue, or organ (e.g., roots, stems, leaves, flowers, seeds, fruits), etc.

[0046] The "sample to be tested" can contain a nucleic acid molecule to be tested. In the present application, the nucleic acid molecule to be tested includes a DNA molecule, and also includes an RNA molecule or a DNA molecule formed by reverse transcription of an RNA molecule, or further, the nucleic acid molecule to be tested can be amplified by a technique known in the art, and the amplification technique is an isothermal amplification technique, and the isothermal amplification can be LAMP (loop-mediated isothermal amplification), RPA (recombinase polymerase amplification), RAA (recombinase-mediated amplification), ERA (enzyme recombination isothermal amplification technique), MIRA (multi-enzyme isothermal rapid amplification technique), bDNA (branch DNA amplification), NASBA (nucleic acid sequence-based amplification), SDA (strand displacement amplification), TMA (transcription-mediated amplification), RCA (rolling circle amplification), HDA (helicase-dependent amplification), SPIA (single primer isothermal amplification), NEAR (nicking enzyme amplification reaction), SMAP (smart amplification process), SMAP2 (second edition of smart amplification process), CPA (crossover primer amplification), MDA (multiple displacement amplification), RAM (Ramification), cHDA (helicase-dependent circular amplification), SMART (signal-mediated amplification of RNA technique), 3SR (self-sustained sequence replication system), GEAR (genomic exponential amplification reaction), IMDA (isothermal multiple displacement amplification), ERA (enzyme recombination isothermal amplification), TAS (transcription-dependent amplification system), RIDA (rapid isothermal detection amplification technique), NEMA (nicking endonuclease isothermal amplification of nucleic acids), EXPAR (exponential isothermal amplification), ICAN (isothermal chimeric primer-initiated amplification of nucleic acids), SEA (strand exchange amplification), SHARP (SSB-helicase-mediated rapid PCR), IMSA (isothermal multiple self-priming amplification), WGA (whole genome amplification), PSR (polymerase spiral reaction), or a combination thereof.

[0047] Further, the detection method of the present application further comprises a step of amplifying the nucleic acid molecule to be tested; and the detection system further comprises components for amplifying the nucleic acid molecule to be tested. The components for amplification include one or more of the following: a DNA polymerase, a reverse transcriptase, a strand displacement enzyme, a nicking endonuclease, a helicase, a recombinase, a single-strand binding protein, a recombination modulating protein, a T7 RNA polymerase, an RNase H, dNTPs for amplification reaction and / or reverse transcription reaction, NTPs for transcription reaction, a buffer, and the like.

[0048] The term "Ago protein" is an Argonaute protein.

[0049] The term "Ago protein nucleic acid detection" refers to nucleic acid detection using Argonaute protein, such as "Nucleic acid detection method based on prokaryotic Argonaute protein and its application" disclosed in Chinese invention patent CN108796036A, "Nucleic acid detection method based on normal temperature prokaryotic Argonaute protein and its application" disclosed in CN114277109A, "Visual detection system, reagent or kit for detecting target nucleic acid molecules and detection method" disclosed in CN114085892A, and "Nucleic acid detection method based on medium temperature Argonaute protein and isothermal amplification" disclosed in CN116064736A. In addition, short pAgo and its related nuclease effect protein can form a heterodimer complex (TmuRE-Ago complex); unlike long pAgo which specifically cleaves target DNA, this complex is activated after RNA-guided DNA target recognition, exhibiting high non-specific DNA cleavage activity (see https: / / doi.org / 10.1093 / nar / gkad1145). This non-specific DNA cleavage activity can also be used for detection.

[0050] The specific embodiment proposes a component for reaction tube body, and on this basis proposes a single tube, eight-tube array tube and other product forms of reaction tube.

[0051] As shown in Figure 1 , 2 , a component for reaction tube body 1, the component for reaction tube body 1 comprises a body 11, the body 11 is hollow conical, comprising an upper end opening 110 and a lower end opening 111, the size of the body 11 is suitable for being clamped in the tube body 2 of the reaction tube, and the aperture of the lower end opening 111 is 1-3 mm. The outer diameter of the upper end of the body 11 is 4-6 mm. The outer diameter of the upper end of the body 11 matches the inner diameter of the upper part of the tube body 2 of the reaction tube, that is, the body 11 is suitable for being clamped in the tube body 2 of the reaction tube. The capacity of the component for reaction tube body 1 is 0.5-20 μL. The component for reaction tube body 1 further comprises a through-hole straight tube 12, the upper end of the through-hole straight tube 12 is in an integral structure with the lower end of the body 11, and the through-hole of the through-hole straight tube 12 communicates with the lower end opening of the body 11. Preferably, the length of the through-hole straight tube 12 is 1-5 mm, and the inner diameter of the through-hole straight tube 12 is the same as the aperture of the lower end opening 111 of the body 11. As shown in Figure 2 , the body 11 is provided with a through-hole 112. As shown in Figure 4 , in another embodiment, the body 11 is hollow except the bottom and comprises four hollow holes 113, of course, it can also be two, three, five, etc. Figure 4 The middle of the body 11 is the lower end opening 111. The through-hole 112 or the hollow hole 113 facilitates adding reagents through the body 11 into the tube body 2 of the reaction tube in use.

[0052] The reaction tube body component 1 is a reaction tube body component made of polypropylene.

[0053] A reaction tube has the following preferred structure as a single tube. Figure 3 As shown in the figure, a reaction tube includes a reaction tube body component 1, a tube body 2, a cover body 3, and a connecting portion 4, the tube body 2 includes a tube body opening, the cover body 3 can cover the tube body 2 opening, and the reaction tube body component 1 can be clamped in the tube body 2 of the reaction tube. The reaction tube can also include a nucleic acid amplification reaction reagent freeze-dried body arranged in the tube body 2 (not shown in the figure), and the reaction tube also includes a sealing film (not shown in the figure) in this technical solution. The reaction tube can also include a connecting portion 4, and the cover body 3 is connected to the tube body 2 through the connecting portion 4.

[0054] The reaction tube of the utility model can also be made into an eight-row reaction tube. An eight-row reaction tube includes eight plastic transparent reaction tubes in a row, and the plastic transparent reaction tube is the reaction tube of any of the above technical solutions.

[0055] The reaction tube body component and the reaction tube of the utility model are especially suitable for use in a one-tube two-step method, such as a one-tube two-step method for CRISPR nucleic acid detection, and also such as Argonaute protein (Ago enzyme) detection and nested PCR. The beneficial effects of the utility model are described below by taking the one-tube two-step method for CRISPR nucleic acid detection as an example: when performing the first step of nucleic acid amplification reaction, the sample to be detected and the nucleic acid amplification reaction reagent (a nucleic acid release reagent can also be added; the reagents can also be added in the form of a freeze-dried body or preloaded, and a reconstitution agent is added when used) are added to the tube body 2 of the reaction tube, the CRISPR nucleic acid detection reagent (the CRISPR nucleic acid detection reagent can also be added in the form of a freeze-dried body or preloaded, and a reconstitution agent is added when used) is added to the reaction tube body component 1, and the cover body 3 is covered. After the first step of nucleic acid amplification reaction is completed, without opening the cover body 2 again, the CRISPR detection reagent in the reaction tube body component 1 is mixed with the product of the nucleic acid amplification reaction at the bottom of the tube body 2 of the reaction tube by centrifugation and / or hand shaking, and the second step of CRISPR nucleic acid detection can be continued, thereby preventing pollution (such as aerosol pollution) caused by opening the cover body 2 again and problems such as false positives caused by the pollution.

Claims

1. A component for a reaction tube body, characterized by, The reaction tube body part includes a body having an upper end opening and a lower end aperture, the body being sized to fit into the tube body of the reaction tube, the lower end aperture having a hole diameter of 1-3 mm.

2. The component for a reaction tube body according to claim 1, characterized by The body is hollow conical.

3. The component for a reaction tube body according to claim 1 or 2, characterized by The upper end outer diameter of the body is 4-6 mm, and the upper end outer diameter of the body matches the upper inner diameter of the tube body of the reaction tube.

4. The component for a reaction tube body according to claim 1 or 2, characterized by The capacity of the reaction tube body part is 0.5-20 μL.

5. The component for a reaction tube body according to claim 1 or 2, characterized by The reaction tube body part is made of polypropylene.

6. The component for a reaction tube body according to claim 1 or 2, characterized by The reaction tube body part is white or transparent.

7. The component for a reaction tube body according to claim 1 or 2, characterized by The reaction tube body part further includes a through-hole straight pipe, the upper end of the through-hole straight pipe being in one-piece structure with the lower end of the body, and the through-hole of the through-hole straight pipe being in communication with the lower end aperture of the body.

8. The reaction tube member according to claim 7, wherein The through-hole straight pipe is 1-5 mm long, and the inner diameter of the through-hole straight pipe is the same as the hole diameter of the lower end aperture of the body.

9. The component for a reaction tube body according to claim 1 or 2, characterized by The body is provided with a through-hole or is hollow except the bottom.

10. A reaction tube comprising a tube body and a cap body, the cap body and the tube body being adapted to fit together, characterised in that, The reaction tube further includes a reaction tube body part that can be arranged in the tube body, the reaction tube body part being as claimed in any one of claims 1-9.

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