One-step microdroplet manufacturing embryo culture dish

Abstract

The utility model discloses a one-step microdroplet manufacturing embryo culture dish. The one-step microdroplet manufacturing embryo culture dish comprises a dish body and a plurality of microdroplet preparation grooves formed in the dish body, the micro-droplet preparation tank comprises a central liquid collecting tank and a plurality of micro-pore tanks which are arranged on the peripheral side of the central liquid collecting tank in a surrounding manner; wherein the central liquid collecting tank is higher than the micro-pore tank, and a liquid separation flow channel for overflowing is arranged between the central liquid collecting tank and the micro-pore tank. According to the utility model, liquid only needs to be filled into the liquid collecting groove in the center of the dish body, and the liquid can flow into the micro-pore grooves in the periphery through the liquid separating flow channel; liquid with the same volume is added into the multiple micropores in one step, one-step microdroplet manufacturing is achieved by means of the height difference of the micropores, and multiple microdroplets can be manufactured by only adding the liquid once; the operation time is obviously shortened, the operation efficiency is improved, and the influence of liquid drop volume volatilization on embryo culture is reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to embryo culture dish technical field especially, relate to a one-step microdrop preparation embryo culture dish. BACKGROUND

[0002] The conventional method of human embryo culture is microdrop method, that is, several drops of culture solution (volume about 20-50 microliters) are prepared in a culture dish, and a single or several embryos are cultured in each drop. The operator needs to prepare dozens to hundreds of microdrop culture dishes every day. The operator usually uses a glass Pasteur pipette without scale mark to prepare the drops. It is difficult to ensure the consistency of drop volume, and the preparation process is slow. The small drop volume is easy to evaporate, which will change the osmotic pressure and affect the embryo culture effect.

[0003] In the prior art, patent application No. 202122490052.X provides a culture dish for assisted reproduction, which comprises a culture dish body and a dish cover arranged on the culture dish body. The culture dish body is provided with a cylindrical accommodating cavity. A circular ovum accommodating groove is arranged in the middle of the accommodating cavity. A plurality of circular sperm accommodating grooves are arranged on the inner side of the periphery of the accommodating cavity. A communication passage is arranged between the sperm accommodating grooves and the ovum accommodating groove. The bottom of the ovum accommodating groove is arranged to be higher than the sperm accommodating grooves in the height direction, so that the passage is formed in an inclined manner. The passage is provided with a first blocking slope at the outlet of the sperm accommodating groove, and a second blocking slope is arranged at the inlet of the ovum accommodating groove. The utility model can realize the screening of high-quality sperm, so as to ensure that high-quality fertilized eggs are obtained.

[0004] In the prior art, the existing embryo culture dish needs to be prepared drop by drop, and the process is slow. Taking a commonly used culture dish (diameter about 3.5 cm) as an example, 16 drops (4 rows and 4 columns) are prepared, which takes about 16 seconds. In addition, it is difficult to control the volume of the drops prepared by manual use of the Pasteur pipette, and it is difficult to ensure that all the drops have the same volume. Moreover, the existing microporous culture dish has no liquid scale mark, and the existing embryo culture dish is tightly combined with the dish cover, which is not conducive to gas exchange. UTILITARY MODEL

[0005] The utility model aims at providing a one-step microdrop preparation embryo culture dish to solve the above technical problems in the use of conventional microdrop preparation embryo culture dishes.

[0006] To solve the above technical problems, the utility model adopts the following technical scheme:

[0007] The utility model provides a one-step microdrop preparation embryo culture dish, which comprises a dish body and a plurality of microdrop preparation grooves arranged on the dish body.

[0008] The micro-droplet preparation tank comprises a center collecting tank and a plurality of micro-hole tanks arranged around the periphery of the center collecting tank; wherein the center collecting tank is higher than the micro-hole tanks, and a liquid distribution flow channel for overflow is arranged between the center collecting tank and the micro-hole tanks.

[0009] Further, the liquid distribution flow channel is inclined and arranged between the center collecting tank and the micro-hole tanks.

[0010] Still further, the micro-hole tank comprises a cylindrical tank connected with the liquid distribution flow channel and a circular truncated cone tank coaxially communicated with the cylindrical tank.

[0011] Still further, an annular scale line is arranged in the micro-hole tank.

[0012] Still further, a partition plate is arranged between adjacent micro-hole tanks; the partition plate is an annular structure.

[0013] Still further, a dish cover is arranged on the dish body, and an abrasive surface area is arranged on the top surface of the dish cover.

[0014] A plurality of limiting columns are symmetrically arranged on the inner wall of the dish cover.

[0015] Compared with the prior art, the beneficial technical effects of the present application are:

[0016] 1. In the present application, only liquid needs to be added in the center collecting tank of the dish body, and the liquid will flow to the surrounding micro-hole tanks through the liquid distribution flow channel; one-step addition of the same volume of liquid into multiple micro-holes is realized, one-step micro-droplet preparation is realized by utilizing the height difference of the micro-holes, and multiple micro-droplets can be prepared only by adding liquid once; the operation time is obviously shortened, the operation efficiency is improved, and the influence of droplet volume evaporation on embryo culture is reduced.

[0017] 2. Compared with the conventional manual preparation of micro-droplets one by one, the size of the droplets is difficult to be uniform, and this structure improves the consistency of the droplet volume.

[0018] 3. The lateral cross section of the culture dish is used for pasting a chip for identifying the identity of a patient, so that the chip is pasted on the dish body rather than the dish cover.

[0019] 4. The frosted design of the dish cover can facilitate the identification of the patient's name in the incubator in a dim environment. The frosted part does not block the embryo, and the embryo can be observed under the microscope with the cover. BRIEF DESCRIPTION OF DRAWINGS

[0020] The present application will be further described below in conjunction with the drawings.

[0021] Figure 1 It is a top view schematic diagram of the one-step micro-droplet preparation embryo culture dish of the present application.

[0022] Figure 2 It is a partial top view schematic diagram of the one-step micro-droplet preparation embryo culture dish of the utility model;

[0023] Figure 3 It is Figure 2 The structure section schematic diagram;

[0024] Figure 4 It is the installation position schematic diagram of the center liquid collecting groove and the micro-hole groove on the one-step micro-droplet preparation embryo culture dish of the utility model;

[0025] Figure 5 It is the top surface structure schematic diagram of the dish cover on the one-step micro-droplet preparation embryo culture dish of the utility model;

[0026] Figure 6 It is the internal structure schematic diagram of the dish cover on the one-step micro-droplet preparation embryo culture dish of the utility model.

[0027] The figure mark explanation: 100, dish body; 200, micro-droplet preparation groove; 201, center liquid collecting groove; 202, liquid distribution flow channel; 203, micro-hole groove; 204, partition plate; 2031, cylindrical groove; 2032, circular truncated cone groove; 2033, scale line; 300, dish cover; 301, frosted surface area; 302, limiting column. Specific implementation

[0028] Disclosed in the embodiment is a one-step micro-droplet preparation embryo culture dish, which comprises a dish body 100 and a plurality of micro-droplet preparation grooves 200 formed on the dish body 100.

[0029] Specifically, the micro-droplet preparation groove 200 comprises a center liquid collecting groove 201 and a plurality of micro-hole grooves 203 surrounding the outer peripheral side of the center liquid collecting groove 201; wherein the center liquid collecting groove 201 is higher than the micro-hole grooves 203, and a liquid distribution flow channel 202 for overflow is arranged between the center liquid collecting groove 201 and the micro-hole grooves 203.

[0030] As shown in the drawing, Figure 3 The liquid distribution flow channel 202 is inclinedly arranged between the center liquid collecting groove 201 and the micro-hole grooves 203. The micro-hole groove 203 comprises a cylindrical groove 2031 connected with the liquid distribution flow channel 202 and a circular truncated cone groove 2032 coaxially communicated with the cylindrical groove 2031; wherein the top inner diameter of the circular truncated cone groove 2032 is greater than the bottom inner diameter thereof, and the top of the circular truncated cone groove 2032 is communicated with the bottom of the cylindrical groove 2031.

[0031] In use, only liquid needs to be added in the center liquid collecting groove 201 on the dish body 100, and the liquid will flow to the surrounding micro-hole grooves 203 through the liquid distribution flow channel 202; wherein 16 micro-droplets are prepared for about 2s. Figure 1 And Figure 2As shown, a microporous structure is used to add the same volume of liquid into eight micropores in one step.

[0032] In this embodiment, an annular scale line 2033 is pre-formed within the microporous groove 203. Specifically, a first scale line and a second scale line (in a specific implementation, the scale lines can represent 20 μL and 30 μL respectively) are pre-formed within the microporous groove 203, wherein the first scale line is higher than the second scale line. The annular scale line pre-formed within the microporous groove 203 facilitates observation during operation.

[0033] In this embodiment, a partition 204 is installed between adjacent microporous grooves 203; the partition 204 is an annular structure; it is used to prevent the liquid from communicating with each other due to surface tension when the liquid level in adjacent microporous grooves 203 is too high.

[0034] like Figure 5 As shown, in this embodiment, a lid 300 is disposed on the dish body 100, and the top surface of the lid 300 is pre-fabricated with a frosted surface area 301, in which a mark or name is written using the friction surface.

[0035] like Figure 6 As shown, in this embodiment, four limiting posts 302 are symmetrically installed on the inner wall of the dish lid 300. When the dish lid 300 is placed on the dish body 100, there is a 1mm gap between them. The dish lid 300 is circular, while the dish body 100 is not circular (e.g., ...). Figure 1 As shown in the figure, which has a cross-section, these structures significantly increase the gas exchange area.

[0036] Usage process and working principle:

[0037] Petri dish body:

[0038] The culture dish 100 has a diameter of 35 mm and a height of 10 mm. The dish contains three sets of microdroplet preparation tanks 200, each with eight micropores 203. The number of microdroplets to be prepared (8, 16, or 24) is selected according to the required number of microdroplets.

[0039] The micropore groove 203 is used for embryo culture; the micropore groove 203 has a variable diameter structure, and includes a cylindrical groove 2031 connected to the liquid distribution channel 202 and a frustum-shaped groove 2032 coaxially connected to the cylindrical groove 2031; its bottom inner diameter is smaller than its upper inner diameter, which is used to limit the position of the embryo for easy location.

[0040] The cylindrical groove 2031 is an approximately elliptical hollow cylinder. The horizontal cross-section of the cylindrical groove 2031 is approximately elliptical, with a length of 4.7 mm, a width of 3.6 mm, and a height of 2.7 mm. The upper volume of the microporous groove 203 is approximately 34 μL.

[0041] The upper opening of the circular-truncated-cone-shaped groove 2032 is circular with a diameter of 2 mm, and the bottom is circular with a diameter of 1.5 mm and a depth of 1 mm, and the volume of the lower part of the micro-hole groove 203 is about 1.8 ul.

[0042] The central collection groove 201 is a central pool, a cylinder with a diameter of 6 mm and a depth of 3 mm, and the volume is about 85 ul. Eight identical cylindrical grooves 2031 are distributed around the central collection groove 201. After adding about 325 ul of liquid to the central collection groove 201, the central collection groove 201 (about 85 ul) is filled, and the liquid will flow uniformly to the lower cylindrical groove 2031 (about 30 ul).

[0043] In this embodiment, the slope surface distribution flow channel 202 is connected between the central collection groove 201 and the cylindrical groove 2031, and the upper edge of the central collection groove 201 is 1.8 mm higher than the upper edge of the cylindrical groove 2031, and the horizontal distance is 0.8 mm.

[0044] In this embodiment, a partition is installed between adjacent micro-hole grooves 203 to prevent mixing of adjacent liquids. In this embodiment, the cross section of the dish body 100 is used to paste a chip for identifying the identity of the patient. The cross section width is 18 mm.

[0045] In this embodiment, the diameter of the dish cover 300 is 36 mm, and the height is 5 mm. The semicircular frosted area on the outer surface of the dish cover is 32 mm wide and 9.8 mm high. It is used for writing the patient's name. The frosted surface 301 helps to identify the patient's name in the incubator. In this embodiment, the frosted area of the frosted surface 301 does not block the droplets, and does not affect the observation of embryos in the droplets through the dish cover 300.

[0046] The inner surface of the dish cover 300 is provided with four protruding short columns 302 (1.5 mm long, 1 mm wide, and 1 mm high) for increasing the air permeation space between the dish cover and the dish body.

[0047] The above embodiments only describe the preferred mode of the present application, and do not limit the scope of the present application. Without departing from the design spirit of the present application, various modifications and improvements to the technical solutions of the present application made by those skilled in the art shall fall within the protection scope determined by the claims of the present application.

Claims

1. A one-step microdrop embryo culture dish, characterized by: The dish body (100) is provided with a plurality of micro-droplet preparation grooves (200). The micro-droplet preparation groove (200) comprises a central collecting groove (201) and a plurality of micropore grooves (203) arranged around the periphery of the central collecting groove (201); wherein the central collecting groove (201) is higher than the micropore grooves (203), and a distribution flow channel (202) for overflow is arranged between the central collecting groove (201) and the micropore grooves (203).

2. The one-step microdroplet fabrication embryo culture dish of claim 1, wherein: The distribution flow channel (202) is inclined and arranged between the central collecting groove (201) and the micropore grooves (203).

3. The one-step microdroplet fabrication embryo culture dish of claim 2, wherein: The micropore groove (203) comprises a cylindrical groove (2031) connected with the distribution flow channel (202) and a circular truncated cone groove (2032) coaxially communicated with the cylindrical groove (2031).

4. The one-step microdroplet fabrication embryo culture dish of claim 3, wherein: An annular scale line (2033) is arranged in the micropore groove (203).

5. The one-step microdroplet fabrication embryo culture dish of claim 4, wherein: A partition plate (204) is arranged between adjacent micropore grooves (203); the partition plate (204) is an annular structure.

6. The one-step microdroplet fabrication embryo culture dish of claim 5, wherein: A dish cover (300) is arranged on the dish body (100), and a frosted surface area (301) is arranged on the top surface of the dish cover (300).

7. The one-step microdroplet fabrication embryo culture dish of claim 6, wherein: A plurality of limiting columns (302) are symmetrically arranged on the inner wall of the dish cover (300).

Images