Western blot membrane washing box capable of being disassembled and assembled

Abstract

The utility model provides a detachably assembled western blot washing box which comprises an upper box body assembly and a lower box body assembly, the upper box body assembly comprises an upper box body or a plurality of upper box bodies which are detachably arranged in parallel, and the lower box body assembly comprises a lower box body or a plurality of lower box bodies which are detachably arranged in parallel; the lower box body corresponds to the upper box body and is detachably installed at the bottom of the upper box body, a flow channel used for being communicated with the lower box body is formed in the bottom of the upper box body, a baffle corresponding to the flow channel is arranged on the top of the lower box body in a sliding mode, and the baffle is used for opening or closing the flow channel in a sliding mode. According to the utility model, the spliced membrane washing boxes are adopted, so that the defects and waste of compartments are avoided, membrane washing boxes of various specifications do not need to be purchased, waste liquid does not need to be discarded every time in a membrane washing link, the waste liquid can be temporarily stored in the lower box body, and the waste liquid is transferred after the whole membrane washing is finished, so that an experimenter and an operation environment can be protected, and the experiment efficiency is improved. And the film washing time can be shortened.

Description

TECHNICAL FIELD

[0001] The utility model relates to a washing film box technical field, specifically, relates to a detachable assembly's protein imprinting washing film box. BACKGROUND

[0002] Protein immunoblotting is a kind of immunological detection technology, the method is transferred to solid phase membrane from protein gel, and the purpose protein is detected by antibody antigen combination. The important steps of the experiment, such as solid phase membrane cleaning, antibody incubation combination, antibody recovery, etc. Involved the transfer of solution, therefore need to have suitable container to provide the place of these steps.

[0003] At present, ordinary circular dish or different specifications washing film box of different sizes are used in the steps of washing film, antibody antigen combination. Different specifications washing film box, the main body structure is multiple cuboid compartments, each compartment can carry a piece of strip-shaped solid phase membrane to carry out independent operation in the experimental process, and one washing film box is generally composed of 4 or 5 compartments. The number of solid phase membranes incubated by protein imprinting is determined based on the number of protein markers detected each time, and the fixed specification washing film box will often appear the embarrassing situation of not enough for one washing film box and too much for two washing film boxes. At the same time, in each liquid transfer step, the traditional washing film box generally adopts the way of pouring liquid or using a suction head to move liquid, but the former is easy to spill on the operation table and the experimental operator, and the latter will cause the increase of the number of suction heads (generally 5ml specification suction head) and the increase of operation time, which is easy to cause the solid phase membrane to dry and affect the experimental results. UTILITY MODEL CONTENTS

[0004] In view of the defects in the prior art, the utility model aims at providing a detachable assembly's protein imprinting washing film box.

[0005] According to the detachable assembly's protein imprinting washing film box provided by the utility model, including upper box body assembly and lower box body assembly, the upper box body assembly includes one upper box body or a plurality of parallel detachable upper box bodies, the lower box body assembly includes one lower box body or a plurality of parallel detachable lower box bodies;

[0006] The lower box body corresponds to the upper box body and is detachably installed at the bottom of the upper box body, the bottom of the upper box body is provided with a flow channel for communication with the lower box body, the top of the lower box body is slidably provided with a baffle corresponding to the flow channel, and the baffle is used to open or close the flow channel by sliding.

[0007] Preferably, the two sides of the top of the upper box body are respectively provided with a first side part clamping groove and a second side part clamping groove;

[0008] The upper box bodies are connected by the first side slot and the second side slot of the upper box body on one side, and the second side slot and the first side slot of the upper box body on the other side.

[0009] Preferably, the bottom of the upper box body is provided with a bottom slot on both sides, and the top of the lower box body is provided with a first top slot and a second top slot on both sides.

[0010] The upper box body is connected by the first top slot and the second top slot of the lower box body top on both sides through the bottom slot on both sides.

[0011] Preferably, the top of the lower box body is provided with a third side slot and a fourth side slot on both sides.

[0012] The lower box bodies are connected by the fourth side slot and the third side slot of the lower box body on one side, and the third side slot and the fourth side slot of the lower box body on the other side.

[0013] Preferably, the top of the upper box body is provided with a cover, and the rear end of the cover is rotatably connected with the rear end of the upper box body through a knob.

[0014] Preferably, the front end of the cover is provided with a cover buckle, and the front end of the upper box body is provided with a box buckle corresponding to the cover buckle.

[0015] The upper box body is connected by the cover buckle and the box buckle on the front end of the upper box body.

[0016] Preferably, the top of the baffle is provided with a push and twist, and the two sides of the baffle are slidingly arranged in the slide on both sides of the top inside the lower box body.

[0017] Preferably, the rear side of the baffle is provided with a comb structure, and the baffle and the comb structure can reciprocally slide between a first position and a second position.

[0018] In the first position, the flow channel at the bottom of the upper box body is completely coincident with the baffle in the projection of the top view, and the flow channel is closed by the baffle.

[0019] In the second position, the baffle is located on the front side of the flow channel at the bottom of the upper box body in the projection of the top view, and the baffle and the flow channel are not coincident, the flow channel is opened, and the comb structure is located directly below the flow channel.

[0020] Preferably, the top of the lower box body is provided with a vertical limiting plate, and the rear side of the baffle is provided with a limiting part for limiting the rear side of the vertical limiting plate.

[0021] When the limiting part is overlapped and limited with the vertical limiting plate, the baffle and the comb tooth structure are located at the second position.

[0022] Preferably, the top of the baffle is provided with a sealing ring circumferentially along the bottom flow channel of the upper box body, for sealing the flow channel.

[0023] Compared with the prior art, the utility model has the beneficial effects that:

[0024] The utility model discloses simple structure, convenient operation adopts the washable membrane box of splicing, does not cause the shortage of compartment and waste, also need not to buy multiple specifications washable membrane box, and in the washable membrane link, need not to discard waste liquid every time, can temporarily store waste liquid in the lower box body, after the whole washable membrane ends, then shift waste liquid, can protect the experimental personnel and operating environment, also can shorten the time of washable membrane. BRIEF DESCRIPTION OF DRAWINGS

[0025] Other features, objects and advantages of the utility model will become more apparent through reading the following detailed description of the non-restrictive embodiments with reference to the accompanying drawings:

[0026] Figure 1 It is the whole structure schematic diagram of the utility model;

[0027] Figure 2 It is the structure schematic diagram of the upper box body assembly in the utility model;

[0028] Figure 3 It is the structure schematic diagram of the upper box body in the utility model in the overhead angle;

[0029] Figure 4 It is the structure schematic diagram of the upper box body in the utility model in the main view angle;

[0030] Figure 5 It is the structure schematic diagram of the upper box body in the utility model in the left view angle;

[0031] Figure 6 It is the structure schematic diagram of the upper box body in the utility model in the right view angle;

[0032] Figure 7 It is the structure schematic diagram of the lower box body assembly in the utility model;

[0033] Figure 8 It is the structure schematic diagram of the lower box body in the utility model in the overhead angle;

[0034] Figure 9 It is the structure schematic diagram of the lower box body in the utility model in the main view angle;

[0035] Figure 10 It is the structure schematic diagram of the lower box body in the utility model in the left view angle;

[0036] Figure 11 It is the structure schematic view of the right angle of the lower box body in the utility model.

[0037] As shown in the figure:

[0038] Upper box body 1 push twist 9

[0039] Knob 2 baffle 10

[0040] Cover body buckle 3 comb tooth structure 11

[0041] Box body buckle 4 third side part clamping groove 12

[0042] Bottom clamping groove 5 fourth side part clamping groove 13

[0043] First side part clamping groove 6 first top clamping groove 14

[0044] Second side part clamping groove 7 second top clamping groove 15

[0045] Lower box body 8 DETAILED DESCRIPTION

[0046] The utility model will be helpful for further understanding of the utility model for those skilled in the art with the following embodiments, but it is not in any form limit the utility model.It should be pointed out that for those skilled in the art, on the premise of not departing from the utility model concept, can also make a number of changes and improvements.These all belong to the protection scope of the utility model.

[0047] The utility model discloses a detachable assembly's protein imprinting washing membrane box, adopts the washable membrane box that can splice to not cause the shortage of compartment and waste, also need not to buy multiple specifications' washable membrane box, and need not to discard waste liquid every time in the washing membrane link, can temporarily store waste liquid in lower box body, removes waste liquid after the whole washing membrane ends, can protect experimental personnel and operating environment, also can shorten the time of washing membrane.

[0048] According to the detachable assembly's protein imprinting washing membrane box provided by the utility model, Figure 1 As shown, including upper box body subassembly and lower box body subassembly, the upper box body subassembly includes one upper box body 1 or multiple parallel detachable settings upper box body 1, the lower box body subassembly includes one lower box body 8 or multiple parallel detachable settings lower box body 8, the lower box body 8 corresponds with upper box body 1, and can be detachably installed at the bottom of upper box body 1.

[0049] As Figures 2-6As shown, the upper box body 1 is provided with a cover body at the top, the rear end of the cover body is rotatably connected with the rear end of the upper box body 1 through a knob 2, the front end of the cover body is provided with a cover body buckle 3, and the front end of the upper box body 1 is provided with a box body buckle 4 corresponding to the cover body buckle 3; the upper box body 1 is connected with the box body buckle 4 at the front end of the upper box body 1 through the cover body buckle 3. The top of the upper box body 1 is provided with a first side part clamping groove 6 and a second side part clamping groove 7 on the two sides respectively; in a plurality of parallel upper box bodies 1, the upper box body 1 is connected with the second side part clamping groove 7 of the upper box body 1 on one side through the first side part clamping groove 6, and the upper box body 1 is connected with the first side part clamping groove 6 of the upper box body 1 on the other side through the second side part clamping groove 7.

[0050] As shown, Figures 7-11 The top of the lower box body 8 is provided with a third side part clamping groove 12 and a fourth side part clamping groove 13 on the two sides respectively; in a plurality of parallel lower box bodies 8, the lower box body 8 is connected with the fourth side part clamping groove 13 of the lower box body 8 on one side through the third side part clamping groove 12, and the lower box body 8 is connected with the third side part clamping groove 12 of the lower box body 8 on the other side through the fourth side part clamping groove 13. The compartments of the above-mentioned protein blotting film washing box can be spliced by themselves, corresponding to the number of protein markers applied in each experiment, the splicable film washing box will not cause the shortage of compartments and waste, and it is not necessary to purchase multiple specifications of film washing boxes.

[0051] The bottom of the upper box body 1 is provided with a flow channel for communication with the lower box body, the top of the lower box body is slidably provided with a baffle 10 corresponding to the flow channel, and the baffle 10 is used for opening or closing the flow channel by sliding. The bottom of the upper box body 1 is provided with a bottom clamping groove 5 on the two sides, the top of the lower box body 8 is provided with a first top clamping groove 14 and a second top clamping groove 15 on the two sides respectively; the upper box body 1 is connected with the first top clamping groove 14 and the second top clamping groove 15 of the top of the lower box body 8 through the bottom clamping grooves 5 on the two sides respectively. In the film washing link, the waste liquid can be temporarily stored in the lower box body 8 without being discarded every time, and after the whole film washing is completed, the upper box body 1 and the lower box body 8 are disassembled for waste liquid transfer, which can protect the experimental personnel and the operating environment, and can also shorten the film washing time.

[0052] The top of the baffle 10 is provided with a push and twist 9, and the two sides of the baffle 10 are slidably arranged in the slide ways on the two sides of the top inside the lower box body 8. The rear side of the baffle 10 is provided with a comb tooth structure 11, and the baffle 10 and the comb tooth structure 11 can reciprocally slide between a first position and a second position;

[0053] The first position, in the projection of the top view, the flow channel of the bottom of the upper box body 1 is completely coincident with the baffle 10, and the flow channel is closed by the baffle 10; by opening and closing the baffle 10 between the upper and lower boxes, the membrane washing liquid is temporarily stored in the lower box body from the upper box body, and when the whole membrane washing step is finished, the waste liquid in the lower box body is transferred to the waste liquid barrel at one time;

[0054] The second position, in the projection of the top view, the baffle 10 is located at the front side of the flow channel of the bottom of the upper box body 1, and the baffle 10 is not coincident with the flow channel, the flow channel is opened, and the comb tooth structure 11 is located directly below the flow channel. Through the comb tooth structure 11 at the front end of the baffle 10, on the one hand, the liquid cannot be prevented from transferring to the lower box body 8, and on the other hand, the solid-phase membrane can be prevented from accidentally falling into the lower box body 8.

[0055] In a preferred example, a vertical limiting plate is arranged at the top of the lower box body 8, and a limiting part is arranged at the rear side of the baffle 10, used for limiting the rear side of the vertical limiting plate; when the limiting part is limited with the vertical limiting plate, the baffle 10 and the comb tooth structure 11 are located at the second position. By arranging the vertical limiting baffle, it can be prevented that the baffle 10 is pulled out of the box when moving from the first position to the second position, and it can also be prevented that the comb tooth structure 11 is misaligned with the flow channel, so as to avoid that the solid-phase membrane falls into the lower box body 8. Preferably, a sealing ring is arranged at the top of the baffle 10 along the flow channel of the bottom of the upper box body 1, used for sealing the flow channel.

[0056] In the description of the present application, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application.

[0057] The specific embodiments of the present application are described above. It should be understood that the present application is not limited to the above specific embodiments, and those skilled in the art can make various changes or modifications within the scope of the claims, which does not affect the essential content of the present application. In the case of no conflict, the embodiments of the present application and the features in the embodiments can be combined with each other at will.

Claims

1. A detachably assembled protein blotting washing cassette, characterized by, The upper box body assembly comprises one upper box body (1) or a plurality of upper box bodies (1) arranged side by side and detachably; the lower box body assembly comprises one lower box body (8) or a plurality of lower box bodies (8) arranged side by side and detachably; The lower box body (8) corresponds to the upper box body (1) and is detachably mounted at the bottom of the upper box body (1), the bottom of the upper box body (1) is provided with a flow channel for communication with the lower box body, the top of the lower box body is slidably provided with a baffle (10) corresponding to the flow channel, and the baffle (10) is used for opening or closing the flow channel by sliding.

2. The detachably assembled Western blot membrane washing cassette according to claim 1, wherein, The top of the upper box body (1) is provided with a first side clamping groove (6) and a second side clamping groove (7) on both sides respectively; In the plurality of upper box bodies (1) arranged side by side, the upper box body (1) is connected with the second side clamping groove (7) of the upper box body (1) on one side through the first side clamping groove (6), and the upper box body (1) is connected with the first side clamping groove (6) of the upper box body (1) on the other side through the second side clamping groove (7).

3. The detachably assembled Western blot membrane washing cassette according to claim 1, wherein, The bottom of the upper box body (1) is provided with a bottom clamping groove (5) on both sides, and the top of the lower box body (8) is provided with a first top clamping groove (14) and a second top clamping groove (15) on both sides respectively; The upper box body (1) is connected with the first top clamping groove (14) and the second top clamping groove (15) of the top of the lower box body (8) through the bottom clamping grooves (5) on both sides respectively.

4. The detachably assembled Western blot membrane washing cassette according to claim 1, wherein, The top of the lower box body (8) is provided with a third side clamping groove (12) and a fourth side clamping groove (13) on both sides respectively; In the plurality of lower box bodies (8) arranged side by side, the lower box body (8) is connected with the fourth side clamping groove (13) of the lower box body (8) on one side through the third side clamping groove (12), and the lower box body (8) is connected with the third side clamping groove (12) of the lower box body (8) on the other side through the fourth side clamping groove (13).

5. The detachably assembled Western blot membrane washing cassette according to claim 1, wherein, The top of the upper box body (1) is provided with a cover body, and the rear end of the cover body is rotatably connected with the rear end of the upper box body (1) through a knob (2).

6. The detachably assembled Western blot membrane washing cassette according to claim 5, wherein, The front end of the cover body is provided with a cover body buckle (3), and the front end of the upper box body (1) is provided with a box body buckle (4) corresponding to the cover body buckle (3); The upper box body (1) is connected with the box body buckle (4) at the front end of the upper box body (1) through the cover body buckle (3).

7. The detachably assembled Western blot membrane washing cassette according to claim 1, wherein, The top of the baffle (10) is provided with a push-torque (9), and the baffle (10) is slidably arranged in the slide way on both sides in the top of the lower box body (8) inside.

8. The detachably assembled Western blot membrane washing cassette according to claim 1, wherein, The rear side of the baffle (10) is provided with a comb tooth structure (11), and the baffle (10) and the comb tooth structure (11) can reciprocally slide between a first position and a second position; In the first position, the flow channel at the bottom of the upper box body (1) and the baffle (10) are completely coincident in the projection of the top view, and the flow channel is closed by the baffle (10). The second position is that, in the projection of the top view, the baffle (10) is located at the front side of the bottom flow channel of the upper box body (1), the baffle (10) does not coincide with the flow channel, the flow channel is opened, and the comb tooth structure (11) is located directly below the flow channel.

9. The detachably assembled Western blot membrane washing cassette according to claim 8, wherein, The top of the lower box body (8) is provided with a vertical limiting plate, and the rear side of the baffle (10) is provided with a limiting portion for limiting the rear side of the vertical limiting plate. When the limiting portion is limited by the vertical limiting plate, the baffle (10) and the comb tooth structure (11) are located at the second position.

10. The detachably assembled Western blot membrane washing cassette according to claim 1, wherein, The top of the baffle (10) is provided with a sealing ring along the circumference of the bottom flow channel of the upper box body (1), which is used for sealing the flow channel.

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