Perforating structure of agarose gel plate

Abstract

The utility model relates to the technical field of electrophoresis experiment equipment, in particular to an agarose gel plate punching structure which comprises a base, a containing groove is formed in the top of the base, a supporting frame is fixed to the top of the base, and the supporting frame is located above the containing groove and provided with a bearing plate through a driving piece. A plurality of rows of punching pipes are arranged on the bearing plate, and a variable pitch assembly is arranged between the bearing plate and the punching pipes; wherein the variable-pitch assembly comprises an adjusting screw rod, a fixing piece for preventing mistaken touch is arranged between the adjusting screw rod and the variable-pitch assembly, the adjustment of the hole pitch can optimize the gel layout according to the specific requirements of an experiment, so that the experiment design is more flexible, the size and the pitch of holes can be adjusted according to the volume of a sample, and the size of the holes can be adjusted according to the size of the sample. Each sample can be properly displayed on the gel, the problem caused by too many or too few samples is avoided, and the situation that the adjusting screw rotates due to mistaken touch can be prevented through the fixing piece.

Description

Technical Field

[0001] This utility model relates to the field of electrophoresis experimental equipment technology, and in particular to a perforated structure for agarose gel plates. Background Technology

[0002] Agarose gel electrophoresis uses an electric field to move negatively charged DNA fragments in an agarose gel. Because of the different pore sizes in the gel, smaller DNA fragments pass through the gel more easily than larger fragments, and therefore move faster. By comparing the position of the DNA sample with that of DNA fragments of known size (molecular weight standards), the size of the DNA fragments in the sample can be determined.

[0003] To facilitate drilling holes in the gel plate, an agarose gel plate drilling structure is required. For example, Chinese Patent Publication No. CN216831318U discloses an agarose gel drilling device for detecting influenza hemagglutinin content, comprising three parts: a vacuum unit, a drilling unit, and a loading unit. The vacuum unit includes a vacuum conduit and a vacuum device; one end of the vacuum conduit is connected to the pipe of the drilling cover, and the other end is connected to the vacuum device. The drilling unit includes a drilling disc and a drilling cover, which are fixed together by a nut. The loading unit includes a loading stage and a vertical guide seat; the track hole of the drilling cover fits onto the vertical guide seat of the loading stage, thereby achieving the drilling of vertical and consistent holes in one operation.

[0004] However, in practice, perforated tubes are formed by opening holes in the perforated plate to connect with the cavity. Considering that researchers may need holes with different spacing to optimize experimental conditions or adapt to different sample volumes under different experimental needs, the spacing between multiple perforated tubes cannot be adjusted, which reduces the flexibility and practicality of the equipment. Utility Model Content

[0005] The purpose of this invention is to address the shortcomings of existing technologies where the spacing between multiple perforated tubes cannot be adjusted, and to propose a perforated structure for agarose gel plates.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] Design a perforated structure for agarose gel plate, including a base, a placement groove on the top of the base and a support frame fixed thereon, a receiving plate located above the placement groove and provided by a drive component on the support frame, a plurality of rows of perforated tubes on the receiving plate and a pitch-changing component between the support frame and the perforated tubes;

[0008] The pitch-changing assembly includes an adjusting screw, and a fixing member for preventing accidental contact is provided between the adjusting screw and the pitch-changing assembly.

[0009] Furthermore, the pitch-changing assembly also includes guide seats, which are fixed to both sides of the receiving plate and slidably connected to a sliding plate therebetween. The two ends of one of the guide seats are rotatably connected to the adjusting screw via extension blocks, and the sliding plate is threadedly connected to the adjusting screw via a fixing part.

[0010] Furthermore, several connectors are slidably connected between the guide seats on both sides by a pair of guide rods. The connectors are C-shaped and have two opposite end faces. One end face has a protrusion, and the other end face is located above the slide plate and is fixedly connected to the perforated tube.

[0011] Furthermore, the slide plate has several inclined grooves arranged radially, and the protrusion is movably connected to the inclined grooves.

[0012] Furthermore, the fixing component includes a fixed bracket and a movable bracket. The fixed bracket is fixed to the side of the extension block. The shaft end of the adjusting screw passes through the fixed bracket and is surrounded by the movable bracket. The movable bracket and the fixed bracket are engaged by a groove and a protrusion.

[0013] Furthermore, one end of the adjusting screw has a limiting groove, and a spring is sleeved around it and the movable retainer is slidably connected thereto. The movable retainer abuts against the spring, and a limiting block matching the limiting groove is provided on the inner wall of its through hole.

[0014] The present invention proposes a perforated structure for agarose gel plates, which has the following advantages: The present invention uses a rotating adjusting screw to drive a sliding plate to move, thereby accurately adjusting the spacing between several rows of perforated tubes. The spacing of the holes can be adjusted to optimize the gel layout according to the specific needs of the experiment, making the experimental design more flexible. Furthermore, the size and spacing of the holes can be adjusted according to the volume of the sample, ensuring that each sample can be properly displayed on the gel, avoiding problems caused by too many or too few samples. The fixing component can also prevent accidental rotation of the adjusting screw. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the structure of this utility model;

[0016] Figure 2 This is a schematic diagram of the structure of the perforated tube of this utility model;

[0017] Figure 3 for Figure 2 A magnified structural diagram of area A;

[0018] Figure 4This is a cross-sectional view of the pitch-changing component of this utility model;

[0019] Figure 5 This is a schematic diagram of the structure of the connector of this utility model.

[0020] In the diagram: 1. Base; 11. Placement slot; 2. Support frame; 3. Drive component; 4. Receiving plate; 5. Perforated tube; 6. Pitch conversion assembly; 61. Adjusting screw; 62. Guide seat; 63. Slide plate; 631. Inclined groove; 64. Extension block; 65. Guide rod; 66. Connector; 661. Protrusion; 7. Fixing component; 71. Fixed bracket; 72. Movable bracket; 73. Spring. Detailed Implementation

[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0022] Reference Figure 1-5 A perforated structure for an agarose gel plate includes a base 1. The top of the base 1 has a placement groove 11 and a support frame 2 is fixed thereon. The support frame 2 is located above the placement groove 11 and is provided with a receiving plate 4 via a driving component 3. The receiving plate 4 is provided with several rows of perforated tubes 5 and a pitch-changing component 6 is provided between it and the perforated tubes 5.

[0023] The pitch-changing assembly 6 includes an adjusting screw 61, and a fixing member 7 for preventing accidental contact is provided between the adjusting screw 61 and the pitch-changing assembly 6.

[0024] It should be added that the driving component 3 is a cylinder, which is fixed to the top of the support frame 2 and its shaft end passes through the support frame 2 and is fixedly connected to the receiving plate 4. The cylinder drives the perforating tube 5 to move and perforate the gel plate.

[0025] Furthermore, the pitch-changing assembly 6 also includes a guide seat 62, which is fixed to both sides of the receiving plate 4 and slidably connected to a slide plate 63 therebetween. One end of the guide seat 62 is rotatably connected to the adjusting screw 61 via an extension block 64, and the slide plate 63 is threadedly connected to the adjusting screw 61 via a fixing part.

[0026] Furthermore, a number of connectors 66 are slidably connected between the guide seats 62 on both sides by a pair of guide rods 65. The connectors 66 have a C-shaped structure and have two opposite end faces. One end face is provided with a protrusion 661, and the other end face is located above the slide plate 63 and is fixedly connected to the perforated tube 5.

[0027] More specifically, the slide plate 63 has a plurality of inclined grooves 631 arranged radially, and the protrusion 661 is movably connected to the inclined grooves 631.

[0028] It is worth mentioning that the slide plate 63 moves longitudinally in conjunction with the guide seat 62 under the drive of the adjusting screw 61. At the same time, the slide plate 63 uses the inclined groove 631 in conjunction with the protrusion 661 to drive the connecting member 66 to move accordingly. The connecting member 66 moves laterally because the inclined groove 631 is longitudinally radially opened, thereby achieving the function of adjusting the spacing.

[0029] In general, the fixing member 7 includes a fixed bracket 71 and a movable bracket 72. The fixed bracket 71 is fixed to the side of the extension block 64. The shaft end of the adjusting screw 61 passes through the fixed bracket 71 and the movable bracket 72 is provided on the periphery. The movable bracket 72 and the fixed bracket 71 are engaged by a groove and a protrusion.

[0030] Finally, one end of the adjusting screw 61 has a limiting groove, and a spring 73 is sleeved around it and slidably connected to the movable bracket 72. The movable bracket 72 abuts against the spring 73, and a limiting block matching the limiting groove is provided on the inner wall of its through hole.

[0031] In specific operation, the movable card seat 72 moves along the axis of the adjusting screw 61 under the action of the limiting groove and the limiting block, but cannot rotate at will, thereby ensuring that when the movable card seat 72 is engaged with the fixed card seat 71, it can fix the adjusting screw 61.

[0032] Specifically, the movable bracket 72 has protrusions on both sides, which facilitate pushing the movable bracket 72. Additionally, a handwheel is connected to the end of the adjusting screw 61, and this handwheel abuts against one end of the spring 73.

[0033] It should be added that an air compressor is provided on the top of the receiving plate 4, and several rows of perforated pipes 5 are connected by a first air supply hose. The air compressor is connected to the first air supply hose through a second air supply hose. The air compressor generates negative pressure in the perforated pipes 5 through a pair of air supply hoses to absorb the broken gel blocks.

[0034] Working method: During operation, the gel plate is placed in the placement groove 11, and the distance between the perforated tubes 5 is adjusted according to the required perforation gap;

[0035] First, rotate the adjusting screw 61. Driven by the adjusting screw 61, the slide plate 63 moves longitudinally in conjunction with the guide seat 62. Then, while moving, the slide plate 63 uses the inclined groove 631 and the protrusion 661 to drive the connecting piece 66 to move. The connecting piece 66 moves laterally because the inclined groove 631 is longitudinally radially opened, thus achieving the function of adjusting the spacing.

[0036] The cylinder drives the receiving plate 4 to move the perforated tube 5 downward to perforate the gel plate. The air compressor generates negative pressure in the perforated tube 5 through a pair of air supply hoses to suck up the broken gel pieces. Then the receiving plate 4 moves upward.

[0037] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A perforated structure for an agarose gel plate, comprising a base (1), characterized in that: The base (1) has a placement slot (11) on its top and a support frame (2) fixed thereon. The support frame (2) is located above the placement slot (11) and is provided with a receiving plate (4) via a drive component (3). The receiving plate (4) is provided with several rows of perforated tubes (5) and a pitch-changing component (6) is provided between it and the perforated tubes (5). The pitch-changing assembly (6) includes an adjusting screw (61), and a fixing member (7) for preventing accidental contact is provided between the adjusting screw (61) and the pitch-changing assembly (6).

2. The agarose gel plate perforation structure according to claim 1, characterized in that: The pitch-changing assembly (6) also includes a guide seat (62), which is fixed on both sides of the receiving plate (4) and a sliding plate (63) is slidably connected between them. The two ends of one of the guide seats (62) are rotatably connected to the adjusting screw (61) through an extension block (64), and the sliding plate (63) is threadedly connected to the adjusting screw (61) through a fixing part.

3. The agarose gel plate perforation structure according to claim 2, characterized in that: Several connectors (66) are slidably connected between the guide seats (62) on both sides by a pair of guide rods (65). The connectors (66) are C-shaped and have two opposite end faces. One end face is provided with a protrusion (661), and the other end face is located above the slide plate (63) and is fixedly connected to the perforated tube (5).

4. The agarose gel plate perforation structure according to claim 3, characterized in that: The slide plate (63) has a plurality of inclined grooves (631) arranged radially, and the protrusion (661) is movably connected to the inclined grooves (631).

5. The agarose gel plate perforation structure according to claim 2, characterized in that: The fixing component (7) includes a fixed bracket (71) and a movable bracket (72). The fixed bracket (71) is fixed to the side of the extension block (64). The shaft end of the adjusting screw (61) passes through the fixed bracket (71) and the movable bracket (72) is provided on the periphery. The movable bracket (72) and the fixed bracket (71) are engaged by a groove and a protrusion.

6. The agarose gel plate perforation structure according to claim 5, characterized in that: One end of the adjusting screw (61) has a limiting groove and a spring (73) is sleeved around it. The movable bracket (72) abuts against the spring (73) and a limiting block matching the limiting groove is provided on the inner wall of its through hole.

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