Detachable protein purification plate

Abstract

The utility model relates to the technical field of protein purification, in particular to a detachable protein purification plate which comprises a purification box body, a box door, a coarse separation tank, a fine purification cylinder, a connecting assembly, a feeding assembly and an installation assembly, the installation assembly comprises a guide rail, a guide block, a purification plate body, a push-pull handle and a limiting component, and the guide rail is installed in the purification box body; the guide rail is provided with a guide block, the purification plate body is installed on the guide block, the push-pull handle is installed on the purification plate body, the limiting component is installed on the guide rail, the guide block is aligned to the guide rail, the push-pull handle is pushed to enable the purification plate body to drive the guide block to slide on the guide rail, and the purification plate body is located in the purification box. The guide blocks are limited on the guide rails through the limiting components, so that the purification plate body is stably mounted in the purification box body, the purification plate body is conveniently dismounted and mounted, the experiment complexity is reduced, and the accuracy of an experiment result is improved.

Description

Technical Field

[0001] This utility model relates to the field of protein purification technology, and in particular to a detachable protein purification plate. Background Technology

[0002] Protein purification primarily utilizes the differences between proteins in terms of size, shape, charge, hydrophobicity, solubility, and biological activity to separate them using physical, chemical, or biological methods, thereby obtaining the structure of a specific protein. The protein purification process involves operations such as coarse separation and fine purification to obtain the target protein.

[0003] Existing patent CN213680475U describes a simple protein purification device, comprising a purification chamber. A feeding funnel is fixedly connected to the upper surface of the purification chamber, and a discharge pipe is fixedly connected to the inner top wall of the purification chamber. A coarse separation tank is disposed inside the purification chamber. A flow rate control valve is disposed on the surface of the discharge pipe, and a fine purification cylinder is fixedly connected to the end of the discharge pipe furthest from the feeding funnel. This simple protein purification device, by setting up the feeding funnel, facilitates the addition of the protein to be purified into the purification chamber. The discharge pipe facilitates the delivery of the protein to the coarse separation tank. The coarse separation tank allows for the precipitation and filtration of the protein to be purified, thereby achieving a preliminary purification effect.

[0004] However, when using the existing patented operating device, considering the specific needs of actual experiments, a high-quality, automated purification process for proteins is required, which involves the use of protein purification plates for further separation and purification of proteins. Since the device cannot be disassembled to install the protein purification plates, this increases the complexity of the experiment and affects the accuracy of the experimental results. Utility Model Content

[0005] The purpose of this invention is to provide a detachable protein purification plate, which solves the problem mentioned above. In the process of using the operating device, considering the specific needs of actual experiments, a high-quality and automated protein purification process is required, which will use a protein purification plate for further separation and purification of proteins. However, since the device cannot detach and install the protein purification plate, it increases the complexity of the experiment and affects the accuracy of the experimental results.

[0006] To achieve the above objectives, this utility model provides a detachable protein purification plate, including a purification chamber, a door, a coarse separation tank, a fine purification cylinder, a connecting assembly, a feeding assembly, and an installation assembly. The door is mounted on the purification chamber and is hinged to the purification chamber. The coarse separation tank is fixedly mounted inside the purification chamber. The fine purification cylinder is mounted on the coarse separation tank via the connecting assembly. The feeding assembly is mounted on the purification chamber. The installation assembly includes guide rails, guide blocks, a purification plate body, a push-pull handle, and a limiting component. There are two guide rails, each mounted inside the purification chamber. Each guide rail is equipped with a guide block, which is slidably connected to the guide rail. The purification plate body is fixedly mounted on the guide block. The push-pull handle is fixedly mounted on the purification plate body. The limiting component is mounted on the guide rail.

[0007] The limiting component includes a limiting rod and a nut. The limiting rod is installed on the guide rail, passes through the guide rail, and is located inside the guide block. The nut is installed on the limiting rod and is threadedly connected to the limiting rod.

[0008] The connection assembly includes a connecting pipe and a control valve. The connecting pipe is fixedly installed on the coarse separation tank and communicates with the coarse separation tank. The control valve is installed on the connecting pipe. The fine purification cylinder is fixedly installed on the connecting pipe and communicates with the connecting pipe.

[0009] The feeding assembly includes a discharge pipe, a feeding funnel, and a discharge component. The discharge pipe is fixedly installed on the purification chamber and is connected to the coarse separation tank. The feeding funnel is fixedly installed on the discharge pipe and is connected to the discharge pipe. The discharge component is installed inside the purification chamber.

[0010] The discharge component includes a support plate, a collection funnel, and a collection container. The support plate is fixedly installed inside the purification chamber, the collection funnel is fixedly installed on the support plate, and the collection container is installed inside the purification chamber.

[0011] This utility model discloses a detachable protein purification plate. The guide rail is fixedly installed inside the purification chamber. The guide block is installed on the guide rail, the purification plate body is fixedly installed on the guide block, and the push-pull handle is fixedly installed on the purification plate body. In use, the guide block is aligned with the guide rail, and the push-pull handle is pushed to cause the purification plate body to slide along the guide rail, positioning the purification plate body inside the purification chamber and abutting against it. Then, the limiting rod is used to pass through the guide rail and is positioned inside the guide block, thus limiting the guide block on the guide rail. Finally, the plate is rotated... The nut is used to fix the limiting rod. Then, the protein is poured into the purification chamber through the feeding funnel and enters the coarse separation tank through the discharge pipe for coarse separation. Then, the control valve is opened to allow the target protein to flow into the fine purification cylinder through the connecting pipe for separation. Then, it flows into the purification plate body for further separation and finally flows into the collection container through the collection funnel to collect the target protein. This facilitates the disassembly and installation of the purification plate body, enabling high-quality and automated purification of proteins, reducing experimental complexity and improving the accuracy of experimental results. Attached Figure Description

[0012] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below.

[0013] Figure 1 This is a schematic diagram of the overall structure of the detachable protein purification plate of this utility model.

[0014] Figure 2 This is a three-dimensional drawing of the detachable protein purification plate of this utility model.

[0015] Figure 3 This is a front view of the detachable protein purification plate of this utility model.

[0016] In the diagram: 101-Purification chamber, 102-Door, 103-Crude separation tank, 104-Fine purification cylinder, 105-Guide rail, 106-Guide block, 107-Purification plate body, 108-Push-pull handle, 109-Limit rod, 110-Nut, 111-Connecting pipe, 112-Control valve, 113-Discharge pipe, 114-Feeding funnel, 115-Support plate, 116-Collection funnel, 117-Collection container. Detailed Implementation

[0017] The embodiments of the present invention are described in detail below. Examples of the embodiments are shown in the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain the present invention, but should not be construed as limiting the present invention.

[0018] Please see Figures 1 to 3 ,in Figure 1 This is a schematic diagram of the overall structure of the detachable protein purification plate of this utility model; Figure 2 This is a three-dimensional view of the detachable protein purification plate of this utility model; Figure 3 This is a front view of the detachable protein purification plate of this utility model.

[0019] This utility model provides a detachable protein purification plate, comprising a purification chamber 101, a door 102, a coarse separation tank 103, a fine purification cylinder 104, a connecting assembly, a feeding assembly, and an installation assembly. The installation assembly includes a guide rail 105, a guide block 106, a purification plate body 107, a push-pull handle 108, and a limiting component. The limiting component includes a limiting rod 109 and a nut 110. The connecting assembly includes a connecting pipe 111 and a control valve 112. The feeding assembly includes a discharge pipe 113, a feeding funnel 114, and a discharge component. The discharge component includes a support plate 115, a collection funnel 116, and a collection container 117. This solution addresses the problem that, in the process of using the operating device, considering the specific needs of actual experiments, a high-quality, automated protein purification process is required, which involves the use of a protein purification plate for further protein separation and purification. Because the device cannot detach and install the protein purification plate, this increases experimental complexity and affects the accuracy of experimental results. Therefore, this solution can be used in protein purification scenarios.

[0020] In this embodiment, the door 102 is mounted on the purification chamber 101 and is hinged to the purification chamber 101. The coarse separation tank 103 is fixedly mounted inside the purification chamber 101. The fine purification tube 104 is mounted on the coarse separation tank 103 via the connecting assembly. The feeding assembly is mounted on the purification chamber 101. The mounting assembly is mounted inside the purification chamber 101, thereby enabling high-quality and automated purification of proteins, reducing experimental complexity and improving the accuracy of experimental results. The door 102 allows easy access to the purification chamber 101 for maintenance and disassembly. The coarse separation tank 103 utilizes isoelectric point precipitation to initially separate proteins based on differences in solubility under different conditions. This coarse separation tank 103 is a mature existing technology and will not be described further in this solution. The fine purification tube 104 uses gel filtration to further purify the crude product. This fine purification tube 104 is also a mature existing technology and will not be described further in this solution. The connecting assembly connects the coarse separation tank 103 and the fine purification tube 104. The feeding assembly facilitates the entry of proteins into the purification chamber 101.

[0021] The number of guide rails 105 is two, and the two guide rails 105 are respectively installed in the purification chamber 101. Each guide rail 105 is equipped with a guide block 106, and the guide block 106 is slidably connected to the guide rail 105. The purification plate body 107 is fixedly installed on the guide block 106, the push-pull handle 108 is fixedly installed on the purification plate body 107, and the limiting member is installed on the guide rail 105. Two guide rails 105 are located on the left and right side walls of the purification chamber 101, respectively. The guide rails 105 support the guide block 106, allowing the guide block 106 to slide on the guide rails 105. The guide block 106 supports the purification plate body 107, allowing the purification plate body 107 to be installed inside the purification chamber 101. The purification plate body 107 is filled with affinity chromatography packing material and ion exchange resin for further separation and purification of finely purified proteins. It can be used for high-quality, automated purification processes. The purification plate body 107 is a mature existing technology and will not be described further in this solution. The push-pull handle 108 facilitates pushing and pulling the purification plate body 107 for disassembly and installation. The limiting member limits the guide block 106 to the guide rail 105. In use, the guide block 106 is aligned with the guide rail 105. 05. Pushing the push-pull handle 108 causes the purification plate body 107 to slide along the guide block 106 on the guide rail 105, positioning the purification plate body 107 within the purification chamber 101 and abutting against it. The limiting member then limits the guide block 106 to the guide rail 105, preventing the purification plate body 107 from moving under external force and ensuring its stable installation within the purification chamber 101. Protein is then poured into the purification chamber 101 through the feeding assembly, undergoes coarse separation in the coarse separation tank 103, flows through the connecting assembly into the fine purification cylinder 104 for further separation, and then flows back onto the purification plate body 107 for further separation. This facilitates the disassembly and installation of the purification plate body 107, enabling high-quality, automated protein purification, reducing experimental complexity, and improving the accuracy of experimental results.

[0022] Secondly, the limiting rod 109 is installed on the guide rail 105 and passes through the guide rail 105, and is located inside the guide block 106. The nut 110 is installed on the limiting rod 109 and is threadedly connected to the limiting rod 109. Each guide rail 105 is equipped with a limiting rod 109. The guide rail 105 has a through hole, and the guide block 106 has a limiting groove. The limiting rod 109 passes through the through hole and is located in the limiting groove, so that the guide block 106 is limited on the guide rail 105. The nut 110 is threaded onto the limiting rod 109. Rotating the nut 110 causes the nut 110 to move on the limiting rod 109 and abut against the guide rail 105, so that the limiting rod 109 is fixed on the guide rail 105. The limiting rod 109 passes through the guide rail 105 and is located in the guide block 106. Rotating the nut 110 again fixes the limiting rod 109 on the guide rail 105, so that the guide block 106 is fixed on the guide rail 105, and the purification plate body 107 is not moved by external force.

[0023] Next, the connecting tube 111 is fixedly installed on the coarse separation tank 103, and the connecting tube 111 is connected to the coarse separation tank 103. The control valve 112 is installed on the connecting tube 111, and the fine purification cylinder 104 is fixedly installed on the connecting tube 111, and the fine purification cylinder 104 is connected to the connecting tube 111. The connecting tube 111 supports the fine purification cylinder 104, and the connecting tube 111 connects the coarse separation tank 103 and the fine purification cylinder 104, allowing the coarsely separated protein to flow through the connecting tube 111 into the fine purification cylinder 104 for purification. The control valve 112 controls the opening and closing of the connecting tube 111. After the protein undergoes coarse separation in the coarse separation tank 103, the control valve 112 is opened, allowing the target protein to flow through the connecting tube 111 into the fine purification cylinder 104, thereby undergoing purification and separation.

[0024] The feed pipe 113 is fixedly installed on the purification chamber 101 and is connected to the coarse separation tank 103. The feeding funnel 114 is fixedly installed on the feed pipe 113 and is connected to the feed pipe 113. The discharge component is installed inside the purification chamber 101. The feed pipe 113 connects the coarse separation tank 103 and the feeding funnel 114 to transport protein raw materials. The feeding funnel 114 facilitates the pouring of protein raw materials into the purification chamber 101. The discharge component collects the purified target protein. The protein raw materials are poured into the purification chamber 101 through the feeding funnel 114 and enter the coarse separation tank 103 through the feed pipe 113, thus facilitating feeding.

[0025] Then, the support plate 115 is fixedly installed inside the purification chamber 101, the collection funnel 116 is fixedly installed on the support plate 115, and the collection container 117 is installed inside the purification chamber 101. The support plate 115 is installed inside the purification chamber 101 by welding, and the support plate 115 supports the collection funnel 116; the collection funnel 116 collects the protein purified by the purification plate body 107, and then transports it to the collection container 117; the collection container 117 collects the target protein.

[0026] In this embodiment, when using the detachable protein purification plate, the guide block 106 is aligned with the guide rail 105. The push-pull handle 108 is pushed, causing the purification plate body 107 to slide along the guide block 106 on the guide rail 105, positioning the purification plate body 107 within the purification chamber 101 and abutting against it. Then, the limiting rod 109 is used to pass through the guide rail 105 and is positioned within the guide block 106, thus limiting the guide block 106 to the guide rail 105. The nut 110 is then rotated to secure the limiting rod 109. Finally, the protein is fed through the feeding funnel 1. 14 is poured into the purification chamber 101 and enters the coarse separation tank 103 through the feed pipe 113 for coarse separation. Then, the control valve 112 is opened to allow the target protein to flow through the connecting pipe 111 into the fine purification cylinder 104 for separation. The protein then flows onto the purification plate body 107 for further separation and finally flows through the collection funnel 116 into the collection container 117 to collect the target protein. This facilitates the disassembly and installation of the purification plate body 107, enabling high-quality and automated purification of the protein, reducing experimental complexity and improving the accuracy of experimental results.

[0027] The above-disclosed embodiments are merely one or more preferred embodiments of this application and should not be construed as limiting the scope of this application. Those skilled in the art can understand that all or part of the processes for implementing the above embodiments and equivalent changes made in accordance with the claims of this application still fall within the scope of this application.

Claims

1. A detachable protein purification plate, comprising a purification chamber, a door, a coarse separation tank, a fine purification cylinder, a connecting assembly, and a feeding assembly, wherein the door is mounted on the purification chamber and hinged to the purification chamber; the coarse separation tank is fixedly mounted inside the purification chamber; the fine purification cylinder is mounted on the coarse separation tank via the connecting assembly; and the feeding assembly is mounted on the purification chamber, characterized in that... It also includes installation components; The installation assembly includes a guide rail, a guide block, a purification plate body, a push-pull handle, and a limiting component. There are two guide rails, each installed inside the purification chamber. A guide block is mounted on each guide rail and is slidably connected to the guide rail. The purification plate body is fixedly mounted on the guide block. The push-pull handle is fixedly mounted on the purification plate body. The limiting component is mounted on the guide rail. The limiting component includes a limiting rod and a nut. The limiting rod is mounted on the guide rail, passes through the guide rail, and is located within the guide block. The nut is mounted on the limiting rod and is threadedly connected to the limiting rod. The connection assembly includes a connecting pipe and a control valve. The connecting pipe is fixedly mounted on the coarse separation tank and communicates with it. The control valve is mounted on the connecting pipe. The fine purification cylinder is fixedly mounted on the connecting pipe and communicates with it.

2. The detachable protein purification plate as described in claim 1, characterized in that, The feeding assembly includes a discharge pipe, a feeding funnel, and a discharge component. The discharge pipe is fixedly installed on the purification chamber and is connected to the coarse separation tank. The feeding funnel is fixedly installed on the discharge pipe and is connected to the discharge pipe. The discharge component is installed inside the purification chamber.

3. The detachable protein purification plate as described in claim 2, characterized in that, The discharge component includes a support plate, a collection funnel, and a collection container. The support plate is fixedly installed inside the purification chamber, the collection funnel is fixedly installed on the support plate, and the collection container is installed inside the purification chamber.

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