Cryopreserved stem cell culture device

Abstract

The utility model relates to the technical field of cell cryopreservation, in particular to a cryopreserved stem cell culture device which comprises a water bath kettle body, a driving mechanism and a placing mechanism, a support frame is detachably mounted at the top end of the water bath kettle body; the driving mechanism is arranged on the supporting frame, the placing frame is installed on the driving mechanism, and the placing frame is driven by the driving mechanism to reciprocate left and right in the horizontal direction along the supporting frame; the placing mechanism is detachably installed on the placing frame, the placing mechanism is composed of a placing disc and a supporting seat installed at the bottom end of the placing disc, a plurality of sets of placing holes are evenly formed in the placing disc, and clamping assemblies used for installing stem cell cryopreservation tubes are arranged in the placing holes. Through the arrangement of the placing mechanism, a plurality of groups of stem cell cryopreservation tubes can be installed, the placing mechanism and the placing frame are convenient and fast to disassemble and assemble, the driving mechanism can drive the plurality of groups of stem cell cryopreservation tubes on the placing mechanism at one time to simultaneously perform uniform oscillation water bath, unfreezing is accelerated, and the culture efficiency is improved.

Description

Technical Field

[0001] This utility model relates to the field of cell cryopreservation technology, specifically to a cryopreservation stem cell culture device. Background Technology

[0002] Cell cryopreservation is a technique that places cells in a low-temperature environment to reduce cell metabolism and allow for long-term storage. When needed, cells frozen in liquid nitrogen are thawed and recultured. When the cells return to room temperature, their morphology and structure are normal and they can be used for experiments. Currently, frozen stem cells need to be thawed before culturing.

[0003] Chinese Patent No. CN221940511U discloses a cryopreservation stem cell culture device. This device, through the combined use of a shaking mechanism and an adjustment mechanism, not only automatically shakes the stem cell cryopreservation tubes in a water bath, ensuring even heating and avoiding uneven melting that can damage stem cells during manual operation, but also adjusts the depth of the cryopreservation tubes submerged in water. This prevents water from overflowing the cryopreservation tube caps and entering the tubes, thus contaminating the stem cells, further facilitating the reculturing of stem cells.

[0004] However, the above-mentioned publicly available solutions have the following shortcomings: Although the shaking mechanism can make the stem cell cryopreservation tubes shake in the water bath and make the stem cell cryopreservation tubes heat evenly, only one stem cell cryopreservation tube can be thawed at a time, which reduces the efficiency of stem cell culture. Utility Model Content

[0005] The purpose of this invention is to address the problems existing in the background technology by proposing a cryopreservation stem cell culture device.

[0006] The technical solution of this utility model is as follows: a cryopreservation stem cell culture device, including a water bath body with a support frame detachably mounted on its top; a drive mechanism disposed on the support frame, a placement frame mounted on the drive mechanism, the placement frame being driven by the drive mechanism to move horizontally back and forth along the support frame; and a placement mechanism detachably mounted on the placement frame, the placement mechanism consisting of a placement tray and a support base mounted on the bottom of the placement tray, the placement tray being detachably connected to the placement frame, the placement tray having multiple sets of placement holes evenly distributed on it, and clamping components for installing stem cell cryopreservation tubes being disposed inside the placement holes.

[0007] Preferably, the support frame includes a top seat with two sets of adjusting rods symmetrically arranged on it, and a socket installed at the bottom of the adjusting rod; and a clamping plate with a socket slidably connected to its top, and a screw threadedly connected to one side wall of the socket, with one end of the screw rotatably connected to the clamping plate.

[0008] Preferably, both sets of adjusting rods are slidably connected to the mounting groove at the top of the top seat, and a double-acting screw is threaded between the two sets of adjusting rods, with a dial wheel installed in the middle of the double-acting screw.

[0009] Preferably, the placement rack consists of a U-shaped plate and a base installed at its bottom. The U-shaped plate is mounted on a drive mechanism and is driven by the drive mechanism to move back and forth horizontally along the support frame. Two sets of insertion holes are symmetrically opened on the base, and the placement plate is slidably connected to the inner wall of the base.

[0010] Preferably, two sets of sliding grooves are symmetrically opened on the placement tray, and an L-shaped slider is slidably connected inside the sliding groove. A second spring is installed between the side wall of the L-shaped slider and the sliding groove, and the insertion end of the L-shaped slider is slidably connected to the insertion hole.

[0011] Preferably, the clamping assembly consists of multiple sets of abutment plates and multiple sets of elastic elements evenly installed on the inner wall of the placement hole. The other end of the elastic element is connected to the abutment plate, and the top of the abutment plate is provided with an inclined portion.

[0012] Compared with the prior art, the above-mentioned technical solution of this utility model has the following beneficial technical effects: This utility model can install multiple sets of stem cell cryopreservation tubes through the placement mechanism, and the placement mechanism and placement rack are easy to disassemble and assemble. Through the drive mechanism, multiple sets of stem cell cryopreservation tubes on the placement rack can be driven at one time to perform uniform shaking water bath, which accelerates thawing and improves culture efficiency. Through the setting of the support frame, the placement rack can be driven to be installed on water bath bodies of different sizes, and the support frame can be adjusted in height along the water bath body, which makes it easy to adjust the water bath depth of the stem cell cryopreservation tubes on the placement rack relative to the water in the water bath body, making it more flexible to use. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0014] Figure 2 This is a schematic diagram of the support frame structure of this utility model;

[0015] Figure 3 This is a bottom view showing the connection between the drive mechanism and the support frame of this utility model;

[0016] Figure 4 This is a schematic diagram of the placement mechanism and placement rack installation method of this utility model.

[0017] Reference numerals in the attached drawings: 1. Water bath body; 2. Top seat; 201. Adjusting rod; 202. Two-way lead screw; 203. Socket; 204. Clamping plate; 205. Screw; 3. Turntable; 301. Slider; 302. Transmission rod; 303. U-shaped plate; 304. Base; 305. Insertion hole; 4. Placement tray; 401. Placement hole; 402. Elastic element; 403. Abutment plate; 404. L-shaped slider; 405. Second spring; 406. Support base. Detailed Implementation

[0018] Example 1

[0019] like Figures 1 to 4 As shown, this utility model proposes a cryopreservation stem cell culture device, including a water bath body 1, a drive mechanism, and a placement mechanism. A support frame is detachably installed on the top of the water bath body 1. The drive mechanism is located on the bottom of the top seat 2 in the support frame, and a placement frame is installed on the drive mechanism. The placement frame is driven by the drive mechanism to move horizontally back and forth along the support frame. The drive mechanism includes a turntable 3, a slider 301, and a transmission rod 302. The turntable 3 is driven to rotate by a motor installed at the bottom of the top seat 2. The slider 301 is slidably connected to the sliding cavity at the bottom of the top seat 2. A connecting rod is installed at the bottom of the slider 301. The two ends of the transmission rod 302 are respectively connected to the connecting rod and the turntable. The bottom edge of the 3 is rotatably connected, and the turntable 3 is driven to rotate by the motor. Then, the transmission rod 302 drives the slider 301 to move back and forth along the slide cavity, which in turn drives the placement rack to move back and forth along the support frame. This causes the cryopreservation tube to vibrate within a suitable range inside the water bath body 1, which can make the stem cell cryopreservation tube heat more evenly and accelerate the thawing. The placement mechanism is detachably installed on the placement rack. The placement mechanism consists of a placement plate 4 and a support base 406 installed at the bottom of the placement plate 4. The placement plate 4 is detachably connected to the placement rack. Multiple sets of placement holes 401 are evenly opened on the placement plate 4. The placement holes 401 are equipped with clamping components for installing stem cell cryopreservation tubes.

[0020] Furthermore, the support frame includes a top seat 2 and a clamping plate 204; two sets of adjusting rods 201 are symmetrically arranged on the top seat 2, and a socket 203 is installed at the bottom of the adjusting rod 201. The socket 203 is U-shaped, and a scale is provided on the inner wall of the socket 203 to facilitate the up-and-down movement and adjustment of the socket 203 along the side wall of the water bath body 1, thereby adjusting the depth of the cryopreservation tube placed on the support frame in the water bath body 1; the top of the clamping plate 204 is slidably connected to the socket 203, and the top of the socket 203 is provided with a limiting slide that matches the clamping plate 204, so that the clamping plate 204 always slides and adjusts along the horizontal direction of the socket 203. A screw 205 is threadedly connected to one side wall of the socket 203, and one end of the screw 205 is rotatably connected to the clamping plate 204.

[0021] Furthermore, the placement frame consists of a U-shaped plate 303 and a base 304 installed at its bottom. The U-shaped plate 303 is mounted on a drive mechanism and is driven by the drive mechanism to move back and forth horizontally along the top seat 2 in the support frame. The top of the U-shaped plate 303 is connected to the connecting rod at the bottom of the slider 301. The base 304 is annular and has two sets of symmetrical insertion holes 305. The placement plate 4 is slidably connected to the inner wall of the base 304.

[0022] Furthermore, two sets of sliding grooves are symmetrically provided on the placement plate 4. An L-shaped slider 404 is slidably connected inside the sliding groove. A second spring 405 is installed between the side wall of the L-shaped slider 404 and the sliding groove. The insertion end of the L-shaped slider 404 is slidably connected to the insertion hole 305.

[0023] Furthermore, the clamping assembly consists of multiple sets of abutment plates 403 and multiple sets of elastic elements 402 evenly installed on the inner wall of the placement hole 401. The other end of the elastic element 402 is connected to the abutment plate 403. The elastic element 402 consists of a first spring and a telescopic rod sleeved on its outside. The top of the abutment plate 403 is provided with an inclined part to facilitate the sliding installation of the stem cell cryopreservation tube.

[0024] In this embodiment, two sets of sockets 203 are respectively clamped on both sides of the top of the water bath body 1. Then, the corresponding screws 205 are rotated to drive the clamping plate 204 to slide along the top of the socket 203 until the clamping plate 204 and the socket 203 clamp the side wall of the water bath body 1, thereby completing the stable installation of the support frame and the water bath body 1.

[0025] Place the support base 406 on the table surface. Slide the stem cell cryopreservation tube between the multiple sets of abutment plates 403 in the placement hole 401 until it reaches the appropriate position, until the top of the stem cell cryopreservation tube abuts the top of the abutment plate 403. Under the elastic force of the elastic element 402, the corresponding abutment plates 403 clamp the stem cell cryopreservation tube from different directions. Install the other sets of stem cell cryopreservation tubes in the corresponding placement holes 401 in sequence. Then slide the two sets of L-shaped sliders 404 closer to each other along the corresponding grooves, so that the second spring 405 is compressed. Then slide the support base 406 into the base 304 and release the L-shaped sliders 404. The restraint of the second spring 405, under the elastic recovery action, drives the L-shaped slider 404 to connect with the insertion hole 305 on the base 304, thereby limiting the placement tray 4 on the support frame. At this time, the placement tray 4 drives multiple sets of stem cell cryopreservation tubes into the water bath body 1. By controlling the water level in the water bath body 1, the multiple sets of stem cell cryopreservation tubes on the placement frame can be driven to vibrate at one time through the drive mechanism. This can make the stem cell cryopreservation tubes heat more evenly, accelerate thawing, and improve culture efficiency. After thawing for a certain period of time, the L-shaped sliders 404 of the connecting trays are brought closer together, and the placement tray 4 can be separated from the placement frame, making it more convenient to use.

[0026] Example 2

[0027] like Figure 1 and Figure 2 As shown, the cryopreservation stem cell culture device proposed in this utility model, compared with Embodiment 1, has two sets of adjusting rods 201 that are slidably connected to the mounting groove at the top of the top seat 2, and a bidirectional lead screw 202 is threaded between the two sets of adjusting rods 201, with a dial wheel installed in the middle of the bidirectional lead screw 202.

[0028] In this embodiment, rotating the dial drives the bidirectional lead screw 202 to rotate. Since the adjusting rod 201 is slidably connected to the mounting groove, it drives the two sets of adjusting rods 201 to move away from or closer to each other, thereby adjusting the distance between the two sets of sockets 203. This makes it easier for the support frame to adapt to the installation of water bath bodies 1 of different sizes, making it more flexible to use.

[0029] The embodiments of the present invention have been described in detail above with reference to the accompanying drawings. However, the present invention is not limited thereto. Various changes can be made within the scope of knowledge possessed by those skilled in the art without departing from the spirit of the present invention.

Claims

1. A cryopreservation stem cell culture device, characterized in that, include The water bath body (1) has a detachable support frame installed on its top; The drive mechanism is mounted on the support frame, and the placement frame is installed on the drive mechanism. The placement frame is driven by the drive mechanism to move horizontally back and forth along the support frame. The placement mechanism is detachably mounted on the placement rack. The placement mechanism consists of a placement tray (4) and a support base (406) mounted on the bottom of the placement tray (4). The placement tray (4) is detachably connected to the placement rack. Multiple placement holes (401) are evenly opened on the placement tray (4). Clamping components for installing stem cell cryopreservation tubes are provided inside the placement holes (401).

2. The cryopreservation stem cell culture device according to claim 1, characterized in that, Support frame includes The top seat (2) has two sets of adjusting rods (201) symmetrically arranged on it, and the bottom end of the adjusting rods (201) is equipped with a socket (203); And a clamping plate (204), the top of which is slidably connected to a socket (203), and a screw (205) is threadedly connected to one side wall of the socket (203), one end of which is rotatably connected to the clamping plate (204).

3. The cryopreservation stem cell culture device according to claim 2, characterized in that, Both sets of adjusting rods (201) are slidably connected to the mounting groove at the top of the top seat (2), and a double-acting screw (202) is threaded between the two sets of adjusting rods (201). A dial is installed in the middle of the double-acting screw (202).

4. The cryopreservation stem cell culture device according to claim 1, characterized in that, The placement rack consists of a U-shaped plate (303) and a base (304) installed at its bottom. The U-shaped plate (303) is mounted on a drive mechanism. The U-shaped plate (303) is driven by the drive mechanism to move back and forth horizontally along the support frame. Two sets of insertion holes (305) are symmetrically opened on the base (304). The placement plate (4) is slidably connected to the inner wall of the base (304).

5. The cryopreservation stem cell culture device according to claim 4, characterized in that, Two sets of sliding grooves are symmetrically opened on the placement plate (4). An L-shaped slider (404) is slidably connected inside the sliding groove. A second spring (405) is installed between the side wall of the L-shaped slider (404) and the sliding groove. The insertion end of the L-shaped slider (404) is slidably connected to the insertion hole (305).

6. The cryopreservation stem cell culture device according to claim 1, characterized in that, The clamping assembly consists of multiple sets of abutment plates (403) and multiple sets of elastic elements (402) evenly installed on the inner wall of the placement hole (401). The other end of the elastic element (402) is connected to the abutment plate (403), and the top of the abutment plate (403) is provided with an inclined part.

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