Sample detection box and sample detection equipment

Abstract

The utility model discloses a sample detection box and sample detection equipment, the sample detection box comprises a detection tube, a housing and a bottom box, and the housing is provided with a liquid relief hole; the bottom box is integrally formed and can be arranged in the housing in a relatively rotating manner, a placing groove is formed in the bottom box, a liquid storage area is formed in the bottom box, a plurality of placing holes for placing the detection tubes are formed in the bottom wall of the placing groove, and the liquid storage area and the plurality of placing holes are annularly arranged at intervals and can sequentially rotate to be communicated with the pipetting hole. Each placing hole can be used for placing one detection tube, so that the same detection reagent or different detection reagents can be respectively stored in the plurality of detection tubes, the same detection index can be detected for multiple times at one time, the detection efficiency is greatly improved, or a plurality of detection indexes can be detected at one time; and synchronous detection of various pathogens is realized.

Description

Technical Field

[0001] This utility model belongs to the field of sample detection technology, specifically relating to a sample detection box and sample detection equipment. Background Technology

[0002] Point-of-care testing (POCT) refers to a testing method that performs analysis immediately at the sampling site, eliminating the complex processing procedures of laboratory testing and providing rapid results. Currently, nucleic acid testing involves placing collected samples into a test kit for pretreatment, and then adding the pretreated samples to test reagents for pathogen detection. This process is cumbersome, prone to introducing impurities, requires specialized personnel who cannot leave the laboratory environment, hindering the realization of point-of-care nucleic acid testing. Furthermore, each test kit can only detect one pathogen once, resulting in limited detection indicators and low efficiency. Utility Model Content

[0003] In view of the above-mentioned defects or deficiencies, this utility model provides a sample detection box and sample detection equipment, aiming to solve the technical problems of existing detection boxes having single detection indicators and low detection efficiency.

[0004] To achieve the above objectives, this utility model provides a sample detection box, which includes:

[0005] Detection tube;

[0006] The housing has a pipetting port.

[0007] The bottom box is integrally formed and can be rotatably disposed inside the cover. The bottom box has a placement groove and a liquid storage area. The bottom wall of the placement groove has multiple placement holes for placing the test tube. The liquid storage area and the multiple placement holes are arranged in a circumferential interval and can be rotated in sequence to communicate with the pipetting hole.

[0008] In this embodiment of the utility model, the bottom box includes a panel and a surrounding panel. The surrounding panel surrounds the periphery of the panel, and a placement groove is formed on the side of the panel facing the pipetting hole, forming a liquid storage area.

[0009] In this embodiment of the utility model, the bottom box also includes a plurality of limiting sleeves, which are disposed on the side of the panel opposite to the pipetting hole, and the plurality of limiting sleeves are respectively connected to the plurality of placement holes one by one.

[0010] In this embodiment of the utility model, the bottom box also includes a plurality of reinforcing plates, which are spaced apart on the side of the panel facing away from the pipetting hole, and the limiting sleeve is located between the surrounding plate and the plurality of reinforcing plates.

[0011] In this embodiment of the utility model, a connection hole is provided on the panel, and a placement groove and a liquid storage area are arranged around the outer periphery of the connection hole. The sample detection box also includes a connecting pin, one end of which can be rotatably passed through the connection hole and connected to the panel, and the other end of which is connected to the cover.

[0012] In this embodiment of the utility model, a positioning groove is provided on the side of the cover facing away from the bottom box. The connecting pin includes a pin body, a limiting block and a positioning piece. The limiting block and the positioning piece are respectively provided at both ends of the pin body. The pin body passes through the bottom wall of the positioning groove and the connecting hole. The limiting block abuts against the side of the panel facing away from the pipetting hole. The positioning piece is located in the positioning groove.

[0013] In this embodiment of the utility model, a sample tank, a liquid storage tank, and a pretreatment tank are provided in the liquid storage area. The sample tank, the liquid storage tank, and the pretreatment tank are arranged at intervals and can be rotated in sequence to communicate with the pipetting hole.

[0014] In this embodiment of the utility model, a storage groove is also provided in the liquid storage area, and a pick-up and put-out hole is provided on the cover corresponding to the position of the storage groove. The sample detection box also includes a pipette tip, which passes through the pick-up and put-out hole and extends into the storage groove.

[0015] In this embodiment of the invention, the sample detection box further includes a sealing cap that covers the pipette opening;

[0016] And / or, the housing is also provided with positioning holes spaced apart from the pipetting holes.

[0017] To achieve the above objectives, the present invention also provides a sample detection device, which includes a sample detection box as described above.

[0018] Through the above technical solutions, the sample detection box and sample detection device provided by the present invention have the following beneficial effects:

[0019] In the technical solution of this utility model, during sample testing, the cover rotates relative to the bottom box to align the pipetting orifice with the storage area, thereby enabling pretreatment of the sample in the storage area through the pipetting orifice. After sample pretreatment, the cover rotates relative to the bottom box to align the pipetting orifice with multiple placement holes in sequence, allowing the pretreated sample to be injected into multiple detection tubes in sequence. The multiple detection tubes can each contain the same detection reagent or different detection reagents. The sample detection box is then installed into the sample detection device, enabling multiple tests on the same detection index at once, significantly improving detection efficiency. Alternatively, multiple detection indicators can be tested simultaneously, achieving simultaneous detection of multiple pathogens. Furthermore, the bottom box integrates the storage area and multiple placement holes, exhibiting high structural integration and portability. The bottom box is a one-piece molded component, offering advantages such as ease of manufacturing, reduced assembly steps, and minimized assembly errors. The cover protects the bottom box from the introduction of impurities, making it suitable for operation in outdoor environments. The sample detection box has a wide range of applications and facilitates real-time sample detection.

[0020] Other features and advantages of this invention will be described in detail in the following detailed description section. Attached Figure Description

[0021] The accompanying drawings are provided to further illustrate the embodiments of the present invention and form part of the specification. They are used together with the following detailed description to explain the embodiments of the present invention, but do not constitute a limitation thereof. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without any inventive effort. In the drawings:

[0022] Figure 1 This is a schematic diagram of the assembly structure of a sample detection box according to an embodiment of the present invention;

[0023] Figure 2 This is an exploded structural diagram of a sample detection box according to an embodiment of the present invention;

[0024] Figure 3 This is an exploded structural diagram of the detection tube and the bottom box in a sample detection box according to an embodiment of the present invention;

[0025] Figure 4 This is a schematic diagram of the structure of the bottom box in a sample testing box according to an embodiment of the present invention;

[0026] Figure 5 This is a schematic diagram of the structure of the cover in the sample detection box according to an embodiment of the present invention;

[0027] Figure 6 This is a schematic diagram of the connecting pin in a sample detection box according to an embodiment of the present invention.

[0028] Explanation of reference numerals in the attached figures

[0029] 10 Detection tubes 324 storage slot

[0030] 11 Sealing film 325 Protease tank

[0031] 12 freeze-dried balls 33 panel

[0032] 20. Housing 332. Connection hole

[0033] 21 Pipetting orifice 333 Connecting sleeve

[0034] 22 Positioning groove 34 Enclosure panel

[0035] 23 Removal / Placement Hole 341 Groove

[0036] 24 Positioning hole 35 Limiting sleeve

[0037] 25 Positioning protrusions 36 Reinforcing plates

[0038] 30 Base box 37 Encapsulation film

[0039] 31 Placement slot 38 Sealing film

[0040] 311 Placement hole 40 Connecting pin

[0041] 32 Liquid storage area 41 Column body

[0042] 321 Sample slot 42 Limiting block

[0043] 322 Liquid storage tank; 43 Positioning plate

[0044] 323 Pretreatment tank 44 Deformation tank

[0045] 323a Ultrasonic bath 50 pipette tip

[0046] 323b Pyrolysis Tank 60 Sealing Cap Detailed Implementation

[0047] The specific embodiments of this utility model will be described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are for illustration and explanation only and are not intended to limit the scope of this utility model.

[0048] The sample testing kit of this utility model is described below with reference to the accompanying drawings.

[0049] like Figures 1 to 3As shown, this utility model provides a sample detection box, which includes a detection tube 10, a cover 20, and a base box 30. The cover 20 has a pipetting hole 21. The base box 30 is integrally formed and rotatably disposed within the cover 20. A placement groove 31 is formed on the side of the base box 30 facing the pipetting hole 21, forming a liquid storage area 32. Multiple placement holes 311 for placing the detection tube 10 are formed on the bottom wall of the placement groove 31. The liquid storage area 32 and the multiple placement holes 311 are arranged circumferentially and can be rotated sequentially to communicate with the pipetting hole 21. It should be noted that the sample detection box of this utility model embodiment can be used for nucleic acid extraction and detection. By placing the collected nucleic acid sample into the sample detection box and installing the sample detection box into a sample detection device, sample pretreatment and detection can be performed.

[0050] Specifically, the bottom box 30 has a placement groove 31 and a liquid storage area 32 arranged in a circumferential direction. The liquid storage area 32 is used to store samples and reagents such as eluent, water, and mineral oil for sample pretreatment. The placement groove 31 has multiple placement holes 311, which are spaced apart along the circumference of the bottom box 30. Each placement hole 311 can be used to place a detection tube 10, so that the same detection reagent or different detection reagents can be stored in the multiple detection tubes 10 respectively. In addition, the cover 20 is provided on the bottom box 30 and can rotate relative to the bottom box 30. The cover 20 has a pipetting hole 21, so that the cover 20 can drive the pipetting hole 21 to rotate to communicate with the liquid storage area 32 or any of the placement holes 311.

[0051] During sample testing, the cover 20 rotates relative to the base box 30 to align the pipetting orifice 21 with the storage area 32, allowing pretreatment of the sample in the storage area 32 through the pipetting orifice 21. After sample pretreatment, the cover 20 rotates relative to the base box 30 to align the pipetting orifice 21 with multiple placement holes 311, allowing the pretreated sample to be injected sequentially into multiple detection tubes 10. Each detection tube 10 can contain the same or different detection reagents. The sample detection kit is then inserted into the sample detection device for testing. This allows for multiple tests on the same indicator, significantly improving testing efficiency, or simultaneous testing of multiple indicators, enabling the detection of multiple pathogens. Furthermore, the base box 30 integrates a liquid storage area 32 and multiple placement holes 311, resulting in a high degree of structural integration and portability. The base box 30 is also a one-piece molded component, offering advantages such as ease of manufacturing, reduced assembly steps, and less assembly error. The cover 20 protects the base box 30 from the introduction of impurities, making it suitable for operation in outdoor environments. The sample testing kit has a wide range of applications and facilitates the immediate detection of samples.

[0052] In this embodiment of the invention, the bottom box 30 includes a panel 33 and a surrounding plate 34. The surrounding plate 34 surrounds the periphery of the panel 33. A placement groove 31 is formed on the side of the panel 33 facing the pipetting hole 21, and a liquid storage area 32 is formed therein. Figure 2 and Figure 3 As shown, panel 33 is located inside cover 20, and surrounding plate 34 extends into cover 20 and rotates with cover 20. Panel 33 has a liquid storage area 32 and a placement groove 31. The liquid storage area 32 and placement groove 31 are arranged in a circumferential direction. The liquid storage area 32 is used for sample pretreatment. The bottom wall of placement groove 31 has multiple placement holes 311. Each placement hole 311 is used to place a detection tube 10, so that the upper end of the detection tube 10 is flush with or lower than the opening of placement groove 31. Placement groove 31 serves to accommodate multiple detection tubes 10 and protect the detection tubes 10, thereby improving the structural stability and portability of the sample detection box.

[0053] Furthermore, the base box 30 also includes multiple limiting sleeves 35, which are disposed on the side of the panel 33 facing away from the pipette hole 21, and each of the multiple limiting sleeves 35 is respectively connected to a multiple placement hole 311. Figures 2 to 4 As shown, a limiting sleeve 35 is provided on the lower side of the panel 33 corresponding to the position of each placement hole 311. The detection tube 10 passes through the placement hole 311 and is inserted into the limiting sleeve 35. The limiting sleeve 35 is used to restrict the movement of the detection tube 10 to stably support the detection tube 10, effectively preventing the detection tube 10 from shaking, and further improving the structural stability and portability of the sample detection box. In addition, the lower end of the detection tube 10 extends out of the limiting sleeve 35. The sample detection device has a fluorescence detection device. The optical fiber of the fluorescence detection device can be connected to the detection tube 10 from the lower end of the limiting sleeve 35 to realize the detection of the sample in the detection tube 10. The structure is reasonably designed and can detect multiple detection tubes 10 at the same time, which improves the detection efficiency.

[0054] In this embodiment of the invention, the bottom box 30 further includes a plurality of reinforcing plates 36, which are spaced apart on the side of the panel 33 facing away from the pipetting hole 21, and the limiting sleeve 35 is located between the surrounding plate 34 and the plurality of reinforcing plates 36. Figures 2 to 4 As shown, a plurality of reinforcing plates 36 are arranged at intervals along the circumference of the panel 33 on the lower side of the panel 33. A plurality of limiting sleeves 35 are arranged around the outside of the plurality of reinforcing plates 36, and a surrounding plate 34 is arranged around the outside of the plurality of limiting sleeves 35. The reinforcing plates 36 strengthen the structural strength of the panel 33 and improve the structural stability of the sample detection box, making it suitable for carrying outdoors for immediate sample detection.

[0055] In this embodiment of the invention, a connection hole 332 is provided on the panel 33, and the placement groove 31 and the liquid storage area 32 surround the outer periphery of the connection hole 332. The sample detection box also includes a connecting pin 40, one end of which rotatably passes through the connection hole 332 and is connected to the panel 33, and the other end of which is connected to the cover 20. Figures 1 to 6 As shown, a connection hole 332 is provided in the center of the panel 33. The connecting pin 40 passes through the connection hole 332 and the cover 20. The two ends of the connecting pin 40 are respectively connected to the lower side of the panel 33 and the upper side of the cover 20 to limit the cover 20 to the bottom box 30 and prevent the cover 20 from detaching from the bottom box 30. The structure is stable and reliable. In addition, the connecting pin 40 can rotate relative to the connection hole 332 so that the cover 20 can drive the connecting pin 40 to rotate relative to the bottom box 30. This allows the pipetting hole 21 to communicate with the liquid storage area 32 and multiple placement holes 311 in sequence, which facilitates liquid transfer through the pipetting hole 21 and effectively prevents impurities from being introduced into the liquid storage area 32 and the placement holes 311, thereby improving the detection accuracy.

[0056] Furthermore, a positioning groove 22 is provided on the side of the cover 20 facing away from the bottom box 30. The connecting pin 40 includes a pin body 41, a limiting block 42, and a positioning piece 43. The limiting block 42 and the positioning piece 43 are respectively provided at both ends of the pin body 41. The pin body 41 passes through the bottom wall of the positioning groove 22 and the connecting hole 332. The limiting block 42 abuts against the side of the panel 33 facing away from the pipetting hole 21. The positioning piece 43 is located in the positioning groove 22. Figures 1 to 6 As shown, a positioning groove 22 is provided on the upper side of the cover 20. The column body 41 passes through the connecting hole 332 and the cover 20. The positioning piece 43 is connected to the upper end of the column body 41 and installed in the positioning groove 22. The positioning groove 22 is used to restrict the rotation of the positioning piece 43 so that the cover 20 can be connected to the column body 41 through the positioning piece 43 and drive the column body 41 to rotate relative to the bottom box 30. The limiting block 42 is connected to the lower end of the column body 41 and abuts against the lower side of the panel 33 to limit the cover 20 to the bottom box 30 and prevent the cover 20 from falling off the bottom box 30. The structure is stable and reliable.

[0057] In the embodiments of this utility model, such as Figure 4 and Figure 6 As shown, a connecting sleeve 333 communicating with the connecting hole 332 is provided on the lower side of the panel 33 at the position corresponding to the connecting hole 332. The column body 41 passes through the connecting hole 332 and the connecting sleeve 333 in sequence, so that the limiting block 42 extends to the lower end of the connecting sleeve 333 and abuts against the lower end of the connecting sleeve 333 to limit the cover 20 on the bottom box 30, preventing the cover 20 from detaching from the bottom box 30. The structure is stable and reliable. The connecting sleeve 333 is used to limit the movement of the column body 41 and allow the column body 41 to rotate within the connecting sleeve 333, which improves the structural stability and smoothness of rotation.

[0058] Furthermore, such as Figure 4 and Figure 6 As shown, the lower end of the column body 41 is provided with multiple spaced deformation grooves 44. When installing the connecting column 40, the limiting block 42 needs to pass through the connecting sleeve 333. The inner wall of the connecting sleeve 333 squeezes the limiting block 42, causing the limiting block 42 to move into the deformation groove 44, which facilitates the contraction of the limiting block 42 and its passage through the connecting sleeve 333. The deformation groove 44 provides deformation space for the movement of the limiting block 42. The structure is reasonably designed and improves the ease of assembly.

[0059] In the embodiments of this utility model, such as Figure 1 , Figure 3 and Figure 5 As shown, a positioning protrusion 25 is formed on the cover 20, and a groove 341 is provided on the bottom box 30 at the position corresponding to the positioning protrusion 25 for the positioning protrusion 25 to extend into, so as to position and install the cover 20 onto the bottom box 30, preventing the cover 20 from rotating relative to the bottom box 30 before using the sample detection box, thus ensuring the initial performance stability of the sample detection box. Furthermore, when performing sample detection, the cover 20 rotates relative to the bottom box 30 to tear off the positioning protrusion 25, so as to facilitate pipetting through the pipetting hole 21.

[0060] In the embodiments of this utility model, such as Figures 2 to 4 As shown, the upper side of the panel 33 is provided with a sealing film 37, which is used to seal the liquid storage area 32. The lower side of the panel 33 is provided with a sealing film 38, which is used to prevent impurities or contaminants from entering the housing 20 through the connecting sleeve 333 and the connecting hole 332, causing contamination or interference with the detection. Each detection tube 10 is provided with a sealing film 11, which is used to seal the detection tube 10 to prevent contamination of the detection reagent. The sealing film 37, sealing film 38 and sealing film 11 all play a role in improving the overall sealing performance.

[0061] In this embodiment of the invention, a sample tank 321, a storage tank 322, and a pretreatment tank 323 are provided in the liquid storage area 32. The sample tank 321, the storage tank 322, and the pretreatment tank 323 are spaced apart and can be rotated sequentially to communicate with the pipetting hole 21. Figure 2 and Figure 3As shown, the storage area 32 is provided with independent sample tank 321, storage tank 322, and pretreatment tank 323. The number of sample tank 321, storage tank 322, and pretreatment tank 323 can be one or more. The sample tank 321 is used to hold the sample, the storage tank 322 is used to hold eluent, water, mineral oil, and other reagents for sample pretreatment, and the pretreatment tank 323 can be used to pretreatment the sample. The sample detection box is inserted into the sample detection device so that the pretreatment device of the sample detection device can perform ultrasonic treatment or magnetic attraction treatment on the sample transferred to the pretreatment tank 323. Understandably, the pretreatment tank 323 can contain magnetic beads for magnetic attraction treatment of the sample. The sample detection box has a high degree of integration, is easy to carry, and is conducive to realizing the immediate detection of the sample.

[0062] Furthermore, a storage slot 324 is provided within the liquid storage area 32, and a pick-and-place hole 23 is provided on the cover 20 at the position corresponding to the storage slot 324. The sample detection box also includes a pipette tip 50, which passes through the pick-and-place hole 23 and extends into the storage slot 324. Figure 2 and Figure 3 As shown, the pipette tip 50 is used for pipetting. The pick-and-place hole 23 allows the pipette tip 50 to pass through the cover 20 and extend into the receiving groove 324, so that the receiving groove 324 can accommodate the pipette tip 50. When the sample detection box is placed into the sample detection equipment, the pipetting device of the sample detection equipment picks up the pipette tip 50 from the pick-and-place hole 23 and drives the pipette tip 50 through the pipetting hole 21 for pipetting. The structure has a high degree of integration and prevents impurities from entering the cover 20 and causing detection contamination, which is conducive to realizing the immediate detection of samples.

[0063] In this embodiment of the invention, the housing 20 is further provided with positioning holes 24 spaced apart from the pipetting holes 21. For example... Figure 2 and Figure 5 As shown, the positioning hole 24 is used for the jaws of the pipetting device to extend into, so that the pipetting device can rotate the cover 20 relative to the bottom box 30 by rotating the jaws, thereby causing the pipetting hole 21 to rotate to communicate with the liquid storage area 32 or any of the placement holes 311, so that the pipetting tip 50 can extend into the cover 20 from the pipetting hole 21 to perform pipetting.

[0064] In this embodiment of the invention, the sample detection box further includes a sealing cap 60 covering the pipette hole 21; as shown Figure 1 and Figure 2 As shown, the sealing cap 60 is used to seal the pipette hole 21, preventing impurities or contaminants from entering the housing 20 through the pipette hole 21 before the sample detection kit is used, thus ensuring the initial performance stability of the sample detection kit.

[0065] In this embodiment of the present invention, during the assembly of the sample detection box, the sealing cap 60 is first installed at the pipetting hole 21, the connecting pin 40 is passed through the cover 20, and the pipette tip 50 is passed through the pick-and-place hole 23. Then, the sealing film 38 is heat-sealed onto the lower side of the panel 33. Eluents, water, mineral oil, and other reagents used for sample pretreatment are injected into the corresponding storage tanks 322, and magnetic beads are placed into the corresponding pretreatment tanks 323. Finally, the encapsulation film 37 is heat-sealed onto the upper side of the panel 33 to seal the storage area 32. The test reagent is injected into each of the multiple test tubes 10 or a lyophilized bulb 12 for testing is placed inside. Each test tube 10 is sealed with a sealing film 11, and the sealed test tubes 10 are placed into the multiple placement holes 311 one by one. Finally, the cover 20 is installed on the base box 30 so that the connecting pin 40 passes through the connecting hole 332 and the pipette tip 50 extends into the storage slot 324. The assembly of the sample test box is then completed. The sample test box has a high degree of structural integration and is easy to carry, which is conducive to the immediate detection of samples.

[0066] In the embodiments of this utility model, such as Figure 2 and Figure 3 As shown, the storage area 32 contains a sample tank 321, five storage tanks 322, two pretreatment tanks 323, and a protease tank 325. The sample tank 321 is used to hold samples. The five storage tanks 322 are a washing tank, a first elution tank, a second elution tank, a third elution tank, and a mineral oil tank, respectively. The washing tank contains water, the first, second, and third elution tanks contain elution solution, and the mineral oil tank contains mineral oil. The two pretreatment tanks 323 are respectively... The ultrasonic tank 323a and the lysis tank 323b contain magnetic beads for magnetically attracting samples. The protease tank 325 contains protease. In addition, multiple detection tubes 10 contain lyophilized bulbs 12 for detecting samples. The lyophilized bulbs 12 are made by freeze-drying the detection reagents. This allows the lyophilized bulbs 12 in multiple detection tubes 10 to be used to detect the same indicator or to detect different indicators separately, which greatly improves the detection efficiency and realizes the simultaneous detection of multiple pathogens.

[0067] During sample testing, the operator opens the sealing cap 60 and injects the sample into the sample tank 321. After sample addition, the sample testing kit is placed into the sample testing equipment. The heating device of the sample testing equipment preheats the lysis tank 323b. The pipette of the sample testing equipment descends so that the jaws of the pipette extend into the positioning hole 24 and the pipette pump of the pipette picks up the pipette tip. The pipette drives the cover 20 to rotate through the jaws so that the pipetting hole 21 communicates with the sample tank 321. The pipette tip 50 draws the sample from the sample tank 321 through the pipetting hole 21 and injects the sample into the ultrasonic tank 323a. The pretreatment device of the sample testing equipment performs ultrasonic lysis on the sample in the ultrasonic tank 323a. After ultrasonic lysis, the pipette tip 50 transfers the sample to the lysis tank 323b, and the pipette tip 50 draws the protease from the protease tank 325 and transfers it to the lysis tank 323b. The heating device preheats the lysis tank 323b. The sample in tank 323b is heated and lysed. The pretreatment device uses a magnet to attract magnetic beads. After attraction, the pipette tip 50 transfers the waste liquid to sample tank 321. The pipette tip 50 also draws eluent from the first elution tank and transfers it to lysis tank 323b, and from the second elution tank and transfers it to lysis tank 323b. The pretreatment device removes the magnet, and the pipette device drives the pipette tip 50 to repeatedly blow and mix the sample in lysis tank 323b. Then, the pretreatment device uses a magnet to attract magnetic beads again. After attraction, the pipette tip 50 transfers the waste liquid to the first and second elution tanks respectively. After the waste liquid is transferred, the pipette tip 50 is inserted into the washing tank for washing. After washing, the pipette tip 50 draws eluent from the third elution tank and transfers it to lysis tank 323b. The sample is then eluted again by magnetic attraction and mixing with the magnetic beads using a magnet, thus completing the sample pretreatment.

[0068] Furthermore, during the magnetic suction process, the pipette tip 50 is inserted into the second elution tank for secondary cleaning. After cleaning, the pipetting device drives the pipette tip 50 to puncture the sealing film 11 of multiple detection tubes 10 in sequence. The pipette tip 50 draws an appropriate amount of pretreated sample from the lysis tank 323b and injects it into multiple detection tubes 10. The pretreated sample is then thawed and mixed with the lyophilized bulbs 12. After the sample has been injected into multiple detection tubes 10, the pipette tip 50 draws mineral oil from the mineral oil tank and injects it into multiple detection tubes 10 to seal the sample. The pipette tip 50 is then reset, and the fluorescence detection device detects multiple detection tubes 10. This allows for multiple simultaneous detections of one detection index or multiple detection indexes, significantly improving detection efficiency.

[0069] The sample detection kit of this utility model is compatible with both liquid PCR and lyophilized systems. It is small in size, highly integrated, and easy to carry. The pretreatment and detection processes are completely sealed, eliminating the need for professional operators and standard laboratory environments. It is suitable for on-the-spot testing in outdoor environments and has a wide range of applications. Furthermore, during the pretreatment and detection processes, only manual sample injection by the operator is required. All other pretreatment and detection operations do not require manual intervention, reducing reliance on professional operators and standard laboratory environments and improving detection efficiency and the accuracy of test results.

[0070] In addition, this utility model also provides a sample detection device, which includes a sample detection box according to the above description. The specific structure of the sample detection box is as described in the above embodiments. Since the sample detection device adopts all the technical solutions of the above embodiments, it has at least all the beneficial effects brought about by the technical solutions of the above embodiments, which will not be described in detail here.

[0071] In the description of this utility model, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this utility model, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0072] In this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, an electrical connection, or a connection that allows communication between them; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0073] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0074] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention.

Claims

1. A sample detection kit, characterized in that, The sample detection kit includes: Detection tube (10); A cover (20) is provided with a pipetting hole (21); The bottom box (30) is integrally formed and can be rotatably disposed inside the cover (20). The bottom box (30) has a placement groove (31) and a liquid storage area (32). The bottom wall of the placement groove (31) has multiple placement holes (311) for placing the detection tube (10). The liquid storage area (32) and the multiple placement holes (311) are arranged in a circumferential interval and can be rotated sequentially to communicate with the pipetting hole (21).

2. The sample detection kit according to claim 1, characterized in that, The bottom box (30) includes a panel (33) and a surrounding panel (34). The surrounding panel (34) surrounds the periphery of the panel (33). The panel (33) has the placement groove (31) and the liquid storage area (32) formed on the side facing the pipetting hole (21).

3. The sample detection kit according to claim 2, characterized in that, The bottom box (30) also includes a plurality of limiting sleeves (35), which are disposed on the side of the panel (33) facing away from the pipetting hole (21), and the plurality of limiting sleeves (35) are respectively connected to the plurality of placement holes (311).

4. The sample detection kit according to claim 3, characterized in that, The bottom box (30) also includes a plurality of reinforcing plates (36), which are spaced apart on the side of the panel (33) facing away from the pipetting hole (21), and the limiting sleeve (35) is located between the surrounding plate (34) and the plurality of reinforcing plates (36).

5. The sample detection kit according to claim 2, characterized in that, The panel (33) has a connection hole (332), the placement slot (31) and the liquid storage area (32) are arranged around the outer periphery of the connection hole (332), the sample detection box also includes a connecting pin (40), one end of the connecting pin (40) is rotatably passed through the connection hole (332) and connected to the panel (33), and the other end of the connecting pin (40) is connected to the cover (20).

6. The sample detection kit according to claim 5, characterized in that, The cover (20) has a positioning groove (22) on the side facing away from the bottom box (30). The connecting pin (40) includes a pin body (41), a limiting block (42) and a positioning piece (43). The limiting block (42) and the positioning piece (43) are respectively located at both ends of the pin body (41). The pin body (41) passes through the bottom wall of the positioning groove (22) and the connecting hole (332). The limiting block (42) abuts against the side of the panel (33) facing away from the pipetting hole (21). The positioning piece (43) is located in the positioning groove (22).

7. The sample detection kit according to any one of claims 1 to 6, characterized in that, The liquid storage area (32) is provided with a sample tank (321), a liquid storage tank (322) and a pretreatment tank (323). The sample tank (321), the liquid storage tank (322) and the pretreatment tank (323) are arranged at intervals and can be rotated in sequence to communicate with the pipetting hole (21).

8. The sample detection kit according to claim 7, characterized in that, The liquid storage area (32) is also provided with a storage slot (324), and the cover (20) is provided with a pick-up and put-out hole (23) corresponding to the position of the storage slot (324). The sample detection box also includes a pipette tip (50), which passes through the pick-up and put-out hole (23) and extends into the storage slot (324).

9. The sample detection kit according to any one of claims 1 to 6, characterized in that, The sample detection kit also includes a sealing cap (60) covering the pipette hole (21); And / or, the housing (20) is also provided with positioning holes (24) spaced apart from the pipetting hole (21).

10. A sample testing device, characterized in that, The sample testing device includes a sample testing box according to any one of claims 1 to 9.

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