Micro-fluidic chip liquid temperature measurement system

Abstract

The utility model discloses a micro-fluidic chip liquid temperature measurement system, which comprises a temperature measurement element and an adapter plate which are arranged on a driving electrode of a set temperature measurement area of a lower substrate of a micro-fluidic chip, and the end part of a signal line of the temperature measurement element extends to the lower plate surface of the lower substrate and is connected with a corresponding welding spot of a first contact bonding pad arranged on the lower plate surface of the lower substrate; a second contact bonding pad and a flat cable seat are arranged on the adapter plate, a welding spot on the second contact bonding pad is matched with a welding spot on the first contact bonding pad, and the welding spot on the second contact bonding pad is respectively connected with a wiring terminal corresponding to the flat cable seat through a signal line; the welding spots on the first contact bonding pad are respectively connected with the corresponding welding spots on the second contact bonding pad through conductive columns; the flat cable seat is used for connecting an external temperature measurement system. According to the utility model, the temperature of the reaction liquid at a specified point in the micro-fluidic chip is directly tested, so that the temperature detection precision, efficiency and reliability of the liquid drops in the reaction bin of the micro-fluidic chip are greatly improved.

Description

Technical Field

[0001] This utility model relates to microfluidic chips, and more particularly to a microfluidic chip liquid temperature measurement system. Background Technology

[0002] Digital microfluidics generally refers to the control of droplet movement using electrowetting (EWOD) technology. Based on the numerous advantages of digital microfluidic chips, much research has emerged on microfluidic chip structure design and precise droplet manipulation. However, besides the volume and precision of droplet movement, many applications require specific temperatures, such as PCR (polymerase chain reaction). Therefore, the actual temperature detection of the reaction liquid inside the microfluidic chip directly affects the PCR reaction results.

[0003] Chinese patent CN113751089A, "A Digital Microfluidic Chip Integrated with a Heating Module," achieves integrated, feedback-based temperature control of the microfluidic chip by integrating resistance wire circuits for heating and temperature measurement on the chip surface. Chinese patent CN215757374U, "A Digital PCR Microfluidic Chip Temperature Measurement Module," creates grooves on the microfluidic chip, places a temperature sensor within these grooves, and positions the sensor at the temperature to be measured. However, the technical solutions disclosed in these patents do not achieve direct detection of the droplet temperature within the microfluidic chip's flow channels. The temperature detection results are significantly affected by ambient temperature and other external factors, causing the PCR results to fall short of expectations. Summary of the Invention

[0004] The purpose of this invention is to provide a microfluidic chip liquid temperature measurement system that enables direct monitoring of real-time PCR temperature with high measurement accuracy.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] The microfluidic chip liquid temperature measurement system of this utility model includes a temperature measuring element and an adapter board disposed on the driving electrode of the temperature measuring area set on the lower substrate of the microfluidic chip. The signal line end of the temperature measuring element extends to the lower surface of the lower substrate and is connected to the corresponding solder point of the first contact pad disposed on the lower surface of the lower substrate. The adapter board is provided with a second contact pad and a ribbon cable connector. The solder points on the second contact pad match the solder points on the first contact pad. The solder points on the second contact pad are respectively connected to the corresponding terminals of the ribbon cable connector through signal lines. The solder points on the first contact pad are respectively connected to the corresponding solder points on the second contact pad through conductive posts. The ribbon cable connector is used to connect to an external temperature measurement system.

[0007] Alternatively, the conductive post is an elastic conductive post, which consists of a hollow column, springs and ball bearings disposed at both ends of the hollow column.

[0008] Alternatively, the temperature sensing element may be a thermistor, thermocouple, or PT1000 temperature sensor.

[0009] This invention places the temperature sensing element on the driving electrode at a preset temperature sensing point on the lower substrate of the microfluidic chip, directly testing the temperature of the reaction liquid at a specified point inside the microfluidic chip, and outputting the temperature signal detected by the temperature sensing element to an external temperature measurement system through an adapter board, which greatly improves the accuracy, efficiency and reliability of droplet temperature detection in the reaction chamber of the microfluidic chip. Attached Figure Description

[0010] Figure 1 This is a schematic diagram of the temperature sensing element described in this utility model being disposed on the driving electrode of a microfluidic chip.

[0011] Figure 2 This is a schematic diagram of the first contact pad located on the lower surface of the substrate under the microfluidic chip.

[0012] Figure 3 This is a schematic diagram of the adapter plate described in this utility model.

[0013] Figure 4 This is a schematic diagram showing the first and second contact pads connected by the elastic conductive pillar. Detailed Implementation

[0014] The embodiments of this utility model will be described in detail below with reference to the accompanying drawings. These embodiments are implemented based on the technical solution of this utility model and provide detailed implementation methods and specific operation processes. However, the protection scope of this utility model is not limited to the following embodiments.

[0015] It should be noted that in the description of this utility model, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations.

[0016] In the description of this utility model, unless otherwise explicitly specified and limited, the terms "connected" and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0017] like Figure 1-3As shown, the microfluidic chip liquid temperature measurement system of this utility model includes a temperature measuring element 3 and an adapter plate 4 disposed on a driving electrode 2 at a preset temperature measuring point on the lower substrate 1 of the microfluidic chip; the number of temperature measuring zones is determined according to the needs of the PCR experiment.

[0018] like Figure 1 As shown, the microfluidic chip consists of an upper substrate 5 and a lower substrate 1 spaced apart, forming a microchannel 7 between the upper and lower substrates for the movement of droplets 6. An array of driving electrodes 2 is arranged along the designated microchannel 7 to drive the droplets 6 to move along the microchannel. A temperature sensing element 3 is placed on the driving electrode 2 at a designated temperature sensing area, thereby achieving direct real-time detection of the temperature of the droplets 6 within the microchannel. This significantly improves the accuracy and reliability of temperature detection of the droplets 6 within the reaction chamber of the microfluidic chip, providing accurate data for PCR detection results.

[0019] like Figure 2 , 3 As shown, the end of the signal line 3.1 of the temperature sensing element 3 extends to the lower surface of the lower substrate 1 and is connected to the corresponding solder point of the first contact pad 8 provided on the lower surface of the lower substrate 1; the adapter plate 4 is provided with a second contact pad 4.1 and a ribbon cable holder 4.2, the solder point on the second contact pad 4.1 matches the solder point on the first contact pad 8, and the solder point on the second contact pad 4.1 is connected to the corresponding terminal of the ribbon cable holder 4.2 through the signal line 4.3.

[0020] like Figure 4 As shown, the solder joints on the first contact pad 8 are connected to the corresponding solder joints on the second contact pad 4.1 via conductive posts 9; the ribbon cable connector 4.2 is used to connect to an external temperature measurement system and send the temperature signal detected by the temperature measuring element 3 to the external temperature measurement system.

[0021] Advantageously or exemplaryly, the conductive post 9 is an elastic conductive post, which consists of a hollow column and springs and balls disposed at both ends of the hollow column; the elastic conductive post facilitates the connection between the first and second contact pads 8 and 4.1 solder joints.

[0022] Advantageously or exemplary, the temperature sensing element 3 may be selected from a thermistor, thermocouple or PT1000 temperature sensor.

[0023] The external temperature measurement system is connected via ribbon cable connector 4.2. The signal line 4.3 on the adapter board 4 uses a four-wire routing system to improve temperature measurement accuracy. The temperature measurement system uses a high-frequency MCU microcontroller, which communicates with the analog-to-digital converter (ADC) via SPI. The ADC performs differential acquisition on the temperature measuring device 3, and by reading the relevant AD values, the temperature of each channel is calculated by software.

[0024] Finally, it should be emphasized that the above-described technical solutions are merely preferred embodiments of this utility model and are not intended to limit this utility model. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still make modifications to the technical solutions described in the foregoing embodiments without creative effort, or make equivalent substitutions for some of the technical features. Therefore, any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A microfluidic chip liquid temperature measurement system, characterized in that: The device includes a temperature sensing element and an adapter board disposed on the driving electrode of the set temperature zone on the lower substrate of the microfluidic chip. The signal line end of the temperature sensing element extends to the lower surface of the lower substrate and is connected to the corresponding solder point of the first contact pad disposed on the lower surface of the lower substrate. The adapter board is provided with a second contact pad and a ribbon cable connector. The solder points on the second contact pad match the solder points on the first contact pad. The solder points on the second contact pad are respectively connected to the corresponding terminals of the ribbon cable connector through signal lines. The solder points on the first contact pad are respectively connected to the corresponding solder points on the second contact pad through conductive posts. The ribbon cable connector is used to connect to an external temperature sensing system.

2. The microfluidic chip liquid temperature measurement system according to claim 1, characterized in that: The conductive post is an elastic conductive post, which consists of a hollow column, springs and ball bearings disposed at both ends of the hollow column.

3. The microfluidic chip liquid temperature measurement system according to claim 1 or 2, characterized in that: The temperature sensing element is a thermistor, thermocouple, or PT1000 temperature sensor.

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