Biological tissue collecting device and system

Abstract

The utility model relates to a biological tissue collecting device and system. The biological tissue collecting device comprises an inner tube unit, an adsorption unit and an outer tube unit, the device has the advantages that the inner tube unit, the outer tube unit and the container unit are detachably arranged, so that the inner tube unit is conveniently separated from the container unit when a tissue sample is adsorbed subsequently, the inner tube unit and the container unit are independently used, and the tissue sample can be adsorbed on the adsorption unit through the cooperative use of the adsorption unit and the inner tube unit; the operation of repeatedly using a medicine spoon to dig or pour is replaced, the tissue sample is prevented from being poured and scattered from the medicine spoon, and the reliability of transferring the tissue sample is guaranteed; the inner tube unit is sealed by the sealing unit, so that a tissue sample in the inner tube unit is prevented from being moved out, and the sealing performance of the inner tube unit is guaranteed; and the switching unit can be communicated with pipelines of negative pressure devices with different sizes, so that the assembly applicability is improved.

Description

Technical Field

[0001] This utility model relates to the technical field of biological tissue instruments, and in particular to a biological tissue collection device and system. Background Technology

[0002] Centrifuge tubes (EP tubes) are frequently used in medical and biological experiments. One of their purposes is to collect tissue samples and extract RNA or proteins from them. For this purpose, centrifuge tubes are usually filled with lysis buffer. Researchers grind frozen biological tissue samples in liquid nitrogen, then place the tissue powder into the centrifuge tube and immerse it in the lysis buffer to lyse the RNA or proteins in the tissue. After further centrifugation and other procedures, researchers can extract the desired components, such as RNA and proteins, from the biological tissue sample.

[0003] When extracting RNA and protein from tissue samples, liquid nitrogen is generally used to grind the tissue into tissue fragments, which allows for full contact with the lysis buffer and increases lysis efficiency.

[0004] During liquid nitrogen grinding, the tissue sample and liquid nitrogen are poured into a mortar and pestle and ground to break the tissue sample into small pieces. Care should be taken not to use excessive force to prevent tissue sample pieces from splashing out of the mortar.

[0005] The process of transferring tissue samples ground with liquid nitrogen into centrifuge tubes containing lysis buffer using a spatula presents several challenges. This involves using a spatula to scoop out the tissue sample and pour it into the centrifuge tube. This process is time-consuming and labor-intensive, carries the risk of frostbite to the experimenter's hands, and is prone to spillage during pouring, resulting in waste and impacting the experiment.

[0006] Currently, no effective solutions have been proposed for the problems of time-consuming and laborious operation processes, the risk of experimental personnel's hands being frostbitten by liquid nitrogen, and the tendency to spill when pouring into centrifuge tubes. Utility Model Content

[0007] The purpose of this invention is to address the shortcomings of existing technologies by providing a biological tissue collection device and system, thereby solving the problems of time-consuming and laborious operation, the risk of experimental personnel's hands being frostbitten by liquid nitrogen, and the tendency to spill when pouring into centrifuge tubes.

[0008] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0009] Firstly, a biological tissue collection device is provided, comprising:

[0010] An inner tube unit is removably disposed inside the container device and detachably connected to a negative pressure device for adsorbing a mixed liquid containing biological tissue under the action of the negative pressure device.

[0011] An adsorption unit is disposed at the bottom of the inner tube unit and is used to filter the mixed liquid containing biological tissue under the action of a negative pressure device to retain the biological tissue, and to immerse the biological tissue in the lysis solution of the container device when the inner tube unit is disposed inside the container device.

[0012] An outer tube unit is disposed on top of the inner tube unit and is detachably connected to the container device.

[0013] In some embodiments, the inner tube unit includes:

[0014] An inner tube element, the bottom end of which is connected to the adsorption unit, and the top end of which is connected to the outer tube unit, is removably disposed inside the container device and detachably connected to a negative pressure device, for adsorbing mixed liquid containing biological tissue under the action of the negative pressure device.

[0015] In some embodiments, the outer tube unit includes:

[0016] An outer tube element is disposed on the outer edge surface of the top end of the inner tube unit;

[0017] A first connecting element is disposed on the inner edge surface of the top end of the outer tube element and is connected to the outer tube element and the inner tube unit respectively.

[0018] In some embodiments, the inner tube unit further includes:

[0019] The second connecting element is disposed at the top end of the inner tube element and is detachably connected to the container device.

[0020] In some embodiments, the outer tube unit further includes:

[0021] A third connecting element is disposed on the inner edge surface of the outer tube element and located below the first connecting element, and is detachably connected to the container device.

[0022] In some of these embodiments, it also includes:

[0023] A sealing unit, which is removably disposed on top of the inner tube unit, is used to seal the inner tube unit.

[0024] In some embodiments, the outer tube unit further includes:

[0025] A fourth connecting element is disposed on the outer edge surface of the outer tube unit and is detachably connected to the sealing unit.

[0026] In some embodiments, the sealing unit includes:

[0027] A sealing element, which is removably disposed on the top of the inner tube unit for sealing the inner tube unit;

[0028] The fifth connecting element is disposed at the bottom end or inner edge of the sealing element and is detachably connected to the inner tube unit or the outer tube unit.

[0029] In some of these embodiments, it also includes:

[0030] The adapter unit has a first end that is detachably connected to the inner tube unit or the outer tube unit, and a second end that is detachably connected to the negative pressure device.

[0031] In some embodiments, the inner tube unit further includes:

[0032] A sixth connecting element is disposed on the outer edge of the bottom end of the inner tube unit and is detachably connected to the first end of the adapter unit.

[0033] In some embodiments, the outer tube unit further includes:

[0034] A fourth connecting element is disposed on the outer edge surface of the outer tube unit and is detachably connected to the first end of the adapter unit.

[0035] In some embodiments, the switching unit includes:

[0036] Adapter components;

[0037] A seventh connecting element is disposed at the first end of the adapter element and is detachably connected to the inner tube unit or the outer tube unit.

[0038] The eighth connecting element is disposed at the second end of the adapter element and is detachably connected to the negative pressure device.

[0039] In some of these embodiments, it also includes:

[0040] A container unit, wherein the inner tube unit is removably disposed inside the container unit and is detachably connected to the inner tube unit or the outer tube unit, is used to store pyrolysis fluid.

[0041] In some embodiments, the container unit includes:

[0042] A container element, wherein the inner tube unit is removably disposed inside the container element and is detachably connected to the inner tube unit or the outer tube unit, for storing pyrolysis fluid.

[0043] In some embodiments, the container unit further includes:

[0044] A ninth connecting element is disposed at the top of the container element and is detachably connected to the inner tube unit or the outer tube unit.

[0045] In some embodiments, the container unit further includes:

[0046] A marking element is disposed on the outer edge surface of the container element for indicating the volume of lysate within the container element.

[0047] In some of these embodiments, it also includes:

[0048] The conveying unit has a first end that is detachably connected to the inner tube unit and a second end that is detachably connected to the negative pressure device.

[0049] Secondly, a biological tissue collection system is provided, comprising:

[0050] The biological tissue collection device as described in the first aspect;

[0051] A negative pressure device is provided, which is detachably connected to the inner tube unit of the biological tissue collection device.

[0052] In some of these embodiments, it also includes:

[0053] A grinding device, wherein the top end of the inner tube unit of the biological tissue collection device is removably disposed inside the grinding device, for grinding biological tissue in a liquid nitrogen environment.

[0054] In some of these embodiments, it also includes:

[0055] A centrifuge apparatus, wherein a container device or the container unit of the biological tissue collection device is removably disposed inside the centrifuge apparatus for centrifuging and separating biological tissue and lysate.

[0056] The present invention adopts the above technical solution and has the following technical effects compared with the prior art:

[0057] This invention discloses a biological tissue collection device and system. The inner tube unit, outer tube unit, and container unit are detachably configured, allowing for separation of the inner tube unit and container unit during tissue sample adsorption. This enables independent use of each unit. The adsorption unit and inner tube unit work together to adsorb tissue samples onto the adsorption unit, replacing repeated use of a spatula and preventing sample spillage during transfer. A sealing unit further seals the inner tube unit, preventing sample leakage and ensuring its airtightness. An adapter unit allows connection to pipes of different sizes of negative pressure devices, improving assembly versatility. A delivery unit connects the inner tube unit to the negative pressure device via a transfer connector, diversifying the connection options. Attached Figure Description

[0058] Figure 1 This is a three-dimensional structural schematic diagram (I) of a biological tissue collection device according to an embodiment of the present invention;

[0059] Figure 2 This is an exploded view (I) of a biological tissue collection device according to an embodiment of the present invention;

[0060] Figure 3 This is a schematic diagram of the internal structure of the inner tube unit according to an embodiment of the present utility model (I);

[0061] Figure 4 This is a schematic diagram of the internal structure of the outer tube unit according to an embodiment of the present utility model;

[0062] Figure 5 This is a three-dimensional structural schematic diagram (II) of the biological tissue collection device according to an embodiment of the present utility model;

[0063] Figure 6 This is an exploded view (II) of a biological tissue collection device according to an embodiment of the present invention;

[0064] Figure 7 This is a three-dimensional structural schematic diagram (III) of the biological tissue collection device according to an embodiment of the present utility model;

[0065] Figure 8 This is a three-dimensional structural schematic diagram of the outer tube unit according to an embodiment of the present utility model;

[0066] Figure 9 This is a schematic diagram of the internal structure of the sealing unit according to an embodiment of the present utility model;

[0067] Figure 10 This is a schematic diagram (II) of the internal structure of the inner tube unit according to an embodiment of the present utility model;

[0068] Figure 11 This is a three-dimensional structural diagram of the adapter unit according to an embodiment of the present utility model;

[0069] Figure 12 This is a three-dimensional structural schematic diagram of the container unit according to an embodiment of the present utility model;

[0070] Figure 13 This is a schematic diagram of the collection system according to an embodiment of the present utility model.

[0071] The attached figures are labeled as follows: 100, biological tissue collection device;

[0072] 110. Inner tube unit; 111. Inner tube component; 112. Second connecting element; 113. Sixth connecting element;

[0073] 120. Adsorption unit;

[0074] 130. Outer tube unit; 131. Outer tube component; 132. First connecting element; 133. Third connecting element; 134. Fourth connecting element;

[0075] 140. Sealing unit; 141. Sealing element; 142. Fifth connecting element;

[0076] 150. Adapter unit; 151. Adapter element; 152. Seventh connecting element; 153. Eighth connecting element;

[0077] 160. Container unit; 161. Container element; 162. Ninth connecting element; 163. Marking element;

[0078] 170. Conveying unit;

[0079] 200. Negative pressure device; 300. Grinding device; 400. Centrifugal device. Detailed Implementation

[0080] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0081] It should be noted that, unless otherwise specified, the embodiments and features described in these embodiments can be combined with each other.

[0082] The present invention will be further described below with reference to the accompanying drawings and specific embodiments, but this is not intended to limit the present invention.

[0083] Example 1

[0084] This embodiment relates to the biological tissue collection device of this utility model.

[0085] like Figure 1 , Figure 2 As shown, a biological tissue collection device 100 includes an inner tube unit 110, an adsorption unit 120, and an outer tube unit 130. The inner tube unit 110 is removably disposed inside a container device and detachably connected to a negative pressure device, used to adsorb a mixed liquid containing biological tissue under the action of the negative pressure device. The adsorption unit 120 is disposed at the bottom of the inner tube unit 110, used to filter the mixed liquid containing biological tissue under the action of the negative pressure device to retain the biological tissue, and to immerse the biological tissue in the lysis solution of the container device when the inner tube unit 110 is disposed inside the container device. The outer tube unit 130 is disposed at the top of the inner tube unit 110 and detachably connected to the container device.

[0086] In this invention, the container device includes, but is not limited to, test tubes, centrifuge tubes, etc.

[0087] In this invention, the biological tissue collection device 100 is detachably connected to the container device, including but not limited to plug-in and detachable connections.

[0088] like Figure 3 As shown, the inner tube unit 110 includes an inner tube element 111. The bottom end of the inner tube element 111 is connected to the adsorption unit 120, and the top end of the inner tube element 111 is connected to the outer tube unit 130. The inner tube element 111 is removably disposed inside the container device and is detachably connected to the negative pressure device for adsorbing mixed liquid containing biological tissue under the action of the negative pressure device.

[0089] The inner tube element 111 has a hollow structure.

[0090] The inner tube element 111 has a circular cross-section. The longitudinal section of the inner tube element 111 is rectangular.

[0091] The dimensions of the inner tube element 111 are matched with the dimensions of the container assembly. Generally, the radial dimension (e.g., outer diameter) of the outer edge of the inner tube element 111 is not greater than the radial dimension (e.g., inner diameter) of the inner edge of the container assembly, and the axial dimension (e.g., length or height) of the inner tube element 111 is smaller than the axial dimension (e.g., length or height) of the container assembly.

[0092] In some of these embodiments, the inner tube element 111 is made of polycarbonate (PC).

[0093] In some of these embodiments, the inner tube element 111 is a collection tube.

[0094] The cross-section of the adsorption unit 120 is circular.

[0095] In some embodiments, the adsorption unit 120 includes an adsorption plate and a plurality of adsorption holes. The adsorption plate is disposed in the middle of the inner tube element 111 and connected to the inner tube element 111; the plurality of adsorption holes are respectively disposed through the adsorption plate and are used to filter the mixed liquid containing biological tissue under the action of a negative pressure device to retain the biological tissue, and to immerse the biological tissue in the lysis solution of the container device when the inner tube element 111 is disposed inside the container device.

[0096] The dimensions of the adsorption plate are matched with the dimensions of the inner tube element 111. Generally, the radial dimension of the adsorption plate is equal to the radial dimension of the inner edge surface of the inner tube element 111, and the axial dimension of the adsorption plate is smaller than the axial dimension of the inner tube element 111.

[0097] The dimensions of the adsorption pores are matched to the dimensions of the adsorption plate. Generally, the radial dimension of the adsorption pores is smaller than the radial dimension of the adsorption plate, and the axial dimension of the adsorption pores is equal to the axial dimension of the adsorption plate.

[0098] In some of these embodiments, the adsorption unit 120 is made of polycarbonate (PC).

[0099] like Figure 4 As shown, the outer tube unit 130 includes an outer tube element 131 and a first connecting element 132. The outer tube element 131 is disposed on the outer edge surface of the top end of the inner tube unit 110; the first connecting element 132 is disposed on the inner edge surface of the top end of the outer tube element 131 and is connected to the outer tube element 131 and the inner tube unit 110 respectively.

[0100] Specifically, the outer tube element 131 is disposed on the outer edge surface of the top end of the inner tube element 111; the first connecting element 132 is connected to the inner tube element 111.

[0101] The outer tube element 131 has a hollow structure.

[0102] The dimensions of the outer tube element 131 are matched with the dimensions of the inner tube element 111. Generally, the radial dimension (i.e., inner diameter) of the inner edge surface of the outer tube element 131 is larger than the radial dimension (i.e., outer diameter) of the outer edge surface of the inner tube element 111, and the axial dimension (length or height) of the outer tube element 131 is smaller than the axial dimension (length or height) of the inner tube element 111.

[0103] The dimensions of the outer tube element 131 are matched with the dimensions of the container assembly. Generally, the radial dimension (e.g., inner diameter) of the inner edge surface of the outer tube element 131 is not less than the radial dimension (e.g., outer diameter) of the outer edge surface of the container assembly, and the axial dimension (e.g., length or height) of the outer tube element 131 is less than the axial dimension (e.g., length or height) of the container assembly.

[0104] In some of these embodiments, the outer tube element 131 is made of polyethylene terephthalate (PET).

[0105] In some of these embodiments, the outer tube element 131 is an outer tube.

[0106] The first connecting element 132 has a hollow structure.

[0107] The dimensions of the first connecting element 132 are matched with the dimensions of the outer tube element 131. Generally, the radial dimension (e.g., outer diameter) of the outer edge surface of the first connecting element 132 is equal to the radial dimension (e.g., inner diameter) of the inner edge surface of the outer tube element 131, and the axial dimension (e.g., length or height) of the first connecting element 132 is smaller than the axial dimension (e.g., length or height) of the outer tube element 131.

[0108] The dimensions of the first connecting element 132 are matched with the dimensions of the inner tube element 111. Generally, the radial dimension (e.g., inner diameter) of the inner edge surface of the first connecting element 132 is equal to the radial dimension (e.g., outer diameter) of the outer edge surface of the inner tube element 111, and the axial dimension (e.g., length or height) of the first connecting element 132 is smaller than the axial dimension (e.g., length or height) of the inner tube element 111.

[0109] In some of the embodiments, the first connecting element 132 is fixedly connected to the outer tube element 131 and the inner tube element 111, respectively, including but not limited to thermofusion connection.

[0110] In some of these embodiments, the first connecting element 132 is made of polyethylene terephthalate (PET).

[0111] In some of these embodiments, the first connecting element 132 is a connecting pipe.

[0112] The method of using this utility model is as follows:

[0113] (I) Adsorption of tissue samples

[0114] Connect one end of the inner tube component 111 (the end furthest from the outer tube component 131) to the hose provided by the negative pressure device;

[0115] Place the other end of the inner tube component 111 into the mortar;

[0116] The negative pressure device is activated to generate negative pressure suction inside the inner tube element 111, thereby adsorbing the tissue sample onto the surface of the adsorption unit 120 provided inside the inner tube element 111.

[0117] During the process, if there is other liquid around the tissue sample, the liquid will enter the negative pressure device through the adsorption unit 120.

[0118] After adsorption, turn the other end of the inner tube element 111 upward to prevent the adsorbed tissue sample from being removed from the inner tube element 111, and turn off the negative pressure device.

[0119] (II) Lysed tissue samples

[0120] Separate the inner tube component 111 from the negative pressure device;

[0121] The inner tube element 111 is placed into the container device, and the outer tube element 131 is located outside the container device, so that the biological tissue collection device 100 is connected to the container device, so that the lysis solution in the container device enters the inner tube element 111 through the adsorption unit 120 and immerses the tissue sample in the inner tube element 111.

[0122] Seal the inner tube component 111;

[0123] After lysis is completed, the inner tube element 111 is separated from the container device, and the slightly larger tissue samples that are not completely lysed on the adsorption unit 120 are directly discarded.

[0124] (III) Centrifugation of pyrolysis solution

[0125] After placing the container containing the lysate into a centrifuge and performing a series of operations such as centrifugation, researchers can extract the desired components such as RNA and protein from the tissue sample.

[0126] The advantage of this invention is that the inner tube unit and the container device are detachable, so that they can be separated when adsorbing tissue samples, allowing them to be used independently. The tissue sample can be adsorbed onto the adsorption unit by the cooperation between the adsorption unit and the inner tube unit, which replaces the repeated use of the spatula and avoids the tissue sample from being poured in by the spatula and spilling, thus ensuring the reliability of tissue sample transfer.

[0127] Example 2

[0128] This embodiment is a modified embodiment of embodiment 1.

[0129] The first implementation of this embodiment: the inner tube unit 110 is threadedly connected to the container device.

[0130] like Figure 3As shown, the inner tube unit 110 also includes a second connecting element 112. The second connecting element 112 is disposed at the top end of the inner tube unit 111 and is detachably connected to the container device.

[0131] The dimensions of the second connecting element 112 are matched with the dimensions of the inner tube element 111. Generally, the axial dimension of the second connecting element 112 is smaller than the axial dimension of the inner tube element 111.

[0132] In some of these embodiments, the second connecting element 112 is a first threaded groove.

[0133] The second implementation of this embodiment: the outer tube unit 130 is threadedly connected to the container device.

[0134] like Figure 4 As shown, the outer tube unit 130 also includes a third connecting element 133. The third connecting element 133 is disposed on the inner edge surface of the outer tube element 131 and is located below the first connecting element 132, and is detachably connected to the container device.

[0135] The dimensions of the third connecting element 133 are matched with the dimensions of the outer tube element 131. Generally, the axial dimension of the third connecting element 133 is smaller than the axial dimension of the outer tube element 131.

[0136] In some of these embodiments, the third connecting element 133 is a second threaded groove.

[0137] The method of using this utility model is as follows:

[0138] (I) Adsorption of tissue samples

[0139] Same as step (1) of Example 1.

[0140] (II) Lysed tissue samples

[0141] Separate the inner tube component 111 from the negative pressure device;

[0142] The inner tube element 111, which adsorbs tissue samples, is threadedly connected to the container device via the second connecting element 112, or the outer tube element 131 is threadedly connected to the container device via the third connecting element 133.

[0143] The lysis buffer inside the container device enters the inner tube element 111 through the adsorption unit 120, immersing the tissue sample in the lysis buffer so that substances such as RNA in the tissue sample are lysed in the lysis buffer.

[0144] After lysis is complete, twist the inner tube element 111 until it separates from the container device, remove the inner tube element 111 from the container device, and discard the slightly larger tissue samples that are not completely lysed on the adsorption unit 120.

[0145] (III) Centrifugation of pyrolysis solution

[0146] Same as step (iii) of Example 1.

[0147] The advantage of this embodiment is that the inner tube unit and the container device are detachable, so that they can be separated when adsorbing tissue samples, and thus used separately.

[0148] Example 3

[0149] This embodiment is a modified embodiment of Embodiments 1 and 2.

[0150] like Figure 5 , Figure 6 , Figure 7 As shown, the biological tissue collection device 100 also includes a sealing unit 140. The sealing unit 140 is removably disposed on the top of the inner tube unit 110 for sealing the inner tube unit 110.

[0151] In the first implementation of this embodiment, the outer tube unit 130 and the sealing unit 140 are detachably connected.

[0152] like Figure 8 As shown, the outer tube unit 130 also includes a fourth connecting element 134. The fourth connecting element 134 is disposed on the outer edge surface of the outer tube unit 130 and is detachably connected to the sealing unit 140.

[0153] Specifically, the fourth connecting element 134 is disposed on the outer edge surface of the outer tube element 131.

[0154] The dimensions of the fourth connecting element 134 are matched with the dimensions of the outer tube element 131. Generally, the axial dimension of the fourth connecting element 134 is not greater than the axial dimension of the outer tube element 131.

[0155] In some of these embodiments, the fourth connecting element 134 is a third threaded groove.

[0156] like Figure 9 As shown, the sealing unit 140 includes a sealing element 141 and a fifth connecting element 142. The sealing element 141 is removably disposed on the top of the inner tube unit 110 for sealing the inner tube unit 110; the fifth connecting element 142 is disposed on the inner edge surface of the sealing element 141 and is detachably connected to the outer tube unit 130.

[0157] Specifically, the sealing element 141 is removably disposed on the top of the inner tube element 111; the fifth connecting element 142 is detachably connected to the fourth connecting element 134.

[0158] The sealing element 141 has a closed top and a hollow bottom structure.

[0159] The dimensions of the sealing element 141 are matched with the dimensions of the outer tube element 131. Generally, the radial dimension (e.g., inner diameter) of the inner edge surface of the sealing element 141 is equal to the radial dimension (e.g., outer diameter) of the outer edge surface of the outer tube element 131, and the inner axial dimension of the sealing element 141 is not less than the axial dimension of the outer tube element 131.

[0160] In some of these embodiments, the sealing element 141 is made of polyethylene terephthalate (PET).

[0161] In some of these embodiments, the sealing element 141 is a closure cap.

[0162] The dimensions of the fifth connecting element 142 are matched with the dimensions of the sealing element 141. Generally, the axial dimension of the fifth connecting element 142 is equal to the axial dimension of the inner side of the sealing element 141.

[0163] The dimensions of the fifth connecting element 142 match those of the fourth connecting element 134. Generally, the axial dimension of the fifth connecting element 142 is equal to the axial dimension of the fourth connecting element 134.

[0164] In some of these embodiments, the fifth connecting element 142 is a second threaded tooth.

[0165] The second embodiment of this example: the inner tube unit 110 and the sealing unit 140 are detachably connected.

[0166] The sealing unit 140 includes a sealing element 141 and a fifth connecting element 142. The sealing element 141 is removably disposed on the top of the inner tube unit 110 for sealing the inner tube unit 110; the fifth connecting element 142 is disposed on the bottom of the sealing element 141 and is detachably connected to the inner tube unit 110.

[0167] Specifically, the sealing element 141 is removably disposed on the top of the inner tube element 111; the fifth connecting element 142 is detachably connected to the inner tube element 111.

[0168] The sealing element 141 has a circular cross-section.

[0169] The dimensions of the sealing element 141 are matched with the dimensions of the inner tube element 111. Generally, the radial dimension (e.g., diameter) of the sealing element 141 is larger than the radial dimension (e.g., outer diameter) of the outer edge of the inner tube element 111, and the axial dimension (e.g., length or height) of the sealing element 141 is smaller than the axial dimension (e.g., length or height) of the inner tube element 111.

[0170] In some of these embodiments, the sealing element 141 is made of polyethylene terephthalate (PET).

[0171] In some of these embodiments, the sealing element 141 is a closure cap.

[0172] The fifth connecting element 142 has a circular cross-section.

[0173] The dimensions of the fifth connecting element 142 are matched with the dimensions of the sealing element 141. Generally, the radial dimension (e.g., diameter) of the fifth connecting element 142 is smaller than the radial dimension (e.g., diameter) of the sealing element 141, and the axial dimension (e.g., depth) of the fifth connecting element 142 is smaller than the radial dimension (e.g., length or height) of the sealing element 141.

[0174] The dimensions of the fifth connecting element 142 are matched with the dimensions of the inner tube element 111. Generally, the radial dimension (e.g., diameter) of the fifth connecting element 142 is equal to the radial dimension (e.g., outer diameter) of the outer edge of the inner tube element 111, and the axial dimension (e.g., depth) of the fifth connecting element 142 is smaller than the axial dimension (e.g., length or height) of the inner tube element 111.

[0175] In some of these embodiments, the fifth connecting element 142 is a plug-in hole.

[0176] The method of using this utility model is as follows:

[0177] (I) Adsorption of tissue samples

[0178] Same as step (1) of Example 1.

[0179] (II) Lysed tissue samples

[0180] Separate the inner tube component 111 from the negative pressure device;

[0181] The inner tube element 111, which adsorbs tissue samples, is threadedly connected to the container device via the second connecting element 112, or the outer tube element 131 is threadedly connected to the container device via the third connecting element 133.

[0182] The lysis buffer inside the container device enters the inner tube element 111 through the adsorption unit 120, immersing the tissue sample in the lysis buffer to lyse substances such as RNA in the tissue sample.

[0183] The sealing element 141 is threadedly connected to the fourth connecting element 134 of the outer tube element 131 via the fifth connecting element 142, or the sealing element 141 is inserted into the top end of the inner tube element 111 via the fifth connecting element 142 to seal the inner tube element 111.

[0184] After lysis is complete, twist the inner tube element 111 until it separates from the container device, remove the inner tube element 111 from the container device, and discard the slightly larger tissue samples that are not completely lysed on the adsorption unit 120.

[0185] (III) Centrifugation of pyrolysis solution

[0186] Same as step (iii) of Example 1.

[0187] The advantage of this embodiment is that the sealing unit is used to seal the inner tube unit, which prevents the tissue sample inside the inner tube unit from moving out and ensures the airtightness of the inner tube unit.

[0188] Example 4

[0189] This embodiment is a modified embodiment of Embodiments 1 to 3.

[0190] like Figure 5 , Figure 6 , Figure 7 As shown, the biological tissue collection device 100 also includes a transfer unit 150. The first end of the transfer unit 150 is detachably connected to the inner tube unit 110 or the outer tube unit 130, and the second end of the transfer unit 150 is detachably connected to the negative pressure device.

[0191] like Figure 10 As shown, the inner tube unit 110 also includes a sixth connecting element 113. The sixth connecting element 113 is disposed on the outer edge surface of the bottom end of the inner tube unit 110 and is detachably connected to the first end of the adapter unit 150.

[0192] Specifically, the sixth connecting element 113 is disposed on the outer edge surface of the bottom end of the inner tube element 111.

[0193] The dimensions of the sixth connecting element 113 are matched with the dimensions of the inner tube element 111. Generally, the axial dimension of the sixth connecting element 113 is smaller than the axial dimension of the inner tube element 111.

[0194] In some of these embodiments, the sixth connecting element 113 is a fourth threaded groove.

[0195] like Figure 8 As shown, the outer tube unit 130 also includes a fourth connecting element 134. The fourth connecting element 134 is disposed on the outer edge surface of the outer tube unit 130 and is detachably connected to the first end of the adapter unit 150.

[0196] Specifically, the fourth connecting element 134 is disposed on the outer edge surface of the outer tube element 131.

[0197] The dimensions of the fourth connecting element 134 are matched with the dimensions of the outer tube element 131. Generally, the axial dimension of the fourth connecting element 134 is equal to the axial dimension of the outer tube element 131.

[0198] In some of these embodiments, the fourth connecting element 134 is a third threaded groove.

[0199] like Figure 11 As shown, the adapter unit 150 includes an adapter element 151, a seventh connecting element 152, and an eighth connecting element 153. The seventh connecting element 152 is disposed at the first end of the adapter element 151 and is detachably connected to the inner tube unit 110 or the outer tube unit 130; the eighth connecting element 153 is disposed at the second end of the adapter element 151 and is detachably connected to the negative pressure device.

[0200] Specifically, the seventh connecting element 152 is detachably connected to the sixth connecting element 113 or the fourth connecting element 134.

[0201] The adapter element 151 has a hollow structure.

[0202] The adapter element 151 has a circular upper part and a conical lower part. Specifically, the adapter element 151 includes an upper adapter tube and a lower adapter tube. The first end of the upper adapter tube is provided with a seventh connecting element 152; the lower adapter tube is provided at the second end of the upper adapter tube, and the second end of the lower adapter tube is provided with an eighth connecting element 153.

[0203] In some embodiments, the radial dimension of the lower connector decreases from its top end to its bottom end. That is, the radial dimension of the lower connector decreases from the end closer to the upper connector to the end farther away from the upper connector.

[0204] The top adapter pipe comes in two sizes. Details are as follows:

[0205] 1. The dimensions of the upper adapter pipe match the dimensions of the inner tube element 111. Generally, the radial dimension of the inner edge surface of the upper adapter pipe is equal to the radial dimension of the outer edge surface of the inner tube element 111, and the axial dimension of the upper adapter pipe is smaller than the axial dimension of the inner tube element 111.

[0206] 2. The dimensions of the upper adapter pipe match the dimensions of the outer pipe element 131. Generally, the radial dimension of the inner edge surface of the upper adapter pipe is equal to the radial dimension of the outer edge surface of the outer pipe element 131, and the axial dimension of the upper adapter pipe is greater than the axial dimension of the outer pipe element 131.

[0207] The dimensions of the lower connecting pipe match those of the upper connecting pipe. Generally, the radial dimension of the lower connecting pipe (such as the radial dimension of the maximum outer edge surface and the radial dimension of the maximum inner edge surface) is equal to the radial dimension of the upper connecting pipe (such as the radial dimension of the outer edge surface and the radial dimension of the inner edge surface of the upper connecting pipe), and the axial dimension of the lower connecting pipe is equal to the axial dimension of the upper connecting pipe.

[0208] In some of these embodiments, the adapter element 151 is made of polyethylene terephthalate (PET).

[0209] The dimensions of the seventh connecting element 152 are matched with the dimensions of the upper adapter pipe. Generally, the axial dimension of the seventh connecting element 152 is not greater than the axial dimension of the upper adapter pipe.

[0210] The seventh connecting element 152 comes in two sizes. Details are as follows:

[0211] 1. The dimensions of the upper adapter pipe match the dimensions of the inner pipe component 111.

[0212] The dimensions of the seventh connecting element 152 are matched with those of the sixth connecting element 113. Generally, the axial dimension of the seventh connecting element 152 is not less than the axial dimension of the sixth connecting element 113.

[0213] 2. The dimensions of the upper adapter pipe match the dimensions of the outer pipe component 131.

[0214] The dimensions of the seventh connecting element 152 are matched with those of the fourth connecting element 134. Generally, the axial dimension of the seventh connecting element 152 is not less than the axial dimension of the fourth connecting element 134.

[0215] In some of these embodiments, the seventh connecting element 152 is a second threaded tooth.

[0216] The eighth connecting element 153 has a circular cross-section and a circular arc longitudinal section.

[0217] The dimensions of the eighth connecting element 153 are matched with the dimensions of the lower connecting pipe. Generally, the radial dimension of the inner edge surface of the eighth connecting element 153 is equal to the radial dimension of the outer edge surface of the lower connecting pipe, and the axial dimension of the eighth connecting element 153 is smaller than the axial dimension of the lower connecting pipe.

[0218] In some embodiments, there are multiple eighth connecting elements 153. These multiple eighth connecting elements 153 are spaced apart along the axial direction of the lower connecting pipe.

[0219] In some embodiments, the eighth connecting element 153 is fixedly connected to the adapter element 151, including but not limited to being integrally formed.

[0220] In some of these embodiments, the eighth connecting element 153 is made of polyethylene terephthalate (PET).

[0221] In some of these embodiments, the eighth connecting element 153 is a bump or a third threaded tooth.

[0222] The method of using this utility model is as follows:

[0223] (I) Adsorption of tissue samples

[0224] Connect the adapter 151 to the sixth connecting element 113 of the inner tube element 111 via the seventh connecting element 152, or connect the adapter 151 to the fourth connecting element 134 of the outer tube element 131 via the seventh connecting element 152, until tightened and fixed.

[0225] The negative pressure device is connected to the eighth connecting element 153 provided in the adapter element 151 via a hose;

[0226] Place one end of the inner tube element 111 (the end furthest from the adapter element 151) into the mortar;

[0227] The negative pressure device is activated so that negative pressure suction is generated in the inner tube element 111 through the adapter element 151, thereby adsorbing the tissue sample onto the surface of the adsorption unit 120 provided in the inner tube element 111.

[0228] During the process, if there is other liquid around the tissue sample, the liquid will enter the negative pressure device through the adsorption unit 120.

[0229] After adsorption, one end of the inner tube element 111 is turned upwards to prevent the adsorbed tissue sample from being removed from the inner tube element 111, and the negative pressure device is turned off.

[0230] (II) Lysed tissue samples

[0231] Same as step (ii) of Example 1.

[0232] (III) Centrifugation of pyrolysis solution

[0233] Same as step (iii) of Example 1.

[0234] The advantage of this invention is that it can be connected to pipes of negative pressure devices of different sizes by using the adapter unit, which improves the applicability of the assembly.

[0235] Example 5

[0236] This embodiment is a modified embodiment of Embodiments 1 to 4.

[0237] like Figure 5 , Figure 6 , Figure 7As shown, the biological tissue collection device 100 also includes a container unit 160. The container unit 160 has an inner tube unit 110 removably disposed inside, and is detachably connected to either the inner tube unit 110 or the outer tube unit 130, for storing lysis buffer.

[0238] like Figure 12 As shown, container unit 160 includes container element 161. An inner tube unit 110 is removably disposed inside container element 161 and is detachably connected to either the inner tube unit 110 or the outer tube unit 130 for storing pyrolysis fluid.

[0239] The container element 161 has an inner tube element 111 removably disposed inside and is detachably connected to the inner tube element 111 or the outer tube element 131.

[0240] The container element 161 has a hollow top and a closed bottom structure.

[0241] The container element 161 has a circular upper part and a conical lower part. Specifically, the container element 161 includes a circular tube and a conical tube. The inner tube element 111 is removably disposed inside the circular tube and is detachably connected to the inner tube element 111 or the outer tube element 131; the conical tube is disposed at the bottom end of the circular tube and communicates with the circular tube.

[0242] In some embodiments, the radial dimension of the conical tube decreases from its apex to its base. That is, the radial dimension of the conical tube decreases from the end closer to the tube to the end further away from the tube.

[0243] The dimensions of the circular tube body match the dimensions of the inner tube element 111. Generally, the radial dimension of the inner edge surface of the circular tube body is equal to the radial dimension of the outer edge surface of the inner tube element 111, and the axial dimension of the circular tube body is not less than the axial dimension of the inner tube element 111.

[0244] The dimensions of the circular tube body are matched with the dimensions of the outer tube element 131. Generally, the radial dimension of the outer edge surface of the circular tube body is equal to the radial dimension of the inner edge surface of the outer tube element 131, and the axial dimension of the circular tube body is greater than the axial dimension of the outer tube element 131.

[0245] The dimensions of the conical tube are matched with those of the circular tube. Generally, the radial dimensions of the conical tube (such as the maximum outer edge and the maximum inner edge) are equal to the radial dimensions of the circular tube (such as the outer edge and the inner edge), and the axial dimensions of the conical tube are smaller than the axial dimensions of the circular tube.

[0246] In some of these embodiments, container element 161 is made of polycarbonate (PC).

[0247] In some of these embodiments, container element 161 is a centrifuge tube.

[0248] Furthermore, the container unit 160 also includes a ninth connecting element 162. The ninth connecting element 162 is disposed at the top of the container unit 161 and is detachably connected to the inner tube unit 110 or the outer tube unit 130.

[0249] Specifically, the ninth connecting element 162 is detachably connected to the second connecting element 112 or the third connecting element 133.

[0250] In the first embodiment of this example: when the ninth connecting element 162 is detachably connected to the second connecting element 112, the ninth connecting element 162 is disposed on the inner edge surface of the top of the container element 161.

[0251] The dimensions of the ninth connecting element 162 are matched with the dimensions of the circular tube. Generally, the axial dimension of the ninth connecting element 162 is smaller than the axial dimension of the circular tube.

[0252] The dimensions of the ninth connecting element 162 are matched with those of the second connecting element 112. Generally, the axial dimension of the ninth connecting element 162 is not less than the axial dimension of the second connecting element 112.

[0253] The second implementation of this embodiment: When the ninth connecting element 162 is detachably connected to the third connecting element 133, the ninth connecting element 162 is disposed on the outer edge surface of the top of the container element 161.

[0254] The dimensions of the ninth connecting element 162 are matched with the dimensions of the circular tube. Generally, the axial dimension of the ninth connecting element 162 is smaller than the axial dimension of the circular tube.

[0255] The dimensions of the ninth connecting element 162 are matched with those of the third connecting element 133. Generally, the axial dimension of the ninth connecting element 162 is not less than the axial dimension of the third connecting element 133.

[0256] In some of these embodiments, the ninth connecting element 162 is the fourth threaded tooth.

[0257] Furthermore, the container unit 160 also includes a marking element 163. The marking element 163 is disposed on the outer edge surface of the container unit 161 and is used to indicate the volume of the lysis solution inside the container unit 161.

[0258] The dimensions of the marking element 163 are matched with the dimensions of the circular tube. Generally, the axial dimension of the marking element 163 is smaller than the axial dimension of the circular tube.

[0259] In some of these embodiments, the marking element 163 is a scale line.

[0260] The method of using this utility model is as follows:

[0261] (I) Adsorption of tissue samples

[0262] Same as step (1) of Example 1.

[0263] (II) Lysed tissue samples

[0264] Separate the inner tube component 111 from the negative pressure device;

[0265] The inner tube element 111, which adsorbs tissue samples, is threadedly connected to the ninth connecting element 162 of the container element 161 via the second connecting element 112, or the inner tube element 111 is threadedly connected to the ninth connecting element 162 of the container element 161 via the third connecting element 133 of the outer tube element 131.

[0266] The lysis buffer inside container element 161 enters inner tube element 111 through adsorption unit 120, immersing the tissue sample in the lysis buffer to lyse RNA and other substances in the tissue sample.

[0267] The sealing element 141 is threadedly connected to the fourth connecting element 134 of the outer tube element 131 via the fifth connecting element 142, or the sealing element 141 is inserted into the top end of the inner tube element 111 via the fifth connecting element 142 to seal the inner tube element 111.

[0268] After lysis is complete, twist the inner tube element 111 until it separates from the container element 161, remove the inner tube element 111 from the container element 161, and discard any slightly larger tissue samples that are not completely lysed on the adsorption unit 120.

[0269] (III) Centrifugation of pyrolysis solution

[0270] Same as step (iii) of Example 1.

[0271] The advantage of this embodiment is that the inner tube unit and the container unit are detachable, so that they can be separated when adsorbing tissue samples, and thus used separately.

[0272] Example 6

[0273] This embodiment is a modified embodiment of Embodiments 1 to 5.

[0274] like Figure 7 As shown, the biological tissue collection device 100 also includes a delivery unit 170, the first end of which is detachably connected to the inner tube unit 110, and the second end of which is detachably connected to the negative pressure device.

[0275] Specifically, the conveying unit 170 is detachably connected to the inner tube element 111, the outer tube element 131, or the adapter element 151 (lower adapter).

[0276] In the first implementation of this embodiment, the conveying unit 170 is detachably connected to the inner tube element 111.

[0277] The dimensions of the conveying unit 170 are matched with the dimensions of the inner tube element 111. Generally, the radial dimension of the inner edge surface of the conveying unit 170 is equal to the radial dimension of the outer edge surface of the inner tube element 111, and the axial dimension of the conveying unit 170 is greater than the axial dimension of the inner tube element 111.

[0278] The second implementation of this embodiment: the conveying unit 170 is detachably connected to the outer tube element 131.

[0279] The dimensions of the conveying unit 170 are matched with the dimensions of the outer tube element 131. Generally, the radial dimension of the inner edge surface of the conveying unit 170 is equal to the radial dimension of the outer edge surface of the outer tube element 131, and the axial dimension of the conveying unit 170 is greater than the axial dimension of the outer tube element 131.

[0280] The third embodiment of this example: the conveying unit 170 is detachably connected to the adapter element 151.

[0281] The dimensions of the conveying unit 170 are matched with the dimensions of the adapter element 151. Generally, the radial dimension of the inner edge surface of the conveying unit 170 is equal to the radial dimension of the outer edge surface of the lower adapter, and the axial dimension of the conveying unit 170 is greater than the axial dimension of the lower adapter.

[0282] In some of these embodiments, the conveying unit 170 is made of silicone.

[0283] In some of these embodiments, the delivery unit 170 is a flexible hose.

[0284] The method of using this utility model is as follows:

[0285] (I) Adsorption of tissue samples

[0286] One end of the conveying unit 170 is inserted into the inner tube element 111, the outer tube element 131, or the adapter element 151.

[0287] Connect the other end of the conveying unit 170 to the negative pressure device;

[0288] When one end of the conveying unit 170 is inserted into the inner tube element 111, the other end of the inner tube element 111 (the end away from the conveying unit 170) is placed in the mortar.

[0289] When one end of the conveying unit 170 is inserted into the outer tube element 131, one end of the inner tube element 111 (the end away from the outer tube element 131) is placed in the mortar.

[0290] When one end of the conveying unit 170 is plugged into the adapter element 151, one end of the inner tube element 111 (the end away from the adapter element 151) is placed in the mortar.

[0291] The negative pressure device is activated to generate negative pressure suction inside the inner tube element 111, thereby adsorbing the tissue sample onto the surface of the adsorption unit 120 provided inside the inner tube element 111.

[0292] During the process, if there is other liquid around the tissue sample, the liquid will enter the negative pressure device through the adsorption unit 120.

[0293] After adsorption, one end of the inner tube element 111 (the end placed in the mortar) is turned upwards to prevent the adsorbed tissue sample from being removed from the inner tube element 111, and the negative pressure device is turned off.

[0294] (II) Lysed tissue samples

[0295] Same as step (ii) of Example 1.

[0296] (III) Centrifugation of pyrolysis solution

[0297] Same as step (iii) of Example 1.

[0298] The advantage of this embodiment is that by using the conveying unit in conjunction, the inner tube unit and the negative pressure device can be connected by the conveying unit as an adapter, thereby diversifying the connection between the two.

[0299] Example 7

[0300] This embodiment relates to the collection system of this utility model.

[0301] like Figure 13 As shown, a biological tissue collection system includes a biological tissue collection device 100 as described in any one of Examples 1 to 6 and a negative pressure device 200. The negative pressure device 200 is detachably connected to the inner tube unit 110 of the biological tissue collection device 100.

[0302] Specifically, the negative pressure device 200 is detachably connected to the inner tube element 111, the outer tube element 131, or the adapter element 151, and communicates with the inner tube element 111, the outer tube element 131, or the adapter element 151.

[0303] In some of these embodiments, the negative pressure device 200 is a suction device.

[0304] Furthermore, the biological tissue collection system also includes a grinding device 300. The grinding device 300 has the top end of the inner tube unit 110 of the biological tissue collection device 100 removably disposed inside, for grinding biological tissue in a liquid nitrogen environment.

[0305] Specifically, the top end of the inner tube element 111 or the top end of the outer tube element 131 is removably provided inside the grinding device 300.

[0306] In some embodiments, the grinding apparatus 300 includes a mortar and a pestle. The mortar has a removable top end of an inner tube element 111 or a top end of an outer tube element 131 for holding a tissue sample; the pestle is used to strike the tissue sample inside the mortar.

[0307] Furthermore, the biological tissue collection system also includes a centrifuge device 400. The centrifuge device 400 has a removable container unit 160 of the container device or biological tissue collection device 100 for centrifugation to separate biological tissue and lysate.

[0308] Specifically, a container element 161 is removably disposed inside the centrifuge device 400.

[0309] In some of these embodiments, the centrifugation device 400 is a centrifuge.

[0310] The usage method of this embodiment is basically the same as that of Embodiments 1 to 6, and will not be repeated here.

[0311] The above description is only a preferred embodiment of the present utility model and does not limit the implementation method and protection scope of the present utility model. Those skilled in the art should realize that all solutions obtained by equivalent substitutions and obvious changes made based on the description and illustrations of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A biological tissue collection device, characterized in that, include: An inner tube unit (110) is removably disposed inside the container device and detachably connected to a negative pressure device for adsorbing a mixed liquid containing biological tissue under the action of the negative pressure device. An adsorption unit (120) is disposed at the bottom of the inner tube unit (110) for filtering a mixed liquid containing biological tissue under the action of a negative pressure device to retain the biological tissue and for immersing the biological tissue in the lysate of the container device when the inner tube unit (110) is disposed inside the container device. An outer tube unit (130) is disposed on top of the inner tube unit (110) and is detachably connected to the container device.

2. The biological tissue collection device according to claim 1, characterized in that, The inner tube unit (110) includes: An inner tube element (111), the bottom end of which is connected to the adsorption unit (120), and the top end of which is connected to the outer tube unit (130), the inner tube element (111) is removably disposed inside the container device and detachably connected to the negative pressure device, for adsorbing mixed liquids containing biological tissue under the action of the negative pressure device; and / or The outer tube unit (130) includes: An outer tube element (131) is disposed on the outer edge surface of the top end of the inner tube unit (110); A first connecting element (132) is disposed on the inner edge surface of the top end of the outer tube element (131) and is connected to the outer tube element (131) and the inner tube unit (110) respectively.

3. The biological tissue collection device according to claim 2, characterized in that, The inner tube unit (110) also includes: A second connecting element (112) is disposed at the top end of the inner tube element (111) and is detachably connected to the container device; and / or The outer tube unit (130) also includes: A third connecting element (133) is disposed on the inner edge surface of the outer tube element (131) and located below the first connecting element (132), and is detachably connected to the container device.

4. The biological tissue collection device according to any one of claims 1 to 3, characterized in that, Also includes: A sealing unit (140), removably disposed on top of the inner tube unit (110), for sealing the inner tube unit (110); and / or A connecting unit (150), the first end of which is detachably connected to the inner tube unit (110) or the outer tube unit (130), and the second end of which is detachably connected to a negative pressure device; and / or A container unit (160), wherein the inner tube unit (110) is removably disposed inside the container unit (160) and is detachably connected to the inner tube unit (110) or the outer tube unit (130), for storing pyrolysis fluid; and / or The conveying unit (170) has a first end that is detachably connected to the inner tube unit (110) and a second end that is detachably connected to the negative pressure device.

5. The biological tissue collection device according to claim 4, characterized in that, The outer tube unit (130) also includes: A fourth connecting element (134) is disposed on the outer edge surface of the outer tube unit (130) and is detachably connected to the sealing unit (140); and / or The sealing unit (140) includes: A sealing element (141) is removably disposed on the top of the inner tube unit (110) for sealing the inner tube unit (110); The fifth connecting element (142) is disposed at the bottom end or inner edge of the sealing element (141) and is detachably connected to the inner tube unit (110) or the outer tube unit (130).

6. The biological tissue collection device according to claim 4, characterized in that, The inner tube unit (110) also includes: A sixth connecting element (113) is disposed on the outer edge of the bottom end of the inner tube unit (110) and is detachably connected to the first end of the adapter unit (150); and / or The outer tube unit (130) also includes: A fourth connecting element (134) is disposed on the outer edge surface of the outer tube unit (130) and is detachably connected to the first end of the adapter unit (150); and / or The adapter unit (150) includes: Adapter element (151); A seventh connecting element (152) is disposed at the first end of the adapter element (151) and is detachably connected to the inner tube unit (110) or the outer tube unit (130). The eighth connecting element (153) is disposed at the second end of the adapter element (151) and is detachably connected to the negative pressure device.

7. The biological tissue collection device according to claim 4, characterized in that, The container unit (160) includes: A container element (161) having the inner tube unit (110) removably disposed inside and being detachably connected to the inner tube unit (110) or the outer tube unit (130) for storing pyrolysis fluid.

8. The biological tissue collection device according to claim 7, characterized in that, The container unit (160) further includes: A ninth connecting element (162), wherein the ninth connecting element (162) is disposed at the top of the container element (161) and is detachably connected to the inner tube unit (110) or the outer tube unit (130); and / or A marking element (163) is disposed on the outer edge of the container element (161) for indicating the volume of the lysate inside the container element (161).

9. A biological tissue collection system, characterized in that, include: The biological tissue collection device (100) as described in any one of claims 1 to 8; A negative pressure device (200) is detachably connected to the inner tube unit (110) of the biological tissue collection device (100).

10. The biological tissue collection system according to claim 9, characterized in that, Also includes: A grinding device (300), wherein the top end of the inner tube unit (110) of the biological tissue collection device (100) is removably disposed inside the grinding device (300) for grinding biological tissue in a liquid nitrogen environment; and / or A centrifuge device (400) having a container device or the container unit (160) of the biological tissue collection device (100) removably disposed inside the centrifuge device (400) for centrifuging to separate biological tissue and lysate.

Images