Rapid incubator for culturing mesenchymal stem cells

Abstract

The utility model provides a rapid incubator for culturing mesenchymal stem cells, which relates to the technical field of cell culture and comprises an incubator body, a mounting groove is arranged at the bottom of the inside of the incubator body, an auxiliary heating component is mounted in the mounting groove, a pure water placing area is arranged in the inner wall of the incubator body, and the pure water placing area is communicated with the mounting groove. A pure water containing area is arranged in the cultivation box body, a heating wire is arranged in the pure water containing area, a first heating controller is installed at the position, located on the left side wall of the cultivation box body, of the joint of the heating wire, and a humidifying assembly is arranged at the top of the cultivation box body. The temperature in the incubator body can be always in a stable and uniform state, meanwhile, through the design of the auxiliary heating assembly and the humidifying assembly, the incubator has the advantages of being capable of rapidly increasing the temperature, assisting in drying and keeping humidity, and it is ensured that various physiological processes in cells are normally carried out.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, and in particular to a rapid culture box for mesenchymal stem cell culture. Background Technology

[0002] In the field of mesenchymal stem cell culture, with the continuous development of stem cell research and applications, the requirements for precise control of its culture conditions are becoming increasingly stringent. Mesenchymal stem cells possess important functions such as multi-lineage differentiation potential and immune regulation, and have enormous application potential in multiple fields such as regenerative medicine and immunotherapy.

[0003] Traditional cell culture methods often fail to meet the specific needs of mesenchymal stem cells. During cell culture, environmental factors play a crucial role in cell growth, proliferation, and differentiation. As for temperature, the normal physiological activities of mesenchymal stem cells depend on a stable temperature environment, just like in the human body, a natural "incubator," where cells grow stably at around 37°C.

[0004] However, ordinary culture equipment may cause temperature fluctuations due to uneven heat dissipation and imperfect temperature control systems. These fluctuations can interfere with the complex enzymatic reactions, substance transport, and signal transduction processes within cells. For example, when the temperature is too high, proteins in the cells may denature and enzymes may lose their activity, thereby affecting the cell's metabolic function and even leading to cell death in severe cases. When the temperature is too low, the metabolic rate of the cells will be significantly reduced, slowing down cell growth and proliferation, which will greatly affect the culture efficiency and quality.

[0005] Humidity is also a crucial factor. Maintaining the moisture balance of the culture medium is essential during cell culture. Insufficient humidity in the culture environment leads to rapid water loss from the medium. This not only alters the concentration and osmotic pressure of the medium, causing osmotic damage to the cells, but also disrupts the microenvironment surrounding the cells. Cells require a suitable humidity environment to ensure a stable supply of water and nutrients to their surface, thereby maintaining normal material exchange and information transmission processes.

[0006] Therefore, we propose a rapid culture box for mesenchymal stem cell culture. Utility Model Content

[0007] The purpose of this invention is to address the shortcomings of existing technologies. Traditional cell culture methods are insufficient to meet the specific needs of mesenchymal stem cells. Environmental factors are crucial for their growth, proliferation, and differentiation. Temperature fluctuations can interfere with intracellular processes and affect metabolic function; both excessively high and low temperatures are harmful. Insufficient humidity can lead to water loss in the culture medium, altering its composition and osmotic pressure, disrupting the microenvironment, and affecting substance exchange.

[0008] To achieve the above objectives, the present invention adopts the following technical solution:

[0009] A rapid culture box for mesenchymal stem cell culture includes a culture box body. A mounting groove is provided at the bottom of the culture box body, and an auxiliary heating component is installed inside the mounting groove. A pure water placement area is provided in the inner wall of the culture box body, and a heating wire is provided in the pure water placement area. A first heating controller is installed at the connection point of the heating wire on the left side wall of the culture box body. A humidification component is provided at the top of the culture box body. Several temperature sensors are installed inside the culture box body on the left side, and a humidity sensor is installed inside the culture box body on the right side.

[0010] As a preferred embodiment of this utility model, the interior of the cultivation box is further provided with several placement plates, and several ventilation holes are opened between the placement plates and the bottom of the cultivation box. The interior of the pure water placement area is filled with water.

[0011] As a preferred embodiment of this utility model, the auxiliary heating component includes two fans, each with a filter screen below it, and several heating rods above the two fans. A second heating controller is provided on the left side of each of the heating rods.

[0012] In a preferred embodiment of this utility model, the fan and the filter are used in conjunction with each other, and the heating rod and the heating wire are electrically connected.

[0013] As a preferred embodiment of this utility model, the front of the cultivation box is provided with an installation sealing groove, the inside of the installation sealing groove is provided with a door panel, the door panel is provided with a pull groove, and the door panel is inserted into the installation sealing groove for use, thereby achieving a seal.

[0014] As a preferred embodiment of this utility model, the humidification component includes a connecting seat, the bottom of which is provided with a plurality of spray nozzles, and the top of which is provided with a connecting pipe.

[0015] As a preferred embodiment of this utility model, the humidification component further includes a water storage tank, which is fixedly installed on the back side of the cultivation box. The water storage tank is equipped with a water pump, which is connected to a connecting pipe.

[0016] In a preferred embodiment of this utility model, the humidification component and the humidity sensor are used in conjunction with each other, and the auxiliary heating component and the heating wire are used in conjunction with the temperature sensor. The humidification component, the auxiliary heating component, the humidity sensor, the temperature sensor and the heating wire are all connected to the PLC control computer.

[0017] Compared with the prior art, the beneficial effects of this utility model are:

[0018] In this invention, a pure water placement area is set up inside the incubator, and the water is heated by a heating wire, thus ensuring that the temperature inside the incubator remains stable and uniform. Simultaneously, the design of auxiliary heating and humidification components allows the incubator to rapidly heat up, assist in drying, and maintain humidity. This ensures the normal progress of various physiological processes within the cells, such as enzyme-catalyzed reactions proceeding at the optimal rate, guaranteeing a balance between synthesis and decomposition metabolism. Furthermore, its constant humidity function effectively prevents water loss from the culture medium. By maintaining suitable humidity, the osmotic pressure of the culture medium remains stable, avoiding damage to cells caused by osmotic pressure changes due to water evaporation. This creates a stable microenvironment for the cells, facilitating a continuous supply of water and nutrients to the cell surface, ensuring efficient material exchange and information transmission between the cells and the external environment, and maintaining normal cellular metabolic activities. Attached Figure Description

[0019] Figure 1 A schematic diagram of the main structure of the rapid culture box for mesenchymal stem cell culture provided by this utility model;

[0020] Figure 2 A schematic diagram of the unfolded structure of the door panel of the rapid culture box for mesenchymal stem cell culture provided by this utility model;

[0021] Figure 3 A schematic cross-sectional view of the main body of the rapid culture box for mesenchymal stem cell culture provided by this utility model;

[0022] Figure 4 A schematic diagram of the internal structure of the water tank in the rapid culture box for mesenchymal stem cell culture provided by this utility model;

[0023] Figure 5 This is a top cross-sectional view of the rapid culture box for mesenchymal stem cell culture provided by this utility model.

[0024] Legend: 1. Cultivation chamber; 2. Sealing groove; 3. Door panel; 4. Pull groove; 5. Mounting groove; 6. Fan; 7. Filter screen; 8. Heating rod; 9. Placement plate; 10. Ventilation hole; 11. Pure water placement area; 12. First heating controller; 13. Heating wire; 14. Second heating controller; 15. Temperature sensor; 16. Humidity sensor; 17. Connecting seat; 18. Spray nozzle; 19. Connecting pipe; 20. Water tank; 21. Water pump. Detailed Implementation

[0025] The technical solutions of the present utility model will be clearly and completely described below with reference to the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the protection scope of the present utility model.

[0026] To facilitate understanding of this utility model, a more comprehensive description of this utility model will be provided below with reference to relevant embodiments, and several embodiments of this utility model will be given. However, this utility model can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of this utility model more thorough and complete.

[0027] It should be noted that when an element is referred to as being "fixed to" another element, it can be directly on the other element or there may be an intervening element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or there may be an intervening element. The terms "vertical," "horizontal," "left," "right," and similar expressions used in this document are for illustrative purposes only.

[0028] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0029] Example

[0030] like Figure 1-5 As shown, this utility model provides a technical solution: a rapid culture box for mesenchymal stem cell culture. The culture box 1 is the core body of the entire device and is made of a material with good heat preservation and sealing properties. This box provides a relatively independent, closed and stable spatial environment for cell culture, effectively isolating the external environment from interference that may occur during the cell culture process.

[0031] The bottom of the cultivation chamber 1 is provided with an installation groove 5. The size and shape of the installation groove 5 are designed according to the specifications of the auxiliary heating component, providing a stable and suitable installation position for the auxiliary heating component, ensuring that it will not be displaced during operation, thereby ensuring the stable performance of the heating function.

[0032] The inner wall of the cultivation chamber 1 is cleverly designed with a pure water storage area 11. This area is specifically for storing pure water, which plays a crucial role in the entire temperature and humidity regulation process. The heating wire 13 installed in this area is a special metal wire with high resistance and high temperature resistance. When current passes through it, according to Joule's law (Q=I... 2 Rt, where Q represents heat, I represents current, R represents resistance, and t represents time), the heating wire 13 generates a large amount of heat. A first heating controller 12 is installed at the connection of the heating wire 13 and on the left side wall of the incubation chamber 1. This is a high-precision electronic control device that can accurately adjust the current through the heating wire 13 according to the preset temperature parameters and the data fed back by the temperature sensor 15.

[0033] When a higher temperature is required, the current is increased to generate more heat from the heating wire 13, which heats the water in the pure water placement area 11. The heated water is maintained within a certain temperature range, which in turn allows for uniform heating and maintenance of the water adhering to the inner wall. This method of regulating temperature by heating water makes the environment inside the chamber more mild and uniform, avoiding adverse effects on cell culture caused by excessively high or low local temperatures.

[0034] The top of the cultivation box 1 is equipped with a humidification component, which is an important part of maintaining suitable humidity inside the box. The humidification component includes a connecting seat 17, which is a hollow structure with several spray nozzles 18 evenly distributed at its bottom. The size and spacing of these spray nozzles 18 are carefully designed to ensure that the sprayed water mist is evenly distributed inside the box. The top of the connecting seat 17 is equipped with a connecting pipe 19, which connects the connecting seat 17 to the water storage tank 20.

[0035] The water storage tank 20 is fixedly installed on the back side of the culture chamber 1. It has sufficient capacity to store water for humidification. The water storage tank 20 is equipped with a water pump 21. The water pump 21 is a device that uses a motor to drive the impeller to rotate to achieve liquid transportation. When the water pump 21 is started, it draws water from the water storage tank 20 into the connecting pipe 19. Then the water is sprayed out from the spray nozzle 18 through the connecting seat 17 to form a fine water mist. This water mist works in conjunction with the auxiliary heating components in the chamber to make it evaporate quickly, thereby increasing the humidity in the chamber. This humidification method can quickly and effectively replenish the water lost in the chamber due to various factors (such as air circulation, temperature regulation, etc.), and maintain a stable humidity environment for cell culture.

[0036] Several temperature sensors 15 are installed inside the cultivation chamber 1 on the left side, and a humidity sensor 16 is installed on the right side. The temperature sensors 15 are made using physical principles such as thermal expansion and contraction and thermoelectric effect, and can convert temperature changes into electrical signals. These temperature sensors 15 are distributed in different positions, which can comprehensively and accurately monitor the temperature of various areas inside the chamber.

[0037] The humidity sensor 16 obtains humidity information by measuring the water vapor content in the air. Common principles include capacitive and resistive. When the humidity in the air changes, the capacitance or resistance value of the humidity sensor 16 will change accordingly, thereby converting the humidity information into an electrical signal. These sensors feed back the real-time temperature and humidity data to the control system (PLC control computer). The control system precisely regulates the heating wire 13, auxiliary heating components and humidification components according to the preset parameters and feedback data to ensure that the temperature and humidity inside the chamber are always kept within the range most suitable for mesenchymal stem cell culture.

[0038] The interior of the culture chamber 1 is also equipped with several placement plates 9. These placement plates 9 are made of materials that are non-toxic, harmless and have good stability for cell culture. The surface of the placement plates 9 is smooth and flat, which makes it easy to place cell culture equipment such as culture dishes and culture flasks. Several ventilation holes 10 are opened between the placement plates 9 and the bottom of the culture chamber 1. The design of the ventilation holes 10 has an important principle. According to the principle of aerodynamics, when there is a difference in temperature or humidity inside the chamber, the air will flow naturally.

[0039] Ventilation holes 10 provide a channel for air flow, allowing the air inside the chamber to circulate between different areas. This circulation helps to distribute heat and moisture evenly, making the temperature and humidity in all parts of the chamber more uniform and consistent. This creates a stable and balanced environment for cell culture and avoids the adverse effects on cell growth caused by local environmental differences.

[0040] The auxiliary heating component includes two fans 6. Each fan 6 is a device that generates airflow by rotating blades driven by a motor. During operation, the motor drives the blades to rotate at high speed. According to Bernoulli's principle, the rotation of the blades increases the airflow speed, thereby generating airflow. Below the fans 6 is a filter 7, which is a filtration device with fine mesh that can effectively block dust, impurities and other tiny particles in the air. When air is drawn in by the fans 6, the filter 7 filters out the impurities, preventing them from entering the chamber and contaminating the cell culture environment or affecting the normal operation of the heating component.

[0041] Several heating rods 8 are located above the fan 6. The heating rod 8 is a heating element that uses the principle of resistance heating. When current passes through the resistance wire inside the heating rod 8, the resistance wire heats up. Each of the heating rods 8 has a second heating controller 14 on its left side. The second heating controller 14 is similar to the first heating controller 12. It also precisely controls the current passing through the heating rod 8 based on preset parameters and data fed back by the temperature sensor 15. The heating rod 8 is electrically connected to the heating wire 13. When rapid heating or auxiliary heating of a specific area inside the chamber is required, the fan 6 rotates to promote airflow. This allows the heat generated by the heating rod 8 to spread rapidly inside the chamber with the airflow. Together with the heat generated by the heating wire 13, the heat inside the chamber is rapidly increased and the temperature distribution is made more uniform.

[0042] The front of the incubator 1 has a sealing groove 2, and a door panel 3 is located inside the sealing groove 2. The door panel 3 has a groove 4. The door panel 3 is inserted into the sealing groove 2 to achieve a seal. The door panel 3 is made of the same material as the incubator body and has good heat insulation and sealing performance. The design of the sealing groove 2 and the door panel 3 fits tightly. When the door panel 3 is inserted into the sealing groove 2, a continuous sealing interface is formed between the two. This sealing method utilizes the elasticity and friction of the material, which can effectively prevent heat loss from the incubator to the external environment, and at the same time prevent outside air from entering the incubator. This avoids the contamination of the cell culture environment by dust, microorganisms, etc. from the external environment, and ensures the stability and purity of the internal environment of the incubator.

[0043] In summary, the specific workflow is as follows: the equipment is started, the PLC control computer is initialized, and the operator sets the target temperature and humidity parameters on the control computer. These parameters are determined based on the optimal environmental conditions for mesenchymal stem cell culture.

[0044] After startup, the first heating controller 12 starts according to the set parameters, controlling the heating wire 13 to heat the water in the pure water placement area 11. The current passes through the heating wire 13, the heating wire 13 generates heat, and the heat is transferred to the pure water. The water temperature gradually rises, causing the temperature inside the chamber to rise. At the same time, the humidity also increases accordingly. During this process, the temperature sensor 15 and the humidity sensor 16 monitor the temperature and humidity data inside the chamber in real time and feed the data back to the PLC control computer.

[0045] When the temperature sensor 15 detects that the temperature inside the chamber has not yet reached the target temperature, the PLC control computer starts the auxiliary heating component. The fan 6 motor starts, and the blades rotate to generate airflow. Air is drawn in through the filter 7, which filters out dust and other impurities. At the same time, the second heating controller 14 controls the current to pass through the heating rod 8 based on the data fed back by the temperature sensor 15. The heating rod 8 heats up, and the airflow generated by the fan 6 carries the heat generated by the heating rod 8 and flows rapidly inside the chamber. This heat, combined with the heat generated by the heating wire 13, causes the temperature inside the chamber to rise rapidly and promotes a more uniform temperature distribution. During this process, the temperature sensor 15 continuously monitors temperature changes and feeds back the real-time data to the PLC control computer. When the temperature reaches the target temperature, the PLC control computer adjusts the power of the auxiliary heating component or stops heating according to the preset control logic to maintain a stable temperature.

[0046] Humidity sensor 16 simultaneously monitors the humidity inside the chamber. When the humidity falls below the target humidity, the PLC control computer activates the humidification component, and the water pump 21 in the water tank 20 starts. The motor drives the impeller to rotate, drawing water from the water tank 20. The water enters the connecting seat 17 through the connecting pipe 19, and then is sprayed out from the spray nozzle 18 at the bottom of the connecting seat 17, forming a fine water mist. This water mist evaporates rapidly inside the chamber, increasing the humidity. Humidity sensor 16 feeds back the humidity data to the PLC control computer in real time. When the humidity reaches the target humidity, the PLC control computer controls the water pump 21 to stop working, maintaining a stable humidity level.

[0047] Throughout the culture process, the cell culture apparatus placed on the placement plate 9 is cultured in a stable temperature and humidity environment. The placement plate 9 provides stable support for the culture apparatus, and the ventilation holes 10 ensure the circulation of air inside the chamber, so that the temperature and humidity can be kept uniform around each culture apparatus, creating ideal conditions for cell growth, proliferation and differentiation.

[0048] Throughout the process, door panel 3 remains closed and tightly inserted into the sealing groove 2, ensuring the airtightness of the incubator. This airtightness effectively prevents heat loss from the incubator and interference from external air, maintaining the stability of the internal environment and ensuring that cell culture is not affected by external factors.

[0049] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A rapid culture box for mesenchymal stem cell culture, comprising a culture box body (1), characterized in that: The bottom of the cultivation chamber (1) is provided with an installation groove (5), and an auxiliary heating component is installed inside the installation groove (5). A pure water placement area (11) is provided in the inner wall of the cultivation chamber (1). A heating wire (13) is provided in the pure water placement area (11). A first heating controller (12) is installed at the connection of the heating wire (13) and on the left side wall of the cultivation chamber (1). A humidification component is provided at the top of the cultivation chamber (1). Several temperature sensors (15) are installed inside the cultivation chamber (1) and on the left side. A humidity sensor (16) is installed inside the cultivation chamber (1) and on the right side.

2. The rapid culture box for mesenchymal stem cell culture according to claim 1, characterized in that: The incubation box (1) is also provided with several placement plates (9), and several ventilation holes (10) are opened between the several placement plates (9) and the bottom of the incubation box (1). The pure water placement area (11) is filled with water.

3. The rapid culture box for mesenchymal stem cell culture according to claim 2, characterized in that: The auxiliary heating assembly includes two fans (6), each with a filter (7) below it, and several heating rods (8) above it. Each of the heating rods (8) has a second heating controller (14) on its left side.

4. The rapid culture box for mesenchymal stem cell culture according to claim 3, characterized in that: The fan (6) and the filter (7) work together, and the heating rod (8) is electrically connected to the heating wire (13).

5. The rapid culture box for mesenchymal stem cell culture according to claim 4, characterized in that: The front of the cultivation box (1) is provided with a sealing groove (2), and a door panel (3) is provided inside the sealing groove (2). The door panel (3) is provided with a pull groove (4). The door panel (3) is inserted into the sealing groove (2) for use, thereby achieving a seal.

6. The rapid culture box for mesenchymal stem cell culture according to claim 5, characterized in that: The humidification assembly includes a connecting seat (17), the bottom of which is provided with a plurality of spray nozzles (18), and the top of which is provided with a connecting pipe (19).

7. The rapid culture box for mesenchymal stem cell culture according to claim 6, characterized in that: The humidification assembly also includes a water storage tank (20), which is fixedly installed on the back side of the cultivation box (1). The water storage tank (20) is equipped with a water pump (21) inside, and the water pump (21) is connected to the connecting pipe (19).

8. The rapid culture box for mesenchymal stem cell culture according to claim 7, characterized in that: The humidification component and the humidity sensor (16) work together, and the auxiliary heating component and the heating wire (13) work together with the temperature sensor (15). The humidification component, the auxiliary heating component, the humidity sensor (16), the temperature sensor (15) and the heating wire (13) are all connected to the PLC control computer.

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