Stem cell cryopreservation protection liquid filtering device

Abstract

The utility model belongs to the technical field of stem cell preservation, and particularly relates to stem cell cryopreservation protection liquid filtration. The device comprises a feeding device, a filtering device and a material receiving bottle, the feeding device is connected to the upper portion of the filtering device, and the material receiving device is connected to the lower portion of the filtering device; the feeding device comprises a feeding hopper and a discharging pipe, the feeding hopper is of a closed structure, a feeding port and a compressed gas inlet are formed in the feeding hopper, and the discharging pipe is arranged below the feeding hopper; the material receiving device comprises a liquid collecting bottle and a material receiving pipe, and the material receiving pipe is arranged above the liquid collecting bottle; the filtering device comprises a base, a microporous filter disc, an upper connecting pipe and a lower connecting pipe, the inner circumference of the base is designed to be a cavity, a base bottom plate is arranged at the bottom of the cavity, the microporous filter disc is mounted in the cavity above the base bottom plate, the shape of the microporous filter disc is consistent with that of the section of the cavity, the upper connecting pipe is detachably connected with the discharging pipe, and the lower connecting pipe is detachably connected with the material receiving pipe; the utility model provides a stem cell cryopreservation protection liquid filtering device which is convenient, efficient and easy to clean.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the stem cell preservation technical field especially relates to the freeze storage protection solution of stem cell filters. BACKGROUND

[0002] The most common stem cell preservation method at present is through the cryopreservation. This method utilizes the low temperature biology principle, cools the cell to extremely low temperature (usually -196 DEG C in liquid nitrogen), under this condition, the cell metabolism almost completely stops, so that the cell can be preserved for a long time without damaging its function, in the preservation process, in order to reduce the ice crystal formed in the freezing process to the damage of cell structure, usually will be added the freeze protection solution containing dimethyl sulfoxide (DMSO) composition, for example, the most common freeze protection solution formula is as follows: base medium 70%, dimethyl sulfoxide (DMSO) 20%, fetal bovine serum (FBS) 10%, even if in the preparation process as far as possible keep clean, there can be tiny particles or undissolved substances remaining in the solution, needs to remove these impurities before use, guarantees the purity of solution, the existing technology such as the announcement number for: CN220513881 U's utility model proposes a kind of cell freeze storage solution filter, is provided with mixing box, filter box, stirring part, flow guide part, filter cartridge and sealing plate and multiple structures, when stirring part stirs liquid in mixing box, then flow guide part extracts liquid to filter box, filters liquid by filter cartridge, the device structure is complex, needs flow guide part to extract liquid to filter cartridge and the design of etc. cause liquid flow path to be too long, reduce the filtration efficiency, filter cartridge and sealing plate and the components such as part are difficult to disassemble and clean, long-term use can accumulate dirt and contaminate stem cell freeze protection solution. UTILITY MODEL CONTENTS

[0003] Therefore, the utility model aims at providing a kind of stem cell freeze protection solution filter device, to solve the problems in prior art.

[0004] To achieve the above-mentioned purpose, the technical scheme adopted by the utility model is: a stem cell freeze protection solution filter device, characterized by comprising: a feeding device, a receiving device, a filtering device, the feeding device is connected above the filtering device, the receiving device is connected below the filtering device,

[0005] The feeding device comprises a feeding hopper and a discharge pipe. The feeding hopper is airtight, and the feeding hopper is provided with a feeding port and a compressed gas inlet. The discharge pipe is arranged below the feeding hopper.

[0006] The receiving device comprises a liquid collecting bottle and a receiving pipe. The receiving pipe is arranged above the liquid collecting bottle.

[0007] The filter device comprises a base, a microporous filter sheet, an upper connecting pipe and a lower connecting pipe, the inner periphery of the base is designed as a cavity, the bottom of the cavity is provided with a base bottom plate, the base bottom plate is provided with a leakage hole, the microporous filter sheet is installed in the cavity above the base bottom plate, the shape of the microporous filter sheet is consistent with the cross-sectional shape of the cavity, the upper connecting pipe is arranged above the base, the upper connecting pipe is detachably connected with the lower pipe, and the lower connecting pipe is arranged below the base and is detachably connected with the material receiving pipe.

[0008] Further, the outer wall of the upper connecting pipe is provided with an upper outer thread, the inner wall of the lower pipe is provided with an upper inner thread matched with the upper outer thread, and the upper connecting pipe is externally provided with an upper sealing gasket; the outer wall of the lower connecting pipe is provided with a lower outer thread, the inner wall of the material receiving pipe is provided with a lower inner thread matched with the lower outer thread, and the lower connecting pipe is externally provided with a lower sealing gasket.

[0009] Further, the filter device further comprises a filter plate, the filter plate is provided with a filter hole, and the filter plate is arranged above the microporous filter sheet.

[0010] Further, the upper hopper comprises a hopper body and a sealing cover, an annular boss extending upwards is arranged on the upper edge of the hopper body, an outer sealing ring is arranged on the outer periphery of the annular boss, the sealing cover is buckled on the hopper body, the inner wall of the sealing cover is matched with the annular boss, and an inner sealing ring is arranged on the inner wall surface of the sealing cover.

[0011] Further, the feeding port and the compressed gas inlet are arranged on the outer side wall of the sealing cover.

[0012] Further, the compressed gas inlet is arranged on the side opposite to the feeding port.

[0013] Further, the liquid collecting bottle is provided with a suction filtration interface.

[0014] Further, the liquid collecting bottle is provided with a metering scale.

[0015] The beneficial effects generated by the above technical scheme are as follows: 1. The flexible connection mode facilitates easy disassembly and cleaning or replacement of each part, effectively avoiding pollution caused by residues;

[0016] 2. The filter structure is arranged according to the natural flow direction of the stem cell cryopreservation protective solution, avoiding long pipelines and extraction operations, being simple and efficient, and improving work efficiency. BRIEF DESCRIPTION OF DRAWINGS

[0017] In order to more clearly illustrate the technical scheme in the embodiments of the present application, the drawings needed to be used in the following embodiment description will be briefly introduced. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can also be obtained according to these drawings without creative labor for those skilled in the art.

[0018] Figure 1 is a structural schematic diagram of the stem cell cryopreservation protective solution filtering device provided by the embodiments of the present application;

[0019] Figure 2 is a sectional structure schematic diagram of the filtering device provided by the embodiments of the present application;

[0020] Figure 3 is an exploded structure diagram of the base of the filtering device provided by the embodiments of the present application;

[0021] Figure 4 is a disassembled structure schematic diagram of the feeding hopper provided by the embodiments of the present application;

[0022] Explanation of the reference signs:

[0023] 1-feeding device, 11-feeding hopper, 111-hopper body, 112-annular boss, 112A-outer sealing ring, 113-sealing cover, 113A-inner sealing ring, 114-feeding port, 115-compressed gas inlet, 12-discharging pipe, 121-upper inner thread;

[0024] 2-filtering device, 21-base, 211-cavity, 212-base bottom plate, 212A-leakage hole, 213-micro-porous filter sheet, 214-filter plate, 214A-filter hole, 22-upper connecting pipe, 221-upper outer thread, 222-upper sealing washer, 23-lower connecting pipe, 231-lower outer thread, 232-lower sealing washer;

[0025] 3-receiving device, 31-liquid collecting bottle, 311-suction filtration interface, 312-measuring scale, 32-receiving pipe, 321-lower inner thread. DETAILED DESCRIPTION

[0026] In the following description, specific details are set forth in order to provide a thorough understanding of the embodiments of the present application for purposes of explanation and not limitation. It will be obvious to those skilled in the art that the present application can be practiced without some or all of these specific details. In other instances, well-known systems, structures, circuits, and materials have not been described in detail in order to avoid unnecessarily obscuring the description of the present application.

[0027] In order to illustrate the technical scheme of the utility model, the following is described by means of specific embodiments. The utility model embodiment provides a stem cell cryopreservation protective solution filtering device, which is combined with Figure 1 , Figure 2 As shown in the drawings, the stem cell cryopreservation protective solution filtering device comprises a feeding device 1, a receiving device 3 and a filtering device 2. The feeding device 1 is connected to the top of the filtering device 2, and the receiving device 3 is connected to the bottom of the filtering device 2. The feeding device 1 comprises a feeding hopper 11 and a discharging pipe 12. The feeding hopper 11 is of a closed structure, and a feeding port 114 and a compressed gas inlet 115 are arranged on the feeding hopper 11. The discharging pipe 12 is arranged below the feeding hopper 11. The receiving device 3 comprises a liquid collecting bottle 31 and a receiving pipe 32. The receiving pipe 32 is arranged above the liquid collecting bottle 31. The filtering device 2 comprises a base 21, a microporous filter 213, an upper connecting pipe 22 and a lower connecting pipe 23. The inner periphery of the base 21 is designed as a cavity 211. A base bottom plate 212 is arranged at the bottom of the cavity 211. The base bottom plate 212 is provided with a leakage hole 212A. The microporous filter 213 is installed in the cavity 211 above the base bottom plate 212. The shape of the microporous filter 213 is consistent with the cross-sectional shape of the cavity 211. The upper connecting pipe 22 is arranged above the base, and the upper connecting pipe 22 is detachably connected to the discharging pipe 12. The lower connecting pipe 23 is arranged below the base, and the lower connecting pipe 23 is detachably connected to the receiving pipe 32.

[0028] In use, the operator assembles the receiving device 3, the filtering device 2 and the feeding device 1 in turn from bottom to top. After stable installation, the stem cell cryopreservation protective solution is injected through the feeding port 114, and the compressed air is connected through the compressed gas inlet 115 to start the filtering process. After the operation is completed, the entire device can be conveniently disassembled for cleaning and maintenance according to needs. The utility model is installed according to the natural flow of liquid, the flow direction pipeline is simple and efficient, and the filtering efficiency is effectively improved. The entire device is convenient to install and disassemble, has no structural dead angle, and cleaning operation can be quickly completed after use.

[0029] As an embodiment, the utility model is combined with Figures 1-3The outer wall of the upper connecting pipe 22 is provided with an upper outer thread 221, the inner wall of the lower pipe 12 is provided with an upper inner thread 121 matched with the upper outer thread 221, and the outer part of the upper connecting pipe 22 is provided with an upper sealing washer 222; the outer wall of the lower connecting pipe 23 is provided with a lower outer thread 231, the inner wall of the lower pipe 32 is provided with a lower inner thread 321 matched with the lower outer thread 231, and the outer part of the lower connecting pipe 23 is provided with a lower sealing washer 232, so as to realize the installation and fastening of the material receiving device 3, the filtering device 2 and the material feeding device 1 through the threaded port, and the staff can rotate the threaded port of the material receiving pipe 32 and the lower connecting pipe 23 when operating, complete the installation of the material receiving device 3 and the filtering device 2, rotate the threaded port of the lower pipe 12 and the upper connecting pipe 22, complete the installation of the material feeding device 1 and the filtering device 2, and the whole installation process is convenient and efficient, and the structure is firm; meanwhile, the threaded port is provided with a sealing washer, so as to prevent rigid damage during installation and ensure the air tightness of the device.

[0030] At the same time of improving the filtering effect, the microporous filter is further protected, as an embodiment, in combination with Figures 1-3 As shown, the filtering device 2 is further provided with a filter pressing plate 214, the filter pressing plate 214 is provided with filter holes 214A, and the filter pressing plate 214 is above the microporous filter 213, so that the filter pressing plate 214 can reduce the impact of liquid on the microporous filter 213, and can also compress the microporous filter 213, protect the microporous filter 213, improve the filtering stability, and the filter holes 214A on the filter pressing plate 214 can play a coarse filtering effect, and further improve the filtering performance.

[0031] In order to further improve the material feeding device, as an embodiment, in combination with Figure 1 , Figure 4 As shown, the material feeding hopper 11 includes a hopper body 111 and a sealing cover 113; the upper edge of the hopper body 111 is provided with an annular boss 112 extending upward, the outer periphery of the annular boss 112 is provided with an outer sealing ring 112A, the sealing cover 113 is buckled on the hopper body 111, the inner wall of the sealing cover 113 is in close contact with the annular boss 112, and the inner wall surface of the sealing cover 113 is provided with an inner sealing ring 113A; the material feeding hopper 11 is designed with an openable and closable sealing cover structure, which can ensure the sealing performance and facilitate timely checking when the feeding is abnormal during the filtering process.

[0032] As an embodiment, in combination with Figure 1 , Figure 4 As shown, the feeding port 114 and the compressed gas inlet port 115 are arranged on the outer side wall of the sealing cover 113, which is more convenient for installation and disassembly, and when the whole device is disassembled, the sealing cover with the attached interface is placed aside first to prevent the influence of the attached interface.

[0033] As an embodiment, in combination with Figure 1 , Figure 4As shown, the compressed gas inlet 115 is arranged on the opposite side of the feed inlet 114, preventing the influence of the compressed gas on the feed.

[0034] As an embodiment, in combination with Figure 1 As shown, the liquid collecting bottle 31 is provided with a suction interface 311, which can be connected with a vacuum device to perform suction during the filtration operation, further improving the filtration efficiency.

[0035] As an embodiment, in combination with Figure 1 As shown, the liquid collecting bottle 31 is provided with a metering scale 312, facilitating the metering of the liquid during the filtration process.

[0036] The above-described embodiments are only used to illustrate the technical solutions of the present application, rather than limit them; although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that the technical solutions recorded in the foregoing embodiments can still be modified, or some technical features can be replaced equivalently; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application, and should be included in the protection scope of the present application.

Claims

1. A filtration device for stem cell cryopreservation solution, characterized in that, The utility model relates to a kind of filter device and its connecting pipe, including: Feeding device (1), receiving device (3), filter device (2), the feeding device (1) is connected above the filter device (2), the receiving device (3) is connected below the filter device (2), The feeding device (1) includes feeding hopper (11), discharging pipe (12), the feeding hopper (11) is closed structure, feeding inlet (114) and compressed gas inlet (115) are provided on the feeding hopper (11), the discharging pipe (12) is arranged below the feeding hopper (11); The receiving device (3) includes liquid collecting bottle (31), receiving pipe (32), the receiving pipe (32) is arranged above the liquid collecting bottle (31); The filter device (2) includes base (21), microporous filter sheet (213), upper connecting pipe (22), lower connecting pipe (23), the inner periphery of the base (21) is designed as cavity (211), the bottom of the cavity (211) is provided with base bottom plate (212), the base bottom plate (212) is provided with leakage hole (212A), the microporous filter sheet (213) is installed in the cavity (211) above the base bottom plate (212), the shape of the microporous filter sheet (213) is consistent with the cross-sectional shape of the cavity (211), the upper connecting pipe (22) is arranged above the base, the upper connecting pipe (22) is detachably connected with the discharging pipe (12), the lower connecting pipe (23) is arranged below the base, and the lower connecting pipe (23) is detachably connected with the receiving pipe (32).

2. The stem cell cryopreservation solution filtration device of claim 1, wherein, The outer wall of the upper connecting pipe (22) is provided with upper outer thread (221), the inner wall of the discharging pipe (12) is provided with upper inner thread (121) matched with the upper outer thread (221), and the outer part of the upper connecting pipe (22) is provided with upper sealing gasket (222); the outer wall of the lower connecting pipe (23) is provided with lower outer thread (231), the inner wall of the receiving pipe (32) is provided with lower inner thread (321) matched with the lower outer thread (231), and the outer part of the lower connecting pipe (23) is provided with lower sealing gasket (232).

3. The stem cell cryopreservation solution filtration device of claim 2, wherein, The filter device (2) further includes filter plate (214), the filter plate (214) is provided with filter hole (214A), and the filter plate (214) is arranged above the microporous filter sheet (213).

4. The stem cell cryopreservation solution filtration device of claim 2, wherein, The feeding hopper (11) includes hopper body (111) and sealing cover (113), the upper edge of the hopper body (111) is provided with annular boss (112) extending upward, the outer periphery of the annular boss (112) is provided with outer sealing ring (112A), the sealing cover (113) is buckled on the hopper body (111), the inner wall of the sealing cover (113) is fitted with the annular boss (112), and the inner wall surface of the sealing cover (113) is provided with inner sealing ring (113A).

5. The stem cell cryopreservation solution filtration device of claim 4, wherein, The feeding inlet (114) and the compressed gas inlet (115) are both arranged on the outer sidewall of the sealing cover (113).

6. The stem cell cryopreservation solution filtration device of claim 5, wherein, The compressed gas inlet (115) is arranged on the opposite side of the feeding inlet (114).

7. The stem cell cryopreservation solution filtration device of claim 6, wherein, The siphon bottle (31) is provided with a siphon interface (311).

8. The stem cell cryopreservation solution filtration device of claim 7, wherein, The siphon bottle (31) is provided with a metering scale (312).

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