Oligonucleotide ammonolysis box

Abstract

The utility model discloses an oligonucleotide ammonolysis box which comprises a box body, the box body comprises a containing cavity and an annular interlayer, and the interlayer divides the containing cavity into an upper cavity and a lower cavity which are communicated together; the box cover is detachably mounted at the top of the box body; the elastic bearing structures are arranged at the four corners of the bottom wall of the lower cavity, each elastic bearing structure comprises a reset spring arranged on the inner wall of the lower cavity, and a supporting plate is fixed to the top face of each reset spring; and the pair of extrusion rods are fixed on the bottom surface of the box cover and are symmetrically distributed, and the extrusion rods are positioned at the edge of the placed composite plate. According to the utility model, the box body is placed in a fuming cupboard, ammonia water is added into the lower cavity, a synthesis plate with a synthesis column is placed between the interlayers of the box body, the box cover is covered, and the box body is transferred into a water bath kettle or a drying oven to be heated for ammonolysis.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of nucleotide ammonolysis, specifically to an oligonucleotide ammonolysis box. BACKGROUND

[0002] Oligonucleotides are currently mainly synthesized by solid-phase phosphoramidite synthesis method, and the synthesized product is fixed on a solid support. After synthesis, we need to transfer it to a liquid system. Therefore, we need to use related reagents to break the linkage between it and the support. This process is called ammonolysis. In addition to separating oligonucleotides from solid supports, ammonolysis also has two other functions. One is deprotection. The amino groups on the bases of oligonucleotides are protected. Ammonolysis can remove the corresponding protecting groups to expose the amino groups. The other is that the ammonolysis process can remove the cyanoethyl group at position 3.

[0003] The existing oligonucleotide ammonolysis box can make the ammonolysis reaction occur in the ammonolysis box, and then heat it with an external heat source to form the environment required for the ammonolysis reaction in the cavity. This solves the problem of using an ammonia tank to deliver ammonia for ammonolysis, improves the safety of the operation, and is beneficial to batch production. For example, Chinese patent CN 215757106 U discloses an oligonucleotide ammonolysis box. During use, the user needs to wear heat-insulating gloves to take out the synthesis plate from the gap structure of the ammonolysis box. Although the ammonolysis box is designed with a flared structure, the process of taking out the synthesis plate is still not very convenient due to the thickness and weight of the heat-insulating gloves. SUMMARY

[0004] To overcome the shortcomings of the prior art, the utility model provides an oligonucleotide ammonolysis box. After opening the box cover, the synthesis plate can be automatically lifted, making it easy to take out the synthesis plate from the box body and improving the convenience of use.

[0005] The utility model provides an oligonucleotide ammonolysis box, which comprises:

[0006] A box body comprising a containing cavity and a ring-shaped partition, the partition dividing the containing cavity into an upper cavity and a lower cavity that are connected together;

[0007] A box cover detachably mounted on the top of the box body;

[0008] An elastic bearing structure arranged at the four corners of the bottom wall of the lower cavity, the elastic bearing structure comprising a return spring arranged on the inner wall of the lower cavity, and a support plate fixed to the top surface of the return spring;

[0009] A pair of extrusion rods fixed to the bottom surface of the box cover and symmetrically distributed, the position of the extrusion rods being located at the edge of the placed synthesis plate.

[0010] First, the ammonia is poured into the lower cavity, the synthesis plate with synthesis column is placed in the partition, then the upper opening of the upper cavity in the box is closed by the cover, the box is placed in the oven or water bath, the ammonia is decomposed into ammonia gas, the oligonucleotide on the synthesis plate can be realized by ammonia, and the reset spring of the elastic bearing structure lifts the synthesis plate, so that the synthesis plate is taken out from the partition.

[0011] As a further technical solution, the elastic bearing structure further comprises a T-shaped seat fixed on the inner wall of the lower cavity, the T-shaped seat has a movable rod that can move up and down, the support plate is fixed on the top surface of the movable rod, and the reset spring is sleeved on the outer periphery of the movable rod and located between the T-shaped seat and the support plate. The support plate can be pressed, and the tension generated after the reset spring is pressed can reset the support plate. The setting of the movable rod makes the movement of the support plate more stable.

[0012] As a further technical solution, the partition comprises an annular lower partition plate fixed on the inner wall of the box and an annular upper partition plate located above the annular lower partition plate, the radial width of the annular lower partition plate is greater than the radial width of the annular lower partition plate, and the four corners of the annular upper partition plate are provided with flares. The synthesis plate is placed on the top surface of the annular lower partition plate, the annular upper partition plate with smaller radial width can limit the synthesis plate, and the flares at the four corners of the annular upper partition plate facilitate the removal of the synthesis plate from the partition.

[0013] As a further technical solution, the box and the box cover are provided with connecting structures, the connecting structures comprise positioning columns fixed on the top surface of the box, the bottom surface of the box cover is provided with corresponding positioning grooves, and the box cover and the box are connected through a connecting rod pull buckle. When installing the box cover, the installation direction of the box cover is quickly positioned through the positioning columns and the positioning grooves, and then the box cover is locked on the box through the connecting rod pull buckle, so that the box and the box cover can be quickly installed and disassembled, and the ammonia decomposition reaction can be easily carried out.

[0014] As a further technical solution, the top surface of the box is fixed with a sealing ring, and the sealing ring is arranged between the box and the box cover. The sealing ring can be used to ensure the sealing performance of the box when the opening is closed. When the connecting rod pull buckle is locked, a downward force is applied to the box cover, so that the box cover and the box are tightly fitted, the sealing ring on the box is pressed, and the sealing effect is improved.

[0015] As a further technical solution, the top surface of the annular lower partition plate is fixed with a sealing ring. When the box cover is installed, the synthesis plate also has a certain downward movement space.

[0016] As a further technical scheme, the box cover is further provided with a heat insulation structure, the heat insulation structure comprises a rectangular fixed ring fixed on the top surface of the box cover, and a rectangular connecting ring is detachably installed on the rectangular fixed ring, and an annular heat insulation cover is fixed around the four sides of the box body and the box cover.

[0017] As a further technical scheme, the rectangular fixed ring and the rectangular connecting ring are connected through a pair of magnetically opposite magnets. One magnet is fixed on the rectangular fixed ring, and the other magnet is fixed on the rectangular connecting ring, so that the annular heat insulation cover can be conveniently installed and detached.

[0018] As a further technical scheme, the top surface of the box cover is further provided with a pressure indicator and a one-way valve. If the pressure in the box body exceeds the standard value, the box body can be vented by opening the one-way valve, thereby further improving the safety performance.

[0019] Compared with the prior art, the box body is placed in the fume hood, ammonia is added into the lower cavity, the synthesis board with the synthesis column is placed between the partitions of the box body, the box cover is covered, the box body is transported into the water bath kettle or the oven for heating for ammonolysis, after the ammonolysis is completed, the heatproof gloves are worn to take out the ammonolysis box, the ammonolysis box is transported into the fume hood, the box cover is opened, the synthesis board in the ammonolysis box is taken out and placed in the oven for drying for minutes, and after the synthesis film is completely dried, the synthesis film is placed in the fume hood for cooling, and the entire ammonolysis process is completed.

[0020] When the synthesis board is placed in the box body, the synthesis board is supported by the support plate of the elastic bearing structure, and the synthesis board is limited by the annular upper partition plate, at this time, the height of the synthesis board is higher than the height of the annular lower partition plate, the box cover is covered, the box cover extrudes the synthesis board, and the bottom surface of the synthesis board is attached to the top surface of the annular lower partition plate, at this time, the reset spring of the elastic bearing structure is in a compressed state, and when the box cover is opened again, the reset spring lifts the synthesis board, so that the synthesis board is conveniently taken out from the partition.

[0021] Moreover, when the box cover is installed, the installation direction of the box cover is quickly positioned through the positioning column and the positioning groove, and then the box cover is locked on the box body through the connecting rod pull buckle, so that the box body and the box cover can be quickly installed and detached, the ammonolysis reaction is facilitated, and since the connecting rod pull buckle can exert a downward force on the box cover during installation, the box body and the box cover are attached more closely, the sealing ring on the box body is extruded, and the sealing effect can be improved.

[0022] Meanwhile, after the ammonolysis box is taken out from the water bath kettle, the annular heat insulation cover can be installed, so that the ammonolysis box can be isolated, and the possibility of being scalded during transportation is reduced, the annular heat insulation cover is taken off after reaching the fume hood, and ventilation is continued. BRIEF DESCRIPTION OF DRAWINGS

[0023] In order to more clearly illustrate the technical solutions of the embodiments of the present application or the prior art, the drawings used in the embodiments or prior art description will be briefly introduced. Obviously, the drawings in the following description are some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor.

[0024] Figure 1 A structure schematic diagram of an oligonucleotide ammonolysis box provided by the present application.

[0025] Figure 2 A top view of a box body structure in an oligonucleotide ammonolysis box provided by the present application.

[0026] Figure 3 A schematic diagram of an elastic bearing structure in an oligonucleotide ammonolysis box provided by the present application.

[0027] Figure 4 A top view of a box cover structure of an oligonucleotide ammonolysis box provided by the present application.

[0028] Figure 5 A bottom view of a box cover structure of an oligonucleotide ammonolysis box provided by the present application.

[0029] Figure 6 A connection structure schematic diagram of a box cover and a heat insulation structure of an oligonucleotide ammonolysis box provided by the present application.

[0030] In the figure: 1, box body; 101, containing cavity; 1011, upper cavity; 1012, lower cavity; 102, partition layer; 1021, annular lower partition plate; 1022, annular upper partition plate; 1023, flared portion; 2, box cover; 3, elastic bearing structure; 301, T-shaped seat; 302, movable rod; 303, support plate; 304, return spring; 4, extrusion rod; 5, connection structure; 501, positioning column; 502, positioning groove; 503, connecting rod pull buckle; 6, sealing ring; 7, sealing ring; 8, heat insulation structure; 801, rectangular fixed ring; 802, rectangular connecting ring; 803, annular heat insulation cover; 804, magnet; 9, pressure indicator; 10, one-way valve. DETAILED DESCRIPTION

[0031] In the description, claims and drawings of the present utility model, the terms "comprising", "having" and any variations thereof are intended to cover non-exclusive inclusion. For example, a process, method, system, product or device that includes a series of steps or units does not necessarily have to be limited to those steps or units clearly listed, but may include other steps or units not clearly listed or inherent to these processes, methods, products or devices.

[0032] To make the objectives, technical solutions and advantages of the embodiments of the present utility model clearer, the technical solutions in the embodiments of the present utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the present utility model. Obviously, the described embodiments are some, but not all, of the embodiments of the present utility model. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts shall fall within the protection scope of the present utility model. In addition, the technical features in each embodiment or individual embodiment provided by the present utility model can be arbitrarily combined with each other to form a new technical solution. This combination is not restricted by the order of steps and / or the mode of structural composition, but must be based on the ability of those of ordinary skill in the art to implement. When the combination of technical solutions results in contradictions or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the protection scope required by the present utility model.

[0033] Please refer to Figures 1-6 , an oligonucleotide ammonolysis box is provided in an embodiment of the present utility model, which includes a box body 1. The box body 1 includes a receiving cavity 101 and a circular partition layer 102. The partition layer 102 divides the receiving cavity 101 into an upper cavity 1011 and a lower cavity 1012 that are connected together. There is an opening at the top of the upper cavity 1011, and the size of the opening is larger than the size of the synthesis plate. A box cover 2 is detachably installed on the top of the box body 1, and the box cover 2 can close the opening at the top of the receiving cavity 101 on the box body 1.

[0034] First, pour ammonia water into the lower cavity 1012, place the synthesis plate with the synthesis column (自带 by the DNA synthesizer) in the partition layer 102, then close the upper opening of the upper cavity 1011 in the box body 1 through the cover body 2, and place the box body 1 in an oven or a water bath for water bath heating. The ammonia water decomposes into ammonia gas, and thus ammonolysis of the oligonucleotide on the synthesis plate can be achieved.

[0035] Such as Figure 2As shown, the partition layer 102 is a stepped structure, the partition layer 102 includes an annular lower partition plate 1021 fixed on the inner wall of the box body 1, and an annular upper partition plate 1022 is also fixed on the inner wall of the box body 1 above the annular lower partition plate 1021, the radial width of the annular lower partition plate 1021 is greater than the radial width of the annular upper partition plate 1022, the synthetic plate is placed on the top surface of the annular lower partition plate 1021, and the annular upper partition plate 1022 with a smaller radial width can limit the synthetic plate, and the four corners of the annular upper partition plate 1022 are provided with flared portions 1023, so that the synthetic plate can be taken out from the partition layer 102.

[0036] In order to improve the convenience of taking out the synthetic plate from the partition layer 102, the four corners of the bottom wall of the lower cavity 1012 are provided with elastic bearing structures 3, which are elastically compressible structures, when the synthetic plate is placed in the box body 1, the synthetic plate is supported by the elastic bearing structures 3, and the annular upper partition plate 1022 limits the synthetic plate, at this time, the height of the synthetic plate is higher than the height of the annular lower partition plate 1021, the box cover 2 is closed, the box cover 2 extrudes the synthetic plate, so that the bottom surface of the synthetic plate is attached to the top surface of the annular lower partition plate 1021, at this time, the elastic bearing structures 3 are in a compressed state, when the cover is opened again, the elastic bearing structures 3 lift the synthetic plate, so that the synthetic plate can be taken out from the partition layer 102.

[0037] As a specific structure of an elastic bearing structure 3, as shown in the figure, Figure 3 The elastic bearing structure 3 includes a T-shaped seat 301 fixed on the inner wall of the lower cavity 1012, a pair of movable rods 302 that can move up and down on the T-shaped seat 301, a support plate 303 fixed on the top surfaces of the two movable rods 302, a reset spring 304 sleeved on the movable rods 302 between the T-shaped seat 301 and the support plate 303, the support plate 303 can be extruded to compress the reset spring 304, and the tension generated by the reset spring 304 after being extruded can also reset the support plate 303, and the arrangement of the movable rods 302 makes the movement of the support plate 303 more stable.

[0038] In addition, the bottom surface of the box cover 2 is also fixed with a pair of extrusion rods 4 symmetrically distributed, when the box cover 2 is closed on the box body 1, the extrusion rods 4 can extrude the synthetic plate, and the position of the extrusion rods 4 is located at the edge of the placed synthetic plate, which will not affect the placement of the synthetic column on the synthetic plate, by arranging the extrusion rods 4, when the box cover 2 is closed, the extrusion rods 4 can extrude the synthetic plate, so that the bottom surface of the synthetic plate is attached to the annular lower partition plate 1021, the bottom surface of the box cover 2 is attached to the top surface of the box body 1, at the same time, the arrangement of the extrusion rods 4 makes the top end of the synthetic column have a certain distance from the bottom surface of the box cover 2, which will not affect the ammonia decomposition.

[0039] As for how to lock the box cover 2 on the top surface of the box body 1, the box body 1 and the box cover 2 are provided with a connecting structure 5, as shown in the figure, Figure 2、 Figure 5 As shown in the drawings, the connecting structure 5 includes positioning columns 501 fixed to the top surface of the box body 1 at four corners, and the bottom surface of the box cover 2 is provided with four corresponding positioning grooves 502, and the box cover 2 is connected with the box body 1 through connecting rod buckles 503, and the connecting rod buckles 503 are provided around the box body 1 in four groups. When installing the box cover 2, first, the installation direction of the box cover 2 is quickly positioned through the positioning columns 501 and the positioning grooves 502, and then the box cover 2 is locked on the box body 1 through the connecting rod buckles 503, so that the box body 1 and the box cover 2 can be quickly installed and disassembled, and the ammonia decomposition reaction is facilitated.

[0040] In addition, in order to ensure the sealing during ammonia decomposition, the top surface of the box body 1 is fixed with a sealing ring 6, which is arranged between the box body 1 and the box cover 2, and can be used to ensure the sealing when the opening of the box body 1 is closed. When the connecting rod buckles 503 lock the box cover 2 and the box body 1, a downward force is applied to the box cover 2, so that the box body 1 and the box cover 2 are fitted more tightly, and the sealing ring 6 on the box body 1 is extruded, thereby improving the sealing effect. In addition, the top surface of the annular lower partition plate 1021 is fixed with a sealing ring 7, and when the box cover 2 is installed, the synthetic plate also has a certain downward movement space.

[0041] During use, the ammonia decomposition box needs to be placed in a water bath or an oven for heating and ammonia decomposition. After the ammonia decomposition is completed, the high-temperature ammonia decomposition box needs to be transported to a fume hood. The box cover 2 has a handle, which facilitates the transportation of the staff. If the transportation distance is long and the temperature of the ammonia decomposition box is high, it is easy to cause the staff to be scalded. A heat insulation structure 8 can also be arranged on the box cover 2 of the ammonia decomposition box to enhance its safety performance.

[0042] As a specific structure of the heat insulation structure 8, as shown in the drawings, Figure 4 、 Figure 6 The heat insulation structure 8 includes a rectangular fixed ring 801 fixed to the top surface of the box cover 2, and a rectangular connecting ring 802 detachably mounted on the rectangular fixed ring 801. The outer periphery of the rectangular connecting ring 802 is fixed with an annular heat insulation cover 803 which wraps around the four corners of the box body 1 and the box cover 2. After the ammonia decomposition box is taken out of the water bath, the annular heat insulation cover 803 is installed, so that the ammonia decomposition box can be isolated, reducing the possibility of being scalded during transportation. After reaching the fume hood, the annular heat insulation cover 803 is removed, and the ventilation is continued.

[0043] The rectangular fixed ring 801 and the rectangular connecting ring 802 are connected through a pair of magnets 804 with opposite magnetic properties. One of the two magnets 804 is fixed to the rectangular fixed ring 801, and the other magnet 804 is fixed to the rectangular connecting ring 802, so that the annular heat insulation cover 803 can be conveniently installed and disassembled.

[0044] As shown in the drawings, Figure 1 、 Figure 4As shown, the box cover 2 is further provided with a pressure indicator 9 and a one-way valve 10, if the pressure in the box body 1 exceeds the standard value, the box body 1 can be deflated by opening the one-way valve 10, further improving the safety performance.

[0045] In summary, the ammonia decomposition process of the ammonia decomposition box of the embodiment is as follows:

[0046] Place the box body 1 in the fume hood, then add ammonia water into the lower cavity 1012, wet the general synthesis column with 80% (v / v) acetonitrile (water: acetonitrile = 2:8), and then place the synthesis plate with the synthesis column between the layers 102 of the box body 1, cover the box cover 2, and then transfer the box body 1 to a 95% water bath or oven for heating for ammonia decomposition, and count 90 minutes. After the ammonia decomposition is completed, put on heat-resistant gloves to take out the ammonia decomposition box, transfer it to the fume hood, open the box cover, take out the synthesis plate inside the ammonia decomposition box, and dry it in the oven for 5 minutes. After the synthesis film is completely dried, cool it in the fume hood.

[0047] During use, when the synthesis plate is placed in the box body 1, the support plate 303 of the elastic bearing structure 3 supports the synthesis plate, and the annular upper partition plate 1022 limits the synthesis plate. At this time, the height of the synthesis plate is higher than the height of the annular lower partition plate 1021. Cover the box cover 2, and the pair of extrusion rods 4 of the box cover 2 extrude the synthesis plate, so that the bottom surface of the synthesis plate is attached to the top surface of the annular lower partition plate 1021. At this time, the return spring 304 is in a compressed state. After the ammonia decomposition is completed, the box cover 2 is removed, and the synthesis plate is popped up under the tension of the return spring 304, which facilitates the removal of the synthesis plate.

[0048] When installing the box cover 2, first quickly position the installation direction of the box cover 2 through the positioning column 501 and the positioning groove 502, and then lock the box cover 2 on the box body 1 through the connecting rod pull buckle 503, so that the box body 1 and the box cover 2 can be quickly installed and disassembled, facilitating the ammonia decomposition reaction.

[0049] After the ammonia decomposition box is taken out of the water bath, the annular heat shield 803 can be installed to isolate the ammonia decomposition box and reduce the possibility of being scalded during transportation. After reaching the fume hood, the annular heat shield 803 is removed and the ventilation is continued.

[0050] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application, and are not limited thereto. Although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the foregoing embodiments, or make equivalent substitutions for part or all of the technical features. Such modifications or substitutions do not change the essence of the corresponding technical solutions.

Claims

1. An oligonucleotide aminolysis cassette, characterized in that, The utility model relates to a synthetic board storage box, including: Box body (1), the box body (1) includes accommodating cavity (101) and annular partition (102), the partition (102) divides accommodating cavity (101) into upper cavity (1011) and lower cavity (1012) that communicate together, The detachable installation of box cover (2) is located at the top of the box body (1), The elastic bearing structure (3) is arranged at the four corners of the bottom wall of the lower cavity (1012), the elastic bearing structure (3) includes reset spring (304) arranged on the inner wall of the lower cavity (1012), the top surface of the reset spring (304) is fixed with support plate (303), A pair of extrusion rods (4) are fixed to the bottom surface of the box cover (2) and are symmetrically distributed, the position of the extrusion rod (4) is located at the edge of the placed synthetic board.

2. The oligonucleotide aminolysis cassette of claim 1, wherein, The elastic bearing structure (3) further includes a T-shaped seat (301) fixed to the inner wall of the lower cavity (1012), the T-shaped seat (301) has a movable rod (302) that can move up and down, the support plate (303) is fixed to the top surface of the movable rod (302), and the reset spring (304) is sleeved on the outer periphery of the movable rod (302) and located between the T-shaped seat (301) and the support plate (303).

3. The oligonucleotide aminolysis cassette of claim 1, wherein, The partition (102) includes an annular lower partition plate (1021) fixed to the inner wall of the box body (1) and an annular upper partition plate (1022) located above the annular lower partition plate (1021), the radial width of the annular lower partition plate (1021) is greater than the radial width of the annular lower partition plate (1021), and the four corners of the annular upper partition plate (1022) are provided with flares (1023).

4. The oligonucleotide aminolysis cassette of claim 3, wherein, The box body (1) and the box cover (2) are provided with a connecting structure (5), the connecting structure (5) includes a positioning column (501) fixed to the top surface of the box body (1), the bottom surface of the box cover (2) is provided with a corresponding positioning groove (502), and the box cover (2) and the box body (1) are connected through a connecting rod pull buckle (503).

5. The oligonucleotide aminolysis cassette of claim 4, wherein, The top surface of the box body (1) is fixed with a sealing ring (6), and the sealing ring (6) is arranged between the box body (1) and the box cover (2).

6. The oligonucleotide aminolysis cassette of claim 5, wherein, The top surface of the annular lower partition plate (1021) is fixed with a sealing ring (7).

7. The oligonucleotide aminolysis cassette of claim 1, wherein, The box cover (2) is further provided with a heat insulation structure (8), the heat insulation structure (8) includes a rectangular fixed ring (801) fixed to the top surface of the box cover (2), a rectangular connecting ring (802) is detachably mounted on the rectangular fixed ring (801), and an annular heat insulation cover (803) wrapping around the four corners of the box body (1) and the box cover (2) is fixed to the outer periphery of the rectangular connecting ring (802).

8. The oligonucleotide aminolysis cassette of claim 7, wherein, The rectangular fixed ring (801) and the rectangular connecting ring (802) are connected through a pair of magnetically opposite magnets (804).

9. The oligonucleotide aminolysis cassette of claim 1, wherein, The top surface of the box cover (2) is further provided with a pressure indicator (9) and a one-way valve (10).

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