Liquid-moving immunohistochemical device for liquid-based sample flaking

Abstract

The utility model provides a pipettable immunohistochemical device for preparing a liquid-based sample, which comprises a wet box placing box and liquid storage boxes which are independently arranged in a matrix shape in the wet box placing box, a gap is reserved between every two adjacent liquid storage boxes in each row to form a liquid discharge groove, and a corresponding filter screen is arranged on each liquid storage box; compared with a traditional immunohistochemical wet box, the liquid storage box and the filter screen which are independently arranged can be conveniently and freely combined, limitation caused by using a hydrophobic pen is eliminated, the liquid storage box and the filter screen which are adopted in the immunohistochemical wet box are both in a detachable mode, a label is arranged on the surface of the filter screen, a user can adjust according to area division, and therefore the immunohistochemical wet box is convenient to use. And a user can conveniently record the experiment.

Description

TECHNICAL FIELD

[0001] The utility model relates to biological experiment technical field, concretely is a kind of liquid-based sample preparation piece's movable immunohistochemical device. BACKGROUND

[0002] Immunohistochemistry is a method that uses the principle of antigen-antibody reaction to determine the antigen in tissue cells by making the color developing agent of labeled antibody develop color through chemical reaction.

[0003] The existing patch dyeing requires experimenters to select different specifications of immunostaining jars or immunohistochemical wet boxes according to the number of samples. When patch dyeing, experimenters select different specifications of immunostaining jars or immunohistochemical wet boxes according to the number of samples. When using commercial staining jars, due to the angle of placing the glass slide, after repeated elution, the tissue section may fall off from the glass slide, causing the loss of the sample. Most antibodies are quite expensive, so more wet boxes are chosen.

[0004] The current commercial immunohistochemical wet box is composed of a cover and a base. There are two water grooves in the base. Before using the wet box, a proper amount of deionized water needs to be added according to experience to ensure the environmental humidity during antibody incubation and avoid antibody evaporation. When using the wet box for antibody incubation and elution, a hydrophobic ring needs to be drawn around the patch with the help of an immunohistochemical pen, and the eluent is added in the hydrophobic ring for operation. Therefore, it cannot be shaken arbitrarily, and a shaking table cannot be used to accelerate the binding of the antibody and the antigen in the patch tissue. Patch dyeing often appears uneven dyeing, and the dyeing effect is quite different from that of bleaching. Moreover, the patch dyeing method is only suitable for thin (5-30 μm) section dyeing work. Furthermore, the existing immunohistochemical wet box needs to be repeatedly used with a pipette for manual operation for each antibody and reagent elution and incubation, and the operation needs to be very careful to avoid damaging the trace of the hydrophobic pen. Once the trace of the hydrophobic pen is damaged, it needs to be re-painted, which is time-consuming and laborious. Moreover, the hydrophobic pen has a volatile and pungent odor, and needs to be operated in a fume hood.

[0005] Therefore, the existing patent No. CN218973959U, a set of immunostaining tool kit for animal tissues and organs, adopts the technical scheme including an immunohistochemical wet box, which is also provided with a wet box filter sleeve and a wet box body, combines the technical advantages of the staining jar and the existing wet box, effectively protects the volatilization of sterile water in the wet box and maintains the environmental humidity in the box, changes the traditional patch dyeing method, and gets rid of the restriction of using the hydrophobic pen. Not only can it be combined with a wireless vibrator for use, greatly improving the dyeing effect, efficiency and convenience, but also can protect the experimenters from the harm of the volatile reagent of the hydrophobic pen to the body.

[0006] But the above technical solutions exist the following defects: Because the wet box body, the wet box filter sleeve plate, the wet box cover in the contrast file are integrally formed, when carrying out liquid exchange elution, the wet box filter sleeve plate needs to be taken down for a long time, the operation inconvenience is increased, and the risk of drying of the tissue cell sheet is increased, the staining effect is affected, and the position is most easily confused during overall replacement. Practical new type content

[0007] In view of the above-mentioned deficiencies in the prior art, the purpose of the present application is to provide a liquid-based sample preparation liquid transfer immunohistochemical device.

[0008] In order to achieve the above-mentioned purpose, the present application provides the following technical scheme: a liquid-based sample preparation liquid transfer immunohistochemical device, comprising: a wet box placing box, a plurality of liquid storage boxes arranged in a matrix in the wet box placing box, a gap is formed between each row of adjacent liquid storage boxes to form a liquid discharge groove, and a corresponding filter screen is arranged on each liquid storage box.

[0009] Further, the filter screen is provided with an anti-skid strip on the side facing the liquid storage box.

[0010] As a preferred embodiment of the present application, a hand holding part is arranged on the end face of the filter screen, a clamping point is arranged on the side of the hand holding part facing the liquid storage box, and a clamping hole matched with the clamping point is arranged on the liquid storage box.

[0011] Further, a liquid leakage hole is arranged on the liquid storage box, the liquid leakage hole is arranged towards the liquid discharge groove, and the liquid discharge groove extends through the wet box placing box in any radial direction.

[0012] Further, 1 / 3-2 / 3 of the height h of the liquid storage box is equal to the wall height d of the liquid storage box.

[0013] As a preferred embodiment of the present application, a box cover is arranged on the wet box placing box, clamping strips are arranged at any two ends of the box cover, clamping grooves matched with the clamping strips are arranged on both sides of the wet box placing box, and a gap is formed between the box cover and the liquid storage box.

[0014] Further, a pressing type buckle is arranged between the wet box placing box and the box cover, and a handle is arranged on the box cover.

[0015] Compared with the prior art, the present application has the following advantages:

[0016] Compared with the traditional immunohistochemical wet box, the present application can be freely combined with the independently arranged liquid storage box and the filter screen, and the restriction of using a hydrophobic pen is eliminated. In addition, the liquid storage box and the filter screen in the present application are detachable, a label is arranged on the surface of the filter screen, and the user can adjust according to the area division, which is convenient for the user to record the experiment. Attached Figure Description

[0017] Other features, objects, and advantages of this invention will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings:

[0018] Figure 1 This is a schematic diagram of the main structure of this utility model;

[0019] Figure 2 This is a schematic diagram of the liquid storage box and filter screen in this utility model;

[0020] Figure 3 This is a schematic diagram showing the installation positions of the locking protrusion and the locking slot in this utility model;

[0021] Figure 4 This is a schematic diagram showing the installation position of the box cover in this utility model.

[0022] In the diagram: 1. Wet box placement box; 11. Drainage trough; 12. Card slot; 2. Liquid storage box; 21. Locking opening; 22. Leakage outlet; 3. Filter screen; 31. Handle; 32. Anti-slip strip; 33. Locking protrusion; 4. Box lid; 41. Locking strip; 5. Press-type buckle; 6. Handle. Detailed Implementation

[0023] To make the technical means, creative features, objectives and effects of this utility model easier to understand, the present utility model will be further described below in conjunction with specific embodiments.

[0024] This application provides a pipette immunohistochemistry device for liquid-based sample preparation, comprising: a humidified chamber placement box 1, and independently arranged liquid storage boxes 2 in a matrix within the humidified chamber placement box 1. The liquid storage boxes 2 are limited by the walls of the humidified chamber placement box 1 or the walls of the drainage channels 11. Drainage channels 11 are provided within the humidified chamber placement box 1 and between adjacent liquid storage boxes 2 in each row to limit the position of the liquid storage boxes 2. Each liquid storage box 2 is provided with a corresponding filter 3. In actual operation, this application has multiple humidified chambers to meet the needs of continuous tissue and organ slide placement for one-time processing. The filter 3 greatly improves the efficiency of the experiment, reduces irreversible mechanical damage caused by repeated selection of tissue slides, and ensures the consistency of experimental parameters between each slide. It changes the traditional slide staining method and eliminates the limitations of using hydrophobic pens. Furthermore, the liquid storage boxes 2 and filter 3 used in this application are detachable. Labels are provided on the surface of the filter 3, allowing users to adjust them according to the area division, which is convenient for users to record experiments.

[0025] In actual operation, the liquid storage box 2 can be provided as an outer square and inner circle structure, so that the liquid storage box 2 can be individually taken out from the wet box placing box 1 for individual use. The application mainly shows the shape of outer square and inner square, but in actual operation, the liquid storage box 2 can also be provided as an arc shape structure specially used for fixing in the wet box placing box 1. It can also be provided as a polygon or circular structure with a flat bottom.

[0026] In order to ensure that the filter screen 3 is relatively fixed in the liquid storage box 2, the filter screen 3 is provided with an anti-skid strip 32 on one side of the filter screen 3 (i.e. on the outer surface of the filter screen 3), and the filter screen 3 is provided as a U-shaped structure for receiving the slice in the filter screen 3. The filter screen 3 is selected to have a micron level aperture of 100 μm.

[0027] In order to facilitate the taking of the filter screen 3, a hand holding part 31 is provided on the end surface of the filter screen 3. A label can be attached to the hand holding part 31 to facilitate the identification of the slice. In order to further clamp and fix the filter screen 3, a clamping protrusion 33 is provided on one side of the hand holding part 31 facing the liquid storage box 2. A card hole 21 is provided on the liquid storage box 2 to match the clamping protrusion 33.

[0028] In order to avoid excessive liquid in the liquid storage box 2 from causing the slice to be stuck to the side wall of the filter screen 3, a liquid leakage hole 22 is provided on the liquid storage box 2, which is preferably V-shaped. The liquid leakage hole 22 is arranged towards the liquid drainage groove 11. The liquid drainage groove 11 extends in any radial direction through the wet box placing box 1 (i.e. the liquid drainage groove 11 extends in the horizontal direction at the left or right side of the wet box placing box 1), so as to drain the overflowed water outside the wet box placing box 1.

[0029] As known, the box body is a structure with a bottom, no cover and a surrounding side wall. In order to facilitate the taking of the liquid storage box 2 and effectively clamp and place the liquid storage box 2, 1 / 2-2 / 3 of the height h of the liquid storage box 2 is equal to the wall height d of the liquid storage box 2. The application preferably uses a liquid storage box 2 with a wall height d of 2 / 3 of the height h of the liquid storage box 2.

[0030] In order to reduce the evaporation of the liquid in the liquid storage box 2, a box cover 4 is provided on the wet box placing box 1. Any two ends of the box cover 4 are provided with clamping strips 41. The wet box placing box 1 is provided with clamping grooves 12 matching the clamping strips 41 on both sides. A gap is left between the box cover 4 and the liquid storage box 2.

[0031] In order to facilitate the taking, a pressing type buckle 5 is further installed between the wet box placing box 1 and the box cover 4. A handle 6 is provided on the box cover 4.

[0032] Finally, it is explained that the above embodiments are only used to illustrate the technical solutions of the present application and are not limited. Although the present application has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solutions of the present application can be modified or replaced by equivalents without departing from the purpose and scope of the present application. The technical solutions of the present application should be covered in the scope of the claims of the present application.

Claims

1. A pipette-free immunohistochemistry device for liquid-based sample preparation, characterized by, Include: Wet box placement box (1), the liquid storage box (2) is independently arranged in a matrix in the wet box placement box (1), the liquid drainage groove (11) is arranged between each row of adjacent liquid storage boxes (2) in the wet box placement box (1), and the filter screen (3) is arranged on the liquid storage box (2).

2. The liquid-based sample preparation device of claim 1, wherein the device is an immunochemistry device. The filter screen (3) is provided with an anti-skid strip (32) on one side facing the liquid storage box (2).

3. The liquid-based sample preparation device of claim 2, wherein the device is an immunochemistry device. The filter screen (3) is provided with a hand-held part (31) on the end face, and the hand-held part (31) is provided with a clamping position convex point (33) on one side facing the liquid storage box (2), and the liquid storage box (2) is provided with a clamping opening (21) matched with the clamping position convex point (33).

4. The liquid transfer immunohistochemistry device for liquid-based sample preparation of claim 1, wherein, The liquid storage box (2) is provided with a liquid leakage opening (22), and the liquid leakage opening (22) is arranged towards the liquid drainage groove (11), and the liquid drainage groove (11) extends through the wet box placement box (1) in any radial direction.

5. The liquid-based sample preparation device of claim 1, wherein the device is an immunochemistry device. 1 / 2-2 / 3 of the height h of the liquid storage box (2) is equal to the wall height d of the liquid storage box (2).

6. A pipette-based immunohistochemistry device for liquid-based sample preparation as claimed in claim 5, wherein, The wet box placement box (1) is provided with a box cover (4), and any two ends of the box cover (4) are provided with a clamping strip (41), and the two sides of the wet box placement box (1) are provided with a clamping groove (12) matched with the clamping strip (41), and the box cover (4) and the liquid storage box (2) are provided with a gap.

7. A liquid transfer immunohistochemistry device for liquid based sample preparation as claimed in claim 6, wherein, The wet box placement box (1) and the box cover (4) are further provided with a pressing type buckle (5), and the box cover (4) is provided with a handle (6).

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