Non-specific immunoglobulin detection kit for cattle and sheep
By designing a non-specific immunoglobulin detection kit for cattle and sheep and using turbidimetric method for semi-quantitative detection, the problem of insufficient antibodies in newborn calves and lambs was solved, their disease resistance was improved, and the morbidity and mortality rates were reduced.
Patent Information
- Application Number
- CN202423037213.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-10
- Publication Date
- 2025-11-18
- Estimated Expiration
- 2034-12-10
AI Technical Summary
Current technology lacks dedicated equipment for detecting the levels of nonspecific immunoglobulins in newborn calves and lambs, resulting in poor disease resistance and increased morbidity and mortality.
A bovine and ovine nonspecific immunoglobulin detection kit is provided, comprising a box, a centrifuge tube holder, centrifuge tubes and a Pasteur pipette, for semi-quantitative detection by turbidimetric method to evaluate the passive conversion effect of maternal antibodies in young animals.
It enables semi-quantitative detection of non-specific immunoglobulins in the serum of newborn calves and lambs, allowing for timely adjustments to feeding management, enhanced disease resistance, and reduced morbidity and mortality.
Smart Images
Figure CN223565714U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to the field of livestock feeding technology, especially to a cattle and sheep non-specific immunoglobulin detection kit. BACKGROUND
[0002] Newborn calves and newborn lambs are less stimulated by the outside world due to incomplete development of the immune system, and the antibody content and antibody types in the body are very few, and the disease resistance is poor, so it is necessary to obtain non-specific immunoglobulin from the mother through the umbilical cord and obtain non-specific immunoglobulin from colostrum, and enhance the resistance of young livestock through passive immunization. If newborn calves and lambs cannot obtain enough maternal antibodies from the mother, the content of non-specific immunoglobulin in the body will increase the morbidity and mortality of newborn calves and lambs, thereby causing serious economic losses. Whether newborn calves and newborn lambs can fully absorb and transform the non-specific immunoglobulin obtained from the mother to achieve successful passive transformation of maternal antibodies and achieve the desired disease resistance, the serum immunoglobulin of newborn young livestock needs to be detected. At present, there is no special detection device, so it is an urgent technical problem for those skilled in the art to provide a cattle and sheep non-specific immunoglobulin detection device. SUMMARY
[0003] The utility model discloses a cattle and sheep non-specific immunoglobulin detection kit to solve the problems existing in the prior art, which is used for detecting non-specific immunoglobulin in the serum of newborn calves and lambs, and can evaluate the passive transformation effect of maternal antibodies of young livestock through semi-quantitative turbidimetry.
[0004] To achieve the above object, the utility model provides the following scheme:
[0005] The utility model provides a cattle and sheep non-specific immunoglobulin detection kit, which comprises a box body, a centrifugal tube bracket, a centrifugal tube one, a Pasteur pipette and a centrifugal tube two arranged in the box body, the centrifugal tube bracket is used for supporting the centrifugal tube one and the centrifugal tube two, the centrifugal tube one is used for containing sodium sulfate sterile solution, the Pasteur pipette is used for sucking the serum sample to be detected and is provided with a plurality of Pasteur pipettes, and the centrifugal tube two is used for containing successfully passively transformed serum control samples and passively transformed serum control samples respectively.
[0006] Preferably, the box body is a rectangular paper box.
[0007] Preferably, the centrifugal tube bracket is provided with three centrifugal tube brackets, and the three centrifugal tube brackets are stacked in the box body in the height direction.
[0008] Preferably, three rows of centrifuge tube insertion holes are arranged on the centrifuge tube bracket, the centrifuge tubes are inserted into the centrifuge tube insertion holes, the same row of centrifuge tubes contain the same concentration of sodium sulfate sterile solution, and different concentrations of sodium sulfate sterile solution are contained in the centrifuge tubes of each row.
[0009] Preferably, centrifuge tube insertion holes two are further arranged on one of the centrifuge tube brackets, and centrifuge tubes two containing successfully passively transformed serum control samples and passively transformed failed serum control samples are inserted into the centrifuge tube insertion holes two.
[0010] Preferably, the several Pasteur pipettes are contained in a transparent plastic bag.
[0011] Preferably, the volume of the Pasteur pipette is greater than 0.1ml, and a 0.1ml mark is arranged on the tube body.
[0012] Preferably, the centrifuge tubes one are high-transparency polypropylene tubes, the tube covers of the centrifuge tubes one are provided with explosion-proof buckles, and the tube covers of the centrifuge tubes one of the same row are marked with a color dot sticker.
[0013] Preferably, the centrifuge tubes two are brown polypropylene tubes, and the tube covers of the centrifuge tubes two are provided with explosion-proof buckles.
[0014] Preferably, the centrifuge tube bracket is a rectangular black EPE pearl cotton block.
[0015] The utility model discloses the following technical effects are obtained relative to prior art:
[0016] For non-specific immunoglobulin detection in serum of newborn calves and lambs, semi-quantitative turbidimetry is carried out, passive transformation effect of maternal antibody of young livestock can be evaluated, feeding management scheme of young livestock with passive transformation failure of maternal antibody is adjusted in time, passive immunization capacity is enhanced, disease resistance is improved, and morbidity and mortality of newborn calves and newborn lambs are reduced. BRIEF DESCRIPTION OF DRAWINGS
[0017] In order to more clearly illustrate the technical scheme in the embodiments of the utility model or prior art, the drawings needed in the embodiments will be briefly introduced as follows, and obviously, the drawings in the following description are only some embodiments of the utility model, and other drawings can be obtained according to these drawings without creative labor for those skilled in the art.
[0018] Fig. 1 It is a structural schematic view of the non-specific immunoglobulin detection kit for cattle and sheep in the utility model;
[0019] Fig. 2 It is a structural schematic view of the large centrifuge tube bracket in the utility model.
[0020] Fig. 3 It is a structure schematic view of the small centrifugal tube bracket in the utility model;
[0021] In the figure: 1, box body; 2, centrifugal tube bracket; 3, centrifugal tube one; 4, centrifugal tube two; 5, centrifugal tube insertion hole one; 6, centrifugal tube insertion hole two. DETAILED DESCRIPTION
[0022] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.
[0023] The utility model aims at providing a kind of cattle and sheep non-specific immunoglobulin detection kit to solve the problems existing in prior art.
[0024] In order to make the above purposes, features and advantages of the utility model more apparent and easy to understand, the utility model will be further described in detail with reference to the drawings and specific embodiments.
[0025] The cattle and sheep non-specific immunoglobulin detection kit in the embodiment, as shown in Figs. 1-3 It includes box body 1 and the centrifugal tube bracket 2, centrifugal tube one 3, Pasteur pipette (not shown in the figure) and centrifugal tube two 4 arranged in box body 1, centrifugal tube bracket 2 is used to support centrifugal tube one 3 and centrifugal tube two 4, centrifugal tube one 3 is used to hold sodium sulfate sterile solution, Pasteur pipette is used to extract serum sample to be detected and is provided with several, centrifugal tube two 4 is used to hold successfully passive transformation serum control sample and passive transformation failure serum control sample respectively.
[0026] In the specific embodiment, box body 1 is rectangular cuboid packing carton, and the length of box body 1 is 20.5cm, the width is 9.5cm, and the height is 15cm.
[0027] In the specific embodiment, centrifugal tube bracket 2 is provided with 3, and is stacked in box body 1 according to height direction, and can be taken out from box body 1 when using.
[0028] In the embodiment, the centrifuge tube bracket 2 is provided with three rows of centrifuge tube insertion holes 5, and the centrifuge tubes 3 are inserted into the centrifuge tube insertion holes 5. The centrifuge tubes 3 in the same row contain the same concentration of sterile sodium sulfate solution, and the centrifuge tubes 3 in different rows contain different concentrations of sterile sodium sulfate solution. One of the centrifuge tube brackets 2 is a large bracket, and the centrifuge tube bracket 2 is further provided with centrifuge tube insertion holes 6. The centrifuge tubes 4 containing the successfully passively transformed serum control sample and the passively transformed serum control sample are inserted into the centrifuge tube insertion holes 6. The large centrifuge tube bracket 2 has a length of 20 cm, a width of 8.5 cm, and a thickness of 2.0 cm. The small centrifuge tube bracket 2 has a length of 20 cm, a width of 6.0 cm, and a thickness of 2.0 cm. The centrifuge tube bracket 2 is a rectangular black EPE pearl cotton block. Since white precipitates appear in the detection results, the black bracket forms a more obvious visual difference, and the results are easy to observe.
[0029] In the embodiment, a plurality of Pasteur pipettes are contained in a transparent plastic bag. The volume of the Pasteur pipette is greater than 0.1 ml, and the pipette body is provided with a 0.1 ml mark. The Pasteur pipette sucks 0.1 ml of the serum sample to be detected each time.
[0030] In the embodiment, the centrifuge tubes 3 are high-transparency polypropylene tubes, and the tube caps of the centrifuge tubes 3 are provided with explosion-proof buckles. The tube caps of the centrifuge tubes 3 in the same row are marked with a round dot sticker of one color, and the round dot sticker is marked with A, B, and C marks. The three rows are a row of A tubes, a row of B tubes, and a row of C tubes.
[0031] A tube (14% sodium sulfate solution tube): 1.5 ml transparent centrifuge tube containing 0.9 ml of 14% sterile sodium sulfate solution, a total of 30 tubes.
[0032] B tube (16% sodium sulfate solution tube): 1.5 ml transparent centrifuge tube containing 0.9 ml of 16% sterile sodium sulfate solution, a total of 30 tubes.
[0033] C tube (18% sodium sulfate solution tube): 1.5 ml transparent centrifuge tube containing 0.9 ml of 18% sterile sodium sulfate solution, a total of 30 tubes.
[0034] In the embodiment, the centrifuge tubes 4 are brown polypropylene tubes, and the tube caps of the centrifuge tubes 4 are provided with explosion-proof buckles. The volume of the centrifuge tubes 4 is 1.5 ml, and the centrifuge tubes 4 contain 1.5 ml of the successfully passively transformed serum control sample and the passively transformed serum control sample. The purpose of the successfully passively transformed serum control sample and the passively transformed serum control sample is to have obvious characteristics when added to the sodium sulfate solution, and to provide a reference for the detection results of the serum sample to be detected.
[0035] The application of the bovine and sheep non-specific immunoglobulin detection kit in the application is as follows:
[0036] Mix the serum sample to be tested with 14%, 16%, 18% sodium sulfate solution respectively, and after standing, the serum immunoglobulin content is different, and the different concentrations of sodium sulfate-serum mixture presents different turbidity degree, and the immunoglobulin is semi-quantified by judging the turbidity degree.
[0037] 0.1ml of the serum sample to be tested is added into the centrifuge tube containing 14%, 16%, 18% sodium sulfate solution respectively by using a Pasteur pipette, mixed, and stood at room temperature (20℃-25℃) for 1h.
[0038] 0.1ml of the serum sample to be tested is added into the centrifuge tube containing 14%, 16%, 18% sodium sulfate solution respectively by using a Pasteur pipette, mixed, and stood at room temperature (20℃-25℃) for 1h.
[0039] 0.1ml of the serum sample to be tested is added into the centrifuge tube containing 14%, 16%, 18% sodium sulfate solution respectively by using a Pasteur pipette, mixed, and stood at room temperature (20℃-25℃) for 1h.
[0040] Result interpretation:
[0041]
[0042] The principle and implementation mode of the specific examples are described in the utility model, and the above embodiment is only used for helping to understand the method and core idea of the utility model; meanwhile, for the general technical personnel in the field, according to the idea of the utility model, there will be changes in the specific implementation mode and application range. In conclusion, the content of the specification should not be understood as the limitation of the utility model.
Claims
1. A bovine and ovine non-specific immunoglobulin detection kit, characterized in that: The box body and the centrifugal tube bracket, the centrifugal tube one, the Pasteur pipette and the centrifugal tube two arranged in the box body, the centrifugal tube bracket is used for supporting the centrifugal tube one and the centrifugal tube two, the centrifugal tube one is used for containing sodium sulfate sterile solution, the Pasteur pipette is used for sucking serum sample to be detected and is arranged with several, the centrifugal tube two is used for containing respectively successfully passive transformation serum control sample and passive transformation failure serum control sample.
2. The bovine and ovine non-specific immunoglobulin detection kit according to claim 1, characterized in that: The box body is a rectangular carton.
3. The bovine and ovine non-specific immunoglobulin detection kit according to claim 1, characterized in that: The centrifugal tube bracket is arranged with three and is stacked in the box body in the height direction.
4. The bovine and ovine non-specific immunoglobulin detection kit according to claim 3, characterized in that: Three rows of centrifugal tube insertion holes one are arranged on the centrifugal tube bracket, the centrifugal tube one is inserted into the centrifugal tube insertion hole one, the same row of each centrifugal tube one is filled with sodium sulfate sterile solution with the same concentration, and the centrifugal tube one in each row is filled with sodium sulfate sterile solution with different concentrations.
5. The bovine and ovine non-specific immunoglobulin detection kit according to claim 3, characterized in that: One of the centrifugal tube brackets is further provided with centrifugal tube insertion hole two, and the centrifugal tube two containing successfully passive transformation serum control sample and passive transformation failure serum control sample is inserted into the centrifugal tube insertion hole two.
6. The bovine and ovine non-specific immunoglobulin test kit according to claim 1, characterized in that: The several Pasteur pipettes are contained in a transparent plastic bag.
7. The bovine and ovine non-specific immunoglobulin test kit according to claim 6, characterized in that: The volume of the Pasteur pipette is greater than 0.1ml, and a 0.1ml mark is arranged on the pipe body.
8. The bovine and ovine non-specific immunoglobulin test kit according to claim 1, characterized by: The centrifugal tube one is a high-transparency polypropylene tube, the cover of the centrifugal tube one is provided with an anti-explosion buckle, and the covers of the centrifugal tubes in the same row are marked with a color dot sticker.
9. The bovine and ovine non-specific immunoglobulin test kit according to claim 1, characterized by: The centrifugal tube two is a brown polypropylene tube, and the cover of the centrifugal tube two is provided with an anti-explosion buckle.
10. The bovine and ovine non-specific immunoglobulin test kit according to claim 1, characterized in that: The centrifugal tube bracket is a rectangular black EPE pearl wool block.