Microscope for detecting observation window of fermentation tank

Abstract

The utility model discloses a microscope for detecting an observation window of a fermentation tank, which comprises the observation window, a receiver arranged at the side part of the observation window, a microscope assembly arranged between the observation window and the receiver and comprising a microscope objective group positioned at the side part of the observation window, the microscope objective group is used for increasing the working distance and increasing the numerical aperture of the system; and the reflector group is located at the side part of the microscope objective group, and the reflector group is used for ensuring the light passing quantity participating in imaging. Through mutual cooperation of the first lens, the second lens, the third lens, the secondary mirror and the primary mirror, light is emitted from the first lens, then enters the surface of the primary mirror, is emitted to the secondary mirror through the primary mirror, is reflected on the secondary mirror and is imaged on the sensing surface of the receiver, so that an object space medium is changed, and the imaging quality of the receiver is improved. The high resolution ratio of detection of the outer surface and the inner surface of the observation window of the cell culture tank is conveniently improved, so that the interior of the culture tank is observed and operated.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of microscope, especially a microscope for the observation window detection of fermentation tank. BACKGROUND

[0002] In order to realize the non-contact measurement of cell culture tank without taking liquid, it is necessary to erect a long working distance microscope outside the cell culture tank to observe the cells in the cell culture tank, which requires that the inner and outer walls of the observation window of the cell culture tank are clean enough and have no scratches, pits and the like that affect observation, and then a microscope needs to be used.

[0003] The target observed by the prior art industrial detection microscope is about 5mm, and the detection precision is low, so it can only detect the surface of the measured object, and thus cannot observe the inner surface condition through the 18mm observation window of the cell culture tank, resulting in that the observation operation cannot be carried out, and there is certain limitation. UTILITY MODEL CONTENT

[0004] The utility model discloses a microscope for the observation window detection of fermentation tank to solve the problem in the background art.

[0005] In order to achieve the above purpose, the utility model provides the following technical scheme: a microscope for the observation window detection of fermentation tank, comprising:

[0006] The observation window is provided with a receiver on the side;

[0007] The microscope assembly is arranged between the observation window and the receiver, and the microscope assembly comprises:

[0008] The microscope objective group is arranged on the side of the observation window, and is used for increasing the working distance and increasing the system numerical aperture;

[0009] The mirror group is arranged on the side of the microscope objective group, and is used for ensuring the light throughput participating in imaging.

[0010] Preferably, the microscope objective group comprises:

[0011] The lens double cemented lens group has a negative focal length;

[0012] The first lens is arranged on the side of the lens double cemented lens group, and the first lens and the lens double cemented lens group are used for adjusting the primary phase difference, so that the microscope system obtains high-quality image quality.

[0013] Preferably, the lens double cemented lens group comprises:

[0014] The second lens is arranged close to the side of the observation window;

[0015] A third lens is fixedly connected to the side of the second lens.

[0016] Preferably, the mirror group comprises:

[0017] A main mirror is located on the side of the receiver.

[0018] A secondary mirror is located between the main mirror and the first lens.

[0019] Preferably, the main mirror and the secondary mirror are both provided with a reflective layer, and the reflective layer is made of a metal reflective film material.

[0020] Preferably, a blocking ratio is provided between the main mirror and the secondary mirror, and the value of the blocking ratio is 0.5.

[0021] The technical effects and advantages of the present application are as follows:

[0022] The first lens, the second lens, the third lens, the secondary mirror and the main mirror are cooperated with each other, the first lens, the second lens and the third lens increase the working distance and the system digital aperture, the light is emitted from the first lens, is incident to the surface of the main mirror, is emitted from the main mirror to the secondary mirror, is reflected on the secondary mirror, and is imaged on the sensing surface of the receiver, thereby being beneficial to change the object medium, and being beneficial to improve the high resolution of the outer surface and the inner surface detection of the cell culture tank observation window, so that the inside of the culture tank can be observed and operated. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 The present application is a microscope structure schematic diagram.

[0024] Figure 2 The present application is a microscope working light schematic diagram.

[0025] Figure 3 The present application is a microscope receiver position schematic diagram when detecting the culture tank.

[0026] Figure 4 The present application is a microscope MTF curve diagram when observing the 10L culture tank.

[0027] Figure 5 The present application is a microscope MTF curve diagram when observing the 30L culture tank.

[0028] Figure 6 The present application is a microscope MTF curve diagram when observing the 50L culture tank.

[0029] In the figure: 1, observation window; 2, receiver; 3, microscope assembly; 301, first lens; 302, second lens; 303, third lens; 304, secondary mirror; 305, main mirror. DETAILED DESCRIPTION

[0030] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.

[0031] The utility model provides a kind of microscope for fermenter observation window detection as Figures 1-6 As shown in a kind of microscope for fermenter observation window detection, including observation window 1, the side of observation window 1 is provided with receiver 2, receiver 2 can be CCD or CMOS, receiver 2 is favorable to the reception of light, so that it carries out imaging operation, receiver 2 installs sleeve and microscope sleeve, the position of receiver 2 is adjusted by rotating adjusting ring, so that receiver is located in imaging focal plane position.

[0032] Further, observation window 1 and receiver 2 are provided with microscope assembly 3, the working distance of microscope assembly 3 is 28mm, object field of view is 30mm, numerical aperture is 0.2 and resolution is 1.68 microns, change object medium, can realize the high-resolution microscope of cell culture tank observation window outer surface and inner surface detection, microscope focal plane places receiver 2, microscope assembly 3 includes micro-object lens group and mirror group, micro-object lens group is located in the side of observation window 1, and micro-object lens group is used to increase working distance and increase system numerical aperture, and mirror group is located in the side of micro-object lens group, and mirror group is used to ensure the light throughput participating in imaging.

[0033] Specifically, micro-object lens group includes lens double cemented lens group and first lens 301, the combined focal length of lens double cemented lens group is negative focal value, first lens 301 is located in the side of lens double cemented lens group, and first lens 301 and lens double cemented lens group are used to adjust primary phase difference, so that microsystem obtains high-quality image quality.

[0034] It should be noted that lens double cemented lens group includes second lens 302 and third lens 303, second lens 302 is close to the side of observation window 1, and third lens 303 is fixedly connected to the side of second lens 302.

[0035] The imaging element data table of first lens 301, second lens 302, third lens 303, main mirror 305 and secondary mirror 304 is as follows:

[0036]

[0037] Specifically, the mirror group includes a primary mirror 305 and a secondary mirror 304, the light is emitted from the first lens 301 and then incident on the surface of the primary mirror 305, and then the light is emitted on the secondary mirror 304 by the metal reflecting film on the surface of the primary mirror 305, and then the light is reflected on the metal reflecting film on the secondary mirror 304 and then imaged on the photosensitive surface of the receiver 2, the primary mirror 305 is located on the side of the receiver 2, the secondary mirror 304 is located between the primary mirror 305 and the first lens 301, the primary mirror 305 and the secondary mirror 304 are both provided with a reflecting layer, the reflecting layer is a metal reflecting film material, and a blocking ratio is provided between the primary mirror 305 and the secondary mirror 304, the value of the blocking ratio is 0.5, thereby facilitating to ensure the light quantity participating in imaging.

[0038] In order to adapt to the observation windows 1 of different models of cell culture tanks, three positions of the receiver 2 are provided for 10L, 30L and 50L, as shown in Figure 3 from top to bottom, corresponding to 50L, 30L and 10L, and the observation results are as shown in Figure 3 Since the curvatures of the observation windows 1 of the 10L, 30L and 50L culture tanks are different, as shown in Figure 4 , Figure 5 and Figure 6 , it is concluded that the medium for microscope observation is changed from air to the glass of the observation window 1, resulting in that the focal plane positions of the images are also different when the observation windows 1 of different models of culture tanks are detected.

[0039] Finally, it should be pointed out that: the above only describes preferred embodiments of the present application and is not used to limit the present application, although the present application has been described in detail with reference to the foregoing embodiments, for those skilled in the art, the technical solutions recorded in the foregoing embodiments can be modified, or some technical features can be replaced, any modification, equivalent replacement, improvement, etc. within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims

1. A microscope for fermenter observation window detection, comprising: an observation window (1), the side of the observation window (1) being provided with a receiver (2); characterized in that a microscope assembly (3) is arranged between the observation window (1) and the receiver (2), the microscope assembly (3) comprising: a microscope objective group, the microscope objective group being arranged at the side of the observation window (1), the microscope objective group being used to increase the working distance and increase the system numerical aperture; a mirror group, the mirror group being arranged at the side of the microscope objective group, the mirror group being used to ensure the light throughput involved in imaging.

2. A microscope for fermenter sight glass inspection according to claim 1, characterized in that, The microscope objective group comprises: a lens double cemented lens group, the combined focal length of the lens double cemented lens group being a negative focal length; a first lens (301), the first lens (301) being arranged at the side of the lens double cemented lens group, the first lens (301) and the lens double cemented lens group being used to adjust the primary phase difference, so that the microscopic system obtains high-quality image quality.

3. A microscope for fermenter sight glass inspection according to claim 2, wherein, The lens double cemented lens group comprises: a second lens (302), the second lens (302) being arranged close to the side of the observation window (1); a third lens (303), the third lens (303) being fixedly connected to the side of the second lens (302).

4. A microscope for fermenter sight glass inspection according to claim 1, wherein, The mirror group comprises: a primary mirror (305), the primary mirror (305) being arranged at the side of the receiver (2); a secondary mirror (304), the secondary mirror (304) being arranged between the primary mirror (305) and the first lens (301).

5. A microscope for fermenter sight glass inspection according to claim 4, wherein, The primary mirror (305) and the secondary mirror (304) are both coated with a light-reflecting layer, and the light-reflecting layer is a metal reflective film material.

6. A microscope for fermenter sight glass inspection according to claim 4, wherein, A blocking ratio is arranged between the primary mirror (305) and the secondary mirror (304), and the value of the blocking ratio is 0.5.

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