Noninvasive single-side or double-side uterus injection device for experiment

Abstract

A noninvasive single-side or double-side uterus injection device for an experiment comprises an intrauterine catheter part, an injector connecting part, a connecting part sealing cover, an intrauterine catheter folding fixator and an intrauterine catheter clamp. One end of the injector connecting part is connected with the intrauterine catheter part, the other end of the injector connecting part is used for being connected with an injector, and the connecting part sealing cover is detachably installed at the other end of the injector connecting part; the uterine cavity inner catheter part is composed of a first catheter and a second catheter, the first catheter and the second catheter are arranged side by side and used for dosing of different uterine cavities respectively, and the uterine cavity inner catheter clamp and the uterine cavity catheter folding fixator are connected to the outer side of the uterine cavity inner catheter part in a sliding and sleeving mode. And the uterine cavity catheter folding fixer is farther from the syringe connecting part than the uterine cavity inner catheter clip. According to the utility model, unilateral / bilateral uterus modeling can be carried out noninvasively and selectively through the vagina, secondary laparotomy is avoided, the stress, infection and death risks of model animals are reduced, and the utility model belongs to the technical field of medical equipment.

Description

TECHNICAL FIELD

[0001] The utility model relates to medical equipment technical field, concretely relates to a noninvasive unilateral or bilateral uterine injection device for experiment. BACKGROUND

[0002] Uterine adhesion (IUA) is caused by abortion, uterine operation and uterine surgery, and infection is the inducement of promoting IUA progression and recurrence. Because the endometrium of IUA patient is thin, uterine cavity is adhesion and cicatrization, lead to uterine cavity stenosis even occlusion, and the clinical performance is little menstruation, infertility even abortion, is the main cause of female uterine infertility, also is the terminal bottleneck of implementing assisted reproductive technology. The incidence of serious obstetric complications such as abortion, premature delivery, placenta implantation of IUA patient is also extremely high, seriously threatens the life safety of mother and child. Effectively treating IUA, improving uterine receptivity and pregnancy rate, improving pregnancy outcome become the research emphasis in the field of female reproductive health and reproductive medicine.

[0003] Good animal model is the favorable tool of researching the pathogenesis of IUA, exploring new treatment method and carrying out preclinical in vivo research. At present, the model animals of IUA are mostly small mammals such as rats, mice and new zealand white rabbits. The three kinds of double uterus animals, we can implement bilateral uterine modeling also can implement unilateral uterine modeling. Rat has the advantages such as easy to raise, easy to anesthetize, convenient for operation, saves the cost of feeding and experimental cost. From the economic cost and experimental convenience, the model animal is rat best. The pregnancy of experimental rat is also affected by other interference factors, for example: stress, too small gestational age or failure of caging can also cause infertility of rat. One side uterine modeling and the normal uterus of opposite side as its own control, then is favorable to analyze the interference factors of pregnancy experimental result, and the result of experimental intervention is more real.

[0004] The existing rat modeling methods are mainly divided into the following types: chemical injury modeling method, mechanical injury modeling method (or scraping modeling method), infection (lipopolysaccharide LPS uterine injection) modeling method and double injury (mechanical injury + infection) modeling method. The modeling method of mechanical injury of rat uterus is to expose the rat uterus by laparotomy surgery, and then scrape one side / bilateral uterus with a spatula to complete. Although the modeling method of mechanical injury can simulate the damage of intrauterine surgical operation to the endometrium in clinical female, it is reported in the literature that due to the strong self-repairing ability of rat uterus, the mechanical injury of rat uterus caused by spatula can be completely repaired after two estrus cycles, so this modeling method is not reliable for evaluating the therapeutic effect of experimental intervention. Our animal experiment research also found that the pregnancy rate of the non-intervention group (i.e. the control group) of rats was still 40% when using the mechanical injury method alone to construct the IUA rat model, indicating that the uterine function of a considerable part of the modeling rats had completely recovered. This also caused the embarrassing situation that the pregnancy rate of the non-intervention group of rats was difficult to form a significant difference with that of the experimental intervention group of rats, which could not reflect the real effect of experimental intervention and was not conducive to exploring new treatment programs.

[0005] The modeling method of double injury (mechanical injury + infection) is to combine the mechanical injury of the uterine endometrium of the rat by conventional scratching with the injection of lipopolysaccharide (LPS) into the uterus of the rat. LPS is also known as endotoxin, which is a component of the outer wall of the cell wall of gram-negative bacteria and can cause inflammatory response of the body. The injection of LPS into the uterus of the rat causes the infection of the uterine cavity, which is a method for constructing the rat model of uterine infection. The pathological changes of the infection in the uterus of the rat can be observed under a microscope after 24 hours. The modeling method of double injury is more close to the pathogenesis of clinical IUA, and can construct the rat model of IUA conforming to the clinical pathology, and thus is more popular. There are two ways for injecting LPS into the uterus of the rat, namely, through the vagina and through the abdomen. The injection of LPS into the uterus of the rat through the vagina does not need surgery, but it is difficult to inject the drug into the vagina due to the easy collapse of the vaginal wall of the rat, and the drug solution is easy to leak, which causes a large difference between the actual amount of the drug entering the uterus and the planned amount, and affects the experimental effect. In addition, the LPS injected into the vagina will be shunted to the bilateral uterine cavities, and the bilateral uteri will be infected, so that the unilateral uterine modeling of the experimental rat as the normal control of itself cannot be realized, and thus other infertility factors of the rat cannot be found, which is easy to cause false negative results of the experiment and is not conducive to the judgment of the intervention effect of the experiment. The injection of LPS into the uterus of the rat through the abdominal route also needs to perform laparotomy on the rat, expose the uterine body of the rat, and then complete the injection of the drug into the uterine cavity by using a syringe to pierce into the unilateral / bilateral uterine cavity, which has surgical trauma. The commonly used modeling method of double injury needs to perform laparotomy on the rat to inject LPS into the uterus of the rat after 24 hours, and then perform laparotomy again to scratch the uterine cavity of the rat and perform subsequent intrauterine treatment. Not only the number of surgical operations and the operation time are increased, but also the risk of infection of the abdominal cavity or even the whole body of the experimental rat and the risk of death of the experimental rat are greatly increased, which is contrary to the "3R" principle of medical experimental animals. At present, there is no better modeling method of double injury and convenient non-invasive unilateral or bilateral uterine injection device for experiments.

[0006] In summary, it is necessary to design an experimental non-invasive unilateral or bilateral uterine injection device and optimize the experimental scheme of the rat model of IUA. Content of the utility model

[0007] In view of the technical problems in the prior art, the purpose of the utility model is to provide an experimental non-invasive unilateral or bilateral uterine injection device, which is mainly used for rodents and rabbits, can selectively perform unilateral / bilateral uterine modeling through the vagina without trauma, avoids secondary laparotomy, is beneficial to reducing the number of experiments, avoiding excessive trauma and pain of the experimental animals and reducing the risk of infection and death of the experimental animals, and also saves experimental time and reduces experimental cost to a certain extent.

[0008] In order to achieve the above object, the utility model discloses the following technical scheme: an experimental non-invasive unilateral or bilateral uterine injection device, including uterine cavity catheter part, syringe connecting part, connecting part cover, uterine cavity catheter folding fixer, uterine cavity catheter clamp, the one end of syringe connecting part is connected uterine cavity catheter part, and the other end of syringe connecting part is used for connecting syringe, and the inside of syringe connecting part communicates the inside of uterine cavity catheter part, and connecting part cover is detachably installed in the other end of syringe connecting part, uterine cavity catheter part is composed of first catheter and second catheter, first catheter and second catheter are arranged side by side and are used to administer to different uterine cavities respectively, uterine cavity catheter clamp and uterine cavity catheter folding fixer are slidably sleeved on the outside of uterine cavity catheter part, and uterine cavity catheter folding fixer is farther than uterine cavity catheter clamp from syringe connecting part, uterine cavity catheter folding fixer is used to fold first catheter and second catheter, and first catheter and second catheter are all flexible tubes, and uterine cavity catheter clamp is used to control the opening and closing of first catheter and second catheter.

[0009] The device can be used to inject medicine through the intrauterine tube, which is convenient to operate and beneficial to quickly placing the tube into the uterine cavity through the vagina, thereby improving the experimental efficiency. The first catheter and / or the second catheter can be closed by the uterine cavity catheter clamp, the flow direction of the injected medicine can be controlled to one side / two sides of the uterine cavity, and unilateral / bilateral intrauterine injection of medicine can be performed, so that the non-invasive unilateral intrauterine injection of LPS and other medicines is realized, the contralateral uterus is used as a self-control, and other factors interfering with the experimental results can be easily investigated. The intrauterine injection can be realized repeatedly without repeated surgical trauma, and the risk of stress, infection and death of the model animal is greatly reduced.

[0010] As a preferred, the uterine cavity catheter folding fixer is provided with a left hole and a right hole, and the first catheter and the second catheter can respectively slide through the left hole and the right hole.

[0011] As a preferred, the uterine cavity catheter folding fixer is in the shape of an elliptical cylinder, and the left hole and the right hole are both circular through holes and symmetrically arranged.

[0012] As a preferred, the uterine cavity catheter clamp is provided with a closed groove and an open groove in communication with each other, the closed groove is a strip-shaped through groove, the open groove is a rectangular through groove, and the size of the open groove is adapted to the size of the uterine cavity catheter part.

[0013] As a preferred, the rectangular through groove is provided with rounded corners at four corners.

[0014] As a preferred, the connecting part cover is composed of an outer cover part and a plunger part that can be inserted into the other end of the syringe connecting part, and the outer cover part is fixedly connected with the plunger part.

[0015] As a preferred, the plunger part is in the shape of a circular truncated cone.

[0016] As a kind of preference, the shape of the intrauterine catheter clamp is rectangular plate.

[0017] As a kind of preference, the end of the first catheter and the second catheter are both semispherical surface with middle opening.

[0018] As a kind of preference, the material of the connecting part cover is PVC, TPE, PE or PP, the material of the syringe connecting part and the intrauterine catheter folding fixer is hard plastic or metal, the material of the intrauterine catheter clamp is hard plastic or metal, and the material of the intrauterine catheter part is PVC, TPE, PE or PP.

[0019] Generally, the present application has the following advantages:

[0020] (1) The device can realize non-invasive unilateral / bilateral intrauterine drug injection through the first catheter and the second catheter which can be controlled to open and close.

[0021] (2) The device is simple and convenient to operate for bilateral intrauterine drug injection experiment, and has no secondary surgical trauma.

[0022] (3) The device is made of safe and non-toxic materials, and has low production cost, which is suitable for large-scale industrial production.

[0023] (4) The device can be used as disposable experimental equipment after sterilization by ethylene oxide, which can effectively avoid uterine or systemic infection during or after experimental modeling. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 It is a structural schematic view of an experimental non-invasive unilateral or bilateral uterine injection device.

[0025] Figure 2 It is a connection schematic view of the intrauterine catheter part, the syringe connecting part and the connecting part cover.

[0026] Figure 3 It is a sectional schematic view of the intrauterine catheter folding fixer.

[0027] Figure 4 It is a sectional schematic view of the intrauterine catheter clamp.

[0028] Among them, 1 is the intrauterine catheter part; 11 is the first catheter, and 12 is the second catheter; 2 is the syringe connecting part; 3 is the connecting part cover; 4 is the intrauterine catheter folding fixer; 41 is the left hole, and 42 is the right hole; 5 is the intrauterine catheter clamp; 51 is the closed groove; and 52 is the open groove. DETAILED DESCRIPTION

[0029] The present application will be further described in detail below in combination with the drawings and specific embodiments.

[0030] Example 1

[0031] like Figures 1-2 As shown, an experimental non-invasive unilateral or bilateral uterine injection device includes an intrauterine catheter section, a syringe connection section, a connection section cap, an intrauterine catheter closing and fixing device, and an intrauterine catheter clamp. One end of the syringe connection section is connected to the intrauterine catheter section, and the other end of the syringe connection section is used to connect to a syringe. The interior of the syringe connection section communicates with the interior of the intrauterine catheter section. The connection section cap is detachably installed on the other end of the syringe connection section. The intrauterine catheter section consists of a first catheter and a second catheter, which are arranged side by side and are used to administer medication to different uterine cavities. The intrauterine catheter clamp and the intrauterine catheter closing and fixing device are slidably sleeved on the outside of the intrauterine catheter section. The intrauterine catheter closing and fixing device is farther from the syringe connection section than the intrauterine catheter clamp. The intrauterine catheter closing and fixing device is used to close the first catheter and the second catheter. Both the first catheter and the second catheter are soft tubes. The intrauterine catheter clamp is used to control the opening and closing of the first catheter and the second catheter.

[0032] like Figure 3 As shown, the uterine cavity catheter closing and fixing device has a left hole and a right hole, and the first catheter and the second catheter can slide through the left hole and the right hole, respectively.

[0033] The uterine cavity catheter closing and fixing device is elliptical cylindrical, with both the left and right orifices being circular and symmetrically arranged. The size of the left orifice is adapted to the size of the first catheter, and the size of the right orifice is adapted to the size of the second catheter.

[0034] like Figure 4 As shown, the intrauterine catheter clamp has interconnected closed grooves and open grooves. The closed groove is a strip-shaped through groove, and the open groove is a rectangular through groove. The size of the open groove is adapted to the size of the intrauterine catheter section.

[0035] The intrauterine catheter portion passes through a closed groove and / or an open groove. The size of the open groove is slightly larger than the overall size of the intrauterine catheter portion. When both the first and second intrauterine catheters are located in the open groove, both catheters are in an open state. However, when the first and / or second catheters are stuck in the closed groove, the narrower width of the closed groove causes the first and / or second catheters to be flattened and cut off. Therefore, unilateral / bilateral drug delivery control can be achieved using intrauterine catheter clamps.

[0036] The four corners of the rectangular through groove are rounded. This rounded corner design allows the first and second conduits to move smoothly within the rectangular through groove without easily getting stuck.

[0037] The connecting part cover is composed of an outer cover part and a plunger part which can be inserted into the other end of the syringe connecting part, and the outer cover part is fixedly connected with the plunger part. By inserting the plunger part into the other end of the syringe connecting part, the syringe connecting part can be sealed, and the plunger part can be pulled out during use, thus being convenient to operate.

[0038] The plunger part is in the shape of a circular truncated cone. The plunger part in the shape of a circular truncated cone can be more convenient to insert and pull out.

[0039] The uterine cavity catheter clamp is in the shape of a rectangular plate.

[0040] The ends of the first catheter and the second catheter are both in the shape of a semispherical surface with a hole in the middle. By the semispherical surface, the rat's vagina can not be easily damaged when the device is pushed in, and the operation can be realized without damage.

[0041] The material of the connecting part cover is PVC, TPE, PE or PP, the material of the syringe connecting part and the uterine cavity catheter folding fixer is hard plastic or metal, the material of the uterine cavity catheter clamp is hard plastic or metal, and the material of the uterine cavity catheter part is PVC, TPE, PE or PP.

[0042] The uterine cavity catheter clamp is made of PC, ABS, PE, PP or metal, and the uterine cavity catheter part is made of PP. The uterine cavity catheter part is preferably made of high molecular polypropylene material.

[0043] The use process of the device is as follows, taking a rat as an example: first, push the catheter folding fixer which is slidably arranged outside the uterine cavity catheter part to the top end close to the uterine cavity catheter part, so that the bilateral uterine cavity catheter parts are folded, then gently push the device into the rat's vagina, when the uterine cavity catheter part reaches the top end of the vagina, resistance is felt, at this time, slowly slide out the catheter folding fixer with the left hand, at this time, the ends of the first catheter and the second catheter are separated, hold the device with the right hand and continue to send it into the uterine cavity, so that the first catheter and the second catheter are pushed into the left and right uterine cavities respectively, and then slightly slide outwards when resistance is encountered again. Remove the connecting part cover, connect the syringe, push the LPS medicine into the uterus, and the medicine is injected into the bilateral uterine cavities. After staying for 5 minutes, pull out the device from the rat's body. After 24 hours, the model rat can be opened and the uterine scraping and uterine treatment experiment can be performed according to the conventional method. The device is used to construct a double-injury IUA model in the bilateral uterus of a rat, which is not only simple, convenient and efficient, but also has no secondary surgical trauma.

[0044] Example Two

[0045] Using the experimental non-invasive unilateral or bilateral uterine injection device in Example One, take rats as an example: first push the catheter folding fixer outside the catheter part in the rat uterine cavity to the top of the catheter part in the uterine cavity, so that the bilateral catheter parts in the uterine cavity are folded, and gently push them into the rat vagina. When the catheter reaches the top of the vagina, there is a sense of resistance. At this time, slowly slide the catheter folding fixer outwards with the left hand. At this time, the ends of the first catheter and the second catheter are separated, and the right hand holds the device and continues to send it into the uterine cavity, so that the first catheter and the second catheter are pushed into the left and right uterine cavities, respectively. When resistance is encountered again, slightly withdraw it outwards and fix it. Use the uterine cavity catheter clamp to clamp the catheter in one side of the uterine cavity. After removing the connecting part cover, connect the syringe, push the LPS and other drugs into the uterus, and the drugs are injected into the unilateral uterine cavity. After staying for 5 minutes, withdraw the device from the rat body. After 24 hours, perform uterine scraping and uterine treatment experiments on the model rats according to the conventional method. The device can be used to complete unilateral uterine modeling that cannot be achieved by other transvaginal injection methods. The unilateral uterine double-damage IUA model is constructed, and one side of the uterine cavity of the experimental rat is used as a self-control, which can eliminate the interference factors of rat infertility such as too small gestational age or failure of cage mating, so that the intervention experiment result is true and reliable.

[0046] The above examples are the preferred embodiments of the utility model, but the embodiments of the utility model are not limited by the above examples, and any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the utility model should be equivalent replacement methods, which are all included in the protection scope of the utility model.

Claims

1. A non-invasive unilateral or bilateral uterine injection device for experimental use, characterized in that: It includes an intrauterine catheter portion, a syringe connector, a connector cap, an intrauterine catheter closing and fixing device, and an intrauterine catheter clamp; one end of the syringe connector is connected to the intrauterine catheter portion, and the other end of the syringe connector is used to connect to the syringe; the interior of the syringe connector communicates with the interior of the intrauterine catheter portion; the connector cap is detachably installed on the other end of the syringe connector. The intrauterine catheter section consists of a first catheter and a second catheter. The first and second catheters are arranged side by side and are used to administer drugs to different uterine cavities. The intrauterine catheter clamp and the intrauterine catheter closing and fixing device are slidably sleeved on the outside of the intrauterine catheter section. The intrauterine catheter closing and fixing device is farther from the syringe connection than the intrauterine catheter clamp. The uterine cavity catheter closing and fixing device is used to close the first catheter and the second catheter. Both the first catheter and the second catheter are soft tubes. The uterine cavity catheter clamp is used to control the opening and closing of the first catheter and the second catheter. The uterine cavity catheter closing and fixing device has a left hole and a right hole, and the first catheter and the second catheter can slide through the left hole and the right hole respectively.

2. The experimental non-invasive unilateral or bilateral uterine injection device according to claim 1, characterized in that: The uterine cavity catheter closing and fixing device is elliptical cylindrical, with both the left and right holes being circular through holes and symmetrically arranged.

3. The experimental non-invasive unilateral or bilateral uterine injection device according to claim 1, characterized in that: The intrauterine catheter clamp has interconnected closed and open slots. The closed slot is a strip-shaped through slot, and the open slot is a rectangular through slot. The size of the open slot is adapted to the size of the intrauterine catheter section.

4. An experimental non-invasive unilateral or bilateral uterine injection device according to claim 3, characterized in that: The four corners of the rectangular through groove are rounded.

5. An experimental non-invasive unilateral or bilateral uterine injection device according to claim 1, characterized in that: The connector cap consists of an outer cover and a plunger that can be inserted into the other end of the syringe connector, with the outer cover being fixedly connected to the plunger.

6. An experimental non-invasive unilateral or bilateral uterine injection device according to claim 5, characterized in that: The plunger section is truncated cone-shaped.

7. An experimental non-invasive unilateral or bilateral uterine injection device according to claim 1, characterized in that: The uterine cavity catheter clamp is rectangular in shape.

8. An experimental non-invasive unilateral or bilateral uterine injection device according to claim 1, characterized in that: The ends of both the first and second catheters are hemispherical surfaces with a central opening.

9. An experimental non-invasive unilateral or bilateral uterine injection device according to claim 1, characterized in that: The material of the connector cap is PVC, TPE, PE or PP; the material of the syringe connector and the uterine cavity catheter clamp is rigid plastic or metal; the material of the uterine cavity catheter clamp is rigid plastic or metal; and the material of the uterine cavity catheter part is PVC, TPE, PE or PP.

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