Rapid blood type detection reagent card

Abstract

The utility model discloses a blood type rapid detection reagent card. The blood type rapid detection reagent card comprises an upper cover, a base and a test strip, the upper cover covers the base, the upper cover and the base form a mounting cavity, and the upper cover is further provided with a sample adding hole and a flushing hole which are arranged at intervals; the test strip is arranged on the base and located in the mounting cavity, orthographic projections of the sample adding hole and the flushing hole on the base correspond to part of the test strip, the test strip comprises a quality control line and at least two detection lines, one detection line is coated with an A-type blood antibody, the other detection line is coated with a B-type blood antibody, and the quality control line is coated with an erythrocyte antibody. The orthographic projections of the sample adding hole and the flushing hole on the base correspond to part of the test strip, and multiple blood group antibodies are arranged on the same test strip, so that a detection result can be obtained only through one-time sample adding, the sample size required by detection is reduced, the whole operation steps are simple, the demand quantity and the whole structure of the test strip are reduced, and the cost is reduced. The production cost is reduced.

Description

TECHNICAL FIELD

[0001] The application relates to the technical field of biological detection, in particular to a blood type rapid detection reagent card. BACKGROUND

[0002] Blood type detection is an important prerequisite for ensuring safe blood transfusion, and also has important application value in ethnology, genetics, forensic science, transplantation immunity, disease resistance (or susceptibility) and the like. In clinical medicine, in addition to blood transfusion and transplantation immunity, the examination of specific antibodies of neonatal hemolytic disease and autoimmune hemolytic anemia also requires blood type knowledge and related technology.

[0003] The blood type detection method includes detection by using a blood type rapid detection reagent card. The blood type rapid detection reagent card on the market currently includes a sample adding hole and a washing hole. The sample adding hole is used for adding a detection blood sample, and the washing hole is used for dropping a washing liquid to wash the detection blood sample. The number of the sample adding holes is consistent with the number N of red blood cell surface antigen types. In specific operation, N times of sample adding and N times of washing liquid adding are required. Therefore, it can be known that the liquid adding is frequent when the detection is performed by using the above blood type rapid detection reagent card, and the operation steps are not simple enough. CONTENT OF THE INVENTION

[0004] Therefore, it is necessary to provide a blood type rapid detection reagent card with simple operation steps.

[0005] An embodiment of the application provides a blood type rapid detection reagent card.

[0006] A blood type rapid detection reagent card, characterized in that the blood type rapid detection reagent card comprises an upper cover, a base and a test strip.

[0007] The upper cover is combined with the base and forms a mounting cavity. The upper cover is further provided with a sample adding hole and a washing hole which are arranged at intervals.

[0008] The test strip is arranged in the base and located in the mounting cavity. The orthographic projections of the sample adding hole and the washing hole on the base correspond to part of the test strip. The test strip comprises a quality control line and at least two detection lines. One of the detection lines is coated with anti-A blood antibody, and the other detection line is coated with anti-B blood antibody. The quality control line is coated with anti-red blood cell antibody.

[0009] In some embodiments, the center of the sample adding hole and the center of the washing hole are located on the same straight line.

[0010] In some embodiments, the test strip comprises a base plate, a sample pad, a nitrocellulose membrane and a water absorption pad arranged in sequence on the base plate, the nitrocellulose membrane is provided with the detection line and the quality control line arranged at intervals, the sample hole is opposite to the nitrocellulose membrane, and the washing hole is opposite to the sample pad.

[0011] In some embodiments, the upper cover is further provided with an observation window, the observation window comprises a first window and a second window, the first window and the second window are distributed on both sides of the sample hole, the first window is opposite to the detection line, and the second window is opposite to the quality control line.

[0012] In some embodiments, the two detection lines are a first sub-detection line and a second sub-detection line, the first window and the second window are opposite to the second sub-detection line and the quality control line respectively, and the sample hole is opposite to the first sub-detection line.

[0013] In some embodiments, the blood rapid detection reagent card further comprises a limiting module, the limiting module comprises two limiting components arranged at intervals on the base, and the two limiting components are respectively used for fixing two ends of the test strip.

[0014] In some embodiments, the limiting component comprises an open-ended limiting enclosure and two first limiting strips, one end of the two first limiting strips is arranged at intervals in the limiting enclosure, and a first clamping space for clamping the test strip is formed between the two first limiting strips.

[0015] In some embodiments, the limiting module further comprises a reinforcing component between the two limiting components, the reinforcing component comprises two second limiting strips arranged at intervals, and a second clamping space for clamping the test strip is formed between the two second limiting strips.

[0016] In some embodiments, the blood rapid detection reagent card further comprises a locking component, the locking component comprises a first locking part and a second locking part, the first locking part is provided with a clamping hole, the second locking part is provided with a clamping column matched with the clamping hole, the first locking part is arranged on one of the base and / or the upper cover, and the second locking part is arranged on the other one.

[0017] In some embodiments, the first locking part comprises a locking column, the clamping hole is arranged in the height direction of the locking column, and the second locking part further comprises a plurality of limiting convex strips arranged at intervals in the circumferential direction of the clamping column.

[0018] The blood type rapid detection reagent card provided by the application has the advantages that the positive projection of the sample adding hole and the flushing hole on the base corresponds to the part of the test paper strip, and a plurality of blood type antibodies are arranged on the same test paper strip. One sample adding operation is performed to make the detection blood sample enter the test paper strip through the sample adding hole and diffuse on the test paper strip to the detection line and the quality control line. Then, one flushing liquid adding operation is performed to make the flushing liquid enter the test paper strip and flush the detection blood sample on the test paper strip, so that the color development of the detection line and the quality control line is obtained to determine the blood type. The whole operation procedure is simple, and the detection result can be obtained only by one sample adding operation, which reduces the sample amount required for detection, and the plurality of blood type antibodies arranged on the same test paper strip also helps to reduce the demand amount of the test paper strip and the overall structure, and reduce the production cost. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 A structural schematic diagram of a blood type rapid detection reagent card provided by the application is provided.

[0020] Figure 2 A structural schematic diagram of a test paper strip in the blood type rapid detection reagent card is provided. Figure 1

[0021] Figure 3 A structural schematic diagram of another blood type rapid detection reagent card provided by the application is provided.

[0022] Figure 4 A structural schematic diagram of a test paper strip in the blood type rapid detection reagent card is provided. Figure 3

[0023] Explanation of the reference signs:

[0024] 10, blood type rapid detection reagent card; 100, upper cover; 110, sample adding hole; 120, flushing hole; 130, observation window; 131, first window; 132, second window; 140, identification area;

[0025] 200, base;

[0026] 300, test paper strip; 310, bottom plate; 320, sample pad; 330, nitrocellulose membrane; 340, water absorption pad; 350, detection line; a, first detection line; b, second detection line; 360, quality control line;

[0027] 400, mounting cavity;

[0028] 500, limiting module; 510, limiting assembly; 511, limiting surrounding wall; 5111, first limiting groove; 5112, inclined wall; 512, first limiting strip; 513, first baffle; 5131, second limiting groove; 520, reinforcing assembly; 521, second limiting strip; 522, second baffle;

[0029] ​​600, locking assembly; 610, first locking member; 611, locking post; 612, clamping hole; 620, second locking member; 621, clamping post; 622, limiting convex strip. DETAILED DESCRIPTION

[0030] In order to make the above objectives, features and advantages of the present application more apparent, specific embodiments of the present application are described in detail below with reference to the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application. However, the present application can be practiced in a number of different ways beyond the specific details described herein. Thus, the present application is not intended to be limited to the embodiments described herein and illustrated in the drawings.

[0031] In the description of the present application, it should be understood that, if there are terms such as "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. appear, these terms indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the purpose of facilitating the description of the present application and simplifying the description, and therefore cannot be understood as indicating or implying that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application.

[0032] In addition, if the terms "first", "second" appear, these terms are only for the purpose of description, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features referred to. Therefore, the features limited by "first", "second" can explicitly or implicitly include at least one of the features. In the description of the present application, if the term "multiple" appears, the meaning of "multiple" is at least two, such as two, three, etc., unless otherwise explicitly specified and limited.

[0033] In the present application, unless otherwise explicitly specified and limited, if the terms "mounting", "connecting", "connecting", "fixing" and the like appear, these terms should be interpreted broadly. For example, it can be fixedly connected, or it can be detachably connected, or it can be integrated; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the internal communication of two elements or the interaction relationship between two elements, unless otherwise explicitly limited. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0034] In the present application, unless otherwise explicitly specified and limited, if there is a description such as "on" or "under" or the like between a first feature and a second feature, it can mean that the first and second features are in direct contact or the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature can be "above", "over" and "on" the second feature, which can mean that the first feature is directly above or obliquely above the second feature, or only means that the first feature is horizontally higher than the second feature. The first feature can be "below", "under" and "under" the second feature, which can mean that the first feature is directly below or obliquely below the second feature, or only means that the first feature is horizontally lower than the second feature.

[0035] It should be noted that if an element is referred to as being "fixed" or "disposed" on another element, it can be directly on the other element or there can be an intermediate element. If an element is considered to be "connected" to another element, it can be directly connected to the other element or there can be an intermediate element. If present, the terms "vertical", "horizontal", "up", "down", "left", "right" and the like used in the present application are only for illustrative purposes and do not represent the only implementation.

[0036] Referring to Figure 1 and Figure 2 shown, Figure 1 a structural schematic diagram of a blood type rapid detection reagent card 10 according to an embodiment of the present application, Figure 2 for Figure 1 a test strip 300 in the present application. The blood type rapid detection reagent card 10 provided by an embodiment of the present application includes an upper cover 100, a base 200 and a test strip 300, wherein the upper cover 100 is covered on the base 200 and the two form a mounting cavity 400, and the upper cover 100 is also provided with a sample adding hole 110 and a washing hole 120 arranged at intervals. In specific use, the sample adding hole 110 is used for adding a detection blood sample, and the washing hole 120 is used for adding a washing liquid to wash the detection blood sample.

[0037] The test strip 300 is arranged on the base 200 and located in the mounting cavity 400, the orthographic projection of the sample adding hole 110 and the flushing hole 120 on the base 200 corresponds to part of the test strip 300, the test strip 300 includes a quality control line 360 and at least two detection lines 350, one of which is coated with anti-A blood antibody, and the other is coated with anti-B blood antibody, and the quality control line 360 is coated with anti-red blood cell antibody. It should be noted that when the number of detection lines 350 is two and respectively coated with anti-A blood antibody and anti-B blood antibody, the above-mentioned blood type rapid detection reagent card 10 is used for ABO blood type identification. The basic principle is to use the specific binding of antigen and antibody and the red mark of red blood cells itself. When the detection blood sample contacts the antibody coated on the detection line 350, the specific antigen on the surface of the red blood cells expressing blood type and the antibody are combined, and the position of the detection line 350 coated with the corresponding blood type antibody appears a red band. All blood types of red blood cells will combine with the anti-red blood cell antibody coated on the quality control line 360, and a red band should appear. When the quality control line 360 does not develop color, it indicates that the detection is invalid and needs to be re-detected.

[0038] Through the above arrangement, when identifying the ABO blood type, the detection blood sample is added in the sample adding hole 110. When the quality control line 360 and the detection line 350 coated with anti-A blood antibody develop color, the detection blood sample is A blood; when the quality control line 360 and the detection line 350 coated with anti-B blood antibody develop color, the detection blood sample is B blood; when the quality control line 360, the detection line 350 coated with anti-A blood antibody and the detection line 350 coated with anti-B blood antibody all develop color, the detection blood sample is AB blood; and when the quality control line 360 develops color, the detection line 350 coated with anti-A blood antibody and the detection line 350 coated with anti-B blood antibody all do not develop color, the detection blood sample is O blood.

[0039] It should be emphasized that in addition to the most common ABO blood type, the second is the RH blood type system (identified according to whether there is D antigen on the red blood cells). When the RH blood type system identification needs to be performed, a detection line 350 coated with anti-D blood antibody is added to the test strip 300, and the identification principle of the RH blood type is similar to that of the ABO blood type, which will not be described in detail here.

[0040] The rapid blood type detection reagent card 10, by setting the positive projection corresponding part of the test paper strip 300 on the sample hole 110 and the washing hole 120 on the base 200, and the same test paper strip 300 is provided with a plurality of blood type antibodies, by adding sample in the sample hole 110 once, so that the detection blood sample enters the test paper strip 300 through the sample hole 110 and diffuses on the test paper strip 300 to the detection line 350 and the control line 360, and then by adding washing liquid in the washing hole 120 once, the washing liquid enters the test paper strip 300 and washes the detection blood sample on the test paper strip 300, to obtain the color development of the detection line 350 and the control line 360 to judge the blood type. The whole operation steps are simple, only one sample adding can obtain the detection result, reduces the sample amount required for detection, and the same test paper strip 300 is provided with a plurality of blood type antibodies, which is also beneficial to reduce the demand amount of the test paper strip 300 and the overall structure, and reduce the production cost.

[0041] In order to facilitate the washing liquid to wash the detection blood sample quickly to develop color quickly, in a preferred embodiment, the center of the sample hole 110 and the center of the washing hole 120 are located on the same straight line. In order to facilitate the washing liquid to enter the washing hole 120, when specifically setting, the size of the washing hole 120 gradually decreases from the side of the outer surface of the upper cover 100 to the side of the inner part of the upper cover 100, so as to add the washing liquid. It should be noted that in order to facilitate the user to quickly understand the product, the outer surface of the upper cover 100 is also provided with a mark area 140. The mark area 140 can be provided with a label, a company trademark and a one-dimensional code or a two-dimensional code of goods and the like.

[0042] In order to quickly complete the detection, in a preferred embodiment, the test paper strip 300 includes a bottom plate 310 and a sample pad 320, a nitrocellulose membrane 330 (NC membrane) and a water absorbing pad 340 arranged in sequence on the bottom plate 310, the nitrocellulose membrane 330 is provided with a detection line 350 and a control line 360 arranged at intervals, the sample hole 110 is directly opposite the nitrocellulose membrane 330, and the washing hole 120 is directly opposite the sample pad 320. Through the above setting, the detection blood sample entering the test paper strip 300 from the sample hole 110 will quickly diffuse to the detection line 350 and the control line 360 through the acid cellulose membrane, the washing liquid entering the test paper strip 300 from the washing hole 120 will be chromatographed to the sample at the detection line 350 and the control line of the NC membrane, and finally the excess washing liquid and the detection blood sample will be absorbed by the water absorbing pad 340 at the tail end, so as to complete the whole detection process.

[0043] In a specific arrangement, the bottom plate 310 is preferably a PVC plate, i.e., a polyvinyl chloride plate, which has the advantages of weather resistance, water and moisture resistance, corrosion resistance, light weight, easy processing, and high cost performance. The bottom plate 310 has a long strip structure, and the sample pad 320, the nitrocellulose membrane 330, and the water absorption pad 340 all extend along the length direction of the bottom plate 310. The sample pad 320 and the water absorption pad 340 are overlapped above the left and right ends of the nitrocellulose membrane 330.

[0044] To facilitate the user to observe the color development, specifically, the upper cover 100 is also provided with an observation window 130, which includes a first window 131 and a second window 132. The first window 131 and the second window 132 are distributed on both sides of the sample well 110. In a specific arrangement, the centers of the first window 131, the second window 132, and the sample well 110 are located on the same straight line. The first window 131 is directly opposite to all the detection lines 350, and the second window 132 is directly opposite to the quality control line 360. In a specific arrangement, the quality control line 360 is located at the middle position of the second window 132, and the two detection lines 350 are symmetrically arranged about the center point of the first window 131 and located at the middle position of the first window 131.

[0045] Referring to Figure 3 and Figure 4 , it is shown that Figure 3 is a structural schematic view of a blood type rapid detection reagent card 10 according to another embodiment of the present application, Figure 4 is a structural schematic view of a test strip 300 in Figure 3 . To more intuitively observe the color development result, more specifically, the two detection lines 350 are a first sub-detection line 350 and a second sub-detection line 350. In a specific arrangement, the first sub-detection line 350 is coated with one of an anti-A blood antibody or an anti-B blood antibody, and the second sub-detection line 350 is coated with the other of the above two. The first window 131 and the second window 132 are directly opposite to the second sub-detection line 350 and the quality control line 360, respectively, and the sample well 110 is directly opposite to the first sub-detection line 350. In a specific arrangement, the first window 131 and the second window 132 are symmetrically arranged about the sample well, and correspondingly, the second sub-detection line 350 and the quality control line 360 are symmetrically arranged about the first sub-detection line 350. The user can observe one color development condition through the sample well 110, the first window 131, and the second window 132, and more conveniently determine the blood type. The identification principle and process of the above-mentioned blood type rapid detection reagent card 10 are similar to those of the blood type rapid detection reagent card 10 with Figure 1 the structure, which will not be described in detail here.

[0046] Referring to Figure 1 and Figure 3As shown, in order to facilitate the fixation of the test strip 300, a preferred embodiment of the blood type rapid detection reagent card 10 further comprises a limiting module 500, which comprises two limiting assemblies 510 arranged at intervals on the base 200, and the two limiting assemblies 510 are respectively used for fixing two ends of the test strip 300. In order to more conveniently design the limiting assembly 510, specifically, the limiting assembly 510 comprises an open-ended limiting surrounding wall 511 and two first limiting strips 512, one end of the two first limiting strips 512 is arranged at intervals in the limiting surrounding wall 511, and a first clamping space for clamping the test strip 300 is formed between the two first limiting strips 512.

[0047] In a specific arrangement, the side of the two first limiting strips 512 away from each other is further provided with a first baffle 513, and the first baffle 513 is used for limiting the position of the test strip 300. The limiting surrounding wall 511 is preferably a U-shaped structure and has a certain height, and the limiting surrounding wall 511 can limit the end of the test strip 300 on one hand, and support the upper cover 100 on the other hand. One of the limiting surrounding walls 511 abuts against the inner wall of the washing hole 120, in order to adapt the shape of the limiting surrounding wall 511 to the inner wall of the washing hole 120, the top end of the limiting surrounding wall 511 is provided with an inclined wall 5112.

[0048] In order to be able to fix the middle section of the test strip 300, more specifically, the limiting module 500 further comprises a reinforcing assembly 520 located between the two limiting assemblies 510, and the reinforcing assembly 520 comprises two second limiting strips 521 arranged at intervals, and a second clamping space for clamping the test strip 300 is formed between the two second limiting strips 521. In a specific arrangement, the side of the two second limiting strips 521 away from each other is provided with a second baffle 522. The second baffle 522 can support the upper cover 100 while limiting the test strip 300.

[0049] In order to facilitate the detachable connection of the upper cover 100 and the base 200, a preferred embodiment, the blood type rapid detection reagent card 10 further comprises a locking assembly 600, the locking assembly 600 comprises a first locking member 610 and a second locking member 620, the first locking member 610 is provided with a clamping hole 612, the second locking member 620 is provided with a clamping column 621 matched with the clamping hole 612, the first locking member 610 is arranged on one of the base 200 and / or the upper cover 100, and the second locking member 620 is arranged on the other one. In a specific arrangement, the first locking member 610 comprises a locking column 611, the clamping hole 612 is formed in the height direction of the locking column 611, and the second locking member 620 further comprises a plurality of limiting convex strips 622, the plurality of limiting convex strips 622 are arranged at intervals in the circumferential direction of the clamping column 621. In a specific arrangement, the limiting convex strips 622 are distributed in a cross shape. Through the above arrangement, the upper cover 100 and the base 200 are embedded and matched with each other through the locking assembly 600, so as to realize the detachable connection of the shell base 200 and the upper cover 100.

[0050] The present application takes the first locking member 610 and the second locking member 620 arranged on the two opposite sides of the base 200 as an example for illustration, and the number of the first locking member 610 and the second locking member 620 is multiple, wherein two first locking members 610 and two second locking members 620 are arranged at four corner positions of the base 200. It should be noted that in order to strengthen the fixation of the upper cover 100 and the base 200, the inner side of the limiting surrounding wall 511 and the first baffle 513 are respectively provided with a first limiting recess 5111 and a second limiting recess 5131, and the upper cover 100 is provided with corresponding first clamping columns and second clamping columns.

[0051] The technical features of the above-mentioned embodiments can be combined in any way. In order to make the description simple, all possible combinations of the technical features in the above-mentioned embodiments are not described, but as long as the combinations of the technical features do not exist contradictory, they should be considered as the scope of the present application.

[0052] The above-mentioned embodiments only express several implementation ways of the present application, and the description is more specific and detailed, but it should not be understood as a limitation on the patent scope of the application. It should be noted that for ordinary skilled persons in the art, some modifications and improvements can be made without departing from the concept of the present application, and these belong to the protection scope of the present application. Therefore, the protection scope of the patent of the present application should be subject to the appended claims.

Claims

1. A rapid blood type testing kit, characterized in that, The rapid blood typing test kit includes a top cover, a base, and a test strip, wherein: The upper cover fits onto the base and the two form an installation cavity. The upper cover also has a sample addition hole and a rinsing hole that are spaced apart. The test strip is disposed on the base and located in the mounting cavity. The orthographic projections of the sample application hole and the rinsing hole on the base correspond to portions of the test strip. The test strip includes a control line and at least two detection lines, one of which is coated with anti-A blood antibodies, the other of which is coated with anti-B blood antibodies, and the control line is coated with anti-erythrocyte antibodies.

2. The rapid blood type testing reagent card according to claim 1, characterized in that, The center of the sample application hole and the center of the flushing hole are on the same straight line.

3. The rapid blood type testing reagent card according to claim 1, characterized in that, The test strip includes a base plate and a sample pad, a nitrocellulose membrane, and an absorbent pad arranged sequentially on the base plate. The nitrocellulose membrane has the detection line and the quality control line arranged at intervals. The sample application hole is directly opposite the nitrocellulose membrane, and the rinsing hole is directly opposite the sample pad.

4. The rapid blood type testing reagent card according to claim 3, characterized in that, The top cover is also provided with an observation window, which includes a first window and a second window. The first window and the second window are distributed on both sides of the sample application hole. The first window is directly opposite the detection line, and the second window is directly opposite the quality control line.

5. The rapid blood type testing reagent card according to claim 4, characterized in that, The two detection lines are a first sub-detection line and a second sub-detection line, the first window and the second window are respectively opposite to the second sub-detection line and the quality control line, and the sample application well is opposite to the first sub-detection line.

6. The rapid blood type testing reagent card according to claim 1, characterized in that, The rapid blood type test kit also includes a limiting module, which includes two limiting components spaced apart from the base. The two limiting components are used to fix the two ends of the test strip.

7. The rapid blood type testing reagent card according to claim 6, characterized in that, The limiting component includes a limiting wall with one end open and two first limiting strips. One end of each of the two first limiting strips is spaced apart inside the limiting wall, and a first clamping space for clamping the test strip is formed between the two first limiting strips.

8. The rapid blood type testing reagent card according to claim 7, characterized in that, The limiting module also includes a reinforcing component located between the two limiting components. The reinforcing component includes two spaced-apart second limiting strips, and a second clamping space for clamping the test strip is formed between the two second limiting strips.

9. The rapid blood type testing reagent card according to claim 1, characterized in that, The rapid blood typing test kit also includes a locking assembly, which includes a first locking accessory and a second locking accessory. The first locking accessory has a snap-fit ​​hole, and the second locking accessory has a snap-fit ​​post that is adapted to the snap-fit ​​hole. The first locking accessory is disposed on one of the base and / or the top cover, and the second locking accessory is disposed on the other of the two.

10. The rapid blood type testing reagent card according to claim 9, characterized in that, The first lock accessory includes a locking post, and the locking post has a snap-fit ​​hole along its height direction. The second lock accessory also includes a plurality of limiting protrusions, which are spaced apart circumferentially along the snap-fit ​​post.

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