Multi-joint detection chromatography card box with adjustable flow velocity

Abstract

The utility model discloses a multi-joint detection chromatography card box with adjustable flow velocity. The multi-joint detection chromatography card box comprises a chip bottom plate and a substrate, the lower end surface of the chip bottom plate is provided with at least more than one reaction area, liquid separation area and air holes; the sample adding hole is formed in the chip bottom plate, penetrates through the liquid separation area and is used for adding a sample into the liquid separation area; the chromatography strips are fixed in the corresponding reaction zones; a main flow channel and a tree-shaped flow channel connected with the main flow channel are formed in the liquid separation area, the sample adding hole is communicated with the main flow channel, and the reaction area is communicated with the tree-shaped flow channel. According to the utility model, the tree-shaped flow channel liquid separation technology is adopted, and the adaptation to different detection items is realized through a flow channel liquid separation mode. By means of the tree-shaped flow channels, the tedious operation of multiple times of sample adding can be effectively avoided, sample adding errors and cross contamination are reduced, meanwhile, the flow speed of each flow channel can be flexibly adjusted, it is ensured that each detection item can be conducted under the condition of the most appropriate flow speed, and therefore the detection accuracy, sensitivity and operation convenience are improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to micro -fluidic technology and chromatography detection technical field, concretely is a kind of multi-association inspection chromatography card box of adjustable flow rate. BACKGROUND

[0002] Current market chromatography card box is mostly single inspection design, usually only one detection item can be carried out each time.Although some multi-association inspection card boxes have been proposed, these multi-association inspection card boxes still face the trouble of needing to add sample multiple times, increase operation steps, easily introduce human error and pollution, seriously affect the accuracy and reliability of detection.In addition, in multi-association inspection platform, since each detection item can have different requirements for flow rate, traditional multi-association inspection platform is often difficult to realize flexible adjustment of the flow rate of multiple flow channels, thereby leading to detection conditions mismatch, unable to meet the specific needs of different detection items, affect detection effect.

[0003] Specifically, the prior art has the following problems:

[0004] 1. Sample adding is complicated and error-prone: although the existing multi-association inspection card box supports multiple detection items, it still usually needs the user to manually add sample between each detection step, which not only increases the operation complexity, but also easily leads to sample adding error, even causes cross contamination, affects the accuracy and repeatability of detection results.

[0005] 2. Flow rate adjustment is not flexible: for different detection items, their ideal flow rate conditions can differ greatly, however, the existing multi-association inspection card box system mostly adopts fixed flow rate or single flow channel flow rate design, it is difficult to flexibly adjust the flow rate according to different detection needs, leading to inaccurate fluid control during detection process, affecting reaction efficiency and result accuracy.

[0006] 3. Lack of precise liquid distribution control: traditional card boxes often cannot provide sufficient accuracy in splitting or liquid distribution operation, especially in the case of multiple detection items requiring different flow or flow rate, uneven splitting is easy to occur, thereby affecting accurate distribution of sample and detection results. INVENTION CONTENTS

[0007] The utility model aims at providing multi-association inspection chromatography card box of adjustable flow rate to solve the problems proposed in the above background art.

[0008] To achieve the above-mentioned purpose, the utility model provides the following technical scheme:

[0009] A multi-association inspection chromatography card box of adjustable flow rate, comprising: a chip bottom plate and a substrate.

[0010] The chip bottom plate and the substrate are bonded.

[0011] The lower end surface of the chip base plate has at least two reaction zones;

[0012] The lower end surface of the chip base plate is provided with a liquid distribution zone and air holes;

[0013] A sample adding hole is formed in the chip base plate and penetrates the liquid distribution zone, for adding sample to the liquid distribution zone;

[0014] The air holes are in communication with the reaction zones;

[0015] A chromatographic strip is placed in the reaction zone;

[0016] The liquid distribution zone is provided with a main flow channel and tree-shaped flow channels connected to the main flow channel, the sample adding hole is in communication with the main flow channel, and the reaction zone is in communication with the tree-shaped flow channels.

[0017] Further, the reaction zones are separated by a partition, and the outer end surface of the partition is flush with the lower end surface of the chip base plate.

[0018] Further, the chip base plate and the substrate are relatively fixed by a pre-positioning component.

[0019] Further, the pre-positioning component includes a column with specific elasticity and a positioning block, the column is installed at one end of the upper end surface of the substrate and is provided with an anti-disengagement part, the positioning block is installed at one end of the lower end surface of the chip base plate and is away from the column, the lower end surface of the chip base plate has a hole for the column and the anti-disengagement part to insert, and the upper end surface of the substrate has a slot for the positioning block to insert.

[0020] Further, the anti-disengagement part is in the shape of a circular truncated cone, the hole bottom of the hole has a depth greater than the length of the anti-disengagement part, and the hole bottom is used for the anti-disengagement part to move in the axial direction.

[0021] Further, the substrate is made of glass, and the glass is quartz glass or soda-lime glass, and the chip base plate is made of plastic.

[0022] Further, the size of the tree-shaped flow channels is between 50 microns and 500 microns.

[0023] Further, the sample adding hole is in the shape of a circle or a square rectangle and is in the shape of a funnel, for preventing sample from remaining on the sample adding hole.

[0024] Compared with the prior art, the chip base plate has the following beneficial effects:

[0025] 1. Simplify operation steps, reduce sampling errors and pollution: through the tree-shaped flow channel distribution technology, the sample can be automatically distributed to multiple flow channels into the reaction zone after one-time sampling, avoiding the cumbersome steps of multiple sampling, reducing the errors introduced by human operation and the risk of cross contamination. This design not only improves the efficiency of detection, but also significantly improves the processing accuracy of the sample and the reliability of the detection result.

[0026] 2. Precise flow rate adjustment, flexible adaptation to different detection requirements: the size of the tree-shaped flow channel is between 50 microns and 500 microns, which realizes independent regulation of the flow rate of each flow channel in the distribution area, so as to adjust the flow rate according to the specific requirements of different detection items. For example, some detection may require slower flow rate to increase reaction time, while other detection may require faster flow rate to improve detection speed. The traditional fixed flow rate design cannot do this, while the present scheme can accurately adapt to various detection requirements through flexible flow rate adjustment, ensuring the best detection conditions for each item.

[0027] 3. Improve system adaptability and diversity: through accurate distribution and flow rate regulation, the platform can support multiple detection items at the same time, and each detection item is optimized independently according to its own requirements. This flexibility enables the cartridge to be widely used in complex multi-detection scenarios, such as detecting different targets, diseases or substances at the same time, without worrying about flow rate mismatch or sampling error affecting the results.

[0028] 4. Improve detection throughput and efficiency: multiple detections can be completed at one time through the sampling hole, significantly improving the detection throughput. At the same time, since the flow rate can be adjusted, the time of fluid passing through the reaction area can be optimized according to the requirements of each item, thereby improving the efficiency and accuracy of detection and reducing the detection time.

[0029] 5. Reduce operation complexity and user burden: by simplifying the sampling process and providing precise flow rate control, the microfluidic platform of the present scheme can greatly reduce the operation difficulty, reduce human interference in the experiment process, reduce the burden of laboratory personnel, and improve the overall operation convenience.

[0030] In summary, the present utility model is a multi-detection chromatography cartridge based on a microfluidic platform, which adopts tree-shaped flow channel distribution technology to adapt to different detection items through flow channel distribution. Through the tree-shaped flow channel, the cumbersome operation of multiple sampling can be effectively avoided, the sampling error and cross contamination can be reduced, and the flow rate of each flow channel can be flexibly adjusted to ensure that each detection item can be performed under the most suitable flow rate conditions, thereby improving the accuracy, sensitivity and operation convenience of detection. BRIEF DESCRIPTION OF DRAWINGS

[0031] Figure 1 It is a structural schematic view of the embodiment 1 of the present utility model.

[0032] Figure 2 Figure 1 is a schematic view of the substrate and the chip bottom plate of the utility model embodiment 1.

[0033] Figure 3 Figure 2 is another perspective view schematic view of the utility model. Figure 2

[0034] Figure 4 Figure 3 is a schematic view of the utility model embodiment 2.

[0035] Figure 5 Figure 4 is another perspective view schematic view of the utility model. Figure 4

[0036] Figure 5 is a schematic view of the substrate and the chip bottom plate of the utility model embodiment 2 through the column body rotation certain angle. Figure 6 Figure: 1 - slot, 2 - chip bottom plate, 3 - substrate, 4 - sample hole, 5 - reaction area, 6 - liquid separation area, 7 - main flow channel, 8 - tree flow channel, 9 - air hole, 10 - chromatography strip, 11 - column body, 12 - anti-drop part, 13 - insertion hole, 14 - hole bottom, 15 - positioning block, 16 - blocking part.

[0037] DETAILED DESCRIPTION

[0038] The technical scheme in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model, apparently, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by the person skilled in the art without making creative labor belong to the protection scope of the utility model.

[0039] In the description of the utility model, it needs to be explained that the orientation or position relationship indicated by the terms "upper end", "lower end", "inner", "outer", "front end", "rear end", "two ends", "one end", "another end" and the like is the orientation or position relationship based on the orientation or position relationship shown in the drawings, only for the convenience of describing the utility model and simplifying the description, and not indicating or implying that the indicated device or element must have a particular orientation, a particular orientation and operation, therefore, it cannot be understood as the limitation of the utility model. In addition, the terms "first", "second" are only for the purpose of description, and cannot be understood as indicating or implying relative importance.

[0040] ​​In the description of the utility model, it is necessary to explain that, unless there are definite provisions and limitations, the terms "mount", "be provided with", "be provided with", "be provided with", "connect" and the like should be understood broadly, for example, "connect", can be fixedly connected, also can be detachably connected, or integrally connected;Can be mechanical connection, also can be electrical connection;Can be directly connected, also can be indirectly connected through intermediate medium, can be the communication inside two elements.For ordinary skilled in the art, the specific meaning of the above terms in the utility model can be understood according to specific circumstances.

[0041] Embodiment 1

[0042] Please refer to Figures 1 to 3 The utility model provides a technical scheme:

[0043] A multi-connection detection layer chromatography card box with adjustable flow rate, comprising: a chip bottom plate 2 and a substrate 3;

[0044] The chip bottom plate 2 and the substrate 3 are bonded;

[0045] The lower end face of the chip bottom plate 2 has at least two or more reaction zones 5;

[0046] The lower end face of the chip bottom plate 2 is provided with a liquid separation zone 6 and an air hole 9;

[0047] The chip bottom plate 2 is provided with a sample addition hole 4, which penetrates to the liquid separation zone 6, for adding sample to the liquid separation zone 6;

[0048] The air hole 9 is in communication with the reaction zone 5;

[0049] The reaction zone 5 is placed with a chromatography strip 10;

[0050] The liquid separation zone 6 is provided with a main flow channel 7 and a tree-shaped flow channel 8 connected with the main flow channel 7, the sample addition hole 4 is in communication with the main flow channel 7, and the reaction zone 5 is in communication with the tree-shaped flow channel 8, wherein the upper end face and the lower end face are based on the perspective. Figures 1 to 3

[0051] Specifically, the adjacent reaction zones 5 are separated by a blocking part 16, and the outer end face of the blocking part 16 is flush with the lower end face of the chip bottom plate 2.

[0052] Specifically, the substrate 3 can adopt glass materials such as quartz glass or soda lime glass, and the chip bottom plate 2 adopts plastic materials such as PMMA (acrylic) or PC (polycarbonate).

[0053] Specifically, the sample has the same path from the tree-shaped flow channel 8 to each reaction zone 5, and the size of the tree-shaped flow channel 8 is between 50 microns and 500 microns.

[0054] ​Specifically, the shape of the sample adding hole 4 is circular or square, and is funnel-shaped, which is used to prevent the sample from remaining on the sample adding hole 4.

[0055] The flow rate adjustable multi-detection chromatography card box of the embodiment is a multi-detection chromatography chip based on the chromatography principle. The flow rate adjustable multi-detection chromatography card box is mainly applied to the fields of biological analysis, environmental monitoring, clinical diagnosis, food safety detection and the like, and belongs to the cross fields of analytical chemistry, microfluidic chip technology and separation science. Specifically, the card box can be widely applied to multi-component simultaneous detection and analysis, especially in scenarios requiring high-throughput, rapid and accurate analysis, such as large-scale sample screening, qualitative and quantitative analysis of complex sample components and the like.

[0056] The flow rate adjustable multi-detection chromatography card box not only should have high separation performance, but also should have highly integrated design, so as to realize multi-component simultaneous separation, detection and quantitative analysis in the chip, so as to meet the application requirements of environmental protection, diversification, high efficiency and accuracy.

[0057] The material of the substrate 3 can be glass material (such as quartz glass or soda-lime glass and the like), which fully utilizes the natural hydrophilicity of glass, not only simplifies the liquid separation process, avoids the pollution and cost problems caused by the traditional hydrophilic membrane, but also improves the liquid separation precision, reduces the operation error, and significantly improves the performance, stability and economy of the multi-detection chip. Specifically, the card box of the glass material of the embodiment is bonded with the chip bottom plate 2 and the substrate 3 of the card box of the embodiment, and a plurality of reaction zones 5 are integrated in the card box, and the chromatography strip 10 is located in the reaction zone 5, thereby constituting an independent detection module, which can realize efficient detection of different components, and provides a new solution for solving the multi-component analysis bottleneck in the prior art.

[0058] In the embodiment, the chip bottom plate 2 is provided with a sample adding hole 4, a liquid separation zone 6 (a region enclosed by a dashed line), and a reaction zone 5 and an air hole 9. Figure 3

[0059] The shape of the sample adding hole 4 can be circular or square, and is funnel-shaped, which prevents the sample from remaining.

[0060] The liquid separation zone 6 and the sample adding hole 4 are separated by the tree-shaped flow channel 8, and the size of the tree-shaped flow channel 8 is between 50 microns and 500 microns.

[0061] By adjusting the size of the tree-shaped flow channel 8 to control the flow resistance, the liquid flow rate of the chip can be controlled. Specifically, the tree-shaped flow channel 8 has a plurality of branch flow channels (such as Figure 3 ​The three branch flow channels are designed to meet the independent regulation of the flow rate of each branch flow channel, so that the flow rate into the reaction zone 5 can be adjusted according to the specific requirements of different detection items.

[0062] The chromatographic strip 10 is placed in the reaction zone 5, and the reaction zone 5 is not limited to three-channel three-union detection, and can be further divided into four-union detection, five-union detection, etc.

[0063] Chip (i.e. the cartridge of the embodiment) assembly: place the processed chromatographic strip 10 into the reaction zone 5, and then use glue or double-sided tape to bond the chip bottom plate 2 and the substrate 3.

[0064] Specific detection process:

[0065] 1. Take 200 microliters of sample and add it to the sample well 4.

[0066] 2. The sample flows through the separation zone 6 into the chromatographic strip 10 in the reaction zone 5.

[0067] 3. The sample continues to flow forward on the chromatographic strip 10 by capillary force.

[0068] 4. After 10 minutes of reaction, observe the color development of the T line and the C line of the chromatographic strip 10. If only the C line is positive, if both the T and C lines are positive, and if neither the C line nor the T line is positive, the chromatographic strip 10 is invalid.

[0069] 5. Multi-union detection relies on the reagents coated on the chromatographic strip 10, and different reagents can be used to achieve multi-union detection.

[0070] Example 2

[0071] Please refer to Figures 4 to 6 The utility model provides a technical scheme, which adds the following content on the basis of example 1:

[0072] The chip bottom plate 2 and the substrate 3 are relatively fixed by a predetermined positioning component.

[0073] The predetermined positioning component includes a column 11 with specific elasticity and a positioning block 15, and the material is medical silica gel, silica gel or PE, etc., the column 11 is installed at one end of the upper end surface of the substrate 3, and a anti-loosening part 12 is installed, the positioning block 15 is installed at one end of the lower end surface of the chip bottom plate 2 and away from the column 11, the lower end surface of the chip bottom plate 2 has a insertion hole 13 for the column 11 and the anti-loosening part 12, and the upper end surface of the substrate 3 has an insertion slot 1 for the positioning block 15.

[0074] The anti-loosening part 12 is in the shape of a circular truncated cone, the depth of the hole bottom 14 of the insertion hole 13 is greater than the length of the anti-loosening part 12, and the hole bottom 14 is used for the anti-loosening part 12 to move in the axial direction.

[0075] In this embodiment, when bonding the chip base plate 2 and the substrate 3 with glue or double-sided tape, the four edges of the chip base plate 2 and the substrate 3 need to be aligned and covered together, and the alignment of the four edges needs to be careful and careful, and if there is a little vibration after alignment, the chip base plate 2 and the substrate 3 may be misaligned again, so the pre-positioning part is used, such as Figure 6 As shown, the chip base plate 2 and the substrate 3 are pre-fixed at one end, the chip base plate 2 and the substrate 3 are opened at a certain angle through the column 12, then the treated chromatographic strip 10 is placed in the flow channel of the reaction area 5, the glue or double-sided tape is coated or pasted on the chip base plate 2 or the substrate 3, and finally the chip base plate 2 and the substrate 3 are overlapped by rotating (the substrate 3 is rotated relative to the chip base plate 2 in the direction of the arrow A), and the positioning block 15 is inserted into the slot 1, which indicates that the four edges of the chip base plate 2 and the substrate 3 are completely aligned, thereby realizing the alignment and covering, and realizing the bonding of the chip base plate 2 and the substrate 3.

[0076] As shown in Figure 4 In this embodiment, since the maximum diameter of the anti-extraction part 12 is slightly larger than the diameter of the column 11, and the anti-extraction part 12 has elasticity, the anti-extraction part 12 can be inserted into the insertion hole 13 and finally into the hole bottom 14, and the diameter of the hole bottom 14 is the same as the maximum diameter of the anti-extraction part 12. The anti-extraction part 12 can move a certain distance along the axial direction with the hole bottom 14, so that the anti-extraction part 12 will not be separated from the hole bottom 14 under the condition that the chip base plate 2 and the substrate 3 are not pulled by a very large external force. Since the anti-extraction part 12 is inserted into the hole bottom 14, the pre-fixing of the chip base plate 2 and the substrate 3 can be realized.

[0077] When the chip base plate 2 and the substrate 3 are pre-fixed, the four edges of the chip base plate 2 and the substrate 3 are not necessarily completely aligned, that is, there is a certain angle between the chip base plate 2 and the substrate 3. At this time, the chip base plate 2 and the substrate 3 can be relatively rotated, and since the positioning block 15 has a certain height and protrudes outward from the lower end surface of the chip base plate 2, the depth of the hole bottom 14 is greater than the length of the anti-extraction part 12 and the column 11 also has elasticity, so that the chip base plate 2 or the substrate 3 can be slightly bent outward, and this embodiment takes the substrate 3 as an example to illustrate, that is, the substrate 3 is slightly lifted along the direction of the arrow B, so that when the substrate 3 is rotated relative to the chip base plate 2, the positioning block 15 on the chip base plate 2 can be easily inserted into the slot 1. Figure 6

[0078] The part not described in the utility model is the existing or known technology.

[0079] ​Although the embodiments of the present application have been shown and described, it is to be understood that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present application, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A multi-stage chromatography cartridge with adjustable flow rate, characterized in that, include: Chip base plate (2) and substrate (3); The chip substrate (2) and the base plate (3) are bonded together; The lower end face of the chip substrate (2) has at least two or more reaction zones (5); The lower end face of the chip substrate (2) is provided with a liquid separation area (6) and air holes (9); The chip substrate (2) is provided with a sample feeding hole (4), which extends into the liquid separation area (6) for adding samples to the liquid separation area (6); The pores (9) are connected to the reaction zone (5); Chromatographic strips (10) are placed in the reaction zone (5); The separation zone (6) is provided with a main channel (7) and a tree-shaped flow channel (8) connected to the main channel (7). The sample addition hole (4) is connected to the main channel (7), and the reaction zone (5) is connected to the tree-shaped flow channel (8).

2. The flow rate adjustable multi-stage chromatography cartridge as described in claim 1, characterized in that, Adjacent reaction zones (5) are separated by a partition (16), the outer end face of which is flush with the lower end face of the chip substrate (2).

3. The flow rate adjustable multi-stage chromatography cartridge as described in claim 1, characterized in that, The chip base plate (2) and the substrate (3) are fixed relative to each other by a pre-positioning component.

4. The flow rate adjustable multi-stage chromatography cartridge as described in claim 3, characterized in that, The pre-positioning component includes a specific elastic column (11) and a positioning block (15). The column (11) is installed on one end of the upper surface of the substrate (3) and is equipped with an anti-detachment part (12). The positioning block (15) is installed on one end of the lower surface of the chip base plate (2) and away from the column (11). The lower surface of the chip base plate (2) has an insertion hole (13) for inserting the column (11) and the anti-detachment part (12). The upper surface of the substrate (3) has a slot (1) for inserting the positioning block (15).

5. A multi-stage chromatography cartridge with adjustable flow rate as described in claim 4, characterized in that, The anti-detachment part (12) is frustum shaped, and the bottom (14) of the insertion hole (13) is deeper than the length of the anti-detachment part (12). The bottom (14) is used to allow the anti-detachment part (12) to move in the axial direction.

6. The flow rate adjustable multi-stage chromatography cartridge as described in claim 1, characterized in that, The substrate (3) is made of glass, which is either quartz glass or soda-lime glass, and the chip base plate (2) is made of plastic.

7. The flow rate adjustable multi-stage chromatography cartridge as described in claim 1, characterized in that, The size of the tree-like flow channel (8) is between 50 micrometers and 500 micrometers.

8. The flow rate adjustable multi-stage chromatography cartridge as described in claim 1, characterized in that, The sample application well (4) is circular or rectangular in shape, and is funnel-shaped, to prevent sample residue from remaining on the sample application well (4).

Images