Rapid detection kit for field harmful algae
By designing a rapid detection kit for harmful algae pre-loaded with reagents, the problems of inconvenience in carrying and cumbersome operation for detecting harmful algae in the field have been solved, achieving rapid and accurate on-site detection results.
Patent Information
- Application Number
- CN202422985716.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-04
- Publication Date
- 2025-11-25
- Estimated Expiration
- 2034-12-04
AI Technical Summary
In existing technologies, the detection of harmful algae in the wild is inconvenient to carry, cumbersome to operate, and easily affected by air pollution, which leads to reduced detection accuracy. It is especially difficult to carry out smoothly when there is a lack of professional personnel.
A rapid detection kit for harmful algae in the field has been designed, comprising a box, a lid, a reagent rack, and multiple harmful algae detection tubes. Each detection tube is pre-filled with RPA amplification reaction reagents, CRISPR reaction reagents, and a buffer solution. The design of the telescopic component enables automatic mixing and detection, avoiding the need to carry additional reagents and buffer solutions, and ensuring sealed operation.
It enables rapid and convenient detection of harmful algae in the field, avoids contamination caused by operational errors, and ensures the accuracy and airtightness of the detection. It is suitable for on-site detection in specific areas or when there are no professional personnel.
Smart Images
Figure CN223592728U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to the field of algal detection technology, especially to a kind of field harmful algal rapid detection kit. BACKGROUND
[0002] Harmful algae such as blue-green algae, golden algae, dinoflagellates and euglena have great harm to water resources, and the demand for on-site detection is increasing. Although traditional PCR-based molecular detection technology performs well in accuracy, it is difficult to set up and apply outside the laboratory due to the need for precise temperature control and supporting equipment, so it is limited in on-site detection applications.
[0003] RPA (recombinase polymerase amplification) as an isothermal nucleic acid amplification technology has great potential in POCT. RPA technology has many advantages such as low required temperature, strong amplification capacity, simple system and high sample tolerance, which makes it very suitable for developing POCT molecular detection tools. Based on this, POTC and RPA-CRISPR / Cas 12a-LFD technology are combined, and RPA-CRISPR / Cas 12a-LFD technology is developed. It first amplifies the collected sample by RPA technology, then uses Cas12a protein in CRISPR reagent to form a ternary complex with target DNA produced by RPA under the guidance of crRNA, and performs specific recognition. Once the target DNA is detected, the transverse cutting activity of Cas12a will be activated, then combined with lateral flow test strips, and the visual and non-specific ssDNA cutting activity of CRISPR / Cas 12a makes the detection result visualized in just 5 minutes.
[0004] However, there is currently no rapid detection kit for field harmful algae. Generally, reaction reagents and buffer solutions are carried and mixed with collected algal samples for on-site detection, which has the problems of inconvenience and complicated operation, air introduction during detection, pollution and reduction of detection accuracy, and is not convenient for the smooth development of detection work in specific areas or without professional personnel. UTILITY MODEL CONTENT
[0005] In view of the above-mentioned shortcomings of the prior art, the purpose of the utility model is to provide a field harmful algal rapid detection kit to facilitate the smooth development of detection work in specific areas or without professional personnel.
[0006] A field harmful algal rapid detection kit, comprising a box body and a box cover; the kit further comprises:
[0007] a reagent rack fixedly installed in the box body; and
[0008] a plurality of harmful algae detection tubes located in the box body;
[0009] Each of the harmful algae detection tubes is composed of a top cover, four tube bodies, an extension part I, an extension part II, and an extension part III, and a sleeve on the harmful algae detection tube is provided with three limiting strips, which respectively act on the extension part I, the extension part II, and the extension part III.
[0010] A first reaction assembly, a second reaction assembly, a buffer assembly, and a flow guide assembly are sequentially fixed in each of the harmful algae detection tubes from top to bottom, and an LFD test strip is arranged on the inner wall of the harmful algae detection tube; wherein the first reaction assembly contains RPA amplification reaction reagents, the second reaction assembly contains CRISPR reaction reagents, and the buffer assembly contains a buffer solution.
[0011] As a further improvement of the above scheme, the first reaction assembly is composed of a first reaction tube and a connecting ring; the buffer assembly and the second reaction assembly have the same structure, and the second reaction assembly includes a second reaction tube, a connecting tube connected to the second reaction tube, a first sharp fixedly installed at the top of the connecting tube, and a first flow guide cover; the flow guide assembly includes a flow guide tube, a second sharp, and a second flow guide cover.
[0012] As a further improvement of the above scheme, the first sharp installed on the second reaction assembly is in abutment with the first reaction tube, the first sharp installed on the buffer assembly is in abutment with the second reaction tube installed on the second reaction assembly, the second sharp is in abutment with the second reaction tube installed on the buffer assembly, and the liquid outlet end of the flow guide tube is in contact with the LFD test strip.
[0013] As a further improvement of the above scheme, the four tube bodies are arranged in parallel from top to bottom, and the extension part I is fixedly connected between the top tube body and the tube body adjacent thereto, the extension part II is fixedly connected between the two middle tube bodies, and the extension part III is fixedly connected between the bottom tube body and the tube body adjacent thereto.
[0014] As a further improvement of the above scheme, the extension part I, the extension part II, and the extension part III are all plastic bellows, the extension part I, the extension part II, and the extension part III have the same size, and the diameter of the extension part I is smaller than that of the tube body.
[0015] As a further improvement of the above scheme, the first reaction assembly, the second reaction assembly, the buffer assembly, and the flow guide assembly are respectively located in the four tube bodies, and the LFD test strip is vertically arranged in the bottom tube body; the connecting ring is fixedly installed between the outer edge of the first reaction tube and the corresponding tube body.
[0016] As a further improvement to the above solution, the first flow guide is fixedly sleeved at the liquid inlet end of the connecting pipe, and the first spike is located inside the first flow guide; a first connecting rod is fixed between the outer edge of the connecting pipe and the corresponding pipe body.
[0017] As a further improvement to the above solution, the second spike is fixedly installed at the top of the guide tube, and the second guide shroud is fixedly sleeved at the liquid inlet end of the guide tube, with the second spike located inside the second guide shroud.
[0018] As a further improvement to the above solution, a second connecting rod is fixed between the outer edge of the guide tube and the corresponding tube body.
[0019] As a further improvement to the above solution, the lid is hinged to the box body, and the opening and closing angle of the lid is from zero to 180 degrees; a latch is provided between the box body and the lid.
[0020] As a further improvement to the above solution, the longitudinal cross-section of the reagent rack is stepped, and several tube slots are evenly provided on the reagent rack for accommodating sample collection tubes; the harmful algae detection tube is located on the corresponding side of the reagent rack, and the harmful algae detection tube and the reagent rack are separated by a partition, which is fixed to the box body.
[0021] Compared with the prior art, the beneficial effects of this utility model are:
[0022] When used on-site, simply add the sample to be tested into the device; there is no need to bring additional reagents and buffer solutions for mixing, thus achieving rapid on-site testing. This facilitates the smooth conduct of testing in specific areas or where there are no professional personnel. The testing process is simple and easy to operate.
[0023] This avoids the possibility of the reaction tube being punctured due to operational errors or accidental contact, ensuring the accuracy of the detection. Furthermore, the entire detection process is conducted in a sealed manner, preventing air from entering and causing contamination, thus facilitating the detection of harmful algae in the field. Attached Figure Description
[0024] Figure 1 The diagram shown is a structural schematic of a rapid detection kit for harmful algae in the field provided by this utility model.
[0025] Figure 2 As shown Figure 1 A longitudinal section diagram.
[0026] Figure 3 As shown Figure 1 Enlarged view of the harmful algae detection tube.
[0027] Figure 4 As shown Figure 3 A sectional view.
[0028] Figure 5 shown is Figure 3 a structure diagram of a harmful algal detection tube.
[0029] Figure 6 shown is Figure 4 a structure diagram of a first reaction assembly.
[0030] Figure 7 shown is Figure 4 a structure diagram of a second reaction assembly.
[0031] Figure 8 shown is Figure 4 a structure diagram of a flow guide assembly.
[0032] Main element symbol explanation
[0033] 1, harmful algal detection tube; 101, tube body; 102, top cover; 103, expansion part I; 104, expansion part II; 105, expansion part III; 2, limiting strip; 3, first reaction assembly; 301, first reaction tube; 302, connecting ring; 4, second reaction assembly; 401, connecting tube; 402, first thorn; 403, first flow guide cover; 404, first connecting rod; 405, second reaction tube; 5, buffer assembly; 6, flow guide assembly; 601, flow guide tube; 602, second thorn; 603, second flow guide cover; 604, second connecting rod; 7, LFD test strip; 8, box body; 9, box cover; 10, reagent rack.
[0034] The above main element symbol explanation is further explained in detail in combination with the drawings and the specific embodiments. Specific embodiments
[0035] In order to make the purpose, technical scheme and advantages of the utility model more clear and obvious, the following will be combined with examples to further explain the utility model. The additional aspects and advantages of the utility model will be partly given in the following description, partly will become obvious from the following description, or will be understood through the practice of the utility model. It should be understood that the following description is only used to explain the utility model, and is not used to limit the utility model.
[0036] The specific embodiments of the utility model will be explained in detail below.
[0037] Please refer to Figures 1-2 The embodiment provides a field harmful algal rapid detection kit, which comprises a box body 8, a box cover 9, a reagent rack 10 fixedly installed in the box body 8 and a plurality of harmful algal detection tubes 1 in the box body 8.
[0038] The box cover 9 is hingedly connected to the box body 8, and the opening and closing angle of the box cover 9 ranges from zero to one hundred and eighty degrees. A lock catch is arranged between the box body 8 and the box cover 9. When the box cover 9 is closed, the lock catch locks the box body 8 and the box cover 9, and at this time, the reagent box is in a closed state for easy transfer. The reagent rack 10 is uniformly provided with a plurality of tube grooves for accommodating sample collection tubes. The longitudinal cross-sectional shape of the reagent rack 10 is a stepped shape. The stepped structure design allows multiple sample collection tubes to be placed reasonably, ensuring stability during transportation and storage. It should be noted that the inside of the box body 8 of the embodiment is provided with a foam board (not shown) for heat insulation, which also plays a role in buffering and shock absorption to some extent.
[0039] The harmful algae detection tube 1 is located at a corresponding position of the reagent rack 10, and the harmful algae detection tube 1 and the reagent rack 10 are separated by a partition plate fixed to the box body 8. Please continue to refer to Figures 3-8 , the harmful algae detection tube 1 is composed of a top cover 102, four tube bodies 101, an extension part I 103, an extension part II 104 and an extension part III 105. The four tube bodies 101 are arranged in parallel from top to bottom, and the extension part I 103 is fixedly connected between the top tube body 101 and the adjacent tube body 101. The extension part II 104 is fixedly connected between the two middle tube bodies 101, and the extension part III 105 is fixedly connected between the bottom tube body 101 and the adjacent tube body 101.
[0040] The extension part I 103, the extension part II 104 and the extension part III 105 are all plastic bellows. The extension part I 103, the extension part II 104 and the extension part III 105 are the same size, and the diameter of the extension part I 103 is smaller than that of the tube body 101. Three limiting strips 2 are arranged on the harmful algae detection tube 1, and the three limiting strips 2 act on the extension part I 103, the extension part II 104 and the extension part III 105, respectively.
[0041] The first reaction assembly 3, the second reaction assembly 4, the buffer assembly 5 and the flow guide assembly 6 are sequentially fixed in the harmful algae detection tube 1 from top to bottom, and the LFD test strip 7 is arranged on the inner wall of the harmful algae detection tube 1. In the harmful algae detection tube 1 of the embodiment, the first reaction assembly 3 is preloaded with RPA amplification reagents, the second reaction assembly 4 is preloaded with CRISPR reagents, and the buffer assembly 5 is preloaded with buffer solution. Therefore, when operating, the harmful algae collected in the field only needs to be added to the harmful algae detection tube 1, without the need to carry additional reagents and buffer solution for mixing, thereby achieving the effect of rapid on-site detection.
[0042] The first reaction component 3, the second reaction component 4, the buffer component 5 and the flow guide component 6 are respectively located in the four pipe bodies 101, and the first reaction component 3 is composed of a first reaction pipe 301 and a connecting ring 302. The connecting ring 302 is fixedly installed between the outer edge of the first reaction pipe 301 and the corresponding pipe body 101.
[0043] The buffer component 5 and the second reaction component 4 are the same in structure, and the second reaction component 4 comprises a second reaction pipe 405, a connecting pipe 401 connected with the second reaction pipe 405, a first sharp 402 fixedly installed at the top of the connecting pipe 401 and a first flow guide cover 403. The first flow guide cover 403 is fixedly sleeved at the liquid inlet end of the connecting pipe 401, and the first sharp 402 is located in the first flow guide cover 403. A first connecting rod 404 is fixed between the outer edge of the connecting pipe 401 and the corresponding pipe body 101.
[0044] The first sharp 402 installed on the second reaction component 4 is in abutment with the first reaction pipe 301, and the first sharp 402 installed on the buffer component 5 is in abutment with the second reaction pipe 405 installed on the second reaction component 4.
[0045] The flow guide component 6 comprises a flow guide pipe 601, a second sharp 602 and a second flow guide cover 603. The second sharp 602 is fixedly installed at the top of the flow guide pipe 601, and the second flow guide cover 603 is fixedly sleeved at the liquid inlet end of the flow guide pipe 601. The second sharp 602 is located in the second flow guide cover 603. A second connecting rod 604 is fixed between the outer edge of the flow guide pipe 601 and the corresponding pipe body 101. The LFD test strip 7 is vertically arranged in the bottom pipe body 101. The liquid outlet end of the flow guide pipe 601 is in contact with the LFD test strip 7, and the second sharp 602 is in abutment with the second reaction pipe 405 installed on the buffer component 5.
[0046] The use steps of the rapid detection kit of the embodiment are as follows:
[0047] S1 open the box cover 9, and take out a sample collection pipe and a harmful algae detection pipe 1 from the box body 8 respectively;
[0048] S2 operate the sample collection pipe to obtain a harmful algae sample to be detected;
[0049] S3 open the top cover 102, add the harmful algae sample into the first reaction pipe 301, then close the top cover 102, and react the sample to be detected with the RPA amplification reaction reagent in the first reaction pipe 301;
[0050] After the reaction in S4 is completed, the limiting strip 2 in the telescopic part I103 is removed, and then the two ends of the top tube body 101 and the tube body 101 adjacent thereto are pushed towards each other, so that the telescopic part I103 is contracted. At this time, the first sharp 402 arranged on the second reaction assembly 4 pierces the first reaction tube 301, and the reagent in the first reaction tube 301 after the reaction is completed flows into the second reaction tube 405 along the first flow guide cover 403 and the connecting tube 401, and reacts with the CRISPR reaction reagent in the second reaction tube 405;
[0051] After the reaction in S5 is completed, the limiting strip 2 in the telescopic part II104 is removed, and then the two ends of the tube body 101 are pushed towards each other, so that the telescopic part II104 is contracted. At this time, the first sharp 402 on the buffer assembly 5 pierces the second reaction tube 405 on the second reaction assembly 4, and the reagent after the reaction flows into the second reaction tube 405 containing the buffer solution, so that the solution after the reaction is mixed with the buffer solution.
[0052] After the reaction in S5 is completed, the limiting strip 2 in the telescopic part II104 is removed, and then the two ends of the tube body 101 are pushed towards each other, so that the telescopic part II104 is contracted. At this time, the first sharp 402 on the buffer assembly 5 pierces the second reaction tube 405 on the second reaction assembly 4, and the reagent after the reaction flows into the second reaction tube 405 containing the buffer solution, so that the solution after the reaction is mixed with the buffer solution.
[0053] The harmful algae detection tube 1 of the embodiment is made of transparent plastic material, so that the entire detection process can be visualized and the entire detection process and the final result can be directly observed. It should be noted that, during use, as long as the limiting strip 2 is not actively removed from the telescopic part I103, the telescopic part II104 and the telescopic part III105, the stability between the adjacent two tube bodies 101 can be ensured, and the reaction tube is prevented from being pierced in case of operation error or accidental touch, so that the reagent after incomplete reaction is prevented from flowing out of the pierced reaction tube, and the detection accuracy is affected. In addition, the rapid detection in the field is facilitated, air is prevented from entering to cause pollution, and the detection of harmful algae in the field is facilitated.
[0054] In summary, the rapid detection kit of the embodiment has the following advantages: the sample to be detected only needs to be added into the device, and no additional reagent and buffer solution need to be carried for mixing, so that the effect of rapid on-site detection is achieved. In addition, the reaction tube is prevented from being pierced in case of operation error or accidental touch, the detection accuracy is ensured, the entire detection process is sealed, air is prevented from entering to cause pollution, and the detection of harmful algae in the field is facilitated.
[0055] The preferred embodiments disclosed above are only used to help describe the utility model. The preferred embodiments do not describe all the details and do not limit the utility model to the specific embodiments. Obviously, according to the content of the specification, many modifications and changes can be made. The specification selects and specifically describes these embodiments in order to better explain the principle and practical application of the utility model, so that the person skilled in the art can well understand and utilize the utility model. The utility model is limited by the claims and the whole scope and equivalents thereof.
Claims
1. A rapid detection kit for harmful algae in the wild, comprising a kit body and a lid; Its features are, The kit also includes: The reagent rack is fixedly installed inside the box; and Several harmful algae detection tubes are located inside the box; Each of the harmful algae detection tubes consists of a top cover, four tube sections, telescopic part I, telescopic part II, and telescopic part III, and three limiting strips are provided on the harmful algae detection tubes, with the three limiting strips acting on telescopic part I, telescopic part II, and telescopic part III respectively. Each of the harmful algae detection tubes is fixed from top to bottom with a first reaction component, a second reaction component, a buffer component, and a flow guiding component, and an LFD test strip is attached to the wall of the harmful algae detection tube; wherein, the first reaction component contains RPA amplification reaction reagent, the second reaction component contains CRISPR reaction reagent, and the buffer component contains buffer solution.
2. The rapid test kit according to claim 1, characterized in that, The first reaction assembly consists of a first reaction tube and a connecting ring; The buffer assembly and the second reaction assembly have the same structure, and the second reaction assembly includes a second reaction tube, a connecting tube communicating with the second reaction tube, a first spike fixedly installed at the top of the connecting tube, and a first flow guide; the flow guide assembly includes a flow guide tube, a second spike, and a second flow guide. The first spike mounted on the second reaction assembly abuts against the first reaction tube, the first spike mounted on the buffer assembly abuts against the second reaction tube mounted on the second reaction assembly, the second spike abuts against the second reaction tube mounted on the buffer assembly, and the liquid outlet end of the guide tube contacts the LFD test strip.
3. The rapid test kit according to claim 2, characterized in that, The four pipe sections are arranged in parallel from top to bottom, and the telescopic part I is fixedly connected between the top pipe section and the adjacent pipe section, while the telescopic part II is fixedly connected between the two middle pipe sections. The telescopic part III is fixedly connected between the bottom tube and the adjacent tube.
4. The rapid test kit according to claim 3, characterized in that, The telescopic parts I, II and III are all plastic corrugated pipes. The telescopic parts I, II and III are the same size, and the diameter of the telescopic part I is smaller than that of the pipe body.
5. The rapid test kit according to claim 4, characterized in that, The first reaction component, the second reaction component, the buffer component, and the flow guiding component are respectively located in the four sections of the tube, and the LFD test strip is vertically arranged in the bottom tube. The connecting ring is fixedly installed between the outer edge of the first reaction tube and the corresponding tube body.
6. The rapid test kit according to claim 5, characterized in that, The first flow guide is fixedly sleeved at the liquid inlet end of the connecting pipe, and the first spike is located inside the first flow guide; A first connecting rod is fixed between the outer edge of the connecting pipe and the corresponding pipe body.
7. The rapid test kit according to claim 6, characterized in that, The second spike is fixedly installed at the top of the guide tube, and the second guide shroud is fixedly sleeved at the liquid inlet end of the guide tube, with the second spike located inside the second guide shroud.
8. The rapid test kit according to claim 7, characterized in that, A second connecting rod is fixed between the outer edge of the guide tube and the corresponding tube body.
9. The rapid test kit according to claim 1, characterized in that, The lid is hinged to the box body, and the opening and closing angle of the lid is from zero to one hundred and eighty degrees. A latch is provided between the box body and the box lid.
10. The rapid test kit according to claim 9, characterized in that, The reagent rack has a stepped cross-section and several tube slots are evenly distributed on it for holding sample collection tubes. The harmful algae detection tube is located on the corresponding side of the reagent rack, and the harmful algae detection tube and the reagent rack are separated by a partition, which is fixed to the box body.