Rat and mouse embryo transplantation device

Abstract

The utility model discloses a rat and mouse embryo transplantation device, and relates to the field of laboratory mouse operation instruments, the rat and mouse embryo transplantation device comprises an injector shell, a connector is integrally formed at the end part of the injector shell, an embryo suction tube is in interference connection with the connector, and a coarse adjustment piston is slidably arranged in the injector shell; a sealing ring is integrally formed on the outer side of the coarse adjustment piston, and the coarse adjustment piston is connected with a coarse adjustment pipe used for moving the coarse adjustment piston. A fine adjustment piston is arranged on the inner side of the coarse adjustment pipe in a sliding mode, and a fine adjustment rod used for moving the fine adjustment piston is connected to the fine adjustment piston. According to the device, the position of the coarse adjustment piston can be adjusted through the coarse adjustment pipe, so that the embryo suction pipe changes greatly, coarse adjustment is carried out when an embryo is sucked or pushed out, the position of the fine adjustment piston is adjusted through the fine adjustment rod, so that the embryo suction pipe changes slightly, small-amplitude adjustment is more convenient, and the operation difficulty is reduced.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of experimental mouse surgical equipment, in particular to a mouse embryo transfer device. BACKGROUND

[0002] Rats and mice are the most frequently used experimental animals in biological experiments. Experimental mice refer to white-coated rats or mice commonly used in physiology, medicine, pharmacy and other disciplines for teaching and research as experimental animals. Experimental mice have been developed into many types through long-term selection and directional breeding by humans. Generally, they are divided into two types: ordinary experimental mice and special experimental mice to meet special needs. Special experimental mice include high-cancer mice, low-cancer mice, diabetic mice, and congenital muscular atrophy mice. Some classify experimental mice into inbred lines, mutant lines, outbred and hybrid groups according to different crossbreeding methods and genetic characteristics. When performing embryo transfer on mice, the operator needs to use an embryo transfer device to extract healthy embryos and then inject them into the mother's body.

[0003] A mouse embryo transfer device of patent number CN201420150543.7 has the following disadvantages in actual use:

[0004] The above device has good sterile control effect, stable quality, and is easy to observe and collect embryos and transplant under an inverted microscope or a viewing mirror. It is simple to prepare and has low cost, but it needs to be fine-tuned when extracting and pushing out embryos, which is difficult to operate. SUMMARY

[0005] In order to improve the technical problem of high operation difficulty when the embryo transfer device is fine-tuned, the present application provides a mouse embryo transfer device.

[0006] The mouse embryo transfer device provided by the present application adopts the following technical scheme:

[0007] A mouse embryo transfer device includes a syringe shell, a connecting head is integrally formed at one end of the syringe shell, an embryo extraction tube is connected to the connecting head in interference, a coarse adjustment piston is slidably arranged inside the syringe shell, a plurality of sealing rings are integrally formed on the outer side of the coarse adjustment piston, and a coarse adjustment tube for moving the coarse adjustment piston is connected to the coarse adjustment piston.

[0008] A fine adjustment piston is slidably arranged on the inner side of the coarse adjustment tube, and a fine adjustment rod for moving the fine adjustment piston is connected to the fine adjustment piston.

[0009] By adopting the technical scheme, the position of the coarse adjustment piston is adjusted by the coarse adjustment tube, the embryo suction tube is greatly changed, coarse adjustment is performed when the embryo is sucked or pushed out, the position of the fine adjustment piston is adjusted by the fine adjustment rod, the embryo suction tube is slightly changed, and the adjustment is more convenient and the operation difficulty is reduced when slight adjustment is performed.

[0010] Preferably, a fixing ring is fixed to the outside of the injector housing, two symmetrical clamping rings are connected to the fixing ring, the end of the coarse adjustment tube is fixed with a locking ring, the outside of the locking ring is fixed with a coarse adjustment ring, the end of the fine adjustment rod away from the fine adjustment piston is fixed with a connecting rod, and the connecting rod is fixed with a fine adjustment ring.

[0011] By adopting the technical scheme, when the operator uses the embryo injector with one hand, the operator can hold the injector housing with the index finger and the middle finger and adjust the fine adjustment ring and the coarse adjustment ring with the thumb, so that the embryo injector can be used with one hand when sucking and pushing out, and the use is more convenient.

[0012] Preferably, a rubber ring for stabilizing the coarse adjustment tube is fixed to the inside of the tail of the injector housing, a circular hole is formed in the middle of the rubber ring, the coarse adjustment tube passes through the circular hole, a plurality of material segment holes are formed in the rubber ring in a circumferential direction, a limiting groove is formed in the inner wall of the circular hole, and a limiting strip is arranged on the outside of the coarse adjustment tube and passes through the limiting groove.

[0013] By adopting the technical scheme, the position of the coarse adjustment tube can be stabilized by the rubber ring, the coarse adjustment tube is prevented from being inclined, the limiting strip is staggered with the limiting strip when the coarse adjustment tube is rotated during fine adjustment, the friction is increased, the coarse adjustment tube is locked, and the fine adjustment is more stable.

[0014] Preferably, a first chamfer is arranged on one side of the limiting groove, and a second chamfer is arranged on one side of the limiting strip.

[0015] By adopting the technical scheme, the first chamfer and the second chamfer are staggered with each other when the coarse adjustment tube is rotated, so that the rotation is smoother and the operation is more fluent.

[0016] Preferably, a through hole is formed in the middle of the coarse adjustment piston, a widening groove is arranged in the middle of the through hole, the coarse adjustment tube passes through the through hole, a thickening segment is arranged on the coarse adjustment tube, the thickening segment is clamped in the widening groove, a blocking ring is further fixed to the outside of the coarse adjustment tube, and the blocking ring abuts against the coarse adjustment piston.

[0017] By adopting the technical scheme, the thickening segment can prevent the coarse adjustment piston from being separated from the coarse adjustment tube, the coarse adjustment piston can be connected with the coarse adjustment tube first when the equipment is assembled, and then the coarse adjustment piston is installed in the injector housing, and the assembly steps are convenient.

[0018] Preferably, the embryo suction tube is composed of the first thin tube and the second thin tube, the first thin tube and the second thin tube are integrally formed, and the included angle between the first thin tube and the second thin tube is 110°.

[0019] By adopting the technical scheme, the angle facilitates manual embryo suction under an inverted microscope and a stereoscope, and further facilitates operation.

[0020] Preferably, the front end of the second thin tube is provided with a tube tip port, and the outer side of the front end of the second thin tube is provided with an anti-skid ring.

[0021] By adopting the technical scheme, when the second thin tube is inserted into the uterus of a mouse, the tube tip port can be conveniently inserted, and when being inserted, the anti-skid ring can prevent the oviduct from sliding out of the second thin tube.

[0022] Preferably, the end of the first thin tube is provided with a widened shell convenient for hand holding, a hose is connected to the widened shell, and the hose is fitted on the outer side of the connector.

[0023] By adopting the technical scheme, when in use, the first thin tube is connected with the syringe shell, which facilitates separate packaging and use of the equipment.

[0024] Preferably, the length of the hose is greater than the length of the connector.

[0025] By adopting the technical scheme, after the hose and the connector are connected, the hose has a surplus length, can be bent at the joint, can slow down shaking, and further reduces the operation difficulty.

[0026] In summary, the present application has at least one of the following beneficial technical effects:

[0027] 1. The operator can adjust the position of the coarse adjustment piston through the coarse adjustment rod, so that the embryo suction tube changes greatly, and the embryo suction tube is coarsely adjusted when the embryo is sucked or pushed out. The position of the fine adjustment piston is adjusted through the fine adjustment rod, so that the embryo suction tube changes slightly, which is more convenient and reduces the operation difficulty. BRIEF DESCRIPTION OF DRAWINGS

[0028] Figure 1 It is a whole axonometric structural schematic diagram of the present application;

[0029] Figure 2 It is a structural schematic diagram of the outside of the syringe shell in the present application;

[0030] Figure 3 It is a structural schematic diagram of the inside of the syringe shell in the present application;

[0031] Figure 4 It is a sectional structural schematic diagram of the coarse adjustment piston in the present application;

[0032] Figure 5 This is a schematic diagram of the structure of the rubber ring in this application;

[0033] Figure 6 For this application Figure 5 Enlarged structural diagram at point A;

[0034] Figure 7 This is a schematic diagram of the internal structure of the coarse adjustment tube in this application;

[0035] Figure 8 This is a schematic diagram of the embryo aspiration tube in this application.

[0036] Reference numerals: 1. Syringe housing; 2. Connector;

[0037] 3. Embryo aspiration tube; 301. Thin tube one; 302. Thin tube two; 303. Tube tip; 304. Anti-slip ring; 305. Widened shell; 306. Flexible tube;

[0038] 4. Coarse adjustment piston; 5. Sealing ring; 6. Coarse adjustment tube; 7. Fine adjustment piston; 8. Fine adjustment rod; 9. Fixing ring; 10. Clamping ring; 11. Locking ring; 12. Coarse adjustment ring; 13. Connecting rod; 14. Fine adjustment ring; 15. Rubber ring; 16. Round hole; 17. Limiting groove; 18. Limiting strip; 19. Material-saving hole; 20. First chamfer; 21. Second chamfer; 22. Through hole; 23. Widening groove; 24. Thickened section; 25. Retaining ring. Detailed Implementation

[0039] The following is in conjunction with the appendix Figures 1-8 This application will be described in further detail.

[0040] This application discloses a mouse and rat embryo transfer device.

[0041] Example 1

[0042] Reference Figure 1 , 2, 3, 5, 6, 7, a mouse embryo transfer device, comprising a syringe shell 1, the tail of the syringe shell 1 is fixed with a rubber ring 15, a circular hole 16 is formed in the middle of the rubber ring 15, a plurality of circumferentially distributed material holes 19 are formed on the rubber ring 15, a limiting groove 17 is formed on the inner wall of the circular hole 16, a first chamfer 20 is arranged on one side of the limiting groove 17, a fixed ring 9 is fixed on the outer side of the syringe shell 1, two symmetrical clamping rings 10 are connected to the fixed ring 9, the clamping ring 10 can be used for clamping the syringe shell 1 by the index finger and the middle finger of the operator during use, a connecting head 2 is integrally formed at one end of the syringe shell 1, an embryo suction tube 3 is connected to the connecting head 2 in an interference fit, a coarse adjustment piston 4 is slidably arranged in the syringe shell 1, a plurality of sealing rings 5 are integrally formed on the outer side of the coarse adjustment piston 4, a coarse adjustment tube 6 is connected to the coarse adjustment piston 4 for moving the coarse adjustment piston 4, a locking ring 11 is fixed at one end of the coarse adjustment tube 6, a coarse adjustment ring 12 is fixed on the outer side of the locking ring 11, the coarse adjustment ring 12 is used for coarse adjustment by the thumb of the operator, the coarse adjustment tube 6 passes through the circular hole 16, a limiting strip 18 is arranged on the outer side of the coarse adjustment tube 6, a second chamfer 21 is arranged on one side of the limiting strip 18, the limiting strip 18 passes through the limiting groove 17, a fine adjustment piston 7 is slidably arranged on the inner side of the coarse adjustment tube 6, a fine adjustment rod 8 is connected to the fine adjustment piston 7 for moving the fine adjustment piston 7, a connecting rod 13 is fixed on one end of the fine adjustment rod 8 away from the fine adjustment piston 7, a fine adjustment ring 14 is fixed on the connecting rod 13, the fine adjustment ring 14 is used for fine adjustment by the thumb of the operator.

[0043] Through the above arrangement, when the operator uses the embryo injector with one hand, the syringe shell 1 can be clamped by the index finger and the middle finger, and the fine adjustment ring 14 and the coarse adjustment ring 12 can be adjusted by the thumb, the position of the coarse adjustment piston 4 is adjusted by the coarse adjustment tube 6, the embryo suction tube 3 is greatly changed, coarse adjustment is performed when the embryo is sucked or pushed out, and the position of the fine adjustment piston 7 is adjusted by the fine adjustment rod 8, the embryo suction tube 3 is slightly changed, the adjustment is more convenient and the operation difficulty is reduced, and when fine adjustment is performed, the coarse adjustment tube 6 can be rotated, the limiting strip 18 is offset from the limiting strip 17, the friction force is increased, the coarse adjustment tube 6 is locked, and the fine adjustment is more stable.

[0044] Refer to Figure 4 A through hole 22 is formed in the middle of the coarse adjustment piston 4, a widening groove 23 is arranged in the middle of the through hole 22, the coarse adjustment tube 6 passes through the through hole 22, and a thickening section 24 is arranged on the coarse adjustment tube 6, the thickening section 24 is clamped in the widening groove 23, and a stop ring 25 is further fixed on the outer side of the coarse adjustment tube 6 and abuts against the coarse adjustment piston 4.

[0045] Through the above setting, when assembling the device, the coarse adjustment piston 4 can be connected with the coarse adjustment tube 6 first, and then the coarse adjustment piston 4 is installed in the syringe shell 1. Under the limiting action of the syringe shell 1, the coarse adjustment piston 4 cannot expand, the thickening section 24 can prevent the coarse adjustment piston 4 from being separated from the coarse adjustment tube 6, and the blocking ring 25 can be in abutment with the coarse adjustment piston 4 to improve the sealing performance.

[0046] With reference to Figure 8 The embryo suction tube 3 is composed of a first thin tube 301 and a second thin tube 302, the first thin tube 301 and the second thin tube 302 are integrally formed, the included angle between the first thin tube 301 and the second thin tube 302 is 110°, the front end of the second thin tube 302 is provided with a tube tip port 303, the outer side of the front end of the second thin tube 302 is provided with an anti-skid ring 304, the end of the first thin tube 301 is provided with a widened shell 305 for convenient hand holding, the widened shell 305 is connected with a hose 306, the hose 306 is in interference fit on the outer side of the connecting head 2, and the length of the hose 306 is greater than the length of the connecting head 2.

[0047] Through the above setting, when using, the first thin tube 301 is connected with the syringe shell 1, and when the second thin tube 302 is inserted into the uterus of a mouse after sucking the embryo, the tube tip port 303 can be conveniently inserted, and at the same time, the anti-skid ring 304 can prevent the oviduct from sliding out of the second thin tube 302, and at the same time, the included angle between the first thin tube 301 and the second thin tube 302 is 110°, which is convenient for manually sucking the embryo under an inverted microscope and a stereoscope, and is convenient for the operator to observe, and after the hose 306 is connected with the connecting head 2, the hose 306 has a surplus length, which can be bent at the joint, can slow down the shaking, and further reduces the operation difficulty.

[0048] The principle of the mouse embryo transfer device is as follows: in use, the first tube 301 is connected with the syringe shell 1, after the embryo is sucked, the operator uses the embryo syringe with one hand, the first tube 301 and the second tube 302 are clamped at an angle of 110°, the angle is convenient for the operator to observe under the inverted microscope and the stereoscope, and the soft tube 306 has a surplus length after being connected with the connector 2, the surplus length can be bent at the joint, the shaking can be slowed down, after being inserted, the thumb is used to adjust the fine adjustment ring 14 and the coarse adjustment ring 12 respectively, the position of the coarse adjustment piston 4 is adjusted through the coarse adjustment tube 6, the embryo suction tube 3 is greatly changed, the coarse adjustment is performed when the embryo is sucked or pushed out, the position of the fine adjustment piston 7 is adjusted through the fine adjustment rod 8, the embryo suction tube 3 is slightly changed, the coarse adjustment tube 6 is rotated when the fine adjustment is performed, the limiting strip 18 is staggered with the limiting strip 17, the friction force is increased, the coarse adjustment tube 6 is locked, the fine adjustment is more stable, more convenient, and the operation difficulty is reduced.

[0049] In summary, the device can be adjusted with different sensitivities through the coarse adjustment tube 6 and the fine adjustment rod 8, the adjustment precision is improved when the embryo suction tube 3 needs to be slightly adjusted, the operation precision of the operator is reduced, the operation difficulty is reduced, and the use is more convenient.

[0050] The above are preferred embodiments of the application, and do not limit the protection scope of the application, therefore: any equivalent changes made on the structure, shape and principle of the application should be covered in the protection scope of the application.

Claims

1. A mouse embryo transfer device, comprising a syringe shell (1), an end of the syringe shell (1) is integrally formed with a connecting head (2), the embryo suction tube (3) is interference connected on the connecting head (2), a coarse adjustment piston (4) is slidably arranged in the syringe shell (1), characterized in that: The rough adjustment piston (4) is integrally formed with a plurality of sealing rings (5) on the outside, and a rough adjustment tube (6) is connected to the rough adjustment piston (4) for moving the rough adjustment piston (4); A fine adjustment piston (7) is slidably arranged on the inside of the rough adjustment tube (6), and a fine adjustment rod (8) is connected to the fine adjustment piston (7) for moving the fine adjustment piston (7).

2. The mouse embryo transfer device of claim 1, wherein: A fixing ring (9) is fixed on the outside of the injector housing (1), two symmetrical clamping rings (10) are connected to the fixing ring (9), an end of the rough adjustment tube (6) is fixed with a locking ring (11), a rough adjustment ring (12) is fixed on the outside of the locking ring (11), an end of the fine adjustment rod (8) away from the fine adjustment piston (7) is fixed with a connecting rod (13), and a fine adjustment ring (14) is fixed on the connecting rod (13).

3. The mouse embryo transfer device of claim 1, wherein: A rubber ring (15) for stabilizing the rough adjustment tube (6) is fixed on the inside of the tail of the injector housing (1), a circular hole (16) is formed in the middle of the rubber ring (15), the rough adjustment tube (6) passes through the circular hole (16), a plurality of material cutting holes (19) are formed on the rubber ring (15), a limiting groove (17) is formed on the inner wall of the circular hole (16), and a limiting strip (18) is arranged on the outside of the rough adjustment tube (6).

4. The mouse embryo transfer device of claim 3, wherein: The limiting groove (17) is provided with a first chamfer (20) on one side, and the limiting strip (18) is provided with a second chamfer (21) on one side.

5. The mouse embryo transfer device of claim 1, wherein: A through hole (22) is formed in the middle of the rough adjustment piston (4), a widening groove (23) is arranged in the middle of the through hole (22), the rough adjustment tube (6) passes through the through hole (22), and a thickening section (24) is arranged on the rough adjustment tube (6), the thickening section (24) is clamped in the widening groove (23), a stop ring (25) is further fixed on the outside of the rough adjustment tube (6), and the stop ring (25) abuts against the rough adjustment piston (4).

6. The mouse embryo transfer device of claim 1, wherein: The embryo suction tube (3) is composed of a first thin tube (301) and a second thin tube (302), the first thin tube (301) and the second thin tube (302) are integrally formed, and the included angle between the first thin tube (301) and the second thin tube (302) is 110°.

7. A mouse embryo transfer device according to claim 6, wherein: A tube tip port (303) is arranged at the front end of the second thin tube (302), and an anti-skid ring (304) is arranged on the outside of the front end of the second thin tube (302).

8. The mouse embryo transfer device of claim 6, wherein: A widened shell (305) for convenient hand holding is arranged at the end of the first thin tube (301), a hose (306) is connected to the widened shell (305), and the hose (306) is fitted on the outside of the connecting head (2).

9. A mouse embryo transfer device according to claim 8, wherein: The length of the hose (306) is greater than the length of the connecting head (2).

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