Multi-stage sterilizing and filtering device

Abstract

The utility model provides a multistage degerming and filtering device, which relates to the degerming and filtering field of non-sterile solutions, and comprises a main body unit, a liquid storage tank and an isolator, the main body unit comprises a first rubber tube, the surface of the first rubber tube is provided with a peristaltic pump, and one side of the isolator is provided with a stainless steel joint; according to the utility model, the first degerming-grade bag type filter and the liquid outlet head are connected in series through the second rubber tube, and the first degerming-grade bag type filter and the liquid outlet head are positioned in the isolator; a peristaltic pump is matched with a first rubber pipe, so that a non-sterile solution in a liquid storage tank can be conveyed into a first sterilization-stage bag type filter and a second sterilization-stage bag type filter, and the first sterilization-stage bag type filter and the second sterilization-stage bag type filter carry out one-time multi-stage sterilization filtration on the non-sterile solution to the interior of an isolator; and the sterilizing and filtering operation does not need to be repeatedly operated by personnel, so that the sterilizing and filtering convenience of the non-sterile solution is effectively improved.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the field of non-sterile solution's sterilization filtration, specifically a kind of multistage sterilization filtration device. BACKGROUND

[0002] In the production process of stem cell preparation, it is crucial to ensure a sterile environment, and auxiliary material solution and resuspension washing solution, as an important part of stem cell preparation, directly affect the quality and safety of the final product in sterility, therefore, these solutions need to be filtered by multistage sterilization to ensure their sterility.

[0003] When the auxiliary material solution and resuspension washing solution for stem cell preparation are subjected to sterilization filtration, a first sterilization filtration operation is first performed on a clean bench in a C-level environment, a sterilization grade capsule filter is connected to a platinum sulfided silica gel tube, the middle position of the platinum sulfided silica gel tube is fixed to the pump head of a peristaltic pump on the clean bench top, the power supply of the peristaltic pump is started, and the solution to be filtered is filtered into a sterile bottle, and a second sterilization filtration operation is then performed on a clean bench in a B-level environment.

[0004] However, in actual use, the commercial stem cell preparation production line is performed in an isolator in a C-level clean area, the isolator is a fully enclosed sterile environment, and therefore it is not possible to perform multistage sterilization filtration of non-sterile solution into the isolator in one step, thereby increasing the complexity of non-sterile solution filtration.

[0005] Therefore, the utility model provides a multistage sterilization filtration device to solve the above problems. UTILITY MODEL CONTENTS

[0006] To solve the above technical problems, the utility model provides the following technical scheme:

[0007] A multistage sterilization filtration device, comprising a main unit, a liquid storage tank and an isolator, the main unit comprises a first rubber tube, and the surface of the first rubber tube is provided with a peristaltic pump, one side of the isolator is provided with a stainless steel joint, one end of the stainless steel joint extends into the inner cavity of the isolator, first sterilization grade capsule filters are arranged on both sides of the inner cavity of the isolator, the second rubber tube is in communication between the stainless steel joint and the first sterilization grade capsule filter and between the first sterilization grade capsule filter and the second sterilization grade capsule filter, the other end of the second sterilization grade capsule filter is communicated with a liquid outlet head, and a liquid collecting tank is arranged in the inner cavity of the isolator.

[0008] Further, in the utility model, one end of the first rubber tube extends into the inner cavity of the liquid storage tank, the other end of the first rubber tube is connected to the stainless steel joint, and the first rubber tube and the stainless steel joint are in communication.

[0009] Further, in the utility model, the surface of the second rubber pipe is provided with the cable tie, the number of the cable tie is four, and evenly distributes at both sides of each second rubber pipe surface.

[0010] Further, in the utility model, the surface of the first sterilization grade capsule filter and the second sterilization grade capsule filter is communicated with the connecting pipe, the surface of the connecting pipe is movably connected with the round cover.

[0011] Further, in the utility model, the first rubber pipe and the second rubber pipe are platinum sulfurized silica gel pipes, the filter membrane pore size of the first sterilization grade capsule filter and the second sterilization grade capsule filter is 0.2 microns.

[0012] Further, in the utility model, one side of the stainless steel joint is fixedly connected with the sealing gasket, and the side, away from the stainless steel joint, of the sealing gasket is in contact with the isolator.

[0013] Further, in the utility model, the surface of the stainless steel joint and located at one side of the isolator is provided with the fastener, the fastener includes the first clamp and the second clamp, and the first clamp and the second clamp are movably connected through the pivot, one side of the second clamp is movably connected with the screw rod through the pivot, the bottom of the first clamp is movably connected with the connecting sleeve through the bearing, the bottom of the screw rod extends to the inner cavity of the connecting sleeve, and is threadedly connected with the inner cavity of the connecting sleeve.

[0014] Beneficial effects, the utility model has the following beneficial effects:

[0015] The utility model discloses a first sterilization grade capsule filter and liquid outlet head are connected in series through the second rubber pipe, and the first sterilization grade capsule filter and liquid outlet head are located inside the isolator, and the non-sterile solution in the liquid storage tank can be transmitted to the inside of the first sterilization grade capsule filter and the second sterilization grade capsule filter by using the peristaltic pump and the first rubber pipe, so that the first sterilization grade capsule filter and the second sterilization grade capsule filter perform disposable multi-stage sterilization filtration to the non-sterile solution inside the isolator, and the personnel need not repeatedly operate the sterilization filtration operation, and the convenience of non-sterile solution sterilization filtration is effectively improved. BRIEF DESCRIPTION OF DRAWINGS

[0016] Fig. 1 It is the main unit structure schematic view of the utility model;

[0017] Fig. 2 It is the sterilization filtration process route schematic view of the utility model;

[0018] Fig. 3 It is the stainless steel joint and fastener connection structure schematic view of the utility model.

[0019] In the drawing:

[0020] 1, the main unit; 11, the first rubber tube; 12, the peristaltic pump; 13, the stainless steel joint; 131, the gasket; 14, the second rubber tube; 141, the cable tie; 15, the first sterile grade capsule filter; 151, the connecting pipe; 16, the second sterile grade capsule filter; 17, the liquid outlet; 2, the liquid storage tank; 3, the isolator; 31, the fastener; 311, the first clamp; 312, the second clamp; 313, the connecting sleeve; 314, the screw rod; 4, the liquid collecting tank. DETAILED DESCRIPTION

[0021] In order to understand the technical content of the utility model more clearly, specific embodiments are described below with the accompanying drawings. In the present disclosure, aspects of the utility model are described with reference to the accompanying drawings, which show many embodiments of the description. The embodiments of the present disclosure are not necessarily defined in all aspects of the utility model. It should be understood that the various concepts and embodiments introduced above, as well as those described in more detail below, can be implemented in any of a number of ways, as the concepts and embodiments disclosed by the utility model are not limited to any implementation. In addition, some aspects of the utility model can be used alone, or in any appropriate combination with other aspects of the utility model.

[0022] Embodiment 1

[0023] As Figs. 1-3 shown, the first embodiment of the utility model provides a multi-stage sterilization filter device, which comprises a main unit 1, a liquid storage tank 2 and an isolator 3, the main unit 1 comprises a first rubber tube 11, and the surface of the first rubber tube 11 is provided with a peristaltic pump 12, one side of the isolator 3 is provided with a stainless steel joint 13, and one end of the stainless steel joint 13 extends to the inner cavity of the isolator 3, the inner cavity of the isolator 3 is provided with a first sterile grade capsule filter 15 on both sides, the second rubber tube 14 is communicated between the stainless steel joint 13 and the first sterile grade capsule filter 15 and between the first sterile grade capsule filter 15 and the second sterile grade capsule filter 16, the other end of the second sterile grade capsule filter 16 is communicated with a liquid outlet 17, and the inner cavity of the isolator 3 is provided with a liquid collecting tank 4.

[0024] As Figs. 1-3As shown, the first sterile-grade capsule filter 15 and the liquid outlet head 17 are connected in series through the second rubber tube 14, and the first sterile-grade capsule filter 15 and the liquid outlet head 17 are located inside the isolator 3. The non-sterile solution in the liquid storage tank 2 can be transmitted to the inside of the first sterile-grade capsule filter 15 and the second sterile-grade capsule filter 16 by using the peristaltic pump 12 in cooperation with the first rubber tube 11, so that the first sterile-grade capsule filter 15 and the second sterile-grade capsule filter 16 perform one-time multi-stage sterile filtration on the non-sterile solution to the inside of the isolator 3. The non-sterile solution in the liquid storage tank 2 can be transmitted to the stainless steel joint 13 by the first rubber tube 11 through the operation of the peristaltic pump 12, the solution is transmitted to the inner cavity of the second rubber tube 14 on one side by the stainless steel joint 13, the solution is transmitted to the inside of the first sterile-grade capsule filter 15 by the second rubber tube 14, and after the first-stage sterile filtration, the solution is transmitted to the inner cavity of the second sterile-grade capsule filter 16 through the second rubber tube 14 on the other side, and then the second sterile-grade capsule filter 16 performs two-stage sterile filtration on the solution. The filtered solution is injected into the inner cavity of the liquid collecting tank 4 in the isolator 3 through the liquid outlet head 17, so that the solution realizes one-time multi-stage sterile filtration in the isolator 3, without the need for repeated operation of the sterile filtration operation by personnel, and the convenience of the non-sterile solution sterile filtration is effectively improved.

[0025] Embodiment 2

[0026] With reference to Fig. 1 and 2 This is the second embodiment of the present application, which is based on the previous embodiment.

[0027] In this embodiment, one end of the first rubber tube 11 extends to the inner cavity of the liquid storage tank 2, and the other end of the first rubber tube 11 is connected with the stainless steel joint 13, and the first rubber tube 11 and the stainless steel joint 13 are connected in communication.

[0028] The surface of the second rubber tube 14 is provided with a tie 141, and the number of the tie 141 is four, which are evenly distributed on both sides of the surface of each second rubber tube 14.

[0029] The surface of the first sterile-grade capsule filter 15 and the second sterile-grade capsule filter 16 is communicated with a connecting pipe 151, and the surface of the connecting pipe 151 is movably connected with a round cover.

[0030] As Fig. 1 and 2As shown, one end of the first rubber tube 11 extends to the inside of the liquid storage tank 2, and the other end is in communication with the stainless steel joint 13, thereby facilitating the transmission of non-sterile solution inside the liquid storage tank 2, and facilitating the transmission of non-sterile solution to the inner cavity of the stainless steel joint 13. The second rubber tube 14 is provided with a strap 141 on the surface, thereby locking the connection between the second rubber tube 14 and the stainless steel joint 13, the first sterilization level capsule filter 15 and the second sterilization level capsule filter 16, ensuring the sealing of the connection, and effectively preventing leakage during solution transmission. The connection pipe 151 facilitates the discharge of impurities filtered inside the first sterilization level capsule filter 15 and the second sterilization level capsule filter 16, and can clean the inside of the first sterilization level capsule filter 15 and the second sterilization level capsule filter 16 through the connection pipe 151, improving the convenience of cleaning the inside of the first sterilization level capsule filter 15 and the second sterilization level capsule filter 16. The connection pipe 151 is closed by the round cover to prevent the solution from being discharged through the connection pipe 151 during sterilization and filtration of the solution.

[0031] Embodiment 3

[0032] Refer to Fig. 1 and 3 This is the third embodiment of the present application, which is based on the previous two embodiments.

[0033] In this embodiment, the first rubber tube 11 and the second rubber tube 14 are both platinum vulcanized silicone rubber tubes, and the filter membrane pore size of the first sterilization level capsule filter 15 and the second sterilization level capsule filter 16 is 0.2 microns.

[0034] The stainless steel joint 13 is fixedly connected with a sealing gasket 131 on one side, and the side away from the stainless steel joint 13 is in contact with the isolator 3.

[0035] The surface of the stainless steel joint 13 and located on one side of the isolator 3 is provided with a fastener 31, the fastener 31 includes a first clamp 311 and a second clamp 312, and the first clamp 311 and the second clamp 312 are movably connected through a shaft, the second clamp 312 is movably connected with a screw rod 314 on one side through a shaft, the bottom of the first clamp 311 is movably connected with a connecting sleeve 313 through a bearing, the bottom of the screw rod 314 extends to the inner cavity of the connecting sleeve 313, and is screwed with the inner cavity of the connecting sleeve 313.

[0036] As Fig. 1 and 3As shown, the filter membrane pore size of the first and second sterile grade capsule filter 15 and 16 is 0.2 microns, which can effectively block most bacteria and some viruses, and ensure that the filtered liquid meets the sterile standard. When the stainless steel connector 13 is connected to the isolator 3, one end of the stainless steel connector 13 is inserted into the reserved hole provided on one side of the isolator 3, and then one end of the stainless steel connector 13 enters the inner cavity of the isolator 3 through the reserved hole, which facilitates the transmission of non-sterile solution to the inside of the isolator 3. During the insertion process, the sealing gasket 131 is in contact with the isolator 3 driven by the stainless steel connector 13, which can ensure that the gap between the stainless steel connector 13 and the reserved hole is sealed, and the sterile environment inside the isolator 3 is ensured. After the stainless steel connector 13 is connected, it is preliminarily limited by the first clamp 311, and then the connecting sleeve 313 is rotated. Since the connecting sleeve 313 and the screw rod 314 are threadedly connected, the connecting sleeve 313 will drive the screw rod 314 to move downward while rotating, so that the screw rod 314 drives the second clamp 312 to move downward around the shaft axis, until the second clamp 312 is in close contact with the isolator 3, thereby realizing the fixation of the stainless steel connector 13 and preventing the stainless steel connector 13 from falling during use.

[0037] In use, firstly, the first hose 11 is connected to the peristaltic pump 12, with one end of the first hose 11 placed inside the reservoir 2 and the other end connected to the stainless steel connector 13. This connection facilitates solution transfer. Then, the stainless steel connector 13 is connected to the pre-drilled hole in the isolator 3, extending one end of the connector into the isolator 3. The stainless steel connector 13 is secured with fasteners 31 to prevent it from falling off later. Next, the second hose 14 is used to... The stainless steel connector 13, the first sterile capsule filter 15, and the second sterile capsule filter 16 are connected in series. During the series connection, the connection points are locked with cable ties 141 to prevent solution leakage during transmission. After the series connection is completed, the second sterile capsule filter 16 is removed, positioning the outlet 17 above the collection tank 4. Then, the peristaltic pump 12 is turned on. The operation of the peristaltic pump 12 allows the first tubing 11 to transfer the non-sterile solution inside the storage tank 2 to the stainless steel connector 13. The stainless steel connector 13 then transfers the solution... The solution is transferred to the inner cavity of the second tubing 14 on one side, allowing it to be conveyed to the interior of the first sterilization-grade capsule filter 15 for primary sterilization filtration. After primary sterilization filtration, the solution is then transferred through the other side of the second tubing 14 to the inner cavity of the second sterilization-grade capsule filter 16, where it undergoes secondary sterilization filtration. The filtered solution is then injected through the outlet 17 into the inner cavity of the collection tank 4 inside the isolator 3, thus achieving multi-stage filtration of the solution into the isolator 3 in a single pass. The solution is then transferred through the second tubing 14 to the first sterilization-grade capsule filter 15. The first sterile capsule filter 15 and the outlet head 17 are connected in series, and the first sterile capsule filter 15 and the outlet head 17 are located inside the isolator 3. By using the peristaltic pump 12 in conjunction with the first rubber tube 11, the non-sterile solution inside the storage tank 2 can be transferred to the first sterile capsule filter 15 and the second sterile capsule filter 16. This allows the first sterile capsule filter 15 and the second sterile capsule filter 16 to perform multi-stage sterilization filtration of the non-sterile solution in one go before it enters the isolator 3. This eliminates the need for repeated sterilization filtration operations by personnel and effectively improves the convenience of sterilization filtration of non-sterile solutions.

[0038] All standard parts used in this application can be purchased from the market, and can be customized according to the description and drawings. The specific connection methods of each part adopt conventional methods such as bolts, rivets, and welding that are mature in the prior art. The machinery, parts and equipment adopt conventional models in the prior art. The control method is automatic control through a controller. The control circuit of the controller can be implemented by simple programming by those skilled in the art and is common knowledge in the field. Since this application is mainly used to protect mechanical devices, the control method and circuit connection will not be explained in detail in this application.

[0039] Although the utility model has disclosed as above with preferable embodiments, it is not used to limit the utility model. Those skilled in the art to which the utility model belongs can make various changes and decorations without departing from the spirit and scope of the utility model. Therefore, the protection scope of the utility model is accurate according to the definition of the claims.

Claims

1. A multi-stage sterile filtration device comprising a main unit (1), a reservoir (2) and an isolator (3), characterized in that: The main unit (1) includes a first rubber tube (11), and the surface of the first rubber tube (11) is provided with a peristaltic pump (12); one side of the isolator (3) is provided with a stainless steel connector (13), and one end of the stainless steel connector (13) extends to the inner cavity of the isolator (3); the inner cavity of the isolator (3) is provided with a first sterilization-grade capsule filter (15) on both sides; the second rubber tube (14) is in communication with the first sterilization-grade capsule filter (15) and the second sterilization-grade capsule filter (16) between the stainless steel connector (13) and the first sterilization-grade capsule filter (15); the other end of the second sterilization-grade capsule filter (16) is in communication with a liquid outlet head (17); and the inner cavity of the isolator (3) is provided with a liquid collecting tank (4).

2. The multi-stage sterile filtration device of claim 1, wherein: One end of the first rubber tube (11) extends to the inner cavity of the liquid storage tank (2), and the other end of the first rubber tube (11) is connected with the stainless steel connector (13), and the first rubber tube (11) and the stainless steel connector (13) are in communication.

3. The multi-stage sterile filtration device of claim 1, wherein: The surface of the second rubber tube (14) is provided with a cable tie (141), and the number of the cable tie (141) is four, which are evenly distributed on both sides of the surface of each second rubber tube (14).

4. The multi-stage sterile filtration device of claim 1, wherein: The surface of the first sterilization-grade capsule filter (15) and the second sterilization-grade capsule filter (16) is in communication with a connecting pipe (151), and the surface of the connecting pipe (151) is movably connected with a round cover.

5. The multi-stage sterile filtration device of claim 1, wherein: The first rubber tube (11) and the second rubber tube (14) are platinum sulfurized silicone rubber tubes, and the filter membrane pore size of the first sterilization-grade capsule filter (15) and the second sterilization-grade capsule filter (16) is 0.2 microns.

6. The multi-stage sterile filtration device of claim 1, wherein: One side of the stainless steel connector (13) is fixedly connected with a sealing gasket (131), and the side, away from the stainless steel connector (13), of the sealing gasket (131) is in contact with the isolator (3).

7. The multi-stage sterile filtration device of claim 1, wherein: The surface of the stainless steel connector (13) and located on one side of the isolator (3) is provided with a fastener (31), the fastener (31) includes a first clamp (311) and a second clamp (312), and the first clamp (311) and the second clamp (312) are movably connected through a rotating shaft, one side of the second clamp (312) is movably connected with a screw rod (314) through a rotating shaft, the bottom of the first clamp (311) is movably connected with a connecting sleeve (313) through a bearing, the bottom of the screw rod (314) extends to the inner cavity of the connecting sleeve (313) and is threadedly connected with the inner cavity of the connecting sleeve (313).

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