Separation device for recombinant protein

Abstract

The utility model belongs to the technical field of separation devices for recombinant proteins, and particularly relates to a separation device for recombinant proteins, which comprises a kettle body, a first feeding pipe is arranged on the edge of one side of the top end of the kettle body, and a second feeding pipe is rotatably arranged in the middle of the top end of the kettle body in a penetrating manner; a salt solution or particle salt is continuously put into the kettle body through the second feeding pipe, and the salt solution or particle salt is discharged through the multiple groups of scattering pipes with different lengths and enters different positions of the kettle body, so that the salt is more uniformly distributed in the kettle body, the reaction efficiency is improved, and the problem that the salt concentration is too high due to partial region solution concentration is solved; meanwhile, the output end of the motor is matched with the first belt group to drive the plurality of groups of stirring rods to synchronously rotate, so that each stirring rod can generate shearing force and extrusion force, a more complex flowing mode is formed in the kettle body, eddy current is effectively broken and dispersed, salt is more uniformly distributed in the kettle body, and the salt content is increased. Therefore, the mixing uniformity is improved.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the technical field of separation device for recombinant protein, specifically a separation device for recombinant protein. BACKGROUND

[0002] Recombinant protein refers to the protein obtained and produced by DNA recombination technology or recombination RNA technology, and through the separation and purification process, impurities and other non-target proteins can be removed, thereby improving the purity and quality of the recombinant protein.

[0003] Chinese patent (publication number: CN221254462U) discloses a protein separation device for recombinant protein, which comprises a bottom plate, a support plate is arranged on one side above the bottom plate, two mounting plates are arranged on one side of the upper end of the support plate, a salt-out kettle is fixedly installed on the two mounting plates, a liquid guide pipe is arranged at the middle below the salt-out kettle, the liquid guide pipe is communicated with a filter box below, a motor is arranged at the middle above the salt-out kettle, the rotating shaft of the motor penetrates the salt-out kettle and is fixedly connected with a stirring rod, a plurality of equidistantly arranged stirring blades are arranged on the stirring rod, a cover plate is arranged on one side of the filter box, an installation frame is arranged at one end of the filter box in the filter box, a filter screen is arranged at the lower end in the installation frame, and a pressurizing pipe is arranged on one side of the upper end of the salt-out kettle. The separation device has simple structure, convenient operation, fast mixing speed of salt solution and protein, effectively shortens the salt-out speed of protein by pressurizing, and improves the separation efficiency of protein.

[0004] The protein and the salt solution of the above-mentioned protein separation device for recombinant protein are sequentially added to the inside of the salt-out kettle through the liquid inlet pipe, and the liquid inlet pipe is fixedly installed on one side of the top end of the salt-out kettle. When the salt solution enters the inside of the salt-out kettle through the liquid inlet pipe, the salt solution is easily concentrated and distributed in a certain area of the salt-out kettle, causing the solution concentration in some areas to be too high. Even if the solution is subsequently stirred by the stirring blades, the solution concentration in some areas is too high, which easily causes the stirring resistance to increase, so that the stirring blades cannot completely mix the solution uniformly, thereby affecting the full mixing of the protein and the salt solution and the subsequent separation effect. UTILITY MODEL CONTENTS

[0005] In order to make up for the deficiencies of the prior art and solve at least one technical problem proposed in the background art, the utility model provides a separation device for recombinant protein.

[0006] The utility model discloses a technical scheme that solves its technical problem is adopted: a kind of separating device for recombinant protein, including kettle body, first feeding pipe is equipped in the side edge of kettle body top, and second feeding pipe is rotatably penetrated and equipped in the middle of kettle body top;The kettle body side is communicated with pressurizing pipe;The kettle body top is communicated with drain pipe, and valve is equipped on drain pipe;Filtering box is placed in the kettle body bottom;Multiple groups of scattering pipes are communicated and inclined around the one end of second feeding pipe, and the length of each group of scattering pipes is different;Multiple groups of stirring rods are rotatably penetrated and equipped around the inner edge of kettle body, and are driven connected by first belt group between multiple groups of stirring rods;The one end of stirring rod is connected with motor output end, and one end of a group of stirring rods is driven connected with second feeding pipe by second belt group.

[0007] Preferably, the kettle body is internally mounted with a gas delivery ring by a connecting rod; multiple groups of branch pipes are communicated and surrounded on one side of the gas delivery ring, and the pressurizing pipe is communicated with the gas delivery ring.

[0008] Preferably, the filtering box is internally fixed with a support frame; an installation frame is placed on the support frame, and a filter screen is arranged at one end of the installation frame; the contact surfaces of the support frames are both provided with corresponding magnets.

[0009] Preferably, a support frame is mounted on one side of the kettle body top; baffles are arranged on one end of the first feeding pipe and the second feeding pipe by sliding cover, and the two groups of baffles are connected by a support; a screw rod is threadedly penetrated in one end of the support frame, and the other end of the screw rod is rotatably connected with the support; a guide rod is slidably penetrated in one end of the support frame, and the other end of the guide rod is fixedly connected with the support.

[0010] Preferably, a guide cylinder is communicated with the kettle body bottom, and the guide cylinder is provided in the form of a topless cone; one end of the drain pipe is communicated with the guide cylinder.

[0011] Preferably, an observation window is arranged on one side of the kettle body, and the observation window is transparent.

[0012] Preferably, a support leg is mounted on one end of the outer wall of the kettle body, and a rubber pad is arranged at the bottom of the support leg.

[0013] The utility model has the advantages of:

[0014] The utility model provides a separation device for recombination protein, through second feed pipe to the kettle body inside sustainedly put salt solution or granular salt, at this moment salt solution or granular salt will discharge through different long short multiple groups of scattering material pipe, into the kettle body different position, make the distribution of salt in the kettle body more uniform, improve reaction efficiency, reduce the stirring resistance increase caused by partial area solution concentration too high, simultaneously through motor output end cooperation first belt set drive multiple groups of stirring rod synchronous rotation, make every stirring rod can produce shear force and extrusion pressure, to form more complex flow pattern in the kettle body, effectively break and scatter vortex, make salt more evenly distribute in the kettle body, to improve the uniformity of mixing, and a group of stirring rod passes through second belt set drive second feed pipe synchronous rotation, make multiple groups of scattering material pipe follow its rotation, further promote the uniform distribution of salt, to realize the uniform distribution of salt and efficient reaction.

[0015] The utility model provides a separation device for recombination protein, through the pressure pipe to the gas ring inside delivery pressure gas, such as nitrogen, at this moment pressurized gas flows out through a plurality of branch pipes, make each part in the kettle body can receive uniform pressure, thereby improve separation effect. BRIEF DESCRIPTION OF DRAWINGS

[0016] The drawings described herein are used to provide further understanding of the utility model, and constitute a part of the present application, the illustrative embodiment of the utility model and its explanation are used to explain the utility model, and do not constitute undue limitation to the utility model. In the drawings,

[0017] Figure 1 It is the perspective drawing of the utility model,

[0018] Figure 2 It is the cross section drawing of the utility model,

[0019] Figure 3 It is the connection schematic drawing of first belt set and second belt set in the utility model,

[0020] Figure 4 It is the perspective drawing of filter box in the utility model,

[0021] Figure 5 It is the perspective drawing of baffle in the utility model.

[0022] Legend:

[0023] 1, kettle body; 2, first feed pipe; 3, second feed pipe; 4, spreader pipe; 5, stirring rod; 6, first belt set; 7, motor; 8, second belt set; 9, filter box; 10, connecting rod; 11, gas delivery ring; 12, branch pipe; 13, pressurizing pipe; 14, support frame; 15, mounting frame; 16, filter screen; 17, baffle; 18, support frame; 19, screw; 20, guide rod; 21, guide cylinder; 22, liquid discharge pipe; 23, observation window. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of the present application.

[0025] Specific embodiments are given below.

[0026] Please refer to Figures 1-4 The present application provides a kind of recombination protein separation device, including kettle body 1, the first feed pipe 2 is equipped with in the top end one side edge of kettle body 1, and the second feed pipe 3 is rotationally penetrated and equipped in the top end middle of kettle body 1;The pressurizing pipe 13 is communicated in one side of kettle body 1;The kettle body 1 top end is communicated with liquid discharge pipe 22, and valve is equipped on liquid discharge pipe 22;The filter box 9 is placed in the bottom end of kettle body 1;The second feed pipe 3 one end is inclined and is communicated with multiple groups of spreader pipe 4, and the length of each group of spreader pipe 4 is different;Multiple groups of stirring rod 5 are rotationally penetrated and equipped in the inner edge of kettle body 1, and multiple groups of stirring rod 5 are driven and connected by first belt set 6 between them;The output end of motor 7 is connected with one end of stirring rod 5, and one end of one group of stirring rod 5 is driven and connected with second feed pipe 3 by second belt set 8.

[0027] When working, the first feeding pipe 2 is used for feeding protein materials, and the second feeding pipe 3 is used for feeding salt solution or granular salt. The salt solution or granular salt is continuously fed into the kettle body 1 through the second feeding pipe 3, and then is discharged through the multiple groups of different length and different length of the distribution pipes 4 into different positions of the kettle body 1, so that the distribution of the salt in the kettle body 1 is more uniform, the reaction efficiency is improved, the stirring resistance caused by the too high solution concentration in the partial area is reduced, and the motor 7 is driven by the first belt set 6 to drive the multiple groups of stirring rods 5 to rotate synchronously, so that each stirring rod 5 can generate shear force and extrusion force to form a more complex flow pattern in the kettle body 1, effectively break and disperse the vortex, and make the salt more uniformly distributed in the kettle body 1, so as to improve the uniformity of mixing. At the same time, the second feeding pipe 3 is driven by the second belt set 8 to rotate synchronously, so that the multiple groups of distribution pipes 4 rotate with it, further promoting the uniform distribution of the salt, so as to realize the uniform distribution of the salt and the efficient reaction.

[0028] Please refer to Figures 1-2 , the gas distribution ring 11 is installed in the kettle body 1 through the connecting rod 10; the gas distribution ring 11 is communicated with multiple branch pipes 12 on one side, and the pressurizing pipe 13 is communicated with the gas distribution ring 11; when working, one end of the pressurizing pipe 13 is connected with the pressurizing equipment, and the pressurizing pipe 13 is used for conveying pressurizing gas such as nitrogen into the gas distribution ring 11, so that the pressurizing gas flows out through the multiple branch pipes 12, so that each part of the kettle body 1 can be uniformly pressed, thereby improving the separation effect.

[0029] Please refer to Figure 1 、 Figure 4 , the support frame 14 is fixedly connected in the filter box 9; the mounting frame 15 is placed on the support frame 14, and the filter screen 16 is arranged at one end of the mounting frame 15; the support frame 14 and the support frame 14 are in contact with each other and are provided with attractive magnets; when working, the filter screen 16 can be used for filtering the protein liquid to realize the purpose of protein separation, the support frame 14 is used for supporting the mounting frame 15, and the two are movably connected, so that the support frame 14 can be conveniently taken and replaced, and the protein filtered on the surface of the filter screen 16 can be collected, and the magnet is used to improve the stability of the mounting frame 15 placed on the support frame 14.

[0030] Please refer to Figure 1 、 Figure 5The kettle body 1 top end one side is equipped with support frame 18;The first feeding pipe 2 and the second feeding pipe 3 one end are both equipped with baffle 17, and two groups of baffle 17 are connected through support;The support frame 18 one end is threaded through screw rod 19, and the other end of screw rod 19 is rotatably connected with support;The support frame 18 one end is slidably penetrated with guide rod 20, and the other end of guide rod 20 is fixedly connected with support;When working, baffle 17 is used to complete the feeding of raw materials or the device is placed, dust and impurities fall into the kettle body 1 through the feeding pipe, causing pollution, by rotating screw rod 19, under the guiding and limiting action of guide rod 20, baffle 17 can be close to the feeding pipe or away from it, to control the opening and closing of the feeding pipe.

[0031] Please refer to Figures 1-2 The kettle body 1 bottom end is communicated with guide cylinder 21, and guide cylinder 21 is provided with no top taper;The liquid discharge pipe 22 one end is communicated with guide cylinder 21;When working, through the inclined surface of guide cylinder 21, the phenomenon that liquid is deposited in the dead angle at the bottom end of kettle body 1 and is difficult to discharge can be reduced when the kettle body 1 is discharged, and the flow of the liquid discharge is improved.

[0032] Please refer to Figure 1 The kettle body 1 one side is equipped with observation window 23, and observation window 23 is transparent;When working, through observation window 23, personnel can check the inside of kettle body 1 at any time, the separation and mixing of protein solution.

[0033] Please refer to Figure 1 The kettle body 1 outer wall one end is equipped with support leg, and the support leg bottom is equipped with rubber pad;When working, the support leg is used to support the kettle body 1, and the rubber pad can increase the friction between the support leg and the ground, to improve the stability of the device.

[0034] Working principle: the first feeding pipe 2 is used for putting protein material, the second feeding pipe 3 is used for putting salt solution or granular salt, the salt solution or granular salt is continuously put into the kettle body 1 through the second feeding pipe 3, at this time, the salt solution or granular salt is discharged through multiple groups of different length and shortness of the material distribution pipe 4, enters different positions of the kettle body 1, makes the distribution of salt in the kettle body 1 more uniform, improves the reaction rate, reduces the stirring resistance caused by the solution concentration of partial area being too high, simultaneously, the motor 7 output end is matched with the first belt group 6 to drive multiple groups of stirring rods 5 to rotate synchronously, so that each stirring rod 5 can generate shear force and extrusion force, to form more complex flow pattern in the kettle body 1, effectively breaks and disperses vortex, makes the salt more uniformly distributed in the kettle body 1, to improve the uniformity of mixing, simultaneously, a group of stirring rods 5 drives the second feeding pipe 3 to rotate synchronously through the second belt group 8, so that multiple groups of material distribution pipes 4 rotate with it, further promotes the uniform distribution of salt, to realize the uniform distribution of salt and efficient reaction; one end of the pressurizing pipe 13 is connected with a pressurizing device, pressure gas such as nitrogen is transported into the gas distribution ring 11 through the pressurizing pipe 13, at this time, the pressurizing gas flows out through multiple branch pipes 12, so that each part in the kettle body 1 can be subjected to uniform pressure, thereby improving the separation effect; the filter screen 16 can filter the protein liquid, realizes the purpose of protein separation, the supporting frame 14 is used for supporting the mounting frame 15, the two movable connection can facilitate subsequent taking and replacing the supporting frame 14, and the protein filtered on the surface of the filter screen 16 is collected, and the magnet is used for improving the stability of the mounting frame 15 placed on the supporting frame 14; the baffle 17 is used for preventing dust and impurities from falling into the kettle body 1 through the feeding pipe when the device completes putting raw materials or the device is placed, causes pollution, through the rotating screw 19, under the guiding and limiting action of the guide rod 20, the baffle 17 can be close to or away from the feeding pipe, to control the opening and closing of the feeding pipe; through the inclined surface of the guide cylinder 21, the phenomenon that the liquid is deposited in the dead angle at the bottom end of the kettle body 1 and is difficult to discharge can be reduced, the flow of the liquid is improved; through the observation window 23, personnel can check the inside of the kettle body 1, the separation and mixing of the protein liquid at any time; the supporting leg is used for supporting the kettle body 1, and the rubber pad can increase the friction between the supporting leg and the ground, to improve the stability of the device.

[0035] The basic principle, main features and advantages of the present application are shown and described above. Those skilled in the art should understand that the present application is not limited by the above examples, and the above examples and descriptions in the specification are only to illustrate the principles of the present application. Without departing from the spirit and scope of the present application, various changes and improvements can be made to the present application, and these changes and improvements all fall within the scope of the claimed present application.

Claims

1. A kind of separating device for recombination protein, including kettle body (1), first feed pipe (2) is equipped with in the top end side edge of kettle body (1), and the second feed pipe (3) is rotatably penetrated in the top end middle of kettle body (1);The pressurizing pipe (13) is communicated with in the side of kettle body (1);The drain pipe (22) is communicated with in the top end of kettle body (1), and valve is equipped on drain pipe (22);Filtering box (9) is placed in the bottom end of kettle body (1);Its characterized in that: The second feeding pipe (3) one end of the inclined ring around the communication has a plurality of groups of scattering pipe (4), and each group of scattering pipe (4) length is different; The kettle body (1) inside edge ring around the rotation is provided with a plurality of groups of stirring rod (5), and a plurality of groups of stirring rod (5) are driven connected through the first belt group (6); The stirring rod (5) one end is connected with the motor (7) output, and one group of stirring rod (5) one end is driven connected with the second feeding pipe (3) through the second belt group (8).

2. The separation device for a recombinant protein according to claim 1, wherein: The kettle body (1) is internally provided with a gas distribution ring (11) through a connecting rod (10); The gas distribution ring (11) one side is communicated with a plurality of branch pipes (12) around, and the pressurizing pipe (13) one end is communicated with the gas distribution ring (11).

3. The separation device for a recombinant protein according to claim 1, wherein: The filter box (9) is internally fixed with a support frame (14); The support frame (14) is placed with a mounting frame (15), and the mounting frame (15) one end is provided with a filter screen (16); The contact surface of the support frame (14) and the support frame (14) is provided with a magnet.

4. The apparatus of claim 1, wherein: the first and second separation devices are each a chromatography column. The kettle body (1) top one side is provided with a support frame (18); The first feeding pipe (2) and the second feeding pipe (3) one end are both provided with a baffle (17) which is slidably covered, and the two baffles (17) are connected through the support; The support frame (18) one end is threaded through a screw rod (19), and the other end of the screw rod (19) is rotatably connected with the support; The support frame (18) one end is slidably through a guide rod (20), and the other end of the guide rod (20) is fixedly connected with the support.

5. The recombinant protein separation device as described in claim 1, characterized in that: The kettle body (1) bottom is communicated with a guide cylinder (21), and the guide cylinder (21) is provided without top taper; The liquid discharge pipe (22) one end is communicated with the guide cylinder (21).

6. The recombinant protein separation device as described in claim 1, characterized in that: The kettle body (1) one side is provided with an observation window (23), and the observation window (23) is transparently provided.

7. The apparatus of claim 1, wherein: the first and second separation elements are configured to separate the recombinant protein from the sample. The kettle body (1) one end is provided with a support leg, and the support leg bottom is provided with a rubber pad. The kettle body (1) one end is provided with a support leg, and the support leg bottom is provided with a rubber pad.

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