Chromatography and tangential flow filtration integrated device capable of switching flow paths

Abstract

The utility model discloses a chromatography and tangential flow filtration integrated device capable of switching flow paths, which comprises two pumps, a circulation tank, a circulation pipeline, a valve and a detection element, and a chromatography and tangential flow filtration structure is integrated on the same device. The types and the number of the circulation pipelines, the valves and the detection elements can be changed according to the process development requirements of chromatography or tangential flow filtration, the function can be rapidly switched by directly replacing the whole flow path and switching operation software, and the upper limit value adjustment of the working flow can be achieved. And the process development work originally completed by two devices can be realized by switching the flow path structure. The device can be used for chromatography and tangential flow filtration operation in a process development stage, is not only suitable for small-scale test process groping tests, but also can be used for preparation of a large number of samples and pilot scale amplification production operation, realizes dual purposes, is simple and rapid in the whole operation process, effectively improves the process development efficiency, and saves the use cost.

Description

Technical Field

[0001] This utility model relates to an integrated chromatography and tangential flow filtration device with switchable flow paths, belonging to the field of biopharmaceutical purification and separation technology. Background Technology

[0002] Chromatography is a technique for separating mixed components based on the difference in partition coefficients between the mobile and stationary phases. It is generally used for the analysis of organic compounds, metal ions, amino acids, etc. Tangential flow filtration is a technique for separating and purifying proteins and other biomolecules from mixtures such as cell culture media or lysates. It enables researchers in biochemistry, immunology, and microbiology to remove impurities while retaining the desired molecules. Chromatography and tangential flow filtration are two of the most commonly used purification techniques for biological macromolecules, and they have wide applications in the purification of antibodies, recombinant proteins, and viruses.

[0003] However, chromatography and tangential flow filtration technologies currently differ in their working principles, equipment flow paths, and operating software. To implement these two processes, both chromatography and tangential flow filtration devices are often required, necessitating the development of both purification processes. Both chromatography and tangential flow filtration require low-flow-rate equipment during the research and development phase. Once the process is scaled up for pilot production, higher-flow-rate equipment is needed. Since their tubing and sensor inner diameters are fixed, replacement with larger-flow-rate components or equipment is necessary, leading to a large number of required devices, significant space requirements, and high costs. Therefore, it is necessary to propose an integrated chromatography and tangential flow filtration device with switchable flow paths. This device allows for flow path switching by changing the tubing, and the control software enables a single device to perform both chromatography and tangential flow filtration functions. Summary of the Invention

[0004] The purpose of this invention is to solve the above-mentioned problems by providing an integrated chromatography and tangential flow filtration device with switchable flow paths, thereby addressing the issues of large equipment quantity, large area occupation, and high investment costs in the development of chromatography and tangential flow filtration processes, and improving performance.

[0005] The technical solution of this utility model is: an integrated chromatography and tangential flow filtration device with switchable flow paths. When the device adopts a chromatography flow path structure, its features are: the chromatography flow path structure includes a first pump body, a second pump body, a column position valve group, a detection sensor group, and a chromatography column; a first chromatography connecting pipe is connected to the first end of the first pump body, and several first valves are connected to the first chromatography connecting pipe; a second chromatography connecting pipe is connected to the second end of the first pump body; a third chromatography connecting pipe is connected to the first end of the second pump body, and the third chromatography connecting pipe is connected to... The system is equipped with several second valves. A fourth chromatography connecting pipe is connected to the second end of the second pump body. The fourth chromatography connecting pipe is connected to the first end of the second chromatography connecting pipe. A flow meter and a first pressure sensor are also provided on the fourth chromatography connecting pipe. The second end of the fourth chromatography connecting pipe is divided into a first branch pipe and a second branch pipe. The first end of the first branch pipe is connected to a bubble trap. The second end of the first branch pipe is connected to a detection sensor group and a chromatography column through a column position valve group.

[0006] Furthermore, in the aforementioned integrated chromatographic and tangential flow filtration device with switchable flow paths, the first branch of the fourth chromatographic connecting pipe is also connected to a fourth valve and a first normally closed valve, and the first branch of the fourth chromatographic connecting pipe located at both ends of the fourth valve is respectively connected to a tenth chromatographic connecting pipe, which is connected to the bubble trap through a double row of tenth chromatographic connecting pipes, and each tenth chromatographic connecting pipe is connected to a fifth valve; the second branch of the fourth chromatographic connecting pipe is connected to a third valve.

[0007] Furthermore, in the aforementioned integrated chromatography and tangential flow filtration device with switchable flow paths, the chromatography column and the column position valve group are cyclically connected through a fifth chromatography connecting pipe and a sixth chromatography connecting pipe.

[0008] Furthermore, in the aforementioned integrated chromatography and tangential flow filtration device with switchable flow paths, the detection sensor group in the chromatography flow path structure and the column position valve group are connected through a seventh chromatography connecting pipe, and a second normally closed valve is connected to the seventh chromatography connecting pipe.

[0009] Furthermore, in the aforementioned integrated chromatography and tangential flow filtration device with switchable flow paths, the detection sensor group in the chromatography flow path structure is also connected to an eighth chromatography connecting pipe, and several sixth valves are connected to the eighth chromatography connecting pipe.

[0010] Furthermore, in the aforementioned integrated chromatography and tangential flow filtration device with switchable flow paths, the column position valve group in the chromatography flow path structure is a diamond-shaped regulating valve composed of four single column position valves.

[0011] This utility model also provides an integrated chromatography and tangential flow filtration device with switchable flow paths. When the device adopts a tangential flow filtration flow path structure, the tangential flow filtration flow path structure includes a first pump body, a second pump body, a detection sensor group, and a circulation tank. The first end of the first pump body is provided with several first valves, and the second end of the first pump body is connected to the feed end of the circulation tank through a first tangential flow filtration connecting pipe. The first end of the second pump body is connected to a second tangential flow filtration connecting pipe, and the second tangential flow filtration connecting pipe is used to divert the flow of a second tangential flow filtration connecting pipe. The first branch pipe is connected to the second branch pipe of the second tangential flow filter connection pipe. The first branch pipe of the second tangential flow filter connection pipe is connected to the outlet end of the circulation tank. A second valve is connected to the first branch pipe of the second tangential flow filter connection pipe. A third tangential flow filter connection pipe is connected to the second end of the second pump body. The third tangential flow filter connection pipe is equipped with a flow meter and a first pressure sensor. The third tangential flow filter connection pipe is also branched by the first branch pipe of the third tangential flow filter connection pipe and the second branch pipe of the second tangential flow filter connection pipe. The second branch of the three-tangential flow filter connecting pipe is connected to the first branch of the third-tangential flow filter connecting pipe, which is also connected to a fourth valve and a first normally closed valve. The first branch of the third-tangential flow filter connecting pipe located at both ends of the fourth valve is connected to a sixth-tangential flow filter connecting pipe. These sixth-tangential flow filter connecting pipes are connected to the detection sensor group via two rows of them. Each sixth-tangential flow filter connecting pipe is connected to a fifth valve, and a membrane is provided between the detection sensor group and the fifth valve through this sixth-tangential flow filter connecting pipe. The return flow of the circulation tank... A fourth tangential flow filter connecting pipe is provided at the flow end. A metering control valve, a second conductivity sensor, and a third pressure sensor are sequentially connected to the fourth tangential flow filter connecting pipe. The fourth tangential flow filter connecting pipe is also divided into a first branch pipe and a second branch pipe. The first branch pipe is connected to the detection sensor group, and the second branch pipe is connected to the first branch pipe of the third tangential flow filter connecting pipe, for cyclically connecting the second pump body and the circulation tank.

[0012] Furthermore, in the aforementioned integrated chromatography and tangential flow filtration device with switchable flow paths, a single-position valve is connected to the first branch pipe of the fourth tangential flow filtration connecting pipe and the second branch pipe of the fourth tangential flow filtration connecting pipe.

[0013] Furthermore, in the aforementioned integrated chromatographic and tangential flow filtration device with switchable flow paths, the detection sensor group in the tangential flow filtration flow path structure is connected to the first branch of the fourth tangential flow filtration connecting pipe via a fifth tangential flow filtration connecting pipe, and a sixth valve is connected to the fifth tangential flow filtration connecting pipe.

[0014] Furthermore, in the aforementioned integrated chromatography and tangential flow filtration device with switchable flow paths, the detection sensor group includes several first conductivity sensors, ultraviolet sensors, pH sensors, and second pressure sensors.

[0015] The present invention integrates two pumps, a circulation tank, a flow pipeline, valves, and detection elements, combining chromatography and tangential flow filtration structures into a single device. The types and quantities of the flow pipeline, valves, and detection elements can be changed according to the process development requirements of chromatography or tangential flow filtration. It not only enables rapid switching of functions by directly replacing the overall flow path and switching the operation software, but also allows for adjustment of the upper limit of the working flow rate. By switching the flow path structure, the process development work that originally required two devices can be achieved.

[0016] Compared with existing technologies, by adopting the technical solution of this utility model, the process development work of chromatography and tangential flow filtration, which were originally completed by two devices, can be achieved by switching the flow path structure. This realizes dual-purpose use of one device, improves the utilization rate of the device, saves resources and reduces waste, and effectively saves experimental space. Moreover, the types and quantities of flow pipelines, valves and detection sensors can be changed according to the process development needs of chromatography or tangential flow filtration, thereby adjusting the upper limit of the working flow rate. This can meet various application scenarios from small-scale development to pilot-scale amplification, maximizing the working range and application scenarios of the device. Furthermore, the replaced flow pipelines, valves or detection sensors are all disposable flow path consumables that can be directly discarded after use, so as to save cleaning time, manpower and verification costs in pilot production. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the flow path structure of this utility model used in chromatography operations;

[0018] Figure 2 This is a schematic diagram of the flow path structure of this utility model for tangential flow filtration.

[0019] The meanings of the labels in the figures are as follows: 1-First pump body, 2-Second pump body, 3-First valve, 4-Second valve, 5-Flow meter, 6-First pressure sensor, 7-Third valve, 8-Fourth valve, 9-Fifth valve, 10-First normally closed valve, 11-Column position valve assembly, 111-Single column position valve, 12-Second normally closed valve, 13-Detection sensor assembly, 131-First conductivity sensor, 132-Ultraviolet sensor, 133-pH sensor, 134-Second pressure sensor, 14-Sixth valve, 15-Chromatography column, 16-Bubble trap, 17-Circulation tank, 18-Membrane pack, 19-Metering control valve, 20-Second conductivity sensor, 21-Third pressure sensor, 22-First chromatography connecting tube, 23-Second chromatography connecting tube, 24-Third chromatography connecting tube, 25-Fourth chromatography connecting tube, 26-57-58-58-59 ... 1-First branch of the fourth chromatographic connecting pipe, 252-Second branch of the fourth chromatographic connecting pipe, 26-Fifth chromatographic connecting pipe, 27-Sixth chromatographic connecting pipe, 28-Seventh chromatographic connecting pipe, 29-Eighth chromatographic connecting pipe, 30-Tenth chromatographic connecting pipe, 31-First tangential flow filter connecting pipe, 32-Second tangential flow filter connecting pipe, 321-First branch of the second tangential flow filter connecting pipe, 322-Second branch of the second tangential flow filter connecting pipe, 33-Third tangential flow filter connecting pipe, 331-First branch of the third tangential flow filter connecting pipe, 332-Second branch of the third tangential flow filter connecting pipe, 34-Fourth tangential flow filter connecting pipe, 341-First branch of the fourth tangential flow filter connecting pipe, 342-Second branch of the fourth tangential flow filter connecting pipe, 35-Fifth tangential flow filter connecting pipe, 36-Sixth tangential flow filter connecting pipe. Detailed Implementation

[0020] The technical solution of this utility model will be further described below with reference to the accompanying drawings to make it easier to understand and master. The various connecting pipes, sensors, and valves involved are all commonly used components recognized by those skilled in the art, and there are no special requirements for them in this application.

[0021] like Figure 1As shown, this embodiment 1 provides an integrated chromatography and tangential flow filtration device with switchable flow paths. It employs a chromatography flow path structure, which includes a first pump body 1, a second pump body 2, a column position valve assembly 11, a detection sensor assembly 13, and a chromatography column 15. A first chromatography connecting pipe 22 is connected to the first end of the first pump body 1, and several first valves 3 are connected to the first chromatography connecting pipe 22. A second chromatography connecting pipe 23 is connected to the second end of the first pump body 1. A third chromatography connecting pipe 24 is connected to the first end of the second pump body 2, and several second valves 4 are connected to the third chromatography connecting pipe 24. The second pump body 2 has a fourth chromatography connecting pipe 25 connected to its second end. The fourth chromatography connecting pipe 25 is connected to the first end of the second chromatography connecting pipe 23. The fourth chromatography connecting pipe 25 is also equipped with a flow meter 5 and a first pressure sensor 6. The second end of the fourth chromatography connecting pipe 25 is divided into a first branch pipe 251 and a second branch pipe 252. The first end of the first branch pipe 251 is connected to a bubble trap 16. The second end of the first branch pipe 251 is connected to a detection sensor group 13 and a chromatography column 15 through a column position valve group 11.

[0022] Preferably, in the above structure: the first branch pipe 251 of the fourth chromatographic connecting pipe is also connected to a fourth valve 8 and a first normally closed valve 10 (the combined flow path enters the column position valve group 11 through the first normally closed valve 10), and the first branch pipe 251 of the fourth chromatographic connecting pipe located at both ends of the fourth valve 8 is respectively connected to a tenth chromatographic connecting pipe 30, and the tenth chromatographic connecting pipe 30 is connected to the bubble trap 16 through a double row of tenth chromatographic connecting pipes 30, and a fifth valve 9 is connected to each of the tenth chromatographic connecting pipes 30; the second branch pipe 252 of the fourth chromatographic connecting pipe is connected to a third valve 7.

[0023] Preferably, in the above structure, the chromatography column 15 and the column position valve group 11 are cyclically connected through a fifth chromatography connecting pipe 26 and a sixth chromatography connecting pipe 27.

[0024] Preferably, in the above structure: the detection sensor group 13 in the chromatography flow path structure is connected to the column position valve group 11 through a seventh chromatography connecting pipe 28, and a second normally closed valve 12 is connected to the seventh chromatography connecting pipe 28; the detection sensor group 13 is also connected to an eighth chromatography connecting pipe 29, and several sixth valves 14 are connected to the eighth chromatography connecting pipe 29 for waste discharge or material discharge.

[0025] Preferably, in the above structure: the column position valve group 11 in the chromatography flow path structure is a diamond-shaped regulating valve composed of four single column position valves 111, so as to control the fluid to flow through the chromatography column 15 in the forward, reverse or bypass direction for chromatography operation as needed.

[0026] The working principle of the chromatography flow path structure: By switching to the chromatography operation software to control the equipment components, the first pump body 1 and the second pump body 2 merge through the flow path to the fourth chromatography connecting pipe 25. The fluid can be controlled to enter the bubble trap (i.e., bypass) through the fourth valve 8 and two sets of fifth valves 9, and then enter the column position valve group 11. The combination of four single column position valves 111 controls the fluid to flow through the chromatography column 15 in the forward, reverse or bypass direction for chromatography operation. After passing through the chromatography column 15, it flows out through the second normally closed valve 12. After being processed by the chromatography column 15, it is detected by a detection sensor group 13 and then discharged through the sixth valve 14 (i.e., outlet valve).

[0027] like Figure 2As shown, this embodiment 2 provides another integrated chromatography and tangential flow filtration device with switchable flow paths, employing a tangential flow filtration flow path structure. The tangential flow filtration flow path structure includes a first pump body 1, a second pump body 2, a detection sensor group 13, and a circulation tank 17. The first end of the first pump body 1 is provided with several first valves 3, and the second end of the first pump body 1 is connected to the feed end of the circulation tank 17 via a first tangential flow filtration connecting pipe 31. The first end of the second pump body 2 is connected to a second tangential flow filtration connecting pipe 32, which is divided into a first branch pipe 321 and a second branch pipe 322. The second branch pipe 322 of the tangential flow filter connecting pipe is connected to the outlet end of the circulation tank 17. A second valve 4 is connected to the first branch pipe 321 of the second tangential flow filter connecting pipe, and a second valve 4 is connected to the second branch pipe 322 of the second tangential flow filter connecting pipe. The second end of the second pump body 2 is connected to a third tangential flow filter connecting pipe 33. The third tangential flow filter connecting pipe 33 is equipped with a flow meter 5 and a first pressure sensor 6. The third tangential flow filter connecting pipe 33 is also branched into a first branch pipe 331 of the third tangential flow filter connecting pipe. The second branch pipe 332, the first branch pipe 331 of the third tangential flow filter connecting pipe is also connected to a fourth valve 8 and a first normally closed valve 10, and the first branch pipe 331 of the third tangential flow filter connecting pipe located at both ends of the fourth valve 8 is respectively connected to a sixth tangential flow filter connecting pipe 36, and the detection sensor group 13 is connected through the double rows of the sixth tangential flow filter connecting pipes 36. Each of the sixth tangential flow filter connecting pipes 36 is connected to a fifth valve 9, and a membrane 18 is provided between the detection sensor group 13 and the fifth valve 9 through the sixth tangential flow filter connecting pipe 36; the return end of the circulation tank 17 is connected to a... A fourth tangential flow filter connecting pipe 34 is provided, on which a metering control valve 19, a second conductivity sensor 20, and a third pressure sensor 21 are sequentially connected. The fourth tangential flow filter connecting pipe 34 is further divided into a first branch pipe 341 and a second branch pipe 342. The first branch pipe 341 is connected to the detection sensor group 13, and the second branch pipe 342 is connected to the first branch pipe 331 of the third tangential flow filter connecting pipe, for circulating connection between the second pump body 2 and the circulation tank 17.

[0028] Preferably, in the above structure: a single-position valve 111 is connected to the first branch pipe 341 of the fourth tangential flow filter connecting pipe and the second branch pipe 342 of the fourth tangential flow filter connecting pipe, so as to control the fluid flow of different pipes through the single-position valve 111.

[0029] Preferably, in the above structure: the detection sensor group 13 in the tangential flow filter path structure is connected to the first branch pipe 341 of the fourth tangential flow filter connecting pipe through the fifth tangential flow filter connecting pipe 35, and a sixth valve 14 is connected to the fifth tangential flow filter connecting pipe 35.

[0030] Specifically, in the structure of the above-mentioned switchable flow path integrated chromatography and tangential flow filtration device, the detection sensor group 13 includes several first conductivity sensors 131, ultraviolet sensors 132, pH sensors 133 and second pressure sensors 134, so as to detect fluid parameters for drug quality monitoring and regulation.

[0031] The working principle of the tangential flow filtration flow path structure: By switching to the tangential flow filtration operation software for equipment component control, the first pump body 1 (i.e., the replenishment pump) selectively replenishes fluid into the circulation tank 17 through multiple first valves 3 (i.e., inlet valves); the second pump body 2 (i.e., the feed pump) passes the fluid through the flow meter 5 and the first pressure sensor 6, and then the fourth valve 8 and two sets of fifth valves 9 control the fluid to enter the membrane envelope 18 (or bypass). The first normally closed valve 10 and a single-position valve 111 control the fluid to flow back to the circulation tank 17, and the circulation tank 17 then refluxes the fluid. The metering control valve 19 at the end precisely controls and regulates the pressure of the fluid to ensure that the system pressure is maintained within the set range. The flow path at the outlet of the circulation tank 17 is detected by the detection sensor group 13 and discharged to the sixth valve 14 at the outlet. Alternatively, it can be circulated back to the circulation tank 17 through the single-position valve 111 set on the first branch pipe 341 of the fourth tangential flow filter connecting pipe. The fluid flow path can be opened and closed by the single-position valve 111 (or another standby single-position valve 111) set on the first branch pipe 331 of the third tangential flow filter connecting pipe to help the membrane 18 perform integrity testing.

[0032] The key technical aspect of this invention lies in its switchable flow path structure for chromatography and its tangential flow filtration structure. Figure 1 The focus is on showcasing the piping components and specific flow path structure involved in the chromatography flow path. Figure 2 The focus is on showcasing the piping components and specific structure of the tangential flow filtration system. By altering the size, quantity, and arrangement of the piping, valves, and sensors, the system achieves switching between chromatographic and flow filtration structures, enabling dual-purpose operation and shared basic components. For the various connecting pipes, sensors, and valves required for the flow path, those skilled in the art can perform conventional setups based on existing technology; this application does not have special requirements regarding model selection or combination.

[0033] As can be seen from the above description, compared with the prior art, after adopting the technical solution of this utility model, the chromatography and tangential flow filtration systems are integrated into the same device. By switching the flow path structure, the process development work that originally required two devices can be completed, realizing dual-purpose use of one machine, improving the utilization rate of the device, saving resources and reducing waste, and effectively saving experimental space. Moreover, the types and quantities of flow pipelines, valves and detection sensors can be changed according to the process development requirements of chromatography or tangential flow filtration, thereby adjusting the upper limit value of the working flow rate. This can meet various application scenarios from small-scale development to pilot-scale amplification, maximizing the working range and application scenarios of the device. Furthermore, the replaced flow pipelines, valves or detection sensors are all disposable flow path consumables that can be directly discarded after use, so as to save cleaning time, manpower and verification costs in pilot production.

[0034] The technical solution, working process and implementation effect of this utility model have been described in detail above. It should be noted that the described example is only a typical example of this utility model. In addition, this utility model may have many other specific implementation methods. All technical solutions formed by equivalent substitution or equivalent transformation fall within the scope of protection claimed by this utility model.

Claims

1. A switchable flow path integrated chromatography and tangential flow filtration device, comprising a chromatography flow path structure and a tangential flow filtration flow path structure, characterized in that: The chromatography flow path structure includes a first pump body (1), a second pump body (2), a column position valve group (11), a detection sensor group (13), and a chromatography column (15). The first pump body (1) is connected to a first chromatography connecting pipe (22) at its first end, and several first valves (3) are connected to the first chromatography connecting pipe (22). The second pump body (1) is connected to a second chromatography connecting pipe (23) at its second end. The second pump body (2) is connected to a third chromatography connecting pipe (24) at its first end, and several second valves (4) are connected to the third chromatography connecting pipe (24). The second pump body (2) is connected to a fourth chromatography connecting pipe at its second end. The fourth chromatography connecting tube (25) is connected to the first end of the second chromatography connecting tube (23). The fourth chromatography connecting tube (25) is also equipped with a flow meter (5) and a first pressure sensor (6). The second end of the fourth chromatography connecting tube (25) is divided into a first branch tube (251) and a second branch tube (252). The first end of the first branch tube (251) is connected to a bubble trap (16). The second end of the first branch tube (251) is connected to a detection sensor group (13) and a chromatography column (15) through a column position valve group (11).

2. The integrated chromatography and tangential flow filtration device with switchable flow paths according to claim 1, characterized in that: The tangential flow filtration path structure includes a first pump body (1), a second pump body (2), a detection sensor group (13), and a circulation tank (17). The first pump body (1) has several first valves (3) at its first end, and the second end of the first pump body (1) is connected to the feed end of the circulation tank (17) through a first tangential flow filtration connecting pipe (31). The first end of the second pump body (2) is connected to a second tangential flow filtration connecting pipe (32), which is divided into a first branch pipe (321) and a second branch pipe (322). The first branch pipe (321) of the connecting pipe is connected to the outlet end of the circulating tank (17), and the second branch pipe (322) of the second tangential flow filter connecting pipe is connected to a second valve (4); the second end of the second pump body (2) is connected to a third tangential flow filter connecting pipe (33), the third tangential flow filter connecting pipe (33) is equipped with a flow meter (5) and a first pressure sensor (6), and the third tangential flow filter connecting pipe (33) is divided into a first branch pipe (331) and a second branch pipe (332), and the first branch pipe (331) of the third tangential flow filter connecting pipe is also connected to a fourth valve (4). 8) The first branch pipe (331) of the third tangential flow filter connecting pipe, which is connected to the first normally closed valve (10) and located at both ends of the fourth valve (8), is respectively provided with a sixth tangential flow filter connecting pipe (36). The detection sensor group (13) is connected through the double row of the sixth tangential flow filter connecting pipes (36). A fifth valve (9) is connected to each of the sixth tangential flow filter connecting pipes (36). A membrane (18) is provided between the detection sensor group (13) and the fifth valve (9) through the sixth tangential flow filter connecting pipe (36). The return end of the circulation tank (17) is connected with a fourth tangential flow filter connecting pipe (34). A metering control valve (19), a second conductivity sensor (20), and a third pressure sensor (21) are sequentially connected to the fourth tangential flow filter connecting pipe (34). The fourth tangential flow filter connecting pipe (34) is divided into a first branch pipe (341) and a second branch pipe (342). The first branch pipe (341) is connected to the detection sensor group (13), and the second branch pipe (342) is connected to the first branch pipe (331) of the third tangential flow filter connecting pipe, so as to circulate the second pump body (2) and the circulating tank (17).

3. The integrated chromatography and tangential flow filtration device with switchable flow paths according to claim 1, characterized in that: The first branch pipe (251) of the fourth chromatographic connecting pipe is also connected to a fourth valve (8) and a first normally closed valve (10). The first branch pipe (251) of the fourth chromatographic connecting pipe located at both ends of the fourth valve (8) is connected to a tenth chromatographic connecting pipe (30). The tenth chromatographic connecting pipe (30) is connected to the bubble trap (16) through a double row of tenth chromatographic connecting pipes (30). Each tenth chromatographic connecting pipe (30) is connected to a fifth valve (9). The second branch pipe (252) of the fourth chromatographic connecting pipe is connected to a third valve (7).

4. The integrated chromatography and tangential flow filtration device with switchable flow paths according to claim 1, characterized in that: The chromatography column (15) and the column position valve group (11) are connected in a loop through the fifth chromatography connecting pipe (26) and the sixth chromatography connecting pipe (27).

5. The integrated chromatography and tangential flow filtration device with switchable flow paths according to claim 1, characterized in that: The detection sensor group (13) in the chromatography flow path structure is connected to the column position valve group (11) through a seventh chromatography connecting pipe (28), and a second normally closed valve (12) is connected to the seventh chromatography connecting pipe (28).

6. The integrated chromatography and tangential flow filtration device with switchable flow paths according to claim 5, characterized in that: The detection sensor group (13) in the chromatography flow path structure is also connected to an eighth chromatography connecting pipe (29), and several sixth valves (14) are connected to the eighth chromatography connecting pipe (29).

7. The integrated chromatography and tangential flow filtration device with switchable flow paths according to claim 2, characterized in that: A single-position valve (111) is connected to the first branch (341) and the second branch (342) of the fourth tangential flow filter connecting pipe, respectively.

8. The integrated chromatography and tangential flow filtration device with switchable flow paths according to claim 2, characterized in that: The detection sensor group (13) in the tangential flow filter path structure is connected to the first branch pipe (341) of the fourth tangential flow filter connecting pipe through the fifth tangential flow filter connecting pipe (35), and a sixth valve (14) is connected to the fifth tangential flow filter connecting pipe (35).

9. The integrated chromatography and tangential flow filtration device with switchable flow paths according to claim 1, characterized in that: The column valve group (11) in the chromatography flow path structure is a diamond-shaped regulating valve composed of four single column valves (111).

10. The integrated chromatography and tangential flow filtration apparatus with switchable flow paths according to claim 1 or 2, characterized in that: The detection sensor group (13) includes several first conductivity sensors (131), ultraviolet sensors (132), pH sensors (133) and second pressure sensors (134).

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