Film sealing device

By designing a sealing device with silicone strips and fine sealing teeth, the problem of incomplete sealing of 96-well PCR plates was solved, achieving efficient and reliable sealing and ensuring the accuracy and efficiency of experimental results.

CN223618980UActive Publication Date: 2025-12-02CHONGQING JINYU MEDICAL LAB CO LTD
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Patent Information

Application Number
CN202520023351.8
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2025-01-06
Publication Date
2025-12-02
Estimated Expiration
2035-01-06

AI Technical Summary

Technical Problem

In existing technologies, the sealing film of 96-well PCR plates is not tightly sealed, which leads to the volatilization of PCR products or contamination between wells, which is time-consuming and labor-intensive and affects the experimental results.

Method used

A sealing device was designed, which uses a silicone strip and a sealing tooth structure to ensure that the sealing film fits tightly with the PCR plate. The sealing operation is achieved through a movable block and spring mechanism, including the prying part to pry open the four corners and edges of the sealing film.

Benefits of technology

It improves the sealing performance, prevents PCR product volatilization and contamination, reduces the risk of experimental failure, improves experimental efficiency and success rate, and avoids errors caused by repetitive work and fatigue.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model discloses a film sealing device, which relates to the technical field of laboratory detection and comprises a film sealing device main body, a fixing strip is fixedly connected to the front side of the outer surface of the film sealing device main body, a silica gel strip is fixedly connected to the front side surface of the fixing strip, and sealing fine teeth are fixedly connected to the outer surface of the film sealing device main body and correspond to the fixing strip. An extending opening is formed in the tail end of the left side of the film sealing device body in a penetrating mode, a movable block is slidably connected to the interior of the film sealing device body, an elastic piece is fixedly connected to one side of the movable block, an inclined film prying part is arranged at the tail end of the elastic piece, and an extrusion spring is fixedly connected to the side, away from the elastic piece, of the movable block. A clamping groove is formed in the side, close to the sleeve, of the movable block. The PCR plate sealing device has the advantages that the sealing film and the PCR plate are ensured to be tightly attached by using the film sealing device, and a PCR product is effectively prevented from being volatilized in an experiment process by the tight attachment, so that the accuracy and the reliability of an experiment result are ensured.
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Description

Technical Field

[0001] This utility model belongs to the field of laboratory testing, and specifically relates to a sealing device. Background Technology

[0002] 96-well PCR plates are reaction plates used to hold chemical reagents for detecting chemical components, drug residues, virus types, etc. They are generally used by hospitals and pharmaceutical companies to obtain corresponding results after experiments to judge the effectiveness of chemical reagents.

[0003] In molecular laboratory testing for medical applications, nucleic acids extracted from samples are often placed into 96-well PCR plates and then into PCR instruments for subsequent amplification experiments. Because PCR instruments involve a high-temperature denaturation step, reaching up to 98°C, each well of the 96-well PCR plate containing the nucleic acid must be sealed tightly with a special plastic sealing film before amplification.

[0004] Currently, the most common sealing method is to press the sealing film tightly to the tube opening. However, the sealing film is often not airtight, which can lead to PCR product volatilization or contamination between wells and poor experimental results. Furthermore, because PCR experiments have high requirements for airtight tube openings, repeated pressing is required, which is time-consuming and labor-intensive. Summary of the Invention

[0005] The purpose of this invention is to provide a sealing device that provides a tight seal, prevents product evaporation and contamination due to an unsealed film, improves efficiency, and saves time and effort.

[0006] The technical solution is as follows:

[0007] A sealing device includes a sealing device body. A fixing strip is fixedly connected to the front side of the outer surface of the sealing device body. A silicone strip is fixedly connected to the front surface of the fixing strip. Sealing teeth are fixedly connected to the outer surface of the sealing device body at positions corresponding to the fixing strip. An extension opening is provided through the left end of the sealing device body. A movable block is slidably connected inside the sealing device body. A spring is fixedly connected to one side of the movable block. An inclined prying part is provided at the end of the spring. A compression spring is fixedly connected to the side of the movable block away from the spring. A sleeve is fixedly connected to the right side of the upper surface of the sealing device body. A slot is provided on the side of the movable block near the sleeve.

[0008] In one embodiment, a movable rod is slidably connected inside the sleeve, and a toggle piece is fixedly connected to the top of the movable rod and the outside of the sleeve.

[0009] In one embodiment, a locking block is fixedly connected to one end of the movable rod near the locking slot, and a return spring is sleeved on the outside of the movable rod. One end of the return spring is fixedly connected to the locking block, and the other end of the return spring is fixedly connected to the top wall of the sleeve.

[0010] In one embodiment, a handle is fixedly connected to the top of the upper surface of the sealing device body.

[0011] In one embodiment, a limiting ring for blocking is fixedly connected inside the main body of the sealing device to the left side of the movable block.

[0012] In one embodiment, a sealing cap is threaded to the right end of the sealing device body, a limiting post is fixedly connected to the inner side of the sealing cap, and the end of the limiting post extends into the compression spring.

[0013] In one embodiment, the spring and the skid film part are an integral structure, and the axes of the spring and the skid film part are collinear with the axis of the sealing device body.

[0014] In one embodiment, the cross-section of the sealing device body and the cross-section of the movable block are both circular.

[0015] In one embodiment, the number of sealing teeth is at least thirteen.

[0016] In one embodiment, the inner side of the handle is provided with an arc-shaped groove.

[0017] The technical solution provided by this utility model has the following advantages and effects:

[0018] 1. The silicone strip and sealing teeth ensure a tight seal between the sealing film and the PCR plate. This tight seal effectively prevents the volatilization of PCR products during the experiment, thus guaranteeing the accuracy and reliability of the experimental results. Its highly efficient sealing performance effectively isolates external contaminants, reducing the risk of experimental contamination. This not only improves the success rate of the experiment but also reduces the time and resource waste caused by experimental failures. After the amplification experiment is completed, the four corners and edges of the sealing film can be easily pried open using the PCR prying mechanism.

[0019] 2. By using a sealing device, multiple samples can be sealed at once or in batches, while ensuring that each seal achieves the same tightness. This avoids repetitive work and errors caused by fatigue, improving efficiency and saving time and effort. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the structure of an embodiment of the present utility model;

[0021] Figure 2 This is a top view of an embodiment of the present utility model;

[0022] Figure 3 for Figure 2 Sectional view at point AA;

[0023] Figure 4 for Figure 3 A magnified view of a portion of point A in the middle.

[0024] Explanation of reference numerals in the attached figures:

[0025] 1. Sealing device body; 2. Fixing strip; 3. Silicone strip; 4. Outlet; 5. Sealing teeth; 6. Handle; 7. Sealing cover; 8. Sleeve; 9. Movable rod; 10. Actuating piece; 11. Movable block; 12. Slot; 13. Spring piece; 14. Film prying part; 15. Limiting ring; 16. Compression spring; 17. Limiting post; 18. Return spring; 19. Locking block. Detailed Implementation

[0026] To facilitate understanding of this utility model, the specific embodiments of this utility model will be described in more detail below with reference to the accompanying drawings.

[0027] Unless otherwise specified or defined, the terms "first," "second," etc., used in this document are for distinguishing names only and do not represent a specific number or order.

[0028] Unless otherwise stated or defined, the term “and / or” as used herein includes any and all combinations of one or more of the associated listed items.

[0029] It should be noted that in this article, "fixed to" or "connected to" can mean directly fixed to or connected to a component, or indirectly fixed to or connected to a component.

[0030] like Figures 1-4The sealing device shown includes a sealing device body 1. A fixing strip 2 is fixedly connected to the front side of the outer surface of the sealing device body 1. A silicone strip 3 is fixedly connected to the front surface of the fixing strip 2. The fixing strip 2 firmly fixes the silicone strip 3 to the sealing device body 1. The silicone strip 3 has excellent flexibility and sealing properties, which can tightly fit the sealing film, effectively expel air, and improve the sealing effect. Sealing teeth 5 are fixedly connected to the outer surface of the sealing device body 1 at the corresponding position of the fixing strip 2. The design of the sealing teeth 5 enhances the friction between the sealing device body 1 and the sealing film, so that the sealing film can fit more tightly on the 96-well PCR plate, preventing air and contaminants from entering. The sealing and stability of the sealing film are improved. An extension 4 is provided through the left end of the sealing device body 1. The extension 4 provides space for the prying part 14 to extend, so that the prying part 14 can easily pry open the four corners and edges of the sealing film, which is convenient for the user to carry out subsequent operations. A movable block 11 is slidably connected inside the sealing device body 1. A spring piece 13 is fixedly connected to one side of the movable block 11. An inclined prying part 14 is provided at the end of the spring piece 13. A compression spring 16 is fixedly connected to the side of the movable block 11 away from the spring piece 13. A sleeve 8 is fixedly connected to the right side of the upper surface of the sealing device body 1. A slot 12 is provided on the side of the movable block 11 near the sleeve 8.

[0031] Please refer to Figure 4 As shown, a movable rod 9 is slidably connected inside the sleeve 8. A toggle piece 10 is fixedly connected to the top of the movable rod 9 and the outside of the sleeve 8. The movable rod 9 can easily control the raising and lowering of the locking block 19 through the toggle piece 10, thereby locking and unlocking the movable block 11. This design allows users to easily operate the sealing device, improving ease of use.

[0032] Please refer to Figure 4 As shown, a locking block 19 is fixedly connected to one end of the movable rod 9 near the locking slot 12. A return spring 18 is sleeved on the outside of the movable rod 9. One end of the return spring 18 is fixedly connected to the locking block 19, and the other end of the return spring 18 is fixedly connected to the top wall of the sleeve 8. The cooperation between the locking block 19 and the locking slot 12 realizes the locking function of the movable block 11, preventing the movable block 11 from moving on its own when it is not subjected to external force.

[0033] Please refer to Figure 3 As shown, a handle 6 is fixedly connected to the top of the upper surface of the sealing device body 1.

[0034] Please refer to Figure 3 As shown, a limiting ring 15 for blocking is fixedly connected inside the main body 1 of the sealing device and the left side of the movable block 11. The design of the limiting ring 15 prevents the movable block 11 from shifting excessively during movement and prevents the prying part 14 from extending excessively.

[0035] Please refer to Figure 3As shown, a sealing cover 7 is threadedly connected to the right end of the sealing device body 1. A limiting post 17 is fixedly connected to the inner side of the sealing cover 7, and the end of the limiting post 17 extends into the inside of the compression spring 16. The sealing cover 7 is threadedly connected to the right end of the sealing device body 1, which can effectively prevent dust and contaminants from entering the sealing device, and at the same time facilitate the maintenance of the sealing device. The limiting post 17 can prevent the compression spring 16 from shifting during movement.

[0036] Please refer to Figure 3 As shown, the spring piece 13 and the film-prying part 14 are an integral structure, and the axes of the spring piece 13 and the film-prying part 14 are collinear with the axis of the sealing device body 1. The integral structure of the spring piece 13 and the film-prying part 14 ensures the stability and reliability of the structure. The inclined design of the film-prying part 14 allows it to pry open the sealing film more effectively.

[0037] Please refer to Figure 1 As shown, the cross-section of the main body 1 of the sealing device and the cross-section of the movable block 11 are both circular.

[0038] Please refer to Figure 1 and Figure 2 As shown, the number of sealing teeth 5 is at least thirteen.

[0039] Please refer to Figure 3 As shown, the inner side of the handle 6 is provided with an arc-shaped groove that conforms to the shape of the fingers, which allows the user to hold the sealing device more comfortably during use.

[0040] Working principle: When using this device, first attach the sealing film to the surface of the 96-well PCR plate. Then, hold the handle 6 so that the silicone strip 3 contacts the sealing film. Apply downward force to push the sealing film to expel the air. After the air is expelled, reverse the device and use the sealing teeth 5 on the other side of the sealing device body 1 to align with the wells of the 96-well PCR plate and slide it down from top to bottom. Then rotate the 96-well PCR plate 90 degrees and continue to slide it down from top to bottom. This will quickly and efficiently seal the film to the wells. When it is necessary to tear the film, pull the lever 10 upward with your finger so that the movable rod 9 pulls the locking block 19 upward, causing the locking block 19 to disengage from the slot 12. Then, squeeze the spring 16 to restore its elasticity and push the movable block 11 to the left, so that the prying part 14 extends out of the outlet 4. The prying part 14 can be used to pry open the four corners and edges of the sealing film.

[0041] The above embodiments are not an exhaustive list based on the present invention, and there may be other embodiments not listed. Any substitutions and improvements made without departing from the concept of the present invention are within the protection scope of the present invention.

Claims

1. A sealing device, characterized in that, The device includes a sealing device body. A fixing strip is fixedly connected to the front side of the outer surface of the sealing device body. A silicone strip is fixedly connected to the front surface of the fixing strip. Sealing teeth are fixedly connected to the outer surface of the sealing device body at positions corresponding to the fixing strip. An extension opening is provided through the left end of the sealing device body. A movable block is slidably connected inside the sealing device body. A spring is fixedly connected to one side of the movable block. An inclined prying part is provided at the end of the spring. A compression spring is fixedly connected to the side of the movable block away from the spring. A sleeve is fixedly connected to the right side of the upper surface of the sealing device body. A slot is provided on the side of the movable block near the sleeve.

2. The sealing device as described in claim 1, characterized in that: A movable rod is slidably connected inside the sleeve, and a toggle piece is fixedly connected to the top of the movable rod and the outside of the sleeve.

3. The sealing device as described in claim 2, characterized in that: A locking block is fixedly connected to one end of the movable rod near the locking slot. A return spring is sleeved on the outside of the movable rod. One end of the return spring is fixedly connected to the locking block, and the other end of the return spring is fixedly connected to the top wall of the sleeve.

4. The sealing device as described in claim 1, characterized in that: A handle is fixedly connected to the top of the upper surface of the sealing device.

5. The sealing device as described in claim 1, characterized in that: A limiting ring for blocking is fixedly connected inside the main body of the sealing device and to the left side of the movable block.

6. The sealing device as described in any one of claims 1 to 5, characterized in that: The sealing device has a sealing cap threaded to the right end of its main body. A limiting post is fixedly connected to the inside of the sealing cap, and the end of the limiting post extends into the compression spring.

7. The sealing device as claimed in claim 1, characterized in that: The spring sheet and the skid film part are an integral structure, and the axes of the spring sheet and the skid film part are collinear with the axis of the main body of the sealing device.

8. The sealing device as claimed in claim 1, characterized in that: The cross-section of the main body of the sealing device and the cross-section of the movable block are both circular.

9. The sealing device as claimed in claim 1, characterized in that: The number of sealing teeth is at least thirteen.

10. The sealing device as described in claim 4, characterized in that: The handle has an arc-shaped groove on its inner side.