NK cell extraction device

Abstract

The utility model discloses an NK (Natural Killer) cell extraction device, and aims to provide an NK cell extraction device which is simple and convenient to operate and good in use effect. Comprising a mounting base, an extraction tube for containing an extracting solution is mounted in the mounting base, the extraction tube is filled with a plurality of magnetic separation beads, the surfaces of the magnetic separation beads are coated with extracted NK cell antibodies, an electromagnet is arranged on the outer side of the mounting base, a rotatable suction cup is mounted at the bottom in the mounting base, and the suction cup is connected with the extraction tube. The device disclosed by the utility model has the beneficial effects that the device is simple and convenient to operate during extraction operation; the NK cells are extracted through centrifugal operation in cooperation with magnetic separation beads, and the use effect is good.

Description

Technical Field

[0001] This utility model relates to the field of cell extraction technology, and in particular to an NK cell extraction device. Background Technology

[0002] NK cell (natural killer cell) extraction devices are used to isolate and extract NK cells from human peripheral blood, umbilical cord blood, or other tissue sources. NK cells are important immune cells in the body, playing a crucial role in anti-tumor, anti-viral infection, and immune regulation. Obtaining high-purity, highly active NK cells through this extraction device can provide a foundation for fields such as cell immunotherapy.

[0003] Existing NK cell extraction devices are complex to operate and have poor performance. Therefore, we need to improve the existing devices to solve the above problems. Utility Model Content

[0004] The present invention aims to overcome the shortcomings of existing technologies, such as inconvenient operation and poor performance, and provides an NK cell extraction device that is simple and convenient to operate and has good performance.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: an NK cell extraction device, comprising a mounting base, an extraction tube containing an extraction solution installed inside the mounting base, a plurality of magnetic beads filled inside the extraction tube, the surface of the magnetic beads being coated with the NK cell antibodies to be extracted, an electromagnet provided on the outside of the mounting base, and a rotatable suction cup installed at the bottom inside the mounting base, the suction cup being connected to the extraction tube.

[0006] This extraction device includes a mounting base inside which an extraction tube is installed, ensuring the tube remains vertically stable. The extraction tube is filled with several magnetic beads coated with NK cell antibodies, used to extract target NK cells. An electromagnet is located on the outside of the mounting base; when energized, it generates a magnetic attraction force that draws the magnetic beads to the tube wall, facilitating NK cell extraction. Simultaneously, a rotatable suction cup is installed at the bottom inner side of the mounting base. This suction cup holds the extraction tube in place, and its rotation also drives the tube to rotate, performing centrifugation and enhancing the adsorption of NK cells by the magnetic beads, thus improving the extraction efficiency. This device is simple, convenient, and effective.

[0007] Preferably, the mounting base has a mounting groove on its top, a stabilizing ring is mounted on the side wall of the mounting groove, and several stabilizing balls are mounted on the inner side wall of the stabilizing ring. The stabilizing balls contact the extraction tube. The mounting groove inside the mounting base is used to place the extraction tube. Simultaneously, the stabilizing ring mounted on the side wall of the mounting groove, with several stabilizing balls on its inner wall, contacts the extraction tube. This allows the stabilizing balls to stabilize the extraction tube during rotation, ensuring better centrifugation and improving the overall performance of the device.

[0008] Preferably, the extraction tube is provided with a valve-shaped sealing cap at the top. This valve-shaped sealing cap facilitates the insertion of the reagent extraction gun, allows for easy aspiration and injection of the extract into the tube, and ensures a tight seal during centrifugation to prevent leakage. This simplifies operation and improves performance.

[0009] Preferably, the bottom of the mounting groove is provided with a sinking groove, and a rotating motor is connected to the bottom of the sinking groove. The rotating motor is connected to the suction cup. The rotation of the rotating motor can drive the suction cup to rotate, which in turn drives the extraction tube to rotate, realizing the centrifugation operation. This structural design facilitates the centrifugation operation of the extraction tube, making the operation simple and convenient.

[0010] Preferably, the suction cup is arc-shaped, and an suction hole is provided at the inner bottom of the suction cup, which is connected to a vacuum pump. The arc shape of the suction cup allows it to adhere to the extraction tube. Through the suction hole at the inner bottom of the suction cup, and in conjunction with the connected vacuum pump, the suction cup can stably hold the extraction tube, ensuring that the rotation of the suction cup can drive the extraction tube to rotate, achieving centrifugation and ensuring good performance of the device.

[0011] Preferably, the suction cup is connected to a suction cup rod, and a telescopic rod is installed at the bottom of the suction cup rod, which is connected to a rotating motor. The suction cup rod at the bottom of the suction cup, with its telescopic rod, ensures stable contact with the bottom of the extraction tube through its extension and retraction, guaranteeing tight and stable contact with the extraction tube during vacuum suction, thus achieving good performance of the device.

[0012] The beneficial effects of this invention are: the device is simple and convenient to operate during extraction; the extraction of NK cells is achieved through centrifugation in conjunction with magnetic beads, resulting in good efficacy. Attached Figure Description

[0013] Figure 1 This is a sectional view of the present invention;

[0014] Figure 2This is a top view of the sealing cover of this utility model.

[0015] In the attached diagram: 1. Mounting base, 2. Extraction tube, 3. Magnetic bead, 4. Electromagnet, 5. Suction cup, 10. Mounting groove, 11. Stabilizing ring, 12. Stabilizing ball, 13. Sinking groove, 14. Rotating motor, 20. Sealing cap, 50. Suction hole, 51. Suction cup rod, 52. Telescopic rod. Detailed Implementation

[0016] The present invention will be further described below with reference to the accompanying drawings and specific embodiments. Obviously, the described embodiments are only a part of the embodiments of this application, and not all of them. The following description of at least one exemplary embodiment is merely illustrative and is in no way intended to limit the present application or its application or use. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

[0017] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments according to this application. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0018] Furthermore, it should be noted that the use of terms such as "first" and "second" to define components is merely for the purpose of distinguishing the corresponding components. Unless otherwise stated, the above terms have no special meaning and therefore cannot be construed as limiting the scope of protection of this application.

[0019] Example 1, such as Figure 1-2 As shown, an NK cell extraction device includes a mounting base 1, an extraction tube 2 containing extraction liquid installed inside the mounting base 1, a plurality of magnetic beads 3 filled inside the extraction tube 2, the surface of the magnetic beads 3 being coated with the NK cell antibodies to be extracted, an electromagnet 4 disposed on the outside of the mounting base 1, and a rotatable suction cup 5 installed at the bottom of the mounting base 1, the suction cup 5 being connected to the extraction tube 2.

[0020] The mounting base 1 has a mounting groove 10 on its top. A stabilizing ring 11 is installed on the side wall of the mounting groove 10. A plurality of stabilizing balls 12 are installed on the inner side wall of the stabilizing ring 11. The stabilizing balls 12 are in contact with the extraction tube 2.

[0021] The top of the extraction tube 2 is provided with a valve-shaped sealing cap 20.

[0022] The bottom of the mounting slot 10 is provided with a sinking groove 13, and the bottom of the sinking groove 13 is connected to a rotating motor 14, which is connected to the suction cup 5.

[0023] The suction cup 5 is arc-shaped, and the bottom of the suction cup 5 is provided with a suction hole 50, which is connected to a vacuum pump.

[0024] The suction cup 5 is connected to a suction cup rod 51, and a telescopic rod 52 is installed at the bottom of the suction cup rod 51. The telescopic rod 52 is connected to the rotating motor 14.

[0025] The working principle of this utility model is as follows: Figure 1-2 As shown, this extraction device includes a mounting base 1, inside which an extraction tube 2 is installed. The mounting base 1 ensures that the extraction tube 2 is in a vertical and stable state. Several magnetic beads 3 are filled inside the extraction tube 2, and these beads are coated with NK cell antibodies. The magnetic beads 3 are used to extract target NK cells. An electromagnet 4 is installed on the outside of the mounting base 1. When energized, the electromagnet 4 generates a magnetic attraction force, which attracts the magnetic beads 3 to the wall of the extraction tube 2, facilitating the extraction of NK cells. Simultaneously, a suction cup 5 is installed at the bottom inner side of the mounting base 1. This suction cup 5 is rotatable and can hold the extraction tube 2. The rotation of the suction cup 5 also drives the extraction tube 2 to rotate, performing a centrifugation operation, which improves the adsorption effect of the magnetic beads 3 on NK cells, thereby improving the extraction effect. This device is simple and convenient to operate, and has good results.

[0026] The mounting slot 10 inside the mounting base 1 is used to place the extraction tube 2. At the same time, a stabilizing ring 11 is installed on the side wall of the mounting slot 10. Several stabilizing balls 12 are provided on the inner wall of the stabilizing ring 11. The stabilizing balls 12 are in contact with the extraction tube 2, so that when the extraction tube 2 rotates, the stabilizing balls 12 play a role in stabilizing the extraction tube 2, ensuring that it can perform centrifugation better and improving the use effect of the device.

[0027] The sealing cap 20 at the top of the extraction tube 2 is valve-shaped. This structure facilitates the insertion of the reagent extraction gun, makes it easy to aspirate and inject the extract into the extraction tube 2, and also ensures a seal during centrifugation to prevent leakage. This makes the operation simple and the results good.

[0028] A sinking trough 13 is provided at the bottom of the mounting trough 10. A rotating motor 14 is connected to the bottom of the sinking trough 13. The rotating motor 14 is connected to the suction cup 5. The rotation of the rotating motor 14 can drive the suction cup 5 to rotate, which in turn can drive the extraction tube 2 to rotate, thus realizing the centrifugation operation. This structural design makes it convenient for the extraction tube 2 to be centrifuged, making the operation simple and convenient.

[0029] The suction cup 5 is arc-shaped and can adsorb the extraction tube 2. Through the suction hole 50 set at the bottom of the suction cup 5, in conjunction with the connected vacuum pump, it can ensure that the suction cup 5 stably holds the extraction tube 2, and ensure that the rotation of the suction cup 5 can drive the extraction tube 2 to rotate, realize the centrifugation operation, and ensure that the device has a good working effect.

[0030] The suction cup 5 is equipped with a suction cup rod 51 at its bottom. The telescopic rod 52 of the suction cup rod 51 can ensure stable contact with the bottom of the extraction tube 2 through telescopic movement. This ensures that the suction cup 5 can make tight and stable contact with the extraction tube 2 when vacuum suction is performed, thereby achieving good performance of the device.

[0031] The above embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit it. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this utility model.

Claims

1. An NK cell extraction device, characterized in that, The device includes a mounting base (1), an extraction tube (2) containing the extraction solution is installed inside the mounting base (1), the extraction tube (2) is filled with a number of magnetic beads (3), the surface of the magnetic beads (3) is coated with the extracted NK cell antibodies, an electromagnet (4) is provided on the outside of the mounting base (1), and a rotatable suction cup (5) is installed at the bottom inside the mounting base (1), the suction cup (5) is connected to the extraction tube (2).

2. The NK cell extraction device according to claim 1, characterized in that, The mounting base (1) has a mounting groove (10) on its top. A stabilizing ring (11) is installed on the side wall of the mounting groove (10). A number of stabilizing balls (12) are installed on the inner side wall of the stabilizing ring (11). The stabilizing balls (12) are in contact with the extraction tube (2).

3. The NK cell extraction device according to claim 1, characterized in that, The top of the extraction tube (2) is provided with a valve-shaped sealing cap (20).

4. The NK cell extraction device according to claim 2, characterized in that, The bottom of the mounting groove (10) is provided with a sinking groove (13), and the bottom of the sinking groove (13) is connected to a rotating motor (14), which is connected to a suction cup (5).

5. The NK cell extraction device according to claim 1, characterized in that, The suction cup (5) is arc-shaped, and the bottom of the suction cup (5) is provided with a suction hole (50), which is connected to a vacuum pump.

6. The NK cell extraction device according to claim 1, characterized in that, The suction cup (5) is connected to a suction cup rod (51), and a telescopic rod (52) is installed at the bottom of the suction cup rod (51). The telescopic rod (52) is connected to a rotating motor (14).

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