Sealed hydrogen peroxide detection device for cells
By introducing a mechanical structure of a movable rod and electrode plate into a sealed hydrogen peroxide detection device for cells, combined with a vacuum pump and pressure sensor, the automatic closing of the sealing door and the automatic detection of the sealing effect are realized. This solves the problems of continuous power consumption by the motor and untimely sealing detection in the prior art, and improves the automation and accuracy of the detection.
Patent Information
- Application Number
- CN202520399972.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-07
- Publication Date
- 2025-12-02
- Estimated Expiration
- 2035-03-07
AI Technical Summary
In existing sealed hydrogen peroxide detection devices for cells, the drive motor cannot automatically shut off after the sealing door is closed, continuously consuming electrical energy. The vacuum pump cannot automatically open to check the sealing effect, affecting the sealing performance of the detection chamber and the accuracy of the detection.
A sealed detection device for hydrogen peroxide in cells was designed. The device uses a mechanical structure of movable rod and electrode plate to achieve automatic closing of the sealed door. Combined with a vacuum pump and pressure sensor, the sealing effect is automatically detected to ensure the airtightness of the detection box. The control panel controls the coordinated operation of each component.
The system achieves automated control of the sealing door, reduces power consumption, improves the sealing performance and accuracy of the testing chamber, and ensures the stability and precision of the test results.
Smart Images
Figure CN223624123U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of detection device technology, specifically a sealed detection device for hydrogen peroxide in cells. Background Technology
[0002] As a key member of reactive oxygen species, cellular hydrogen peroxide is used in cell physiology research. Changes in cellular hydrogen peroxide levels can reflect the state of cell metabolism and signal transduction. Detecting it helps to understand the state of cellular energy metabolism, so a detection device is needed.
[0003] One type of sealed detection device for hydrogen peroxide in cells in the prior art often uses the principle of fluorescence detection. First, cells and fluorescent probes are placed in a sealed reaction chamber. After the cells produce hydrogen peroxide, they react with the probes and change their fluorescence properties, exciting a light source to emit light of a specific wavelength. The light shines through the reaction chamber onto the sample, causing the fluorescent probes to emit fluorescence. A detection camera and a fluorescence sensor collect the fluorescence signal. After conversion and comparison with a standard curve, the concentration of hydrogen peroxide in the cells is calculated, achieving accurate detection.
[0004] However, the aforementioned detection device still has some problems. In practical applications, in order to prevent external contaminants from interfering with cell samples, it is necessary to close the sealed door and check the sealing effect during the detection operation. However, in the existing technology, the drive motor cannot automatically shut off after the sealed door is closed, and it will continue to consume electrical energy. Secondly, the vacuum pump cannot automatically open to check the sealing effect, which makes it impossible to confirm the sealing of the detection box in time, affecting the accuracy of cell hydrogen peroxide detection. Therefore, a cell hydrogen peroxide sealed detection device is proposed to address the above problems. Summary of the Invention
[0005] To overcome the shortcomings of existing technologies and solve the problems mentioned in the background art, this utility model proposes a sealed detection device for hydrogen peroxide in cells.
[0006] The technical solution adopted by this utility model to solve its technical problem is as follows: The sealed hydrogen peroxide detection device for cells of this utility model includes a detection box. Two sealed doors are symmetrically installed on one side of the detection box. A groove is opened inside one side of one of the sealed doors. A long groove is opened inside the sealed door on one side of the groove. The radius of the groove is larger than the radius of the long groove. A short groove is opened inside one side of the sealed door. A movable rod is slidably installed between the long groove and the short groove. A circular block is installed at one end of the movable rod. The circular block is located inside the groove. A compression spring is fixed between the circular block and the long groove. The compression spring is sleeved on the outside of the movable rod. A connecting block is installed at the other end of the movable rod inside the short groove. An electrode plate is installed on one side of the connecting block. An electrode sheet is installed inside one side of the short groove. The electrode plate and the electrode sheet are on the same horizontal line. When the sealed door is closed and locked, the electrode plate and the electrode sheet contact each other, triggering a signal, driving the motor to automatically shut off and the vacuum pump to automatically turn on, thus simplifying the operation.
[0007] Preferably, two rotating shafts are symmetrically mounted inside one side of the testing box. A driving wheel and a driven wheel are respectively mounted on the outer side of the rotating shafts. The driving wheel and the driven wheel are connected by a belt. A rectangular groove is opened inside one side of the testing box, and a drive motor is installed inside the rectangular groove. One end of the rotating shaft is connected to the output end of the drive motor. Movable blocks are installed at the top and bottom of the belt, and sliders are installed on the bottom side of the movable blocks. A slide bar is installed on one side of the top of the testing box, and the sliders are slidably mounted at both ends of the slide bar. The sealing doors are respectively installed on the bottom side of the sliders, and handles are installed on one side of each sealing door. Through the drive of the drive motor and the cooperation of the driving wheel, driven wheel, and belt, the sealing doors can be automatically opened and closed, improving work efficiency.
[0008] Preferably, a telescopic rod is installed on the top inner wall of the detection box, and a detection camera is installed at the working end of the telescopic rod. A fluorescence sensor is installed inside the detection camera. The telescopic rod can adjust the position and height of the detection camera to adapt to different detection needs and ensure detection accuracy.
[0009] Preferably, a vacuum pump is installed on the other side of the testing chamber. The input pipe of the vacuum pump is connected to an air inlet pipe, one end of which extends into the top of the testing chamber. The output end of the vacuum pump is connected to an air outlet pipe. A pressure sensor is installed inside the testing chamber to monitor the pressure inside. A warning light is installed on one edge of the top of the testing chamber. The vacuum pump and pressure sensor work together to check the sealing effect of the testing chamber, ensure a stable testing environment, and improve the accuracy of the testing operation.
[0010] Preferably, a support base is installed inside the bottom side of the detection box, and a placement seat is installed on the top side of the support base. Two sliding grooves are symmetrically opened inside the placement seat. A support rod is fixed between the two sides of the sliding groove. A sliding block is slidably installed on the outside of the support rod inside the sliding groove. A fixing clamp is installed on the top side of each sliding block. A spring is fixed between the sliding block and the sliding groove. The spring is set on the outside of the support rod. Through the sliding block and the fixing clamp, the reaction dish containing cells and fluorescent needles is limited and controlled to ensure the stability of the reaction dish during the detection process.
[0011] Preferably, a control panel is installed on one side of the detection box. The control panel is electrically connected to the electrode plate and electrode sheet, the fluorescence sensor, the pressure sensor, the warning light, the drive motor, and the vacuum pump. It is used for signal transmission of the electrode plate and electrode sheet, the fluorescence sensor, and the pressure sensor, as well as operation control of the warning light, the drive motor, and the vacuum pump, thereby improving the overall automation level of the device.
[0012] The advantages of this utility model are:
[0013] 1. When the drive motor of this utility model rotates in the reverse direction, the sealing door will squeeze the circular block in another sealing door when the sealing door closes. This causes the movable rod to slide along the long groove and drive the connecting block to slide inside the short groove. This causes the connecting block to drive the electrode plate to gradually approach the electrode sheet. When the circular block is completely inside the groove, the electrode plate contacts the electrode sheet, generates an electrical signal and transmits it to the control panel. After receiving the signal, the control panel controls the drive motor to automatically shut off and simultaneously controls the vacuum pump to automatically turn on.
[0014] 2. In this invention, the reaction dish is placed on the base, and the reaction dish presses against the fixing plate, causing the sliding block to slide within the groove. Under the pressure of the reaction dish, the sliding block moves away from the center of the reaction dish, compressing the spring. Its elastic force pushes the sliding block towards the center of the reaction dish, causing the fixing plate to clamp the reaction dish, thus keeping the reaction dish stable. This ensures that the reaction between the cell sample and the fluorescent probe is not interfered with during the detection process, improving the accuracy of the detection results. Attached Figure Description
[0015] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0016] Figure 1 This is a schematic diagram of the intermediate axis side view of the present invention;
[0017] Figure 2 This is a schematic diagram of the internal structure of the detection device;
[0018] Figure 3 A schematic diagram of the sealing door opening and closing assembly of the testing device;
[0019] Figure 4 This is a schematic diagram of the trigger component structure;
[0020] Figure 5 This is a schematic diagram of the reaction vessel limiting and clamping assembly.
[0021] In the diagram: 1. Detection box; 2. Sealed door; 3. Groove; 4. Long groove; 5. Short groove; 6. Movable rod; 7. Circular block; 8. Compression spring; 9. Connecting block; 10. Electrode plate; 11. Electrode sheet; 12. Warning light; 13. Drive wheel; 14. Driven wheel; 15. Belt; 16. Drive motor; 17. Moving block; 18. Sliding bar; 19. Sliding block; 20. Handle; 21. Telescopic rod; 22. Detection camera; 23. Vacuum pump; 24. Inlet pipe; 25. Outlet pipe; 26. Pressure sensor; 27. Support base; 28. Placement base; 29. Slide groove; 30. Support rod; 31. Sliding block; 32. Fixing clamp; 33. Spring 1; 34. Control panel. Detailed Implementation
[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present utility model.
[0023] Please see Figure 1-4As shown, a sealed hydrogen peroxide detection device for cells includes a detection chamber 1. Two sealed doors 2 are symmetrically installed on one side of the detection chamber 1. A groove 3 is formed inside one side of one of the sealed doors 2. A long groove 4 is formed inside the sealed door 2 on one side of the groove 3, with the radius of the groove 3 being larger than the radius of the long groove 4. A short groove 5 is formed inside one side of the sealed door 2. A movable rod 6 is slidably installed between the long groove 4 and the short groove 5. A circular block 7 is installed at one end of the movable rod 6, located inside the groove 3. A compression spring 8 is fixed between the circular block 7 and the long groove 4, and the compression spring 8 is sleeved on the outside of the movable rod 6. A connecting block 9 is installed at the other end of the movable rod 6, located inside the short groove 5. An electrode plate 10 is installed on one side of the connecting block 9, and an electrode sheet 11 is installed inside one side of the short groove 5. The electrode plate 10 and the electrode sheet 11... 1. On the same horizontal line, a control panel 34 is installed on one side of the detection box 1. During operation, after the sealing door 2 is closed, the drive motor 16 cannot be automatically turned off, and will continue to consume power. Secondly, the vacuum pump 23 cannot be automatically turned on to check the sealing effect, which makes it impossible to confirm the sealing of the detection box 1 in time, affecting the accuracy of cell hydrogen peroxide detection. When the drive motor 16 rotates in the opposite direction, when the sealing door 2 is closed, one of the sealing doors 2 will squeeze the circular block 7 in the other sealing door 2, so that the movable rod 6 drives the connecting block 9 to slide inside the short groove 5 along the long groove 4, so that the connecting block 9 drives the electrode plate 10 to gradually approach the electrode sheet 11. When the circular block 7 is completely inside the groove 3, the electrode plate 10 contacts the electrode sheet 11, generates an electrical signal and transmits it to the control panel 34. After receiving the signal, the control panel 34 controls the drive motor 16 to automatically turn off, and at the same time controls the vacuum pump 23 to automatically turn on.
[0024] Please see Figure 1-4 As shown, two rotating shafts are symmetrically mounted inside one side of the detection box 1. A driving wheel 13 and a driven wheel 14 are respectively mounted on the outer side of the rotating shafts. The driving wheel 13 and the driven wheel 14 are connected by a belt 15. A rectangular groove is opened inside one side of the detection box 1. A drive motor 16 is installed inside the rectangular groove. One end of the rotating shaft is connected to the output end of the drive motor 16. Movable blocks 17 are installed at the top and bottom of the belt 15. Slider blocks 19 are installed on the bottom side of each movable block 17. A slide bar 18 is installed on one side of the top of the detection box 1. The sliders 19 are slidably mounted at both ends of the slide bar 18. Sealing doors 2 are installed on the bottom side of each slider 19. A handle 20 is installed on one side of each sealing door 2.
[0025] A vacuum pump 23 is installed on the other side of the detection chamber 1. The input pipe of the vacuum pump 23 is connected to an air inlet pipe 24. One end of the air inlet pipe 24 extends into the top of the detection chamber 1. The output end of the vacuum pump 23 is connected to an air outlet pipe 25. A pressure sensor 26 is installed inside the detection chamber 1 to monitor the pressure inside the chamber. A warning light 12 is installed on one edge of the top of the detection chamber 1. During operation, in an open environment, cell samples are easily affected by external factors. Oxygen, moisture, and various impurities in the air can react with the detection reagents, affecting the accuracy of the detection results. After the vacuum pump 23 is started, it draws air from the detection chamber 1 through the air inlet pipe 24. The gas enters the vacuum pump 23 and is then discharged through the outlet pipe 25. At this time, the pressure inside the detection chamber 1 gradually decreases. The pressure sensor 26 monitors the pressure change inside the detection chamber 1 in real time. If the detection chamber 1 is well sealed, the pressure detected by the pressure sensor 26 will continue to drop to the set negative pressure value and remain stable. If there is a leak in the detection chamber 1, the pressure detected by the pressure sensor 26 will drop at a slower rate and will not be able to reach the set negative pressure value, or the pressure will rise after reaching the negative pressure value. The pressure sensor 26 will transmit the detected pressure signal to the control panel 34. If the pressure change does not meet the standard of good sealing, the control panel 34 will control the warning light 12 to light up, prompting the operator that there is a problem with the sealing of the detection chamber 1. The specific model of the pressure sensor 26 is MS5803 series.
[0026] When the sealed door 2 needs to be opened, the drive motor 16 starts, and the output end of the drive motor 16 drives the rotating shaft to rotate. The drive wheel 13 installed on the rotating shaft rotates accordingly. The drive wheel 13 drives the driven wheel 14 to rotate through the belt 15. The rotation of the belt 15 drives the moving blocks 17 at its top and bottom to move. The slider 19 on the bottom side of the moving block 17 slides on the slide bar 18, thereby opening the sealed door 2.
[0027] Please see Figure 1 , 2 As shown in Figure 5, a telescopic rod 21 is installed on the top inner wall of the detection box 1, and a detection camera 22 is installed on the working end of the telescopic rod 21. A fluorescence sensor is installed inside the detection camera 22.
[0028] A support base 27 is installed inside the bottom side of the detection box 1, and a placement seat 28 is installed on the top side of the support base 27. Two symmetrical sliding grooves 29 are formed inside the placement seat 28. A support rod 30 is fixedly connected between the two sides of the sliding groove 29. A sliding block 31 is slidably installed on the outside of the support rod 30 inside the sliding groove 29. A fixing clamp 32 is installed on the top side of each sliding block 31. A spring 33 is fixedly connected between the sliding block 31 and the sliding groove 29, and the spring 33 is sleeved on the outside of the support rod 30. During operation, in cell physiological research, changes in the level of hydrogen peroxide in cells can reflect cell metabolism, signal transduction, etc. The state of the cells and fluorescent probes is important for understanding their energy metabolism, so a detection device is needed. First, the reaction dish containing the cells and fluorescent probes is placed on the placement seat 28. The reaction dish presses against the fixing plate 32, causing the sliding block 31 to slide within the groove 29. Under the pressure of the reaction dish, the sliding block 31 moves away from the center of the reaction dish. The spring 33 is compressed and generates elastic force, which pushes the sliding block 31 towards the center of the reaction dish, so that the fixing plate 32 clamps the reaction dish, keeping it stable and ensuring that the reaction of the cell sample and fluorescent probes is not disturbed during the detection process, thus improving the accuracy of the detection results.
[0029] When cellular hydrogen peroxide detection is required, the telescopic rod 21 can be extended or retracted via the control panel 34 according to the detection needs. As the telescopic rod 21 extends or retracts, the detection camera 22 installed at its active end moves up and down accordingly, adjusting to a suitable detection position height. The cell sample and fluorescent probe are mixed in the reaction dish within the placement seat 28. The hydrogen peroxide produced by the cells reacts with the fluorescent probe, generating a fluorescent signal. The fluorescence sensor inside the detection camera 22 receives the fluorescent signal and converts it into an electrical signal. The electrical signal is transmitted through the control panel 34. After data processing and analysis, combined with a pre-established standard curve, the concentration of cellular hydrogen peroxide is calculated. At the same time, the detection camera 22 can also image the sample in the reaction dish, recording the location and distribution of cellular hydrogen peroxide production. The specific model of the detection camera 22 is the Andor-Zyla series.
[0030] Working principle: When the drive motor 16 rotates in the reverse direction to close the sealing door 2, one of the sealing doors 2 will squeeze the circular block 7 in the other sealing door 2, causing the movable rod 6 to slide along the long groove 4 and drive the connecting block 9 to slide inside the short groove 5. This causes the connecting block 9 to drive the electrode plate 10 to gradually approach the electrode piece 11. When the circular block 7 is completely inside the groove 3, the electrode plate 10 contacts the electrode piece 11, generating an electrical signal and transmitting it to the control panel 34. After receiving the signal, the control panel 34 controls the drive motor 16 to automatically turn off and simultaneously controls the vacuum pump 23 to automatically turn on.
[0031] After the vacuum pump 23 starts, it draws air from the test chamber 1 through the air inlet pipe 24. The air in the test chamber 1 enters the vacuum pump 23 through the air inlet pipe 24 and is then discharged through the air outlet pipe 25. At this time, the pressure inside the test chamber 1 gradually decreases. The pressure sensor 26 monitors the pressure change inside the test chamber 1 in real time. If the test chamber 1 is well sealed, the pressure detected by the pressure sensor 26 will continue to drop to the set negative pressure value and remain stable. If there is a leak in the test chamber 1, the pressure drop rate detected by the pressure sensor 26 will slow down and will not reach the set negative pressure value, or the pressure will rise after reaching the negative pressure value. The pressure sensor 26 transmits the detected pressure signal to the control panel 34. If the pressure change does not meet the standard of good sealing, the control panel 34 controls the warning light 12 to light up, prompting the operator that there is a problem with the sealing of the test chamber 1. The specific model of the pressure sensor 26 is MS5803 series.
[0032] First, the reaction dish containing cells and fluorescent probes is placed on the placement seat 28. The reaction dish presses against the fixing clamp 32, causing the sliding block 31 to slide within the groove 29. Under the pressure of the reaction dish, the sliding block 31 moves away from the center of the reaction dish. The spring 33 is compressed and generates elastic force, which pushes the sliding block 31 towards the center of the reaction dish, so that the fixing clamp 32 clamps the reaction dish, making the reaction dish stable and ensuring that the reaction of cell samples and fluorescent probes is not interfered with during the detection process, thus improving the accuracy of the detection results.
[0033] When cellular hydrogen peroxide detection is required, the telescopic rod 21 can be extended or retracted via the control panel 34 according to the detection requirements. As the telescopic rod 21 extends or retracts, the detection camera 22 installed at its active end moves up and down accordingly to adjust to a suitable detection position height. The cell sample and fluorescent probe are mixed in the reaction dish within the placement seat 28. The hydrogen peroxide produced by the cells reacts with the fluorescent probe to generate a fluorescent signal. The fluorescence sensor inside the detection camera 22 receives the fluorescent signal and converts it into an electrical signal. The electrical signal is transmitted through the control panel 34. After data processing and analysis, combined with a pre-established standard curve, the concentration of cellular hydrogen peroxide is calculated. At the same time, the detection camera 22 can also image the sample in the reaction dish and record the location and distribution of cellular hydrogen peroxide production. The specific model of the detection camera 22 is the Andor-Zyla series.
[0034] When the sealed door 2 needs to be opened, the drive motor 16 starts, and the output end of the drive motor 16 drives the rotating shaft to rotate. The drive wheel 13 installed on the rotating shaft rotates accordingly. The drive wheel 13 drives the driven wheel 14 to rotate through the belt 15. The rotation of the belt 15 drives the moving blocks 17 at its top and bottom to move. The slider 19 on the bottom side of the moving block 17 slides on the slide bar 18, thereby opening the sealed door 2.
Claims
1. A sealed detection device for hydrogen peroxide in cells, characterized in that: The test box (1) includes two symmetrically installed sealing doors (2) on one side. One of the sealing doors (2) has a groove (3) inside its side. A long groove (4) is opened inside the sealing door (2) on one side of the groove (3). The radius of the groove (3) is larger than the radius of the long groove (4). A short groove (5) is opened inside the sealing door (2). A movable rod (6) is slidably installed between the long groove (4) and the short groove (5). A circular rod is installed at one end of the movable rod (6). Block (7), the circular block (7) is located inside the groove (3), and a compression spring (8) is fixed between the circular block (7) and the long groove (4). The compression spring (8) is sleeved on the outside of the movable rod (6). The other end of the movable rod (6) is located inside the short groove (5) and a connecting block (9) is installed. An electrode plate (10) is installed on one side of the connecting block (9), and an electrode sheet (11) is installed inside one side of the short groove (5). The electrode plate (10) and the electrode sheet (11) are on the same horizontal line.
2. The sealed detection device for hydrogen peroxide in cells according to claim 1, characterized in that: Two rotating shafts are symmetrically installed inside one side of the detection box (1). A driving wheel (13) and a driven wheel (14) are respectively installed on the outer side of the rotating shaft. The driving wheel (13) and the driven wheel (14) are connected by a belt (15). A rectangular groove is opened inside one side of the detection box (1). A drive motor (16) is installed inside the rectangular groove. One end of the rotating shaft is connected to the output end of the drive motor (16). A moving block (17) is installed at the top and bottom of the belt (15). A slider (19) is installed on the bottom side of the moving block (17). A slide bar (18) is installed on one side of the top of the detection box (1). The slider (19) is slidably installed at both ends of the slide bar (18). The sealing door (2) is installed on the bottom side of the slider (19). A handle (20) is installed on one side of the sealing door (2).
3. The sealed detection device for hydrogen peroxide in cells according to claim 2, characterized in that: A telescopic rod (21) is installed on the inner wall of the top side of the detection box (1). A detection camera (22) is installed at the working end of the telescopic rod (21). A fluorescence sensor is installed inside the detection camera (22).
4. The sealed detection device for hydrogen peroxide in cells according to claim 3, characterized in that: A vacuum pump (23) is installed on the other side of the test box (1). The input pipe of the vacuum pump (23) is connected to an air inlet pipe (24). One end of the air inlet pipe (24) extends to the top of the test box (1). The output end of the vacuum pump (23) is connected to an air outlet pipe (25). A pressure sensor (26) is installed on the inside of the test box (1). The pressure sensor (26) is used to monitor the pressure inside the test box (1). A warning light (12) is installed on one edge of the top of the test box (1).
5. A sealed cellular hydrogen peroxide detection device according to claim 4, characterized in that: The bottom side of the test box (1) is equipped with a support base (27), and the top side of the support base (27) is equipped with a placement base (28). The placement base (28) has two symmetrical sliding grooves (29) inside. A support rod (30) is fixed between the two sides of the sliding groove (29). A sliding block (31) is slidably installed on the outside of the support rod (30) inside the sliding groove (29). A fixing clamp (32) is installed on the top side of each sliding block (31). A spring (33) is fixed between the sliding block (31) and the sliding groove (29). The spring (33) is sleeved on the outside of the support rod (30).
6. The sealed detection device for hydrogen peroxide in cells according to claim 5, characterized in that: A control panel (34) is installed on one side of the detection box (1). The control panel (34) is electrically connected to the electrode plate (10) and electrode sheet (11), fluorescence sensor, pressure sensor (26), warning light (12), drive motor (16) and vacuum pump (23). It is used for signal transmission of the electrode plate (10) and electrode sheet (11), fluorescence sensor, pressure sensor (26) and operation control of warning light (12), drive motor (16) and vacuum pump (23).