Oscillation auxiliary type water bath protein enzyme digestion device

Abstract

The utility model discloses an oscillation auxiliary type water bath protein enzyme digestion device which comprises an oscillation auxiliary type water bath box, an enzyme storage box, a water adding pipe and a feeding pipe are installed on the oscillation auxiliary type water bath box, a stirring mechanism and an adjusting mechanism are installed on the oscillation auxiliary type water bath box, and the adjusting mechanism is installed on the feeding pipe. Through the arrangement of a servo motor, a rotating shaft, a stirring rod, a driving ring, a limiting sliding block, a waterproof electric telescopic rod, a connecting cylinder and other structures, when the servo motor drives the stirring rod to rotate to stir raw materials and water put into the oscillation auxiliary water bath box, the waterproof electric telescopic rod is started to drive the driving ring to move downwards; therefore, the driving ring can be conveniently utilized to drive the stirring rod to perform arc-shaped rotation on the retention block, and then the stirring rod can be conveniently utilized to fully stir and mix different layers of raw materials and proteolytic enzyme in the oscillation-assisted water bath box.

Description

TECHNICAL FIELD

[0001] The utility model relates to protein enzymolysis technical field, concretely is a kind of oscillation auxiliary formula water bath proteinase device. BACKGROUND

[0002] Enzymatic digestion is an important technology in biology, which involves the use of specific restriction enzymes to cut DNA molecules. Enzymatic digestion is the process of cutting DNA molecules, usually using restriction enzymes, which can recognize and cut specific nucleotide sequences in DNA molecules. Enzymatic digestion is an important biological technology with wide application value. When performing enzymatic digestion experiments, enzymes need to be carefully selected, reaction conditions need to be optimized, DNA template quality needs to be ensured, and possible problems and solutions need to be noted. At the same time, in order to fully and uniformly water bath during the enzymatic digestion process, an oscillation auxiliary water bath device is used. The oscillation auxiliary water bath device generates heat and water flow movement through electromagnetic oscillation, and realizes uniform mixing and reaction acceleration of the sample at a constant temperature through a heating control system. This device has wide application in chemical experiments, biological experiments and other fields.

[0003] In the prior art, when using the oscillation auxiliary water bath proteinase device, the existing stirring device cannot stir at different levels, resulting in insufficient stirring. At the same time, the existing raw material adding device can only add from the fixed position above, and in the traditional water bath enzymatic digestion, the raw materials cannot be fully mixed with water, so that the protein in the raw materials cannot be fully dissolved and separated when adding proteinase later, which can cause insufficient enzymatic digestion.

[0004] Therefore, an oscillation auxiliary water bath proteinase device is proposed. UTILITY MODEL CONTENT

[0005] The utility model aims to provide an oscillation auxiliary water bath proteinase device to solve the problems raised in the background art.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: an oscillation auxiliary water bath proteinase device, comprising an oscillation auxiliary water bath box, an enzyme storage box, a water adding pipe and a material adding pipe are installed on the oscillation auxiliary water bath box, a stirring mechanism and an adjusting mechanism are installed on the oscillation auxiliary water bath box, the adjusting mechanism is installed on the material adding pipe, the stirring mechanism comprises a rotating shaft, the rotating shaft is rotatably installed on the oscillation auxiliary water bath box, a plurality of retaining blocks are annularly and equidistantly installed on the rotating shaft, a plurality of stirring rods are rotatably installed on the retaining blocks, a driving ring is slidably installed on the rotating shaft, a plurality of adjusting rods are rotatably installed on the driving ring, a plurality of positioning blocks are rotatably installed on the other ends of the adjusting rods, and the positioning blocks are respectively installed on the stirring rods.

[0007] Preferably, the oscillation auxiliary water bath box is provided with a mounting frame, a servo motor is mounted on the mounting frame, a driving shaft is mounted on the servo motor, and the driving shaft is mounted on the top end of the rotating shaft.

[0008] Preferably, a plurality of limiting grooves are formed in the rotating shaft, a limiting slider is slidably mounted on the inner wall of each limiting groove, and the plurality of limiting sliders are mounted on the inner wall of the driving ring.

[0009] Preferably, a waterproof electric telescopic rod is mounted on the inner wall of the bottom side of the oscillation auxiliary water bath box, a connecting barrel is rotatably mounted on the waterproof electric telescopic rod, a plurality of connecting rods are mounted on the connecting barrel, and the plurality of connecting rods are mounted on one of the driving rings.

[0010] Preferably, the adjusting mechanism comprises a T-shaped block, the T-shaped block is slidably mounted on the inner wall of the oscillation auxiliary water bath box, an active ring is mounted on the T-shaped block, a retaining sleeve is mounted on the outer surface of the active ring, and the feeding pipe is mounted on the retaining sleeve.

[0011] Preferably, a transmission assembly is sleeved on the driving shaft, an adapter shaft is sleeved on the other end of the transmission assembly, a rotating shaft is mounted on the bottom side of the adapter shaft, and the bottom end of the rotating shaft is rotatably mounted on the inner wall of the bottom side of the oscillation auxiliary water bath box.

[0012] Preferably, an elliptical groove is formed in the rotating shaft, an active shaft is slidably mounted on the inner wall of the elliptical groove, and the other end of the active shaft is mounted on the inner wall of the active ring.

[0013] Compared with the prior art, the utility model has the advantages that:

[0014] (1) The utility model discloses a servo motor, a rotating shaft, a stirring rod, a driving ring, a limiting slider, a waterproof electric telescopic rod and a connecting barrel are arranged, when the stirring rod is driven to rotate by the servo motor to stir the raw materials and water put into the oscillation auxiliary water bath box, the waterproof electric telescopic rod is started to drive the driving ring to move downwards, so that the stirring rod can be conveniently driven to make arc rotation on the retaining block by the driving ring, and the raw materials and protein decomposition enzyme in different layers in the oscillation auxiliary water bath box can be conveniently and fully stirred and mixed by the stirring rod.

[0015] (2) The utility model discloses a rotating shaft, an elliptical groove, an active ring, a retaining sleeve and a transmission assembly are arranged, when in use, the rotating shaft is driven to rotate by the driving shaft, the feeding pipe on the retaining sleeve makes reciprocating motion in the vertical direction in the oscillation auxiliary water bath box by the rotating shaft, so that the raw materials can be conveniently put into the oscillation auxiliary water bath box from different positions for mixing, and the raw materials and protein decomposition enzyme can be fully mixed. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 It is the main structure schematic view of the utility model;

[0017] Figure 2 It is the internal structure schematic view of the oscillation auxiliary type water bath box of the utility model;

[0018] Figure 3 It is the connecting structure schematic view of the waterproof electric telescopic rod, connecting cylinder and connecting rod of the utility model;

[0019] Figure 4 It is the connecting structure schematic view of the feeding pipe, movable ring and rotating shaft of the utility model;

[0020] In the drawing: 1, oscillation auxiliary type water bath box;2, enzyme storage box;3, water feeding pipe;4, feeding pipe;5, rotating shaft;51, retaining block;52, stirring rod;53, driving ring;54, adjusting rod;55, positioning block;56, limiting groove;57, limiting sliding block;58, mounting frame;59, servo motor;510, driving shaft;511, waterproof electric telescopic rod;512, connecting cylinder;513, connecting rod;6, T-shaped block;61, movable ring;62, retaining sleeve;63, rotating shaft;64, oval groove;65, movable shaft;66, transmission assembly;67, adapter shaft. DETAILED DESCRIPTION

[0021] The technical scheme in the embodiments of the utility model will be described clearly and completely below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.

[0022] Embodiment 1: please refer to Figures 1-4The utility model provides a technical scheme: an oscillation auxiliary type water bath protein enzyme cutting device, including oscillation auxiliary type water bath box 1, install enzyme storage box 2, water adding pipe 3 and charging pipe 4 on oscillation auxiliary type water bath box 1, install stirring mechanism and adjusting mechanism on oscillation auxiliary type water bath box 1, adjusting mechanism installs on charging pipe 4, and stirring mechanism includes rotating shaft 5, and rotating shaft 5 is rotatably installed on oscillation auxiliary type water bath box 1, a plurality of retaining blocks 51 are installed on rotating shaft 5 in annular equidistance, a plurality of stirring rods 52 are rotatably installed on a plurality of retaining blocks 51, and driving ring 53 is slidably installed on rotating shaft 5, a plurality of limit grooves 56 are formed on rotating shaft 5, and limit sliding block 57 is slidably installed on the inner wall of a plurality of limit grooves 56, a plurality of limit sliding blocks 57 are installed on the inner wall of driving ring 53, a plurality of adjusting rods 54 are rotatably installed on driving ring 53, and positioning block 55 is rotatably installed on the other end of a plurality of adjusting rods 54, and a plurality of positioning blocks 55 are installed on a plurality of stirring rods 52 respectively, utilize rotating shaft 5 to drive retaining block 51 to rotate, and retaining block 51 drives stirring rod 52 to rotate, can conveniently mix raw materials and protein decomposition enzyme in oscillation auxiliary type water bath box 1, simultaneously utilize driving ring 53 to drive adjusting rod 54 to rotate, and adjusting rod 54 drives stirring rod 52 to do arc rotation, to effectively change the stirring range of stirring rod 52, make raw materials and protein decomposition enzyme mix more thoroughly.

[0023] Further, the oscillation auxiliary type water bath box 1 is installed on the mounting bracket 58, the servo motor 59 is installed on the mounting bracket 58, the drive shaft 510 is installed on the servo motor 59, the drive shaft 510 is installed on the top end of the rotating shaft 5, the servo motor 59 drives the drive shaft 510 to rotate, the drive shaft 510 drives the rotating shaft 5 to rotate, thereby providing power for the rotation of the stirring rod 52.

[0024] Further, the waterproof electric telescopic rod 511 is installed on the inner wall of the bottom side of the oscillation auxiliary type water bath box 1, the connecting cylinder 512 is rotatably installed on the waterproof electric telescopic rod 511, a plurality of connecting rods 513 are installed on the connecting cylinder 512, a plurality of connecting rods 513 are installed on one of the driving rings 53, the connecting cylinder 512 is moved by the waterproof electric telescopic rod 511, the connecting rod 513 is moved by the connecting cylinder 512, the driving ring 53 is moved up and down by the connecting rod 513, thereby providing power for the arc rotation of the stirring rod 52.

[0025] In example 2, on the basis of example 1, in order to enable the raw materials to be fully mixed in the oscillation-assisted water bath box 1, an adjusting mechanism is arranged on the oscillation-assisted water bath box 1, which specifically comprises a T-shaped block 6 that is slidingly installed on the inner wall of the oscillation-assisted water bath box 1, an activity ring 61 that is installed on the T-shaped block 6, a retaining sleeve 62 that is installed on the outer surface of the activity ring 61, the charging pipe 4 that is installed on the retaining sleeve 62, a transmission assembly 66 that is sleeved on the driving shaft 510, an adapter shaft 67 that is sleeved on the other end of the transmission assembly 66, a rotating shaft 63 that is installed on the bottom side of the adapter shaft 67, the rotating shaft 63 that is rotationally installed on the bottom side inner wall of the oscillation-assisted water bath box 1, an oval groove 64 that is opened on the rotating shaft 63, an activity shaft 65 that is slidingly installed on the inner wall of the oval groove 64, and the other end of the activity shaft 65 that is installed on the inner wall of the activity ring 61. The raw materials are injected into the oscillation-assisted water bath box 1 through the charging pipe 4, and then the switch on the servo motor 59 is started to drive the driving shaft 510 to rotate, the driving shaft 510 drives the transmission assembly 66 to rotate, the transmission assembly 66 drives the adapter shaft 67 to rotate, the adapter shaft 67 drives the rotating shaft 63 to rotate, the rotating shaft 63 drives the oval groove 64 to rotate, the oval groove 64 drives the activity shaft 65 to do reciprocating motion in the vertical direction, the activity shaft 65 drives the activity ring 61 under the restriction of the T-shaped block 6 to move, and the activity ring 61 drives the charging pipe 4 fixed on the retaining sleeve 62 to move, so that the raw materials can be conveniently put into the water in the oscillation-assisted water bath box 1 from different positions. The remaining features are the same as those of example 1.

[0026] The working principle is that: when the protein is enzymatically cut, the raw materials and water are water-bathed by the oscillation-assisted water bath box, water is injected into the oscillation-assisted water bath box 1 through the water injection pipe 3, the raw materials are injected into the oscillation-assisted water bath box 1 through the feeding pipe 4, then the switch on the servo motor 59 is started, the servo motor 59 drives the driving shaft 510 to rotate, the driving shaft 510 drives the transmission assembly 66 to rotate, the transmission assembly 66 drives the adapter shaft 67 to rotate, the adapter shaft 67 drives the rotating shaft 63 to rotate, the rotating shaft 63 drives the elliptical groove 64 to rotate, the elliptical groove 64 drives the movable shaft 65 to do reciprocating motion in the vertical direction, the movable shaft 65 drives the movable ring 61 under the restriction of the T-shaped block 6 to move, the movable ring 61 drives the feeding pipe 4 fixed on the retaining sleeve 62 to move, so that the raw materials can be conveniently put into the water in the oscillation-assisted water bath box 1 from different positions, at the same time, the protein decomposition enzyme is put into the oscillation-assisted water bath box 1 through the rotating shaft 5, and the rotating shaft 5 is driven by the driving shaft 510 to rotate, the rotating shaft 5 drives the stirring rod 52 on the retaining block 51 to rotate, when it is needed to change the stirring area, only the switch on the waterproof electric telescopic rod 511 is started, the waterproof electric telescopic rod 511 drives the connecting barrel 512 to move downward, the waterproof electric telescopic rod 511 drives the connecting barrel 512 to move downward, the connecting barrel 512 drives the connecting rod 513 to move, the connecting rod 513 drives the driving ring 53 to move downward, the driving ring 53 drives the adjusting rod 54 to rotate, the adjusting rod 54 drives the stirring rod 52 to do arc-shaped rotation, so that the stirring range of the stirring rod 52 can be conveniently changed, the protein decomposition enzyme and the raw materials can be fully mixed, and then the protein enzymatic cutting is facilitated to be more thorough.

[0027] Although the embodiments of the present application have been shown and described, it should be understood by those skilled in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present application, and the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. An oscillation-assisted water bath protein enzyme cutting device, comprising an oscillation-assisted water bath box (1), an enzyme storage box (2), a water adding pipe (3) and a material adding pipe (4) are installed on the oscillation-assisted water bath box (1), characterized in that: The oscillation auxiliary water bath box (1) is provided with a stirring mechanism and an adjusting mechanism, the adjusting mechanism is installed on the feeding pipe (4), the stirring mechanism comprises a rotating shaft (5), the rotating shaft (5) is rotatably installed on the oscillation auxiliary water bath box (1), a plurality of retaining blocks (51) are annularly and equidistantly installed on the rotating shaft (5), a plurality of stirring rods (52) are rotatably installed on the retaining blocks (51), a driving ring (53) is slidably installed on the rotating shaft (5), a plurality of adjusting rods (54) are rotatably installed on the driving ring (53), a plurality of positioning blocks (55) are rotatably installed on the other ends of the adjusting rods (54), and the positioning blocks (55) are installed on the stirring rods (52) respectively.

2. The oscillation-assisted water bath protein digestion device according to claim 1, wherein: The oscillation auxiliary water bath box (1) is provided with a mounting rack (58), a servo motor (59) is installed on the mounting rack (58), a driving shaft (510) is installed on the servo motor (59), and the driving shaft (510) is installed on the top end of the rotating shaft (5).

3. The oscillation-assisted water bath protein digestion device according to claim 1, wherein: A plurality of limiting grooves (56) are formed in the rotating shaft (5), limiting sliding blocks (57) are slidably installed on the inner walls of the limiting grooves (56), and the limiting sliding blocks (57) are installed on the inner walls of the driving rings (53).

4. The oscillation-assisted water bath protein digestion device of claim 1, wherein: A waterproof electric telescopic rod (511) is installed on the inner wall of the bottom side of the oscillation auxiliary water bath box (1), a connecting barrel (512) is rotatably installed on the waterproof electric telescopic rod (511), a plurality of connecting rods (513) are installed on the connecting barrel (512), and the connecting rods (513) are installed on one of the driving rings (53).

5. The oscillation-assisted water bath protein digestion device of claim 2, wherein: The adjusting mechanism comprises a T-shaped block (6), the T-shaped block (6) is slidably installed on the inner wall of the oscillation auxiliary water bath box (1), a movable ring (61) is installed on the T-shaped block (6), a retaining sleeve (62) is installed on the outer surface of the movable ring (61), and the feeding pipe (4) is installed on the retaining sleeve (62).

6. The oscillation-assisted water bath protein digestion device of claim 5, wherein: A transmission assembly (66) is sleeved on the driving shaft (510), an adapter shaft (67) is sleeved on the other end of the transmission assembly (66), a rotating shaft (63) is installed on the bottom side of the adapter shaft (67), and the bottom end of the rotating shaft (63) is rotatably installed on the inner wall of the bottom side of the oscillation auxiliary water bath box (1).

7. The oscillation-assisted water bath protein digestion device of claim 6, wherein: An oval groove (64) is formed in the rotating shaft (63), a movable shaft (65) is slidably installed on the inner wall of the oval groove (64), and the other end of the movable shaft (65) is installed on the inner wall of the movable ring (61).

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