Magnetic bead purification device for biological experiment

Abstract

The utility model discloses a magnetic bead purification device for biological experiments. The magnetic bead purification device comprises a base, a first driving motor is fixedly connected with a rotating rod through a coupler, an adjusting plate is fixedly installed at the upper end of the rotating rod, magnetic beads are arranged in a centrifugal tube, and a third magnet block is arranged on the side portion of a lifting block. Through the design of structures such as a first driving motor, an adjusting plate and a mounting seat, a centrifugal tube filled with target molecules or cells is mounted on the mounting seat in advance, and the first driving motor, a rotating rod and the adjusting plate are matched, so that the mounting seat can be driven to rotate, and the centrifugal tube with enriched magnetic beads and the centrifugal tube with non-enriched magnetic beads move; according to the magnetic bead enrichment device, the centrifugal tube can be replaced after the magnetic beads are enriched, the sliding block can be pushed to move through the air cylinder, so that the magnetic beads are enriched after the centrifugal tube is close to the third magnet block, the function of replacing the centrifugal tube can be provided, the shutdown time when the centrifugal tube is replaced can be shortened, and the magnetic bead enrichment efficiency can be improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of magnetic bead purification technology for biological experiments, and particularly to a magnetic bead purification device for biological experiments. BACKGROUND

[0002] The magnetic stand is used for separating samples by magnetic beads, and the magnetic beads can be used for separating cells, proteins, nucleic acids or other biological samples. First, the magnetic beads are mixed with the sample in a 1.5 milliliter centrifuge tube at a proper ratio. Within a few minutes, the magnetic beads can be combined with the target molecules or cells in the sample. Then, the 1.5 milliliter centrifuge tube is placed in a magnetic field, and under the action of the magnetic force, the target molecules or cells combined with the magnetic beads can be adsorbed on the tube wall. After discarding the supernatant, the purified target molecules or cells can be obtained.

[0003] A magnetic bead purification device for biological experiments is provided in the prior art (publication number CN218146600U). The utility model includes a base plate, a vertical plate fixedly connected to the top of the base plate, a recess formed in one side of the vertical plate, a limiting frame fixedly connected to the outer wall of one side of the vertical plate, and a sleeve block sleeved with the outer wall of the vertical plate. When the sleeve block slides along the outer wall of the vertical plate, the recess slides along the outer wall of the convex plate, and the whole is more stable. When the sleeve block moves up and down, the magnet block and the silica gel plate simultaneously slide along the outer wall of the centrifuge tube. The magnet block and the magnetic beads are magnetically attracted to each other. The position of the magnetic beads can be adjusted synchronously by adjusting the position of the magnet block. After the position is adjusted, the suction cup and the recess on the vertical plate are attached to each other and are fixed by mutual attraction. The magnet block reciprocally moves in the direction in which the centrifuge tube is vertically placed, which can quickly and uniformly adsorb the molecules or cells combined with the magnetic beads on the tube wall of the centrifuge tube, thereby ensuring the purity of the obtained molecules or cells.

[0004] The above-mentioned magnetic bead purification device for biological experiments still has some problems in actual operation, such as the need to disassemble the centrifuge tube after the magnetic beads are enriched before replacing the centrifuge tube, which results in a long downtime during replacement and reduces the enrichment efficiency of the magnetic beads. Therefore, a magnetic bead purification device for biological experiments is needed. UTILITY MODEL CONTENTS

[0005] The utility model aims to provide a magnetic bead purification device for biological experiments, which can improve the function of replacing the centrifuge tube, reduce the downtime during replacement of the centrifuge tube, and improve the enrichment efficiency of the magnetic beads to solve the problems in the above background.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical scheme:

[0007] The utility model provides a kind of magnetic bead purification device for biological experiment, including base, the top of the base is fixedly installed with support, the bottom of the support is fixedly installed with first drive motor, the first drive motor is fixedly connected with rotating rod by shaft coupling, the upper end of the rotating rod is fixedly installed with adjusting plate, multiple sets of reset mechanism are arranged in the adjusting plate, multiple sets of the reset mechanism are all provided with mounting seat, the top of the mounting seat is provided with fixed mechanism, the top of the mounting seat is fixedly installed with centrifugal tube by fixed mechanism, the centrifugal tube is provided with magnetic bead;

[0008] The top of the base is provided with a reciprocating adjustment mechanism, and a lifting block is arranged on the reciprocating adjustment mechanism.

[0009] Preferably, the top of the mounting seat is provided with a mounting groove, and the lower end of the centrifugal tube is arranged in the mounting groove.

[0010] Preferably, the fixed mechanism includes a receiving tube, a first magnet block, an adjusting seat, a pressing seat, a positioning block, and a second magnet block.

[0011] Preferably, the positioning block is fixedly installed at the bottom of the pressing seat, and the positioning block is inserted into the centrifugal tube.

[0012] Preferably, the reset mechanism includes a positioning rod, a reset spring, a sliding block, and a push block.

[0013] Preferably, the mounting seat is fixedly installed at the top of the sliding block, and the push block is fixedly installed at the bottom of the sliding block.

[0014] Preferably, the side of the support is fixedly installed with an air cylinder for pushing the push block.

[0015] Preferably, the reciprocating adjustment mechanism includes a fixed frame, a second drive motor, and a reciprocating screw rod.

[0016] Compared with the prior art, the utility model has the beneficial effects that:

[0017] Through the design of first drive motor, adjusting plate and mounting seat and other structures, through the advance that installs the centrifugal tube that is equipped with target molecule or cell in mounting seat, through first drive motor, rotating lever and adjusting plate cooperation, can drive mounting seat rotation, make centrifugal tube after magnetic bead enrichment and centrifugal tube that magnetic bead is not enriched move, realize the replacement of centrifugal tube after magnetic bead enrichment, through the cylinder can push the slider to move, make centrifugal tube close third magnet block after magnetic bead enrichment, the utility model discloses can provide the function of centrifugal tube replacement, can reduce the time of stop machine when replacing centrifugal tube, thereby can improve the efficiency of magnetic bead enrichment. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 It is the structural schematic diagram of the utility model;

[0019] Figure 2 It is the structural schematic diagram of adjusting plate, support and mounting seat and other structures of the utility model;

[0020] Figure 3 It is the structural schematic diagram of adjusting plate section of the utility model;

[0021] Figure 4 It is the structural schematic diagram of the utility model Figure 3 A zone amplification structure schematic diagram in the utility model.

[0022] In the drawing: 1, base;2, support;3, first drive motor;4, rotating lever;5, adjusting plate;6, sliding slot;7, positioning rod;8, return spring;9, slider;10, push block;11, cylinder;12, mounting seat;13, centrifugal tube;14, magnetic bead;15, storage tube;16, first magnet block;17, adjusting seat;18, pressing seat;19, positioning block;20, second magnet block;21, avoiding groove;22, fixed frame;23, second drive motor;24, reciprocating screw;25, lifting block;26, third magnet block. DETAILED DESCRIPTION

[0023] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.

[0024] Please refer to Figures 1-4 The utility model provides a technical scheme:

[0025] The application discloses a magnetic bead purification device for biological experiments, which comprises a base 1, a support 2 fixedly arranged on the top of the base 1, a first driving motor 3 fixedly arranged on the bottom of the support 2, a rotating rod 4 fixedly connected with the first driving motor 3 through a shaft coupling, an adjusting plate 5 fixedly arranged on the upper end of the rotating rod 4, a plurality of reset mechanisms arranged in the adjusting plate 5, a mounting seat 12 arranged on each of the reset mechanisms, a fixing mechanism arranged on the top of the mounting seat 12, a centrifugal tube 13 fixedly arranged on the top of the mounting seat 12 through the fixing mechanism, and magnetic beads 14 arranged in the centrifugal tube 13.

[0026] A reciprocating adjusting mechanism is arranged on the top of the base 1, and a lifting block 25 is arranged on the reciprocating adjusting mechanism.

[0027] In an optional embodiment, the top of the mounting seat 12 is provided with a mounting groove, the lower end of the centrifugal tube 13 is arranged in the mounting groove, and an avoiding groove 21 for inserting the lifting block 25 is arranged on the mounting seat 12 and communicates with the mounting groove.

[0028] In an optional embodiment, the fixing mechanism comprises a receiving tube 15, a first magnet block 16, an adjusting seat 17, a pressing seat 18, a positioning block 19 and a second magnet block 20, the receiving tube 15 is fixedly arranged on the mounting seat 12, the first magnet block 16 is fixedly arranged on the inner bottom of the receiving tube 15, the pressing seat 18 is movably arranged on the top of the adjusting seat 17, the adjusting seat 17 is movably inserted into the adjusting plate 5, the second magnet block 20 is fixedly arranged on the bottom of the adjusting seat 17, and the first magnet block 16 and the second magnet block 20 are mutually adsorbed.

[0029] In an optional embodiment, the positioning block 19 is fixedly arranged on the bottom of the pressing seat 18 and is inserted into the centrifugal tube 13.

[0030] It should be noted that the positioning block 19 is inserted into the centrifugal tube 13 through mutual adsorption of the first magnet block 16 and the second magnet block 20, so that the centrifugal tube 13 can be fixed on the mounting seat 12.

[0031] In an optional embodiment, the reset mechanism comprises a positioning rod 7, a reset spring 8, a sliding block 9 and a pushing block 10, a plurality of sliding grooves 6 are arranged in the adjusting plate 5, the positioning rod 7 is fixedly arranged on the inner wall of the sliding groove 6, the reset spring 8 is sleeved on the positioning rod 7, the sliding block 9 is slidingly arranged on the positioning rod 7, one end of the reset spring 8 abuts against the inner wall of the sliding groove 6, and the other end of the reset spring 8 abuts against the side of the sliding block 9.

[0032] In an optional embodiment, the mounting seat 12 is fixedly arranged on the top of the sliding block 9, and the pushing block 10 is fixedly arranged on the bottom of the sliding block 9.

[0033] In an optional embodiment: the side of the support 2 is fixedly installed with a cylinder 11 for pushing the push block 10.

[0034] It should be noted that through the cooperation of the positioning rod 7, the return spring 8, the sliding block 9 and the push block 10, after the telescopic rod of the cylinder 11 retracts, the return spring 8 can push the sliding block 9, so that the sliding block 9 can drive the mounting seat 12 to move, so that the centrifugal tube 13 can return to the original position.

[0035] In an optional embodiment: the reciprocating adjusting mechanism comprises a fixed frame 22, a second driving motor 23 and a reciprocating screw rod 24, the fixed frame 22 is fixedly installed on the top of the base 1, the second driving motor 23 is fixedly installed on the top of the fixed frame 22, the reciprocating screw rod 24 is fixedly connected on the output shaft of the second driving motor 23 through a shaft coupling, and the lifting block 25 is screwed on the reciprocating screw rod 24.

[0036] It should be noted that through the cooperation of the second driving motor 23, the lifting block 25 and the reciprocating screw rod 24, the third magnet block 26 can be driven to move, so that the third magnet block 26 can reciprocate in the direction of vertical placement of the magnetic beads 14 in the centrifugal tube 13, which can improve the enrichment efficiency and effectively shorten the magnetic bead enrichment time.

[0037] In specific use, first, the lower end of the centrifugal tube 13 is placed in the mounting groove on the top of the mounting seat 12, the positioning block 19 at the bottom of the pressing seat 18 is moved above the centrifugal tube 13 by rotating the pressing seat 18, and then the positioning block 19 is inserted into the centrifugal tube 13, at this time the first magnet block 16 and the second magnet block 20 are mutually adsorbed, so that the centrifugal tube 13 can be fixed through the cooperation of the pressing seat 18 and the positioning block 19;

[0038] By starting the cylinder 11, the telescopic rod of the cylinder 11 pushes the push block 10, so that the push block 10 can drive the sliding block 9 to move when moving, so that the sliding block 9 can drive the centrifugal tube 13 to move close to the third magnet block 26, so that the third magnet block 26 can contact the surface of the centrifugal tube 13, so that the magnetic beads 14 can be adsorbed on the centrifugal tube 13, by starting the second driving motor 23, the output shaft of the second driving motor 23 rotates to drive the reciprocating screw rod 24 to rotate, so that the reciprocating screw rod 24 can drive the third magnet block 26 to move when rotating, so that the third magnet block 26 can drive the magnetic beads 14 to move when moving, so that the magnetic beads 14 can reciprocate in the direction of vertical placement in the centrifugal tube 13, which can improve the enrichment efficiency and effectively shorten the magnetic bead enrichment time.

[0039] After the magnetic beads are enriched, the air cylinder 11 works, the telescopic rod of the air cylinder 11 is retracted, the reset spring 8 can push the sliding block 9, so that the sliding block 9 can drive the mounting seat 12 to move, so that the centrifugal tube 13 can return to the original position, the first driving motor 3 is started, the output shaft of the first driving motor 3 rotates to drive the rotating rod 4 to rotate, so that the rotating rod 4 drives the adjusting plate 5 to rotate when rotating, the mounting seat 12 can be driven to rotate through the rotating of the adjusting plate 5, so that the centrifugal tube 13 after the magnetic beads are enriched and the centrifugal tube 13 before the magnetic beads are enriched are moved, the replacement of the centrifugal tube 13 after the magnetic beads are enriched is realized, the push block 10 can be pushed to move through the air cylinder 11, so that the centrifugal tube 13 is close to the third magnet block 26 to carry out the enrichment of the magnetic beads.

[0040] Although the embodiments of the present application have been shown and described, it is to be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present application, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A magnetic bead purification device for biological experiments, comprising a base (1), characterized in that The top of the base (1) is fixedly provided with a support (2), the bottom of the support (2) is fixedly provided with a first driving motor (3), the first driving motor (3) is fixedly connected with a rotating rod (4) through a shaft coupling, the upper end of the rotating rod (4) is fixedly provided with an adjusting plate (5), a plurality of reset mechanisms are arranged in the adjusting plate (5), a mounting seat (12) is arranged on each reset mechanism, a fixing mechanism is arranged on the top of the mounting seat (12), a centrifugal tube (13) is fixedly arranged on the top of the mounting seat (12) through the fixing mechanism, and magnetic beads (14) are arranged in the centrifugal tube (13). The top of the base (1) is provided with a reciprocating adjusting mechanism, the reciprocating adjusting mechanism is provided with a lifting block (25), the side of the lifting block (25) is provided with a third magnet block (26), and the third magnet block (26) is used for adsorbing the magnetic beads (14) to realize enrichment and purification of the magnetic beads. The top of the mounting seat (12) is provided with a mounting groove, the lower end of the centrifugal tube (13) is arranged in the mounting groove, the mounting seat (12) is provided with an avoiding groove (21) for inserting the lifting block (25), the avoiding groove (21) is communicated with the mounting groove, so that the third magnet block (26) can be close to the centrifugal tube (13) for magnetic bead enrichment after the lifting block (25) is inserted.

2. The magnetic bead purification device for biological experiments according to claim 1, characterized in that The fixing mechanism comprises a receiving tube (15), a first magnet block (16), an adjusting seat (17), a pressing seat (18), a positioning block (19) and a second magnet block (20), the mounting seat (12) is fixedly provided with the receiving tube (15), the first magnet block (16) is fixedly arranged at the inner bottom of the receiving tube (15), the pressing seat (18) is movably arranged at the top of the adjusting seat (17), the adjusting seat (17) is movably inserted into the adjusting plate (5), the second magnet block (20) is fixedly arranged at the bottom of the adjusting seat (17), and the first magnet block (16) and the second magnet block (20) are adsorbed to each other.

3. The magnetic bead purification device for biological experiments according to claim 2, characterized in that The positioning block (19) is fixedly arranged at the bottom of the pressing seat (18) and is inserted into the centrifugal tube (13).

4. The magnetic bead purification device for biological experiments according to claim 1, characterized in that The reset mechanism comprises a positioning rod (7), a reset spring (8), a sliding block (9) and a pushing block (10), a plurality of sliding grooves (6) are arranged in the adjusting plate (5), the positioning rod (7) is fixedly arranged on the inner wall of the sliding groove (6), the reset spring (8) is sleeved on the positioning rod (7), the sliding block (9) is slidingly arranged on the positioning rod (7), one end of the reset spring (8) abuts against the inner wall of the sliding groove (6), and the other end of the reset spring (8) abuts against the side of the sliding block (9).

5. The magnetic bead purification device for biological experiments according to claim 4, characterized in that The mounting seat (12) is fixedly arranged on the top of the sliding block (9), and the pushing block (10) is fixedly arranged on the bottom of the sliding block (9).

6. The magnetic bead purification device for biological experiments according to claim 5, characterized in that The side of the support (2) is fixedly provided with an air cylinder (11) for pushing the pushing block (10).

7. The magnetic bead purification device for biological experiments according to claim 1, characterized in that The reciprocating adjusting mechanism comprises a fixed frame (22), a second driving motor (23) and a reciprocating screw rod (24), the fixed frame (22) is fixedly installed on the top of the base (1), the second driving motor (23) is fixedly installed on the top of the fixed frame (22), the reciprocating screw rod (24) is fixedly connected on the output shaft of the second driving motor (23) through a shaft coupling, and the lifting block (25) is screwedly installed on the reciprocating screw rod (24).

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